JP5046665B2 - Novel microorganism and drainage purification method using the same - Google Patents

Description

  The present invention relates to a novel microorganism having an excellent ability to decompose organic matter and a method for purifying wastewater using the same.

  Conventionally, methods for purifying waste water using microorganisms or decomposing sludge have been widely used.

  For example, in Patent Document 1, in order to effectively decompose difficult-to-decompose organic substances such as dyes present in wastewater, carbon fiber materials into which oxygen groups have been introduced include Bacillus carbonifils, Bacillus films, A method for purifying wastewater by immobilizing a plurality of types of bacteria selected from Bacillus pumilus, Pseudomonas, and the like and bringing the immobilized microorganisms into contact with wastewater containing persistent organic substances is described.

JP 2002-86178 A

In the waste water purification method described in Patent Document 1, a plurality of types of bacteria are used, but in this case, it is unclear which bacteria are performing an effective activity, and therefore management and control of the organic matter decomposition ability is performed. In some cases, the purification capacity may become unstable.
Therefore, an object of the present invention is to provide a novel microorganism excellent in organic matter decomposing ability, and a wastewater purification method that can easily manage and control the organic matter decomposing ability.

In view of the above problems, the present inventor has conducted various intensive studies, and as a result, has isolated a novel gram-positive gram-positive belonging to the genus Bacillus pumilus, which has been isolated and purified from kitchen effluent and food factory effluent. Spore gonococci (a new microorganism having a deposit number of FERM P-21162 , deposited on the Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology, and received on January 22, 2007) has excellent organic substance decomposing ability. As a result, the present invention has been conceived.

That is, the waste water purification method of the present invention is characterized in that Bacillus pumilus having the accession number FERM P-21162 having the ability to decompose organic matter is mixed with waste water to decompose the organic matter in the waste water.

Bacillus pumilus with the accession number FERM P-21162 has excellent ability to decompose oils, fats, proteins, starches, etc., and can exhibit excellent drainage purification ability by itself. It is easy to manage and control and can be a wastewater purification method with excellent organic matter decomposition ability. In this microorganism, no virulence factor (hemolytic toxin, DNA-degrading enzyme, vomiting toxin gene) is recognized, and since it is not pathogenic, it is harmless to humans. Therefore, by putting the culture solution containing the microorganism of the accession number FERM P-21162 into wastewater containing organic matter such as kitchen wastewater and food factory wastewater, the wastewater can be purified without burdening the environment.

In addition, it is desirable to aerate the waste water into which the new microorganism belonging to the genus Bacillus pumilus having the accession number FERM P-21162 is added. By aeration, oxygen can continue to be fed into the waste water, and the organic matter decomposition of the Bacillus pumilus can be promoted.

Here, Table 1 shows the mycological properties of a novel microorganism belonging to the genus Bacillus pumilus, under the accession number FERM P-21162 .

According to the present invention, by using a novel microorganism belonging to the genus Bacillus pumilus, the accession number FERM P-21162 obtained by isolation and purification culture from kitchen wastewater or food factory wastewater, It can be set as the waste water purification method and fertilizer manufacturing method which are easy to manage and control. In addition, since the microorganism of the present invention is not pathogenic, it can be a wastewater purification method that does not place a burden on the environment.

The method for purifying wastewater of the present invention comprises a culture solution of a novel microorganism belonging to the genus Bacillus pumilus (hereinafter referred to as “the present bacterium”) having a deposit number of FERM P-21162 , and kitchen wastewater and food stored in a treatment tank. It is thrown into factory wastewater and decomposes and purifies organic substances contained in wastewater. The treatment tank is constantly aerated, thereby further promoting organic matter decomposition of the bacterium. Here, the processing tank used in the present embodiment may have any size and shape.

  In addition, it is desirable to add the culture solution of the present bacterium in an amount of 1/2000 to 1/100 of the capacity of the waste water. When the input amount is less than 1/2000, the purification effect is drastically reduced. Further, the relationship between the input amount and the decomposition power of the organic matter is saturated when the input amount is about 1/100 of the capacity of the waste water. Therefore, even if it inputs more than this, an effect does not change so much, Unnecessarily many fungus liquids will be used, and cost will become high.

The waste water purification method in the present embodiment is as follows.
First, an aeration apparatus (aeration pipe and air pump) is attached to the drainage facility. Next, the contents such as oil and sludge adhering to the grease strap are cleaned. In the case of a purification facility such as a detoxification facility, it can be left as it is. Thereafter, a culture solution of the bacterium of about 1/2000 to 1/100 of the drainage capacity is injected once daily. For example, in a 400L grease strap, it is 200mL-4L. The amount of the culture solution is adjusted according to the effluent pollution degree. The culture solution is preferably injected at night when drainage is stopped in the grease trap of the restaurant. In this case, since this bacterium is proliferated nearly 1000 times in the tens of hours until the next morning, a high purification effect is expected. In addition, by using an automatic injection device that combines a pump and a timer in the culture solution tank, a fixed amount of culture solution can be injected every day at a fixed time.

Hereinafter, the isolation method and purification ability of Bacillus pumilus of the accession number FERM P-21162 according to the embodiment of the present invention will be described in detail.

