JP5218334B2 - Microorganism removal evaluation apparatus and microorganism removal evaluation method - Google Patents

Description

  The present invention relates to a microorganism removal evaluation apparatus and a microorganism removal evaluation method.

  Conventionally, methods for preventing infectious diseases caused by bacteria and viruses (hereinafter referred to as “microorganisms”) have been studied. Accordingly, methods for sterilization and sterilization of bacteria causing infectious diseases and inactivation of viruses (hereinafter referred to as “microbe removal”) have been studied.

  By the way, the microorganisms to be removed include those that are attached to the surface of furniture, appliances, etc., and those that are attached to water droplets and dust and are suspended in the air. As a method for removing microorganisms floating in the gas phase, a method is generally used in which a gas having a sterilizing power and a property of inactivating viruses is mixed in the gas phase and brought into contact with the microorganism.

  As a method for evaluating the effect of removing such floating microorganisms, for example, a method described in Patent Document 1 has been proposed. That is, in the method of Patent Document 1, an ion generator is installed inside a sealed container having a known volume, and ions having sterilizing power are supplied from the ion generator, and the ions with respect to the microorganisms injected into the container are supplied. The sterilizing power is evaluated. At this time, the amount of ions to be supplied can be changed depending on the driving conditions of the ion generator.

  Alternatively, microorganisms to be evaluated are supplied into the wind tunnel from the upstream side, and ions are supplied to the gas phase by an ion generator installed in the wind tunnel, so that the microorganisms and ions are mixed and brought into contact with each other in the wind tunnel. The microorganisms are removed by a flow-through one-pass method in which microorganisms are collected downstream of the system. On the other hand, a method has been proposed in which microorganisms that have passed through the wind tunnel under the same conditions except that they do not generate ions are collected downstream of the wind tunnel and the microorganism removal performance by ions is evaluated by comparing the amount of microorganisms in both tests. ing.

Japanese Patent No. 4142974

  In general, the microorganism removal performance is evaluated using a “CT value (Concentration-Time Value)” which is the product of the concentration (C) of the substance exposed to the microorganism and the exposure time (T). The CT value is an index indicating the effect of a substance effective in removing microorganisms, and is an index based on the idea that the same microorganism removal effect is obtained if the values are the same.

  Here, when the evaluation using the CT value is performed for the above method, it is difficult to manage the test conditions for the following reason, and the test accuracy may be lowered.

  First, the distribution of test conditions (concentration conditions) within the test environment may cause a decrease in test accuracy. That is, in the method using a sealed container adopted in Patent Document 1, microorganisms and ions are supplied from an injection tube and an ion generator provided on the side wall of the container. In such an apparatus configuration, the injection tube and the ion generator are used. It is easily estimated that the concentration of microorganisms and ions is locally high in the vicinity. Similarly, when the humidity is adjusted using the air conditioning system, there is a possibility that an environment where the humidity is locally high may occur.

  Alternatively, in the circulation type one-pass method employed in Patent Document 1, since ions are supplied from an ion generator arranged upstream of the wind tunnel, ions are consumed and the concentration decreases as it goes downstream of the wind tunnel. . In the apparatus configuration described in Patent Document 1, it is difficult to accurately evaluate the CT value because ions cannot be separately supplied downstream.

  For these reasons, if a distribution occurs in the concentration condition, the evaluation result varies depending on the location in the test environment, and there is a possibility that highly accurate evaluation cannot be performed.

  Further, contamination of the ion generator in the test environment may cause a decrease in test accuracy. That is, in the method described in Patent Document 1, an ion generator that generates ions using an ionization phenomenon such as electric discharge is disposed inside a container or a wind tunnel. In an ion generator employing such a method, contamination of the discharge electrode affects the performance of ion generation. However, the ion generator arranged in the test environment as in Patent Document 1 is derived from the test environment. As a result, the amount of generated ions may change.

  Furthermore, depending on the type of ions and the evaluation conditions, the exposure time for microorganisms may require a long time. However, in the flow-type one-pass method adopted in Patent Document 1, the exposure time is limited by the flow velocity and the length of the wind tunnel. Therefore, the required exposure time cannot be secured, and the evaluation may not be completed. Or in order to ensure a long exposure time, it is necessary to change the length of a wind tunnel, and an efficient evaluation cannot be performed. In addition, when it is necessary to use a long wind tunnel, it is difficult to set the temperature and humidity conditions to certain conditions.

