JP4619071B2 - Processing equipment - Google Patents

Description

  In the present invention, the protein in the material is fragmented using reactive particles to improve the processing process and ensure the quality in fields such as washing, food production, and drug production. The technology for manufacturing products.

  Conventionally, a protein degrading technique has been used in the food production or medical fields. For example, in the method using fermentation, protein or starch is converted to alcohol or vitamin by the reaction of bacteria or enzymes.

  For example, in Patent Document 1, as an example in which protein degradation is used in the field of food production, malt and warm water are mixed in a charging tank, the protein is decomposed at a predetermined temperature for a predetermined time, and a mash (a mash-like one) is obtained. A method for producing a low-malt beer that controls the amount of free amino nitrogen in wort by adding a predetermined amount of proteolytic enzyme in the forming step and adjusting the amount added is disclosed.

  In Patent Document 2, as a technique for producing foods by removing allergenicity using proteolysis, the first hydrolysis is performed on the raw material protein in the range of 20-30% by endoprotease. And subjecting the obtained protein hydrolyzate to membrane fractionation using a membrane having a pore size of 1 nm to 5 μm, and increasing the degradation rate by 2 to 8% with a protease containing exoprotease in the permeate fraction, and A method for producing a protein hydrolyzate, in which the second hydrolysis is performed within a range where the final degradation rate is 25 to 35%, is disclosed.

Furthermore, in Patent Document 3, as an example in which proteolysis is used in the medical field, the blood in the human body is circulated extracorporeally, and the blood that is circulated extracorporeally is a voltage within a range where electrolysis does not occur or a voltage that causes electrolysis In the blood, for example, in the blood, by direct influence of voltage, current or indirect influence such as chlorine generated by electrolysis And methods of inactivating viruses that damage or destroy the RNA or reverse transcriptase or proteolytic enzymes present in the plasma component or the envelope or other important parts of the HIV virus on the surface of the HIV virus Is disclosed.
Japanese Patent Laid-Open No. 10-117760 JP 2001-333794 A JP-A-8-56657

  Although the methods described in Patent Documents 1 to 3 described above are all suitable as a method for decomposing a large amount of protein, there is a problem that an apparatus for realizing the method becomes large. For example, when an enzyme is used as a method for degrading a protein, it is necessary to cause a reaction at an appropriate temperature condition in order to increase the conversion efficiency. Therefore, a heat source and a temperature control device for causing the reaction are required. In this case, there is also a problem that it takes a long time for fermentation.

  On the other hand, in the method of degrading protein by applying a voltage or current to a protein-containing material, there is a problem that the effect of proteolysis cannot be obtained if the material does not have a fluidity gel or conductivity. There is. Particularly, when the protein-containing material is processed into a film shape, there is a problem that it is difficult to stably contact the current-carrying electrode for applying voltage or current without destroying the film-like material. .

  The present invention has been made in order to solve the above-mentioned problems, and the object of the present invention is to remarkably compare proteins contained in liquids, membranes, pharmaceutical raw materials, reagent raw materials and the like as compared with conventional ones. It is another object of the present invention to provide a processing method that can be easily and reliably decomposed, and a processing apparatus therefor.

In the present invention, positive ions H ₃ O ⁺ (H ₂ O) _n (n is 0 or a natural number) and negative ions O ₂ ⁻ (H ₂ O) _m (m is 0 or a natural number) are used as reactive particles. Means for generating, means for releasing the reactive particles, input means for inputting the name of the microorganism, protein or virus that is the inactivation target, and biochemical feature data relating to the inactivation target are stored in advance The inactivation target has, based on the result of the calculation means, the calculation means for collating the data of the storage means with the storage means to be compared with the data of the storage means and predicting the inactivation performance of the inactivation target the reactivity of the particles under conditions to fragment the protein is a processing device and a control means for controlling the release means to release to the inactivated object.

  According to the processing method and the processing apparatus of the present invention, proteins such as bacteria can be effectively and safely fragmented in the manufacturing process, which can greatly contribute to the production or washing of foods and medicines.

  The present invention is a processing method for fragmenting proteins contained in a liquid or film by releasing air containing particles reactive to the liquid or film. According to the treatment method of the present invention, the reactive particles can be diffused or sent out along the surface of the object by discharging the reactive particles in the air. The object can be reacted efficiently. Further, according to the treatment method of the present invention, proteins contained in the liquid or film-like substance can be fragmented easily and reliably without requiring a large-scale apparatus or the like as in the prior art. Here, “protein fragmentation” in the present invention refers to structural separation / decomposition by cleaving molecular bonds in the protein, and also includes decomposition accompanied by chemical modification. By fragmenting the protein, the molecular weight of the original protein is changed, and the original physical properties and functions are lost. This makes it possible to remove allergenicity in the material containing the protein, to reduce the growth ability of microorganisms, viruses or prions, to produce amino acids, and the like. Therefore, the treatment method of the present invention can be applied to a wide range of fields such as food production, pharmaceutical production, and reagent production. Further, according to the treatment method of the present invention, unlike the conventional method using voltage or current application, the protein contained therein is fragmented even in the case of a gel or a non-conductive material. Moreover, even if it is a membranous substance, there exists an advantage that protein fragmentation can be performed without destroying. In the treatment method of the present invention, since the material containing the protein to be fragmented is a liquid or a film-like material, an interaction such as oxidation on the surface of the material containing the protein is efficiently performed by the reactive particles. Is possible.

The reactive particles in the treatment method of the present invention refer to particles in which atoms or molecules are in a physically or chemically high energy state. As a method for generating the reactive particles, it is possible to apply discharge, excitation of electrons by an electric field, collision of charged particles accelerated by an electric field, etc., photoexcitation, application of kinetic energy, or the like. In addition, since the particles having the reactivity have a characteristic of being able to have a chemical reaction with an external organic chemical substance, they are oxidized or reduced, hydrolyzed and added directly or secondarily to proteins. It refers to a particle that can exert an action such as a reaction and has properties such as fragmenting, that is, decomposing, and changing the function of the protein. Specifically, the particles having reactivity include plasma, ions, radicals, nitrogen oxides (NO, NO _x ), sulfur oxides (SO _x ), hydrocarbons, hydrogen oxides (H ₂ O ₂ , HO _2). ), And accelerated electrons, accelerated protons, atomic hydrogen, high-energy atoms or molecules released from radioactivity, etc., among which at least one reactivity selected from plasma, ions and radicals Air containing particles is preferable, and air containing positive ions and negative ions as reactive particles is particularly preferable. In addition, in the production | generation method of the particle | grains which have the reactivity mentioned above, ozone may generate | occur | produce as a by-product. Since ozone has a long life and is persistent, it is desirable that the concentration be as low as possible. However, ozone may be contained in a small amount as long as it does not affect the human body or the like in the vicinity.

