JP4999588B2 - Container device, DNA testing device including the same, and method for penetrating the same - Google Patents

Description

  The present invention relates to a container apparatus having a container filled with a solution, a DNA testing apparatus including the container apparatus, and a method for penetrating the same.

  Various types of reagents are used in the DNA (Deoxyribonucleic Acid) inspection process. In order to handle such a reagent easily with an automatic apparatus, a container apparatus having a so-called prepacked reagent container that is pre-filled with an amount to be used in one inspection is used.

  The pre-packed reagent container has a configuration in which a plastic container is filled with a reagent and a member for sealing the reagent is fixed to the opening. Further, the reagent container is configured to prevent the reagent from leaking out by a sealing member that seals the reagent, and to withstand storage for a long period from manufacture to start of use.

  When the container is used for testing, the container is set in a DNA testing apparatus, and a convex drilling member with a sharp tip disposed in the apparatus descends toward the container. The sealing member is broken by the drilling member. As described above, it is possible to take out the reagents in the container or add a liquid to the container (see Patent Document 1).

In the configuration in which the DNA testing apparatus has a drilling member, the following problems may occur.
(1) The reagent adhering to the back surface of the sealing member moves to a drilling cutter that is a drilling member when a hole is drilled.
(2) The foreign matter and dust adhering to the surface of the sealing member move to the drilling cutter when the hole is drilled.

  When the punching cutter is left wet with the reagent, surrounding foreign matter and dust are likely to adhere. The foreign matter and dust adhering to the punching cutter will adhere to the surface of the sealing member when a hole is made in the container after the next inspection with the punching cutter. When the pipette tip is allowed to enter the container, dirt including foreign matter and dust attached to the surface of the sealing member adheres to the outer peripheral surface of the pipette tip. When the pipette tip enters deeply into the solution, dirt attached to the outer peripheral surface flows out into the solution. The contamination inhibits the reaction of the reagent in the container.

  In particular, in a reaction using a reagent (enzyme) that is easily affected by foreign substances such as PCR (Polymerase Chain Reaction), there is a serious effect that nucleic acid is not amplified.

  As a means for solving the above-mentioned problem, a configuration in which a film as a sealing member is broken with a lid covering the opening of the container is conceivable. Patent Documents 2 and 3 disclose container devices having this fracture configuration.

  In the container device of Patent Document 2, the seal breaker 9 that functions as a sealing member that seals the opening of the container also functions as a penetrating means that breaks and penetrates the sealing member (see FIG. 14). A fracture protrusion 18 that breaks the seal member is formed integrally with the seal breaker 9 on the back side (solution side) of the seal breaker 9. When a container is used for DNA testing, the breaker protrusion 18 enters the container by pushing the seal breaker 9 by hand and pushing down the breaker protrusion 18 toward the container.

In the container device of Patent Document 3, a piston member 24 having a sharp tip is disposed above the seal member 26 (see FIG. 15). The piston member 24 functions as a penetrating means for penetrating the seal member 26 and a sealing member for preventing the solution from leaking out of the container. A hex protrusion 40 is formed integrally with the piston member 24 on the top of the piston member 24. The piston member 24 is pushed down toward the well from which the reagent flows by the pushing member from the outside that contacts the hex projection 40. By pushing down the piston member 24 in this way, the seal member 26 is broken by the piston member 24.
JP 2003-329696 A JP 2006-29817 A Japanese Patent No. 3823053

  In both container devices, a configuration is disclosed in which a rupture protrusion that functions as a penetrating means for penetrating a seal member and a tip portion of a piston member are integrated with a lid portion that seals the solution in the container. However, the container devices disclosed in Patent Documents 2 and 3 have the following problems.

