JP4848315B2 - Fatigue food and its manufacturing method - Google Patents

Description

  The present invention relates to a food, and more particularly, to a food suitable for preventing or improving fatigue eyes. The present invention also relates to a method for producing the food.

  The modern society is an era in which people continue to use their eyes such as driving a car, VDT (Visual Display Terminal) work, personal computers and televisions. In addition, the lifestyle itself imposes a burden on the eyes, such as an extremely short sleeping time and a long waking time. In terms of the environment, it can be said that the expansion of the ozone hole due to air pollution has dramatically increased the amount of ultraviolet rays reaching the ground, and the damage to the eyes due to ultraviolet rays has also increased. In this way, the modern society can be said to be an extremely burdensome environment for the eyes, and accordingly, the number of people who suffer from eye troubles such as tired eyes and eyestrain is increasing rapidly. Therefore, there is a demand for products that are expected to improve QOL (Quality Of Life), such as pharmaceuticals and foods that are effective in improving symptoms of eye problems such as fatigue.

  In general, tired eyes and eye strain are not recognized and used separately, but in the definition of specialized ophthalmology, the two are clearly distinguished. That is, fatigue eyes are physiological fatigue, and eyestrain falls into the category of pathological fatigue. In other words, it can be said that fatigued eyes are recovered by rest, and eyestrain does not improve the symptoms even by resting (Non-patent Document 1). However, depending on the degree of mental fatigue, eyestrain may become eyestrain, and eyestrain may just be eyestrain. Therefore, in reality, such a definition and distinction must be vague. . Some tired eyes recover by rest, but it takes time to recover, and the prognosis is so good that it is hard to say eye strain. There is no doubt that it is serious and morbid, but some have a very good prognosis and do not take so long to recover.

  Pathologically, the causes of eye strain are defined and roughly classified into three types: visual factors, internal environmental factors / mental factors, and external environmental factors (for example, Non-Patent Document 2). reference). In particular, it is speculated that external environmental factors occupy a very large weight, and light stimulating elements (harmful rays) directly damage the cornea, so ultraviolet rays are considered to be an important factor in eye strain. . The mechanism that causes damage to the cornea due to ultraviolet rays is still not completely understood (see, for example, Patent Document 1), but in particular, reactive oxygen species are caused by UV-B waves (Non-Patent Document 3). It is considered that damage is caused by reacting with lipids, proteins, DNA, etc. (see, for example, Non-Patent Document 4). On the other hand, the cause of tired eyes is not currently defined, and there is a fact that the cause is unknown. The main symptoms include eyes that are “shobo-shobo, flickering, throbbing, roaring, tingling, blurred”. In other words, the general term for eye troubles that exhibit such symptoms is classified as “fatigue eyes”, which is a kind of syndrome.

  Among such eye troubles, in particular, it has been necessary to deal with these problems in recent years because, as described above, the eyes are tired and require a long time to recover. This is because such symptoms have increased rapidly in recent years and the underlying cause is unknown. For this reason, it can be said that there is only a palliative response to such fatigued eyes by sufficiently resting the eyes and administering eye drops containing vitamins and the like. In view of such a situation, the present inventors have found that an extract of a honeysuckle family honeysuckle genus lotus cup has such a fatigue eye prevention and improvement effect (for example, Patent Document 1). However, when further examination was made, it was found that there were many variations in the effect depending on the collection time of the Hascup and the production area. That is, it has been desired to develop a technique for stably maintaining the effect of this useful material for improving fatigue eyes.

  On the other hand, an anthocyanin in blueberries is known as a component that exhibits a favorable action on fatigue eyes (see, for example, Patent Document 2), but the amount of anthocyanin contained in a lotus cup is as large as its effect. The relationship between the variation in effect and the amount of anthocyanin was also unclear.

  On the other hand, it is already known that berries contain cyanidin glycosides in addition to anthocyanins (see, for example, Patent Document 3). Moreover, it is already known that such cyanidin glycosides have an anti-obesity action, an anti-diabetic action, and an anti-tumor action, and it has been reported that this is due to a very strong antioxidant activity (for example, (See Patent Document 4 and Patent Document 5). It is also known that cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside stimulate rhodopsin resynthesis (see, for example, Non-Patent Document 5). However, it is not known at all that this component is an active body for the improvement and prevention of fatigue eye of the Hascup extract, and by controlling the amount of such component, the effect of improving and preventing the fatigue eye of the Hascup extract It is not known at all that it is possible to suppress the variation of.