  This invention is made | formed in view of such a situation, Comprising: It aims at providing the microorganism removal evaluation apparatus and microorganism removal evaluation method which enable more accurate evaluation.

  In order to solve the above problems, the microorganism removal evaluation apparatus of the present invention is a microorganism removal evaluation apparatus that measures the ability of microorganisms to sterilize or inactivate microorganisms, and contains the first gas containing the microorganisms therein. Partitioning the first preparation chamber, the second preparation chamber containing the second gas containing the removal substance therein, the first preparation chamber and the second preparation chamber, and opening and closing the first preparation chamber Mixing of the first gas and the second gas obtained by communicating the partition member for controlling communication or blocking between the second preparation chamber and the first preparation chamber and the second preparation chamber And a collection device for collecting a part of the gas.

  According to this configuration, the first gas (microorganism) and the second gas (removed substance) prepared independently in each of the first preparation chamber and the second preparation chamber are mixed in the preparation chamber having a known volume. Therefore, the concentration of microorganisms and the concentration of removed substances, which are test conditions, can be accurately defined. In addition, since the first gas and the second gas diluted in advance in the volume of the first preparation chamber or the second preparation chamber are used, the microorganisms and the removal material are locally present compared to the case where the microorganisms and the removal material are directly supplied to the test chamber. Therefore, it is possible to suppress the occurrence of concentration distribution. Furthermore, since it can be a so-called batch type test in which microorganisms and removal substances are mixed in a preparation room that is a closed space, control of the test time is easier than in the flow type, and the degree of freedom in setting the test time is increased. Increase. By collecting the microorganism mixed with the removal substance in such an apparatus, it is possible to provide a microorganism removal evaluation apparatus capable of performing microorganism removal evaluation under test conditions with a precisely defined concentration.

In the present invention, it is desirable to have a first stirring device that stirs the first gas and uniformly floats the microorganisms in the first preparation chamber.
According to this configuration, since the concentration distribution of the first gas becomes uniform, it is possible to form a mixed gas using the first gas having a uniform concentration and perform more accurate microorganism removal evaluation. Moreover, mixing of the first gas and the second gas can be promoted by the airflow formed by the first stirring device.

In the present invention, it is desirable to have a second stirring device that stirs the second gas and uniformly floats the removed substance in the second preparation chamber.
According to this configuration, since the concentration distribution of the second gas becomes uniform, it is possible to form a mixed gas using the second gas having a uniform concentration and perform more accurate microorganism removal evaluation. Moreover, mixing of the first gas and the second gas can be promoted by the airflow formed by the second stirring device.

In the present invention, it is desirable to have an air conditioning control device that uniformly controls the temperature and humidity inside the first preparation chamber and the temperature and humidity inside the second preparation chamber.
According to this configuration, since the test conditions do not fluctuate when the first gas and the second gas are mixed, it is easy to form desired test conditions, and microorganism removal that enables accurate microorganism removal evaluation is possible. It can be set as an evaluation apparatus.

In the present invention, the first preparation chamber is externally provided so that the concentration of the removal substance in the mixed gas obtained by communicating the first preparation chamber and the second preparation chamber is kept constant. Alternatively, it is desirable to have a removal substance supply device for supplying a removal substance to the second preparation chamber.
Since the removed substance is gradually consumed due to ineffective consumption due to reaction with microorganisms or natural decomposition, it decreases from the initial concentration. For this reason, when the test time takes a long time or when the attenuation width of the removed substance is large, the concentration condition of the removed substance is different between the initial stage of the test and the late stage of the test. However, according to this configuration, since the removal substance can be appropriately added by the removal substance supply apparatus, the microorganism removal evaluation apparatus capable of performing the microorganism removal evaluation while suppressing the change in the test conditions due to the concentration attenuation of the removal substance, and can do. Moreover, by providing the removal substance supply device outside the first preparation chamber and the second preparation chamber for performing the test, the contamination of the removal substance supply device by the contaminants derived from the test such as microorganisms can be suppressed and removed. A substance can be supplied stably.

In the present invention, the removal substance supply device accommodates the third gas containing the removal substance therein, and opens and closes a partition member disposed between the first preparation chamber or the second preparation chamber, It is desirable to have a third preparation chamber capable of controlling communication or blocking with the first preparation chamber or the second preparation chamber.
According to this configuration, by preparing a removal substance whose concentration is precisely defined in the third preparation chamber, the partition member is opened and connected in accordance with the attenuation of the removal substance, and concentration control is performed from the third preparation chamber. The removed removal material can be supplied. Therefore, it can be set as the microorganism removal evaluation apparatus which can perform microorganism removal evaluation which suppressed the change of the test conditions by the density | concentration attenuation | damping of a removal substance.

