JP4868619B2 - Edible plant containing high concentration of vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine and method for producing the same - Google Patents

Description

  The present invention relates to an edible plant body containing vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine at a high concentration and a method for producing the edible plant body, and more specifically, an edible plant body under specific conditions. The present invention relates to a method for efficiently producing an edible plant containing a high concentration of these substances by treating with a solution containing vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine.

  It is known that specific amino acids and peptides have various nutritional and physiological functions. In particular, recently, the amino acids cysteine, γ-aminobutyric acid (GABA), L-carnitine, vitamin U, dipeptide carnosine, anserine, valinyltyrosine, tyrosinylproline, tripeptide glutamylcysteinylglycine (GSH). Physiological function is attracting attention. Thus, although it has been found that specific amino acids and peptides have various functions, edible plants containing these substances in high concentrations do not exist in nature. Therefore, in order to take a certain amount of these substances, there is currently no method other than “taken as a so-called supplement or medicine”. However, the Japanese food culture is different from that of developed countries such as the United States, in which functional substances are valued by “taken by ordinary meals”. Therefore, ingesting functional substances from live vegetables such as sprout rather than so-called supplements and medicines has great significance.

  From such a background, if a plant containing specific amino acids and peptides can be easily produced, the social needs are considered to be great. However, as described above, cultivated plants containing high concentrations of specific amino acids and peptides have not been cultivated at present, and there is almost no research on their production.

  JP-A-6-57288 (Patent Document 1) and JP-A-2003-9666 (Patent Document 2) have been known as conventional techniques relating to the absorption of plant amino acids and peptides. The former aims to promote the rooting of plants by applying a sulfur-containing amino acid or the like by applying a sulfur-containing amino acid, etc. No mention is made regarding the enhancement of the content of specific amino acids and peptides in the plant, and no data is shown on how much the concentration in the plant fluctuates due to absorption of amino acids and peptides.

  Further, JP-A-2004-065240 (Patent Document 3) discloses that a plant or the like absorbs vitamin B12, but the substance to be treated is limited to vitamin B12, and a specific amino acid in the plant No mention is made regarding the enhancement of the content of the peptides and peptides, and no data is shown on how much the accumulated content in the plant varies due to the absorption of amino acids and peptides.

  JP, 2002-305998, A (patent documents 4) discloses accumulating amino acids and the like in plants, but is intended to change the taste by treatment, and GABA, vitamin U, taurine, carnosine, Tyr No mention is made of -Pro and L-carnitine. Further, no data is shown regarding the setting of conditions for absorption treatment and how much the accumulated content in the plant varies.

JP-A-6-57288 JP2003-9666 JP 2004-065240 A Japanese Patent Laid-Open No. 2002-305998

  Under such circumstances, the prior art includes technologies corresponding to edible plants containing GABA, vitamin U, taurine, carnosine, Tyr-Pro and L-carnitine at high concentrations, and methods for producing these edible plants. There is no such plant. Therefore, the development of an edible plant body containing GABA, vitamin U, and taurine in high concentrations in a natural form is considered to be very significant.

  Therefore, an object of the present invention is to provide edible plants containing GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine at a high concentration (for example, 10 mg / 100 g FW or more) and edible plants. It is to provide a manufacturing method.

  Under such circumstances, the present inventors easily produce a plant body containing a high concentration of GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine in view of the above prior art. We have earnestly researched about the development of technology. As a result, these substances are immersed in a specific GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine solution at a certain concentration or higher for a certain period of time under special conditions. The present inventors have found that a plant containing a high concentration of can be produced, and have further researched to complete the present invention.

