JP5135152B2 - Chamber device - Google Patents

Description

  The present invention relates to a chamber device for non-human primates used in medical and pharmaceutical tests.

  Conventionally, in medical and pharmaceutical tests using animals such as monkeys, methods for measuring the central nervous system and cardiovascular system without anesthesia and without restraint have been established. However, when measuring the respiratory system, a test was performed under anesthesia or restraint such as a monkey chair. However, under the environment as described above, the effects of anesthesia and restraint stress are applied to the animals, and thus the above test could not be performed on animals in a normal state. In particular, in respiratory and cardiovascular tests in pharmacological tests, the heart rate and blood pressure may be measured at the same time. It was desired to conduct a test.

When performing a respiratory system test, a method is used in which an animal is housed in a sealed transparent chamber and the respiration rate of the animal is sensed by a change in atmospheric pressure in the chamber (see Patent Document 1). ) In such chambers, monkeys are difficult to acclimate and put in and out of the chamber, so they had to be tested using small animals such as mice, rats, and guinea pigs that could be acclimatized and easily put in and out of the chamber. . As a respiratory system testing apparatus that solves this problem, an animal such as a monkey is housed in a cage surrounded by a metallic lattice that enables internal observation and air flow, and the cage containing the animal is sealed with the above-mentioned seal. A chamber apparatus having a configuration of being accommodated in a transparent chamber is disclosed (see Patent Document 2). According to the chamber apparatus, it is described that an animal can be acclimatized by accommodating the animal in the cage, and a pharmacological test can be performed without anesthesia and without restraint.
Special table 2007-500067 gazette International Publication No. WO2007-015289

  Conventionally, an animal such as a monkey is accommodated in a cage surrounded by a metallic lattice that enables internal observation and air circulation, and the cage containing the animal is contained in the sealed transparent chamber. A chamber apparatus has been developed. In the chamber apparatus, a metal cage is required for each chamber, so that the apparatus is expensive, detailed observation of animals from outside is difficult, and the inside of the chamber is difficult. There is a problem that the natural flow of air generated by ventilation and breathing is hindered by the presence of fistula. In addition, metal plates usually used for animal cages are easy to bend because of the thin plates used for transportation, such as weight, and animals move around the cage and touch the walls of the cage. There is a problem that the movement is transmitted to the ridge, an air flow affecting the measurement is generated, and the operation in the measurement becomes complicated.

  Therefore, in the present invention, it is an object to provide a chamber apparatus having a simple configuration that can be carried out in an unanesthetized and unrestrained state in medical and pharmaceutical measurements using animals such as monkeys. And

In order to solve the above-described problems, the present invention has been devised in that an animal such as a monkey is seated on a protrusion by installing a protrusion on the side surface of the chamber apparatus. As a result, animals such as monkeys (hereinafter referred to as “non-human primates”. In the present specification, “monkeys” mean an example of “non-human primates”) are contained in the chamber apparatus. It is possible to provide a chamber apparatus that can be quietly rested.

  Specifically, the chamber apparatus according to the present invention is a non-anesthetized, non-restrained measurement chamber apparatus for a non-human primate, and includes a storage unit that stores the non-human primate, and an interior of the storage unit. And a protrusion on which a non-human primate can be seated.

  The non-human primate housed in the chamber apparatus takes a long time to sit on the protrusion, and is considered to be able to spend more time than when housed in a chamber apparatus without a protrusion. It was issued. Therefore, the chamber apparatus according to the present invention is capable of resting the non-human primate by providing a protrusion on the inner side surface of the housing part, and the non-human primate is unanesthetized and unrestrained. It is possible to measure in a state close to the natural state.

  Here, the chamber apparatus according to the present invention is provided with an opening for a non-human primate to enter and exit from the outside to the housing part, and a non-human being installed through the opening by being opened and closed outside the opening. And a door part that adjusts the entry and exit of the primate into and from the housing part, and the door part is installed to face the opening, and the non-human primate in the opening A first door that can be pulled out in a direction perpendicular to the entrance / exit direction, and is installed on the opposite side of the opening with the first door interposed therebetween, and the housing portion and the outside through the opening You may make it have the 2nd door which can interrupt | block in / out of air.