In this invention, it is desirable to have the said microorganisms supply means which sprays the suspension liquid which suspended the said microorganisms in the liquid inside the said 1st preparation chamber.
According to this configuration, a very small amount of microorganisms can be easily supplied into the first preparation chamber depending on the concentration of the suspension and the spray amount, and the concentration can be accurately controlled. Further, when the suspension medium is water, the humidity in the first preparation chamber can be adjusted at the same time.

  Further, the microorganism removal evaluation method of the present invention is a microorganism removal evaluation method for measuring the ability of a microorganism to sterilize or inactivate microorganisms, wherein the microorganism and the removal substance are each independently specified in the gas phase. A mixture treatment in which both are mixed together to remove microorganisms, and a part of the mixed gas in which the microorganisms and the removal substance are mixed is collected, and the microorganisms contained in the collected mixed gas are collected. And a recovery process for recovery and an evaluation process for evaluating the microorganism removal performance of the removed substance based on the activity of the recovered microorganisms.

  According to this method, it is possible to provide a microorganism removal evaluation method capable of performing microorganism removal evaluation under accurate concentration conditions by mixing a microorganism prepared at a predetermined concentration and a removal substance independently.

In the present invention, prior to the recovery process, it is desirable to perform an additional process of measuring the concentration of the removed substance contained in the mixed gas and adding the removed substance having a predetermined concentration.
According to this method, since the concentration of the removed substance can be controlled as necessary, the concentration management of the removed substance is prepared. In addition, if the removed substance attenuates due to ineffective consumption, it is difficult to maintain the test for a long time, but this method can add a sufficient amount of the removed substance at an accurate concentration. It can be set as the microorganism removal evaluation method in which removal evaluation is possible.

  According to the present invention, it is possible to provide a microorganism removal evaluation apparatus and a microorganism removal evaluation method capable of performing microorganism removal evaluation with higher accuracy than conventional ones under the test conditions in which the concentration is accurately defined.

It is a schematic explanatory drawing which shows the microorganism removal evaluation apparatus which concerns on 1st Embodiment. It is a schematic explanatory drawing which shows the microorganism removal evaluation method of this invention. It is the schematic which shows the microorganisms removal evaluation apparatus which concerns on 2nd Embodiment. It is the schematic which shows the modification of the microorganisms removal evaluation apparatus which concerns on 2nd Embodiment.

[First Embodiment]
Hereinafter, the microorganism removal evaluation apparatus and the microorganism removal evaluation method according to the first embodiment of the present invention will be described with reference to FIGS. In all the drawings below, the dimensions and ratios of the constituent elements are appropriately changed in order to make the drawings easy to see.

  FIG. 1 is a schematic explanatory view showing a microorganism removal evaluation apparatus 100A according to the present embodiment. As shown in the figure, the microorganism removal evaluation apparatus 100A of this embodiment includes a chamber 100, a microorganism supply means 3 connected to the chamber 100, a removal substance supply means 4, and a collection device 5.

  In the present invention, “microorganism” includes fungi, bacteria and viruses. The “removal substance” refers to a substance having the property of sterilizing or sterilizing these microorganisms if they are fungi or bacteria, and inactivating them if they are viruses. Here, an influenza virus is taken as an example of microorganisms and ozone is taken as an example of a removal substance, and the following explanation is given.

  The chamber 100 has a plurality of partition doors (partition members) 110 inside, and the interior of the chamber 100 is divided into a plurality of (four in the figure) small chambers by the partition door 110. It is desirable that the inner surface of the chamber 100 be formed of a material that can cope with a reliable killing process, such as glass, fluororesin, and stainless steel.

  The partition door 110 is provided so that it can be opened and closed, and the small chambers can be connected to each other as necessary. As the partition door 110, a generally known mechanism such as a butterfly valve or a gate valve can be used. For example, a sanitary powder butterfly valve manufactured by Osame Kogyo can be exemplified.