JP 2007-089450 A JP 2001-224320 A WO2002 / 022847 JP 2003-252766 A JP 2000-178295 A Kazunari Atsumi, “Adjustment / eye strain”, 5-6, Kodansha, 2001 Inoue Masayasu et al., "Fatigue Science", pp. 17-26, Kanehara Publishing, 1999 Zigman.S Photochem Photobiol. 57 (6), 1060-1068 (1993) J.CEJKOVA et al. Physiol.Res. 53, 1-10 (2004) Matsumoto H. et.al., J Agric Food Chem. 2003; 51 (12): 3560-3.

  The present invention has been made under such circumstances, and an object of the present invention is to provide a technique for stably maintaining the effect of a useful fatigue eye improving material.

In view of such a situation, the present inventors have sought for a technique for stably maintaining the effect of a useful fatigue eye improving material, and as a result of intensive research efforts, as a result, cyanidin-3-O-alcohol has been obtained. By designing the food containing the fatigue eye ameliorating material so that the sugar is an active ingredient having such an action and the daily intake of the ingredient is 1-1000 mg, I found that it could be maintained and completed the invention. That is, the present invention is as follows.
(1) A food for improving or preventing fatigue eyes containing cyanidin-3-O-glycoside as an active ingredient, wherein the daily intake of the cyanidin-3-O-glycoside is 1 A food product characterized by being -1000 mg.
(2) The food according to (1), wherein the cyanidin-3-O-glycoside contains both cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside.
(3) The food according to (2), wherein the content of cyanidin-3-O-rutinoside is 1 to 10% by mass of the content of cyanidin-3-O-glucoside.
(4) The base source of the cyanidin-3-O-glycoside is an extract of a fruit of a lotus cup (Lonicera caerulea L.) belonging to the genus Honeysuckle family Lonicera caerulea L. (1) to (3 food of any crab described).
(5) The food according to (4) , characterized in that the lotus cup (Lonicera caerulea L.) belonging to the genus Honeysuckle family is from the People's Republic of China.
(6) A food for preventing or ameliorating fatigue eyes, which contains an extract of a lotus fruit (Lonicera caerulea L.) fruit that belongs to the honeysuckle family Lonicera caerulea L. .) Food extract, wherein the content of cyanidin-3-O-glycoside in the fruit extract is 3 to 30% by mass.
(7) The food according to (6), wherein the cyanidin-3-O-glycoside contains both cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside.
(8) Concentrating an extract of a lotus fruit (Lonicera caerulea L.) fruit belonging to the genus Honeysuckle family, and quantifying cyanidin-3-O-glucoside and cyanidin-3-O-lutinoside in the concentrate. In addition, it is confirmed that the content of cyanidin-3-O-glycoside is 3 to 30% by mass with respect to the total amount of the concentrate, and this is blended to prevent fatigue eyes. Or the manufacturing method of the food for improvement.

  ADVANTAGE OF THE INVENTION According to this invention, the technique which maintains stably the effect of a useful fatigue eye improvement raw material can be provided.

  The food of the present invention is a food for improving or preventing fatigue eyes, containing cyanidin-3-O-glycoside as an active ingredient, and the cyanidin-3-O-glycoside per day The intake is 1-1000 mg. Usually, such foods for improving or preventing fatigue eyes are known to contain an extract of bilberries such as blueberries, which are fruits of a plant belonging to the genus Vaccinaceae. This is because the fruit of bilberries has a high content of anthocyanins, and it is considered that such anthocyanins exert a favorable action on the resynthesis of rhodopsin. However, in the study of the present inventor, as shown in the examples below, even when the anthocyanin content is similar to that of bilberries, in the fruit extract of the lotus cups The improvement and prevention effects of fatigued eyes over bilberries were observed. This means that the amount of anthocyanin alone cannot explain the effect of improving and preventing fatigue eyes. Based on this finding, the present inventors compared the components contained in the fruit extract of bilberry and lotus cup. In the present invention, the lotus cup is a generic term for the fruits of Lonicera caerulea L. ssp. Edulis Hulten or Lonicera caerulea L. ssp. Edulis Hulten var. Emphyllocalyx Nakai. The fruit of edulis Hulten is particularly preferred.

