JP4797167B2 - Embedding cassette - Google Patents

Embedding cassette Download PDF

Info

Publication number
JP4797167B2
JP4797167B2 JP2006009806A JP2006009806A JP4797167B2 JP 4797167 B2 JP4797167 B2 JP 4797167B2 JP 2006009806 A JP2006009806 A JP 2006009806A JP 2006009806 A JP2006009806 A JP 2006009806A JP 4797167 B2 JP4797167 B2 JP 4797167B2
Authority
JP
Japan
Prior art keywords
cassette body
cassette
embedding
insole member
tissue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2006009806A
Other languages
Japanese (ja)
Other versions
JP2007192616A (en
Inventor
伸行 金子
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Tokyo NUC
Original Assignee
University of Tokyo NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Tokyo NUC filed Critical University of Tokyo NUC
Priority to JP2006009806A priority Critical patent/JP4797167B2/en
Publication of JP2007192616A publication Critical patent/JP2007192616A/en
Application granted granted Critical
Publication of JP4797167B2 publication Critical patent/JP4797167B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

本発明は、組織標本を作製するための包埋用カセットの改良に関する。   The present invention relates to an improvement of an embedding cassette for producing a tissue specimen.

従来より、病理組織学的検査には、生体の一部から切除した組織片から顕微鏡用の組織標本を作製して行われている。この組織標本は、上記組織片に対して脱水処理等の前処理を施し、パラフィン等で包埋した後薄切して作製される。組織片を包埋処理するまでの工程では、組織片を包埋用カセットに入れ、処理中の変形を抑制するために、スポンジ材質のバイオプシーシートにより組織片を押さえつつ各工程を行っていた。   Conventionally, histopathological examination has been performed by preparing a tissue specimen for a microscope from a tissue piece excised from a part of a living body. This tissue specimen is prepared by subjecting the tissue piece to pretreatment such as dehydration, embedding with paraffin, and then slicing it. In the process up to embedding the tissue piece, the tissue piece is put in an embedding cassette, and in order to suppress deformation during the process, each process is performed while pressing the tissue piece with a sponge biopsy sheet.

なお、組織標本の作製は、例えば下記特許文献１、特許文献２及び非特許文献１に開示されている。
特開２００３−２１５００４号公報 特開２００４−３７２１５号公報 病理学／病理組織細胞学 第４編 組織学的検査法 春日孟 松原修 医歯薬出版株式会社 The preparation of the tissue specimen is disclosed in, for example, the following Patent Document 1, Patent Document 2, and Non-Patent Document 1.
JP 2003-215004 A JP 2004-37215 A Pathology / Histopathology Cytology 4 Histological examinations Kaoru Kasuga Osamu Matsubara Medical and Dental Publishing Co., Ltd.

しかし、上記従来の技術においては、前処理工程で使用されるアセトン等の薬液がスポンジ材質のバイオプシーシートにより後工程に持ち越され、薬液、パラフィン等が汚染されるという問題があった。   However, the conventional technique has a problem that the chemical solution such as acetone used in the pretreatment process is carried over to the subsequent process by the biopsy sheet made of sponge material, and the chemical liquid, paraffin and the like are contaminated.

特に、近年では、上記組織標本を作製する工程の内、前処理及びパラフィン等で包埋する処理を自動で行う装置が普及しているが、薬液の持ち越し量が多くなると、装置中に貯蔵された薬液、パラフィン等の交換頻度が高くなり、組織標本の作製コストが高くなると共に、組織標本の作製不良を招く可能性があるという問題があった。   In particular, in recent years, devices that automatically perform pretreatment and embedding with paraffin or the like among the steps of preparing the tissue specimen have become widespread, but when the carry-over amount of the chemical solution increases, it is stored in the device. In addition, there is a problem that the frequency of exchanging chemical liquids, paraffin, and the like is increased, the cost of preparing the tissue specimen is increased, and there is a possibility that the preparation of the tissue specimen is defective.