  The particles having at least one reactivity selected from the plasma, ions, and radicals can be generated by, for example, electrical excitation, and are contained in the material to be emitted because they have relatively short lifetime. Since the protein to be produced is quickly fragmented and disappears in a short time, it has a great effect on the release target, and the influence on other than the release target can be kept small. Therefore, it is not necessary to consider the influence on the outside even in an environment in which air containing reactive particles leaks to the outside of the release target.

Examples of positive ions and / or negative ions include H ₃ O ⁺ (H ₂ O) _n (n is 0 or a natural number) and / or O ₂ ⁻ (H ₂ O) _m (m is 0 or a natural number). It is preferable that these are the main components. Since it can be generated from atmospheric oxygen or moisture, the environmental load is low, and both of them react and become more active, such as hydrogen peroxide H ₂ O ₂ , hydrogen dioxide O ₂ H, hydroxy radicals, and OH. This is because active species are easily generated.

For example, on the surface of the discharge electrode, molecules such as oxygen (O ₂ ) and water (H ₂ O) in the air receive energy and are converted into reactive particles by plasma generated by creeping discharge. The positive and negative ions are mainly generated by the discharge phenomenon of the ion generating element, and normally, positive and negative ions can be simultaneously generated and released into the air by alternately applying positive and negative voltages. However, the method for generating both positive and negative ions used in the processing method of the present invention is not limited to this, and only one of the positive and negative voltages is applied to generate only one of the positive and negative ions first. After that, a reverse voltage can be applied to generate ions having a charge opposite to that of ions already sent out. The applied voltage required for generating these positive and negative ions may be in the range of 3.0 to 5.5 kV, preferably 3.2 to 5.5 kV, depending on the electrode structure.

The composition was of positive and negative ions generated by a discharge phenomenon molecular oxygen and / or water molecules present on the surface of the discharge element as a raw material, mainly as the positive ions of hydrogen ions of water molecules in the air is ionized by the plasma discharge H ⁺ This forms H ₃ O ⁺ (H ₂ O) _n (n is 0 or a natural number) by clustering with water molecules in the air by solvation energy. On the other hand, as negative ions, oxygen molecules or water molecules in the air are ionized by plasma discharge to generate oxygen ions O ₂ ⁻ , which are clustered with water molecules in the air by solvation energy, thereby O ₂ ⁻ (H ₂ O) _m (m is 0 or a natural number).

Then, these positive and negative ions released into the space surrounding the like bacteria, the chemical reaction (1), such as positive and negative ions is less at the surface of the bacteria, hydrogen peroxide H ₂ is the active species (2) O ₂ , hydrogen dioxide HO ₂ or hydroxy radicals · OH are generated.

H ₃ O ⁺ + O ₂ ⁻ → OH + H ₂ O ₂ (1)
H ₃ O ⁺ + O ₂ ⁻ → HO ₂ + H ₂ O (2)
The concentration of both positive and negative ions is 50 / m ^{3 to} 5 million / m ³ , preferably 500 / m ³ to 500,000 / m as the total number of positive and negative ions in the target region where the effect is exerted. ³ , more preferably 5000 / m ^{3 to} 50,000 / m ³ . When it is less than 50 / m ^3, there is a possibility that a sufficient sterilizing effect may not be obtained, whereas when it exceeds 5 million / m ³ , the concentration of ozone generated as a by-product increases, and further design Depending on the conditions, there is a possibility that the standard generally considered safe may be exceeded. Considering the stability of the processing apparatus described later, it is considered appropriate to avoid it unless there is a particular need. Here, the definition of the number of ions is a value obtained by counting small ions, and the critical mobility in air is 1 cm ² / V · sec.

  Whether air contains such reactive particles can be determined by a method of inspecting the gas composition by gas mass spectrometry inspection, gas concentration inspection, discoloration inspection, odor inspection, luminescence inspection, sound generation inspection, etc. It is done. The gas mass spectrometry test can use a conventionally known mass spectrometer, and the gas concentration test can be measured using a gas chromatography or an ion counter. In addition, the color change test and the odor test can be subjected to a sensory test such as a visual judgment or an olfactory test, and a color difference meter or an odor sensor can also be used. In addition, the light emission test and the generated sound test can be subjected to a sensory test such as a visual judgment or an auditory test, and an absorptiometer, spectroscope, optical sensor, illuminometer, microphone, or the like can be used.

  The reactive particles used in the treatment method of the present invention have a lifetime (that is, a lifetime in which the number of particles decreases logarithmically with time and decreases to a natural logarithm is defined as a lifetime). It is 0.1 microsecond to 3000 seconds, and it is desirable that the natural disappearance occurs. Preferably, the lifetime is 1 μs to 300 seconds. This is because when the lifetime of the particles having reactivity is less than 0.1 μsec, the particles drastically decrease during blowing, and the particles cannot reach the protein in the material sufficiently, and when the lifetime exceeds 3000 sec. There is a possibility that the particles do not disappear, the increase in concentration cannot be suppressed, and the stability of performance cannot be maintained. Here, the lifetime of the particles having the reactivity indicates a time in which the number of particles decreases logarithmically with respect to time after the generation of the particles and decreases to a natural logarithm. In the method of measuring the ion concentration by flowing a constant flow of air and arranging the wind tunnel between the generator and the discharge means described later, the length of the wind tunnel is changed to several types, and the number of ions is compared. Can be measured.

  By using air containing reactive particles having a lifetime of, for example, 0.1 μs to 3000 seconds, the reaction with the protein proceeds rapidly, and a predetermined stable effect can be obtained. Without this, a stable amount of gas can be released to the target location. Particles having at least one reactivity selected from plasma, ions, and radicals have a relatively short lifetime in space, and can be appropriately adjusted under known conditions so as to satisfy the lifetime in the above-described range. . Furthermore, particles having at least one reactivity selected from plasma, ions and radicals have a short lifetime in space and a short lifetime when in contact with a solid. Therefore, when contacting a cell, the cell protein is destroyed, but the possibility of destroying a gene inside the cell is low. Therefore, since it is difficult to cause genetic changes, there is a low possibility of generation of pathogenic bacteria due to various mutations and new microorganisms that produce allergen substances. Suitable for application.

  In the processing method of the present invention, when the protein-containing material is a liquid, the protein contained in the liquid is fragmented by releasing air containing reactive particles while circulating or stirring the liquid. Is preferred. By circulating or stirring, it is possible to release air containing particles reactive to the protein in the state where the protein in the liquid is sequentially exposed to the surface, and effectively fragment the protein in the liquid. This is because it becomes possible.

