JP4693198B2 - Biochip - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention, DNA, chip relates called biochip RN A, and in particular to improvements to the quality and manufacturing ease.
[0002]
[Prior art]
One of the DNA chip manufacturing methods, there is a method of adhering with a solution eg stamp method on a substrate such as a slide glass or silicon by a pin of DNA probe (hereinafter referred to as probe DNA). RNA is also produced by the same method.
[0003]
[Problems to be solved by the invention]
However, in this case, the target probe DNA is commercially available as a library or purified by itself, but it is often unclear whether or not it is the target DNA.
[0004]
Therefore, usually, before the chip is manufactured, electrophoresis is performed to check whether the probe solution is the target DNA. However, such work is troublesome twice, and there is a problem that it takes time and money.
[0005]
The object of the present invention is to solve the above-mentioned problems. It has an electrophoresis region and a hybridization region in one biochip, and it is easily confirmed that the probe solution is the target bio by performing electrophoresis. It is to provide a biochip that can be used.
[0006]
Hybridization means that a nucleic acid denatured into a single strand forms a hybrid with another single-stranded nucleic acid containing a complementary base sequence under appropriate conditions through a hydrogen bond specific to the sequence. Specifically, a nucleic acid having a known sequence is used as a probe, and a sample is examined for a complementary sequence as a target. At this time, by labeling the hybrid formed by the probe and the target, the complementary sequence in the sample can be detected and quantified.
[0007]
[Means for Solving the Problems]
In order to achieve such an object, the invention of claim 1 is a biochip in which a probe solution of DNA or RNA is attached on a substrate, and an electrophoretic region and a non-overlapping adjacent position on the substrate. and a hybridizing region, across both the area by attaching the probe solution, characterized in that it is configured so that the probe solution adhered to the electrophoretic region can be identified by electrophoresis.
[0008]
According to such a configuration, whether or not the molecule of the probe solution attached to the hybridization region is the target molecule can be easily confirmed by electrophoresis in the electrophoresis region.
[0009]
Further, the electrophoresis region and the region of hybridization can contact provided at a position adjacent to each probe solution, the probe solution, so attached to to span the electrophoretic region and hybridizing region, it requires only one position adhesion of There are advantages.
[0011]
DETAILED DESCRIPTION OF THE INVENTION
Hereinafter, the present invention will be described in detail with reference to the drawings. FIG. 1 is a block diagram showing an embodiment of a biochip according to the present invention. In this embodiment, a DNA chip is taken as an example. In the figure, an electrophoresis region A and a hybridization region B are prepared on a substrate 1, and a probe DNA solution is attached to each of the electrophoresis region A and the hybridization region B in pairs. In the figure, the probe DNA solution adhered to the hive Li die internalization region B by the solid line in ○ mark, also are shown, respectively probe DNA solution adhered to electrophoresis region A in dashed ○ mark.
[0012]
An electrophoresis gel is attached to the electrophoresis region A, and a probe DNA solution is spotted on the gel. A voltage is applied by means (not shown) to cause electrophoresis of the probe DNA. Electrophoretic reading can be performed using fluorescence emission or the like, as in normal electrophoresis.
[0013]
If it is confirmed (that is, identified) that the probe DNA of the cell is the target, then the sample solution is flowed and hybridization is performed in the same manner as in a normal DNA chip. In this way, a biochip of the target molecule can be easily produced.
[0014]
In addition, this invention is not limited to an Example. For example, biochips not just DNA, DNA that is also applicable in RN A. Further, after electrophoresis, the portion may be deleted by a laser or mechanical means. If deleted, the effect on gel hybridization is reduced.
[0015]
Further, the probe DNA solution may be attached so as to straddle both the electrophoresis region A and the hybridization region B as shown in FIG. 2 or FIG. The broken line portion is the attached DNA solution.
[0016]
Further, electrophoresis reading may be performed under a microscope instead of a camera. Moreover, the electrophoresis region A and the hybridization region B may be separated for each probe solution as shown in FIG. At the time of hybridization, it is only necessary to flow the test sample solution only to the portion of the hybridization region B.
[0017]
【The invention's effect】
As described above, according to the present invention, an electrophoresis region and a hybridization region are provided in one biochip, and electrophoresis is performed in the electrophoresis region to confirm that the target biochip is the target biochip. Can be easily manufactured.
[Brief description of the drawings]
FIG. 1 is a block diagram showing an embodiment of a biochip according to the present invention.
FIG. 2 is a diagram showing another embodiment of a biochip.
FIG. 3 is a view showing still another embodiment of the biochip.
FIG. 4 is a view showing still another embodiment of the biochip.
[Explanation of symbols]
1 Substrate A Electrophoresis region B Hybridization region

Claims (1)

A biochip having a DNA or RNA probe solution attached on a substrate,
Having an electrophoresis region and a hybridization region at adjacent positions on the substrate that do not overlap,
Across both the area by attaching the probe solution, biochip, characterized in that it is configured to be identified by the probe solution electrophoresis attached to the electrophoretic region.

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