JP5066072B2 - Liquid chromatograph - Google Patents

Description

  The present invention relates to a liquid chromatograph apparatus, and more particularly to an analysis function when there is a problem with an analysis result.

  In recent years, advanced functions of chromatographic data processing apparatuses have progressed, and their operation methods have become complicated. Therefore, in the conventional operation screen, taking into account the convenience of the analyst, the frequently used functions are picked up, arranged with operation menus and icons, etc., and the operation menu of each function is hierarchized, etc. Simplification. Some have a function to navigate the operation procedure, improve the operability, and have an analyzer / analyzer interface that prevents operation errors.

Japanese Patent Laid-Open No. 5-322870 JP 2007-40905 A

  In recent years, it has become possible to perform a large amount of analysis in a short time using a high-pressure, high-flow rate liquid chromatograph apparatus. Therefore, it is necessary to determine whether or not a large amount of analysis results are correct, and when a measurement result or analysis result that deviates from the expected value is obtained, work for identifying the cause is necessary. However, this operation is very difficult in the following points.

  In recent years, in the analysis work in the chromatographic apparatus, the division of labor is different for each work. For example, a person who defines a measurement method, a person who analyzes and measures according to an analysis measurement method, a person who performs data analysis, and a person who summarizes a report are different from each other. Therefore, there is a case where the person in charge of analyzing the sample does not have sufficient knowledge about the analysis of the problem and asks the higher-order analyst or the specialist in charge for analysis. In this case, the person in charge may not be able to determine what information should be extracted and reported. Also, if the amount of data is large, it is difficult to extract target information from the data amount.

  In particular, when the measured value or analysis result is slightly different from the expected value, there is a possibility that the calculation accuracy, processing procedure, and judgment conditions in the data processing device are not a problem, but a problem with the operation or analysis device. is there. In such a case, more detailed information and calculation progress information are required for the analysis than the analysis result that is normally output.

  An object of the present invention is to provide means for easily identifying a problem when a measured value or an analysis result deviates from an expected value.

  According to the liquid chromatograph apparatus of the present invention, the threshold value setting screen or the operation history confirmation screen is displayed on the display device.

  The threshold setting screen includes an input field for inputting a threshold value for the accuracy of the measurement value obtained by the chromatographic analysis, and a specification field for specifying information to be displayed on the operation history confirmation screen when the accuracy of the measurement value is lower than the threshold value. Have.

  The operation history confirmation screen is specified in the operation history executed until obtaining the analysis result of the chromatograph, the threshold value of the accuracy of the measurement value input in the input field of the threshold setting screen, and the specification field of the threshold setting screen Displayed information.

  According to the present invention, when the identified analysis result deviates from the expected value, the problem can be easily identified.

  Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 shows a configuration example of a standard liquid chromatograph apparatus including a chromatographic data processing apparatus according to the present embodiment. The liquid chromatograph apparatus includes a plurality of types of eluents 101, a pump 102, an autosampler 103, a column oven 104, a detector 105, and a data processing device 106. The column oven 104 has a separation column and an oven. The data processing device 106 includes a storage device 107, to which a display 108, a keyboard 109, a mouse 110, and a printer 111 are connected.

  A plurality of types of eluents 101 are each sucked by the pump 102 and sent to the autosampler 103. In the autosampler 103, a sample is injected into the eluent. The sample is sent to the column oven 104 together with the eluent. In the column oven 104, each component contained in the sample is separated by a separation column maintained at a constant temperature by the oven. Each separated component is detected by the detector 105 and discarded in a waste liquid container. The chromatograph obtained by the detector 105 is sent to the data processing device 106. The data processing device 106 calculates the height and area of the chromatopeak, stores the measured values of each component in the storage device 107, and outputs them to the display 108 and the printer 111. The analysis measurer inputs analysis conditions and sample settings via an input device such as the keyboard 109 or the mouse 110. Various input screens, operation screens, and output screens are displayed on the display 108. Further, the data processor 106 controls the pump 102 to adjust the flow rate of the eluent 101, controls the autosampler 103, adjusts the sample injection amount, controls the column oven 104, and controls the temperature of the oven. Adjust.