  When the measurement is performed in the chamber device, the non-human primate is moved from the breeding cage to the transport cage, and further moved from the transport cage to the housing portion of the chamber device. On the other hand, when the measurement in the chamber apparatus is completed, the non-human primate is moved from the storage section to the transport cage and moved from the transport cage to the breeding cage. While such a use form is assumed, the chamber apparatus according to the present invention includes a first door that constitutes a door portion and can be pulled out in the vertical direction, and is further installed on the first door. Having two doors facilitates movement between the non-human primate chamber apparatus and the transport cage. Here, since the first door is configured so that it can be pulled out in a direction perpendicular to the direction of entry and exit of the non-human primate, the transport cage and the non-human primate are connected to each other to connect the non-human primate. It is possible to prevent it from escaping when it is moved. More specifically, for example, when a non-human primate is present in the chamber apparatus, the second door of the chamber apparatus is opened with the first door closed. Then, the door of the transport cage is opened, and the opening of the chamber device and the opening of the transport cage are connected to face each other. Then, by pulling out the first door in the vertical direction, a state is formed in which the transfer cage and the chamber apparatus can be moved while being held in a connected state. Therefore, it is possible to move non-human primates to the transport cage.

  In addition, the second door in the chamber apparatus according to the present invention can block air from entering and exiting through an opening through which a non-human primate provided in the housing part enters and exits. Therefore, the chamber apparatus according to the present invention can obtain a more accurate measurement value at the housing portion by blocking the air from entering and exiting the housing portion that affects the measurement. The second door may be installed detachably with respect to the accommodating portion. In such a case, when the non-human primate is moved between the chamber device and the transport cage, the second door After removing the door, the above-described first door may be pulled out.

Moreover, the chamber apparatus which concerns on this invention may be equipped with the accessory required when accommodating a non-human primate between said 1st door and said 2nd door. The accessory is, for example, an infusion pump, a chemical solution, a food box, or a water supply device necessary for measurement. An accessory is installed between the first door and the second door, which is necessary to contain the non-human primate while maintaining the sealed state of the opening where the non-human primate enters and exits. Accessories and the like can be easily installed.

  Here, the chamber apparatus according to the present invention is a chamber apparatus that performs a pharmacological test of a respiratory system of a non-human primate, and the storage unit is separated from a test room in which a pharmacological test is performed and the test room. The test chamber and the reference chamber may be communicated with each other by a communication pipe that has a reference chamber that is arranged in parallel with the pharmacological test of the test chamber and supplies external air.

  The chamber apparatus according to the present invention has both a test room and a reference room, so that a non-human primate to which a drug has been administered is accommodated in the test room, and the test room for performing the pharmacological test is compared with the reference room. Measurement errors can be reduced. Therefore, it is possible to carry out a pharmacological test with little measurement error under the same environment where a non-human primate is housed in the housing part. Specifically, the respiration of animals is measured by the difference between the laboratory pressure and atmospheric pressure, but using only the atmospheric pressure in the laboratory will introduce noise and will not be accurate. By using the measured value of the atmospheric pressure of the reference chamber that is sealed (not easily affected by sudden fluctuations in the outside air), stable atmospheric pressure can be measured and measurement noise can be reduced.

  Here, in the chamber apparatus according to the present invention, the non-human primate may be a monkey. Since monkeys have similar biological functions to humans, the ability to perform medical and pharmaceutical measurements under anesthesia and unrestraint using monkeys is advantageous in predicting the effects of drugs, etc. on humans.

  The chamber apparatus according to the present invention provides a chamber apparatus having a simple configuration and capable of performing this in an unanesthetized and unconstrained state in medical and pharmaceutical measurements using animals such as monkeys. Is possible.

  Here, an embodiment of the chamber apparatus 1 according to the present invention will be described with reference to the drawings. Below, taking the respiratory system measuring apparatus as an example, the chamber apparatus 1 of the present invention will be described based on the drawings, but the chamber apparatus 1 of the present invention is not limited to the following. FIG. 1 is a perspective view of a chamber apparatus 1 according to an embodiment of the present invention. FIG. 1A is an AA enlarged sectional view of the communication pipe 26 of the chamber apparatus 1 according to the embodiment of the present invention. FIG. 2 is a front perspective view in which the double door 4 of the chamber apparatus 1 according to the embodiment of the present invention is opened. In the examples of the present invention, monkeys are exemplified as non-human primates.