<Comparative test example>
In accordance with the procedure shown below, the components contained in the extract of bilberry and hascup were compared. The lotus cups from Hokkaido and Heilongjiang, China were used, and the bilberries from Finland were used. Crush 100 g of fruit, irradiate with ultrasonic waves for 30 minutes, centrifuge (2000 rpm, 20 minutes), take out the fruit juice, add 50 ml of 1% hydrochloric acid and 50% aqueous methanol solution to the residue, irradiate with ultrasonic waves for 30 minutes, Centrifugation (2000 rpm, 20 minutes), the supernatant was combined with fruit juice, 1% hydrochloric acid-added 50% methanol aqueous solution was added to make up to 100 ml, and the mixture was filtered through a 0.45 μm “Millipore” filter. Which using HPLC, anthocyanin total under the following conditions, cyanidin-3-O - glucoside (. Hereinafter, sometimes abbreviated as C3G) amount, cyanidin-3-O-Le Chi Noshido (hereinafter, the C3R The amount was quantified by the absolute calibration curve method. The results are shown in FIG. Hascup and bilberry were almost the same in terms of the total content of anthocyanins, but C3G and C3R were significantly more in Hascup. In addition, significant differences also in Hokkaido and China Heilongjiang production in Lonicera caerulea has exist. From the difference in effects shown below, it can be seen that, in fatigue, the effect of improving and preventing fatigue is regulated by the content of cyanidin glycosides such as C3G and C3R rather than the total amount of anthocyanins. In this sense, it can be seen that a lotus cup of anthocyanins with a high cyanidin glycoside content, especially a lotus cup made in China is preferable as a base of an active ingredient for improving and preventing fatigue eyes.
(HPLC conditions) Column: Develosil RPAQUEOUS (C-30, id 4.6 × 250 mm) (Nomura Chemical Co. Ltd), detector: PDA detector 520 nm, flow rate: 1 mL / min, column temperature: 40 ° C., mobile phase: 0.05 % Phosphoric acid, 8% CH ₃ CN: 0.05% phosphoric acid, 30% CH ₃ CN = 100: 0 → 0: 100 (linear gradient)

  Thus, it can be seen that the effect of improving and preventing fatigue is explained by the content of cyanidin glycoside. That is, the amount of cyanidin glycoside in the lotus cup or bilberry is quantified, and the preparation is adjusted so that the amount is constant, thereby providing a food with a stable effect at all times. As such an adjustment method, the cyanidin glycoside of the concentrate of the extract of the fruit of the cup as a raw material is measured, and the extract containing a large amount of cyanidin glycoside and the inclusion of the cyanidin glycoside in advance It can preferably be exemplified by preparing an extract with a small amount and mixing them appropriately in light of the measurement results to adjust the content of cyanidin glycoside.

The content of the cyanidin glycoside in the fruit extract of a lotus cup containing such a cyanidin glycoside is preferably adjusted so that the daily intake in the preparation is 1-1000 mg. More preferably, it can be adjusted to 2 to 500 mg, more preferably 3 to 100 mg. In order to achieve such conditions, a concentrate of a Hascup extract having a cyanidin-3-O-glycoside content of 3 to 30% by mass, more preferably 5 to 25% by mass, It can be exemplified to be contained in a preparation of ˜50 mass%. To appropriately once such preparations taken several times, it is preferable to ingest cyanidin glycosides of said range of amount. In formulating such a preparation, it is possible to contain any optional ingredients that are usually used in foods. Examples of such optional components include sugars such as lactose and reduced maltose, binders such as gum arabic and xanthan gum, disintegrants such as crystalline cellulose and starch, coating agents such as zein and shellac, flavoring agents, A dispersing agent, an emulsifier, a sugar coating agent, etc. can be illustrated suitably. By treating the essential component and the optional component according to a conventional method, the food of the present invention can be applied without particular limitation as long as it is a dosage form known as food, for example, tablets, powders, It can be processed into granules including powdered juice.

  Hereinafter, the present invention will be described in more detail with reference to examples, but it goes without saying that the present invention is not limited to such examples.

<Production Example 1>
And ulcers to fruit 100g of Lonicera caerulea China Heilongjiang production used in Comparative Test Examples, to which 50% aqueous ethanol 200ml was added, ultrasound was irradiated for 10 min, centrifuged (1000
rpm × 10 minutes) to obtain a supernatant. The supernatant was concentrated under reduced pressure and then freeze-dried to obtain 12.5 g of amorphous. This was analyzed under the above-mentioned HPLC conditions, and the content of cyanidin glycoside was determined. As a result, it contained 3.2% by mass of C3G and 0.1% by mass of C3R. The total amount of anthocyanins was 3.4%.

