JP4295632B2 - Occult blood test equipment - Google Patents

Description

  The present invention relates to a occult blood detection device used to detect a minute amount of blood (occult blood) that cannot be detected with the naked eye. The present invention relates to a occult blood test apparatus that is preferably used for predicting the blood pressure and is safe for the human body.

  A sanitary napkin or large panty liner for absorbing menstrual bleeding is configured to absorb a certain amount or more of blood. Therefore, these sanitary napkins and relatively large panty liners are bulky and give the user a sense of discomfort. For this reason, it is desirable that the attachment is performed only when bleeding actually occurs.

  However, in order to install a sanitary napkin or a relatively large panty liner only at the time when the bleeding actually occurs, it is necessary to predict the bleeding start time by some method. However, there was no method for reliably predicting the onset of bleeding. Therefore, in the past, the bleeding start time was estimated from each individual's menstrual cycle, and a sanitary napkin or a large panty liner was prophylactically attached several days before the estimated bleeding start time to prepare for bleeding.

  Therefore, sanitary napkins and large panty liners for absorbing blood must be worn from the time when they are not originally needed, and the user feels uncomfortable from the time when wearing before the start of bleeding is not necessary. Restricted their behavior. In addition, unnecessary mounting work is necessary, which has caused a great deal of inconvenience to the user.

  As a method for detecting blood, dimethylbenzidine, tetramethylbenzidine or tolidine is added to the blood as a chromogen, and peroxide hydrogen peroxide cumene, hydrogen peroxide or dimethyldihydroperoxyhexane is added. A color reaction that causes the above to act is known. However, the drugs used for this reaction are not only irritating to the human body, but are also suspected to be carcinogenic and are harmful. Therefore, it is not appropriate to detect blood using such a drug in such a way that it directly contacts the body in the above-mentioned use.

  On the other hand, a guaiac test method is known as a relatively safe blood detection method (for example, Non-Patent Document 1 below). The guaiac test method using guaiac fat used as a food additive as a coloring reagent is particularly used in the field of forensic medicine. However, it was necessary to add turpentine oil to guaiac oil for color development. Guayac fat is used as a food additive and has no problem in safety. However, turpentine oil used for color development has skin irritation. Therefore, it was not appropriate to use the combination of guaiac oil and turpentine oil directly on the human body.

However, in the following Patent Document 1, a disposable sanitary article for women is disclosed in which the surface of a disposable panty liner is coated with such a coloring drug and an oxidizing agent by spraying or the like. Here, It is said that trace blood can be detected. In this prior art, cyclodextrin and nitrone are added to prevent the above-described deterioration of the reaction over time. However, the coloring drugs and oxidizing agents used have irritation to the human body and still have a problem in safety.
WO 00/65084 Chemical Dictionary, Volume 3, Page 7 (Kyoritsu Shuppan Co., Ltd. Fermentation), Reduced Edition 32nd Edition (issued August 15, 1989)

  As described above, the advent of hygiene products such as women's panty liners that are safe for the human body, exhibit sufficient color to detect trace amounts of blood, and have excellent temporal stability of the color reaction is desired. There was no such product yet.

  The object of the present invention is, in view of the current state of the art, is safe for the human body, can reliably detect a minute amount of blood with the naked eye, and is excellent in the temporal stability of the detectability of the minute amount of blood. Another object is to provide a occult blood test apparatus and a panty liner that is the occult blood test apparatus.

  The occult blood test apparatus according to the present invention comprises a first reagent made of guaiac fat and a second reagent made of edible oil, and the first and second reagents and the sample are brought into contact with each other during use to cause color development due to occult blood. The resulting occult blood detection agent is provided.

  In a specific aspect of the occult blood test apparatus according to the present invention, a guaiac fat-attached sheet to which a first reagent made of guaiac fat is attached and an edible oil-attached sheet to which a second reagent made of edible oil is attached. Are stacked.

  In another specific aspect of the occult blood test apparatus according to the present invention, a water-absorbing film is laminated between the guaiac fat adhesion sheet and the edible oil adhesion sheet.

  In still another specific aspect of the occult blood test apparatus according to the present invention, the guaiac fat-attached sheet and the edible oil-attached sheet are bonded together using an adhesive.

  In still another specific aspect of the present invention, a panty liner is provided as the occult blood test apparatus.

