JP4923951B2 - Evaluation method of rust - Google Patents
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- JP4923951B2 JP4923951B2 JP2006291403A JP2006291403A JP4923951B2 JP 4923951 B2 JP4923951 B2 JP 4923951B2 JP 2006291403 A JP2006291403 A JP 2006291403A JP 2006291403 A JP2006291403 A JP 2006291403A JP 4923951 B2 JP4923951 B2 JP 4923951B2
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Description
本発明は、二価の鉄イオン由来の不快な錆味の評価方法に関する。さらに詳しくは、二価の鉄イオンに表皮脂質成分を配合することを特徴とする錆味の評価方法に関する。 The present invention relates to a method for evaluating unpleasant rust derived from divalent iron ions. More specifically, the present invention relates to a method for evaluating rust, which comprises blending an epidermal lipid component with divalent iron ions.
従来から、二価の鉄イオンは三価の鉄イオンに比べて吸収性がよいことが知られているが、二価の鉄イオンには不快な錆味があり、二価の鉄イオンを配合して内服液剤を調製した場合、服用性が著しく悪くなるという問題があった。 Traditionally, it has been known that divalent iron ions have better absorbability than trivalent iron ions, but divalent iron ions have an unpleasant rust and contain divalent iron ions. Then, when the internal liquid preparation was prepared, there was a problem that the ingestibility was remarkably deteriorated.
従来、飲料の風味を評価する方法としては、渋味を有する食品とペプチドとを反応させ複合体の生成量で渋味を評価する方法（特許文献１）、高分子材などで味の成分を分離し機械的に渋味や苦味を評価する方法（特許文献２、３）などが知られているが、二価の鉄イオン由来の錆味を評価する方法としては、服用による官能評価しかないのが現状である。しかしながら官能評価の場合、連続して評価することが困難であり、通常一度評価を行った後、３０分程度間隔を空けないと次の評価を行うことができないなど、必ずしも簡便とは言えなかった。
Conventionally, as a method of evaluating the flavor of a beverage, a food product having astringency and a peptide are reacted with each other to evaluate the astringency by the amount of complex produced (Patent Document 1). Methods for separating and mechanically evaluating astringency and bitterness (
また、ヒトの表皮脂質の組成はおよそ平均値でトリアシルグリセリン４０％、脂肪酸１５％、スクアレン１０％、ワックス２５％程度であることが知られている（非特許文献1）。 In addition, it is known that the composition of human epidermal lipids is approximately 40% triacylglycerol, 15% fatty acid, 10% squalene, and 25% wax (average 1) (Non-Patent Document 1).
本発明の目的は、二価の鉄イオン由来の不快な錆味を簡便に評価する方法を提供することにある。 An object of the present invention is to provide a method for simply evaluating an unpleasant rust derived from divalent iron ions.
上記課題を解決するために、本発明者らは鋭意検討を重ねた結果、二価の鉄イオンを服用すると不快な錆味を感じるが、これは溶液のままでは感じず、一旦唇や皮膚に付着すると感じることを見出した。さらにその原因を探求したところ、二価の鉄イオン由来の不快な錆味は表皮脂質成分と接触することにより発現することが分かった。そのことから、二価の鉄イオン配合液剤に表皮脂質成分を添加することにより発生する臭いから錆味の評価ができることを見出し本発明を完成した。 In order to solve the above-mentioned problems, the present inventors have conducted intensive studies, and as a result, when they take divalent iron ions, they feel unpleasant rust, but they do not feel as they are in the solution, but once on the lips and skin. I found that I feel that it adheres. Furthermore, when the cause was investigated, it turned out that the unpleasant rust derived from a bivalent iron ion expresses by contacting with an epidermal lipid component. Thus, the inventors found that rust can be evaluated from the odor generated by adding an epidermal lipid component to a divalent iron ion compounding liquid, thereby completing the present invention.
すなわち本発明は
（１）鉄成分配合液剤に表皮脂質成分を添加し、発生する臭いを評価することを特徴とする鉄成分配合液剤における錆味の評価方法。
（２）鉄成分が二価の鉄成分であることを特徴とする（１）に記載の評価方法。
（３）表皮脂質成分がスクアレンである（１）又は（２）に記載の評価方法。
である。
That is, the present invention is (1) a method for evaluating rust in an iron component-containing liquid agent, wherein an epidermal lipid component is added to the iron component-containing liquid agent and the generated odor is evaluated.
(2) The evaluation method according to (1), wherein the iron component is a divalent iron component.