(1) Configuration of Chamber Device The chamber device 1 according to the embodiment of the present invention is a measurement chamber device in a non-anesthetized and unrestrained state of a monkey, and includes a first housing portion 2 for housing a monkey, and a first housing. A projection 3 capable of seating a monkey on the side of the interior of the test chamber 21 of the section 2; and a door 4 that allows the monkey to enter and exit the test chamber 21 of the first housing section 2 by opening and closing. . The chamber apparatus 1 according to the embodiment of the present invention may be any apparatus that performs measurement for medical and pharmaceutical tests using a monkey housed in the test chamber 21 of the first housing unit 2. Specific examples include measuring devices for respiratory system, metabolic system, circulatory system, and central nervous system pharmacology test using monkeys. Among these pharmacological tests, the chamber apparatus 1 of the embodiment of the present invention is preferably used in the pharmacological tests of the respiratory system that are particularly affected by monkey's momentum, stress and the like.

As shown in FIG. 1, the chamber apparatus 1 according to the embodiment of the present invention is a rectangular parallelepiped, and the upper surface 51 and the front surface 52 are surrounded by a transparent wall, and the side surface 53, the back surface 54, and the bottom surface 55 are surrounded by an opaque wall. Yes. In addition, the upper surface 51 and the front surface 52 should just be comprised by the transparent wall. The chamber apparatus 1 has a structure capable of blocking the entry and exit of air inside and outside the chamber apparatus 1. Since the monkey may be in direct contact with the transparent wall on the upper surface 51, it is preferable that the wall has strength. Specifically, an acrylic plate having a thickness of 10 mm or more, or a transparent plate such as polyvinyl chloride or polycarbonate is preferably used. It is also preferable to use a stainless steel frame installed so as to fit in the frame. As shown in FIG. 2, a releasable metal plate 6 that can be inserted and removed is installed on the bottom surface of the test chamber 21 of the chamber apparatus 1. In addition, when moving an animal to the transport cage 100 after the measurement, a transport cage insertion rail 61 is installed on the bottom surface of the test chamber 21. The transport cage 100 is a cage used when transporting monkeys from the breeding cage to the chamber apparatus 1. A description of the transport cage 100 will be described later.

  Moreover, as shown in FIG. 1, the door part 4 comprised by the 1st door 41 and the 2nd door 42 is installed in the front surface 52 of the chamber apparatus 1 of the Example of this invention. The first door 41 and the second door 42 are doors that can be opened and closed with respect to the second opening 7 in order to allow monkeys to enter and exit the first accommodating portion 2 of the chamber apparatus 1. . Specifically, the first door 41 is installed so as to face the second opening 7, and can be pulled out in a direction perpendicular to the entrance / exit direction of the monkey at the second opening 7. . The second door 42 is installed on the opposite side of the second opening 7 across the first door 41, that is, further overlaps the first door 41 from the outside of the chamber device 1. While being installed, it is possible to block the entry and exit of air between the first housing portion 2 and the outside through the second opening 7.

  Since the first door 41 can be pulled out in the direction perpendicular to the second opening 7, the movement of the monkey from the chamber device 1 to the carrying cage 100 becomes convenient, and the monkey is carried from the chamber device 1. It is possible to prevent escape when moving to the cage 100. The second door 42 has a rubber so that when the second door 42 is closed, the second opening 7 is hermetically sealed so that air can be prevented from entering and exiting inside and outside the chamber apparatus 1. It is preferable to apply packing such as. Packing such as rubber may be applied to either the first accommodating portion 2 side or the second door 42 side, but the second door 42 side is preferred. The first door 41 can be pulled out in the vertical direction when the second door 42 is removed.

  The first door 41 can be made of the transparent plate, but is made of a metal plate with holes, if necessary. Such a first door 41 is convenient for fixing an infusion tube, for example, in the case of measurement in which a medicine is continuously administered to a monkey. Further, in this case, the infusion pump and the chemical solution may be installed between the first door 41 and the second door 42, outside the test chamber 21 of the first storage unit 2 for storing monkeys, or outside the chamber apparatus 1. Is possible. In the case where an infusion pump or a chemical solution is installed outside, a hole having a structure that does not allow air to enter and exit from the second door 42 can be opened and the tube can be passed through. In addition, by installing an infusion pump or a chemical solution between the first door 41 and the second door 42, it is possible to easily maintain the sealed state of the second opening 7 through which the monkey enters and exits. . Specific operations of the first door 41 and the second door 42 will be described later.