  The container device disclosed in Patent Document 2 has a configuration in which the fracture protrusion of the seal breaker directly touches the seal member. For this reason, when the sealing member is penetrated by the breaking protrusion, the foreign matter and dust attached to the surface of the breaking protrusion move to the surface of the sealing member. Thereafter, when the pipette tip is inserted into the container, the seal member to which foreign matter and dust adhere is brought into contact with the pipette tip. At that time, foreign matter and dust adhering to the surface of the seal member move to the outer peripheral surface of the pipette tip. When this pipette tip enters deeply into the container and comes into contact with the solution contained in the container, foreign matter and dust on the outer peripheral surface of the pipette tip may be mixed into the solution. Due to this contamination, there is a problem that the nucleic acid is not amplified in the nucleic acid amplification step as described above.

  Further, in the state where the seal breaker covers the opening of the container, the length of the breaking protrusion in the direction in which the breaking protrusion enters the container is added to the length in the height direction of the container. . That is, the height of the container device is increased as compared with a container device having no breaking protrusion. Accordingly, there is a problem that the container device including the container is increased in size by the size of the breaking protrusion.

  On the other hand, in the container device disclosed in Patent Document 3, the piston member is in direct contact with the seal member. Therefore, when the seal member is penetrated by the piston member, foreign matter and dust attached to the surface of the piston member move to the surface of the seal member. Thereafter, when the pipette tip is inserted into the container, the seal member to which foreign matter and dust adhere is brought into contact with the pipette tip. At that time, foreign matters and dust on the seal member move to the outer peripheral surface of the pipette tip. When this pipette tip enters deeply into the container and comes into contact with the solution contained in the container, there is a risk that foreign matter and dust on the outer peripheral surface of the pipette tip will be mixed into the reagent. Due to this contamination, there is a problem that the nucleic acid is not amplified in the nucleic acid amplification step as described above.

  Further, the hex protrusion is formed integrally with the piston member and functions as a pushing means for pushing the piston member into the container. The piston member integrally having the hex protrusion is inevitably higher than the case where there is no hex protrusion by the height of the side wall of the hex protrusion. Accordingly, there is a problem that the entire container device having the piston member is larger than the container device having the piston member having no hex protrusion.

  Containers used in DNA testing devices are often disposable, and it is assumed that many containers are purchased and stored together. Since the storage space is desired to be as small as possible, an increase in the size of the container is not preferable because it leads to an increase in the storage space. In particular, the increase in the height of the container device including the container and the seal member as described above is more preferable because the height of the storage space is limited when another container device is placed on the container device. Absent.

  The present invention has been made in view of the above circumstances, and a container device that can prevent surrounding foreign matter and dust from entering the container while preventing an increase in the size of the container, a DNA test apparatus including the same, and a DNA testing apparatus therefor An object is to provide a penetration method.

The container device of one embodiment of the present invention includes a plurality of openings that are arranged, a plate that includes a plurality of containers that can store liquid through the openings, and a liquid that covers each of the plurality of openings individually. a sealing member which is the first sealing is means for, and a holding member for holding the thin isolating member and isolation member for covering the sealing member, each of the plurality of openings A second sealing means, and a penetrating means that can move from above the separating member and break the sealing member via the separating member. The separating member is sealed by the penetrating means. The member is not broken when the member is broken, the penetrating means can be prevented from coming into contact with the sealing member , and the second sealing means can open and close the opening via the holding member. .

In the method for penetrating a container device of one embodiment of the present invention, a container in which a liquid is contained, an opening is covered with a sealing member, and the sealing member is covered with a thin isolation member held by a holding member A step in which the penetrating means moves from above the separating member and breaks the sealing member through the separating member, a step in which the separating member is moved through the holding member to open the opening, The isolation member is not broken when the penetrating means breaks the sealing member, and can prevent the penetrating means from coming into contact with the sealing member.

  According to the present invention, the separating member is not broken when the penetrating means breaks the sealing member, and the penetrating means can be prevented from coming into contact with the sealing member. Thereby, the penetration means and the liquid in the container do not come into contact with each other. Furthermore, the isolation member is thin. Therefore, the height of the container device having the height of the container and the thin thickness of the separating member can be suppressed. Therefore, it is possible to provide a container device that can prevent surrounding foreign matter and dust from entering the container while preventing the container from becoming large, a DNA testing apparatus including the container device, and a method for penetrating the same.