  In the occult blood test apparatus according to the present invention, the occult blood detection agent comprises a first reagent made of guaiac fat and a second reagent made of edible oil, and the first and second reagents and the sample are contacted during use. Color development due to occult blood occurs. Therefore, guaiac fat with excellent safety is used as the first reagent, and edible oil with excellent safety is also used as the second reagent for coloring guaiac fat. Unlike turpentine oil, edible oil has excellent safety for the human body and other organisms. Therefore, the occult blood test apparatus according to the present invention is excellent in safety and can be directly applied to the human body.

  In addition, the color reaction due to guaiac fat occurs rapidly by the addition of edible oil. Therefore, occult blood, which is a trace amount of blood, can be detected easily and quickly.

  Therefore, the occult blood test apparatus according to the present invention is excellent in safety and is preferably used for prompt detection of occult blood, which is a very small amount of blood.

  In addition, the first and second reagents are brought into contact with the sample during use. In other words, the first and second reagents are separated until use. Therefore, the guaiac fat and the edible oil are kept stable until use, so that the occult blood detection performance hardly deteriorates over time.

In particular, in the case where a guaiac fat-attached sheet to which a first reagent made of guaiac fat is attached and an edible oil-attached sheet to which a second reagent made of edible oil is attached are made of guaiac fat. After the first reagent is brought into contact with the sample, the edible oil as the second reagent is brought into contact, or the edible oil as the second reagent is brought into contact with the sample and then brought into contact with the first reagent. Accordingly, guaiac fat and edible oil are each kept more stable until use. Therefore, deterioration of the occult blood detection performance over time is less likely to occur.

  When a water-absorbing film is disposed between the guaiac fat-adhered sheet and the edible oil-adhered sheet, the water released from the human body during use is retained in the water-absorbing film, and the water is present during bleeding. It is used as a solvent in the color reaction. Therefore, even though the occult blood detection agent is a non-solution system, the color development reaction can be reliably started.

  Moreover, when the guaiac fat adhesion sheet and the edible oil adhesion sheet are bonded together with an adhesive, the guaiac fat adhesion sheet and the edible oil adhesion sheet are physically and stably fixed. Accordingly, for example, when a panty liner is attached, a shift between the guaiac fat-attached sheet and the edible oil-attached sheet is unlikely to occur.

  In addition, when the adhesive has the property of retaining moisture, the moisture released from the human body is retained in the adhesive as in the case of using the water-absorbing film, and the moisture is present at the time of bleeding. It is used as a solvent in the color reaction. Therefore, even though the occult blood detection agent is a non-solution system, the color development reaction can be reliably started.

  Since the panty liner according to the present invention is configured as the occult blood test apparatus, according to the present invention, it is possible to provide a panty liner that is safe for the human body and that can reliably detect a small amount of blood. .

  Details of the present invention will be described below.

(Guayac fat)
The guaiac fat used as the first reagent in the present invention is a substance used for detecting blood together with turpentine oil in the guaiac test in the forensic field. Guayac fat is also widely used in food additives as an antioxidant. Guayac fat is a natural resin and contains α-guaiaconic acid, β-guaiaconic acid, guaiaretic acid, and the like as components. The guaiac fat used in the present invention is not particularly limited, but those that are safe for the human body, such as food additive-qualified products, are preferably used.

  In the present invention, guaiac fat is used as an expression including the above components contained in guaiac fat.

  Although the specific form in which the said guaiac fat is used is not specifically limited, Preferably, it is used with the form of the guaiac fat adhesion sheet | seat in which the guaiac fat was adhered to the sheet | seat base material. Such a guaiac fat adhering sheet can be constituted, for example, by adhering guaiac fat to a sheet substrate made of a water permeable sheet such as a woven fabric or a non-woven fabric such as a panty liner or a piece of filter paper.

  When impregnating a sheet base material such as a woven fabric, a non-woven fabric or a filter paper with guaiac fat, the sheet base material may be impregnated with a solution obtained by dissolving or suspending guaiac fat in a solvent and dried.

The solvent is not particularly limited, and an appropriate liquid that can dissolve or suspend guaiac fat and can be easily removed during drying is used. Considering the safety to the human body when remaining, ethyl alcohol or the like is preferably used as the solvent.

The guaiac fat is dissolved or suspended in a solvent, and after removing the solvent, 1 to 500 μg / cm ² , more preferably 5 to ² per unit area of the sheet base material used for the guaiac fat-attached sheet.
It is desirable to be used so as to have an amount of 00 μg / cm ² . If the amount of guaiac fat impregnated is less than the above range, the color intensity may be insufficient. If it is too large, water is repelled and the reaction takes time if the sample is hydrophilic. Sometimes.