  Further, the chamber device 1 is provided with a monkey bait box 8 under the first housing portion 2, and further, a water supply device 9 is also installed between the first door 41 and the second door 42. (See FIG. 1). The test chamber 21 is preferably ventilated, and a vent (not shown) connected to a ventilator installed outside the test chamber 21 is installed, and the vent vents the air in the chamber apparatus 1. In order to ventilate efficiently, it is preferable to install at a plurality of locations on the upper and lower sides of the left and right side surfaces (not shown). As the respiratory system measuring apparatus installed in the chamber apparatus 1 of the embodiment of the present invention, for example, the apparatus described in JP-T-2007-500067 is installed.

The chamber apparatus 1 has a first accommodating portion 2 that accommodates monkeys, and an apparatus necessary for measurement is installed. One or a plurality of protrusions 3 on which a monkey can be seated, which is a feature of the chamber apparatus 1 according to the embodiment of the present invention, are installed on any one of the four side surfaces of the test chamber 21 of the first accommodating portion 2 of the monkey. The projection 3 may have any size and shape as long as the monkey used for measurement can be seated, but in the case of a cynomolgus monkey, a square or ellipse of 10 cm × 10 cm to 30 cm × 30 cm may be used. preferable. Further, the place where the protrusion 3 is installed is not particularly limited as long as the monkey is seated and stable, but the monkey is located on the opposite side of the double door 4 or the furthest away from the direction in which the person passes. It is preferable to install in a place where a sufficient space above is secured. One protrusion 3 of the chamber apparatus 1 according to the embodiment of the present invention is installed on the side surface in the test chamber 21 of the first housing 2 facing the double door 4. Examples of the material of the protrusion 3 include acrylic, wood, vinyl chloride, polycarbonate, and the like, and vinyl chloride is preferable. The color is not particularly limited, but a dark color such as black or gray is preferable.

  The test chamber 21 of the first accommodating portion 2 of the chamber apparatus 1 according to the embodiment of the present invention includes a test chamber 21 that accommodates monkeys and has a first air flow opening 23, and a reference chamber 22 that has a second air flow opening 24. (See FIG. 1A). In the laboratory 21, a pharmacological test of the respiratory system of monkeys is performed. Animal respiration is measured by the difference between the laboratory pressure and atmospheric pressure, but if only the atmospheric pressure in the laboratory is used, noise will be introduced and will not be accurate. By using the measured value of the atmospheric pressure in the reference chamber, which is not easily affected by fluctuations, a stable atmospheric pressure can be measured and measurement noise can be reduced. The 1st airflow opening 23 and the 2nd airflow opening 24 are connected by the communication pipe 26 which supplies external air from the external airflow opening 25 (refer FIG.1 and FIG.1A). The test chamber 21 and the reference chamber 22 are separated by a common separation wall 27, and it is desirable that all of the test chamber 21, the reference chamber 22 and the separation wall 27 are constituted by the transparent plate or the like. .

The size of the test chamber 21 is defined by the guidelines for animal experiments of ILAR (Institute of Laboratory Animal Resources) according to the length of the monkeys to be accommodated, and the monkeys weighing 3 kg to 10 kg have a floor area of 0.39 m ² and Although the height should just be 76.2 cm or more, it is preferable that it is as narrow as possible from the viewpoint of the accuracy and reproducibility of measured values. Specifically, for example, when a cynomolgus monkey is accommodated, a living space inside the test chamber 21 having a length of about 70 cm, a width of about 60 cm, and a height of about 76.5 cm is preferably used.

(2) Structure of carrying cage The monkey used for the measurement is carried from the breeding cage to the chamber device 1 in the carrying cage 100 of the embodiment of the present invention. Hereinafter, a transport cage 100 according to an embodiment of the present invention will be described with reference to the drawings. FIG. 3 is a perspective view of the transport cage 100 according to the embodiment of the present invention. FIG. 4 is a side view of the transport cage 100 according to the embodiment of the present invention. FIG. 5 is a front view of the transport cage 100 according to the embodiment of the present invention. Hereinafter, a transport cage 100 according to an embodiment of the present invention will be described with reference to FIGS. 3 to 5.