The best mode for carrying out the present invention will be described with reference to the drawings. The container apparatus constituting the amplification unit of the DNA testing apparatus to which the present invention is applied will be described in detail.
(First embodiment)
FIG. 1 shows a schematic diagram of a DNA test apparatus according to an embodiment of the present invention.

  The DNA testing apparatus 1 includes an extraction unit 2, an amplification unit 3, a hybridization unit 4, and a detection unit 5. The extraction unit 2 extracts DNA from the biological sample. In the amplification unit 3, the DNA extracted in the extraction unit 2 is amplified. The amplification unit 3 includes the container devices 100 and 118 according to the first embodiment or the second embodiment of the present invention. In the hybridization unit 4, the DNA amplified by the amplification unit 3 is combined with the probe DNA. The detection unit 5 detects whether or not the DNA bound by the hybridization unit 4 is reliably bound to the probe DNA. Further, the DNA testing apparatus 1 includes a transport unit, a pipette unit 6, a pipette transport unit, a pipette tip holding unit, a pipette disposal unit, and the like in addition to the above-described components (the DNA after the transport unit and the pipette transport unit). (Not shown with the inspection device component). The transport unit transports the PCR plate 8 shown in FIG. The pipette unit 6 supplies and discharges the solution to the PCR plate 8 and other parts. The pipette disposal unit accommodates used pipette tips.

  The pipette unit 6 is configured to be movable up and down, front, back, left and right as indicated by arrows 7 by a pipette transport unit using a lead screw (not shown), and moves the solution between extraction, amplification and hybridization steps.

  FIG. 2 shows the PCR plate 8. 8 × 12 sample tubes are configured. The bottom portion 9 (tip portion) of each container has a shape that can be fitted to a metal block described later. In this embodiment, the PCR plate is pre-filled with a solution for purifying the solution after PCR in addition to the enzyme used for PCR.

  In the opening 10 of the container, a laminate film made of aluminum and resin is fixed by adhesion, heat welding or ultrasonic welding in order to prevent foreign matters from entering from the outside. Thus, the solution is prevented from evaporating and can be stored for a long period of time. In FIG. 2, the state which abbreviate | omitted the laminated film, the sealing member mentioned later, and its surrounding mechanism member is shown.

  FIG. 3 shows a cross-sectional view of the container device including the PCR plate (the side view of FIG. 2 for the PCR plate). The container device 100 includes a PCR plate 8, a laminate film 102, an elastic member 103, a punching cutter 108, and an actuator 109.

  The PCR plate 8 is provided with a container 110 that can store a solution 101 as a reagent liquid through the opening 10. The solution 101 is filled in the container 110 in advance before the amplification reaction. A plate portion 119 extends laterally from the side surface of the side wall constituting the container 110. A frame 104 is provided on the upper end surface of the plate portion 119, and the frame 104 extends from the upper end surface of the plate portion 119 upward with an inverted L-shape. The frame 104 is a holding member that holds the elastic member 103. The frame 104 includes a hinge part 105 and a convex part 107. The lower end of the hinge portion 105 is fixed to the upper end surface of the PCR plate 8, and the hinge portion 105 can rotate with the corner portion 106 as a fulcrum F. The frame 104 including the hinge portion 105 is removed from the opening 10 of the container 110 by being rotated around the fulcrum F by the actuator 109. The convex portion 107 is fitted to the container 110, and the inner peripheral surface thereof is in close contact with the outer peripheral surface of the side wall of the container 110.

  The laminate film 102 has a first surface 1021 that faces the container 110 and a second surface 1022 that forms a surface opposite to the first surface 1021. The first surface 1021 and the second surface 1022 are substantially parallel, and the distance between the first surface 1021 and the second surface 1022 is small. Thereby, the laminate film 102 has a thin film shape. The laminate film 102 is fixed to the upper end surface of the side wall of the container 110 and covers the opening 10 of the container 110. Thereby, the laminate film 102 functions as a sealing member that prevents the solution 101 in the container 110 from leaking out of the container 110 and prevents foreign matter and dust from entering the solution 101 from the outside.