  One of the small chambers divided by the partition door 110 is used as a floating preparation chamber (first preparation chamber) 1 in which influenza virus is floated in advance at a predetermined concentration, and the other small chambers are in ozone with a predetermined concentration. Is used as an ozone preparation chamber (second preparation chamber) 2. In the present embodiment, a small chamber disposed at one end of the chamber 100 is a floating preparation chamber 1, an ozone preparation chamber 2A is provided adjacent to the floating preparation chamber 1, and an ozone preparation chamber (first) is adjacent to the ozone preparation chamber 2A. 3 preparation chamber) 2B, and ozone preparation chamber (third preparation chamber) 2C is provided adjacent to ozone preparation chamber 2B.

  A microorganism supply means 3 is connected to the floating preparation chamber 1. The microorganism supply means 3 has an aspirator 31 that sucks and sprays liquid by air pressure, and a storage tank 32 that stores a suspension 3x of influenza virus, and uses the air pressure to suck up the suspension 3x to prepare for floating. The suspension 3x is sprayed into the chamber 1. When the suspension medium is water, the microorganism supply means 3 is configured to function as a humidifying means (air conditioning control device) for humidifying the inside of the floating preparation chamber 1 together with the supply of influenza virus.

  As the aspirator 31, one that can be easily sterilized is desirable, and for example, a fluororesin aspirator manufactured by Bee Manufacturing can be exemplified.

  The floor surface of the floating preparation chamber 1 is provided with a wing-shaped first stirring device 11 that stirs the inside. Similarly, the floor surface of the ozone preparation chamber 2 has a wing-shaped second stirring device 21 that stirs the inside. Is provided. Here, the magnetic stirrer 12 provided outside the chamber 100 moves the respective stirring devices 11 and 21 in a non-contact manner to stir the internal gas phase. These configurations are intended to prevent spraying of the sprayed influenza virus suspension or settling of the influenza virus and to float it uniformly in the gas phase.

  Assuming that the droplet of influenza virus suspension 3x supplied into the floating preparation chamber 1 has a diameter of 3 to 5 μm, the size of the influenza virus is 0.1 μm, and the specific gravity is assumed to be 1, the sedimentation rate is calculated from the stokes equation. Is calculated as 0.7 mm / s. Therefore, the first stirrer 11 and the second stirrer 21 are driven so as to generate an updraft that is higher than the settling velocity.

  Each ozone preparation chamber 2 is connected with ozone supply means (removed substance supply means) 4. The ozone supply means 4 includes an ozone generator 41, a humidifying tank 42 that humidifies a gas containing ozone, and a demister 43 connected in series to the humidifying tank 42 to remove excess water droplets, and each ozone preparation Ozone can be supplied through a pipe connected to the chamber 2 via a valve 44. That is, the ozone supply means 4 is configured to function as a humidifying means as necessary. Moreover, the ozone supply means 4 connected to the outside of the ozone preparation chamber 2 can stably supply ozone without being contaminated by contaminants derived from the test environment.

  In addition, since whether or not to humidify the gas containing ozone generated by the ozone generator 41 can be selected by the switching valves 45 and 46, only the gas containing ozone is supplied into the ozone preparation chamber 2 without being humidified. It is also possible to do. Furthermore, for example, it is possible to adjust the humidity in the ozone preparation chamber 2 by connecting an air pump (not shown) or the like to the humidification tank 42 and supplying only air.

  A collection device 5 is connected to the floating preparation chamber 1. The sampling device 5 is provided so as to protrude from the floor surface of the floating preparation chamber 1, and is connected to the sampling tube 51a for collecting the internal gas together with the floating microorganisms, and the collecting members 51 and 52 for collecting the microorganisms. And a suction pump 53 for sucking the gas in the floating preparation chamber 1 through the sampling tube 51a.

  As the recovery member 51, a generally known gelatin filter can be used. As the recovery member 52, in order to reliably recover a trace amount of virus contained in the exhaust gas that has permeated through the gelatin filter, it is recovered by bubbling in the liquid. It is possible to use a trap tube or the like. The recovery member 52 is connected to a storage tank 54 in which a thiosulfuric acid solution for neutralizing and decomposing ozone is stored, and is configured to decompose ozone contained in the liquid after recovering the microorganisms.

  A decomposition device 55 that decomposes ozone by heat or activated carbon adsorption may be provided on the downstream side of the suction pump 53. This decomposing apparatus 55 can be used for decomposing ozone remaining in the chamber 100 after completion of the test by being connected to the chamber 100 through the valve 56.