(3) The evaluation method according to (1) or (2), wherein the epidermal lipid component is squalene.
It is.
本発明により、二価の鉄イオン由来の不快な錆味を簡便に評価することができた。 By this invention, the unpleasant rust derived from a bivalent iron ion was able to be evaluated easily.
本発明で鉄成分とは液剤に溶解した際に鉄イオンを生じる成分である。鉄成分としては二価の鉄イオンを生じる成分が好ましいが、三価の鉄化合物と還元性物質を共存させた場合でも錆味が発生するため本発明で評価することができる。二価の鉄イオンを生じる成分としてはフマル酸第一鉄、硫酸第一鉄、クエン酸第一鉄ナトリウムなどが挙げられる。また、三価の鉄化合物と還元性物質を共存させる場合の還元性物質としては還元糖、アスコルビン酸などの還元性有機酸などが挙げられる。これらは単独で配合してもよく、また２種以上を組み合わせて配合することもできる。 In the present invention, the iron component is a component that generates iron ions when dissolved in a liquid. As the iron component, a component that generates divalent iron ions is preferable. However, even when a trivalent iron compound and a reducing substance coexist, rust is generated, which can be evaluated in the present invention. Examples of components that generate divalent iron ions include ferrous fumarate, ferrous sulfate, and sodium ferrous citrate. Examples of the reducing substance in the case where the trivalent iron compound and the reducing substance coexist include reducing organic acids such as reducing sugar and ascorbic acid. These may be blended singly or in combination of two or more.
本発明で表皮脂質成分としてはスクアレンが最も好ましい。本発明に使用するスクアレンとは、イソプレン残基６個からなる鎖状構造を持つイソプレン系化合物であり、多くのサメ、特にフジクジラ、カラスザメ、カスミザメなどの深海性のサメの肝油の不ケン化物や諸動物の肝臓や人の皮脂、オリーブ油、ゴマ油、小麦胚油、米糠油などに存在し、それらより分離精製された不飽和炭化水素である。なおこれらのスクアレン以外に合成品を使用することもできる。 In the present invention, squalene is the most preferred epidermal lipid component. The squalene used in the present invention is an isoprene-based compound having a chain structure composed of six isoprene residues, and is an unsaponified product of liver oil of many sharks, particularly deep-sea sharks such as whales, crow sharks and shark sharks. It is an unsaturated hydrocarbon that is present in the livers of various animals, human sebum, olive oil, sesame oil, wheat germ oil, rice bran oil, etc., separated and purified from them. In addition to these squalenes, synthetic products can also be used.
本発明は、鉄成分配合液剤に表皮脂質成分を添加し、発生する臭いを評価する。臭いの評価は、臭いを官能的に評価するのが最も簡便であるが、ガスクロマトグラフィーなどを用いて機械的に評価することも可能である。臭いを官能的に評価する場合、従来行われていた服用しての評価と異なり、嗅覚を用いて評価できるので連続して多くのサンプルを評価することが可能である。 In the present invention, an epidermal lipid component is added to an iron component-containing liquid preparation, and the generated odor is evaluated. For the evaluation of odor, it is easiest to sensuously evaluate the odor, but it is also possible to perform mechanical evaluation using gas chromatography or the like. When the odor is evaluated sensuously, it is possible to evaluate using the sense of smell unlike the conventional evaluation by taking the odor, so that it is possible to evaluate many samples continuously.
本発明の錆味の評価方法を使用して、例えば鉄剤を配合したシロップ剤、ドリンク剤などの医薬品や医薬部外品などの各種製剤、健康飲料などの各種飲料の錆味の評価を行なうことができる。 Using the rust evaluation method of the present invention, for example, evaluating rust of various beverages such as various preparations such as pharmaceuticals and quasi-drugs such as syrups and drinks containing iron preparations and quasi-drugs Can do.
以下、実施例により本発明をさらに詳しく説明する。 Hereinafter, the present invention will be described in more detail with reference to examples.
表皮脂質の構成成分の内、不飽和結合を有することから反応性が高いことが予想されるスクアレン、オレイン酸、パルミトレイン酸について検討を行った。 Among the constituents of epidermal lipids, squalene, oleic acid, and palmitoleic acid, which are expected to be highly reactive due to having an unsaturated bond, were examined.
