JP4704083B2 - Microscope objective lens unit and objective lens adapter - Google Patents

Microscope objective lens unit and objective lens adapter Download PDF

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JP4704083B2
JP4704083B2 JP2005084263A JP2005084263A JP4704083B2 JP 4704083 B2 JP4704083 B2 JP 4704083B2 JP 2005084263 A JP2005084263 A JP 2005084263A JP 2005084263 A JP2005084263 A JP 2005084263A JP 4704083 B2 JP4704083 B2 JP 4704083B2
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objective lens
lens unit
insertion member
microscope objective
tip
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JP2006267433A (en
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良治 斎藤
慶寿 谷川
和彦 長
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Olympus Corp
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Description

本発明は、顕微鏡対物レンズユニットおよび対物レンズ用アダプタに関するものである。   The present invention relates to a microscope objective lens unit and an adapter for an objective lens.

従来、生体試料等の試料を拡大観察するために、顕微鏡が用いられている。顕微鏡に取り付けられる顕微鏡対物レンズユニットは、顕微鏡本体に着脱可能に取り付けられる鏡筒内に複数のレンズからなる無限遠光学レンズ群を備えており、最先端のレンズあるいは平行平板を試料に近接あるいは接触させて、焦点位置に配置された試料を拡大観察することができるようになっている（例えば、特許文献１参照。）。
特開２００５−０３１４２５号公報 Conventionally, a microscope is used for magnifying and observing a sample such as a biological sample. The microscope objective lens unit attached to the microscope is equipped with an infinite optical lens group consisting of multiple lenses in a lens barrel that is detachably attached to the microscope body. Thus, the specimen placed at the focal position can be enlarged and observed (for example, see Patent Document 1).
JP 2005-031425 A

しかしながら、通常の顕微鏡対物レンズユニットは、レンズを収容する鏡筒の外径が大きく、実験動物等の生体試料の表面下の状態を生きたままの状態（in vivo）で観察するには適していない。すなわち、生体試料の表面下の状態を観察するには、実験動物等の表皮や筋肉組織を切開し、目的の各種臓器等の生体試料の表面を切開して顕微鏡対物レンズユニットの先端面を近接させる必要があるが、顕微鏡対物レンズのサイズが実験小動物に比較して大きいために、生体試料の表面下の状態を観察するには、生体試料を大きく切開し、あるいは、大きな孔を開ける必要がある。   However, an ordinary microscope objective lens unit has a large outer diameter of a lens barrel that accommodates a lens, and is suitable for observing a living sample (in vivo) under a surface of a biological sample such as a laboratory animal. Absent. In other words, in order to observe the state of the surface of the biological sample, cut the skin or muscle tissue of a laboratory animal, cut the surface of the biological sample such as various target organs, and bring the tip of the microscope objective lens unit closer However, because the size of the microscope objective lens is larger than that of small experimental animals, in order to observe the state under the surface of the biological sample, it is necessary to make a large incision or open a large hole in the biological sample. is there.

この場合、生体試料表面の切開直後あるいは穿孔直後における観察は可能であるものの、切開によって生体試料に大きなダメージを与えてしまうため、長期間にわたる経時的な観察は困難である。観察後縫合し、次の観察時に再度切開する方法も考えられるが、生体試料にに与えるダメージを考えると、時間が経過するにつれて正常な状態での観察は困難になるという不都合がある。   In this case, although observation immediately after incision or immediately after perforation of the surface of the biological sample is possible, since the incision causes great damage to the biological sample, it is difficult to observe over time for a long time. A method of suturing after observation and incising again at the next observation is also conceivable, but considering the damage to the biological sample, there is an inconvenience that observation in a normal state becomes difficult as time passes.

本発明は上述した事情に鑑みてなされたものであって、実験小動物を始めとする哺乳類の細胞、筋肉等の生体組織、あるいは、心臓、肝臓、脳等の各種臓器の表面下の状態を生きたままの状態で、比較的長期間にわたって、かつ簡易に観察することを可能とする顕微鏡対物レンズユニットおよび対物レンズ用アダプタを提供することを目的としている。   The present invention has been made in view of the above-described circumstances, and is capable of living under the surface of mammalian tissues such as experimental small animals, living tissues such as muscles, or various organs such as the heart, liver, and brain. An object of the present invention is to provide a microscope objective lens unit and an objective lens adapter that enable easy observation over a relatively long period of time.