  In the treatment method of the present invention, it is preferable to form a protective layer on the surface of a membrane-like material or liquid containing protein after releasing air containing reactive particles. The protective layer may be in the form of a film (protective film), may be covered with a protective member (protective cover), or may be a bottle cap. By forming a protective layer in this way, the material after protein fragmentation can be protected while the growth ability of microorganisms, viruses, prions, etc. that have proteins in the material is reduced. The material is hardly denatured for a long time, and the quality of the material can be kept stable for a long time. As a material for forming the protective layer, a material that does not transmit air is desirable, and metal such as aluminum foil or iron, vinyl, organic chemical substances typified by plastic such as polytetrafluoroethylene (PTFE), and the like can be used.

  In the present invention, the protein contained in the release target is a protein that constitutes a microorganism, virus, or prion, and there is a treatment method that suppresses the growth of the treated microorganism, virus, or prion by fragmenting the protein. A preferred example thereof. This treatment method can reduce the growth ability of microorganisms and viruses such as bacteria, bacteria, and prions that are infectious proteins contained in the release target, and can be processed by applying it to the field of food production and pharmaceutical production. It becomes possible to improve the safety of later foods, pharmaceuticals, reagents and the like.

  The treatment method of the present invention can also remove allergenicity from the liquid or film by fragmenting the protein contained in the liquid or film. This utilizes the fact that allergen substances contained in a liquid or film-like substance are also formed from proteins.

  The protein-containing liquid or film-like material in the treatment method of the present invention is not particularly limited, but the protein-containing liquid or film-like material is any of the following (1) to (3): It is particularly useful when.

(1) Food raw material (2) Cleaning liquid or residue of cleaning liquid (3) Pharmaceutical raw material or reagent raw material When the liquid or film-like substance containing protein is a pharmaceutical raw material, the pharmaceutical raw material is a vaccine. Is particularly useful. When the target of protein fragmentation is a vaccine material, it suppresses the growth ability by fragmenting the protein of microorganisms or viruses contained in the vaccine material, and at the same time exerts the effect of a vaccine using the fragmented protein Thus, it is possible to achieve both the effect of ensuring immunity as a vaccine and reducing the possibility of infectious diseases rarely caused by the microorganism or virus.

<First Embodiment>
FIG. 1 is a diagram schematically showing a first embodiment of the processing method of the present invention. FIG. 1 shows step by step an example in which the processing method of the present invention is applied to a method for producing food (specifically, sea urchin) and the material containing the protein to be fragmented is a film-like material.

  In the first embodiment of the present invention, first of all, a kamaboko 1 as an object is appropriately arranged (FIG. 1 (a)), and the surface of the kamaboko 1 includes a dye, for example, using a spatula 3. A kneaded material containing fish meat is applied to form a film-like material 2 (FIG. 1 (b)).

Next, by releasing air containing reactive particles into the film-like material 2 formed on the sea urchin 1, the protein contained in the film-like material 2, which is a kneaded material containing fish meat, is fragmented ( FIG. 1 (c)). As the air containing the particles having reactivity, for example, air containing ions is used. More specifically, a processing device (not shown) of the present invention, which will be described later, is installed so that the distance between the kamaboko 1 and the discharging means is 30 cm, and the air is blown at a wind speed of 1 m / sec from the blowing pipe 4 of the discharging means. was carried out, kamaboko 1 of 100,000 ion concentration for positive ions at the surface / cm ^3, 100,000 for negative ions / cm ^3, it is possible to release time is released in 3 minutes condition.

  In this way, by releasing air containing reactive particles to the membrane-like material 2 on the kamaboko 1, the protein contained in the fish-kneading material constituting the membrane-like material 2 is fragmented to exist inside. It is possible to reduce the ability of microorganisms to grow. In addition, there is an advantage that the allergen substance composed of protein contained in the fish meat kneading material is destroyed and the influence on the human body is reduced. The air containing the particles having reactivity is not limited to those having ions as the particles having reactivity, and may of course contain plasma or radicals.

  In the example of the food manufacturing method shown in FIG. 1, as shown in FIG. 1 (d), after the release of the air containing the reactive particles, the film-like product 2 is further coated with the protective layer 6. You may do it. By coating with the protective layer 6, it is possible to protect the membrane 2 after the protein is fragmented so as not to come into contact with the atmosphere. In this case, even if the film-like material 2 contains bacteria, the ability of the bacteria to grow is suppressed by the release of the air containing the reactive particles, so that anaerobic bacteria and the like may grow inside the sea urchin 1. Nor. As a material for forming the protective layer 6, a material that does not transmit air is desirable, and vinyl, aluminum foil, and the like exemplified above can be used. FIG. 1 (d) shows an example in which vinyl is used as the protective layer 6 and applied onto the film-like object 2 using a spatula 7.

  The apparatus applicable to the manufacturing method of the example shown in FIG. 1 includes a means for generating reactive particles, a means for exposing a protein in a material containing protein to the surface of the material, and the exposed protein. A device having a mechanism for applying the protein-containing material to the object surface. The device for exposing the protein to the material surface may be used. Further, if desired, a mechanism for applying the protective layer 6 may be further provided.

<Second Embodiment>
FIG. 2 is a diagram schematically showing a second embodiment of the processing method of the present invention. FIG. 2 shows an example in which the treatment method of the present invention is applied to a cleaning method (specifically, tableware cleaning method), and the material containing the protein to be fragmented is a cleaning solution.

  In the second embodiment of the present invention, first, the tableware 11 is housed in a suitable frame 12 that is normally provided in a tableware cleaning device (FIG. 2A). In addition, in the tableware 11 shown to Fig.2 (a), the remainder 13 of a foodstuff remains on the surface.

  Next, the tableware 11 accommodated in the frame 12 is cleaned using the cleaning liquid 14. For example, the cleaning is performed for about 5 minutes using the cleaning liquid 14 ejected at a speed of 10 m / sec. By this washing process, the remaining food 13 remaining on the surface of the tableware 11 is removed.

And the air 15 containing the particle | grains which have the reactivity to the tableware 11 after washing | cleaning is discharge | released, and the protein contained in the washing | cleaning liquid 14 on the tableware 11 surface or its residue (not shown) is fragmented (FIG. 2 ( c)). As the air containing the particles having reactivity, for example, those containing plasma as the particles having reactivity can be used. Specifically, means for generating plasma by discharging in oxygen gas supplied from an oxygen cylinder, and means for emitting plasma so that O ₂ ⁺ has a concentration of about 0.01 ppm on the surface of tableware 11. It can implement using the apparatus provided with at least. Since O ₂ ⁺ is an ion, the lifetime is estimated to be a few seconds or less. However, it is possible to fill the cleaning device with the above ion by devising ventilation, and it remains on the surface of the tableware 11. It has the effect of fragmenting the protein contained in water or residue, and can suppress the growth of bacteria and the like. Therefore, even when the tableware 11 is stored in the cleaning device as it is, the surface of the tableware 11 can be kept clean. In addition, since the lifetime of O ₂ ⁺ is short, the concentration is reduced in a short period with respect to the outside, so that the effect of ignoring the outside can be obtained.

