JP5117168B2 - Oral skin improving agent and skin improving food - Google Patents

Description

  The present invention relates to a skin improving agent and a skin improving food, and more particularly to a skin improving agent and a skin improving food for improving a minute skin state that changes every day.

  Conventionally, collagen, hyaluronic acid, etc. are typical health foods for beautifying the skin, but these are ingredients that are inherently present in the skin, and by taking these, the shortage is compensated for, It is to improve. However, since all of these have a large molecular structure, it is considered difficult for all absorbed in the body to reach the skin as it is and to work directly as a constituent of the skin.

  On the other hand, substances that are not constituents of the skin but lead to beautiful skin when taken orally are known in the art, such as ascorbic acid, soybean isoflavone, cysteine, astaxanthin, tocopherol, tocotrienol and the like. In particular, ascorbic acid, tocopherol and soy bean, for example, to complement each other's efficacy effect by combining these, or synergistically enhance the effect to obtain a skin-beautifying effect such as a whitening effect or a rough skin-improving effect. A combination of isoflavones (Patent Document 1), a combination of proanthocyanidins, ascorbic acid and cysteine (Patent Document 2), a combination of astaxanthin and tocotrienol (Patent Document 3), and a combination of ascorbic acid and pearls (Patent Document 4). .

  By the way, in the trypsin degradation product of casein, the decapeptide described in Patent Document 5 is known, and this decapeptide purified product has been confirmed to have anticonvulsant and sedative effects in a test using rats. Yes. Patent Document 6 describes that there is an effect of reducing pet stress. Further, according to Non-Patent Documents 1 and 2, it is confirmed that the relaxant action, sedative action, antistress action using rats, and the effectiveness of stress factors against sleep disorders are confirmed. However, the effect on the skin by oral intake of decapeptide has never been known.

On the other hand, GABA is an abbreviation for γ-amino butyric acid, which is a kind of amino acid widely distributed in animals and plants and the like, and is an inhibitory neurotransmitter present in the brain and spinal cord of mammals. GABA has the function of improving cerebral blood flow, increasing oxygen supply, and renewing cerebral metabolism, so it can be applied to the treatment of headache, tinnitus, decreased desire, etc. . As other physiological effects, improvement of learning ability and activation of renal function are known. Furthermore, according to Non-Patent Document 3, GABA has been shown to be related to relaxation, but no skin beautifying effect in combination with different components has been known.
JP 2005-229855 A JP 2005-323604 A JP 2003-325135 A JP 2003-335668 A Japanese Patent No. 397244 JP 2004-357504 A Health Industry Newspaper No. 1150 (May 10, 2006) Food and Containers Vol.46 No. (10) P555-559 (2005) Neurochem Res. Vol.28, No.6, p965-976, 2003

  As a result of extensive studies by the present inventor, it was confirmed that a skin improvement effect was exhibited by combining the decapeptide and GABA or GABA derivative. That is, the present invention aims to provide a skin improving agent and a skin improving food having extremely high efficacy by combining a decapeptide and GABA or a GABA derivative, which have not been known to have a skin improving effect. It is.

The skin-improving agent of the present invention contains a decapeptide described in SEQ ID NO: 1 in the sequence listing and GABA or a GABA derivative, that is, a decapeptide and GABA described in SEQ ID NO: 1 in the sequence listing, or a sequence number in the sequence listing. The decapeptide according to 1, and a GABA derivative are contained.
The decapeptide is preferably an enzyme degradation product of casein.

  The skin-improving food of the present invention comprises a decapeptide and GABA described in SEQ ID NO: 1 in the sequence listing, or a decapeptide and GABA derivative described in SEQ ID NO: 1 in the sequence listing. The decapeptide is preferably an enzyme degradation product of casein.

  By orally ingesting the skin-improving agent or food for improving skin according to the present invention, the effect of improving the skin condition and the effect of preventing deterioration can be obtained. Specifically, skin gloss, pore conspicuousness, bulkiness, moist feeling, refreshing feeling, stickiness, fine lines, dark circles in the eyes, transparency, greasy, acne, pimples, skin firmness, skin texture, An improvement effect can be obtained with respect to makeup glue, makeup loss, and the like.

  The skin-improving agent of the present invention contains a decapeptide described in SEQ ID NO: 1 in the sequence listing and GABA or a GABA derivative.

  The decapeptide described in SEQ ID NO: 1 in the sequence listing is preferably an enzyme degradation product of casein. Casein accounts for about 80% of the milk protein contained in milk and is one of the main components of the component generally called milk solids. Its constituent components are not a single protein, but are divided into main fractions of γ-casein, κ-casein, β-casein, αs1-casein and αs2-casein. To obtain the decapeptide in the present invention, More preferably, αs1-casein is used. αs1-casein can be obtained from milk, whole caseins, and acid caseins by a conventional method. For example, a method of fractionating with DEAE-cellulose using a calcium chloride concentration gradient as a solubilizer can be exemplified. it can.