上記目的を達成するために、本発明は以下の手段を提供する。
本発明は、顕微鏡本体に着脱可能に取り付ける顕微鏡対物レンズユニットであって、光学的に透明な材質からなる針状の挿入部材を、先端に光軸に沿って突出状態に備え、焦点位置を挿入部材の先端外部に配置してなる顕微鏡対物レンズユニットを提供する。 In order to achieve the above object, the present invention provides the following means.
The present invention is a microscope objective lens unit that is detachably attached to a microscope body, and includes a needle-like insertion member made of an optically transparent material in a protruding state along the optical axis at the tip, and a focal position is inserted Provided is a microscope objective lens unit arranged outside the tip of a member.

本発明に係る顕微鏡対物レンズユニットを顕微鏡本体に取り付けることにより、針状の挿入部材が光軸に沿って突出状態に配置される。そして、顕微鏡対物レンズユニットを試料に近接させて、その先端の挿入部材を試料内部に刺し入れることにより、挿入部材の先端を試料の表面下に配置することができる。顕微鏡対物レンズユニットの焦点位置が挿入部材の先端外部に配置されているので、この状態で観察を行うことにより、試料の表面下の焦点位置に照明光あるいは励起光を結像させ、焦点位置からの反射光あるいは蛍光を観察することが可能となる。この場合において、試料に刺し入れる挿入部材が針状に形成されていることにより、少ない侵襲で試料の表面下の状態を観察することが可能となる。   By attaching the microscope objective lens unit according to the present invention to the microscope main body, the needle-like insertion member is arranged in a protruding state along the optical axis. Then, by bringing the microscope objective lens unit close to the sample and inserting the insertion member at the tip thereof into the sample, the tip of the insertion member can be disposed below the surface of the sample. Since the focal position of the microscope objective lens unit is arranged outside the tip of the insertion member, by observing in this state, the illumination light or excitation light is imaged at the focal position below the surface of the sample, and from the focal position. It is possible to observe the reflected light or fluorescence. In this case, since the insertion member to be inserted into the sample is formed in a needle shape, it is possible to observe the state below the surface of the sample with less invasiveness.

上記発明においては、前記挿入部材の先端に、光軸に直交する透過面を備えることとしてもよい。
このように構成することで、顕微鏡本体から顕微鏡対物レンズユニットに導かれてきた照明光あるいは励起光が挿入部材の先端に設けられた透過面を透過して、顕微鏡対物レンズユニットの外部に出射され、挿入部材の先端外部の焦点位置に結像させられる。そして、焦点位置から戻る反射光または蛍光を検出することにより、光軸方向の前方に配置される試料の表面下の状態を観察することができる。 In the said invention, it is good also as providing the transmission surface orthogonal to an optical axis at the front-end | tip of the said insertion member.
With this configuration, the illumination light or excitation light guided from the microscope body to the microscope objective lens unit is transmitted through the transmission surface provided at the tip of the insertion member, and is emitted to the outside of the microscope objective lens unit. The image is formed at a focal position outside the tip of the insertion member. And the state under the surface of the sample arrange | positioned ahead of an optical axis direction is detectable by detecting the reflected light or fluorescence which returns from a focus position.

また、上記発明においては、前記挿入部材の先端に、光軸に対して傾斜する反射面を備えることとしてもよい。
このように構成することで、顕微鏡本体から顕微鏡対物レンズユニットに導かれてきた照明光あるいは励起光が挿入部材の先端に設けられた反射面によって反射させられた後に挿入部材の先端外部において結像させられる。反射面は光軸に対して傾斜しているので、顕微鏡本体から顕微鏡対物レンズユニットに至る光軸に対して側方を観察することができる。 Moreover, in the said invention, it is good also as providing the reflective surface which inclines with respect to an optical axis at the front-end | tip of the said insertion member.
With this configuration, the illumination light or excitation light guided from the microscope body to the microscope objective lens unit is reflected by the reflecting surface provided at the distal end of the insertion member, and then imaged outside the distal end of the insertion member. Be made. Since the reflecting surface is inclined with respect to the optical axis, the side can be observed with respect to the optical axis from the microscope body to the microscope objective lens unit.

また、本発明は、顕微鏡対物レンズユニットの先端に取り付けられる対物レンズ用アダプタであって、光学的に透明な材質からなる針状の挿入部材と、該挿入部材を対物レンズユニットの先端の光軸上に取り付ける取付部とを備え、対物レンズユニットに取り付けられた状態で、対物レンズユニットの焦点位置が挿入部材の外部に配置される対物レンズ用アダプタを提供する。   The present invention also provides an objective lens adapter attached to the tip of a microscope objective lens unit, a needle-like insertion member made of an optically transparent material, and the insertion member as an optical axis at the tip of the objective lens unit. An objective lens adapter is provided that includes an attachment portion attached on the objective lens unit, the focus position of the objective lens unit being disposed outside the insertion member in a state of being attached to the objective lens unit.

