JP4959914B2 - Topical skin preparation - Google Patents

Description

  The present invention relates to a skin external preparation containing a carboxylic acid ester of vitamin K hydroquinone or a salt thereof as an active ingredient.

Conventionally, many cosmetics containing an ingredient derived from a natural product as an active ingredient are known. For example, cosmetics containing vitamins are known (see Patent Document 1). However, many components derived from natural products such as vitamins are oily and hardly soluble in water, and there are restrictions on the formulation such as limited dosage forms. On the other hand, a vitamin K derivative having high solubility in water has been proposed (Patent Document 2). However, Patent Document 2 describes that this compound is hydrolyzed in vivo to release vitamin K. However, there is no description on the medicinal effect of this compound itself, There is no description at all about whether or not it shows a medicinal effect as an active ingredient.
JP-A-5-320039 Japanese Patent No. 3088137

  The present invention provides a medicinal agent such as a whitening agent composed of a compound having a specific structure that is highly soluble in water and highly safe, and by using such a medicinal agent, skin exhibiting good medicinal effects It is an object to provide an external preparation, particularly a skin external preparation excellent in whitening effect.

（式中、Ｒ¹及びＲ²はそれぞれ、水素原子、又はアミノ酸、Ｎ−アシルアミノ酸、Ｎ−アルキルアミノ酸、Ｎ，Ｎ−ジアルキルアミノ酸、ピリジンカルボン酸及びそれらの塩の残基から選ばれる窒素置換基を有するカルボン酸残基を意味し、Ｒ¹及びＲ²の少なくとも一方は前記カルボン酸残基である。Ｒ³は水素原子又は下記一般式（２）： In order to solve the above problems, the present invention provides the following general formula (1):

Wherein R ¹ and R ² are each a hydrogen atom or a nitrogen substitution selected from the residues of amino acids, N-acylamino acids, N-alkylamino acids, N, N-dialkylamino acids, pyridinecarboxylic acids and their salts. A carboxylic acid residue having a group, wherein at least one of R ¹ and R ² is the carboxylic acid residue, wherein R ³ is a hydrogen atom or the following general formula (2):

Or the following general formula (3):

  Means a group represented by n means an integer of 1 to 14. The skin external preparation containing at least one kind of carboxylic acid ester of vitamin K hydroquinone represented by (1) or a salt thereof is provided.

  Further, from another viewpoint, the present invention provides an external preparation for skin containing the carboxylic acid ester of vitamin K hydroquinone or a salt thereof as a whitening agent; the carboxylic acid ester of vitamin K hydroquinone or a salt thereof as an antioxidant. Skin external preparation containing; whitening agent comprising carboxylic acid esters of vitamin K hydroquinone or salts thereof; melanin production inhibitor comprising carboxylic acid esters of vitamin K hydroquinone or salts thereof; carboxylic acid esters of vitamin K hydroquinone; Or a salt thereof; a method of using the carboxylic acid ester of vitamin K hydroquinone or a salt thereof as a whitening agent; and a use of the carboxylic acid ester of vitamin K hydroquinone or a salt thereof as an antioxidant. A method.

  Since the carboxylic acid ester of vitamin K hydroquinone represented by the general formula (1) or a salt thereof is highly water-soluble and exhibits an inhibitory effect on melanin production, skin of cosmetics, quasi drugs, pharmaceuticals, etc. It is useful as a whitening component that can be incorporated into a wide range of preparations for external use, and the skin external preparation of the present invention containing this is excellent in whitening effect.