  In the analysis using the liquid chromatograph apparatus, the analysis result or the measurement result may be different from the expected value. In such a case, it is necessary to identify the cause. According to the invention, the analysis result or measurement result is first compared with the expected value. When the deviation between the two is large, the operation history is read. In this way, the cause can be investigated from the operation history. In the analysis using a liquid chromatograph apparatus, various measurement values are obtained as analysis results or measurement results. Such measured values include peak identification values, statistical values, system suitability test (SST) values, and the like. However, in the following example, a calibration curve will be described as a measured value. That is, the case where the accuracy of the calibration curve is different from the expected value will be described.

  The calibration curve will be described with reference to FIG. In the liquid chromatograph apparatus, a chromatograph in which the vertical axis represents signal intensity and the horizontal axis represents time is obtained by the output from the detector 105. The relationship between time and components is known. Therefore, the time on the horizontal axis indicates the type of component contained in the sample. The area of the chromatographic peak represents the concentration of each component. A calibration curve is required to know the concentration of each component from the peak area.

  A calibration curve is prepared using a standard sample. First, a plurality of standard samples having different concentrations are prepared. The concentration of the components contained in the standard sample is known. Next, liquid chromatographic measurements are performed on these standard samples to obtain chromatographs. The peak area of each component is measured from the chromatograph. Since the concentration of each component is known, the relationship between the concentration and the peak area can be obtained. This is a calibration curve. FIG. 7 shows the coordinates of the concentration on the horizontal axis and the peak area on the vertical axis. On this coordinate, the measurement result of the liquid chromatography measured for the standard sample is plotted. The peak area is proportional to the concentration. If the liquid chromatograph measured on the standard sample is accurate, the calibration curve should be a straight line. A straight line passing through the plotted points is a calibration curve. The calibration curve is expressed by the following equation (1).

  Here, xi is the concentration of each component of the standard sample, and yi is the measured value (area value) of each component of the standard sample. The first expression of Equation 1 is a linear expression including the slope a and the intercept b. The slope a and the intercept b are obtained by the method of least squares as shown in the second and third expressions.

  If the measurement result is accurate for the standard sample, the relationship between the concentration and the peak area should be on a straight line, as indicated by point 702 in FIG. However, the relationship between the concentration and the peak area may not be on a straight line due to various factors. For example, points 703 and 704 in FIG. 7 show another example in which the measurement result of the liquid chromatography measured for the standard sample is plotted. Even in such a case, the calibration curve is obtained as a straight line by the equation (1).

  A straight line 701 in FIG. 7 indicates a calibration curve calculated from the measurement result using the standard sample. When the measurement result of the standard sample is greatly deviated from the calibration curve as indicated by points 703 and 704 in FIG. 7, the accuracy of the calibration curve may not be high. Here, the degree of coincidence is used as a parameter that quantitatively indicates the accuracy of the calibration curve. The degree of coincidence is obtained by the following equation (2).

  Here, fxi is the value of the Y coordinate of the point on the calibration curve corresponding to the concentration of each component of the standard sample. yavg is an average of measured values (area values) of each component of the standard sample. The degree of coincidence takes a positive value of 1 or less. It can be said that the closer the degree of coincidence is to 1, the smaller the deviation of the measured value of the standard sample from the calibration curve. That is, it can be said that the accuracy of the calibration curve is higher as the coincidence degree is closer to 1.

  With reference to FIG. 2, the analysis operator's work and the flow of analysis and information acquisition (analysis scenario) will be described. In this example, the matching degree of the calibration curve is calculated, and if the matching degree of the calibration curve is low, processing necessary to investigate the cause is performed. In step S201, the analysis measurer sets analysis conditions. The analysis conditions may be set via an analysis condition setting screen (not shown) displayed on the display 108. Analysis conditions include apparatus conditions and processing conditions. The apparatus conditions are set conditions for the pump 102, the autosampler 103, the column oven 104, and the detector 105. For example, the setting conditions of the autosampler include a sample injection amount and the like. The setting conditions of the column oven include the temperature setting of the temperature-controlled room. The processing conditions are the chromatographic processing conditions in the data processing device 106. For example, setting a baseline when measuring the area of the peak. In step S202, the analysis measurer sets sample conditions. The sample conditions may be set via a sample condition setting screen (not shown) displayed on the display 108. Sample conditions include sample vial, concentration, and the like. In step S203, the analysis measurer sets a threshold value. That is, the threshold value of the calibration curve is set. The calibration curve coincidence is a positive value of 1 or less. Therefore, the threshold value is a positive value smaller than 1.