この発明によれば、顕微鏡対物レンズユニットの先端に取付部を取り付けることにより、針状の挿入部材が光軸に沿って突出するように顕微鏡対物レンズユニットに設けられる。これにより、通常使用されている顕微鏡対物レンズユニットを用いて、少ない侵襲で試料の表面下の状態を観察することが可能となる。   According to the present invention, by attaching the attachment portion to the tip of the microscope objective lens unit, the needle-like insertion member is provided in the microscope objective lens unit so as to protrude along the optical axis. Thereby, it becomes possible to observe the state under the surface of a sample with few invasiveness using the microscope objective lens unit normally used.

上記発明においては、前記挿入部材の先端に、顕微鏡対物レンズユニットの先端に取り付けられたときに光軸に直交することとなる透過面を備えることとしてもよい。
また、上記発明においては、前記挿入部材の先端に、顕微鏡対物レンズユニットの先端に取り付けられたときに光軸に対して傾斜する反射面を備えることとしてもよい。 In the above invention, a transmission surface that is orthogonal to the optical axis when attached to the tip of the microscope objective lens unit may be provided at the tip of the insertion member.
Moreover, in the said invention, it is good also as providing the reflective surface which inclines with respect to an optical axis at the front-end | tip of the said insertion member, when attached to the front-end | tip of a microscope objective lens unit.

本発明によれば、簡易な構成により、少ない侵襲で試料の表面下の状態を観察することができるという効果を奏する。したがって、生体試料等の試料を生きたままの状態で比較的長期にわたり観察し続けることができる。   According to the present invention, it is possible to observe the state below the surface of the sample with a small amount of invasion with a simple configuration. Therefore, it is possible to continue observing a sample such as a biological sample for a relatively long period in a living state.

本発明の一実施形態に係る顕微鏡対物レンズユニット１について、図１および図２を参照して以下に説明する。
本実施形態に係る顕微鏡対物レンズユニット１は、図１に示されるように、複数のレンズからなる無限遠光学レンズ群２と、該無限遠光学レンズ群２を収容する鏡筒３と、該鏡筒３の先端に配置される針状の挿入部材４とを備えている。鏡筒３には、顕微鏡対物レンズユニット１を顕微鏡本体（図示略）に着脱可能に取り付けるための取付ネジ５が備えられている。 A microscope objective lens unit 1 according to an embodiment of the present invention will be described below with reference to FIGS. 1 and 2.
As shown in FIG. 1, a microscope objective lens unit 1 according to this embodiment includes an infinite optical lens group 2 composed of a plurality of lenses, a lens barrel 3 that houses the infinite optical lens group 2, and the mirror. And a needle-like insertion member 4 disposed at the tip of the tube 3. The lens barrel 3 is provided with an attachment screw 5 for detachably attaching the microscope objective lens unit 1 to a microscope main body (not shown).

前記無限遠光学レンズ群２は、例えば、４倍〜１０倍程度の低倍率のレンズ群であって、その作動距離は１０〜１６ｍｍ程度である。
挿入部材４は、例えば、ガラスのような光学的に透明な材質からなり、鏡筒３の先端に光軸Ｃの方向に沿って突出し、先端に向かって漸次先細になる錐台状に形成されている。挿入部材４の先端面は、光軸Ｃに直交する透過面４ａを構成し、該透過面４ａを通して顕微鏡対物レンズユニット１内に顕微鏡本体側から導かれてきた光を出射させるようになっている。 The infinite optical lens group 2 is, for example, a low-magnification lens group of about 4 to 10 times, and its working distance is about 10 to 16 mm.
The insertion member 4 is made of, for example, an optically transparent material such as glass, and is formed in a truncated cone shape that protrudes along the direction of the optical axis C at the tip of the barrel 3 and gradually tapers toward the tip. ing. The distal end surface of the insertion member 4 constitutes a transmission surface 4a orthogonal to the optical axis C, and emits light guided from the microscope main body side into the microscope objective lens unit 1 through the transmission surface 4a. .

また、挿入部材４の先端の透過面４ａの位置は、無限遠光学レンズ群２の焦点位置よりも若干、無限遠光学レンズ群２側に配されている。したがって、無限遠光学レンズ群２を介して導かれた照明光あるいは励起光は、挿入部材４を通過して透過面４ａから出射し、透過面４ａの若干外側において結像されるように構成されている。   Further, the position of the transmission surface 4 a at the tip of the insertion member 4 is slightly closer to the infinity optical lens group 2 side than the focal position of the infinity optical lens group 2. Therefore, the illumination light or the excitation light guided through the infinity optical lens group 2 passes through the insertion member 4 and is emitted from the transmission surface 4a, and is imaged slightly outside the transmission surface 4a. ing.