  The elastic member 103 is formed to have the same size as the diameter of the outer peripheral surface of the side wall that forms the opening 10 of the container 110, and has a thin film shape. The elastic member 103 is disposed between the laminate film 102 and the punching cutter 108. The elastic member 103 is an isolation member that functions as an isolation coating means for isolating the laminate film 102 and the punching cutter 108 and covering the laminate film 102. The elastic member 103 is not broken when the punching cutter 108 breaks the laminate film 102, and can prevent the punching cutter 108 from coming into contact with the laminate film 102. Furthermore, since the elastic member 103 is an elastic body, the elastic member 103 is deformably deformed. The side surface of the elastic member 103 and the convex portion 107 of the frame 104 are in close contact with each other, and one end of the elastic member 103 is held by the frame 104. During the amplification reaction, the elastic member 103 covers the opening 10 of the container 110 with the laminate film 102 interposed therebetween. After completion of the amplification reaction, the opening 109 covered with the elastic member 103 is opened by rotating the elastic member 103 about the fulcrum F in FIG. 3 clockwise by the actuator 109.

  The material and the thickness of the thin-film elastic member 103 are determined so as to satisfy the conditions that are not cut or avoided when the punching cutter 108 comes into contact. The material is selected from synthetic rubber and natural rubber. Moreover, the plastic material shape | molded in the shape which elastically deforms may be sufficient.

  The perforating cutter 108 functions as a penetrating means that can break the laminate film 102 via the elastic member 103. The punching cutter 108 has a convex tip that can punch a hole in the laminate film 102. Further, the punching cutter 108 is made of a metal material such as stainless steel or aluminum and a plastic material. The hole punching cutter 108 is moved vertically and horizontally in FIG. 3 by a drive source (not shown) configured on the DNA testing apparatus 1 side. The hole cutter 108 has a slightly rounded tip so as not to break the thin elastic member 103 when making a hole in the laminate film 102.

  The actuator 109 functions as a rotating means for rotating the elastic member 103 and is moved by a drive source (not shown). The actuator 109 acts on the convex portion 107 formed on the frame 104 to open and close the frame 104. The actuator 109 rotates the elastic member 103 with the corner portion 106 at one end of the frame 104 as a holding member serving as a fulcrum F.

  Next, the configuration of the container device 100 of the amplification unit 3 during the amplification reaction will be described with reference to FIG.

  The metal block 11 is fitted with the PCR plate 8. The metal block 11 is made of a metal having good thermal conductivity such as aluminum or copper alloy, and 96 wells (holes) are formed as dotted lines.

  A Peltier element 12 for heating the metal block 11 is disposed below the metal block 11. It is necessary to securely transfer heat from the Peltier element 12 to the metal block 11 by bringing the contact surface of the metal block 11 in contact with the Peltier element 12 into close contact with the surface of the Peltier element 12. Therefore, grease (not shown) is applied to the contact surface of the metal block 11 and the contact surface of the Peltier element 12. A sheet material having good thermal conductivity may be used as a material other than grease to obtain a similar thermal conductivity effect.

  Below the Peltier element 12 is a base plate 13 on which the components of the amplifying unit 3 are arranged. The base plate 13 has a hollow structure provided with openings 14 and 15, and has a structure in which cooling water flows through piping (not shown) connected to the openings 14 and 15. When the temperature of the metal block 11 is lowered, cooling of the lower surface of the Peltier element 12 is promoted by this cooling water.

  A heating plate 16 made of a metal having good heat conductivity such as aluminum or copper alloy is disposed on the PCR plate 8. A Peltier element 17 is disposed on the heating plate 16. It is necessary to securely transfer heat from the Peltier element 12 to the heating plate 16 by bringing the contact surface of the heating plate 16 in contact with the Peltier element 12 into close contact with the surface of the Peltier element 12. Therefore, grease (not shown) is applied to the contact surface of the heating plate 16 and the contact surface of the Peltier element 12.