本発明は、上記従来の課題に鑑みなされたものであり、その目的は、処理中の変形を抑制したまま、薬液の持ち越し量を低減でき、組織標本の作製コストを低減すると共に、組織標本の作製不良を回避できる包埋用カセットを提供することにある。   The present invention has been made in view of the above-described conventional problems, and its purpose is to reduce the carry-over amount of a chemical solution while suppressing deformation during processing, to reduce the preparation cost of a tissue specimen, An object of the present invention is to provide an embedding cassette that can avoid defective production.

上記目的を達成するために、本発明は、組織標本を作製するための包埋用カセットであって、底面に複数の貫通孔またはスリットが形成され、上面が開口した箱形のカセット本体と、少なくとも表面が液体の非吸収性を有するとともに、前記カセット本体の底面と略同一の形状であり、前記開口から前記カセット本体に落とし込み可能であって、貫通孔またはスリットが形成された板状の中敷部材と、を備えることを特徴とする。   In order to achieve the above object, the present invention is an embedding cassette for producing a tissue specimen, wherein a plurality of through holes or slits are formed on a bottom surface, and a box-shaped cassette body having an open top surface; At least the surface has a non-absorbing property of liquid and is substantially the same shape as the bottom surface of the cassette body, and can be dropped into the cassette body from the opening, and has a plate-like shape in which a through hole or a slit is formed. And a floor member.

上記構成によれば、中敷部材の少なくとも表面が液体の非吸収性を有するので、薬液の持ち越し量を低減することができる。   According to the above configuration, since at least the surface of the insole member has liquid non-absorbability, the carry-over amount of the chemical solution can be reduced.

また、上記中敷部材に形成された貫通孔またはスリットは、前記カセット本体の底面に形成された貫通孔またはスリットと略同一であってもよい。   The through hole or slit formed in the insole member may be substantially the same as the through hole or slit formed in the bottom surface of the cassette body.

また、上記カセット本体と前記中敷部材とは、樹脂製であるのが好適である。   The cassette body and the insole member are preferably made of resin.

以下、本発明を実施するための最良の形態（以下、実施形態という）を、図面に従って説明する。   Hereinafter, the best mode for carrying out the present invention (hereinafter referred to as an embodiment) will be described with reference to the drawings.

図１には、本発明にかかる包埋用カセットの構成例の斜視図が示される。図１において、包埋用カセットには、上面が開口した箱形のカセット本体１０と、板状の中敷部材１２とが含まれる。これらのカセット本体１０及び中敷部材１２は、少なくとも表面が液体の非吸収性を有し、例えば樹脂で形成することができる。この樹脂としては、例えばポリアセタール（商品名：ジュラコン）等を使用することができる。ここで、液体の非吸収性を有するとは、スポンジ材質のような多孔性の表面ではなく、水、有機溶媒等の液体を吸収しない性質を有することをいう。   FIG. 1 shows a perspective view of a configuration example of an embedding cassette according to the present invention. In FIG. 1, the embedding cassette includes a box-shaped cassette body 10 whose upper surface is open and a plate-like insole member 12. The cassette body 10 and the insole member 12 have a non-absorbing property at least on the surface, and can be formed of, for example, resin. As this resin, for example, polyacetal (trade name: Duracon) or the like can be used. Here, having liquid non-absorptivity means having a property of not absorbing a liquid such as water or an organic solvent rather than a porous surface such as a sponge material.

カセット本体１０の底面には、複数の貫通孔１４が形成されている。図１に示された例では、貫通孔１４の形状が矩形となっており、正方形、長方形の何れであってもよい。   A plurality of through holes 14 are formed in the bottom surface of the cassette body 10. In the example shown in FIG. 1, the shape of the through hole 14 is a rectangle, and may be either a square or a rectangle.