このように構成された本実施形態に係る顕微鏡対物レンズユニット１によれば、顕微鏡本体に取り付けることにより、顕微鏡本体の光軸Ｃに沿って延びるように挿入部材４を配置することができる。そして、図２に示されるように、観察対象である生体試料等の試料Ａの表面から挿入部材４を試料Ａに刺して透過面４ａを試料Ａの表面下に配置する。   According to the microscope objective lens unit 1 according to the present embodiment configured as described above, the insertion member 4 can be disposed so as to extend along the optical axis C of the microscope main body by being attached to the microscope main body. Then, as shown in FIG. 2, the insertion member 4 is inserted into the sample A from the surface of the sample A such as a biological sample to be observed, and the transmission surface 4 a is disposed below the surface of the sample A.

この状態で、顕微鏡本体に備えられた照明光源あるいは励起光源を作動させて、照明光あるいは励起光Ｌを顕微鏡対物レンズユニット１内に導き、挿入部材４の先端の透過面４ａから出射させて、透過面４ａの外側に結像させる。これにより、試料Ａの表面下に配されている観察位置に照明光あるいは励起光Ｌを照射してその反射光または蛍光を挿入部材４先端の透過面４ａから、顕微鏡対物レンズユニット１内に戻すことができる。   In this state, the illumination light source or the excitation light source provided in the microscope main body is operated, the illumination light or the excitation light L is guided into the microscope objective lens unit 1, and is emitted from the transmission surface 4a at the tip of the insertion member 4, An image is formed outside the transmission surface 4a. Thereby, illumination light or excitation light L is irradiated to the observation position arranged under the surface of the sample A, and the reflected light or fluorescence is returned into the microscope objective lens unit 1 from the transmission surface 4a at the tip of the insertion member 4. be able to.

本実施形態に係る顕微鏡対物レンズユニット１によれば、挿入部材４が、試料Ａに刺すことが可能な小径寸法に形成されているので、顕微鏡対物レンズユニット１の鏡筒３の外形寸法が大きくても、試料Ａを大きく切開等する必要がなく、低侵襲で、試料Ａの表面下の観察を行うことができるという効果がある。   According to the microscope objective lens unit 1 according to the present embodiment, since the insertion member 4 is formed with a small diameter that can be inserted into the sample A, the outer dimension of the lens barrel 3 of the microscope objective lens unit 1 is large. However, there is no need to make a large incision or the like on the sample A, and there is an effect that observation under the surface of the sample A can be performed with minimal invasiveness.

次に、本発明の第２の実施形態に係る顕微鏡対物レンズユニット１について、図３および図４を参照して以下に説明する。
本実施形態の説明において、上述した第１の実施形態に係る顕微鏡対物レンズユニット１と構成を共通とする箇所に同一符号を付して説明を省略する。 Next, a microscope objective lens unit 1 according to a second embodiment of the present invention will be described below with reference to FIGS.
In the description of the present embodiment, the same reference numerals are given to the portions having the same configuration as the microscope objective lens unit 1 according to the first embodiment described above, and the description is omitted.

本実施形態に係る顕微鏡対物レンズユニット１０は、挿入部材１１の構成において第１の実施形態に係る顕微鏡対物レンズユニット１と相違している。
本実施形態において、挿入部材１１は、図３に示されるように、その先端が、無限遠光学レンズ群１２の光軸Ｃに対して略４５°の角度で傾斜した反射面１１ａを構成している。これにより、無限遠光学レンズ群１２から延びる光軸Ｃは反射面１１ａにおいて略９０°の角度で折り曲げられている。また、挿入部材１１は、前記反射面１１ａにより略９０°曲げられた光軸Ｃ′に直交する透過面１１ｂを備えている。これにより、無限遠光学レンズ群１２から導かれてきた照明光または励起光Ｌ、挿入部材１１先端の反射面１１ａにおいて略９０°屈曲させられた後に、透過面１１ｂを透過して透過面１１ｂ外側において結像させられるようになっている。 The microscope objective lens unit 10 according to the present embodiment is different from the microscope objective lens unit 1 according to the first embodiment in the configuration of the insertion member 11.
In the present embodiment, as shown in FIG. 3, the insertion member 11 has a reflecting surface 11 a whose tip is inclined at an angle of approximately 45 ° with respect to the optical axis C of the infinity optical lens group 12. Yes. Thereby, the optical axis C extending from the infinity optical lens group 12 is bent at an angle of approximately 90 ° on the reflecting surface 11a. Further, the insertion member 11 includes a transmission surface 11b orthogonal to the optical axis C ′ bent by approximately 90 ° by the reflection surface 11a. As a result, the illumination light or excitation light L guided from the infinity optical lens group 12 is bent by approximately 90 ° at the reflection surface 11a at the tip of the insertion member 11, and then passes through the transmission surface 11b to be outside the transmission surface 11b. In this way, an image is formed.