  In addition, guaiac fat does not develop color when left alone, and guaiac fat does not develop color even when mixed with edible oil described below, and when coexisted with erythrocytes, particularly hemoglobin, Colors blue and occult blood is visualized.

(Cooking oil)
The edible oil used as the second reagent in the present invention is not particularly limited. Examples include vegetable oils such as jojoba oil, rosehip oil, corn oil, sunflower oil, coconut oil, rapeseed oil and mint oil. In particular, suitable safe vegetable oils used for food such as sesame oil, safflower oil, cottonseed oil, olive oil, orange oil or soybean oil are preferably used.

  In the present invention, “edible oil” is an expression included in edible oil and includes components that develop guaiac fat when coexisting with blood, such as fatty acids and esters thereof.

  The edible oil may be attached to guaiac fat and the same part such as a woven fabric, a nonwoven fabric or a filter paper piece constituting a panty liner when used. Preferably, the edible oil and the guaiac fat are separately provided so that the edible oil and the guaiac fat are mixed when the sample containing occult blood penetrates.

  Although the specific form in which the said edible oil is used is not specifically limited, Preferably, it is used with the form of the edible oil adhesion sheet | seat with which edible oil is adhered to the sheet | seat base material. Such an edible oil adhering sheet can be constituted, for example, by adhering edible oil to a sheet substrate made of a water permeable sheet such as a woven fabric or a non-woven fabric constituting a panty liner or the like and a filter paper piece.

  There is no particular limitation on the form and structure of contacting the sample, guaiac fat, and edible oil as long as guaiac fat develops color and can reveal occult blood by contacting the blood component with guaiac fat and edible oil during use. .

  In addition, the edible oil may be used as it is, or may be dissolved or suspended in a solvent as necessary and impregnated or coated on a necessary part. Further, if necessary, the solvent may be dried and attached to a necessary portion. When a solvent is used, the solvent is not particularly limited, and an appropriate liquid that can dissolve or suspend edible oil and can be easily removed during drying can be used. As such a liquid, ethyl alcohol or ethyl acetate is preferably used in consideration of safety to the human body when remaining.

Moreover, when the said edible oil adhesion sheet is used, 0.1-80 mg / cm < ² > per sheet base-unit unit area which comprises this edible oil adhesion sheet, More preferably, 0.2-
It is desirable that the edible oil adheres in the range of 60 mg / cm ² . If the amount of edible oil attached is less than the above range, the color intensity may be insufficient. If it is too much, the edible oil will be repelled if the sample, i.e. the sample is hydrophilic, and it will take a long time to react. There is.

  In addition, as an edible oil, you may use the fatty acid and fatty acid ester which are the components of an edible oil in this invention.

  The fatty acid esters are not particularly limited, and examples include fatty acid esters of fatty acids and alcohols containing polyhydric alcohols. Examples of such fatty acid esters include fatty acid esters that are safe for the human body, such as isopropyl myristate, butyl myristate, ethyl linoleate, and isopropyl palmitate. As for the said edible oil and fatty acid ester, only 1 type may be used and multiple types may be used.

  In addition, the application amount per unit area of a guaiac fat adhesion sheet | seat and an edible oil adhesion sheet | seat is set as the application amount per unit area of a guaiac oil or an edible oil application part, when apply | coating intermittently in stripe form or dot shape.

(Occult blood test device)
In the occult blood test apparatus according to the present invention, occult blood is detected using the first reagent made of guaiac fat and the second reagent made of edible oil. In this case, as described above, the first reagent and the second reagent may be brought into contact with the sample, that is, the specimen by application or the like at the time of use. However, when considering the temporal stability of the reactivity of the reagent, convenience, etc., the form in which the first reagent comprising guaiac fat is brought into contact with the sample and then mixed with the second reagent, or A form in which the second reagent and the sample are brought into contact with each other and then mixed with the first reagent is preferable.

  In the occult blood test apparatus according to the present invention, preferably, a guaiac fat-attached sheet to which the first reagent is attached and an edible oil-attached sheet to which the second reagent is attached are laminated. In this case, the guaiac fat-attached sheet to which guaiac fat is attached and the edible oil-attached sheet to which edible oil is attached are prepared and laminated as separate sheets. Is more stable. On the other hand, when a small amount of blood comes into contact with guaiac fat and edible oil, strong color development occurs. Therefore, the stability of the guaiac oil and edible oil until use is maintained, so that the occult blood detection capability of the occult blood test apparatus is unlikely to deteriorate over time, and an extremely small amount of bleeding can be reliably detected during use. it can.