また、中敷部材１２は、カセット本体１０の底面と略同一で若干小さい形状とされ、カセット本体１０の開口からカセット本体１０の底面上に落とし込み可能に構成されている。この中敷部材１２には、複数の貫通孔１６が形成されている。なお、中敷部材１２に形成された貫通孔１６は、カセット本体１０の底面に形成された貫通孔１４と略同一の形状とされているが、他の形状とすることもできる。   The insole member 12 is substantially the same as the bottom surface of the cassette body 10 and has a slightly smaller shape. The insole member 12 can be dropped onto the bottom surface of the cassette body 10 from the opening of the cassette body 10. A plurality of through holes 16 are formed in the insole member 12. In addition, although the through hole 16 formed in the insole member 12 has substantially the same shape as the through hole 14 formed in the bottom surface of the cassette body 10, other shapes may be used.

図２には、カセット本体１０及び中敷部材１２の変形例の平面図が示される。図２においても、中敷部材１２は、カセット本体１０の底面と略同一で若干小さい形状とされ、カセット本体１０の開口からカセット本体１０の底面上に落とし込み可能に構成されている。また、カセット本体１０及び中敷部材１２には、円形の貫通孔１４及び貫通孔１６がそれぞれ形成されている。これらの貫通孔１４及び貫通孔１６は、互いに同じ半径としてもよいし、異なる半径としてもよい。また、円形に限らず、楕円形等とすることもできる。   FIG. 2 shows a plan view of a modified example of the cassette body 10 and the insole member 12. Also in FIG. 2, the insole member 12 is substantially the same as the bottom surface of the cassette body 10 and has a slightly smaller shape, and is configured to be dropped onto the bottom surface of the cassette body 10 from the opening of the cassette body 10. The cassette body 10 and the insole member 12 are each formed with a circular through hole 14 and a through hole 16. The through holes 14 and the through holes 16 may have the same radius or different radii. Further, the shape is not limited to a circle, and may be an ellipse or the like.

図３には、カセット本体１０及び中敷部材１２の他の変形例の平面図が示される。図３においても、中敷部材１２は、カセット本体１０の底面と略同一で若干小さい形状とされ、カセット本体１０の開口からカセット本体１０の底面上に落とし込み可能に構成されている。また、カセット本体１０及び中敷部材１２には、スリット状の貫通孔１４及び貫通孔１６がそれぞれ形成されている。これらの貫通孔１４及び貫通孔１６は、互いに同じ大きさのスリットとしてもよく、異なる大きさであってもよい。   FIG. 3 shows a plan view of another modified example of the cassette body 10 and the insole member 12. Also in FIG. 3, the insole member 12 is substantially the same as the bottom surface of the cassette body 10 and has a slightly smaller shape, and is configured to be dropped onto the bottom surface of the cassette body 10 from the opening of the cassette body 10. The cassette body 10 and the insole member 12 are formed with slit-like through holes 14 and 16, respectively. The through hole 14 and the through hole 16 may be slits having the same size or different sizes.

図４には、図１、図２及び図３に示された包埋用カセットを使用した包埋工程の説明図が示される。図４において、包埋用カセットのカセット本体１０には、生体の一部から切除した組織片１８が収容され（Ｓ１）、板状の中敷部材１２がカセット本体１０の開口から落とし込まれ、組織片１８の上に載せられて、包埋処理中の組織片１８の変形を防止する（Ｓ２）。   FIG. 4 shows an explanatory view of an embedding process using the embedding cassette shown in FIGS. In FIG. 4, the cassette body 10 of the embedding cassette contains a tissue piece 18 excised from a part of the living body (S1), and the plate-like insole member 12 is dropped from the opening of the cassette body 10. The tissue piece 18 placed on the tissue piece 18 is prevented from being deformed during the embedding process (S2).