The carrying cage 100 according to the embodiment of the present invention is a rectangular parallelepiped, and the front surface 101 has a lattice shape, and the upper surface 102, the bottom surface 103, the side surface 104, and the side surface 105 are made of a metal plate. On the front surface 101 of the transporting cage 100, a door 203 composed of a first door 201 and a second door 202 is installed. The first door 201 and the second door 202 are doors that can be opened and closed with respect to the first opening 300 so that monkeys can enter and leave the second storage portion 800 of the transport cage 100. Specifically, the first door 201 is installed so as to face the first opening 300 through which the monkey enters and exits, and is pulled out in a direction perpendicular to the monkey entry / exit direction at the first opening 300. Is possible. The second door 202 is installed on the opposite side of the first opening 300 with the first door 201 in between, and is detachable from the second housing portion 800. The first door 201 of the transport cage 100 is convenient when moving an animal from the second storage portion 800 of the transport cage 100 to the first storage portion 2 of the chamber device 1. The door 202 can be safely transported so that monkeys do not escape from the second housing portion 800 of the transport cage 100. Both the first door 201 and the second door 202 are preferably made of a metal lattice.

  The second door 202 of the transport cage 100 according to the embodiment of the present invention preferably has a structure of winding up to the upper surface 102 side of the transport cage 100, but may be structured to be pulled up in the vertical direction. As shown in FIG. 4, the drive sprocket 401 is installed on both sides of the upper surface 105 of the transport cage 100 and the driven sprocket is mounted on the upper front surface of the transport cage 100 as shown in FIG. Installed on both sides 402, both sides 403 at the lower back of the transport cage 100, and both sides 404 at the bottom of the front surface 102 of the transport cage 100, wrapped around four sprockets 401, 402, 403 and 404 on one side. Two endless block chains 405 are installed. The block chains 405 on both sides have a large number of metal round bars 406 attached so that adjacent ones are in contact with each other, and the chains on both sides are connected to the four sprockets 401, 402, 403 and 404 on one side. When it is wound, it forms a lattice.

  Further, the drive sprocket 401 installed on the upper surface 102 is provided with a shaft for rotating the drive sprocket 401 and an operation handle 407 for rotating the shaft on the outside of the cage. By providing the operation handle 407 on the outside of the second housing portion 800, the non-human primate housed in the second housing portion 800 can be obtained by joining the first opening 300 and the second opening 7. The first door 201 can be safely rolled up without escaping from the gap. Further, chain stopper plates 408 are installed on both sides of the lower surface of the front surface 101.

  In the bottom surface 103 of the carrying cage 100 according to the embodiment of the present invention, a net-like metal plate that can be inserted and removed as a bottom plate is placed (not shown). The volume of the transport cage 100 can be appropriately selected according to the length of the monkey to be accommodated, but when the monkey is moved from the chamber device 1 to the transport cage 100, the transport cage 100 is placed in the chamber device 1. Therefore, it is preferable to have a size that does not allow a wide gap between the chamber device 1 and the transport cage 100 when inserted.

  Between the front surface 101 and the back surface 105 of the carrying cage 100 of the embodiment of the present invention, a narrow body plate 500 that can be moved back and forth is installed (see FIG. 3). The narrow body plate 500 is moved forward and backward for the purpose of narrowing the monkey housing space of the transport cage 100 from the back surface 105 toward the front surface 101 or vice versa. Therefore, the transport cage 100 according to the embodiment of the present invention can safely move the monkey to the chamber apparatus 1 in a short time without capturing the monkey by the narrow plate 500. The narrow plate 500 is preferably provided with operating handles 501 on both sides (see FIG. 3). The operation handle 501 is preferably installed by being joined by a ball joint or the like so that it can be operated from the outside of the transport cage 100 and can be pulled either forward or backward. When the operator pulls the operation handle 501 with both hands from the outside of the transport cage 100, the narrow plate 500 moves forward and backward. Furthermore, it is preferable that a guide rod penetrating through the narrow plate 500 is horizontally mounted on the back surface and the front surface of the transport cage 100 because the narrow plate 500 can be smoothly advanced and retracted. It is also preferable to have a function such as a stopper so that the monkey cannot easily push back the narrow body plate 500.