1-1: Isolation method Sewage samples were collected from many grease traps, and the diluted solution was inoculated into a fat and oil degradation test medium (medium consisting of Tween 20 and inorganic salts) and then cultured at 30 ° C. for 3 days. Then, using a platinum wire from the medium in which a colony of oleolytic bacteria was obtained with a high number of bacteria, using a platinum wire, suspending this in sterile physiological saline, inoculating into a gravy agar medium, forming a colony, About 100 pure bacterial strains were obtained. These bacterial isolates were tested for fat, starch, and protein resolution, and several strains with strong resolution in all three were obtained. Further, a test on pathogenic factors (hemolytic toxin, DNA degrading enzyme, and emetic toxin gene) was performed, and a strain having no pathogenic factor was selected to obtain the bacterium.

1-2: Measurement of oil-and-fat resolving power of this bacterium The present bacterium isolated by the method described in 1-1 was inoculated into kitchen drainage in an amount of 1/100 and aerated at 30 ° C. for 24-48 hours. FIG. 1 shows the change over time of the n-hexane extract (oil concentration) contained in the kitchen waste water at this time. In addition, as a comparative example, the time-dependent change of the n-hexane extract (fat concentration) of the kitchen wastewater which does not inoculate this microbe is also shown in FIG.

  As shown in FIG. 1, with the passage of time, the n-hexane extract (oil concentration) decreases due to the effect of the resident bacteria in the kitchen drainage even when the bacteria are not inoculated (white circles in FIG. 1). It was found that the purification of kitchen wastewater was promoted by inoculation of (indicated by black circles in FIG. 1).

1-3: Measurement of kitchen drainage purification ability of the present bacteria 1/100 amount of the present bacteria isolated by the method described in 1-1 was inoculated into the kitchen drainage and aerated at 30 ° C. for 24 to 48 hours. FIG. 2 shows the change over time in the biochemical oxygen demand (BOD), which is one of the indexes representing the degree of contamination of kitchen wastewater at this time. In addition, as a comparative example, the time-dependent change of the n-hexane extract (oil concentration) of the kitchen drainage which does not inoculate this microbe is also shown in FIG.

  As shown in FIG. 2, with the passage of time, BOD decreases due to the effect of resident bacteria in the kitchen drainage even when the bacteria are not inoculated (white circle in FIG. 2). Was found to be promoted (black circle in FIG. 2).

1-4: Relationship between the ability to purify the kitchen wastewater and the growth ability of the fungus Inoculate 1/2000 amount of the fungus isolated by the method described in 1-1 into a sterilized kitchen wastewater at 30 ° C for 24 hours. Aerated for ~ 48 hours (Measurement A). In addition, non-sterile kitchen wastewater was inoculated with the same amount of the bacterium as in measurement A and aerated at 30 ° C. for 24-48 hours (measurement B). FIG. 3 shows the change over time of the n-hexane extract (oil concentration) contained in the kitchen waste water at this time.

As shown in FIG. 3, in measurement A, the fat concentration of about 1000 mg / L decreased to about 75% in 24 hours and to about 30% in 48 hours. At this time, as indicated by C in FIG. 3, the number of bacteria of the bacterium increased about 10 times from 10 ⁶ CFU / mL to 10 ⁹ CFU / mL in 24 hours. It was found that it grew using oil and fat as nutrients.

  On the other hand, as shown in FIG. 3, in the measurement B, the fat and oil content decreased to about 35% in 24 hours and decreased to about 15% in 48 hours. From this result, it was found that the present bacterium was used for wastewater purification in cooperation with the resident bacteria in the kitchen effluent. The difference between measurement A and measurement B is the amount of fats and oils decomposed by resident bacteria.

  In addition, since this bacterium exhibits an excellent ability to decompose organic matter even by itself, it is estimated that it can be composted with livestock manure without burdening the environment by mixing it with livestock manure. Is done.

According to the present invention, a novel microorganism belonging to the genus Bacillus pumilus having an accession number of FERM P-21162 is excellent in the ability to decompose oils, fats, proteins, starches, etc., and can exhibit an excellent ability to decompose organic substances alone. Therefore, it is useful as a wastewater purification method that has a high decomposing ability and is easy to manage and control the organic substance decomposing ability.

It is a measurement result of the time-dependent change of n-hexane extract (oil and fat concentration) contained in kitchen wastewater. The graph shown with white circles in the figure is not inoculated with this bacteria, and the graph with black circles is inoculated with this bacteria. It is a thing. It is a measurement result of the time-dependent change of BOD of kitchen drainage, and the graph shown by a white circle in the figure is not inoculated with the present bacteria, and the graph shown with a black circle is inoculated with the present bacteria. It is a measurement result of a time-dependent change of the n-hexane extract (oil-fat density | concentration) contained in kitchen wastewater.

Claims (3)

A novel microorganism belonging to the genus Bacillus pumilus having an accession number of FERM P-21162 , which has the ability to decompose organic substances contained in wastewater such as kitchen wastewater and food factory wastewater . New microorganisms belonging to the genus Bacillus pumilus under the accession number FERM P-21162 that have the ability to decompose organic matter contained in wastewater such as kitchen wastewater and food factory wastewater, and wastewater containing organic matter such as kitchen wastewater and food factory wastewater. And a method for purifying waste water, which comprises mixing organic matter into the waste water and decomposing organic matter in the waste water.   The waste water purification method according to claim 2, wherein the waste water mixed with the new microorganism belonging to the genus Bacillus pumilus is aerated.

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