The present invention is as follows.
[1] A sprout having a vitamin U content of 10 mg / 100 g FW or more, which is manufactured and accumulated by a method in which the sprout is immersed in an aqueous solution containing vitamin U and the vitamin U is accumulated in the sprout. Sprout with excellent storage stability of vitamin U.
[2] The sprout according to [ 1 ], wherein the vitamin U content is 70 mg / 100 g FW or more.
[3] A sprout having a taurine content of 20 mg / 100 g FW or more, which is produced by immersing the sprout in an aqueous solution containing taurine, and accumulating and containing the taurine in the sprout, and storing the accumulated taurine Sprout with excellent stability .
[4] The sprout according to [3], wherein the taurine content is 60 mg / 100 g FW or more.
[5] Storage of stored carnosine, which is a sprout having a carnosine content of 15 mg / 100 g FW or more, which is produced by immersing the sprout in an aqueous solution containing carnosine, and accumulating the carnosine in the sprout. Sprout with excellent stability .
[6] The sprout according to [5], wherein the carnosine content is 50 mg / 100 g FW or more.
[7] A sprout having a Tyr-Pro content of 3 mg / 100 g FW or more , wherein the sprout is immersed in an aqueous solution containing Tyr-Pro, and the Tyr-Pro is accumulated and contained in the sprout; and Sprout excellent in storage stability of accumulated Tyr-Pro .
[8] The sprout according to [7], wherein the Tyr-Pro content is 10 mg / 100 gFW or more.
[9] A sprout having an L-carnitine content of 15 mg / 100 g FW or more , wherein the sprout is immersed in an aqueous solution containing L-carnitine, and the L-carnitine is accumulated and contained in the sprout, and A sprout with excellent storage stability of accumulated L-carnitine .
[10] The sprout according to [9], wherein the L-carnitine content is 50 mg / 100 g FW or more.
[ 11 ] The substance is prepared by immersing a sprout in an aqueous solution containing at least one substance selected from the group consisting of vitamin U, taurine, carnosine, Tyr-Pro and L-carnitine, and accumulating the substance in the sprout. And the accumulated substance is a method for producing a sprout having excellent storage stability ,
The Sprout roots were immersed at all times or intermittently to the aqueous solution, other than the root always or intermittently maintained out of the aqueous solution so as to transpiration,
The sprout and aqueous solution are maintained at 20-35 ° C.,
A method for obtaining a sprout containing at least 10 mg / 100 g FW or more of the above substance.
[ 12 ] The method according to [ 11 ], wherein the sprout is brought into contact with the aqueous solution in a rotating container.
[ 13 ] The method according to [ 11 ], wherein a sprout is brought into contact with the aqueous solution in a container in which the sprout is placed.
[ 14 ] The method according to any one of [ 11 ] to [ 13 ], wherein the sprout is immersed in the aqueous solution for 6 to 24 hours.
[15]
The method according to any one of [11] to [14], wherein the substance is at least one substance selected from the group consisting of vitamin U and taurine.
[16]
The method according to any one of [11] to [14], wherein the substance is at least one substance selected from the group consisting of carnosine, Tyr-Pro, and L-carnitine.

  According to the present invention, an edible plant containing vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine at a high concentration in a natural form can be provided.

The experimental result of GABA of Example 1. The experimental result of vitamin U of Example 1. The experimental result of the taurine of Example 1. FIG. The experimental result of Example 2. FIG. The experimental result of Example 3. FIG. The experimental result of Example 4. FIG. The experimental result of Example 5. FIG. The experimental result of Example 6. FIG. The experimental result of Example 7. FIG. The experimental result of Example 8. The experimental result of Example 9. FIG. The experimental result of Example 10. FIG. The experimental result of Example 11. The experimental result of Example 12. The experimental result of Example 13.

  The present invention is a method for producing an edible plant containing at least 10 mg / 100 g FW of at least one substance selected from the group consisting of vitamin U, taurine, carnosine, Tyr-Pro and L-carnitine. In the production method of the present invention, an edible plant is immersed in an aqueous solution containing the substance, and the substance is accumulated in the edible plant to produce an edible plant containing the substance. In the production method of the present invention, a part of the edible plant body is constantly or intermittently immersed in the aqueous solution, and the remainder is constantly or intermittently maintained outside the aqueous solution so that it can evaporate. Furthermore, edible plants and aqueous solutions are maintained at 20-35 ° C. Conventionally, for example, a method of adding a specific amino acid to an edible plant such as pickles is known. However, it is usually performed by immersing the entire edible plant body to which an amino acid is added in a solution containing a specific amino acid. On the other hand, in the production method of the present invention, a part of the edible plant body is constantly or intermittently immersed in an aqueous solution containing the substance, and the remainder is constantly or intermittently outside the aqueous solution so that it can evaporate. To maintain. Furthermore, in the method of adding a specific amino acid to edible plants such as pickles, the edible plant is maintained at a relatively low temperature (for example, around 5 ° C.) from the viewpoint of maintaining the freshness of the plant. In contrast, in the production method of the present invention, the temperature is maintained at 20 to 35 ° C. According to the experiments by the present inventors, in the production method of the present invention using transpiration, the transition of the substance to an edible plant is extremely slow at a temperature of less than 20 ° C., and the substance is added at 10 mg / 100 g FW or more. (However, for Tyr-Pro, 3 mg / 100 g FW or more) Containing edible plants could not be produced substantially.