  In step S204, the analytical measurer performs liquid chromatographic measurement on a standard sample whose component concentration is known to obtain a chromatograph. In step S205, secondary processing of the measured value is performed. That is, the measured value is smoothed. The smoothing process includes a chromatographic smoothing process. In step S206, the coincidence of the calibration curves is calculated. The degree of coincidence is calculated according to the equation (2). In step S207, the matching degree of the calibration curve is compared with a threshold value. If the coincidence of the calibration curves is smaller than the threshold value, it is determined that the accuracy of the calibration curves is low, and the process proceeds to step S208. When the matching degree of the calibration curve is equal to or greater than the threshold value, it is determined that the accuracy of the calibration curve is high, and the process proceeds to step S210.

  In step S208, the operation history confirmation screen shown in FIG. 6 is displayed. On this operation history confirmation screen, the operation history until the measurement value is obtained is displayed. An example of the operation history confirmation screen in FIG. 6 will be described later. In step S209, the analysis measurer reviews the operation history on the operation history confirmation screen, and examines the cause of the error in the measurement result that is the basis for creating the calibration curve in the operation. The analytical measurer reports the cause of the error to the superior analyst. In step S210, the analysis result is output to an external device.

  FIG. 3 shows an example of the threshold setting screen. This threshold value setting screen is displayed in step S203 of FIG. In this threshold setting screen, a column 301 for inputting a threshold value of the calibration curve matching level and a column 302 for specifying information to be output when the matching level of the calibration curve is smaller than the threshold value are displayed. In the example shown in the figure, “0.98” is input as the threshold value of the matching degree of the calibration curve. The information output when the coincidence of the calibration curves is smaller than the threshold value is information necessary for investigating the cause. According to this example, the operation history is always displayed. Therefore, it is possible to specify an item that the analysis measurer determines to be necessary in items other than the operation history. In the example shown in the figure, the output information includes analysis conditions (apparatus conditions, processing conditions), vials, concentrations, baseline measurement results, top peak values, valley values, area values, chromatograms, calibration curve data, etc. Yes, a check is entered in those check boxes 302. When a check is entered in the check box and the setting button 303 is clicked, the setting contents are stored as a problem analysis scenario. The setter can control the amount of information to be output by selecting an item to be checked.

  In this example, an operation history is displayed. Thereby, it is possible to know the history of the operation of the analysis / measurement person. By displaying the analysis conditions (apparatus conditions, processing conditions), vials, and concentration, it is possible to determine whether or not there has been an input error or the like in the operation of the set values of the analysis conditions and sample conditions. By displaying the baseline measurement result, the top peak value, the valley value, the area value, and the chromatogram, it is possible to obtain information on whether or not there is a problem in the accuracy of the measuring instrument, the quality of the obtained data, the shape, and the like. By displaying the data at the time of creating the calibration curve, it is possible to obtain information on the accuracy of the calibration curve.

  FIG. 6 shows an example of the operation history confirmation screen. This operation history confirmation screen is displayed in step S208 of FIG. The operation history confirmation screen in this example includes an area 601 for displaying an operation history, an area 602 for displaying a chromatograph, an area 603 for displaying baseline measurement results, peaks, valleys, and peak areas, and analysis conditions (apparatus conditions, processing). Area 604 for displaying (condition), an area 605 for displaying the concentration, and an area 606 for displaying the calibration curve data. In an area 601, an operation history by the analysis / measurement person is displayed. The analysis measurer can know the operation history by the analysis measurer by reviewing the operation history of the area 601. Details of the area 601 are shown in FIG. In the areas 602, 603, 604, 605, and 606, items set in the check box 302 of the threshold setting screen in FIG. 3 are displayed.

  In the area 602, one of a plurality of chromatographs is automatically selected and displayed. Here, it is possible to display a plurality of chromatographs simultaneously or by overwriting. Therefore, the analytical measurer can confirm whether or not the measurement value is abnormal by observing the chromatograph displayed in the area 602. In the area 603, the baseline measurement result, peak, valley, and peak area are displayed for the chromatograph displayed in the area 602. Here, these plural data are displayed in a lump. Therefore, the analytical measurer can confirm whether there is any abnormality in the baseline measurement result or the like.

  In the area 604, analysis conditions (apparatus conditions and processing conditions) are displayed for the chromatograph displayed in the area 602. The analysis measurer can confirm whether there is a problem in setting the analysis conditions. In the area 605, measurement information such as the vial position, concentration, and number of repeated measurements is displayed for the chromatograph displayed in the area 602. The analytical measurer can confirm whether there is an error in the designation of the sample. In area 606, an internal calculation value used for calculation of the calibration curve is displayed.