（式中、Ｒ¹及びＲ²はそれぞれ、水素原子、又はアミノ酸、Ｎ−アシルアミノ酸、Ｎ−アルキルアミノ酸、Ｎ，Ｎ−ジアルキルアミノ酸、ピリジンカルボン酸及びそれらの塩の残基から選ばれる窒素置換基を有するカルボン酸残基を意味し、Ｒ¹及びＲ²の少なくとも一方は前記カルボン酸残基である。Ｒ³は水素原子又は下記一般式（２）： Hereinafter, preferred embodiments of the present invention will be described.
The present invention provides the following general formula (1):

Wherein R ¹ and R ² are each a hydrogen atom or a nitrogen substitution selected from the residues of amino acids, N-acylamino acids, N-alkylamino acids, N, N-dialkylamino acids, pyridinecarboxylic acids and their salts. A carboxylic acid residue having a group, wherein at least one of R ¹ and R ² is the carboxylic acid residue, wherein R ³ is a hydrogen atom or the following general formula (2):

Or the following general formula (3):

  Means a group represented by n means an integer of 1 to 14. It is related with the skin external preparation containing the carboxylic acid ester of vitamin K hydroquinone represented by this, or its salt. The compound represented by the general formula (1) or a salt thereof can be contained alone in the skin external preparation, or can be blended in the skin external preparation as a salt thereof.

In the formula, the carboxylic acid residues represented by R ¹ and R ² are selected from the residues of amino acids, N-acyl amino acids, N-alkyl amino acids, N, N-dialkyl amino acids, pyridine carboxylic acids and their salts. In general, the carboxylic acid residue is represented by -C (= O) -R (R is a substituent). In the carboxylic acid residue having a nitrogen substituent, R may include at least a nitrogen substituent, and R may be a nitrogen substituent (for example, an amino group). The nitrogen substituent may be a chain group or a cyclic group. Preferable specific examples include an unsubstituted amino group, an amino group substituted with 1 or 2 alkyl groups or an acyl group, and a pyridyl group. A substituted or unsubstituted amino group is preferred. As the alkyl group of the alkyl-substituted amino group, a linear or branched alkyl group having 1 to 6 carbon atoms such as a methyl group, an ethyl group, an n-propyl group, an n-butyl group, an n-pentyl group, and an n-hexyl group. , An isopropyl group, an isobutyl group, a 1-methylpropyl group, a tert-butyl group, a 1-ethylpropyl group, an isoamyl group, and the like, and a methyl group and an ethyl group are particularly preferable. The acyl group of the acyl-substituted amino group is preferably an acyl group having a hydrocarbon chain of a linear or branched alkyl group having 1 to 6 carbon atoms, and specific examples of the alkyl group moiety are as described above.

  The carboxylic acid residue may contain a linking group that links both the carbonyl group and the nitrogen substituent. The linking group is preferably a linear, branched or cyclic alkylene group having 1 to 7 carbon atoms. The branched alkylene group means an alkylene group derived from an alkyl group such as isopropyl, isobutyl, tert-butyl, 1-ethylpropyl and the like. The cyclic alkylene group means an alkylene group containing a cyclopentane ring, a cyclohexane ring, a methylcyclohexane ring or the like in the structure. Particularly preferred as the alkylene group is a methylene group or an ethylene group.

  The carboxylic acid residue may have a substituent other than a nitrogen substituent. Examples of the substituent other than the nitrogen substituent include a carboxyl group, a hydroxyl group, and a thiol group.

  The nitrogen substituent in the carboxylic acid residue may form a salt, and for example, a hydrohalide, an alkyl sulfonate, and a saccharide are preferable. As the hydrohalide, hydrochloride, hydrobromide and the like are preferable. In the present invention, the hydrohalide salt is often crystallized or solidified, and has an advantage of easy handling in the preparation. Examples of the alkyl sulfonate include methane sulfonate, and examples of the saccharide include gluconate, glucoheptanoate, and lactobionate.

In the present invention, various methods for producing the compound represented by the general formula (1) are conceivable, and typical methods are described as follows. In the following formulae, each of R ^{1 to} R ^{3 has} the same meaning as described above.