<Production Example 2>
The fruit of the lotus cup of Production Example 1 was replaced with the fruit of Finnish bilberry, and the same operation was performed to obtain 13.3 g of amorphous. When the content of the cyanidin glycoside was determined, it contained 0.3% by mass of C3G. The presence of C3R was not recognized. The total amount of anthocyanins was 3.1%.

<Production Example 3>
The food 1 (tablet) of the present invention was produced according to the following formulation. That is, the prescription components were charged into a pneumomerizer (manufactured by Fuji Powder Co., Ltd.), and while spraying 50 parts by mass of water, warm air at 40 ° C. was fed to perform flow rolling granulation. After granulation, warm air of 40 ° C. was further blown for 2 hours and dried, and the resulting granule was tableted to obtain a 200 mg tunable tablet.

<Production Example 4>
The amorphous product of Production Example 1 of Production Example 3 was replaced with the amorphous product of Production Example 2 and treated in the same manner to obtain a tunable tablet of Comparative Example 1.

<Drinking test>
About the foodstuff 1 of the manufacture example 3, and the comparative example 1 of the manufacture example 4, the drinking test by the panelist 30 persons (2 groups) of 15 eyes per group was done. The drinking period was 2 months, and 6 tablets were taken 3 times a day in the morning, noon and evening. The food 1 group will receive 59.4 mg of cyanidin glycoside per day, and the group of Comparative Example 1 will receive 5.4 mg of cyanidin glycoside. Before and after the test, eye thermography, flicker test, near point position, dark adaptation were measured, and changes due to drinking were observed. The results are shown in FIG. 2 (flicker test), FIG. 3 (near point position), and FIG. 4 (dark adaptation). From this, it can be seen that food 1 is significantly superior to Comparative Example 1 in improving and preventing fatigue eyes. That is, it can be seen that among the anthocyanins, cyanidin glycosides greatly contribute to the improvement and prevention of fatigue eyes. It can be seen that by controlling the amount of cyanidin glycoside, the improvement / prevention of fatigue eyes can be controlled.

The specific measurement procedure is shown below.
(1) Thermographic measuring instrument: Nippon Avionics Co., Ltd. Neo Thermo TVS-700
Measurement environment: room temperature 25 ± 2 ℃, humidity 50 ± 15%
Procedure: Acclimatization was performed for 10 minutes after entering a room of constant temperature and humidity, and thermography was taken.
(2) Flicker test measuring instrument: Shibata Science Co., Ltd. Gakken digital flicker value measuring instrument RDF-1
Measurement environment: Not specified (actually performed in the same room as the thermography)
Procedure: After acclimatization like thermography, flicker value measurement 5 times / set x 3 sets are measured (the first 1 set is actually dark adaptation and 2 to 3 sets are actually analyzed)
(3) Near-point position measuring instrument: Hamamatsu Photonics Co., Ltd. Kimimi reaction measuring device TriIRIS C9000
Measurement environment: Not specified (actually performed in the same room as the thermography)
Measurement procedure: After acclimatization similar to thermography, measured 5 times (4) Dark adaptation Measuring instrument: None Measurement environment: Room measurement under constant light intensity Procedure: Acclimatization for 20 minutes after entering the room, after turning off, about 30 cm away The time to recognize the Randle ring, which is a standard index for visual acuity screening, was measured.

  The present invention can be applied to foods such as health foods.

It is a figure which shows the content pattern of the anthocyanin for every fruit. It is a figure which shows the result of the drinking test of Example 1. It is a figure which shows the result of the drinking test of Example 1. It is a figure which shows the result of the drinking test of Example 1.

Claims (1)

Concentrated fruit extract of Caprifoliaceae honeysuckle belonging Lonicera caerulea (Lonicera caerulea L.), and cyanidin -3-O-glucoside in concentrated Chijimibutsu include cyanidin -3-O-rutinoside, cyanidin-3- The content of O-rutinoside is 1 to 10% by mass of the content of cyanidin-3-O-glucoside, and the content of cyanidin-3-O-glycoside with respect to the total concentrate is 3 to 30% by mass. The method for producing a food for preventing or ameliorating fatigue eyes , comprising adjusting and blending the composition so as to be %.

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