  FIG. 4 shows an operation history display area which is a part of the operation history confirmation screen. This screen is an enlargement of the area 601 of the operation history confirmation screen of FIG. Only this operation history display area may be displayed on the full screen. In the operation history display area of this example, a column area displaying the operation procedure number 401, a column area displaying the operation procedure screen 405, a column area displaying the operation content 406 of the operation procedure, and a comment 407 for the operation procedure are input. A column area to be displayed, and a column area 408 for displaying an execution button for editing such as deletion of an operation procedure.

  An operation procedure number 401, an operation procedure screen 405, an operation procedure operation content 406, and a comment 407 are displayed in the corresponding row area for each operation procedure number.

  The operation procedure number 401 indicates the order of operations performed by the analyst. The operation procedure screen 405 displays an operation screen used for the operation performed by the analyst. In this example, since all the operation screens used for the operation are displayed, screen transition can be grasped.

  The operation content 406 displays the content operated by the analysis / measurement person. In particular, analysis conditions (apparatus conditions, processing conditions) changed on the screen are shown. For example, operation procedure number 10 indicates that the analyst has displayed the waveform screen and has pressed the smoothing button on the waveform screen. Further, it is shown that the analysis measurer sets “0.98” as the threshold value of the matching degree of the calibration curve. In operation procedure number 11, the analysis measurer displays the smoothing setting screen, sets the processing method, the smoothing order, the number of data points, the number of times, and the like, and displays that the OK button has been pressed. In operation procedure number 12, it is indicated that the analysis / measurement person displays the waveform screen again and presses the smoothing button on the waveform screen. In operation procedure number 13, the analysis measurer displays the smoothing setting screen, changes the smoothing order and the number of data points, and displays that the OK button is pressed. Here, the processing method and the number of times are not displayed because they are not changed. In the operation procedure number 14, it is shown that the analysis / measurement person has displayed the waveform screen again.

  In the example shown in the figure, it can be seen that the analysis / measurement person manually performs the smoothing process of step S205 of FIG. 2 twice. Note that when the analysis / measurement person reports the execution contents of the smoothing process to the higher-order analyst, it is only necessary to report only the last executed process indicated by the operation procedure number 13. Therefore, a delete button is provided as means for deleting redundant information. A comment 407 indicates a comment input or changed by the analysis / measurement person. An analysis measurer can supplement the comment with the operation.

  When the analysis measurer presses the save button 402, the contents displayed in the operation history display area are saved. When the analysis / measurement person presses the print button 403, the operation history display area is printed. When the analysis measurer presses the end button 404, the operation history display area is closed.

  FIG. 5 shows an example of the operation history saving screen. When the save button 402 is clicked on the screen on which the operation history display area of FIG. 4 is displayed, the operation history save screen of FIG. 5 is displayed. The operation history saving screen of this example includes a text box 501 for setting the file name of the operation history, a text box 502 for setting the description of the operation history, and a button 503 for saving the operation history file. The analysis measurer inputs the operation history file name in the text box 501, inputs the description of the operation history in the text box 502, and clicks the button 503. The information shown on the operation history confirmation screen in FIG. Filed.

  According to this example, the information shown in the operation history display area of FIG. 4 is automatically collected and output collectively according to the analysis scenario procedure. Therefore, the analytical measurer can easily identify the problem. In addition, it is possible to easily perform operations such as reporting from an analysis measurer to a higher-order analyst, and requesting analysis. In addition, by setting an analysis scenario in advance, it is possible to acquire necessary information for analyzing the problem regardless of the experience of the analyst.

  This information can be printed or stored in a storage medium. The information can be stored as a database, and the same information as a problem that has occurred previously can be searched and acquired from the database. For example, as an example of information search, the same analysis data can be searched using the case where the matching degree of the calibration curve is 0.95-0.98, the case where the matching degree of the calibration curve is 0.80 or less, and the like as keywords.

  In the above example, the calibration curve coincidence is described as an example. However, the present invention is also applicable when the peak identification value, statistical value, system suitability test (SST) value, etc. are more deviated than expected. In this case, as an internal calculation value, an analysis scenario of a value in accordance with an expected value such as a statistical value or a peak half width is selected, and analysis information is output.