  First, vitamin K represented by the general formula (4) is reduced with a reducing agent to form a vitamin K hydroquinone represented by the general formula (5), and this vitamin K hydroquinone and a carboxylic acid having a nitrogen substituent, or The compound represented by the general formula (1) can be produced by esterifying the reactive acid derivative or the hydrohalide thereof with a conventional method. The reducing agent used here is for reducing the naphthoquinone skeleton of vitamin K to naphthohydroquinone skeleton, such as sodium borohydride, hydrosulfite sodium, tri-n-butylphosphine, zinc chloride, stannous chloride, etc. Can be mentioned.

  The esterification reaction between the compound represented by the general formula (5) and a carboxylic acid having a nitrogen substituent can be performed according to a conventional method, and various carboxylic acid residues can be introduced. For example, when producing a compound in which the carboxylic acid residue is a carboxylic acid residue having a primary or secondary amino group, or an amino acid carboxylic acid residue having a hydroxyl group or a thiol group in the side chain, Such a reactive substituent (primary or secondary amino group, hydroxyl group or thiol group) is preferably subjected to esterification reaction using a reagent protected with an appropriate protecting group as a reagent. Examples of the protecting group include tert-butoxycarbonyl group (hereinafter abbreviated as t-BOC group), benzyloxycarbonyl group (hereinafter abbreviated as Z group), 9-fluorenylmethoxycarbonyl group (hereinafter abbreviated as FMOC group) and the like. Can be mentioned. Thereafter, the desired compound can be produced by removing the protecting group as desired.

  In the case of producing a compound in which the carboxylic acid residue is a carboxylic acid residue of an N, N-dialkyl amino acid, an active esterification is carried out using a hydrohalide of N, N-dialkyl amino acid as a reagent. The esterification reaction is preferably performed in the presence of a reagent. Examples of the active esterification reagent include dicyclohexylcarbodiimide (hereinafter abbreviated as DCC), N, N-disuccinimide oxalate (hereinafter abbreviated as DSO), and the like. In this case, anhydrous pyridine is preferred as the solvent. Further, it is also preferable to perform a stealing reaction using a reactive acid derivative of N, N-dialkylamino acid as a reagent. Acid halogenites, especially those using acid chloride, give favorable results. In this case, an anhydrous benzene / anhydrous pyridine mixture is preferred as the solvent.

  A 1,4-dihydroxynaphthoquinone derivative obtained by reacting the free 1,4-dihydroxynaphthoquinone derivative obtained by the above-described method with a lactone body of hydrohalic acid, alkylsulfonic acid or sugar acid, if desired, by a conventional method. The hydrohalide, alkyl sulfonate, or saccharide salt of In addition, when an N-acylamino acid ester of a 1,4-dihydroxynaphthoquinone derivative is produced using an N-acylamino acid as a reagent, it is then halogenated by deprotection with hydrohalic acid by a conventional method. Hydrates can be produced.

The compound represented by the above general formula (1) or a salt thereof of the present invention is an excellent whitening agent that exhibits a remarkable inhibitory action in a melanin production inhibition test using B-16 melanoma cells. Moreover, since the said compound or its salt is highly water-soluble and is soluble in water, it is easy to mix | blend with an aqueous skin external preparation. As a result, it is not necessary to separately add an additive such as a surfactant to solubilize the compound or a salt thereof, which is advantageous in terms of formulation and safety.
In the present specification, the “whitening effect” does not mean only an inhibitory effect on melanin production, but, for example, suppression of pigmentation, skin dullness, prevention and improvement of skin darkening due to sunburn, etc. It is necessary to interpret in the broadest sense including the effects of.

  The skin external preparation of this invention contains the compound or its salt represented by the said General formula (1). The content is preferably 0.00001 to 1% by mass (hereinafter simply referred to as “%”), and more preferably 0.0001 to 0.1%. If it exists in this range, it can mix | blend stably and can show the outstanding medicinal effect.

  Examples of the formulation of the external preparation for skin of the present invention are not particularly limited. For example, emulsions, creams, lotions, cosmetics, packs, cleaning agents, makeup cosmetics, dispersions, ointments, solutions, aerosols, patches Any form of cosmetics such as pills, poultices, liniments and the like may be external medicines.