このように構成された本実施形態に係る顕微鏡対物レンズユニット１０によれば、顕微鏡本体に取り付けることにより、第１の実施形態と同様にして、顕微鏡本体の光軸Ｃに沿って延びるように挿入部材１１を配置することができる。そして、図４に示されるように、観察対象である生体試料等の試料Ａの表面から挿入部材１１を試料Ａに刺して先端を試料Ａの表面下に配置する。   According to the microscope objective lens unit 10 according to the present embodiment configured as described above, it is inserted so as to extend along the optical axis C of the microscope main body by being attached to the microscope main body in the same manner as the first embodiment. The member 11 can be arranged. Then, as shown in FIG. 4, the insertion member 11 is inserted into the sample A from the surface of the sample A such as a biological sample to be observed, and the tip is disposed below the surface of the sample A.

この状態で、顕微鏡本体に備えられた照明光源あるいは励起光源を作動させて、照明光あるいは励起光Ｌを顕微鏡対物レンズユニット１０内に導き、挿入部材１１に入射させると、挿入部材１１の先端に設けられた反射面１１ａによって照明光あるいは励起光Ｌが反射され、挿入部材１１の側面に配置されている透過面１１ｂから出射させられる。顕微鏡対物レンズユニット１０の無限遠光学レンズ群１２の焦点位置は透過面１１ｂ近傍の外側に配置されているので、照明光または励起光Ｌを透過面１１ｂの外側に結像させ、試料Ａの表面下に配されている観察位置に照明光あるいは励起光Ｌを照射してその反射光または蛍光を挿入部材１１先端の透過面１１ｂから、顕微鏡対物レンズユニット１０内に戻すことができる。   In this state, when the illumination light source or the excitation light source provided in the microscope main body is operated, the illumination light or the excitation light L is guided into the microscope objective lens unit 10 and is incident on the insertion member 11, the tip of the insertion member 11 is reached. Illumination light or excitation light L is reflected by the provided reflecting surface 11 a and is emitted from the transmitting surface 11 b disposed on the side surface of the insertion member 11. Since the focal position of the infinity optical lens group 12 of the microscope objective lens unit 10 is arranged outside the vicinity of the transmission surface 11b, the illumination light or the excitation light L is imaged outside the transmission surface 11b, and the surface of the sample A Illumination light or excitation light L is irradiated to the observation position arranged below, and the reflected light or fluorescence can be returned into the microscope objective lens unit 10 from the transmission surface 11b at the tip of the insertion member 11.

本実施形態に係る顕微鏡対物レンズユニット１０によれば、挿入部材１１を、試料Ａに刺して、その刺し入れ方向に直交する方向を観察することができる。本実施形態においても、挿入部材１１が鏡筒３に対して十分に小径寸法に形成されているので、顕微鏡対物レンズユニット１０の鏡筒３の外形寸法が大きくても、試料Ａを大きく切開等する必要がなく、低侵襲で、試料Ａの表面下の、表面に沿う方向の観察を行うことができるという効果がある。   According to the microscope objective lens unit 10 according to the present embodiment, the insertion member 11 can be stabbed into the sample A and the direction orthogonal to the stabging direction can be observed. Also in this embodiment, since the insertion member 11 is formed with a sufficiently small diameter with respect to the lens barrel 3, even if the outer dimension of the lens barrel 3 of the microscope objective lens unit 10 is large, the sample A is largely incised or the like. Therefore, there is an effect that observation in a direction along the surface under the surface of the sample A can be performed with minimal invasiveness.

次に、本発明の一実施形態に係る対物レンズ用アダプタ２０について、図５を参照して以下に説明する。
本実施形態に係る対物レンズ用アダプタ２０は、図５に示されるように、上記第１の実施形態に係る顕微鏡対物レンズユニット１の先端に設けられたのと同様の挿入部材４と、該挿入部材４を固定する取付部２１とを備えている。 Next, an objective lens adapter 20 according to an embodiment of the present invention will be described below with reference to FIG.
As shown in FIG. 5, the objective lens adapter 20 according to the present embodiment includes the same insertion member 4 as that provided at the distal end of the microscope objective lens unit 1 according to the first embodiment, and the insertion. And a mounting portion 21 for fixing the member 4.