  A cooling block 18 made of a metal material is fixed to the contact surface between both the Peltier elements 17 and the cooling block 18 with grease in the same manner as described above. When heat is radiated from the upper surface of the Peltier element 17, heat radiation is promoted by cooling by the cooling block 18.

  The cooling block 18 has a hollow structure provided with openings 19 and 20, and has a structure in which cooling water flows through piping (not shown) connected to the openings 19 and 20.

  The heating plate 16, the Peltier element 17 and the cooling block 18 are configured as one unit (top plate unit 21). The top plate unit 21 is configured to be movable by driving means (not shown).

  When the user sets the PCR plate 8 in the amplification unit 3, the top unit 21 is in the retracted position and does not hinder the setting operation. During the amplification reaction, the PCR plate 8 is heated from above at the heating position.

  Next, the description of the present invention will proceed based on the operation of the DNA testing apparatus.

  First, a container filled with a reagent is set in each of the extraction, amplification, and hybrid process units of the DNA testing apparatus 1. In the extraction unit 2, biological samples such as blood and urine are set, and a solution containing DNA is obtained by a known means. The PCR plate 8 is set in the amplification unit 3 until the extraction is completed.

  The nucleic acid solution containing DNA is sucked into a pipette tip (not shown) attached to the pipette section 6 and moved to the amplification section 3. At this time, the pipette tip is replaced with a new one from the one used in the extraction unit 2.

  Prior to the movement of the solution, it is necessary to make a hole in the laminate film 102 covering the amplification container 110 at the movement destination.

  FIG. 5 shows the state of the container device immediately before drilling. The nucleic acid solution 101 is accommodated in the container 110 through the opening 10 of the container 110. The solution 101 is sealed by covering the opening 10 of the container 110 with the laminate film 102. A thin elastic member 103 is disposed above the laminate film 102 and the laminate film 102 is covered with the elastic member 103. Thus, the container 110 covered with the laminate film 102 and the elastic member 103 is provided. The punching cutter 108 is disposed and positioned above the elastic member 103 of the container 110 by a driving source (not shown). From this state, the punching cutter 108 is moved downward by a drive system (not shown) to bring the punching cutter 108 into contact with the elastic member 103. When the punching cutter 108 is further lowered in the direction of the solution 101, the elastic member 103 is elastically deformed toward the solution 101 side. Thereafter, the elastic member 103 contacts the second surface 1022 of the laminate film 102. When the hole punching cutter 108 further enters the container 110, the hole punching cutter 108 breaks the laminate film 102 via the elastic member 103. When the punching cutter 108 breaks the laminate film 102, the elastic member 103 can prevent the punching cutter 108 and the laminate film 102 from contacting each other without being broken. When the punching cutter 108 is lowered to a predetermined position, a hole through which the pipette tip can pass is formed in the laminate film 102. FIG. 6 shows a state in which the punching cutter 108 has reached the lowest point after the laminate film 102 has been torn.

  When the punching cutter 108 is raised from this state, the elastic member 103 returns to its original shape by the restoring force, and becomes as shown in FIG. At this time, the shape of the elastic member 103 does not sufficiently return to be slightly bent toward the solution 101 side.

  The hole cutter 108 is returned to the standby position after returning to the same position as in FIG.

  Next, as shown in FIG. 8, the actuator 109 on the main body side of the DNA testing apparatus 1 is hooked on the convex portion 107 of the frame 104, and the hinge portion 105 is rotated around the corner portion 106 that functions as the fulcrum F. As a result, the container 110 is released from the covering of the elastic member 103 at the opening 10 and is opened. In FIG. 8, the actuator 109 is at the upper right and the frame 104 and the elastic member 103 are almost upright. The actuator 109 is driven so as to follow the rotation of the hinge portion 105 as shown in FIG.