次に、上記組織片１８が収容されたカセット本体１０を、パラフィン処理装置２０の内部に載置し、アセトン等の脱水剤を使用して組織片１８の脱水処理を行う（Ｓ３）。この場合、脱水剤はカセット本体１０の底面及び中敷部材１２に形成された貫通孔１４、１６を介して流動し、組織片１８に接触する。   Next, the cassette body 10 containing the tissue piece 18 is placed inside the paraffin processing apparatus 20, and the tissue piece 18 is dehydrated using a dehydrating agent such as acetone (S3). In this case, the dehydrating agent flows through the bottom surface of the cassette body 10 and the through holes 14 and 16 formed in the insole member 12 and contacts the tissue piece 18.

上記脱水処理の終了後、装置内の脱水剤を排液する。この場合にも、カセット本体１０の底面及び中敷部材１２に貫通孔１４、１６が形成されているので、カセット本体１０内から脱水剤を速やかに排液することができる。続いて、キシロール等の中間処理剤を使用して組織片１８から上記脱水剤を除去し、組織片１８と後に使用するパラフィンとの親和性を向上させる処理を行う（Ｓ４）。この中間処理剤も、カセット本体１０の底面及び中敷部材１２に形成された貫通孔１４、１６を介して流動し、組織片１８に接触する。親和性向上処理の終了後、装置内の中間処理剤を排液する。   After the dehydration process is completed, the dehydrating agent in the apparatus is drained. Also in this case, since the through holes 14 and 16 are formed in the bottom surface of the cassette body 10 and the insole member 12, the dehydrating agent can be quickly drained from the cassette body 10. Subsequently, an intermediate treatment agent such as xylol is used to remove the dehydrating agent from the tissue piece 18, and a process for improving the affinity between the tissue piece 18 and paraffin to be used later is performed (S4). The intermediate treatment agent also flows through the through holes 14 and 16 formed in the bottom surface of the cassette body 10 and the insole member 12 and comes into contact with the tissue piece 18. After the affinity improving process is completed, the intermediate processing agent in the apparatus is drained.

次に、パラフィン処理装置２０内にパラフィンを供給し、組織片１８にパラフィンを浸透させる処理を行う（Ｓ５）。パラフィンは、上記同様、カセット本体１０の底面及び中敷部材１２に形成された貫通孔１４、１６を介して流動し、組織片１８に接触する。   Next, the paraffin is supplied into the paraffin processing apparatus 20, and the tissue piece 18 is infiltrated with paraffin (S5). As described above, the paraffin flows through the through holes 14 and 16 formed in the bottom surface of the cassette body 10 and the insole member 12 and contacts the tissue piece 18.

その後、パラフィン処理装置２０からカセット本体１０を取り出し、パラフィン浸透処理後の組織片１８をパラフィン包埋処理プレート２２に移す。このパラフィン包埋処理プレート２２には、組織片１８を収容する凹所２４が形成されており、この凹所２４に組織片１８を収容した後、カセット本体１０により蓋をする。この状態でパラフィン包埋処理プレート２２にパラフィンを追加し、パラフィン包埋処理を行う（Ｓ６）。   Thereafter, the cassette body 10 is taken out from the paraffin processing apparatus 20, and the tissue piece 18 after the paraffin infiltration processing is transferred to the paraffin embedding processing plate 22. The paraffin embedding plate 22 is formed with a recess 24 for receiving the tissue piece 18, and after the tissue piece 18 is received in the recess 24, the cassette body 10 is capped. In this state, paraffin is added to the paraffin embedding processing plate 22 and paraffin embedding processing is performed (S6).

以上により、パラフィンに包埋された組織片１８をカセット本体１０の底面上で作製することができる。   As described above, the tissue piece 18 embedded in paraffin can be produced on the bottom surface of the cassette body 10.