  According to the present invention, when the specified analysis result deviates from the expected value, the data processing apparatus automatically collects and outputs information necessary for analyzing the problem according to the scenario information designated in advance. Therefore, there is an effect that the problem can be easily identified.

  As mentioned above, although the example of this invention was demonstrated, it will be easily understood by those skilled in the art that the present invention can be variously modified within the scope of the invention described in the claims.

It is a figure which shows the structural example of the na liquid chromatograph apparatus by this invention. It is a figure explaining the flow of an analysis measurer's operation | work, analysis, and information acquisition in the liquid chromatograph apparatus by this invention. It is a figure which shows the example of a threshold value setting screen in the liquid chromatograph apparatus by this invention. It is a figure which shows a part of example of the operation log confirmation screen in the liquid chromatograph apparatus by this invention. It is a figure which shows the example of an operation history preservation | save screen in the liquid chromatograph apparatus by this invention. It is a figure which shows the example of the operation log confirmation screen in the liquid chromatograph apparatus by this invention. It is a figure explaining the coincidence degree of the calibration curve and the calibration curve in the liquid chromatograph apparatus by this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 101 ... Eluent, 102 ... Pump, 103 ... Autosampler, 104 ... Column oven, 105 ... Detector, 106 ... Data processing device, 107 ... Memory | storage device, 108 ... Display, 109 ... Keyboard, 110 ... Mouse, 111 ... Printer

Claims (14)

A pump that sucks the eluent, an autosampler that injects the sample into the eluent, a column oven that separates the sample into components, a detector that detects each separated component, and a detector obtained through the detector. In a liquid chromatograph apparatus comprising a data processing device for calculating the concentration of each component from the chromatograph, and an input device and a display device connected to the data processing device,
The display device displays a threshold setting screen or an operation history confirmation screen according to a command from the input device,
The threshold setting screen designates an input field for inputting a threshold of accuracy of a measurement value obtained by analysis of a chromatograph, and information displayed on the operation history confirmation screen when the accuracy of the measurement value is lower than the threshold Has a designation field to
The operation history confirmation screen is an operation history in the data processing apparatus executed until obtaining the analysis result of the chromatograph, a threshold value of the accuracy of the measurement value input in the input field of the threshold setting screen, The liquid chromatograph apparatus characterized by displaying information designated in a designation column of the threshold setting screen. The liquid chromatograph apparatus according to claim 1,
The operation history displayed on the operation history confirmation screen includes an operation content executed by the data processing device and an operation screen displayed on the display device when the operation content is executed. A liquid chromatograph apparatus. The liquid chromatograph apparatus according to claim 1,
The operation history confirmation screen includes an operation history display area for displaying the operation history. The operation history display area includes an area for displaying an operation procedure number, an area for displaying an operation procedure screen, and an operation procedure operation. A liquid chromatograph apparatus including an area for displaying contents, a comment for an operation procedure, or a button for editing an area for display and an operation procedure. The liquid chromatograph apparatus according to claim 1,
The display device displays an operation history storage screen according to an instruction from the input device, and the operation history storage screen has a field for inputting a file name, and the file name is input via the input device. And a liquid chromatograph apparatus in which the operation history displayed on the operation history confirmation screen is stored as a file. The liquid chromatograph apparatus according to claim 1,
The threshold setting screen has an input field for inputting a threshold value for the calibration curve matching level, and a designation field for designating information to be displayed on the operation history confirmation screen when the matching level for the calibration curve is smaller than the threshold value. ,
The degree of coincidence of the calibration curves is determined by the following equation.

Here, xi is the concentration of each component of the standard sample, and yi is the measured value (area value) of each component of the standard sample. fxi is the value of the Y coordinate of the point on the calibration curve corresponding to the concentration of each component of the standard sample. yavg is an average of measured values (area values) of each component of the standard sample. The liquid chromatograph apparatus according to claim 5, wherein
The information displayed on the operation history confirmation screen when the coincidence of the calibration curves is lower than the threshold includes analysis conditions, vials, concentrations, baseline measurement results, chromatographic top peak values, chromatographic valley values, chromatograms. A liquid chromatograph apparatus comprising at least one of an area value of a graph, a chromatograph, and data at the time of creating a calibration curve. The liquid chromatograph apparatus according to claim 1,
The liquid chromatograph apparatus characterized in that the accuracy of the measured value on the threshold setting screen is one of a peak identification value, a statistical value, and a system suitability test (SST) value. A pump that sucks the eluent, an autosampler that injects the sample into the eluent, a column oven that separates the sample into components, a detector that detects each separated component, and a detector obtained through the detector. In a liquid chromatograph apparatus comprising a data processing device for calculating the concentration of each component from the chromatograph, and an input device and a display device connected to the data processing device,
The display device displays a threshold setting screen or an operation history confirmation screen according to a command from the input device,
The threshold value setting screen includes an input field for inputting a threshold value of a matching degree of a calibration curve used for analysis of a chromatograph represented by the following formula, and the operation history when the matching degree of the calibration curve is smaller than the threshold value. It has a specification field for specifying information to be displayed on the confirmation screen,
The operation history confirmation screen includes an operation history in the data processing apparatus executed until obtaining the calibration curve, a threshold value of the coincidence of the calibration curve input in the input field of the threshold setting screen, and the threshold value A liquid chromatograph characterized by displaying information designated in a designation field of a setting screen.