  In the present invention, the edible plant can be, for example, a sprout. The sprout is also called a sprouting vegetable and is a germinated seed and a young plant growing from the seed. Depending on the type of plant, it is appropriate to use a plant of about 3 to 20 days after germination in the production method of the present invention. Plants that can become sprout are not particularly limited, but for example, cabbage, broccoli, mizuna, watercress, rucola, radish, common buckwheat, tartary buckwheat, green onion, leek, perilla, mint, basil, spinach, alfalfa, azuki bean Mitsuba, parsley, celery, wheat, barley, rice, etc. are suitable. In particular, Lucola, common buckwheat, tartary buckwheat, alfalfa, barley, radish, etc. are preferred.

  When a sprout is used in the production method of the present invention, the root of the sprout is constantly or intermittently immersed in the aqueous solution, and the part other than the root is constantly or intermittently maintained outside the aqueous solution. Specifically, it is preferable that a portion of 25% or more of the sprout is constantly or intermittently maintained outside the aqueous solution because a good transpiration effect is observed and the absorption of the material proceeds well.

  The sprout can be brought into contact with the aqueous solution in a rotating container, for example, a rotating drum used for manufacturing a curl sprout. In this case, the roots of the sprout are intermittently immersed in the aqueous solution, and other than the roots are intermittently maintained outside the aqueous solution. For example, the operating conditions of the rotating drum are suitably as follows. In other words, it is appropriate to rotate at a speed of about one revolution per minute that can be sufficiently exposed to the outside air under continuous illumination without damaging the plant body.

  Moreover, it can also be made to contact with the said aqueous solution in the container which left the sprout still. In that case, the roots of the sprout are always immersed in the aqueous solution, and other than the roots are always kept outside the aqueous solution.

  The edible plant can also be a cut vegetable. Examples of cut vegetables include, for example, cabbage, komatsuna, chingensai, Japanese cabbage, broccoli, watermelon, watercress, rucola, wasabi, aloe, asparagus, garlic, green onion, leek, green grass, wiping, mitsuba, parsley, celery, Wheat, barley, rice, etc. are suitable. In particular, broccoli, asparagus, green onions, leek, barley, barley and the like are preferable. When the edible plant body is a cut vegetable, the cut end surface of the vegetable stalk is constantly or intermittently immersed in the aqueous solution, and the remainder is constantly or intermittently maintained outside the aqueous solution. It is preferable that the vegetable solution is brought into contact with the aqueous solution in a container, the cut end face of the vegetable stalk is always immersed in the aqueous solution, and the remaining portion is always maintained outside the aqueous solution.

  Plants and aqueous solutions are maintained at a temperature of 20 ° C to 35 ° C. Preferably it is 25 to 30 ° C. When the temperature is below 20 ° C, sufficient absorption is not performed. Moreover, when it exceeds 35 degreeC, since not only sufficient absorption amount is not obtained but browning, wilt etc. arise, it is unpreferable. Moreover, it is preferable to perform immersion in aqueous solution under light irradiation rather than under darkness from a viewpoint of promoting a transpiration | evaporation effect and promoting substance absorption. By controlling the light conditions, the absorbability of the plant solution is further improved.

  The immersion of the edible plant body in the aqueous solution is suitably 6 hours or more and 24 hours or less. The contact is preferably performed for 6 to 16 hours. In this case, if the contact time is less than 6 hours, sufficient absorption may not be performed, and if it exceeds 24 hours, the treatment efficiency is not only poor, but the amount of absorption is the same and the plant body is contact treated. Etc. may be damaged. Rough contact time is 12 to 16 hours. The soaking time is appropriately set in consideration of the set temperature and the desired content of the substance in the edible plant body.

  The concentration of each substance in the GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine-containing aqueous solution is 0.1 to 10% concentration (weight concentration), 0.5% or more, preferably 1% The above is appropriate from the viewpoint of obtaining the desired content of the substance in a relatively short time. The upper limit of the concentration of each substance in the aqueous solution is preferably 5%. Under such conditions, an edible plant containing a high concentration of GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine is obtained.