In the above-described second embodiment of the present invention, the air containing the particles having reactivity is not limited to the above-described one, but N ²⁺ , O ²⁻ , NO ²⁻ , CO ^2−. The same effect can be expected even if radical species such as ions and OH radicals or plasma is included.

<Third Embodiment>
FIG. 3 is a flowchart showing the processing steps of the third embodiment of the processing method of the present invention. FIG. 3 shows an example in which the treatment method of the present invention is applied to a pharmaceutical or reagent production method, and the material containing the protein to be fragmented is a pharmaceutical raw material or a reagent raw material. The present invention fragments microorganisms and viruses contained in the pharmaceutical raw material or reagent raw material by fragmenting proteins contained in the pharmaceutical raw material or reagent raw material by releasing air containing particles reactive to the pharmaceutical raw material or reagent raw material. Alternatively, a treatment method characterized by removing the ability to grow proteins is also provided.

  In the third embodiment of the present invention, first, the culture material and the microorganism are mixed (step 3-a). In the subsequent step, the mixture of the culture material and the microorganism is cultured to produce a chemical component (step 3-b). In the subsequent process, chemical components generated after the cultivation are extracted (step 3-c). And the air containing the particle | grains which are reactive with the extract (pharmaceutical raw material or reagent raw material) obtained at the said process is discharge | released (step 3-d). As a result, the protein contained in the extract is fragmented, and the proliferation ability of microorganisms, viruses, prions and the like that have the protein is reduced. Furthermore, a product can be completed by carrying out the aseptic packaging of the substance produced as mentioned above in the subsequent process, for example (step 3-e).

  In the third embodiment of the present invention described above, the ability to grow microorganisms, viruses, prions, etc. in pharmaceutical raw materials and reagent raw materials can be reduced by releasing air containing reactive particles. Can reduce the risk of infections caused by viruses, prions, etc.

  In the third embodiment of the present invention, the chemical component extraction step (step 3-c) and the release step of air containing reactive particles (step 3-d) are in reverse order. There may be. Further, the extraction step (step 3-c) may be omitted and replaced with a heating step or the like.

  Further, the third embodiment of the present invention can be used for drug production using, for example, genetically modified Escherichia coli. Specifically, a predetermined chemical substance is produced in the E. coli by culturing using the culture material and genetically modified E. coli. Thereafter, the components are extracted by centrifugation or the like, and the air containing reactive particles is released while the liquid is formed into a film, or circulated or stirred, and the protein contained in the film or liquid is decomposed. By this step, the cell membrane protein of living E. coli is decomposed and the growth of E. coli is stopped thereafter, so that the generated chemical substance can be used as a medicine or a medicine.

<Fourth embodiment>
FIG. 4 is a diagram schematically showing a fourth embodiment of the processing method of the present invention. FIG. 4 shows in a stepwise manner an example in which the treatment method of the present invention is applied to a method for producing food (specifically beef ham).

  In the fourth embodiment of the present invention, beef after first being heated, brewed and seasoned is divided and processed into ham chunks 21 having independent and appropriate sizes (FIG. 4 (a)). ). Next, the surface of the ham lump 21 is subjected to processing such as washing or applying an upper drug. FIG. 4B shows a state in which liquid (residue of cleaning liquid) or film-like material 22 has adhered to the surface of the ham lump 21 after the treatment.

Subsequently, a reactive particle is included to inactivate the protein contained in the liquid or film 22 attached to the surface of the ham lump 21 or the protein present on the surface of the ham lump 21 itself. Air is released (FIG. 4C). As air containing the particle | grains which have reactivity, a radical (desirably OH radical) is discharge | released, for example. A method for generating radicals may be a method for generating radicals directly by discharge, but is not limited thereto. For example, a method in which H ₂ O _2, that is, hydrogen peroxide is decomposed and converted into OH radicals may be used, or a method in which ionized gas is reacted on the material surface to generate OH radicals may be used. In FIG. 4C, reference numeral 23 denotes a radical emission port, and reference numeral 24 denotes a radical path. After the above steps, the ham chunk 21 is packaged with a bag (preferably a vacuum pack) 25 so that new protein does not adhere to the ham chunk 21 (FIG. 4D).

  According to the fourth embodiment of the treatment method of the present invention, not only inactivates proteins possessed by microorganisms and viruses such as bacteria and bacteria, but also prions causing BSE (bovine spongiform encephalopathy). In principle, it is also possible to target a kind of protein called as a target for decomposition. In this case, it is possible to use this manufacturing technique in order to remove the prion slightly remaining on the food itself on the surface and enhance the safety of eating habits.

  The present invention also provides a processing apparatus for carrying out the above-described processing method of the present invention. The processing apparatus of the present invention includes means for generating reactive particles, means for exposing proteins in a material containing protein to the material surface, and means for releasing particles reactive to the exposed proteins. Are basically provided. Such a processing apparatus of the present invention releases a reactive particle while moving a material containing a protein to be fragmented to a location facing a gas and sequentially exposing the protein contained in the material to the surface of the material. be able to. This makes it possible to efficiently fragment proteins contained in the material.

  In the treatment apparatus of the present invention, the reactive particles preferably have a property of causing any reaction selected from oxidation, reduction, hydrolysis, and addition reaction as described above in the treatment method of the present invention. . Further, as described above, it is desirable that the reactive particles have a property of spontaneous extinction, and the lifetime is more preferably 0.1 μsec to 3000 seconds. The reactive particles are preferably at least one selected from plasma, ions and radicals.

  As a means for generating reactive particles in the treatment apparatus of the present invention, discharge means that have been widely used in the past to generate air containing the above-described reactive particles can be used as appropriate. It is not limited. For example, there may be mentioned those using various discharge elements such as creeping discharge elements, corona discharge elements, and plasma discharge elements, and elements that emit ultraviolet rays or electron beams. The shape and material of the electrode in the discharging means are not particularly limited, and any conventionally known appropriate material can be selected.

