JP4327035B2 - Optical tweezers - Google Patents

Description

  The present invention relates to an optical tweezer device that controls the position of a minute object by the radiation pressure of light.

  Optical tweezers that control the position of minute objects with the radiation pressure of light are various, such as non-contact manipulation and cell fusion of living cells in the fields of biology and medicine, or array of fine particles and rotational driving of minute objects in the mechanical field. The range of application is expanding in the field, and further development is expected.

The configuration of a conventional optical tweezer device is shown in FIG.
As shown in FIG. 19, a conventional optical tweezer device includes a light source 191, a spatial mode filter including a lens 192, a pinhole 193, and a lens 194, a mirror 195 for switching an optical path, a high NA lens 196, a stage. 197.
A sample 198 is placed on the stage 197.

With this configuration, when the light from the light source 191 is rapidly narrowed down and applied to the sample 198, a force toward the focal position of the light acts on the sample 198 due to the radiation pressure of the light.
For example, if the sample 198 is a living body and the wavelength of the light source 191 is set to a wavelength with less absorption by the living body, the position of the sample 198 can be moved in a non-contact manner, and the position of the sample 198 can be controlled using this. It is possible to evaluate mechanical characteristics.

Such optical tweezers are already on the market and are increasingly used (see Non-Patent Document 1).
"Series: Life Science Opened by Light" Vol.7: New Photonics Technology that Opens Life Science "Japan Photobiology Association Editor-in-Chief: Takashi Funatsu Kyoritsu Shuppan 1999/12

However, the conventional optical tweezers have the following problems.
First, the conventional optical tweezers cannot be used in places where there are mechanical vibrations due to the complexity of the optical system, and the price is high.

  Second, when trying to control a plurality of samples at the same time with the conventional optical tweezers, it is necessary to prepare a plurality of light sources, and the optical system becomes complicated.

  Third, the conventional optical tweezers device requires a mechanical movable part for moving the stage, and has a problem that the moving speed is limited to the mechanical speed of the stage.

  In order to solve the above problems, an optical tweezer device according to claim 1 of the present invention includes a control light generation source and a planar optical waveguide circuit, and the planar optical waveguide circuit collects light at a focal point. An optical means and a sample insertion groove formed in a direction perpendicular to the planar optical waveguide circuit, wherein the sample insertion groove is provided along a condensing position by the waveguide type condensing means, Further, a condensing position variable mechanism for moving the condensing position along the sample insertion groove is provided. With such a configuration, an inexpensive optical tweezer device that does not require a complicated optical system can be provided.

  According to a second aspect of the present invention, in addition to the configuration of the first aspect, the optical tweezer device includes a phase modulator array in which the waveguide type condensing means includes the condensing position varying mechanism, and the phase modulation. And a slab optical waveguide optically connected to the vessel array. With such a configuration, it is possible to provide an optical tweezer device that does not require a mechanically movable portion.

  According to a third aspect of the present invention, in addition to the configuration of the first aspect, the optical tweezer device includes: an optical waveguide for input; a first slab optical waveguide; A plurality of channel optical waveguides optically connected to the slab optical waveguide and a second slab optical waveguide optically connected to the plurality of channel optical waveguides. Even with such a configuration, it is possible to provide an optical tweezer device that does not require a mechanical movable portion.

  According to a fourth aspect of the present invention, in addition to the configuration of the third aspect, in addition to the configuration of the third aspect, the plurality of channel optical waveguides are sequentially numbered from one end to 1, 2, and N (N is an integer of 2 or more). When numbered, the optical length of the n-th channel optical waveguide satisfies L0 + nΔL (L0 and ΔL are both positive real numbers and n is an integer of 1 to N). With such a configuration, it is possible to provide an optical tweezers device that can control the condensing position by wavelength or temperature.

An optical tweezers device according to a fifth aspect of the present invention includes, in addition to the configuration of the third aspect, the plurality of channel optical waveguides in order from one end, 1, 2, and N (N is an integer of 2 or more). When the numbers are assigned, the optical length of the nth channel optical waveguide is L _o 0 + nΔL _o / 2 (L _o 0, ΔL _o are both positive real numbers, and n is 1 or more and N or less when n is an even number. met even), n is L _e 0+ when the odd _{(n + 1) ΔL e /} 2 (L e 0, ΔL e are both positive real numbers, n represents meets the following odd) 1 or more n, L _o 0 and L _e 0 is different. With such a configuration, it is possible to make the light collection profile both, and it is possible to trap a sample having a refractive index lower than that of the medium.