  The decapeptide described in SEQ ID NO: 1 in the sequence listing is preferably obtained from αs1-casein by enzymatic degradation of αs1-casein, preferably by hydrolysis with an enzyme using trypsin. This can be obtained from milk casein.

  In the case of an enzymatic degradation product of casein, a peptide containing a decapeptide described in SEQ ID NO: 1 in the sequence listing, that is, a peptide having a slightly longer amino acid sequence than a decapeptide described in SEQ ID NO: 1 in the sequence listing, Although it may contain an oligopeptide in which one or more amino acids are deleted from the sequence of the decapeptide described in SEQ ID NO: 1 in the sequence listing, it may be purified and used only for the decapeptide described in SEQ ID NO: 1 in the sequence listing. It is also possible to use them as an entire enzymatic degradation product of casein without particularly removing them.

  GABA in the present invention can be obtained by fermentation using germinated rice, green tea, rice bran lactic acid bacteria, or fermenting glutamic acid using lactic acid bacteria, but is not particularly limited thereto. Further, derivatives of GABA are also known, and for example, N-methyl-γ-aminobutyric acid, N-dimethyl-γ-aminobutyric acid or esters thereof, for example, γ-aminooleyl ester can be suitably used.

  In the skin improving agent of the present invention, the component ratio of decapeptide and GABA (or GABA derivative) described in SEQ ID NO: 1 in the sequence listing is used as a skin improving agent, a skin improving food, Since it differs depending on the purification state, etc., it cannot be generally stated, but when it is blended as a casein enzymatic degradation product, the ratio of the casein enzymatic degradation product to GABA is generally 50 to 1: 1 to 50, preferably 15 to 1: 1 to 8 Further, it is preferably about 5 to 3: 1 to 2.

  The daily intake of an average adult (about 60 kg) of the skin improving agent of the present invention varies depending on the mixing ratio of the decapeptide and GABA (or GABA derivative) contained, the purification state of the decapeptide, etc. Therefore, although it cannot be generally stated, when the decapeptide is formulated as a casein enzyme degradation product, the amount is preferably 100 mg / day or more, more preferably 200 mg / day or more in terms of decapeptide dry matter.

  In the case of skin-improving foods, unlike the skin-improving agents, priority may be given to the original taste, texture, mouth-melting, etc. of the food and shape retention as a food, and the decapeptide and GABA (or GABA derivative) Since the component ratio and the daily intake are greatly affected by this, in this case, the component ratio and the intake range in the above-described skin improving agent are not necessarily required.

  The dosage form of the skin improving agent of the present invention is not particularly limited, and for example, solid preparations such as tablets, powders, fine granules, granules, hard capsules, soft capsules, pills, liquid preparations, suspensions And liquids such as syrups and emulsions. The skin-improving agent of the present invention includes excipients, disintegrations, bonds, lubricants, surfactants, alcohols, water, water-soluble polymers, sweeteners, corrigents, acidulants, drugs that are usually used depending on the form. It can be produced by a commonly used method by adding a carrier for use.

  In skin-improving foods, confectionery such as candy, cookies and biscuits, fish paste products such as kamaboko and chikuwa, livestock products such as sausage and ham, raw noodles, noodles such as boiled noodles, sauces, soy sauce and sauce It can be used as a general food and drink such as seasonings, pickles, prepared dishes, and soft drinks. At that time, the skin-improving food of the present invention is a variety of components usually used depending on the food, such as sugar, flour, shortening, salt, glucose, chicken egg, butter, margarine, chickenpox, calcium, iron, seasoning, spice Etc. can be added, and it can manufacture by the method normally used.

  Examples of the present invention are shown below. In addition, although the decapeptide used in the following Example uses what purified the enzyme degradation product based on Example 1 of patent document 5, you may use the decapeptide marketed.

Example 1
Hard capsule Decapeptide 54% (wt%)
GABA 36%
Crystalline cellulose 6%
Calcium phosphate 2%
Sucrose fatty acid ester 2%
The contents of the above formulation were added to a hard capsule (pig gelatin transparent No. 2) to produce a hard capsule skin improver.

(Comparative Example 1)
A hard capsule skin-improving agent was prepared in the same manner as in Example 1 except that theanine was used instead of decapeptide and GABA in Example 1.

(Comparative Example 2)
A hard capsule skin-improving agent was prepared in the same manner as in Example 1 except that GABA was used instead of decapeptide in Example 1 (all except that GABA was used).