  After the pipette tip that has sucked the nucleic acid solution is moved directly above the container 110, it is lowered to the discharge position. Then, the solution in the pipette tip is discharged into the container 110. At this time, the pipette tip may be driven while the actuator 109 is kept open. Alternatively, the actuator 109 may be separated from the convex portion 107 after being opened, and the open state of the frame 104 may be maintained by a guide member (not shown) disposed near the pipette tip during pipetting.

  As described above, the punching cutter 108 acts on the laminate film 102 via the elastic member 103, deforms the laminate film 102, and then breaks it. In this broken state, the elastic member 103 prevents the punching cutter 108 from coming into contact with the laminate film 102 without being broken. Thereby, the solution 101 in the container 110 does not contact the punching cutter 108. Therefore, the solution 101 does not adhere to the surface of the punching cutter 108. Even if foreign matter or dust is attached to the surface of the punching cutter 108, the surface of the elastic member 103 that is interposed between the punching cutter 108 and the solution 101 receives foreign matter or dust that has fallen from the surface of the punching cutter 108. Therefore, foreign matter and dust can be reduced from falling on the second surface 1022 of the laminate film 102, and the possibility of foreign matter and dust being mixed into the solution 101 can be avoided.

  The elastic member 103 functions as a means for covering the opening 10 of the container 110 and sealing the solution 101 after the laminate film 102 is perforated. Since the elastic member 103 has a thin film shape, the height of the container device 100 having the height of the container 110 and the thin thickness of the elastic member 103 can be suppressed as compared with the conventional case. Therefore, the container device can be made smaller than before.

  Next, the amplification process will be described. When the solution 101 is discharged into the predetermined container 110, the pipette tip is withdrawn from the PCR plate 8. The frame 104 that has been in the open position is moved in the direction of the container 110 by the actuator 109 as shown in FIG. The retracted top panel unit 21 returns to a position facing the frame 104 and the elastic member 103. Next, the PCR plate 8 and the metal block 11 are raised from below in the figure by a drive mechanism (not shown) and pressed against the heating plate 16 of the top plate unit 21. As a result, the bottom 9 of the PCR plate 8 and the fitting portion of the metal block 11 are brought into close contact with each other, resulting in the state shown in FIG. An enlarged side view of the PCR plate 8 at this time is as shown in FIG. The heating plate 16 contacts the elastic member 103 held inside the frame 104 and heats the container 110 from above. FIG. 10 is also a right side view of FIG. 4, and PCR is performed in the container 110 in the depth direction of the drawing. At this time, the top plate unit 21 has a structure capable of pressing the container of the PCR plate 8 with a force of 50 to 100 gf per place, or more in some cases.

  PCR is started in such a state that the container 110 is pressed. The DNA in the container 110 is amplified by performing PCR at a predetermined holding time and cycle number between about 92 ° C. and 55 ° C. and 72 ° C.

  After completion of DNA amplification, the top unit 21 is retracted by the same procedure as described above. Furthermore, the frame 104 and the elastic member 103 are opened by the actuator 109, and the amplification product in each container is sucked with a pipette tip and conveyed to a purification unit (not shown). The frame 104 and the elastic member 103 are closed again by the actuator 109.

Containers similar to extraction containers and PCR plates are also set in the purification section, and the containers are prefilled with magnetic particles, purification liquid, washing liquid, elution liquid and the like used for purification. The movement of the pipette tip when the amplification product is discharged into the purification container is the same as when the solution is moved from the extraction unit 2 to the amplification unit 3, and the description thereof is omitted.
(Second embodiment)
In the container device according to the first embodiment of the present invention, the punching cutter as the punching means is constituted by a single member. However, the present invention is not limited to this configuration. That is, the drilling means can be composed of a plurality of members. In the container device according to the second embodiment, a punching means composed of two members (a pressing member and a cutting member) is defined.

  A container apparatus according to a second embodiment of the present invention will be described in detail with reference to FIGS. The same components as those in the first embodiment are denoted by the same reference numerals.