  FIG. 5 is a diagram showing a simplified discharge means 31 preferably used in the processing apparatus of the present invention. The discharge means 31 includes, for example, a dielectric 32 having a rectangular cross section, a discharge electrode 33 formed on one surface of the dielectric 32, a counter electrode 34 embedded in the dielectric 32, and a power source 35. Basically equipped. As the dielectric 32, for example, a material having a size of about 1 cm × 3 cm formed of alumina can be suitably used. In the discharge means 31, the discharge electrode 33 and the counter electrode 34 are formed to have an appropriate interval (for example, 0.2 mm). A high-voltage pulse power supply can be used as the power supply 35 and is electrically connected to the discharge electrode 33 and the counter electrode 34.

  When a creeping discharge element as shown in FIG. 5 is used, whether a positive ion or a negative ion is generated at a certain moment depends on whether the voltage applied to the electrode of the discharge element is positive or negative. It depends on. That is, when a negative voltage is applied to the electrode, the electrode is negatively charged, so that water vapor present in the air is charged and becomes negative ions. For this reason, a large amount of negative ions are contained in the air. Conversely, when a positive voltage is applied to the electrode, water vapor present in the air is charged and becomes positive ions. For this reason, the air contains a large amount of positive ions. Specifically, a positive and negative high voltage pulse voltage (frequency: 60 Hz, peak voltage: about 2 kV) is generated from the high voltage pulse power source and applied between the electrodes. Moreover, you may make it produce a positive ion and a negative ion alternately by making the voltage applied to an electrode into alternating current.

  As an example of the means for exposing the protein to the material surface in the processing apparatus of the present invention, one having a mechanism for applying the protein-containing material to the object surface can be mentioned. By having such a mechanism, a protein-containing material is formed into a film-like material, and then air containing particles reactive to the same is released to fragment the protein in the film-like material. it can. Such a processing apparatus can be suitably used, for example, to implement the first embodiment of the processing method of the present invention described above with reference to FIG. The mechanism for applying the protein-containing material to the surface of the object may be realized by applying a conventionally known appropriate mechanism.

  In addition, when the material containing the protein is a liquid, examples of means for exposing the protein to the material surface in the treatment apparatus of the present invention include those having a mechanism for circulating or stirring the liquid. . FIG. 6 is a diagram schematically showing a processing apparatus 41 of a preferred example of the present invention. In the processing device 41, the discharge means 31 described above, the container 43 in which the liquid 44 is accommodated in the casing 42, and the air containing the reactive particles generated by the discharge means 31 are converted into the liquid 44. Discharging means (not shown) for discharging. A stirring bar 45 is placed on the bottom of the container 43, and the liquid 44 stored in the container 43 is stirred by the stirring bar 45 so that the surface of the liquid 44 is sequentially replaced. As indicated by the white arrows in the figure, by releasing air containing reactive particles on the surface of the liquid 44, the proteins sequentially exposed on the surface of the liquid 44 are fragmented and contained in the liquid 44. Can be efficiently inactivated.

  The apparatus shown in FIG. 6 is configured to stir the liquid. However, as a means for exposing the protein to the surface of the liquid, it is needless to say that a mechanism for circulating the solution using a pump may be used.

  As the means for releasing the reactive particles (release means) in the processing apparatus of the present invention, it is possible to flow so that the air containing the reactive particles generated by the discharge means can be released to the discharge target. There is no particular limitation as long as it is possible. For example, a discharge unit including a motor and a fan attached to the shaft of the motor, and having a mechanism that rotates the fan to blow air by driving the motor can be preferably used.

  The present invention also provides a means for generating reactive particles (for example, a discharge means), a means for contacting or spraying a washing liquid containing protein to the object surface, and a reactivity for the object surface after the contact or spraying. There is also provided a cleaning device comprising means for discharging the particles it has (discharge means). According to such a cleaning apparatus of the present invention, it can be preferably applied to decompose protein components adhering to the cleaning surface and form a clean surface. For example, the above-described second embodiment of the processing method of the present invention is described. Can be used particularly preferably.

  In the cleaning apparatus of the present invention, as means for contacting or spraying the cleaning liquid containing protein on the surface of the object, for example, a mechanism for spraying hot tap water on the object using a pump, water containing a surfactant is mixed in the water This can be realized by applying an appropriate known mechanism such as a mechanism for spraying the water on an object with a post sprayer.

  In addition, as the discharge means and the discharge means in the cleaning apparatus of the present invention, those described above in the processing apparatus of the present invention can be used as appropriate.

  FIG. 7 is a diagram conceptually illustrating another example of the processing apparatus 51 of the present invention. The processing apparatus 51 of the example shown in FIG. 7 includes a means for generating reactive particles (discharge means 52), a means for releasing reactive particles (not shown), and a microorganism that is an inactivation target. , Input means 53 for inputting information relating to protein or virus, storage means 54 for storing biochemical feature data relating to the inactivated object in advance, data entered in the input means are collated with data in the storage means, Based on the result of the calculation means 55 for predicting the inactivation performance of the inactivation target, and the result of the calculation means, the particles having reactivity under the condition that fragments the protein of the inactivation target are fragmented as the inactivation target. Control means 56 for controlling the discharge means so as to discharge is basically provided.

  In the processing apparatus 51 of the example shown in FIG. 7, the discharge means 52 and the discharge means may be appropriately used as described above.

  The input means 53 is a means for inputting information on microorganisms, proteins or viruses that are inactivation targets. Here, examples of the information to be input include the names of microorganisms, proteins, and viruses. If the microorganism, protein, or virus that is the inactivation target is not stored in the storage means, the name of a similar microorganism, protein, or virus that is already stored is input.

  The storage means 54 is means for previously storing biochemical feature data related to the inactivated object. The storage means 54 is realized as a database that can access information desired for the task. Examples of biochemical feature data related to the inactivated object include data on proteins such as various microorganisms and viruses and proteins such as prions, presence or absence of capsules, presence or absence of a pentaglycine cross-linked structure, presence or absence of ticoic acid Alternatively, data on at least one of an amount, a metabolizing type, oxygen utilization characteristics, an amount of catalase, an amount of cytochrome, the presence or absence of spore formation, a pigment production amount, a cell structure, a survival mechanism, and the like can be mentioned.

  The calculating means 55 is a means for collating the information input by the input means 53 with the data in the storage means 54 and predicting the inactivation performance of the inactivated object. That is, the corresponding data stored in the storage means 54 is called in conjunction with the input information, and from this data, the air containing the reactive particles generated by the processing device 51 of the present invention is not detected. It has the function of predicting the inactivation performance of microorganisms, proteins, and viruses to be activated, and obtaining release conditions suitable for the inactivation operation.