<Skin improvement test>
For the three types of hard capsules of Example 1 to Comparative Example 2, for 10 subjects (total of 30 women in their 20s and 40s), decapeptide 150 mg / day hard capsules in Example 1 and Comparative Example 1 Theanine 200 mg / day hard capsules, and in Comparative Example 2, GABA 100 mg / day hard capsules were orally administered for 1 week, the amount of sebum and pH of the cheeks were measured, and a questionnaire regarding skin condition was conducted. The amount of sebum was measured using a cebu meter (Courage + Khazaka Electronic GmbH), and the pH was measured using a pH meter. Changes in sebum amount and pH are shown in Tables 1 and 2, respectively, and the questionnaire results of the skin condition are shown in FIGS. In addition, the dose of each substance per day was set to the amount in which the effect was recognized with reference to the material manufacturer information and literature etc. which investigated the relaxation effect, the sleep improvement effect, etc.

  As is apparent from FIGS. 1 to 3, when “slightly improved” is included in the recognized effects, 80% of the hard capsules of Example 1 administered orally were glossy on the skin, dark circles on the eyes, and transparency. The results showed that improvement in makeup paste and makeup breakage was observed, 70% improved in conspicuous pores, and 60% was effective in greasy and acne. Considering that the administration of this hard capsule is as short as one week and that the period of skin rebirth is generally 28 days (so-called turnover), this represents a very high skin improvement. Compared with Comparative Example 1 mainly composed of theanine, which is said to have a relaxing effect like GABA, and Comparative Example 2 mainly composed of GABA, the effect is almost doubled.

  Also, in this skin improvement test, the effect on bulkiness, fine lines, and skin elasticity was less than half of 40%, but the bulkiness, fine lines, and skin tension improved daily by turnover. Therefore, it can be predicted that continuous intake will produce further effects.

  Furthermore, from a numerical point of view, the amount of sebum was slightly increased for the hard capsules of Example 1 as shown in Table 1, but the individual differences were large and not a remarkable result, but as shown in Table 2. It was observed that the pH decreased in a slightly acidic direction, which was considered ideal.

Below, the formulation example of another formulation and the formulation example of a foodstuff are shown.
(Example 2) Tablet Decapeptide 30.0 (wt%)
GABA 5.0
Magnesium stearate 0.005
Lactose Appropriate amount Cellulose Appropriate amount After mixing uniformly with the above formulation (adjusted to 100% by weight with lactose and cellulose), the mixture was tableted with a tableting machine to give a tablet with a diameter of 10 mm and a weight of 300 mg.

(Example 3) Powder Decapeptide 17.0 (wt%)
GABA 17.0
Reduced lactose 35.0
Soybean oligosaccharide 3.0
Erythritol 3.0
Dextrin 25.0
Purified water appropriate amount All of the above formulation was uniformly mixed and stirred and spray-dried to obtain a powder.

(Example 4) Soft capsule Decapeptide 20.0 (wt%)
GABA 10.0
Soybean oil 70.0
All of the above formulation was mixed and stirred uniformly, and filled into a soft capsule skin (oval type, weight 150 mg) by a conventional method to obtain a soft capsule.

(Example 5) Beverage Purified water 87.1 (% by weight)
Decapeptide 2.5
GABA 1.0
Fructose 9.0
Citric acid 0.3
Fragrance 0.1
With the above formulation, everything was uniformly mixed and stirred to obtain a beverage.

(Example 6) Candy White sugar 60.5 (% by weight)
Mizuame 38.9
Decapeptide 0.2
GABA 0.3
Fragrance 0.1
Sucrose and syrup were mixed by heating and homogenized, cooled, added decapeptide, GABA and fragrance at 70 ° C., mixed and homogenized, and molded.

(Example 7) Cookies Soft flour 45.0 (% by weight)
Butter 17.5
Granulated sugar 20.0
Decapeptide 4.0
GABA 1.0
Egg 12.5
Fragrance Appropriate amount While stirring butter, granulated sugar was gradually added, and then egg, decapeptide, GABA and flavor were added and further stirred. After mixing well, the weakly shaken flour was added, stirred at a low speed and laid in a refrigerator in a lump. Thereafter, it was molded and baked at 170 ° C. for 15 minutes to obtain a cookie.

Questionnaire results one week after ingestion of hard capsules of Example 1 Questionnaire results one week after ingestion of hard capsules of Comparative Example 1 Questionnaire results 1 week after ingestion of hard capsules of Comparative Example 2

Claims (3)

A decapeptide described in SEQ ID NO: 1 in the sequence listing;
Improves bear, greasy or acne , characterized by containing any of GABA, N-methyl-γ-aminobutyric acid, N-dimethyl-γ-aminobutyric acid and γ-aminooleyl ester Oral skin improvement agent. The oral skin improving agent according to claim 1, wherein the decapeptide is an enzyme degradation product of casein. A skin improving food comprising the oral skin improving agent according to claim 1 or 2.

Images