Further, the optical tweezer device according to claim 6 of the present invention, in addition to the arrangement of claim 5, wherein the [Delta] L _o and [Delta] L _e are equal. With such a configuration, it is possible to provide an optical tweezer device in which the trap characteristic does not change even when the wavelength is changed.

  The optical tweezer device according to claim 7 of the present invention is characterized in that, in addition to the configurations of claims 3 to 6, the converging position varying mechanism is realized by temperature adjustment of the planar optical waveguide circuit. . With such a configuration, it is possible to provide an optical tweezer device that does not require any mechanical movable part.

  According to an eighth aspect of the present invention, in addition to the configurations of the third to seventh aspects, the control light generation source includes a wavelength variable means for realizing the condensing position variable mechanism. And By setting it as such a structure, the optical tweezers apparatus which can change a trap position with a wavelength can be provided.

  According to a ninth aspect of the present invention, in addition to the configuration of the third to eighth aspects, the optical tweezer device includes a plurality of light sources having different wavelengths by the control light generation source and a multiplexing unit that multiplexes the plurality of light sources. Means are provided. With such a configuration, it is possible to provide an optical tweezer device capable of independently controlling a plurality of samples simultaneously.

  The optical tweezer device according to claim 10 of the present invention is characterized in that, in addition to the configuration of claim 3, the optical lengths of the plurality of channel optical waveguides are all set equal.

  According to an eleventh aspect of the present invention, in addition to the configurations of the third to tenth aspects, the plurality of channel optical waveguides include a phase variable adjusting means. By adopting such a configuration, the sample position can be adjusted regardless of the mechanical movable part.

  An optical tweezer device according to a twelfth aspect of the present invention, in addition to the configurations of the third to eleventh aspects, is a light that bifurcates light between the input optical waveguide and the first slab optical waveguide. A branching means is provided. By adopting such a configuration, it is possible to make the light collection profile both, and it is possible to trap a sample having a refractive index lower than that of the medium.

  According to a thirteenth aspect of the present invention, in addition to the configuration of the first to twelfth aspects, the planar optical waveguide circuit is a silica glass optical waveguide formed on a silicon substrate or a quartz substrate. It is characterized by. By adopting such a configuration, an optical tweezer device having excellent reliability can be provided.

  The optical tweezers according to claim 14 of the present invention is characterized in that, in addition to the configuration of claim 11, the phase variable adjustment means is a thin film heater. By adopting such a configuration, an optical tweezer device having excellent reliability can be provided.

  According to a fifteenth aspect of the present invention, in addition to the structure of the first aspect, the sample insertion groove has a plurality of sample chambers.

  By using the optical tweezers of the present invention, it is possible to provide an optical tweezers that is inexpensive and simple and does not require mechanically movable parts.

The following embodiment is mentioned as the best form for implementing this invention.
In the present embodiment, parts having the same function are denoted by the same reference numerals, and redundant description thereof is omitted.
Furthermore, in the following embodiments, the planar optical waveguide circuit is a silica glass optical waveguide formed on a silicon substrate.

This is because such a combination can provide an optical waveguide that is stable and excellent in workability.
However, the present invention is not limited to this combination. Of course, other substrates and glass films may be used.

The structure of the optical tweezers according to the first embodiment of the present invention is shown in FIG.
The present embodiment relates to claims 1, 2, and 13.
That is, as shown in FIG. 1, the optical tweezer device according to the present embodiment is optically connected to the control light generation source 11, the optical transmission path 12 that guides light from the control light generation source 11, and the optical transmission path 12. The planar optical waveguide circuit 13 includes a waveguide type condensing means 14 and a sample insertion groove 15.
Here, an optical fiber can be used as the optical transmission line 12.

However, the present invention is not limited to this example, and other optical transmission lines such as a flexible optical waveguide may be used.
As the control light source 11, light having a wavelength of 1.06 microns from the G laser to Nd: Y can be used. This is because, when this configuration is used, the sample can be trapped efficiently with little absorption by the living body or water.
However, the present invention is not limited to this example, and other configurations such as an optical fiber laser may be used as the light source.
Of course, other wavelengths such as 1.55 microns may be used.