上記包埋処理において、本実施形態にかかるカセット本体１０及び中敷部材１２を使用すると、処理中の変形を抑制したまま、脱水剤、中間処理剤等が次工程に持ち越される量を低減することができ、使用薬液の劣化を抑制して薬液の交換頻度を低減することができる。これにより、組織標本の作製不良を回避することができる。   In the embedding process, when the cassette body 10 and the insole member 12 according to the present embodiment are used, the amount of dehydrating agent, intermediate treatment agent, etc. carried forward to the next process is reduced while suppressing deformation during the process. It is possible to suppress the deterioration of the chemical solution used and reduce the frequency of replacement of the chemical solution. Thereby, the production defect of a tissue specimen can be avoided.

また、上記中敷部材１２は、検体組織を削ぐツールとしても使用することができる。すなわち、軟らかい検体組織をカセット本体１０に入るように削ぐ（トリミング）ことはフリーハンドでは困難である。そのような場合は、カセット本体１０に検体組織を入れ、カセット本体１０の開口部に沿ってメスなどでトリミングを行うことにより容易に検体組織を削いで、カセット本体１０に入れることができる組織片１８を得ることができる。   The insole member 12 can also be used as a tool for scraping the specimen tissue. In other words, it is difficult to trim a soft specimen tissue so as to enter the cassette body 10 by freehand. In such a case, a tissue piece is placed in the cassette body 10 by placing the specimen tissue in the cassette body 10 and trimming the specimen tissue along the opening of the cassette body 10 with a scalpel or the like. 18 can be obtained.

このような方法でトリミングを行うと、検体組織がカセット本体１０と同じ厚さになるため、蓋を閉めたときに検体組織が押しつぶされ、薬液が通過するカセット本体１０の貫通孔１４が検体組織でふさがってしまう。そのため、カセット本体１０内に薬液が浸透せず、薬液交換不良が起こり不良標本になることがある。この問題を解決するため、中敷部材１２をカセット本体１０内に１枚あるいは複数枚落とし入れてから検体組織を入れトリミングを行えば、トリミングの容易さは維持したまま任意の厚さに検体を薄く削ぐ事ができ、また、落とし入れた中敷部材１２の枚数を減らして蓋を閉めることで、貫通孔１４をふさぐことも防止でき、さらにはカセット本体１０内に若干の空間を保つことが出来るため、薬液浸透不足による標本の作成不良を防ぐことが出来る。   When trimming is performed by such a method, the sample tissue becomes the same thickness as the cassette body 10, so that the sample tissue is crushed when the lid is closed, and the through-hole 14 of the cassette body 10 through which the chemical solution passes is formed in the sample tissue. It will be blocked. For this reason, the chemical liquid does not penetrate into the cassette body 10 and chemical liquid exchange failure may occur, resulting in a defective specimen. In order to solve this problem, if one or a plurality of insole members 12 are dropped into the cassette body 10 and then the specimen tissue is inserted and trimmed, the specimen can be adjusted to an arbitrary thickness while maintaining the ease of trimming. It can be thinned, and by closing the lid by reducing the number of insole members 12 dropped, it is possible to prevent the through-hole 14 from being blocked, and to maintain some space in the cassette body 10. Therefore, it is possible to prevent a sample preparation failure due to insufficient chemical penetration.

以下、本発明の効果確認試験を実施例として説明する。   Hereinafter, the effect confirmation test of the present invention will be described as examples.

底面に、直径１ｍｍの貫通孔１４が形成されたカセット本体１０に、直径１ｍｍの貫通孔１６が形成された中敷部材１２を入れて蓋をした。また、比較例として、上記カセット本体１０にバイオプシーシート（スポンジ）を入れて蓋をした。これらのカセット本体１０を、各々５個ずつ輪ゴムで束とし５束ずつ作製した。   The insole member 12 in which the through hole 16 having a diameter of 1 mm was formed was put on the cassette body 10 in which the through hole 14 having a diameter of 1 mm was formed on the bottom, and the lid was covered. As a comparative example, a biopsy sheet (sponge) was placed in the cassette body 10 and covered. Five of these cassette main bodies 10 were produced as bundles of five rubber bands each.