  The penetrating means for breaking the laminate film 102 of the container device 118 of the second embodiment includes a punching cutter 111 and a pressing member 112 which are cutting members. The punching cutter 111 is detachable from the pressing member 112.

  The hole punching cutter 111 is fixed to the PCR plate 8 by holding means (not shown). The tip of the punching cutter 111 is sharp and is configured to be able to break the laminate film 102 via the elastic member 103.

  The pressing member 112 presses the punching cutter 111 to cause the punching cutter 111 to enter the container 114 via the elastic member 103. The outer peripheral surface of the pressing member 112 is formed so as to tightly contact the inner peripheral surface forming the concave portion of the punching cutter 111. The frame 104 is formed integrally with the hinge portion 105. Convex portions 107 are provided at the ends of the frame 104 as in the first embodiment. The frame 104 has an inner peripheral surface that can be press-fitted into the container 114, and rotates around a corner portion 106 that functions as a fulcrum F. As in the first embodiment, the frame 104 opens and closes the opening 10 of the container 114 by hooking the projection 107 with an actuator not shown in FIG.

  Next, operation | movement of the container apparatus at the time of making a hole in the laminate film 102 is demonstrated.

  After the pressing member 112 is moved above the punching cutter 111, it is lowered and inserted into the recess of the punching cutter 111 as shown in FIG. Next, as shown in FIG. 12, the punching cutter 111 is moved above the elastic member 103 and then lowered to break the laminate film 102 via the elastic member 103. Thereafter, the punching cutter 111 is further lowered, and the punching cutter 111 is stopped when the flange portion 117 of the punching cutter 111 hits the frame 104. Thereafter, the pressing member 112 and the punching cutter 111 are lifted back to the standby position. Since the pressing member 112 is tightly press-fitted into the recess of the punching cutter 111, the punching cutter 111 is prevented from being detached from the pressing member 112 during the punching operation.

  FIG. 13 is a diagram illustrating a state in which all the containers 114 have been drilled. The punching cutter 111 and the pressing member 112 are moved down after being moved above the cutter holder 115 close to the container 114. When lowered to a certain height, the punching cutter 111 is detached from the pressing member 112 by the removing means 116 provided on the DNA testing device 1 side. The removing means 116 is configured to be movable up and down relatively with the pressing member 112 and pushes down the flange portion 117 of the punching cutter 111. The detached punching cutter 111 is held by a fixing means (not shown) in the cutter holding portion 115. The form in which the hole punching cutter 111 is stored in a predetermined position in the DNA testing apparatus 1 instead of the cutter holding unit 115 may be used.

  As described above, in this embodiment, the penetrating means includes the pressing member 112 and the punching cutter 111. Thereby, the pressing force to the punching cutter 111 of the pressing member 112 (that is, the contact pressure between the outer peripheral surface of the pressing member 112 and the inner peripheral surface forming the concave portion of the punching cutter 111) can be adjusted. Therefore, the pressing force from the punching cutter 111 through the elastic member 103 to the laminate film 102 can be adjusted. Therefore, the size of the through hole provided in the laminate film 102 can be adjusted by adjusting the pressing force.

  Further, the punching cutter 111 is detachable from the pressing member 112. As a result, the pressing member 112 can be reused any number of times as long as the outer peripheral surface in contact with the punching cutter 111 is not worn. On the other hand, the drilling cutter 111 can be replaced with another component or disposed of alone when the inner peripheral surface contacting the pressing member 112 is worn.

  Further, the elastic member 103 is disposed between the punching cutter 111 and the solution 101 at the time when the through-hole is provided in the laminate film 102. Therefore, it becomes the structure of the container apparatus 118 in which the solution 101 in the container 114 does not contact the punching cutter 111. Therefore, the solution 101 does not move to the surface of the punching cutter 111. Even if foreign matter and dust are attached to the surface of the punching cutter 111, the surface of the elastic member 103 interposed between the punching cutter 111 and the solution 101 receives foreign matter and dust that has fallen from the surface of the punching cutter 111. Therefore, foreign matter and dust can be reduced from falling on the second surface 1022 of the laminate film 102, and the possibility of foreign matter and dust being mixed into the solution 101 can be avoided.