FIG. 2 shows the configuration of the planar optical waveguide circuit 13 of the optical tweezer device according to the present embodiment.
A cylindrical lens 21 is provided at the input portion of the planar optical waveguide circuit 13, whereby light is condensed in the vertical direction and input to the planar optical waveguide 13.
The spread light is spatially modulated by the phase modulator array 22, passes through the slab optical waveguide 23, and is condensed in the vicinity of the sample insertion groove 24 formed in the vertical direction in the planar optical waveguide circuit 13.
The sample insertion groove 24 contains a sample 25 together with a solvent.

Here, in the planar optical waveguide circuit 13 of the optical tweezer device according to the present embodiment in FIG. 2, the cylindrical lens 21 is used as the input unit. This is because this configuration can provide a simple means for condensing light only in the vertical direction.
However, the present invention is not limited to this example, and it goes without saying that such a cylindrical lens 21 may not be used.

The phase modulator array 22 can be realized by a liquid crystal spatial modulator, for example.
However, the present invention is not limited to this example, and can of course be realized by another method such as providing a thin film heater on the planar optical waveguide circuit 13.
Next, the operation of the optical tweezer device according to the first embodiment of the present invention will be described.
The light output from the control light generation source 11 is condensed in the vertical direction by the cylindrical lens 21.
As a result, the sample 25 is attracted to the condensing position and trapped.

Here, the spatial light profile at the phase modulator array 22 and the spatial light profile at the condensing position are in a Fourier transform relationship.
Therefore, when a spatially linear phase shift is given to the phase modulator array 22, the light profile at the condensing position moves in parallel.

That is, the position where the sample 25 is trapped can be adjusted only by the phase change applied to the phase modulator array 22.
As described above, when the optical tweezer device according to the first embodiment of the present invention is used, the position of the sample trapped by the light can be adjusted without a mechanically movable part, so that a robust optical tweezer device can be provided. .

The structure of the optical tweezers according to the second embodiment of the present invention is shown in FIG.
The present embodiment relates to claims 1, 3, 4, 8, and 13.
That is, as shown in FIG. 3, the optical tweezer device according to the present embodiment uses a wavelength tunable laser 31 as a control light generation source, and the planar optical waveguide circuit 13 includes an input optical waveguide 32 and a first optical waveguide 32. One slab optical waveguide 33, a plurality of channel optical waveguides 34, a second slab optical waveguide 35, and a sample insertion groove 24 are provided.

Further, the optical length of the plurality of channel optical waveguides 34 is such that the length Ln of the nth channel optical waveguide is Ln when numbered 1, 2,..., N from the innermost channel optical waveguide. = L0 + nΔL was set.
Here, L0 and ΔL are positive real numbers.

A cross-sectional view of the sample insertion groove 24 of the optical tweezers according to the present embodiment is shown in FIG.
As shown in FIG. 4, the clad 42 formed on the silicon substrate 41 is provided with a sample insertion groove 24 so as to cross the second slab optical waveguide 35.
A sample 25 is prepared in the sample insertion groove 24 so that the sample 25 floats on the water 26.

Here, in the cross-sectional view of the sample insertion groove 24 of the optical tweezer device according to the present embodiment in FIG. 4, the sample 25 floats on the water 26, but the present invention is not limited to this example, and the solvent Can be anything.
Next, the operation of the optical tweezer device according to the second embodiment of the present invention will be described with reference to the drawings.

In the optical tweezer device according to the second embodiment of the present invention shown in FIG. 3, the control light output from the wavelength tunable laser 31 is spread by the first slab optical waveguide 33 through the input optical waveguide 32.
The expanded optical field is carried by the channel optical waveguide 34 and reaches the second slab optical waveguide 35.
The optical field is narrowed by the second slab optical waveguide 35 and condensed near the sample insertion groove 24.

Now, the condensing position at this time is translated by the inclination of the phase (wavefront) at the entrance of the second slab optical waveguide 35.
Since the inclination of the phase varies depending on the wavelength, if the oscillation wavelength of the wavelength tunable laser 31 is changed, the condensing position is translated.

FIG. 5 shows a state of the single particle trap in the optical tweezers according to the second embodiment of the present invention. Here, the refractive index of the sample 25 which is a particle is assumed to be higher than the refractive index of the water 26.
At this time, when the oscillation wavelength of the wavelength tunable laser 31 is changed as described above, the trap position can be changed.