  The topical skin preparation of the present invention contains components that are usually used in preparations such as cosmetics, quasi-drugs, and external pharmaceuticals, that is, water (purified water), as long as the effects of the present invention are not impaired as necessary. , Hot spring water, deep water, etc.), alcohol, oil agent, surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, UV protection agent, inclusion compound, antibacterial Agent, fragrance, deodorant, salt, pH adjuster, refresher, animal / microbe-derived extract, plant extract, blood circulation promoter, astringent, antiseborrheic agent, whitening agent, anti-inflammatory agent, active oxygen scavenger Agents, cell activators, humectants, chelating agents, keratolytic agents, enzymes, hormones, vitamins and the like can be added. By combining with these drugs, it can be expected to exhibit more excellent effects. Specific examples of suitable components include those shown below. Here, “derivatives” include salts that can be formed.

  Alcohols include lower alcohols such as ethanol, glycerin, diglycerin, ethylene glycol, diethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene for the purpose of dissolution, refreshment, antiseptic, moisturizing, etc. Polyhydric alcohols such as glycol can be used.

As an oil agent, it is a natural oil, a synthetic oil, or a solid oil as long as it is used for normal cosmetics as a constituent component or usability of the base, improving the feeling of use. Regardless of properties such as semi-solid or liquid, hydrocarbons, waxes, fatty acids, higher alcohols, ester oils, silicone oils, fluorine oils and the like can be used.
For example, hydrocarbons such as squalane and petrolatum; olive oil, castor oil, mink oil, macadamia nut oil, apricot oil, persic oil, safflower oil, sunflower oil, avocado oil, medhome oil, camellia oil, almond oil, Oils derived from plants and animals such as sesame oil, sesame oil, borage oil, and shea fat; ester oils such as cetyl isostearate, diglyceryl isostearate and jojoba oil; and waxes such as beeswax, carnauba wax, candelilla wax, and gay wax Is mentioned.

  Examples of UV protection agents include para-methoxycinnamate-2-ethylhexyl, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 sodium sulfate, 4-t-butyl-4′-methoxydi Examples include benzoylmethane, 2-phenyl-benzimidazole-5-sulfuric acid, titanium oxide, and zinc oxide.

  The water-soluble polymer is used for stabilizing the system, improving the usability and the feeling of use, and is also used for obtaining a moisturizing effect. Specific examples of water-soluble polymers include plant polymers such as carrageenan, pectin, agar, locust bean gum, microbial polymers such as xanthan gum, animal polymers such as casein and gelatin, and starch polymers such as starch. And cellulose polymers such as methyl cellulose, ethyl cellulose, carboxymethyl cellulose, hydroxypropyl cellulose, and crystalline cellulose, alginic acid polymers such as sodium alginate, vinyl polymers such as carboxyvinyl polymer, and the like.

  Examples of the antibacterial agent include benzoic acid, sodium benzoate, paraoxybenzoic acid ester, benzalkonium chloride, phenoxyethanol, isopropylmethylphenol and the like.

  The whitening agent is used for the purpose of preventing skin darkening caused by sunburn, etc., stains caused by pigmentation, freckles, etc., vitamin C and its derivatives, placenta extract, licorice extract, age extract, ougon extract , Seaweed extract, cucumber extract, caquette extract, gokahi extract, rice bran extract, wheat germ extract, saicin extract, hawthorn extract, sun penz extract, shirayuri extract, peony extract, sempukuka extract , Soybean extract, tea extract, molasses extract, juniper extract, grape extract, hop extract, micaika extract, mokka extract, yukinoshita extract, yokuinin extract and the like.

