JP4717149B1 - 細胞選択装置、細胞選択方法、および、プログラム - Google Patents
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Abstract
【解決手段】本発明は、測定データに基づく細胞またはバイオマスの生産量、および、当該生産量がどの程度変動するかを示す変動値を細胞または条件ごとに設定し、設定された生産量および変動値に基づいて、複数の細胞または条件を組み合わせた場合の生産量および変動値の可変範囲を算出し、最適化手法を用いて、可変範囲における最適な組み合わせを算出する。
【選択図】図1
Description
以下、本発明の実施の形態の概要について図1〜図5を参照して説明し、その後、本実施の形態の構成および処理等について詳細に説明する。図1は、本実施の形態の基本原理を示すフローチャートである。
次に、本細胞選択装置100の構成について図6を参照して説明する。図6は、本実施の形態が適用される本細胞選択装置100の構成の一例を示すブロック図であり、該構成のうち本実施の形態に関係する部分のみを概念的に示している。
次に、このように構成された本実施の形態における本システムの処理の一例について、以下に図7乃至図16を参照して詳細に説明する。
まず、本実施の形態におけるバイオマス生産における細胞選択装置100の処理の詳細について図7を参照して説明する。図7は、本実施の形態における細胞選択装置100の処理の一例を示すフローチャートである。
次に、抗癌剤のスクリーニングにおける細胞選択装置100の処理の詳細について図8乃至図16を参照して説明する。図8は、本実施の形態における細胞選択装置100の処理の一例を示すフローチャートである。
さて、これまで本発明の実施の形態について説明したが、本発明は、上述した実施の形態以外にも、特許請求の範囲に記載した技術的思想の範囲内において種々の異なる実施の形態にて実施されてよいものである。
102 制御部
102a 設定部
102b 可変範囲算出部
102c 最適化部
106 記憶部
106a 測定データベース
Claims (11)
- 記憶部と制御部とを少なくとも備えた細胞選択装置であって、
上記記憶部は、
複数の条件で培養された細胞の増殖またはバイオマスの生産に関する測定データを上記細胞ごとに記憶する測定データ記憶手段、
を備え、
上記制御部は、
上記測定データ記憶手段に記憶された上記測定データに基づく上記細胞または上記バイオマスの生産量、および、当該生産量がどの程度変動するかを示す変動値を上記細胞または上記条件ごとに設定する設定手段と、
上記設定手段により設定された上記生産量および上記変動値に基づいて、複数の上記細胞または上記条件を組み合わせた場合の上記生産量および上記変動値の可変範囲を共分散に基づいて算出する可変範囲算出手段と、
最適化手法を用いて、上記可変範囲における一定の上記変動値の下で上記生産量を最大化する上記組み合わせを算出する最適化手段と、
を備えたことを特徴とする、細胞選択装置。 - 請求項1に記載の細胞選択装置において、
上記変動値は、
上記生産量の標準偏差であることを特徴とする、細胞選択装置。 - 請求項1または2に記載の細胞選択装置において、
上記最適化手段は、
最適化手法を用いて、上記可変範囲における一定の上記変動値の下で上記生産量を最大化する有効フロンティアに最も近い上記組み合わせを算出することを特徴とする、細胞選択装置。 - 請求項1乃至3のいずれか一つに記載の細胞選択装置において、
上記最適化手法は、
パレート最適化、遺伝的アルゴリズム、最小二乗法、または、適切な確率的および解析的最適化法であることを特徴とする、細胞選択装置。 - 請求項1乃至4のいずれか一つに記載の細胞選択装置において、
上記複数の細胞は、
複数の種類の上記細胞であることを特徴とする、細胞選択装置。 - 請求項1乃至4のいずれか一つに記載の細胞選択装置において、
上記複数の細胞は、
単一の種類の複数の上記細胞であることを特徴とする、細胞選択装置。 - 請求項1乃至6のいずれか一つに記載の細胞選択装置において、
上記細胞は、
微生物であることを特徴とする、細胞選択装置。 - 請求項1乃至6のいずれか一つに記載の細胞選択装置において、
上記細胞は、
がん細胞であることを特徴とする、細胞選択装置。 - 請求項1乃至8のいずれか一つに記載の細胞選択装置において、
上記測定データは、
上記細胞の増殖を50%に抑制する化合物の濃度であるGI50であり、
上記生産量は、
上記GI50の逆数であることを特徴とする、細胞選択装置。 - 記憶部と制御部とを少なくとも備えた細胞選択装置において実行される細胞選択方法であって、
上記記憶部は、
複数の条件で培養された細胞の増殖またはバイオマスの生産に関する測定データを上記細胞ごとに記憶する測定データ記憶手段、
を備え、
上記制御部において実行される、
上記測定データ記憶手段に記憶された上記測定データに基づく上記細胞または上記バイオマスの生産量、および、当該生産量がどの程度変動するかを示す変動値を上記細胞または上記条件ごとに設定する設定ステップと、
上記設定ステップにて設定された上記生産量および上記変動値に基づいて、複数の上記細胞または上記条件を組み合わせた場合の上記生産量および上記変動値の可変範囲を共分散に基づいて算出する可変範囲算出ステップと、
最適化手法を用いて、上記可変範囲における一定の上記変動値の下で上記生産量を最大化する上記組み合わせを算出する最適化ステップと、
を含むことを特徴とする、細胞選択方法。 - 記憶部と制御部とを少なくとも備えた細胞選択装置に実行させるためのプログラムであって、
上記記憶部は、
複数の条件で培養された細胞の増殖またはバイオマスの生産に関する測定データを上記細胞ごとに記憶する測定データ記憶手段、
を備え、
上記制御部において、
上記測定データ記憶手段に記憶された上記測定データに基づく上記細胞または上記バイオマスの生産量、および、当該生産量がどの程度変動するかを示す変動値を上記細胞または上記条件ごとに設定する設定ステップと、
上記設定ステップにて設定された上記生産量および上記変動値に基づいて、複数の上記細胞または上記条件を組み合わせた場合の上記生産量および上記変動値の可変範囲を共分散に基づいて算出する可変範囲算出ステップと、
最適化手法を用いて、上記可変範囲における一定の上記変動値の下で上記生産量を最大化する上記組み合わせを算出する最適化ステップと、
を実行させるためのプログラム。
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US13/702,215 US20130079900A1 (en) | 2010-06-07 | 2011-03-31 | Cell selecting apparatus, cell selecting method, and computer program product |
PCT/JP2011/058299 WO2011155253A1 (ja) | 2010-06-07 | 2011-03-31 | 細胞選択装置、細胞選択方法、および、プログラム |
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