次に、中敷部材１２が入ったカセット本体１０の束の番号を１番から５番とし、バイオプシーシートの入ったカセット本体１０の束の番号を６番から１０番とし、それぞれの重量を測定した。測定結果が表１のＡで示される。   Next, the number of the bundle of the cassette body 10 containing the insole member 12 is numbered 1 to 5, the number of the bundle of the cassette body 10 containing the biopsy sheet is numbered 6 to 10, and the respective weights are measured. did. The measurement results are indicated by A in Table 1.

次に、自動包埋装置用のステンレス製籠に上記カセット本体１０の束をセットし、籠に蓋をして５分間水に浸漬した後、各束を水平に静かに取り出し１分間静置後、各々の束の重量を測定した。測定結果が表１のＢで示される。   Next, the cassette body 10 bundle is set in a stainless steel basket for an automatic embedding apparatus, the lid is covered and immersed in water for 5 minutes, and then each bundle is gently and horizontally removed for 1 minute. The weight of each bundle was measured. The measurement result is indicated by B in Table 1.

さらに、上記水試験後のカセット本体１０の束を上記ステンレス製籠に戻し、籠に蓋をし、自動包埋装置用の試薬（アセトン系）に５分間浸漬した後、各束を水平に静かに取り出し１分間静置後、各々の束の重量を測定した。測定結果が表１のＣで示される。   Further, the bundle of cassette bodies 10 after the water test is returned to the stainless steel basket, the lid is covered, immersed in a reagent for an automatic embedding apparatus (acetone system) for 5 minutes, and then each bundle is gently and horizontally After taking out to stand for 1 minute, the weight of each bundle was measured. The measurement results are indicated by C in Table 1.

上記各測定結果から、各束の液の持ち越し量の平均値を求めた。この平均値が表１のＢ−Ａ及びＣ−Ａの欄に示される。また、この平均値を５で除して、上記カセット本体１０の１個当たりの液の持ち越し量も求めた。   From the above measurement results, the average value of the carry-over amount of each bundle was determined. This average value is shown in the columns B-A and C-A in Table 1. Further, the average value was divided by 5, and the carryover amount of the liquid per one cassette body 10 was also obtained.

水を使用した試験においては、カセット本体１０の１個当たりの液の持ち越し量は、中敷部材１２を使用した場合が０．５０ｍｌであるのに対して、バイオプシーシートを使用した場合では０．６５ｍｌであった。   In the test using water, the carry-over amount per cassette body 10 is 0.50 ml when the insole member 12 is used, but is 0. 0 when the biopsy sheet is used. 65 ml.

一方、専用薬液（アセトン系 密度０．８５３８ｇ／ｍｌ）を使用した試験においては、カセット本体１０の１個当たりの持ち越し量は、中敷部材１２を使用した場合が０．３７１ｇ（０．４３ｍｌ）であるのに対して、バイオプシーシートを使用した場合では２．９５９ｇ（３．４７ｍｌ）であった。   On the other hand, in the test using the exclusive chemical solution (acetone density 0.8538 g / ml), the carry-over amount per cassette body 10 is 0.371 g (0.43 ml) when the insole member 12 is used. On the other hand, when the biopsy sheet was used, it was 2.959 g (3.47 ml).