  There is no particular limitation on the time when GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine is absorbed into the plant body, but GABA, vitamin U, taurine, carnosine, Tyr when the cultivation time after absorption treatment becomes longer. Since the accumulated content of Pro and / or L-carnitine tends to decrease, it is preferable to carry out the treatment at the final stage of cultivation. An example of the detailed absorption method to a plant body is demonstrated below. First, a GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine solution having a concentration of 0.1 to 10% is prepared. Specifically, GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine may be dissolved in normal water so as to have the above concentration. For the purpose of improving absorption efficiency, the solution contains GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine, liquid fertilizer such as mineral active liquid, chitosan and other fungicides, ascorbic acid and the like You may add antioxidant, the inhibitor of the enzyme which accelerates | stimulates browning of a structure | tissue, etc. For example, adding chitosan, catechin, ascorbic acid, citric acid, succinic acid, tartaric acid, ethyl alcohol, hinokitiol, minerals, calcium salts, etc. to the solution increases the absorption efficiency more stably and increases GABA, vitamin U, taurine, carnosine , Tyr-Pro and / or L-carnitine can be absorbed.

  In addition to the above GABA, vitamin U, taurine, carnosine, Tyr-Pro and L-carnitine, amino acids that are expected to be functional and peptides include amino acids that are essential amino acids, amino acid derivatives, peptides that are dipeptides, and tripeptides It can also be absorbed at the same time. As specific examples of these, essential amino acids include arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. Dipeptides and tripeptides include alanyl lysine, valinyl tyrosine, tyrosinyl proline. , Anserine, GSH and the like. Of the amino acids and peptides shown above, amino acids and peptides having high water solubility are particularly suitable for the present invention.

  Specifically, in sprouts such as so-called radish, rucola, tartoba, etc., the root portion at the bottom may be immersed in the solution for a certain period of time. Moreover, in vegetables, such as leeks, leek, honey bee, asparagus, and buckwheat, a stem including an edible portion may be cut from the root and the cut portion may be immersed in the solution. In these cases, the sprout / vegetable absorbs GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine through the tract using the water potential difference of transpiration. Therefore, in order to efficiently evaporate, it is important to expose organs other than the roots or cut surfaces to the air. As described above, by immersing plants in various forms in the treatment liquid as described above, a plant containing GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine at a high concentration can be simplified. It can be manufactured efficiently.

  According to the present invention, an edible plant having a GABA content of 10 mg / 100 gFW or more, preferably 55 mg / 100 gFW or more is obtained. About the upper limit of GABA content, although the upper limit amount which can be contained substantially changes with the kind of edible plant body, it is about 150 mg / 100gFW normally. For example, in the case of tartary buckwheat, GABA can be contained as long as it is about 100 mg / 100 g FW.

  According to the present invention, an edible plant having a vitamin U content of 10 mg / 100 gFW or more, preferably 70 mg / 100 gFW or more is obtained. About the upper limit of vitamin U content, although the upper limit amount which can be contained substantially changes with the kind of edible plant body, it is about 300 mg / 100gFW normally. For example, in the case of tartary buckwheat, if it is about 250 mg / 100 g FW, vitamin U can be contained.

  According to this invention, the edible plant body whose taurine content is 20 mg / 100gFW or more, Preferably it is 60 mg / 100gFW or more is obtained. About the upper limit of taurine content, although the upper limit amount which can be made to contain substantially changes with kinds of edible plant body, it is about 200 mg / 100gFW normally. For example, in the case of tartary buckwheat, if it is about 150 mg / 100 g, taurine can be contained.

  According to the present invention, an edible plant having a carnosine content of 15 mg / 100 gFW or more, preferably 50 mg / 100 gFW or more is obtained. About the upper limit of carnosine content, although the upper limit amount which can be made to contain substantially changes with kinds of edible plant body, it is about 120 mg / 100gFW normally. For example, in the case of tartary buckwheat, if it is about 92 mg / 100 g, carnosine can be contained.

  According to the present invention, an edible plant having a Tyr-Pro content of 3 mg / 100 gFW or more, preferably 10 mg / 100 gFW or more is obtained. About the upper limit of Tyr-Pro content, although the upper limit amount which can be contained substantially changes with kinds of edible plant body, it is about 40 mg / 100gFW normally. For example, in the case of tartary buckwheat, if it is about 29 mg / 100 g, Tyr-Pro can be contained.