  The punching cutter 111 is held by the cutter holder 115 except when drilling. The elastic member 103 has a thin film shape. As a result, the height of the container device 118 does not increase compared to the prior art. Therefore, an increase in the size of the container device can be prevented.

It is a schematic perspective view which shows the DNA test | inspection apparatus based on this invention. It is a perspective view which shows the PCR plate which concerns on this invention. It is sectional drawing which shows the container apparatus which concerns on 1st Example of this invention. It is a side view which shows the container apparatus of the state in the middle of the amplification reaction based on 1st Example of this invention. It is sectional drawing which shows the container apparatus of the state just before drilling which concerns on 1st Example of this invention. It is sectional drawing which shows the container apparatus of the state which has the punching cutter which concerns on 1st Example of this invention in the lowest point. It is sectional drawing which shows the container apparatus of the state by which the opening part of the container was closed after completion | finish of the drilling which concerns on 1st Example of this invention. It is sectional drawing which shows a motion of an actuator when opening the opening part of the container which concerns on 1st Example of this invention. It is sectional drawing which shows a motion of an actuator when closing the opening part of the container which concerns on 1st Example of this invention. It is sectional drawing which shows the container apparatus of the state in the middle of the amplification reaction which concerns on 1st Example of this invention. It is sectional drawing which shows the container apparatus which concerns on the 2nd Example of this invention. It is sectional drawing which shows the container apparatus of the state which has the punching cutter which concerns on the 2nd Example of this invention in the lowest point. It is sectional drawing which shows the container apparatus of the state which removes the punching cutter which concerns on the 2nd Example of this invention from a press member. It is sectional drawing which shows one form of the conventional container apparatus. It is sectional drawing which shows another form of the conventional container apparatus.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 DNA test | inspection apparatus 10 Opening part 100,118 Container apparatus 101 Solution 102 Laminate film 103 Elastic member 104 Frame 108, 111 Hole cutter 109 Actuator 110, 114 Container 112 Press member

Claims (8)

A plate having a plurality of openings arranged and having a plurality of containers capable of storing liquids through the openings;
A sealing member which is the first sealing is means for sealing the liquid over each of the plurality of openings individually,
A second sealing means that each of the plurality of openings includes a thin isolation member for covering the sealing member and a holding member for holding the isolation member;
A penetrating means that moves from above the separating member and can break the sealing member through the separating member;
Have
The isolation member is not broken when the penetrating means breaks the sealing member , and can prevent the penetrating means from coming into contact with the sealing member , and the second sealing means Is capable of opening and closing the opening through the holding member .   The container device according to claim 1, wherein the isolation member is an elastic body and is deformably deformed.   The container device according to claim 1 or 2, wherein the penetrating means is composed of a single member, and a tip portion of the penetrating means has a convex shape.   The container device according to claim 1, wherein the penetrating means includes a pressing member and a cutting member, and the cutting member is detachable from the pressing member. Has a pivoting hand stage for rotating the separating member,
The rotating means rotates the separating member with one end of the holding member as a fulcrum;
The container apparatus of any one of Claim 1 to 4. The container device according to any one of claims 1 to 5, further comprising a hinge portion that connects the holding member and the plate.   A DNA test apparatus comprising the container apparatus according to claim 1. Providing a container in which a liquid is contained, an opening is covered with a sealing member, and the sealing member is covered with a thin isolation member held by a holding member ;
A penetrating means moves from above the separating member, and through the separating member breaks the sealing member without breaking the separating member ;
Moving the isolation member through the holding member to open the opening;
Have
The isolation member is not broken when the penetrating means breaks the sealing member, and can prevent the penetrating means from coming into contact with the sealing member.
Method for penetrating container device.

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