(Sheet base material)
In the present invention, the sheet base material for constituting the guaiac fat-attached sheet and the edible oil-attached sheet is not particularly limited as long as it is permeable to moisture as described above. Moreover, the sheet base material for constituting the guaiac fat-attached sheet and the edible oil-attached sheet may be composed of a single sheet or a laminate in which a plurality of sheets are laminated. Accordingly, as the sheet base material, a woven fabric, a nonwoven fabric or a laminate thereof having no water repellency is appropriately used.

  Moreover, in the structure by which the said guaiac fat adhesion sheet | seat and the cooking oil adhesion sheet | seat were laminated | stacked, any sheet | seat may be used for the side which contacts a human body. However, in the guaiac fat-attached sheet or vegetable oil-attached sheet used on the side that comes into contact with the human body, when blood penetrates, the guaiac oil or edible oil moves with the blood to the next layer, so that color development is performed quickly. It is desirable that the thickness is 400 μm or less or mesh.

In addition, when a guaiac fat adhesion sheet or an edible oil adhesion sheet is used as a sheet | seat arrange | positioned on the outer side which is the opposite side to the human body, the thickness of a sheet base material is not specifically limited. This is because the sheet base material used on the side in contact with the human body needs to be promptly sent to the next layer after the adhering guaiac fat or edible oil is mixed with the absorbed moisture and sample. On the other hand, the edible oil adhesion sheet or the guaiac fat adhesion sheet used on the outer side opposite to the side in contact with the human body does not require such a function.

  In the laminated sheet in which the guaiac fat-attached sheet and the edible oil-attached sheet are laminated, the edible oil-attached sheet side is preferably used so as to be in contact with the human body. By arranging the edible oil adhesion sheet on the side in contact with the human body, the safety of the occult blood test apparatus can be further enhanced.

  Preferably, a liquid-impermeable film made of a synthetic resin or the like is further laminated on the surface opposite to the side in contact with the human body of the occult blood test apparatus of the present invention, that is, the outer surface of the outer sheet. By laminating such a liquid impermeable film, blood leakage to the outside can be suppressed. More preferably, a moisture-absorbing material layer such as a moisture-absorbing woven fabric, non-woven fabric, cotton or resin material may be disposed between the outer surface of the outer sheet and the liquid-impermeable film. Thereby, blood leaked to the outer surface of the guaiac fat or edible oil adhesion sheet can be absorbed.

  In addition, when laminating | stacking a guaiac fat adhesion sheet | seat and an edible oil adhesion sheet | seat, it is desirable to pressurize in the state which laminated | stacked the guaiac fat adhesion sheet | seat and the edible oil adhesion sheet | seat, and to integrate so that both sheets may not shift | deviate.

  In the present invention, preferably, a water-absorbing film is laminated between the guaiac fat-attached sheet and the edible oil-attached sheet. The material constituting the water-absorbing film is not particularly limited as long as the material has water absorption. However, it is preferable that the retention and release of moisture is performed digitally, that is, the moisture is continuously absorbed and retained until the amount of moisture that can be retained is exceeded. Those having the property of dissolving and functioning as a reaction solvent are preferably used. When a film having such characteristics is applied to a human body that constantly evaporates moisture, moisture evaporates as well as moisture is absorbed. The absorption and transpiration of water are balanced in a state where the film maintains a water content below a threshold value. Therefore, the film itself dissolves rapidly due to stimulation such as microbleeding, assists the reaction as a solvent, and can effectively cause a coloring reaction.

  Specifically, a film made of a water-soluble polymer such as starch, pullulan, polyvinyl alcohol, polyvinyl pyrrolidone, hydroxypropyl starch, hydroxypropylcellulose, or the like is used as the water-absorbing film.

  Although the thickness of a water absorptive film is not specifically limited, Preferably it is 5-60 micrometers, More preferably, it is 10-40 micrometers. If the water-absorbing film is too thin, the strength and oxidant barrier property may be insufficient, and if it is too thick, it may not be easily dissolved during bleeding and the coloring intensity may be insufficient.

  In the present invention, the guaiac fat-attached sheet and the edible oil-attached sheet may be bonded together with an adhesive. Although it does not specifically limit about the adhesive agent used, While adhering both sheets, what can hold | maintain the water | moisture content from a human body is used preferably.