Figure 0004797167
Figure 0004797167

このように、水を使用した試験では、中敷部材１２及びバイオプシーシートを使用した場合の持ち越し量に有意差は認められなかった。これに対して、専用薬液を使用した試験では、中敷部材１２を使用した場合には水の場合とほぼ同量の持ち越し量にとどまるが、バイオプシーシートを使用すると水の場合にくらべて約５倍の持ち越し量となった。中敷部材１２を使用した場合と比べると約８倍の薬液を持ち越している。これは水に比べ薬液の表面張力が小さいため、バイオプシーシートに浸透しやすいことが原因である。従って、表面張力の小さい薬液を使用する必要がある現在の組織標本作製工程においては、本発明にかかる中敷部材１２により薬液持ち越し量を大幅に削減することができる。   Thus, in the test using water, a significant difference was not recognized in the carry over amount when using the insole member 12 and the biopsy sheet. On the other hand, in the test using the exclusive chemical solution, when the insole member 12 is used, the carry-over amount is almost the same as that in the case of water. The carry-over amount was doubled. Compared to the case where the insole member 12 is used, the chemical solution is carried about 8 times. This is because the surface tension of the chemical solution is smaller than that of water, so that it easily penetrates into the biopsy sheet. Therefore, in the current tissue specimen preparation process where it is necessary to use a chemical solution having a low surface tension, the amount of the chemical solution carried over can be greatly reduced by the insole member 12 according to the present invention.

本発明にかかる包埋用カセットの構成例の斜視図である。It is a perspective view of the example of composition of the cassette for embedding concerning the present invention. 本発明にかかるカセット本体及び中敷部材の変形例の平面図である。It is a top view of the modification of the cassette main body concerning this invention, and an insole member. 本発明にかかるカセット本体及び中敷部材の変形例の他の平面図である。It is another top view of the modification of the cassette main body concerning this invention, and an insole member. 本発明にかかる包埋用カセットを使用した包埋工程の説明図である。It is explanatory drawing of the embedding process using the cassette for embedding concerning this invention.

符号の説明Explanation of symbols

１０ カセット本体、１２ 中敷部材、１４、１６ 貫通孔、１８ 組織片、２０ パラフィン処理装置、２２ パラフィン包埋処理プレート。   10 cassette body, 12 insole member, 14, 16 through hole, 18 tissue piece, 20 paraffin processing apparatus, 22 paraffin embedding plate.

Claims (4)

組織標本を作製するための包埋用カセットであって、
底面に複数の貫通孔またはスリットが形成され、前記底面に組織片が収容される、上面が開口した箱形のカセット本体と、
少なくとも表面が液体の非吸収性を有するとともに、前記カセット本体の底面と略同一の形状であり、前記開口から前記カセット本体に落とし込み、前記底面に収容される組織片の上に載せられて、包埋処理中の該組織片の変形を防止することが可能であって、貫通孔またはスリットが形成された、前記カセット本体から独立した板状の中敷部材と、
を備えることを特徴とする包埋用カセット。 An embedding cassette for producing a tissue specimen,
A plurality of through holes or slits are formed on the bottom surface, and a tissue piece is accommodated on the bottom surface, and a box-shaped cassette body having an open top surface;
Together with at least the surface has a non-absorbent liquid, a bottom and substantially the same shape of the cassette body, darken from the opening in the cassette body, it is placed over the tissue pieces accommodated in the bottom surface, follicle It is possible to prevent deformation of the tissue piece during the embedding process, and a plate-like insole member independent of the cassette body , in which a through hole or a slit is formed,
An embedding cassette comprising: 請求項１記載の包埋用カセットにおいて、前記中敷部材に形成された貫通孔またはスリットは、前記カセット本体の底面に形成された貫通孔またはスリットと略同一であることを特徴とする包埋用カセット。   2. The embedding cassette according to claim 1, wherein the through hole or slit formed in the insole member is substantially the same as the through hole or slit formed in the bottom surface of the cassette body. Cassette. 請求項１または請求項２記載の包埋用カセットにおいて、前記カセット本体と前記中敷部材とが樹脂製であることを特徴とする包埋用カセット。 3. The embedding cassette according to claim 1 or 2 , wherein the cassette body and the insole member are made of resin. 請求項３記載の包埋用カセットにおいて、前記カセット本体と前記中敷部材とを構成する樹脂がポリアセタールであることを特徴とする包埋用カセット。 4. The embedding cassette according to claim 3 , wherein the resin constituting the cassette body and the insole member is polyacetal.
JP2006009806A 2006-01-18 2006-01-18 Embedding cassette Active JP4797167B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006009806A JP4797167B2 (en) 2006-01-18 2006-01-18 Embedding cassette