  Further, the microorganism removal evaluation apparatus 100A is provided with a temperature / humidity monitor 61 for measuring the temperature and humidity in the floating preparation chamber 1 and the ozone preparation chamber 2, and an ozone monitor 62 for measuring the internal ozone concentration. In the ozone monitor 62, the gas in the chamber 100 is taken in through the valve 62a, the ozone concentration is measured, and the measured gas is returned into the chamber 100 through the valve 62b. Yes.

  The measurement result by each monitor is transmitted to the control device 7, and the heater (air conditioning control device) 63 provided in the ozone generator 41 and the chamber 100, the air pump (not shown) connected to the humidifying tank 42, and the like are controlled. The temperature, humidity, and ozone concentration of the floating preparation chamber 1 and the ozone preparation chamber 2 are controlled. Since the floating preparation chamber 1 and the ozone preparation chamber 2 are closed spaces, uniform test conditions can be obtained by controlling the temperature, humidity, and ozone concentration, and the microorganism removal test can be performed under well-controlled test conditions. Can be implemented.

  A suction pump 53 is also connected to the control device 7 and controls the operation of the suction pump 53. Specifically, based on the information on the open / closed state of the partition door 110 separately transmitted to the control device 7, the space volume of the connected floating preparation chamber 1 and the ozone preparation chamber 2A, or further the ozone preparation chambers 2B and 2C are added. In order to adjust the sampling amount in accordance with the space volume, the operation of the suction pump 53 is controlled.

In order to prevent contact due to leakage of influenza virus, the chamber 100, the microorganism supply means 3, and the recovery members 51 and 52 are preferably installed in the draft 8.
The microorganism removal evaluation apparatus 100A of the present embodiment is configured as described above.

  Next, a microorganism removal evaluation method using the microorganism removal evaluation apparatus 100A of this embodiment will be described with reference to FIG. For ease of explanation, only a part of the microorganism removal evaluation apparatus 100A is shown in the figure.

  In addition, it goes without saying that a blank test and a positive control are performed as in the normal test. In addition, the natural decomposition amount of ozone is also evaluated in advance.

  First, as shown in FIG. 2 (a), using the microorganism supply means 3 (not shown), the suspension preparation chamber 1 is sprayed with droplets of the suspension 3x containing influenza virus, and the suspension preparation chamber 1 has the volume. A first gas in which diluted influenza virus having a predetermined concentration is suspended is prepared. The first stirrer 11 operates on the floor surface of the floating preparation chamber 1 to prevent droplets from sinking.

  Similarly, ozone 4x is supplied into the ozone preparation chamber 2A using the ozone supply means 4, and a second gas having a predetermined ozone concentration diluted with the volume of the ozone preparation chamber 2A is prepared in the ozone preparation chamber 2A. . On the floor surface of the ozone preparation chamber 2, the second stirring device 21 is operated to promote the uniform ozone concentration.

  Inside the floating preparation chamber 1 and the ozone preparation chamber 2A, the temperature and humidity are controlled by an air conditioning control device (not shown), and the temperature and humidity of the first gas and the second gas are kept uniform in advance.

  Next, as shown in FIG. 2B, the partition door 110 that partitions between the floating preparation chamber 1 and the ozone preparation chamber 2A is opened, and the spaces of the two preparation chambers are connected (mixing process). A space obtained by combining the connected floating preparation chamber 1 and the ozone preparation chamber 2 </ b> A is a test chamber 10.

  When the two preparation chambers are connected, the first gas and the second gas are mixed in the test chamber 10 to form a mixed gas. That is, the suspended droplet 3x (or influenza virus) or ozone 4x diffuses and has a uniform concentration in the test chamber 10. The test for removing microorganisms is performed at the concentration that is uniform in the test chamber 10. The first stirrer 11 and the second stirrer 21 operate on the floor surface in the test chamber 10 to prevent sedimentation of the droplets of the suspension 3x and promote the mixing of the first gas and the second gas. Cause it to occur. Since the first gas and the second gas have the same temperature and humidity, these test conditions do not vary during mixing.

  Here, since the concentration of the dispersion 3x drops and the concentration of the ozone 4x is lowered by the spread of the diffusion volume, the influenza virus and the ozone are preliminarily set in the floating preparation chamber 1 and the ozone preparation chamber 2A at a concentration considering the decrease in concentration. Adjust the density. Further, it is preferable to set the diffusion conditions in advance by a preliminary experiment or the like so that the diffusion is completed within a short time with respect to the entire test time.