  Anti-inflammatory agents are used for the purpose of suppressing inflammation such as hot flashes and erythema on the skin after sunburn. Sulfur and its derivatives, glycyrrhizic acid and its derivatives, glycyrrhetinic acid and its derivatives, aloe extract, Altea extract, Ashitaba Extract, Arnica extract, Ginseng extract, Nettle extract, Duckweed extract, Hypericum extract, Chamomile extract, Snapdragon extract, Watercress extract, Comfrey extract, Salvia extract, Shikon extract, Perilla extract Product, birch extract, gentian extract and the like.

  Cell activators are used for the purpose of improving rough skin, etc., such as caffeine, chicken crown extract, shell extract, shell extract, royal jelly, silk protein and its degradation product or derivatives thereof, lactoferrin or its degradation product, Mucopolysaccharides such as chondroitin sulfate and hyaluronic acid, or salts thereof, collagen, yeast extract, lactic acid bacteria extract, bifidobacteria extract, fermentation metabolic extract, ginkgo biloba extract, barley extract, assembly extract, tiso extract , Carrot extract, rosemary extract, glycolic acid, citric acid, lactic acid, malic acid, tartaric acid, organic acids such as succinic acid, and derivatives thereof.

  Active oxygen scavenger is used for the purpose of suppressing oxidative damage such as lipid peroxide production suppression, superoxide dismutase, mannitol, quercetin, catechin and its derivatives, rutin and its derivatives, button pi extract, yashajitsu extract, melissa extract , Extract of rahan fruit, retinol and its derivatives, vitamin A such as carotenoid, thiamine and its derivative, riboflavin and its derivative, pyridoxine and its derivative, vitamin B such as nicotinic acid and its derivative, tocopherol and its derivative, etc. Vitamin E, dibutylhydroxytoluene, butylhydroxyanisole, etc. are mentioned.

  As the humectant, proteins such as elastin and keratin or derivatives thereof, hydrolysates and salts thereof, amino acids such as glycine, serine, aspartic acid, glutamic acid, arginine and theanine and derivatives thereof, sorbitol, erythritol, trehalose, Inositol, glucose, sucrose and derivatives thereof, dextrin and derivatives thereof, saccharides such as honey, D-panthenol and derivatives thereof, urea, phospholipids, ceramide, auren extract, agate extract, agar extract, a senkyu extract, Examples of this include mallow mushroom extract, periwinkle extract, dokudami extract, hamamelis extract, bodaige extract, maronier extract, quince extract and the like.

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. However, the scope of the present invention is not limited to the following examples.
[Synthesis Example] Compound Nos. Shown in Tables 1 to 5 by any one of the following production methods A to F were used. 1-28 were produced. Moreover, the measurement results of the mass spectrum (ionization method; FD method and FAB method) and ¹ H-NMR spectrum of the obtained substance are shown in Tables 6-8.

<< Production Method A >>
Amino acid (for example, glycine in the case of producing Compound No. 1 in Table 1) 0.1 mol was dissolved in 100 ml of distilled water-dioxane (1: 1 v / v), 30 ml of triethylamine was added, and di-tert-butyldicarb was added. Slowly add the bonate and stir for 30 minutes at room temperature. Dioxane is distilled off under reduced pressure, and 50 ml of an aqueous sodium hydrogen carbonate solution (0.5 M) is added, followed by washing with 100 ml of ethyl acetate. The ethyl acetate layer was washed with 50 ml of sodium hydrogen carbonate solution, and the aqueous layers were combined and acidified (pH 3) with an aqueous citric acid solution (0.5 M) under ice-cooling. After saturating sodium chloride, ethyl acetate was added. Extract (100 ml × 3 times). The extract is dehydrated with anhydrous sodium sulfate, the solvent is distilled off under reduced pressure, and the oily residue is crystallized by adding isopropyl ether or cooling to give Nt-BOC. -Obtain amino acids. Dissolve 6.75 mmol of vitamin K in 40 ml of isopropyl ether, add 47 mmol of sodium borohydride dissolved in 15 ml of methanol and stir at room temperature until the yellow color of the solution becomes colorless. 60 ml of isopropyl ether and 100 ml of distilled water are added to the reaction solution, the isopropyl ether layer is separated, 100 ml of isopropyl ether is further added to the aqueous layer, and the soluble fraction is extracted, and the isopropyl ether layers are combined and washed with anhydrous sodium sulfate. After dehydration, concentrate under reduced pressure. N-hexane is added to the residue to precipitate a white precipitate to obtain vitamin K hydroquinone.