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2006009806A JP4797167B2 (en) 2006-01-18 2006-01-18 Embedding cassette

Publications (2)

Publication Number Publication Date
JP2007192616A JP2007192616A (en) 2007-08-02
JP4797167B2 true JP4797167B2 (en) 2011-10-19

Family

ID=38448443

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006009806A Active JP4797167B2 (en) 2006-01-18 2006-01-18 Embedding cassette

Country Status (1)

Country Link
JP (1) JP4797167B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104048862A (en) * 2013-03-15 2014-09-17 徕卡生物系统努斯洛克有限公司 Processor for working on histological samples

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2391519B1 (en) * 2010-11-18 2013-06-21 María Esther IRIZAR URIBARRI CAPSULA-MOLD FOR THE PROCESSING OF BIOPSIES.

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3049537B2 (en) * 1995-02-03 2000-06-05 悌二 竹崎 Fixation support method for biopsy sample, fixation support agent and embedding cassette
NO315063B1 (en) * 2001-10-19 2003-06-30 Total Biopsy As Device and method of cassette for preparing biological samples
DK2002894T3 (en) * 2002-09-26 2012-04-23 Biopath Automation Llc Tissue sample arranging tool and method for arranging a tissue sample cassette

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104048862A (en) * 2013-03-15 2014-09-17 徕卡生物系统努斯洛克有限公司 Processor for working on histological samples
CN104048862B (en) * 2013-03-15 2018-03-06 徕卡生物系统努斯洛克有限公司 Processing equipment for handling tissue samples

Also Published As

Publication number Publication date
JP2007192616A (en) 2007-08-02

Similar Documents

Publication Publication Date Title
JP5259619B2 (en) Biopsy support with sectionable elastic foam material
AU2022201012B2 (en) Sectionable cassette and embedding frame with tissue immobilizing separable lid, and methods for preparing biopsy tissue samples
JP2011503519A5 (en)
US5817032A (en) Means and method for harvesting and handling tissue samples for biopsy analysis
JP2018526632A5 (en)
US6458598B1 (en) Process for preparing and presenting a tissue sample for histological study
WO2008112145A1 (en) Tissue sample support and orientation device
Wolfe Tissue processing
JP4797167B2 (en) Embedding cassette
US8999273B2 (en) Methods and devices for preparing microscopy samples
KR100458860B1 (en) Auxiliary Tool for Examination of Biopsy Specimen
US20170205320A1 (en) Method and apparatus for cell block preparation of cytology specimens
JP4592434B2 (en) Method for preparing cytological specimen and cytological specimen prepared thereby
JP2017053700A (en) Preparation method of sample for form observation, apparatus for liquid processing used therefor, and sample stand
JP4642498B2 (en) Cassette for medical examination and method of manufacturing cassette block using the cassette
DE202013012842U1 (en) Container for holding a material sample from a living organism
JP4667061B2 (en) Method for producing specimen flake for medical examination
JP3066792U (en) Filter paper for biospecimen adsorption
US11828690B2 (en) Cytology cell block preparation devices and methods of cell block prepartaion using same
CN112630243A (en) Application of wood slice as TEM grid, TEM grid and preparation method thereof
Onozato et al. Evaluation of a completely automated tissue-sectioning machine for paraffin blocks
US20140079604A1 (en) Apparatus for facilitating examination of tissue samples
JP2014202725A (en) Closely holding member of biological tissue, and container equipped with the same
Vogel One-step complete melting of paraffin tissue microarrays using stabilization bodies
Knott Imaging green fluorescent protein-labeled neurons using light and electron microscopy

Legal Events

Date Code Title Description
RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7422

Effective date: 20071119

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20071127

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20071120

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20081209

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20110106

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110118

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110322

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110412

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110610

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20110705

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20110714

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150