  Based on the result of the calculation means 55, the control means 56 sets the release means so as to release air containing reactive particles to the inactivation target under conditions that fragment the protein of the inactivation target. It is a means to control. In the processing apparatus 51 of the example shown in FIG. 7, the control unit 56 generates particles having reactivity based on a result determined by the calculation unit 55 according to a predetermined setting, that is, either automatic operation or manual operation. Choose whether to automatically release the air containing air or to manually activate the air release containing the reactive particles. In the case of automatically releasing air containing particles having reactivity, the control means 56 sets various conditions by the discharge means according to the result predicted by the calculation means 54 and operates them.

  According to the processing apparatus 51 having the configuration shown in FIG. 7, the inactivation is actually tested by modeling the inactivation effect and the relationship between the configurations of each cell with a predetermined relational expression. Even if it is not a cell, it is possible to obtain a cell configuration derived from the type from a database or the like and predict the inactivation rate. And, for all existing bacteria, viruses, and proteins, even for unknown bacteria, viruses, and proteins, predict protein properties from existing databases from existing subjects, etc., and obtain degradation performance with less error And inactivation can be performed with high accuracy. Therefore, by using the processing apparatus 51 having the configuration shown in FIG. 7, the present apparatus can be used not only for safety of eating habits but also for sterilization of living space, etc. It becomes possible to do.

  It should be noted that the input means 53 in the processing device 51 shown in FIG. 7 is an appropriate publicly known means realized so as to be able to input information so as to be linked with the information processing in the storage means 54 and the calculation means 55 of the processing device 51. To be realized. The storage unit 54, the calculation unit 55, and the control unit 56 can be realized by, for example, a central processing unit (CPU) or a microcomputer. In addition, the processing device 51 shown in FIG. 7 preferably releases the information input by the input means 53, the data stored in the storage means 54 that has been called for business, and the air containing the manually reactive particles. This is realized so as to have a display means for displaying information to be presented to the operator.

  Hereinafter, test data when air containing particles having reactivity with an object is released is disclosed with respect to the processing method and the processing apparatus of the present invention. This test is an evaluation test of the bactericidal performance exhibited by air containing particles having reactivity generated by electric discharge against attached bacteria.

<Experimental example 1>
The test was conducted under the following conditions.

In the test method, the bacterium was suspended in a PBS buffer solution (pH = 7.4), and then applied on the agar medium 64 formed in the tray 63, followed by a predetermined treatment (a positive treatment generated by the discharging means, Releases ions H ₃ O ⁺ (H ₂ O) _n (n is 0 or a natural number) and negative ions O ²⁻ (H ₂ O) _m (m is 0 or a natural number) and puts them on the agar medium. After the natural diffusion of the ions.), The culture was performed at 37 ° C. for 72 hours, and the number of colonies was counted. As a first test, in order to investigate the disinfection performance of the discharge gas to the adherent bacteria, staphylococcus (Staphylococcus), Enterococcus malodolatus (Enterococcus), Sartina flava (Sarcina flava), Micrococcus, Using Rosecus (Micrococcus roseus), the bacteria were applied on the agar medium by the above-described method, and further cultured for 8 hours (37 ° C.) to form bacterial colonies.

Subsequently, as shown in FIG. 8, a device 61 having a discharge means 31 and a discharge means (not shown) in a casing 62 is used, and oxygen molecules and / or water molecules present on the surface of the discharge element are used as raw materials. Both positive and negative ions were generated by the discharge phenomenon, and air containing hydrogen peroxide H ₂ O ₂ , hydrogen dioxide O ₂ H, or hydroxy radical / OH generated as a result of the reaction as described above was released to the target. . The case 62 has a size of 21 cm × 14 cm × 14 cm. In such a case 62 of the processing apparatus 61, a tray 63 containing the agar medium 64 coated with the bacteria is sequentially placed, and air containing reactive particles as indicated by white arrows is provided. Released and exposed to air containing reactive particles, allowing ions to spread through the agar medium. Ion concentration, positive and negative ions on the agar medium has a respective about 3,500 / cm ³ (although measurement of the concentration of small ions to limit mobility as 1cm ² / V · cm), the ozone concentration is less than 0.01ppm Met. The test box was not provided with a fan, and ions were exposed by natural convection and natural diffusion.

  In the above test, the culture was continued at 37 ° C. for 72 hours, and the number of colonies and the state were observed. FIG. 9 is a graph showing the results. As shown in FIG. 9, when air containing ions as reactive particles was released to the bacterium, the number of colonies (CFU; Colony Forming Unit) obtained after the culture decreased as the release time increased. From this, it can be seen that the ions have the effect of sterilizing the attached bacteria. In FIG. 9, it is shown that the speed or degree of inactivation varies depending on the bacterial species. The reason for this difference is that the cell structure (cell membrane material, cell surface and internal state, survival method, etc.) differs depending on the bacterial species, and the cell resistance to plasma, ions, radicals, etc. is different. it is conceivable that.

  Here, the results of comparison of the cell walls in the above four types of bacteria are shown in Table 1.

  Table 1 shows typical elements constituting peptidoglycan protein, tycoic acid, polysaccharides, and the like, which are main constituents of bacteria, and cell characteristics. In the table, + indicates that the property is large,-indicates that the property is small, and +/- indicates that the property is intermediate.

The items in Table 1 are described next.
-The capsular membrane is a membrane made of polysaccharide. For example, bacteria with strong pathogenicity are said to have capsular polysaccharide outside the peptidoglycan layer.
-A pentaglycine bridge | crosslinking structure (5-Gly-cross bridges in cell wall) is one of the structures which comprise a cell wall.
Tychoic acid is contained in the cell wall and is a compound of alcohol and phosphate group.
・ Metabolism is a method of ingesting substances that are raw materials to build cell components, produce energy, or release by-products.
・ About oxygen utilization, it is an item about what kind of air environment bacteria prefer.
Catalase is an enzyme that breaks down hydrogen peroxide into oxygen and water and functions as an antioxidant.
Cytochrome is a kind of heme protein containing heme iron having a redox function.
・ Spore formation refers to the property that bacteria are encased in shells.
-Pigment production indicates the property of producing pigment itself in the cell and accumulating / holding it in the cell.

  In the graph shown in FIG. 9, under the same test conditions, Sartina flavae and Micrococcus roseus require relatively inactivation time, whereas Enterococcus malodolatus and Staphylococcus rapidly inactivate. Progressing.

  In FIG. 9, when the processing time is 100 minutes as an index, the slowest inactivation is followed by the order of sarcina flavoura, micrococcus roseus, staphylococcus, enterococcus malodoratus. Comparing this difference in the rate of inactivation with Table 1, the saltina flav has many characteristics of catalase, spore formation, and pigmentation, and also has an air-resistant property, so it exists in the air. It is thought that it has a property that can easily withstand highly reactive substances (such as ozone, oxygen, and ions), and a model in which inactivation does not proceed most is conceivable. Micrococcus roseus has many characteristics of catalase, cytochrome, spore formation, and pigment formation, and is therefore considered to exhibit the slow inactivation property next to the saltinoflavor.