The transport cage 100 according to the embodiment of the present invention is provided with a moving caster 600 on the bottom surface 103 thereof. The caster 600 has the purpose of moving the transport cage 100 itself and the purpose of moving the transport cage 100 into the chamber apparatus 1. The caster 600 can be inserted into the rail 61 (see FIG. 2) in the chamber device 1. Moreover, it is also preferable that the carrying cage 100 is placed on a pedestal 700 (see FIG. 4) for eliminating a step with respect to the chamber device 1 so that it can be moved into the chamber device 1 without a step. In this case, if the caster 600 is further attached to the pedestal 700, the transport cage 100 can be moved. Further, when the transport cage 100 is placed on the pedestal 700, it is preferable to install a hook (not shown) for fixing the pedestal 700 and the chamber apparatus 1.

(3) Measuring method using chamber apparatus The chamber apparatus 1 of the embodiment of the present invention is an apparatus that performs measurement for medical and pharmaceutical tests using a monkey housed in the first housing portion 2. However, the use method will be described below by taking as an example the case of conducting a respiratory pharmacological test using monkeys.

  A monkey used for a pharmacological test or the like is transferred from the breeding cage to the transport cage 100 and then transported to the chamber apparatus 1. The procedure for moving the monkey from the transport cage 100 to the chamber apparatus 1 is as follows. First, the second door 42 of the chamber apparatus 1 is opened, the second door 202 of the transport cage 100 is removed, and the second chamber chamber 1 is opened. The opening 7 and the first opening 300 of the transport cage 100 are opposed to each other, and the second opening 7 and the first opening 300 are bonded and held on the bottom surface 103 of the transport cage 100 so as to be bonded. Fix with a hook (not shown). Next, the first door 201 of the transport cage 100 is rolled up and opened, and if the monkey does not move to the first housing part 2 of the chamber device 1, the narrow plate 500 is moved toward the first opening 300. It is possible to move and push the monkey out of the second housing part 800. Therefore, the transport cage 100 according to the embodiment of the present invention can safely move the monkey in a short time without capturing the monkey. After the monkey has moved from the second housing portion 800 to the first housing portion 2 via the first opening portion 800 and the second opening portion 7, the first door 41 of the chamber device 1 is closed and the chamber is fixed with a hook. The connection between the apparatus 1 and the transport cage 100 is released, the second door 42 of the chamber apparatus 1 is closed, and necessary measurements are performed. During this time, the monkey can spend a relatively quiet time by sitting on the protrusion 3 installed in the test chamber 21 as compared to the case without the protrusion 3.

  After the measurement is completed, the procedure for moving the monkey from the chamber apparatus 1 to the transport cage 100 is as follows. First, the second door 42 of the chamber apparatus 1 is opened and the first door 41 is closed. The two openings 7 and the first opening 300 of the transport cage 100 are opposed to each other, and the second opening 7 and the first opening 300 are bonded and held on the bottom surface 103 of the transport cage 100. Secure with an installed hook (not shown). At this time, the first door 201 of the transport cage 100 is left open while the second door 42 of the transport cage 100 is removed. Next, the first door 41 of the chamber apparatus 1 is pulled upward and opened, and the transport cage 100 is inserted into the chamber apparatus 1 to move the monkey to the transport cage 100. After confirming the movement, the first door 201 of the transport cage 100 is closed, and the joint between the chamber device 1 fixed by the hook and the transport cage 100 is released.

  As a method of transferring the monkey from the transport cage 100 to the breeding cage, the second door 202 of the transport cage 100 is removed from the second housing portion 800 and the first door 201 is closed. The breeding cage and the first opening 300 of the transport cage 100 are joined, the first door 201 of the transport cage 100 is opened, and the narrow plate 500 is directed toward the first opening 300 as necessary. It is possible to move and push the monkey out of the second housing part 800. Therefore, the transport cage 100 according to the embodiment of the present invention can safely move the monkey in a short time without capturing the monkey. Alternatively, the first door 201 is opened while the second door 202 is closed, and the narrow plate 500 is pushed toward the first opening 300 to press the monkey so that it cannot move. Thereafter, a catch rod is inserted from the outside of the second door 202 so that the animal's collar cannot be escaped even if the second door 202 is opened. When the second door 202 is opened, both the arms of the animal can be captured by the rear hand, the capture rod can be removed and the animal can be moved back to the breeding cage.

  Hereinafter, the measurement in the chamber apparatus 1 of the Example of this invention is demonstrated in detail. In addition, the measurement in the chamber apparatus 1 is not limited by these. First, the effect of the projection part 3 installed in the chamber apparatus 1 is demonstrated.