  Next, as shown in FIG. 2 (c), after a predetermined time has elapsed from mixing of the suspension 3x droplets (influenza virus) and ozone 4x, that is, after the influenza virus has been exposed to ozone for a predetermined time. Then, the suction pump 53 is operated to collect the influenza virus (collection process).

  The gas (mixed gas) in the test chamber 10 sucked by the suction pump 53 includes ozone, influenza virus, and inactivated influenza virus. Among these, influenza virus and inactivated influenza virus are used. Recovered by the recovery members 51 and 52.

  The collected virus is cultured by a generally known method, and the infectious titer is measured (measurement process). The concentration of ozone exposed until the virus is recovered can be accurately determined by the amount of ozone prepared in the ozone preparation chamber 2A and the volume of the test chamber 10, and the time of ozone exposure before the virus is recovered is also accurate. Can be requested. Therefore, the CT value from which the measured infectious titer was obtained can be accurately obtained.

Further, when it is necessary to add ozone during the test in order to adjust the ozone concentration, a necessary amount of ozone 4x is supplied into the ozone preparation chamber 2B adjacent to the test chamber 10, and the test chamber 10 It adds by opening the partition door 110 between ozone preparation chambers 2B. In this case, a space combining the test chamber 10 and the ozone preparation chamber 2B becomes a new test chamber.
As described above, the microorganism removal evaluation method of the present embodiment can be performed.

  Therefore, according to the microorganism removal evaluation apparatus 100A configured as described above, a microorganism removal evaluation apparatus capable of performing microorganism removal evaluation under test conditions in which the virus concentration and the ozone concentration are accurately defined can be obtained.

  Moreover, according to the microorganism removal evaluation method having the above-described configuration, the microorganism removal evaluation can be performed under an accurate concentration condition by mixing the microorganisms and the removal substances independently prepared at a predetermined concentration. It can be a microorganism removal evaluation method.

  In the present embodiment, the inside of the chamber 100 is humidified by the microorganism supply means 3 and the ozone supply means 4, but a humidifying means for spraying sterilized water to humidify the inside of the chamber 100 may be provided separately.

[Second Embodiment]
FIG. 3 is an explanatory diagram of a microorganism removal evaluation apparatus 101A according to the second embodiment of the present invention. The microorganism removal evaluation apparatus 101A of this embodiment is partially in common with the microorganism removal evaluation apparatus 100A of the first embodiment. The main differences are the shape of the chamber and the arrangement of the partition doors that partition the chamber. Therefore, in this embodiment, the same code | symbol is attached | subjected about the component which is common in 1st Embodiment, and detailed description is abbreviate | omitted. Further, here, for ease of explanation, only the chamber and the configuration inside the chamber are shown, and the incidental equipment connected outside the chamber is omitted.

  The chamber 101 included in the microorganism removal evaluation apparatus 101A of the present embodiment has a shape showing a substantially cross in plan view. The inside of the chamber 101 is partitioned by four partition doors 110, and the floating preparation chamber 1 at a position corresponding to the crossing portion of the cross, and the four ozone preparation chambers 2 at positions corresponding to the ends of the cross, , Is formed. That is, each ozone preparation chamber 2 can be connected to the floating preparation chamber 1 independently.

  A first stirring device 11 is disposed on the floor surface of the floating preparation chamber 1, and a second stirring device 21 is disposed on the floor surface of each ozone preparation chamber 2. Further, a separate stirring fan 23 is provided on the side surface of the ozone preparation chamber 2 facing the partition door 110. When the partition door 110 is opened, the stirring fan 23 forms a convection that sends ozone prepared in the ozone preparation chamber 2 to the floating preparation chamber 1 side, and microorganisms floating in the floating preparation chamber 1 and ozone It has a function of promoting diffusion and mixing with ozone floating in the preparation chamber 2.

  In the microorganism removal evaluation apparatus 101A having the above-described configuration, the ozone preparation chambers 2 are closer to each other than the chambers 100 of the first embodiment in which a plurality of ozone preparation chambers 2 are connected in series. Thus, when ozone is newly added, the time required for ozone diffusion can be shortened. Therefore, it is possible to shorten a state where the concentration until ozone diffuses is shortened, and it is possible to provide a microorganism removal evaluation apparatus capable of performing more accurate microorganism removal evaluation.