FIG. 6 shows a configuration of an optical tweezer device according to a modification of the second embodiment of the present invention.
The modification of the present embodiment relates to claims 1, 3, 4, 8, 9, and 13.
That is, as shown in FIG. 6, in addition to the configuration of the optical tweezers according to the second embodiment of the present invention, the light from the two variable wavelength light sources 31a and 31b and the light from the two variable wavelength light sources 31a and 31b are combined. A wavelength division multiplexing optical coupler 61 is provided.

Here, in FIG. 6, the number of variable wavelength light sources 31 is two, but this is for the sake of simplicity of explanation, and the number of variable wavelength light sources 31 may be three, four, or any other number. Is not limited to this example.
In FIG. 6, the wavelength multiplexing optical coupler 61 is used to multiplex the light from the two variable wavelength light sources 31a and 31b. This is because this configuration can provide a multiplexing means with little loss. is there.

However, the present invention is not limited to this example, and a wavelength-independent optical coupler may be used.
FIG. 7 shows the state of two particles and wrapping in the optical tweezer device according to the modification of the second embodiment of the present invention.
Here, the refractive index of the sample 25 which is a particle is assumed to be higher than the refractive index of the water 26.
As in the case of the single particle trap in the optical tweezers device according to the second embodiment of the present invention shown in FIG. 5, each trap position can be changed depending on the wavelength, so that the positions of the two particles can be controlled independently. .

Such characteristics are important, for example, when two materials are joined.
FIG. 8 shows a state of a single particle trap in the optical tweezers according to the modification of the second embodiment of the present invention.
Here, the refractive index of the sample 25 which is a particle is assumed to be lower than the refractive index of the solvent 81.

Thus, when the refractive index of the sample 25 is lower than the surrounding refractive index, the sample is trapped where the light intensity is low.
As shown in FIG. 6, in the optical tweezer device according to the modification of the second embodiment of the present invention, the two condensing points can be freely controlled, and thus the low-refractive-index sample 25 can be trapped in this way. It becomes possible.

FIG. 9 shows a configuration of an optical tweezer device according to a second modification of the second embodiment of the present invention.
The modification of the present embodiment relates to claims 1, 3, 4, 8, and 13.

That is, as shown in FIG. 9, in addition to the configuration of the optical tweezer device according to the second embodiment of the present invention, the sample insertion groove 24 has a plurality of sample chambers 91.
The sample insertion groove 24 may not have a uniform width and may have such a complicated shape.
When the sample chamber 91 is provided in this way, the sample 25 can be trapped and separated into the sample chamber 91.

The structure of the optical tweezers according to the third embodiment of the present invention is shown in FIG.
The present embodiment relates to claims 1, 3, 4, 7, and 13.
That is, as shown in FIG. 10, the optical tweezer device according to the present embodiment uses a narrow linewidth laser 101 as a control light generation source, and the planar optical waveguide circuit 13 includes an input optical waveguide 32, A first slab optical waveguide 33, a plurality of channel optical waveguides 34, a second slab optical waveguide 35, and a sample insertion groove 24 are provided.

Furthermore, a temperature control means 102 is provided below the planar optical waveguide circuit 13.
Further, the optical length of the plurality of channel optical waveguides 34 is such that the length Ln of the nth channel optical waveguide is Ln when numbered 1, 2,..., N from the innermost channel optical waveguide. = L0 + nΔL was set.
Here, L0 and ΔL are positive real numbers.

Here, in the optical tweezers device according to the third embodiment of the present invention shown in FIG. 10, the temperature control means 102 is provided at the lower part of the planar optical waveguide circuit 13. I do not care.
Now, the condensing position at this time is translated by the inclination of the phase (wavefront) at the entrance of the second slab optical waveguide 35.
If the temperature of the planar optical waveguide circuit 13 is changed by the temperature control means 102, the optical length of the plurality of channel optical waveguides 34 changes due to the thermo-optic effect, so that the phase gradient changes and the condensing position moves in parallel. It will be.

FIG. 11 shows a state of one particle trap in the optical tweezers according to the third embodiment of the present invention.
Here, the refractive index of the sample 25 which is a particle is assumed to be higher than the refractive index of the water 26.
At this time, if the temperature of the planar optical waveguide circuit 13 is changed by the temperature control means 102 as described above, the trap position can be changed.