  According to this invention, the edible plant body whose L-carnitine content is 15 mg / 100gFW or more, Preferably it is 50 mg / 100gFW or more is obtained. About the upper limit of L-carnitine content, although the upper limit amount which can be substantially contained changes with kinds of edible plant body, it is about 120 mg / 100gFW normally. For example, in the case of tartary buckwheat, L-carnitine can be contained in an amount of about 80 mg / 100 g.

  By eating a certain amount of the plant of the present invention, GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine which cannot normally be ingested naturally from the plant can be easily obtained. Ingestion becomes possible. In general, the content of various functional amino acids and peptides in a plant body varies depending on, for example, the cultivation method, cultivation conditions, individual differences, etc., even if the same type of plant is used. GABA, vitamin U, taurine Carnosine, Tyr-Pro and / or L-carnitine may be rarely contained. According to the present invention, the plant body is treated with GABA, vitamin U, taurine, carnosine, Tyr-Pro and / or L-carnitine solution, regardless of their differences, so that its accumulated content in the plant body becomes natural. On the other hand, it is possible to increase dramatically.

  Next, the present invention will be described in detail based on examples, but the following examples show preferred examples of the present invention, and the present invention is not limited to the examples.

Example 1
A vertical sprout of each plant was produced, and GABA, vitamin U, and taurine were absorbed into the sprout immediately before harvesting under the following conditions to produce a GABA, vitamin U, and taurine-rich sprout. The vertical sprout was carried out in accordance with a normal vertical sprout manufacturing method. That is, an appropriate amount of seeds was sown on a urethane mat, and irrigated for 20 minutes every 2 hours under 25 ° C. and light conditions. The absorption treatment was performed by the following method. Prepare 1% GABA, Vitamin U, Taurine solution and immerse the root parts of various sprout obtained by the above method in the above solution at 25 ° C for 16 hours under light conditions to absorb GABA, Vitamin U, Taurine I let you. The sprout of the comparative example was treated under the same conditions using water instead of the solution. Sprout GABA, vitamin U, and taurine were quantified by the method of Yamauchi et al. (Food Sci. Technol. Res., 10, 247-253, 2004) using high-performance liquid chromatography using the sprout immediately after production. The content is shown as the content per FW weight. The results are shown in FIG. 1 (GABA), FIG. 2 (vitamin U), and FIG. 3 (taurine). Thus, compared to the various sprouts of the comparative examples, the sprouts of the examples have sufficient amounts of GABA (each 20 mg / 100 gFW or more), vitamin U (each 15 mg / 100 gFW or more), taurine (50 mg / 100 gFW each). It can be seen that, according to the present invention, GABA, vitamin U and taurine-rich sprout can be produced easily and efficiently.

Example 2
For tartary buckwheat and barley sprout prepared in the same manner as in Example 1, GABA, vitamin U and taurine were simultaneously absorbed under the following conditions to produce a GABA, vitamin U and taurine-rich sprout. A solution containing 1% GABA, vitamin U and taurine was prepared, and the root part of the sprout was immersed in the solution at 25 ° C for 16 hours under light conditions to simultaneously absorb GABA, vitamin U and taurine. It was. The sprout of the comparative example was treated under the same conditions using water instead of the solution. Sprout GABA, vitamin U, and taurine were quantified using high performance liquid chromatography under the same conditions as in Example 1 using the sprout immediately after production. The content is shown as the content per FW weight. The results are shown in FIG. Thus, compared to the various sprouts of the comparative examples, the sprouts of the examples had sufficient amounts of GABA (each 50 mg / 100 gFW or more), vitamin U (each 50 mg / 100 gFW or more), taurine (80 mg / 100 gFW each). According to the present invention, it is possible to easily and efficiently absorb three types of functional substances simultaneously in the sprout. As a result, GABA, vitamin U, and taurine can be absorbed at high concentrations. It can be seen that the contained sprout can be produced.