  Such adhesives include, for example, starch paste, chemical synthesis such as pullulan, polyvinyl alcohol, etc., water and organic solvents such as polyvinylpyrrolidone, hydroxypropyl starch, hydroxypropyl cellulose, etc. An appropriate hygroscopic adhesive capable of adhering the sheet is used.

The adhesive serves to adhere the guaiac fat-attached sheet and the edible oil-attached sheet to maintain the structure of the occult blood test apparatus and to prevent direct contact between the guaiac fat and the edible oil. In addition, the hygroscopic adhesive dissolves at the time of bleeding and also functions to form a color reaction field.

  In addition, since the guaiac grease-attached sheet and the edible oil-attached sheet are bonded with an adhesive, there is no gap between both sheets, and both reagents can be mixed easily and quickly, and the color development is more reliable. Become.

  About the solvent which melt | dissolves the said adhesive agent, if it volatilizes by drying and solidifies an adhesive agent, without modifying an adhesive agent, it will not specifically limit. Even if it remains, a solvent such as water or ethanol that is safe for the human body is preferably used.

The amount of the adhesive used is not particularly limited, but is preferably 0.3 to 100 g / m ² , more preferably 1 to 80 g / m ² in terms of weight as a solid content after drying. If the amount of the adhesive used is small, the adhesive strength is lowered, and it may be damaged due to delamination or the barrier property by the adhesive may be insufficient. When the amount of the adhesive used is too large, the sheet becomes hard, the feeling of use is deteriorated, it is difficult to easily dissolve or swell in water at the time of bleeding, and the coloring intensity may be insufficient.

  In order to adjust the fluidity of the adhesive solution and the affinity to the sheet, a thickener, surfactant, etc. may be added to the above adhesive, and the physical properties of the adhesive after drying are adjusted. In order to do so, a softener or the like may be mixed. These other components and materials used arbitrarily may be appropriately selected depending on the feeling of use and processability, and are not particularly limited. However, it is desirable to use components and materials that are safe for humans and do not interfere with the color development reaction.

  Moreover, when bonding a guaiac fat adhesion sheet | seat and an edible oil adhesion sheet | seat, the said adhesive agent does not need to be a solution state, You may use the film adhesive which shows adhesiveness. Furthermore, a cross-linking agent containing a polyvalent metal ion such as aluminum may be added to the adhesive as necessary in order to increase the strength of the occult blood test apparatus.

(Other)
In the occult blood test apparatus according to the present invention, a surfactant, a water-absorbing polymer material, or the like may be further used in order to enhance the color development reaction. Moreover, in order to ensure the structure and appearance as a laminated sheet or panty liner, a binder, a colorant, a softening agent, or the like may be used. These other optionally used components and materials may be appropriately selected depending on the feeling of use and processing suitability, and are not particularly limited, but those that are safe for the human body and do not interfere with the coloring reaction may be used as appropriate. .

  In either case, occult blood is visualized by the reaction of guaiac oil with erythrocytes in the blood, particularly hemoglobin, and further by edible oil or fatty acid ester contained in edible oil, and the presence or absence of occult blood is easy to visually check. Therefore, it can be detected reliably and reliably.

  In the present invention, the mixing ratio of guaiac fat and edible oil is not particularly limited, and even if both are used in a wide range, occult blood can be developed and occult blood can be visually confirmed. However, in order to obtain sufficient coloring intensity, it is desirable that the ratio of guaiac fat is 50% by weight or less with respect to the total of guaiac fat and edible oil.

[Examples and Comparative Examples]
The invention will now be elucidated by describing non-limiting examples of the invention.

(1) Raw material (a) Guayac fat: manufactured by Kanto Chemical Co., Inc., reagent-standard guaiac fat (b) Edible oil The following various edible oils were prepared as edible oils.