FIG. 12 shows the configuration of an optical tweezer device according to a fourth embodiment of the present invention.
The present embodiment relates to claims 1, 3, 5, 8, and 13.
That is, as shown in FIG. 12, the optical tweezers device according to the present embodiment uses a variable wavelength laser 31 as a control light generation source, and the planar optical waveguide circuit 13 includes an input optical waveguide 32, a first optical waveguide, One slab optical waveguide 33, a plurality of channel optical waveguides 34, a second slab optical waveguide 35, and a sample insertion groove 24 are provided.

Furthermore, a temperature control means 102 is provided below the planar optical waveguide circuit 13.
The optical length of the arrayed waveguide of the optical tweezer device according to the fourth embodiment is shown in FIG.
As shown in FIG. 13, the optical lengths of the plurality of channel optical waveguides 34 are those of the nth channel optical waveguide when numbered 1, 2,... N from the innermost channel optical waveguide. length Ln is, when n is an even number L _o 0 + nΔL _o / 2
When n is an odd number, L _e 0+ (n + 1) ΔL _e / 2
It is set to satisfy. _{Here, L o 0, ΔL o,} L e 0, ΔL e are both positive real number.

In the optical tweezer device according to a fourth embodiment of the present invention, [Delta] L _o and [Delta] L _e was equal.
This is because this configuration can provide an optical tweezer device in which the distance between the two focused spots does not change when the oscillation wavelength of the variable wavelength light source 31 is changed.
However, the present invention is not limited to this example. Of course, ΔL _o and ΔL _e may be different.

FIG. 14 shows a state of the single particle trap of the optical tweezers according to the fourth embodiment of the present invention.
As described with reference to FIG. 13, in the optical tweezer device according to the fourth embodiment of the present invention, the even-numbered and odd-numbered channel optical waveguides create different wavefronts. Produces two focused spots.
Therefore, if the refractive index of the sample 25 is lower than the refractive index of the solvent 81, the sample can be trapped so as to be sandwiched between the two focused spots.

The structure of the optical tweezers according to the fifth embodiment of the present invention is shown in FIG.
The present embodiment relates to claims 1, 3, 4, 8, 12, and 13.
That is, as shown in FIG. 15, the optical tweezers according to the present embodiment uses a wavelength tunable laser 31 as a control light generation source, and the planar optical waveguide circuit 13 includes an input optical waveguide 32, an optical waveguide, A branch circuit 151, a first slab optical waveguide 33, a plurality of channel optical waveguides 34, a second slab optical waveguide 35, and a sample insertion groove 24 are provided.

Here, in the optical tweezers according to the fifth embodiment of the present invention, the optical branch circuit 151 is a Y branch optical circuit. This is because this configuration can provide an optical branch circuit with less wavelength dependency.
However, the present invention is not limited to this example. Of course, the optical branch circuit may be realized by using other means such as a multimode interference optical coupler and a directional coupler.

Further, the optical length of the plurality of channel optical waveguides 34 is such that the length Ln of the nth channel optical waveguide is Ln when numbered 1, 2,..., N from the innermost channel optical waveguide. = L0 + nΔL
It was set to satisfy. Here, L0 and ΔL are positive real numbers.

With this setting, an optical field having two spots is generated by the optical branching circuit 151 on the input side of the first slab optical waveguide 33.
The spatial distribution of these two spots is guided by a plurality of channel optical waveguides 34 and copied to the output end of the second slab optical waveguide 35.
Therefore, two condensing spots can be realized with this configuration.
FIG. 16 shows a state of the single particle trap in the optical tweezers according to the fifth embodiment of the present invention.

Here, the refractive index of the sample 25 which is a particle is assumed to be lower than the refractive index of the solvent 81.
At this time, as described above, two focused spots are generated at the output end of the second slab optical waveguide 35, so that the sample 25 can be trapped.
Further, when the oscillation wavelength of the wavelength tunable laser 31 is changed, the trap position can be changed.

The configuration of the optical tweezers according to the sixth embodiment of the present invention is shown in FIG.
The present embodiment relates to claims 1, 3, 10, 11, 13, and 14.
That is, as shown in FIG. 17, the optical tweezer device according to the present embodiment uses a narrow linewidth laser 101 as a control light generation source, and the planar optical waveguide circuit 13 includes an input optical waveguide 32, A first slab optical waveguide 33, a plurality of channel optical waveguides 34, a second slab optical waveguide 35, and a sample insertion groove 24 are provided.