Example 3
The curl sprout was performed according to a normal curl sprout manufacturing method. That is, an appropriate amount of seeds was sown in a rotating drum, and continuous irrigation was performed while rotating the drum under a light condition at 25 ° C. The absorption treatment was performed by the following method. Using the broccoli curl sprout produced by the above method, GABA, vitamin U, or taurine was absorbed under the following conditions immediately before harvesting to produce a GABA, vitamin U, taurine-rich curl sprout. A solution containing 0.5% GABA, 0.5% vitamin U and 0.5% taurine was prepared, and the entire sprout was brought into contact with the solution for 16 hours while rotating the drum at 25 ° C. and light conditions. The curl sprout of the comparative example was treated under the same conditions using water instead of the solution. The quantification of curl sprouts GABA, vitamin U and taurine was measured using high performance liquid chromatography under the same conditions as in Example 1 using the sprout immediately after production. The content is shown as the content per FW weight. The results are shown in FIG. Thus, compared with the various curl sprouts of the comparative examples, the curl sprouts of the examples have sufficient amounts of GABA (each 50 mg / 100 g FW or more), vitamin U (each 150 mg / 100 g FW or more), taurine (each 60 mg / 100 g FW or more), and according to the present invention, it is possible to easily and efficiently absorb a functional substance even in a specially shaped sprout such as a curl sprout. As a result, GABA, It can be seen that curl sprouts containing high concentrations of vitamin U or taurine can be produced.

Example 4
Commercially available mung bean sprouts were purchased and GABA, vitamin U and taurine were absorbed under the following conditions to produce mung bean sprouts with high GABA, vitamin U and taurine content. A solution containing 1% of each amino acid was prepared, and mung bean sprout roots were immersed in the solution at 20 ° C., 25 ° C., and 30 ° C. for 16 hours to absorb the amino acids. The sprout of the comparative example was treated under the same conditions using water instead of the solution. The quantitative determination of GABA, vitamin U, and taurine of mung bean sprouts was performed using high performance liquid chromatography under the same conditions as in Example 1 using mung bean sprouts immediately after production. The content is shown as the content per FW weight. The results are shown in FIG. From this, compared with each mung bean sprout of the comparative example, in the bean sprout of the example, a sufficient amount of amino acids absorbed in any of the test examples [GABA (each 50 mg / 100 g FW or more), vitamin U (each 80 mg / 100 g FW or more) ), Taurine (60 mg / 100 g FW or more each)] (however, at 30 ° C., the accumulated content of vitamin U tended to be relatively low), and according to the present invention, conventional methods such as mung bean sprouts It is understood that even bean sprouts can easily and efficiently absorb functional substances, and as a result, bean sprouts containing GABA, vitamin U, and taurine at high concentrations can be produced.

Example 5
In order to examine the stability of GABA, vitamin U, and taurine accumulation content during storage of GABA, vitamin U, and taurine accumulated plants, GABA, vitamin U, and taurine prepared in the same manner as in Example 1 were accumulated. The stability of GABA, vitamin U, and taurine content (storage test) was evaluated using the tartonic soba sprout that was used at 5 ° C., 95% humidity, and dark conditions. Sprout GABA, vitamin U, and taurine were measured using high performance liquid chromatography under the same conditions as in Example 1 after completion of the storage test. The content is shown as the content per FW weight. The result is shown in FIG. From these results, it was found that any sprout contained little GABA, vitamin U and taurine concentrations during storage and stably contained GABA, vitamin U and taurine, respectively, for a long period of time. From this, it can be seen that GABA, vitamin U, and taurine in a sprout with a high content of GABA, vitamin U, and taurine prepared by the method of the present invention are held very stably without being decomposed during storage.

Example 6
In order to observe the effect of GABA, vitamin U and taurine concentrations when absorbing GABA, vitamin U and taurine, GABA, vitamin U and taurine were used under the same conditions as in Example 1, using solutions with different concentrations. A tartrin-rich tartrin soup with vitamin U and taurine was prepared, and the effect of concentration on the absorption efficiency was evaluated. Sprout GABA, vitamin U, and taurine were quantified using high performance liquid chromatography under the same conditions as in Example 1 using the sprout immediately after production. The content is shown as the content per FW weight. The result is shown in FIG. From this, it was found that in the case of any solution of GABA, vitamin U, and taurine, a 0.5% or more solution was efficiently absorbed.

Example 7
In order to see the effect of soaking time when GABA, vitamin U and taurine are absorbed, the soaking time of GABA, vitamin U and taurine is changed, and under the same conditions as in Example 1, GATA, vitamin U and taurine-rich tartane soba sprout And the influence of the immersion time on the absorption efficiency and the like was evaluated. Sprout GABA, vitamin U, and taurine were quantified using high performance liquid chromatography under the same conditions as in Example 1 using the sprout immediately after production. The content is shown as the content per FW weight. The results are shown in FIG. From this, it has been clarified that the GABA, vitamin U, and taurine solutions can be efficiently absorbed when immersed for 16 hours or longer. However, in the case of GABA and taurine, it can be seen that even if the immersion time is extended to 24 hours, it is not much different from the case of 16 hours.