取付部２１は、図５に示されるように、挿入部材４を固定する略円錐状の部材であって、顕微鏡対物レンズユニット１の鏡筒３先端に設けられた雄ネジ２２に締結される雌ネジ２３を有するとともに、鏡筒３の先端面に突き当てられる突当段部２４を備えている。顕微鏡対物レンズユニット１の鏡筒３先端に設けられた雄ネジ２２に取付部の雌ネジ２３を締結し、鏡筒３の先端面に突当段部２４を突き当てることにより、挿入部材４が顕微鏡対物レンズユニット１の無限遠光学レンズ群２に対して位置決めされた状態に固定されるようになっている。   As shown in FIG. 5, the attachment portion 21 is a substantially conical member that fixes the insertion member 4, and is a female screw fastened to a male screw 22 provided at the distal end of the lens barrel 3 of the microscope objective lens unit 1. While having the screw | thread 23, the abutting step part 24 abutted by the front end surface of the lens-barrel 3 is provided. By fastening the female screw 23 of the mounting portion to the male screw 22 provided at the distal end of the lens barrel 3 of the microscope objective lens unit 1 and abutting the abutting step portion 24 against the distal end surface of the lens barrel 3, the insertion member 4 is moved. The microscope objective lens unit 1 is fixed in a state of being positioned with respect to the infinity optical lens group 2.

このように構成された本実施形態に係る対物レンズ用アダプタ２０によれば、顕微鏡対物レンズユニット１の先端に位置決め状態に取り付けることにより、挿入部材４の先端の透過面４ａの若干外側に顕微鏡対物レンズユニット１の無限遠光学レンズ群２の焦点位置を固定することができる。
したがって、本実施形態に係る対物レンズ用アダプタ２０を、先端に雄ネジ２２を有する通常の顕微鏡対物レンズユニット１の先端に取り付けるだけで、試料Ａに刺して表面下の状態を観察することができる顕微鏡対物レンズユニット１を構成することが可能となる。 According to the objective lens adapter 20 according to the present embodiment configured in this way, the microscope objective lens unit 1 is attached to the distal end of the microscope objective lens unit 1 in a positioning state, so that the microscope objective is slightly outside the transmission surface 4a at the distal end of the insertion member 4. The focal position of the infinity optical lens group 2 of the lens unit 1 can be fixed.
Accordingly, the objective lens adapter 20 according to the present embodiment can be inserted into the tip of a normal microscope objective lens unit 1 having a male screw 22 at the tip, and the sample A can be stabbed to observe the state below the surface. The microscope objective lens unit 1 can be configured.

なお、本実施形態に係る対物レンズ用アダプタ２０においては、挿入部材４の透過面４ａが顕微鏡対物レンズユニット１の無限遠光学レンズ群２の光軸Ｃに直交する形態のものを例に挙げて説明した。これに代えて、図６に示されるように、上記第２の実施形態に係る顕微鏡対物レンズユニット１０の挿入部材１１と同様に、先端に反射面１１ａを有し、光軸を９０°屈曲させる構造の挿入部材１１を備えるものとしてもよい。   In the objective lens adapter 20 according to the present embodiment, an example in which the transmission surface 4a of the insertion member 4 is orthogonal to the optical axis C of the infinity optical lens group 2 of the microscope objective lens unit 1 is taken as an example. explained. Instead, as shown in FIG. 6, like the insertion member 11 of the microscope objective lens unit 10 according to the second embodiment, the tip has a reflecting surface 11a and the optical axis is bent by 90 °. It is good also as what has the insertion member 11 of a structure.

また、上記実施形態においては、顕微鏡対物レンズユニット１の先端にネジ締結により取り付ける構造の対物レンズ用アダプタ２０を例示したが、これに代えて、図７に示されるように、顕微鏡対物レンズユニット１の鏡筒３先端に設けた円筒面２５に嵌合する筒状の嵌合部２６を備えることとし、接着剤、磁石、その他の方法によって鏡筒３に固定することとしてもよい。   Moreover, in the said embodiment, although the objective lens adapter 20 of the structure attached to the front-end | tip of the microscope objective lens unit 1 by screw fastening was illustrated, instead of this, as FIG. 7 shows, the microscope objective lens unit 1 It is good also as providing the cylindrical fitting part 26 fitted to the cylindrical surface 25 provided in the front-end | tip of this lens-barrel 3, and fixing to the lens-barrel 3 with an adhesive agent, a magnet, and another method.

なお、上記各実施形態においては、顕微鏡対物レンズユニットとして外径寸法の大きな鏡筒を有する場合について説明したが、これに代えて、鏡筒先端部の外径寸法を比較的小さくすることができる場合に、光軸に沿う方向と、その直交方向とで観察方向を切り替えたい場合がある。   In each of the above embodiments, the case where the microscope objective lens unit has a lens barrel having a large outer diameter has been described, but instead, the outer diameter of the tip of the lens barrel can be made relatively small. In some cases, it may be desired to switch the observation direction between a direction along the optical axis and a direction orthogonal thereto.