Each of the plurality of channel optical waveguides 34 includes a phase modulator array 171.
Here, in the optical tweezers device according to the sixth embodiment of the present invention shown in FIG. 17, the plurality of channel optical waveguides 34 are provided with a thin film heater as the phase modulator array 171 on the planar optical waveguide 13 and are phased by the thermo-optic effect. I did modulation. This is because this configuration can provide an optical tweezer device with excellent reliability.

However, the present invention is not limited to this example, and it is needless to say that a phase modulator array using another principle such as an electro-optic effect may be used.
In the optical tweezer device according to the sixth embodiment of the present invention shown in FIG. 17, the lengths of the plurality of channel optical waveguides 34 are equal to each other. This is because this configuration can provide an optical tweezer device with little wavelength dependency.

However, the present invention is not limited to this example, and the lengths of the plurality of channel optical waveguides 34 may of course be different from each other.
As described above, when the plurality of channel optical waveguides 34 are provided with the phase modulator array 171, the wavefront of light on the entrance side of the second slab optical waveguide 32 can be freely controlled. The spot shape and the number of spots on the exit side can be controlled.

FIG. 18 shows a state of one particle trap in the optical tweezers according to the sixth embodiment of the present invention.
Here, the refractive index of the sample 25 which is a particle is assumed to be higher than the refractive index of the water 26.
At this time, as described above, since the position of the focused spot can be controlled by the control state of the phase modulator array 171 of the plurality of channel optical waveguides 34, the sample 25 can be trapped and the trapped position can be changed. it can.
Note that the optical tweezer device according to the sixth embodiment of the present invention shown in FIG. 17 can generate not only the spot position but also a plurality of spots. Sample traps with a lower refractive index than such solvents are also possible.

As described above, the present invention relates to an optical tweezers device that controls the position of a minute object by the radiation pressure of light, and a focusing means on a planar optical waveguide circuit and a sample along the focusing position. There is a characteristic in the structure which formed the groove | channel for insertion.
The position of the sample (object) can be controlled by varying this condensing means, and an optical tweezer device having various functions such as eliminating the need for a mechanical movable part by using a slab type optical waveguide, etc. Realized.

  The optical tweezers of the present invention can be widely used in various fields such as non-contact manipulation and cell fusion of living cells in the fields of biology and medicine, or in the mechanical field such as arrangement of microparticles and rotational driving of minute objects. .

1 is a schematic configuration diagram illustrating an optical tweezers device according to a first embodiment of the present invention. It is a schematic block diagram which shows the planar optical waveguide of the optical tweezers apparatus which concerns on 1st embodiment of this invention. It is a schematic block diagram which shows the optical tweezers apparatus which concerns on 2nd embodiment of this invention. It is sectional drawing of the planar optical waveguide of the optical tweezers device concerning 2nd embodiment of this invention. It is explanatory drawing showing the example of 1 particle trap in the optical tweezers which concern on 2nd embodiment of this invention. It is a schematic block diagram which shows the optical tweezers of the deformation | transformation of 2nd embodiment of this invention. It is explanatory drawing showing the example of the two-particle trap in the optical tweezers of the deformation | transformation of 2nd embodiment of this invention. It is explanatory drawing showing the example of 1 particle | grain trap in the optical tweezers of the deformation | transformation of 2nd embodiment of this invention. It is a block diagram of the groove | channel for sample insertion in the optical tweezers apparatus of the 2nd deformation | transformation of 2nd embodiment of this invention. It is a schematic block diagram which shows the optical tweezers apparatus which concerns on 3rd embodiment of this invention. It is explanatory drawing showing the example of 1 particle trap in the optical tweezers which concern on 3rd embodiment of this invention. It is a schematic block diagram which shows the optical tweezers apparatus which concerns on 4th embodiment of this invention. It is a graph showing the optical length of the arrayed waveguide of the optical tweezers device concerning a fourth embodiment of the present invention. It is explanatory drawing showing the example of 1 particle | grain trap in the optical tweezers which concern on 4th embodiment. It is a schematic block diagram which shows the optical tweezers which concern on 5th embodiment of this invention. It is explanatory drawing showing the example of 1 particle | grain trap in the optical tweezers which concern on 5th embodiment of this invention. It is a schematic block diagram which shows the optical tweezers apparatus which concerns on 6th embodiment of this invention. It is explanatory drawing showing the example of 1 particle | grain trap in the optical tweezers apparatus which concerns on 6th embodiment of this invention. It is a schematic block diagram which shows the conventional optical tweezers.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Control light generation source 12 Optical transmission path 13 Planar optical waveguide circuit 14 Waveguide type condensing means 15 Sample insertion groove 21 Cylindrical lens 22 Phase modulator array 23 Slab optical waveguide 24 Sample insertion groove 25 Sample 31 Variable wavelength laser 32 Input optical waveguide 33 First slab optical waveguide 34 Channel optical waveguide 35 Second slab optical waveguide 41 Silicon substrate 42 Clad 61 Wavelength multiplexing optical coupler 81 Solvent 91 Sample chamber 101 Narrow linewidth laser 102 Temperature control means 151 Optical branch circuit 171 Phase modulator array 191 Light source 192 Lens 193 Pinhole 194 Lens 195 Mirror 196 High NA lens 197 Stage 198 Sample