Example 8
Nagane, leek, honey bee, asparagus, and plantain are produced, and GABA, vitamin U, taurine is absorbed from the cut surface of the bottom of the vegetable under the same conditions as in Example 1 using 0.1% and 1% treatment solutions. Vitamin U and taurine-rich vegetables were prepared. The comparative vegetables were treated under the same conditions using water instead of the solution. GABA, vitamin U, and taurine in vegetables were quantified using high performance liquid chromatography under the same conditions as in Example 1 using the vegetables immediately after the treatment. The content is shown as the content per FW weight. The results are shown in FIG. Thus, GABA, vitamin U, and taurine are efficiently absorbed by the method of the present invention even in the case of normal vegetables as in the case of sprout and bean sprouts, and a GABA, vitamin U, and taurine-rich vegetable can be easily prepared. I understand.

Example 9
In order to see the effect of temperature and light conditions when GABA, vitamin U and taurine are absorbed, the GABA, vitamin U and taurine absorption temperature and light conditions are changed and GABA and vitamins are used under the same conditions as in Example 1. U and taurine-rich tartane soba sprout were prepared, and the effects of temperature and light on the absorption efficiency were evaluated. Sprout GABA, vitamin U, and taurine were quantified using high performance liquid chromatography under the same conditions as in Example 1 using the sprout immediately after production. The content is shown as the content per FW weight. The result is shown in FIG. From this, it was revealed that in the case of any solution of GABA, vitamin U, and taurine, when the immersion treatment is performed at 20 ° C. or higher, the solution is efficiently absorbed. In particular, at 20 ° C. and 25 ° C., it can be seen that the absorption efficiency under light conditions is good except for GABA at 25 ° C.

Example 10
Vertical sprout of each plant was produced, and carnosine, Tyr-Pro, and L-carnitine were absorbed into the sprout just before harvesting under the following conditions to produce carnosine, Tyr-Pro, and L-carnitine-rich sprout. The vertical sprout was carried out in accordance with a normal vertical sprout manufacturing method. That is, an appropriate amount of seeds was sown on a urethane mat, and irrigated for 20 minutes every 2 hours under 25 ° C. and light conditions. The absorption treatment was performed by the following method. A 1% carnosine, Tyr-Pro, L-carnitine solution was prepared, and the root parts of various sprout obtained by the above method were immersed in the above solution at 25 ° C. for 16 hours under light conditions. Carnosine, Tyr-Pro L-carnitine was absorbed. Sprout carnosine, Tyr-Pro, and L-carnitine were quantified by LC / MS using a sprout immediately after production. The results are shown in FIGS. 12 (1) (carnosine), (2) (Tyr-Pro), and (3) (L-carnitine). Thus, any sprout contains a sufficient amount of carnosine (each 82mg / 100gFW or more), Tyr-Pro (each 16mg / 100gFW or more), L-carnitine (each 84mg / 100gFW or more). It can be seen that carnosine, Tyr-Pro and L-carnitine-rich sprout can be produced easily and efficiently.

Example 11
To see the effect of carnosine, Tyr-Pro, and L-carnitine concentrations when absorbing carnosine, Tyr-Pro, and L-carnitine, use solutions with different concentrations of carnosine, Tyr-Pro, and L-carnitine. A carnosine, Tyr-Pro, L-carnitine-rich tartane soba sprout was prepared under the same conditions as in Example 1, and the effect of concentration on absorption efficiency and the like was evaluated. Sprout carnosine, Tyr-Pro, and L-carnitine were quantified using the sprout immediately after production under the same conditions as in Example 1. The content was expressed as the content per DW weight. The results are shown in FIGS. 13 (1) (carnosine), (2) (Tyr-Pro), and (3) (L-carnitine). From this, it was found that in the case of any of carnosine, Tyr-Pro, and L-carnitine, it was efficiently absorbed when a solution of 1% or more was used.

Example 12
In order to observe the effect of immersion treatment time when carnosine, Tyr-Pro, and L-carnitine are absorbed, the immersion time of carnosine, Tyr-Pro, and L-carnitine was changed, and carnosine, Tyr- Pro, L-carnitine high-content tartane soba sprout was prepared, and the effect of soaking time on absorption efficiency was evaluated. Sprout carnosine, Tyr-Pro, and L-carnitine were quantified using the sprout immediately after production under the same conditions as in Example 1. The content was expressed as the content per DW weight. The results are shown in FIGS. 14 (1) (carnosine), (2) (Tyr-Pro), and (3) (L-carnitine). From these results, it was found that any of the solutions of carnosine, Tyr-Pro, and L-carnitine can be efficiently absorbed by immersion for 16 hours or more.