そのような場合に、図８に示されるように、鏡筒３０の先端部にアダプタ３１を取り付ける。アダプタ３１は、その角度を変更可能な反射板３２を内蔵し、鏡筒３０の先端部にワンタッチで取り付ける取付構造３３を備えている。アダプタ３１の先端面および側面には、光学的に透明な材質、例えば、ガラスからなる窓部３４が設けられている。取付構造３３は、例えば、図８に示す例では、鏡筒３０先端部の外面に設けられた周溝３３ａと、アダプタ３１に設けられ、前記周溝３３ａに嵌合する嵌合突起３３ｂとから構成されている。   In such a case, the adapter 31 is attached to the tip of the lens barrel 30 as shown in FIG. The adapter 31 includes a reflection plate 32 that can change its angle, and includes an attachment structure 33 that is attached to the distal end portion of the lens barrel 30 with one touch. A window portion 34 made of an optically transparent material, for example, glass is provided on the tip surface and side surface of the adapter 31. For example, in the example shown in FIG. 8, the mounting structure 33 includes a circumferential groove 33 a provided on the outer surface of the distal end portion of the lens barrel 30, and a fitting protrusion 33 b provided on the adapter 31 and fitted into the circumferential groove 33 a. It is configured.

図９に示されるように、鏡筒３０の先端部にアダプタ３１を取付構造３３により取り付け、反射板３２を傾斜させると、鏡筒３０の光軸Ｃが反射板３２によって屈曲させられて、アダプタ３１の側面に設けられた窓部３４方向に指向される。これにより、アダプタ３１の先端を試料内に刺し入れると、刺し入れ方向とは直交する方向に配される試料の表面下の状態を観察することができる。   As shown in FIG. 9, when the adapter 31 is attached to the distal end portion of the lens barrel 30 by the attachment structure 33 and the reflecting plate 32 is inclined, the optical axis C of the lens barrel 30 is bent by the reflecting plate 32, and the adapter It is directed in the direction of the window 34 provided on the side surface of 31. Thereby, when the tip of the adapter 31 is inserted into the sample, the state below the surface of the sample arranged in the direction orthogonal to the insertion direction can be observed.

また、本実施形態においては、取付構造３３が周溝３３ａと突起３３ｂとを嵌合させるものであるため、図１０に示されるように、鏡筒３０に対してアダプタ３１を光軸Ｃ回りに回転させることができる。これにより、鏡筒３０を固定したままの状態で、光軸Ｃに直交する観察方向を光軸Ｃの回りに全周にわたって切り替えることができる。   Further, in the present embodiment, since the mounting structure 33 fits the circumferential groove 33a and the protrusion 33b, the adapter 31 is moved around the optical axis C with respect to the lens barrel 30 as shown in FIG. Can be rotated. Thereby, the observation direction orthogonal to the optical axis C can be switched around the optical axis C over the entire circumference with the lens barrel 30 fixed.

また、鏡筒３０の光軸方向の観察を行いたい場合には、図１１に示されるように、反射板３２を折り畳むことで光軸Ｃ上から除外し、アダプタ３１先端の窓部３４を介して外部を観察することが可能となる。これにより、刺し入れ方向前方に配される試料の表面下の状態を観察することができる。
反射板３２は、ワイヤ（図示略）の押し引きにより揺動させることにしてもよく、また、磁石等の任意の駆動手段によって駆動することにしてもよい。 Further, when it is desired to observe the optical axis direction of the lens barrel 30, as shown in FIG. 11, the reflector 32 is removed from the optical axis C by folding, and through the window 34 at the tip of the adapter 31. It is possible to observe the outside. Thereby, the state under the surface of the sample arranged ahead in the insertion direction can be observed.
The reflector 32 may be swung by pushing and pulling a wire (not shown), or may be driven by an arbitrary driving means such as a magnet.