  Vitamin K hydroquinone (6.75 mmol), Nt-BOC-amino acid 13.55 mmol and DCC 13.55 mmol are added to 50 ml of anhydrous pyridine and stirred at room temperature for 20 hours. The solvent was distilled off under reduced pressure, ethyl acetate was added to the residue to extract a soluble fraction (100 ml × 2 times), the extract was concentrated under reduced pressure, and the residue was subjected to silica gel column chromatography (eluent: n-hexane). -Isopropyl ether) to obtain vitamin K hydroquinone-1,4-bis-Nt-BOC-amino acid. Vitamin K hydroquinone-1,4-bis-Nt-BOC-amino acid is dissolved in a small amount of acetone, and hydrochloric acid-dioxane (2.5 to 4.0 N) is adjusted to a hydrochloric acid amount of about 20 times molar amount of the ester amount. The corresponding amount was added and stirred for 1 hour, and then the solvent was distilled off under reduced pressure. The residue is recrystallized in an acetone-methanol system to obtain hydrochloride salt of vitamin K hydroquinone-1,4-bis-amino acid ester.

<< Production Method B >>
Vitamin K (6.75 mmol) is dissolved in 40 ml of isopropyl ether, 47 mmol of sodium borohydride is dissolved in 15 ml of methanol and stirred at room temperature until the yellow color of the solution becomes colorless. Add 60 ml of isopropyl ether and 100 ml of distilled water to the reaction solution, separate the isopropyl ether layer, add 100 ml of isopropyl ether to the aqueous layer, extract the soluble fraction, combine the isopropyl ether layers and dehydrate with anhydrous sodium sulfate. Then, it is concentrated under reduced pressure. N-hexane is added to the residue to precipitate a white precipitate to obtain vitamin K hydroquinone. Vitamin K hydroquinone (6.75 mmol), N, N-dialkylamino acid hydrochloride (for example, N, N-dimethylglycine is used when producing compound No. 7 in Table 2) and DCC 13.55 mmol Is added to 50 ml of anhydrous pyridine and stirred at room temperature for 20 hours. The solvent was distilled off under reduced pressure, the residue was suspended in distilled water and sodium bicarbonate was added to adjust the pH of the solution to 7-8, followed by extraction with ethyl acetate (100 ml × 3 times). After dehydration with sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was separated and purified by silica gel column chromatography (eluent: isopropyl ether-ethyl acetate), and vitamin K hydroquinone-1,4-bis-N, N-dialkyl. An amino acid ester is obtained.

<< Production Method C >>
Vitamin K (6.75 mmol) is dissolved in 40 ml of isopropyl ether, 50 mmol of hydrosulfite sodium is dissolved in 50 ml of distilled water, and the mixture is stirred at room temperature until the isopropyl ether becomes brown and further colorless. The isopropyl ether layer is separated, and 100 ml of isopropyl ether is added to the aqueous layer to extract a soluble fraction. The isopropyl ether layers are combined, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. N-hexane is added to the residue to precipitate a white precipitate to obtain vitamin K hydroquinone. Vitamin K hydroquinone (6.75 mmol) with N, N-dialkylamino acid hydrochloride (for example, N, N-dimethylglycine is used when producing compounds No. 8 and No. 9 in Table 2) and DCC 6.75 mmol is added and stirred in 50 ml of anhydrous pyridine for 20 hours. The solvent is distilled off under reduced pressure, the residue is suspended in distilled water, sodium bicarbonate is added to adjust the pH of the solution to 7-8, and the mixture is extracted with ethyl acetate (100 ml × 3 times). The extract was dehydrated with anhydrous sodium sulfate, the solvent was distilled off under reduced pressure, and the residue was separated and purified by silica gel column chromatography (elution solvent; isopropyl ether-ethyl acetate = 3: 2), and vitamin K hydroquinone-1-N. N-dialkyl amino acid ester and vitamin K hydroquinone-4-N, N-dialkyl amino acid ester are obtained.