Example 13
Carnosine prepared by the same method as in Example 1 in order to examine the stability of carnosine, Tyr-Pro, and L-carnitine accumulated content during storage of plants in which carnosine, Tyr-Pro, and L-carnitine were accumulated, The stability of carnosine, Tyr-Pro, and L-carnitine content (storage test) was evaluated at 5 ° C., 95% humidity, and dark conditions using a tartary soba sprout with Tyr-Pro and L-carnitine accumulated. Sprout carnosine, Tyr-Pro, and L-carnitine were measured under the same conditions as in Example 1 after the storage test. The content was expressed as the content per DW weight. The results are shown in FIGS. 15 (1) (carnosine), (2) (Tyr-Pro), and (3) (L-carnitine). From this, it was found that all the sprout contained carnosine, Tyr-Pro, and L-carnitine stably in the long term with little decrease in carnosine, Tyr-Pro, and L-carnitine concentrations during storage. . Therefore, carnosine, Tyr-Pro, and L-carnitine in sprout with high content of carnosine, Tyr-Pro, and L-carnitine prepared by the method of the present invention are held very stably without being degraded during storage. It can be seen that

  The present invention is useful in the agriculture and food fields such as vegetable production.

Claims (16)

Vitamin U, immersed taurine, carnosine, sprouts in an aqueous solution containing at least one substance selected from the group consisting of Tyr-Pro and L- carnitine, the material was accumulated contained in Sprout, containing the substance The accumulated substance is a method for producing a sprout having excellent storage stability ,
The sprout immerses roots in the aqueous solution constantly or intermittently, and other than the roots are constantly or intermittently maintained outside the aqueous solution,
The sprout and aqueous solution are maintained at 20-35 ° C.,
A method for obtaining a sprout containing at least 10 mg / 100 g FW or more of the above substance. The method of claim 1 in a container which rotates sprouts is contacted with the aqueous solution. The method according to claim 2 , wherein the aqueous solution is brought into contact with the aqueous solution in a container in which a sprout is placed. The method according to any one of claims 1 to 3, immersing 6-24 hours to the aqueous solution of the sprouts. The method according to any one of claims 1 to 4, wherein the substance is at least one substance selected from the group consisting of vitamin U and taurine. The method according to claim 1, wherein the substance is at least one substance selected from the group consisting of carnosine, Tyr-Pro, and L-carnitine. A sprout having a vitamin U content of 10 mg / 100 g FW or more, which is produced by immersing the sprout in an aqueous solution containing vitamin U and accumulating and containing the vitamin U in the sprout. Sprout with excellent storage stability . The sprout according to claim 7 , wherein the content of vitamin U is 70 mg / 100 g FW or more. A sprout having a taurine content of 20 mg / 100 g FW or more , manufactured by a method in which the sprout is immersed in an aqueous solution containing taurine, and the taurine is accumulated in the sprout, and the storage stability of the accumulated taurine is improved. Excellent sprout . The sprout according to claim 9 , wherein the taurine content is 60 mg / 100 g FW or more. A sprout having a carnosine content of 15 mg / 100 g FW or more , manufactured by a method of immersing the sprout in an aqueous solution containing carnosine and accumulating the carnosine in the sprout, and improving the storage stability of the accumulated carnosine Excellent sprout . The sprout according to claim 11 , wherein the carnosine content is 50 mg / 100 g FW or more. A sprout having a Tyr-Pro content of 3 mg / 100 g FW or more, which is manufactured and accumulated by immersing the sprout in an aqueous solution containing Tyr-Pro, and accumulating the Tyr-Pro in the sprout. A sprout with excellent storage stability of Tyr-Pro . The sprout according to claim 13 , wherein the Tyr-Pro content is 10 mg / 100 g FW or more. A sprout having an L-carnitine content of 15 mg / 100 g FW or more, which is produced and accumulated by immersing the sprout in an aqueous solution containing L-carnitine and accumulating the L-carnitine in the sprout. A sprout with excellent storage stability of L-carnitine . The sprout according to claim 15 , wherein the L-carnitine content is 50 mg / 100 g FW or more.