Claims (15)

A control light generation source and a planar light waveguide circuit, the planar light waveguide circuit being a waveguide type condensing means for collecting light at a focal point, and a sample insertion formed in a direction perpendicular to the planar light waveguide circuit The sample insertion groove is provided along the light collection position by the waveguide type light collection means, and further, the light collection position variable mechanism for moving the light collection position along the sample insertion groove An optical tweezer device characterized by comprising: 2. The optical tweezer device according to claim 1, wherein said waveguide type condensing means includes a phase modulator array having said condensing position variable mechanism, and a slab optical waveguide optically connected to said phase modulator array An optical tweezers device comprising: 2. The optical tweezer device according to claim 1, wherein the waveguide type condensing means includes an input optical waveguide, a first slab optical waveguide, and a plurality of optically connected to the first slab optical waveguide. And an optical tweezers device comprising: a second slab optical waveguide optically connected to the plurality of channel optical waveguides. 4. The optical tweezer device according to claim 3, wherein when the plurality of channel optical waveguides are numbered 1, 2,..., N (N is an integer of 2 or more) in order from one end, the nth channel optical waveguide. Of the optical tweezers satisfying L0 + nΔL (L0 and ΔL are both positive real numbers and n is an integer of 1 to N). 4. The optical tweezer device according to claim 3, wherein when the plurality of channel optical waveguides are numbered 1, 2,..., N (N is an integer of 2 or more) in order from one end, the nth channel optical waveguide. When n is an even number, it satisfies L _o 0 + nΔL _o / 2 (L _o 0, ΔL _o are both positive real numbers, n is an even number between 1 and N), and n is an odd number sometimes _{L e 0+ (n + 1)} △ L e / 2 (L e 0, △ L e are both positive real numbers, n represents the following odd one or more n) satisfies the, characterized in that L _o 0 and L _e 0 is different Optical tweezers. An optical tweezer device according to claim 5, △ L _o and △ L _e optical tweezers and wherein the equal. 7. The optical tweezers device according to claim 3, 4, 5, or 6, wherein the condensing position varying mechanism is realized by adjusting a temperature of the planar optical waveguide circuit. 8. The optical tweezers device according to claim 3, 4, 5, 6, or 7, wherein the control light generation source has wavelength variable means for realizing the condensing position variable mechanism. 9. The optical tweezer device according to claim 3, 4, 5, 6, 7, or 8, wherein the control light generation source includes a plurality of light sources having different wavelengths and a multiplexing unit that multiplexes the plurality of light sources. An optical tweezer device characterized by. 4. The optical tweezer device according to claim 3, wherein the optical lengths of the plurality of channel optical waveguides are all set equal. 11. The optical tweezers device according to claim 3, 4, 5, 6, 7, 8, 9 or 10, wherein the plurality of channel optical waveguides are provided with phase variable adjustment means. 12. The optical tweezer device according to claim 3, 4, 5, 6, 7, 8, 9, 10 or 11, wherein light is bifurcated between the input optical waveguide and the first slab optical waveguide. An optical tweezer device comprising optical branching means for performing 13. The optical tweezer device according to claim 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12, wherein the planar optical waveguide circuit is formed on a silicon substrate or a quartz substrate. An optical tweezer device characterized by being a silica-based glass optical waveguide. 12. The optical tweezer device according to claim 11, wherein the phase variable adjusting means is a thin film heater. 2. The optical tweezers device according to claim 1, wherein the sample insertion groove has a plurality of sample chambers.

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