試験方法
（1）希釈液の調製
クエン酸100mgを適量の水に溶解し、1mol/L水酸化ナトリウムでpHを3.0に調整し、精製水で100mLとした。
（2）鉄イオン溶液の調製
フマル酸第一鉄150mgにクエン酸100mgを加え、それを適量の精製水に溶解し、1mol/L塩酸及び1mol/L水酸化ナトリウムでpHを3.0に調整し、精製水で100mLとした。
（3）ドデシル硫酸ナトリウム（SDS）溶液の調製
SDS 10gにクエン酸100mgを加え、それを適量の精製水に溶解し、1mol/L塩酸及び1mol/L水酸化ナトリウムでpHを3.0に調整し、精製水で100mLとした。
（4）SDS-脂質可溶化溶液の調製
（3）項のSDS溶液30mLに、各脂質（スクアレン、オレイン酸、パルミトレイン酸）0.15mLを添加し、30分間攪拌した後30分間静置し、各SDS-脂質可溶化溶液を得た。
（5）錆味の評価
（2）項の鉄イオン溶液5mLにSDS-脂質可溶化溶液0.2mLを加え、希釈液でそれぞれ全量を22mLとした。これを5分間静置した後、サンプルの臭いについて専門パネル４名により嗅覚による官能評価を行った。なお官能評価は、錆味（臭い）が強く許容できない場合を6点、許容できる範囲をその錆味の強さに応じて5点〜2点とし、錆味を全く感じない場合を1点とする6段階の評価点で行った。
Test Method (1) Preparation of Diluent 100 mg of citric acid was dissolved in an appropriate amount of water, pH was adjusted to 3.0 with 1 mol / L sodium hydroxide, and made up to 100 mL with purified water.
(2) Preparation of iron ion solution Add 100 mg of citric acid to 150 mg of ferrous fumarate, dissolve it in an appropriate amount of purified water, adjust the pH to 3.0 with 1 mol / L hydrochloric acid and 1 mol / L sodium hydroxide, Made up to 100 mL with purified water.
(3) Preparation of sodium dodecyl sulfate (SDS) solution
To 10 g of SDS, 100 mg of citric acid was added, dissolved in an appropriate amount of purified water, adjusted to pH 3.0 with 1 mol / L hydrochloric acid and 1 mol / L sodium hydroxide, and adjusted to 100 mL with purified water.
(4) Preparation of SDS-lipid solubilization solution Add 0.15 mL of each lipid (squalene, oleic acid, palmitoleic acid) to 30 mL of the SDS solution in (3), stir for 30 minutes, and leave for 30 minutes. An SDS-lipid solubilized solution was obtained.
(5) Evaluation of rustiness 0.2 mL of SDS-lipid solubilizing solution was added to 5 mL of the iron ion solution described in (2), and the total volume was adjusted to 22 mL with the diluted solution. After leaving this still for 5 minutes, the sensory evaluation by the sense of smell was performed by four special panels about the smell of the sample. The sensory evaluation is 6 points when the rust (odor) is strongly unacceptable, 5 to 2 points depending on the strength of the rust, and 1 point when there is no rust. It was done with 6 grades.
結果
結果を平均値で求め、図１に示した。図１から明らかなように、表皮脂質成分であるスクアレンに二価の鉄イオン溶液を添加したときに、二価の鉄イオンを服用した時と同じような臭いを確認することができた。なお、オレイン酸及びパルミトレイン酸については、油の酸化臭であり、錆味とは質の異なる臭いであった。
Results The results were obtained as average values and are shown in FIG. As can be seen from FIG. 1, when a divalent iron ion solution was added to squalene, which is an epidermal lipid component, the same odor as when taking divalent iron ions could be confirmed. In addition, about oleic acid and palmitoleic acid, it was an oxidation odor of oil, and it had a different odor from rust.
よって錆味の発生原因の一つとして表皮脂質成分であるスクアレンが示唆され、スクアレンを用いた錆味を擬似的に再現可能な評価方法を確立することができた。 Therefore, squalene, an epidermal lipid component, was suggested as one of the causes of rust, and an evaluation method capable of reproducing rust using squalene in a pseudo manner could be established.
本発明の評価方法により、液剤の錆味を簡便に評価できるので、鉄成分を配合したシロップ剤、ドリンク剤などの医薬品や医薬部外品などの各種製剤、健康飲料などの各種飲料の風味検討に使用することができる。 The evaluation method of the present invention makes it possible to easily evaluate the rust of a liquid preparation, so that it can be used to examine the flavor of various beverages such as pharmaceutical preparations such as syrups, drinks, and quasi-drugs, and health drinks. Can be used for
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| JP4923951B2 true JP4923951B2 (en) | 2012-04-25 |
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