本発明の一実施形態に係る顕微鏡対物レンズユニットを示す模式図である。It is a mimetic diagram showing a microscope objective lens unit concerning one embodiment of the present invention. 図１の顕微鏡対物レンズユニットを用いた試料の観察を説明する模式図である。It is a schematic diagram explaining observation of the sample using the microscope objective-lens unit of FIG. 本発明の第２の実施形態に係る顕微鏡対物レンズユニットを示す模式図である。It is a schematic diagram which shows the microscope objective-lens unit which concerns on the 2nd Embodiment of this invention. 図２の顕微鏡対物レンズユニットを用いた試料の観察を説明する模式図である。It is a schematic diagram explaining observation of the sample using the microscope objective-lens unit of FIG. 本発明の一実施形態に係る対物レンズ用アダプタを示す縦断面図である。It is a longitudinal section showing an adapter for objective lenses concerning one embodiment of the present invention. 図５の対物レンズ用アダプタの変形例を示す縦断面図である。It is a longitudinal cross-sectional view which shows the modification of the adapter for objective lenses of FIG. 図５の対物レンズ用アダプタの他の変形例を示す縦断面図である。It is a longitudinal cross-sectional view which shows the other modification of the adapter for objective lenses of FIG. 顕微鏡対物レンズユニットの鏡筒の先端に取り付けるアダプタの変形例を示す縦断面図である。It is a longitudinal cross-sectional view which shows the modification of the adapter attached to the front-end | tip of the lens-barrel of a microscope objective lens unit. 図８のアダプタを鏡筒に取り付けた状態を示す縦断面図である。It is a longitudinal cross-sectional view which shows the state which attached the adapter of FIG. 8 to the lens barrel. 図９のアダプタを鏡筒に対して光軸回りに回転させた状態を示す縦断面図である。FIG. 10 is a longitudinal sectional view showing a state in which the adapter of FIG. 9 is rotated around the optical axis with respect to the lens barrel. 図９のアダプタ内の反射板を折り畳んだ状態を示す縦断面図である。It is a longitudinal cross-sectional view which shows the state which folded the reflecting plate in the adapter of FIG.

符号の説明Explanation of symbols

１，１０ 顕微鏡対物レンズユニット
４，１１ 挿入部材
４ａ，１１ｂ 透過面（透過面）
１１ａ 反射面
２１ 取付部 1,10 Microscope objective lens unit 4,11 Insert member 4a, 11b Transmission surface (transmission surface)
11a Reflecting surface 21 Mounting part

Claims (6)

顕微鏡本体に着脱可能に取り付ける顕微鏡対物レンズユニットであって、
光学的に透明な材質からなる針状の挿入部材を、先端に光軸に沿って突出状態に備え、焦点位置を挿入部材の先端外部に配置してなる顕微鏡対物レンズユニット。 A microscope objective lens unit that is detachably attached to the microscope body,
A microscope objective lens unit comprising a needle-like insertion member made of an optically transparent material in a protruding state along the optical axis at the tip, and a focal position disposed outside the tip of the insertion member. 前記挿入部材の先端に、光軸に直交する透過面を備える請求項１に記載の顕微鏡対物レンズユニット。   The microscope objective lens unit according to claim 1, wherein a transmission surface orthogonal to the optical axis is provided at a distal end of the insertion member. 前記挿入部材の先端に、光軸に対して傾斜する反射面を備える請求項１に記載の顕微鏡対物レンズユニット。   The microscope objective lens unit according to claim 1, further comprising a reflecting surface that is inclined with respect to an optical axis at a distal end of the insertion member. 顕微鏡対物レンズユニットの先端に取り付けられる対物レンズ用アダプタであって、
光学的に透明な材質からなる針状の挿入部材と、該挿入部材を対物レンズユニットの先端の光軸上に取り付ける取付部とを備え、対物レンズユニットに取り付けられた状態で、対物レンズユニットの焦点位置が挿入部材の外部に配置される対物レンズ用アダプタ。 An adapter for an objective lens attached to the tip of a microscope objective lens unit,
A needle-like insertion member made of an optically transparent material, and an attachment portion for attaching the insertion member on the optical axis at the tip of the objective lens unit, in the state of being attached to the objective lens unit, An objective lens adapter in which the focal position is arranged outside the insertion member. 前記挿入部材の先端に、顕微鏡対物レンズユニットの先端に取り付けられたときに光軸に直交することとなる透過面を備える請求項４に記載の対物レンズ用アダプタ。   5. The objective lens adapter according to claim 4, further comprising: a transmission surface that is orthogonal to the optical axis when attached to the distal end of the microscope objective lens unit at the distal end of the insertion member. 前記挿入部材の先端に、顕微鏡対物レンズユニットの先端に取り付けられたときに光軸に対して傾斜する反射面を備える請求項４に記載の対物レンズ用アダプタ。   5. The objective lens adapter according to claim 4, further comprising a reflective surface that is inclined with respect to the optical axis when attached to the distal end of the microscope objective lens unit at the distal end of the insertion member.
JP2005084263A 2005-03-23 2005-03-23 Microscope objective lens unit and objective lens adapter Expired - Fee Related JP4704083B2 (en)

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JP2005084263A JP4704083B2 (en) 2005-03-23 2005-03-23 Microscope objective lens unit and objective lens adapter
EP06005109A EP1705509B1 (en) 2005-03-23 2006-03-13 Scanning examination apparatus
DE602006009166T DE602006009166D1 (en) 2005-03-23 2006-03-13 Abtastuntersuchungsgerät
US11/375,042 US7436562B2 (en) 2005-03-23 2006-03-15 Scanning examination apparatus, lens unit, and objective-lens adaptor

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