  In the present embodiment, four ozone preparation chambers 2 are provided adjacent to the side wall of the floating preparation chamber 1. However, the ozone preparation chamber 2 can be increased as necessary.

  As in FIG. 3, the ozone preparation chamber 2 may be adjacent to the side wall of the floating preparation chamber 1. For example, the ozone preparation chamber 2 may be provided on the floor side or the ceiling side of the floating preparation chamber 1. Further, like the chamber 102 provided in the microorganism removal evaluation apparatus 102A shown in FIG. 4, the point adjacent to the side wall of the floating preparation chamber 1 is the same as in FIG. 3, and the two chambers 101 in FIG. The ozone preparation chambers may be stacked, and a plurality of (eight in the figure) ozone preparation chambers 2 may be disposed around the central floating preparation chamber 1.

  The preferred embodiments of the present invention have been described above with reference to the accompanying drawings, but it goes without saying that the present invention is not limited to such examples. Various shapes, combinations, and the like of the constituent members shown in the above-described examples are examples, and various modifications can be made based on design requirements and the like without departing from the gist of the present invention.

DESCRIPTION OF SYMBOLS 1 ... Floating preparation chamber (1st preparation chamber), 2A ... Ozone preparation chamber (2nd preparation chamber), 2B-2C ... Ozone preparation chamber (3rd preparation chamber), 3 ... Microorganism supply means, 3x ... Suspension, DESCRIPTION OF SYMBOLS 4 ... Ozone supply means (removal substance supply means), 5 ... Extraction apparatus, 11 ... 1st stirring apparatus, 21 ... 2nd stirring apparatus, 63 ... Heater (air-conditioning control means), 100A-102A ... Microorganism removal evaluation apparatus, 110 ... Partition door (partition member),

Claims (9)

A microorganism removal evaluation apparatus for measuring the ability of a substance to sterilize or inactivate microorganisms,
A first preparation chamber for accommodating therein a first gas containing the microorganism;
A second preparation chamber for accommodating therein a second gas containing the removal substance;
A partition member that partitions the first preparation chamber and the second preparation chamber, and controls communication or blocking between the first preparation chamber and the second preparation chamber by opening and closing thereof;
A microorganism having a collection device for collecting a part of a mixed gas of the first gas and the second gas obtained by communicating the first preparation chamber and the second preparation chamber Removal evaluation device.   The microorganism removal evaluation apparatus according to claim 1, further comprising a first stirring device that stirs the first gas and uniformly floats the microorganisms in the first preparation chamber.   3. The microorganism removal evaluation apparatus according to claim 1, further comprising a second stirring device that stirs the second gas and uniformly floats the removed substance in the second preparation chamber.   4. The air conditioning control apparatus according to claim 1, further comprising an air conditioning controller that uniformly controls the temperature and humidity inside the first preparation chamber and the temperature and humidity inside the second preparation chamber. 5. The microorganism removal evaluation apparatus according to item.   The first preparation chamber or the second preparation is externally provided so that the concentration of the removal substance in the mixed gas obtained by communicating the first preparation chamber and the second preparation chamber is kept constant. The microorganism removal evaluation apparatus according to any one of claims 1 to 4, further comprising a removal substance supply device that supplies the removal substance to the chamber.   The removal substance supply device accommodates a third gas containing the removal substance therein and opens and closes the first preparation chamber by opening and closing a partition member disposed between the first preparation chamber and the second preparation chamber. The microorganism removal evaluation apparatus according to claim 5, further comprising a third preparation chamber capable of controlling communication or blocking with the second preparation chamber.   5. The microorganism removal according to claim 1, further comprising: the microorganism supply unit that sprays a suspension in which the microorganism is suspended in a liquid in the first preparation chamber. Evaluation device. A microorganism removal evaluation method for measuring the ability of microorganisms to sterilize or inactivate microorganisms,
A mixing process in which the microorganisms and the removal substance are each independently suspended at a predetermined concentration in the gas phase, and then mixed to remove the microorganisms.
Collecting a part of a mixed gas in which the microorganism and the removal substance are mixed, and collecting the microorganism contained in the collected mixed gas;
A microorganism removal evaluation method, comprising: performing an evaluation process for evaluating the microorganism removal performance of the removed substance based on the activity of the collected microorganisms.   The microorganism removal evaluation method according to claim 8, wherein prior to the recovery process, an additional process of measuring the concentration of the removal substance contained in the mixed gas and adding the removal substance having a predetermined concentration is performed. .

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