  A cynomolgus monkey (3 years old, male) is housed in a first test chamber 2 in which the inner living space is about 70 cm in length, about 60 cm in width, and about 76.5 cm in height in a sealed test chamber 21. The video recording was observed for 3 hours. In addition, an experiment was performed in the same monkey in the same time zone with and without the protrusion 3 being installed on the back surface of the test chamber 21 of the chamber apparatus 1, and the behaviors were compared.

  As a result, in the chamber apparatus 1 in which the protrusions 3 were not installed, the monkeys moved around in the test chamber 21 without being settled, and the search behavior was repeated for about 1 hour. After that, the behavior of sitting in accordance with the chamber apparatus 1 was performed, but it was confirmed that the sitting place is not constant and the place is frequently changed. For example, it has often been confirmed that it comes out to the front surface 52 of the chamber apparatus 1 and looks out or sits at one of the four corners of the chamber apparatus 1.

  On the other hand, in the chamber apparatus 1 provided with the protrusion 3, similarly, the movement first moved around the test chamber 21 and the search behavior was seen, but the time was about 0.5 hours, and it was stored in the chamber apparatus 1 without the protrusion 3. The time for exploring action was shorter than when I did it. In addition, after acclimatization to the chamber apparatus 1, it was confirmed that it often sits on the protruding portion 3 except for exiting to the front surface 52 of the chamber apparatus 1 and looking outside.

(1) Chamber apparatus for respiratory system measurement In this measurement, the chamber apparatus 1 of the Example of this invention was used. The monkey was accommodated in the first accommodating part 2. The test chamber 21 has a length of about 70 cm, a width of about 60 cm, and a height of about 76.5 cm. The chamber apparatus 1 is provided with a respiratory system measuring apparatus described in Japanese Patent Publication No. 2007-500067.

(2) Measurement using monkeys Four cynomolgus monkeys in which physiological saline or morphine hydrochloride 10 mg / kg (manufactured by Takeda Pharmaceutical Co., Ltd., 10 mg / ml) was subcutaneously administered to the chamber apparatus 1 of the example of the present invention (4 Respiratory function was measured by the WHOLE BODY PLETHYSMOGRAPHY method (additional details were added). The monkey administered with physiological saline and the monkey administered with morphine hydrochloride were the same, and the experiment was conducted 1 to 9 days later.

  In addition, the cynomolgus monkey was previously accommodated in the test chamber 21 of the first accommodating portion 2 of the chamber device 1, and the pre-administration value was acquired in a state where the search behavior was calm. Thereafter, the animal was taken out from the chamber device 1, administered with physiological saline or morphine, immediately housed in the chamber device 1, and measured for 6 hours or more. During the measurement time, fasting and free water were used.

  In addition, the respiratory function was measured in the chamber apparatus 1, and at the same time, the cardiovascular system function using the telemetry method, the electrocardiogram, and the central nervous system function using the video camera were observed. Blood pressure was measured as systolic blood pressure, diastolic blood pressure, and mean blood pressure using a data acquisition and real-time analysis system (NOTOCORD-hem 3.5, manufactured by NOTOCORD SYSTEMS) via a receiver using a telemetry method. . The heart rate was measured via a computer system for data acquisition analysis by triggering a pulse wave of blood pressure. The electrocardiogram was measured using a data acquisition / real-time analysis system (NOTOCORD-hem 3.5, manufactured by NOTOCORD SYSTEMS) via a receiver by means of a telemetry method.

Parameters before and after physiological saline and Morphine administration (systolic blood pressure, diastolic blood pressure, mean blood pressure, heart rate and electrocardiogram (PQ interval, QRS group duration, QT interval, QTc), respiratory rate, once The data of ventilation volume, minute ventilation volume, and general symptom observation) were acquired, and the safety pharmacology test core battery IN VIVO test was comprehensively evaluated.

  As a result, in all four cases, the respiratory rate was only slightly decreased by subcutaneous administration of morphine hydrochloride at 10 mg / kg, but the tidal volume and minute ventilation were remarkably decreased. Blood pressure was continuously reduced by the administration of morphine hydrochloride. On the other hand, heart rate and QT interval (QTc) were hardly changed by morphine hydrochloride administration. In general physical condition related to the central nervous system, after morphine hydrochloride administration, inhibitory signs such as decreased locomotor activity, decreased threat to humans, decreased response to humans, drooping eyelids, somnolence were observed continuously. In addition, skin scratching behavior was observed. These behaviors were observed in all 4 cases as well.