Here, xi is the concentration of each component of the standard sample, and yi is the measured value (area value) of each component of the standard sample. fxi is the value of the Y coordinate of the point on the calibration curve corresponding to the concentration of each component of the standard sample. yavg is an average of measured values (area values) of each component of the standard sample. The liquid chromatograph apparatus according to claim 8,
The information displayed on the operation history confirmation screen when the coincidence of the calibration curves is lower than the threshold includes analysis conditions, vials, concentrations, baseline measurement results, chromatographic top peak values, chromatographic valley values, chromatograms. A liquid chromatograph apparatus comprising at least one of an area value of a graph, a chromatograph, and data at the time of creating a calibration curve. The liquid chromatograph apparatus according to claim 8,
The operation history displayed on the operation history confirmation screen includes an operation content executed by the data processing device and an operation screen displayed on the display device when the operation content is executed. A liquid chromatograph apparatus. The liquid chromatograph apparatus according to claim 8,
The operation history confirmation screen includes an operation history display area for displaying the operation history. The operation history display area includes an area for displaying an operation procedure number, an area for displaying an operation procedure screen, and an operation procedure operation. A liquid chromatograph apparatus including an area for displaying contents, a comment for an operation procedure, or a button for editing an area for display and an operation procedure. The liquid chromatograph apparatus according to claim 8,
The display device displays an operation history storage screen according to a command from the input device, and the operation history storage screen has a field for inputting a file name, and the file name is input via the input device. And a liquid chromatograph apparatus in which the operation history displayed on the operation history confirmation screen is stored as a file. A pump that sucks the eluent, an autosampler that injects the sample into the eluent, a column oven that separates the sample into components, a detector that detects each separated component, and a detector obtained through the detector. In a liquid chromatograph analysis method using a liquid chromatograph device including a data processing device that calculates the concentration of each component from the chromatograph, and an input device and a display device connected to the data processing device,
In accordance with a command from the input device, an input field for inputting a threshold value for the coincidence of calibration curves used for chromatographic analysis on the display device, and information to be displayed when the coincidence of the calibration curves is smaller than the threshold value Displaying a threshold setting screen having a designation field to designate;
Setting analysis conditions based on input data input via the input device;
Setting sample conditions based on input data input via the input device;
Setting a calibration curve threshold value based on input data input via the input device; and
Performing a liquid chromatograph analysis on a standard sample having a known component concentration using the data processing device, and creating a calibration curve;
Using the data processing apparatus, calculating the coincidence of the calibration curves according to the following equation:

[Where xi is the concentration of each component of the standard sample, and yi is the measured value (area value) of each component of the standard sample. fxi is the value of the Y coordinate of the point on the calibration curve corresponding to the concentration of each component of the standard sample. yavg is an average of measured values (area values) of each component of the standard sample. ]
Using the data processing device, determining whether the calibration curve coincidence is less than a threshold of the calibration curve coincidence;
When the matching degree of the calibration curve is smaller than a threshold value of the matching degree of the calibration curve, the operation history until the liquid chromatographic analysis result is obtained and the information designated in the designation column are displayed on the display device. And steps to
A liquid chromatographic analysis method comprising: The liquid chromatograph analysis method according to claim 13,
In accordance with a command from the input device, the operation history in the data processing device executed until the analysis result of the chromatograph is obtained on the display device, and the calibration curve input in the input column of the threshold setting screen Displaying an operation history confirmation screen that displays the threshold value of the matching degree and information specified in the specification field of the threshold setting screen;
A liquid chromatographic analysis method comprising:

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