<< Production Method D >>
Dissolve 6.75 mmol of vitamin K in 40 ml of isopropyl ether, add 47 mmol of sodium borohydride dissolved in 15 ml of methanol and stir at room temperature until the yellow color of the solution becomes colorless. 60 ml of isopropyl ether and 100 ml of distilled water are added to the reaction solution, the isopropyl ether layer is separated, 100 ml of isopropyl ether is further added to the aqueous layer, and the soluble fraction is extracted. The isopropyl ether layers are combined and washed with anhydrous sodium sulfate. After dehydration, concentrate under reduced pressure. N-hexane is added to the residue to precipitate a white precipitate to obtain vitamin K hydroquinone. Vitamin K hydroquinone is dissolved in 30 ml of anhydrous benzene-anhydrous pyridine (1: 1, v / v), pyridinecarboxylic acid chloride hydrochloride is added, and the mixture is stirred at room temperature for 3 hours. Insoluble materials are removed by filtration, and the filtrate is concentrated under reduced pressure. The residue is suspended in 100 ml of distilled water, sodium hydrogen carbonate is added (pH 7-8), and a soluble fraction is extracted in ethyl acetate (100 ml × 3 times). The extract is concentrated under reduced pressure, and the residue is separated and purified by silica gel column chromatography (eluent: isopropyl ether-ethyl acetate, 9: 1) to obtain vitamin K hydroquinone-1,4-bis-pyridinecarboxylic acid ester.

<< Production Method E >>
2 mmol of vitamin K hydroquinone-1,4-bis-N, N-dialkylamino acid ester or vitamin K hydroquinone-1,4-bis-pyridinecarboxylic acid is dissolved in 20 ml of acetone, and hydrochloric acid-dioxane (2.5 to 4.0 N) is dissolved. ) Is added in an amount corresponding to 10 times the molar amount of the ester, the solvent is distilled off under reduced pressure, and the residue is recrystallized from acetone-methanol to give vitamin K hydroquinone-1,4-bis-N, N-dialkyl. A hydrochloride salt of amino acid or vitamin K hydroquinone-1,4-bis-pyridinecarboxylic acid is obtained.

<< Production Method F >>
2 mmol of vitamin K hydroquinone-1,4-bis-N, N-dialkylamino acid or vitamin K hydroquinone-1,4-bis-pyridinecarboxylic acid is dissolved in 20 ml of dichloromethane, and 2 mmol of alkylsulfonic acid is added and stirred. The precipitated crystals are collected by filtration to obtain an alkyl sulfonate of vitamin K hydroquinone-1,4-bis-N, N-dialkyl amino acid ester or vitamin K hydroquinone-1,4-bis-pyridinecarboxylic acid ester.

The synthesized compound No. 24 and compound no. The melanin production inhibitory effect on the cultured pigment cells of the samples containing 28 each (samples 1 and 2) was evaluated as follows.
[Example 1 Inhibition test of melanin production by cultured cells]
The cultured cells were mouse-derived B-16 melanoma cells. Compound No. produced above 24 and no. Only 28 purified aqueous solutions and purified water were used as samples added to the medium. The medium was changed halfway, and after culturing for 5 days, the cells were collected, the number of cells was measured, and intracellular melanin was quantified. Similarly, the amount of melanin at the concentration of each sample was quantified by setting the amount of melanin when purified water was added instead of the sample as 100%, and was defined as the melanin production rate (%). In order to evaluate the melanin production inhibitory effect of the sample, IC50 was introduced as an evaluation standard. Specifically, the sample concentration IC50 (melanin production rate) at which the melanin production rate was 50% was determined. The smaller the value of IC50 (melanin production rate), the higher the melanin production inhibitory effect.