  From the above results, it was found that the respiratory function measurement of cynomolgus monkeys by the whole body plethysmography method using the chamber device 1 can be performed without anesthesia and non-restraint, and the results are the same in four cases. The effects on the cardiovascular system and the central nervous system were also observed at the same time.

  As described above, according to the chamber apparatus 1 of the embodiment of the present invention, it is possible to perform measurement in a non-human primate without anesthesia and without restraint with a simple apparatus. About the pharmacological test of respiratory function, it is possible to measure in the anesthesia and unrestrained state using monkeys. The sensitivity to morphine that causes the typical respiratory depression in rats generally used in the above test until now. The problem of low blood flow is also solved, and the fact that dogs can adjust body temperature by breathing and has the property of being easy to vomit may also cause problems that may adversely affect respiratory function measurement. It is possible to perform measurement using animals. Furthermore, since monkeys have similar biological functions to humans, the ability to measure without anesthesia and without restraint can predict the effects on humans.

It is a perspective view of the chamber apparatus of the Example of this invention. It is an AA expanded sectional view of the communicating pipe of the chamber apparatus of the Example of this invention. It is the front perspective view which opened the door of the chamber apparatus of the example of the present invention. It is a schematic perspective view of the carrying cage of the Example of this invention. It is a side view of the conveyance cage of the Example of this invention. It is a front view of the conveyance cage of the Example of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Chamber apparatus 2 ... 1st accommodating part 21 ... Test chamber 22 ... Reference chamber 23 ... 1st airflow opening 24 ... 2nd airflow opening 25 ... External air Flow opening 26 ... Communication pipe 27 ... Separation wall 3 ... Projection part 4 ... Door part 41 ... First door 42 ... Second door 51 ... Upper surface 52 ... -Front surface 53 ... Side surface 54 ... Back surface 55 ... Bottom surface 6 ... Reticulated metal plate 61 ... Rail 7 ... Second opening 8 ... Feeding box 9 ... Water supply device 100 ... Carrying cage 101 ... Front face 102 ... Top face 103 ... Bottom face 104 ... Side face 105 ... Back face 203 ... Door part 201 ... First door 202 ... Second Door 300 ... first opening
401 ... Drive sprockets 402, 403, 404 ... Driven sprocket 405 ... Block chain 406 ... Metal round bar 407 ... Handle for operation 408 ... Stopper plate 500 ... Narrow body plate 501 ... Operation handle 600 ... Caster 700 ... Pedestal 800 ... Second housing

Claims (6)

A non-anesthetized, non-restrained measuring chamber device for non-human primates,
A containment unit containing non-human primates;
A protrusion capable of seating a non-human primate on the inner side of the housing;
An opening for a non-human primate to enter and exit the housing from outside,
A door part that is installed outside the opening part and adjusts the access to the accommodating part of the non-human primate through the opening part by opening and closing ; and
The accommodating portion is surrounded by a transparent wall and an opaque wall constituting the wall of the measurement chamber device,
The door part is
A first door installed so as to face the opening, and capable of being pulled out in a direction perpendicular to a direction in which the non-human primate enters and exits the opening;
A second door that is installed on the opposite side of the opening across the first door, and that can block air from entering and exiting the housing through the opening, and
Chamber device. In the opening, when the second door is opened, a transport cage for transporting the non-human primate can be joined.
The chamber apparatus according to claim 1. The second door is detachable with respect to the housing portion,
When the chamber device is connected to an enclosure that prevents non-human primates from escaping, and the non-human primate is transferred from the enclosure to the chamber device, the second door is removed from the housing portion. The first door is withdrawn in a direction perpendicular to the access direction of the non-human primate,
The chamber apparatus of Claim 1 or 2. Between the first door and the second door, accessories necessary for housing non-human primates are installed,
The chamber apparatus of Claim 2 or 3. The chamber apparatus according to any one of claims 1 to 4,
A chamber apparatus for performing a pharmacological test of a non-human primate respiratory system,
The accommodating portion is
A laboratory where pharmacological tests are carried out;
A reference chamber for comparison with a pharmacological test in the laboratory, arranged in parallel with the laboratory,
The test chamber and the reference chamber are communicated with each other by a communication pipe that supplies outside air.
Chamber device. The non-human primate is a monkey,
The chamber apparatus as described in any one of Claim 1 to 5.

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