  Staphylococcus, on the other hand, has a high amount of catalase and cytochrome, but does not form spores, has little pigmentation, and is a conditional anaerobic bacterium. Therefore, highly reactive substances (ozone, oxygen, It is considered that the two types exhibit properties that are difficult to withstand ions and the like.

  Secondly, Enterococcus malododoratus has less defense mechanisms such as catalase, cytochrome, spore formation and pigmentation, and is a conditionally anaerobic bacterium. , Oxygen, ions, etc.) are expected to have a property that is difficult to withstand, and in fact, the most inactivated result has been obtained.

  In the above discussion, it is expected that aerobicity is highly resistant to inactivation for oxygen utilization, and that catalase, cytochrome, spore formation, and pigmentation are expected to be highly resistant to inactivation. The trend was confirmed.

  On the other hand, the effects of other items such as capsule, pentaglycine cross-linked structure, tycoic acid, and metabolites cannot be clarified in this study. It is possible to confirm.

  As shown above, by examining the structure of the cells in the fungus, by the air containing reactive particles such as ions, plasma, ozone, radicals, or even chemicals having, for example, oxidation and reduction action, Control of cell inactivation is possible. In addition, by modeling the relationship between the effect of inactivation and the structure of each cell using a predetermined calculation formula, even if the cell is not actually tested for inactivation, It is possible to obtain the configuration etc. from a database etc. and predict the inactivation speed. Then, using this, the processing device 51 described above with reference to FIG. 7 can be realized.

<Experimental example 2>
Penicillium chrysogenum, Stachybotrys chartarum, Aspergillus versicolor, Aspergillus versicolor, Psicum versicolor An experiment similar to Experimental Example 1 was performed on (Cladosporium herbarum). FIG. 10 is a graph showing results for Penicillium chrysogenum, FIG. 11 is Stachybotryschartorum, FIG. 12 is Aspergillus versicolor, FIG. 13 is Penicillium cammannti, and FIG. As a result of experiments, it was revealed that the number of colonies obtained after culturing (CFU: Colony Forming Unit) decreases as the release time becomes longer when ions are released to mold.

<Experimental example 3>
Since mold is a spore-forming fungus that is resistant to thermal shock and physical attack, when it begins to form spores, it may block ions and prevent bacterial protein degradation according to the present invention. There is. Therefore, after culturing Aspergillus versicolor (Koji mold) and Cladosporium herbalum (Clavisporium herbarum) on the petri dish, the spores were formed once, In the same manner as in Experimental Example 1, ions were released for 4 hours, and the change was observed. FIG. 15 is a photograph showing the results for Aspergillus bersicolor and Cladosporium herbalem in Experimental Example 3. As shown in FIG. 15, as a result of the above-mentioned experiment, it was found that the release of ions inhibited the formation of further spores, and the mold colonies disappeared.

  Therefore, the same experiment was conducted for molds other than the above. The results are shown in Table 2. As shown in Table 2, it was found that inhibition of sporulation and disappearance of colonies were observed in the case of other molds as well. From this, it can be seen that ions have the effect of sterilizing Gram-positive cocci and fungi, and the adherent bacteria of both.

<Experimental example 4>
Changes in protein due to release of air containing ions as reactive particles to attached bacteria were investigated. As an experimental method, ions were released in the same manner as in Experimental Example 1 to a plurality of agar media each coated with Enterococcus malodoratus, and after release, 15, 30, 60, 90, 120 Membrane proteins at minutes of 240 minutes, 240 minutes, 480 minutes, and 960 minutes were extracted and subjected to two-dimensional electrophoresis by SDS-PAGE. FIG. 16 is a photograph showing the results of Experimental Example 4. As shown in FIG. 16, by releasing ions, many protein fragments that appeared as pathological phenomena were observed. This fragmentation and aggregation of the membrane protein corresponds to the ion release time, indicating that the longer the ion release time, the greater the damage to the membrane protein.

  The above results are explained by the following mechanism. In other words, the bacteria applied to the agar medium are initially exposed on the surface of the agar medium alone, the cell membrane is destroyed by contact with the ions in the air, and the intracellular protein flows out. It is possible. Membrane dysfunction due to the outflow of this protein is thought to lead to inactivation (sterilization) of bacteria. In FIG. 9 shown in Experimental Example 1, it is considered that the result of the above-described action is shown.

<Experimental example 5>
Next, it was demonstrated that the ions used in the above-described experiments have no DNA damaging power and no carcinogenic action. In Experimental Example 5, air containing ions was released into Enterococcus malodoratus and Bacillus (Bacillus: Bacillus subtilis) in the same manner as in Experimental Example 1, unreleased, 1 hour after release, and 2 hours after release. DNA extracted from each of the bacteria at the time point by a conventional method was electrophoresed. FIG. 17 is a photograph showing the results of Experimental Example 5. In FIG. 17, each lane means the following.

-EK: Enterococcus ion not released-E1: Enterococcus ion released for 1 hour-E2: Enterococcus ion released for 2 hours-BK: Bacillus ion not released-B1: Bacillus ion released for 1 hour-B2: Bacillus ion 2 Time Release In the two lanes in the center of FIG. 17, as a control experiment, the results of fragmentation of each of the DNA extracts from Enterococcus and Bacillus causing a standard positive reaction are shown.

As a result, the DNA appeared as a single band even after the release of ions, and thus it became clear that the DNA was not single-stranded. That is, even when ions are released, DNA is not damaged and there is no risk of carcinogenicity. In this test, H ₃ O (H ₂ O) _n (n is 0 or a natural number) as positive ions and O ₂ (H ₂ O) _m (m is 0 or a natural number) as negative ions are mainly released. However, the reactive particles generated by the discharge are not limited to the above substances. The same effect can be expected even if the above two or more kinds of substances, for example, ions or radicals such as N ₂ ⁺ , O ₂ ⁺ , NO ₂ ⁻ and CO ₂ ⁻ are included.

  As described above, when the air containing ions is released to the bacterium, the cell membrane of the bacterium is destroyed, but the result that the internal DNA is preserved is explained as follows. The substances that contribute to the above protein destruction are positive ions and negative ions released into the space. According to our experiments, the lifetime in the space is about 5 to 30 seconds, depending on conditions. This is because ions are reactive particles and collide with dust and ions in the air and react to disappear. Therefore, when this ion comes into contact with the cell, the reaction with the cell proceeds rapidly, but it is considered that the internal DNA is not affected due to its short lifetime. The effects as described above are considered to be realizable if the particle lifetime is the same as or shorter than that of ions. For example, the same effect is exhibited even with OH radicals having a lifetime in air of about 1 μsec. It is thought that.