  As Comparative Sample 1, L-ascorbic acid magnesium phosphate, which is a water-soluble compound that is generally known to have a melanin production inhibitory effect, was used for the same test as described above. Table 50 shows IC50 (melanin production rate) obtained for Samples 1 and 2 of the present invention and Comparative Sample 1.

  From the results shown in Table 9, it was revealed that Samples 1 and 2 of the present invention were significantly superior to Comparative Sample 1 in the melanin production inhibitory effect. From the above, it was clarified that the product of the present invention is remarkably superior in whitening effect as compared with the conventional water-soluble whitening agent.

Examples of the external preparation for skin containing the compound of the general formula (1) synthesized in the above synthesis examples will be shown below.
[Example 2: lotion]
A solution in which the following components (3) to (5) and (9) to (11) are mixed and dissolved, and a solution in which the components (1), (2), (6) to (8) and (12) are mixed and dissolved Were mixed to obtain a skin lotion.
(Prescription) (%)
(1) Glycerin 5.0
(2) 1,3-butylene glycol 6.5
(3) Polyoxyethylene (20E.O.) sorbitan 1.2
Monolaurate (4) Ethyl alcohol 8.0
(5) Compound No. 24 0.005
(6) Magnesium L-ascorbate phosphate * 1 0.5
(7) Lactic acid 0.05
(8) Sodium lactate 0.1
(9) Paramethoxycinnamic acid-2-ethylhexyl 3.0
(10) Preservative appropriate amount (11) perfume appropriate amount (12) remaining amount of purified water * 1 Sigma

[Example 3: Latex]
The following components (1) to (6), (8) and (9) were heated and mixed, and the mixture maintained at 70 ° C was heated and mixed with components (12), (13) and (16) to 70 ° C. In addition to the maintained mixture, mixed and uniformly emulsified. After the mixture was cooled, the mixture of components (7), (10) and (15) was added and mixed uniformly. Component (11) was added to this mixture and stirred sufficiently, and then components (14) and (17) were added and mixed uniformly to obtain an emulsion.
(Prescription) (%)
(1) Polyoxyethylene (10E.O.) sorbitan 1.0
Monostearate (2) Polyoxyethylene (60EO) Sorbit 0.5
Tetraoleate (3) Glyceryl monostearate 1.0
(4) Stearic acid 0.5
(5) Behenyl alcohol 0.5
(6) Squalane 8.0
(7) Retinol palmitate * 1 0.002
(8) Dipotassium glycyrrhizinate * 2 0.3
(9) Compound No. 10 0.02
(10) Daylily extract * 3 0.1
(11) Hyaluronic acid 0.1
(12) Preservative 0.1
(13) Carboxyvinyl polymer 0.1
(14) Sodium hydroxide 0.05
(15) Ethyl alcohol 5.0
(16) Purified water remaining amount (17) Fragrance appropriate amount * 1 Made by Nippon Roche * 2 Made by Sigma * 3 Made by Maruzen Pharmaceutical Co., Ltd.

  Both the lotion of Example 2 and the emulsion of Example 3 were highly safe and excellent in the whitening effect.

Claims (2)

The following general formula (1):

Wherein R ¹ and R ² are each a hydrogen atom or a nitrogen substitution selected from the residues of amino acids, N-acylamino acids, N-alkylamino acids, N, N-dialkylamino acids, pyridinecarboxylic acids and their salts. A carboxylic acid residue having a group, and at least one of R ¹ and R ² is the carboxylic acid residue. R ³ represents a hydrogen atom.) Or the skin external preparation containing at least 1 sort (s) of the salt. The skin external preparation of Claim 1 which contains the carboxylic acid ester of vitamin K hydroquinone represented by the said General formula (1), or its salt as a whitening agent.