Consider the necessary concentration of the reactive particles for protein destruction. Since cells are considered to be approximately 10 μm in size, one million cells are lined up in an area of 1 cm ² . In order to destroy these proteins, it is necessary to contact particles comparable to the amount of cells. Therefore, the number of particles having reactivity is several times, for example, about 5 million particles in 1 cm ^3. If there is a number, a certain proteolytic efficiency can be ensured. In addition, for airborne fungi in the air, for example, when there is one fungus per 1 cm ^{3 in} space, the number of reactive particles is as long as the collision probability is 1/1000. If there are about 1,000 particles in 1 cm ³ , a certain level of proteolytic efficiency of floating bacteria can be secured.

  From the above, it is possible to destroy the cell membrane and preserve the DNA by using particles having a relatively short lifetime.

<Experimental example 6>
Next, it is known that bacteria have a self-repairing ability, and when the sterilization treatment is insufficient (for example, when the ultraviolet irradiation time is short or the dose of a drug is small), the bacteria are revived and proliferate. Therefore, an irreversibility test of bacterial inactivation by positive and negative ions was performed. As the bacterial species, Enterococcus malodoratus, Staphylococcus chromogenes, Micrococcus roseus, and Sarcina flava were used.

  Bacteria were attached on the agar medium, and air containing both positive and negative ions was released for 90 minutes in the same manner as in Experimental Example 1. The bacteria after ion treatment were stored at 4 ° C. for 3 days at low temperature. This gave the bacteria recovery time. The time-dependent change in the number of remaining bacteria due to ion release was measured when stored at low temperature and when not stored. FIG. 18 is a graph showing the results for Enterococcus marododoratus, FIG. 19 is the results for Staphylococcus chromogenes, FIG. 20 is the results for Micrococcus roseus, and FIG. There was no significant difference in time-dependent change due to the presence or absence of cryopreservation in all four types of bacteria, and no recovery of bacteria due to cryopreservation was observed.

  In addition, bacteria were attached on the agar medium, and air containing positive and negative ions was released for 90 minutes in the same manner as in Experimental Example 1, and then cultured in an incubator at 37 ° C. for 48 hours to generate bacterial colonies. Thereafter, the cells were further cultured for 21 days at 37 ° C., and an experiment for examining the presence of new colonies was also conducted. As a result, generation of new colonies was not confirmed even after culturing for 21 days. Bacterial recovery was not observed even in the growth environment.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is defined by the terms of the claims, rather than the description above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.

  Furthermore, in order to examine the deterioration effect of the medium due to the release of ions, the bacteria were adhered on the agar medium, and then air containing both positive and negative ions was released. Thereafter, the bacteria were transferred to a medium that did not release ions, and the recovery of the bacteria was examined, but no recovery of the bacteria was observed.

  From these, it can be seen that the bacteria inactivation method by releasing air containing reactive particles eliminates the self-repairing ability of bacteria and completely kills them.

  Although the treatment method of the present invention is effective for attached objects, it can be expected to have an effect on bacteria, viruses, and proteins floating in the space. Therefore, both the attached substances and the suspended substances have the reactivity. You may make it discharge | release the air containing particle | grains.

It is a figure which shows typically 1st Embodiment of the processing method of this invention. It is a figure which shows typically 2nd Embodiment of the processing method of this invention. It is a flowchart which shows the process process of 3rd Embodiment of the processing method of this invention. It is a figure which shows typically 4th Embodiment of the processing method of this invention. It is a figure which simplifies and shows the discharge means 31 used suitably for the processing apparatus of this invention. It is a figure which shows typically the processing apparatus 41 of a preferable example of this invention. It is a figure which shows notionally the processing apparatus 51 of the other example of this invention. It is a figure which shows typically the treatment apparatus 61 used for the evaluation test. 6 is a graph showing the results of Experimental Example 1. It is a graph which shows the result about Penicillium chrysogenum of Experimental example 2 (Penicillium chrysogenum). It is a graph which shows the result about Stachybotrys chartram (Experiment 2). It is a graph which shows the result about the Aspergillus versicocolor of Experimental example 2 (Aspergillus versicolor). It is a graph which shows the result about the Penicillium camamberti of Experimental example 2 (Penicillium cambertii). It is a graph which shows the result about the cladosporium herbarum (Experimental Example 2). It is a photograph which shows the result about the Aspergillus versicolor and the cladosporium herbarum of Experimental example 3 (Cladosporium herbarum). 6 is a photograph showing the results of Experimental Example 4. 10 is a photograph showing the results of Experimental Example 5. It is a graph which shows the result about the enterococcus malodolatus of Experimental example 6 (Enterococcus malodoratus). It is a graph which shows the result about the staphylococcus chromogenes (Staphylococcus chromogenes) of Experimental example 6. It is a graph which shows the result about the micrococcus rose of Experimental example 6 (Micrococcus roseus). It is a graph which shows the result about the saltina flava (Sarcina flava) of Experimental example 6.

  DESCRIPTION OF SYMBOLS 1 Kamaboko, 2 Membrane, 3 Spatula, 4 Air duct, 6 Protective layer, 11 Tableware, 12 Frame, 13 Remaining foodstuff, 14 Cleaning liquid, 15 Air containing reactive particles, 21 Ham mass, 22 Liquid or film-like material, 23 radical discharge port, 24 radical path, 25 bag, 31 discharge means, 32 dielectric, 33 discharge electrode, 34 counter electrode, 35 power supply, 41 processing device, 42 housing, 43 container, 44 Liquid, 45 Stirrer, 51 Processing device, 52 Discharge means, 53 Input means, 54 Storage means, 55 Calculation means, 56 Control means.

Claims (1)

Means for generating positive ions H ₃ O ⁺ (H ₂ O) _n (n is 0 or a natural number) and negative ions O ₂ ⁻ (H ₂ O) _m (m is 0 or a natural number) as reactive particles ; ,
Means for releasing the reactive particles;
An input means for inputting the name of the microorganism, protein or virus that is the inactivation target;
Storage means for storing biochemical feature data relating to the inactivated object in advance;
A calculation unit that collates data stored in the storage unit with information input by the input unit and predicts the inactivation performance of the inactivation target;
A control means for controlling the release means so as to release the reactive particles to the inactivation target under a condition that fragments the protein of the inactivation target based on the result of the calculation means. Equipment .

Images