Olive oil (Release: Hanshin Pharmacopoeia, Standard: Nippon Paint Pharmacopoeia)
Sesame oil (release: Wako Pure Chemicals, standard: for food)
Safflower oil (Release: Wako Pure Chemicals, Standard: For food)
Cottonseed oil (Release: Wako Pure Chemicals, Standard: For food)
Orange oil (Release: Wako Pure Chemical, Standard: Wako Grade 1)
Soybean oil (Release: Wako Pure Chemical, Standard: Wako Grade 1)
Isopropyl myristate (Release: Nikko Chemicals, Standard: Supplementary Drugs)
Butyl myristate (Release: Nikko Chemicals, Standard: Makeup Coordination)
Ethyl linoleate (Release: Nikko Chemicals, Standard: Cosmetic Regulation)
Isopropyl palmitate (release: Nikko Chemicals, Standard: Kabara)
Butyl myristate (Release: Nikko Chemicals, Standard: Makeup Coordination)

In addition, the meaning of the code | symbol of the said medicine supplement regulations, makeup arrangement regulations, and makeup original regulations is as follows.
Medicinal product regulations: Pharmaceutical additive standards, Cosmetic composition regulations: Cosmetic ingredient standard, Cosmetic standards: Cosmetic raw material standards (c) Turpentine oil (Release: Wako Pure Chemicals, Standard: Reagents)
(D) Surfactant: Polyoxyethylene sorbitan monooleate ( 20E.O.), (Release: Nikko Chemicals, Standard: Make-up Base)
(E) Sheet base material: made of cotton, 30 g / m ² in weight, about 70 μm in thickness
(F) Water-absorbing film: potato starch film (oblate, manufactured by Yodogawa Oblate Co., Ltd., about 20 μm)
(G) Water absorbent material ... absorbent cotton (Japanese Pharmacopoeia)
(H) Liquid impervious film: Polyethylene film having a thickness of about 30 μm (i) Water-soluble adhesive: Polyvinyl alcohol adhesive (trade name: Arabic)

[Preparation of Bleeding Detection Laminated Sheets of Examples 1-27]
(1) Production of guaiac fat-attached sheet The sheet base material is impregnated with an ethanol solution of guaiac fat at a concentration of 0.05 w / v% so that the dry weight of guaiac fat is 30 μg / cm ^2. And dried at room temperature to obtain a guaiac fat-attached sheet. The weight of guaiac fat deposited per unit area was calculated from the difference in weight before and after the sheet was processed.

(2) Preparation of edible oil adhesion sheet Using the same sheet base material that was used to prepare the guaiac oil adhesion sheet, the edible oil ethyl acetate solution (concentration 20% W / V%) was the dry weight of the edible oil. As a result, it was impregnated so as to be about 2 mg / cm ^2, and then dried at room temperature to obtain an edible oil adhesion sheet. The weight of edible oil adhered per unit area was calculated from the difference in weight before and after the sheet was processed.

(3) Production of occult blood test device a) occult blood test device (two-layer type)
The edible oil adhesion sheet prepared as described above, the guaiac fat adhesion sheet, the water absorbing material and the polyethylene film as the liquid impermeable film are laminated in this order, and pressed with a punching die having a size of 5 × 15 cm, Punched out. When the cross section of the obtained laminate was observed, it was confirmed that the layers were compressed and integrated. In this manner, a two-layer occult blood test apparatus in which the edible oil adhesion sheet and the guaiac fat adhesion sheet were directly laminated was obtained. The two-layer type two layers mean that the edible oil adhesion sheet and the guaiac fat adhesion sheet as the constitution of the present invention are laminated, and the water absorbing material and the polyethylene film are not included in the number of layers.

b) Occult blood test device (3-layer type)
On the other hand, the edible oil adhesion sheet prepared as described above, the water absorbent film, the guaiac fat adhesion sheet, the water absorbent material and the polyethylene film are laminated in this order, and pressed with a punching die having a size of 5 × 15 cm, A three-layer occult blood test apparatus was obtained by punching. Here, when the cross section of the laminate was observed, it was confirmed that it was compressed and the layers were integrated. The three-layer type three layers mean three layers of a vegetable oil adhesion sheet, a water-absorbing film, and a guaiac fat adhesion sheet.

c) Blood test device (1 body type)
The edible oil adhesion sheet | seat was bonded together and integrated with the guaiac fat adhesion sheet prepared as mentioned above using a water-soluble adhesive. The weight of the water-soluble adhesive per unit area was 10 g / m ² . In addition, this water-soluble adhesive weight was calculated | required by calculating from the weight difference before and behind the process of an adhesion sheet.

[Production of occult blood test apparatuses of Comparative Examples 1 to 3]
(1) Raw material The same material as the raw material used in the Example was prepared.

(2) Production of turpentine oil-attached sheet In the edible oil-attached sheet produced in Example 1, turpentine oil was used instead of edible oil, and an ethyl acetate solution (concentration 20% W / V%) of turpentine oil was used as the dry weight of turpentine oil. The sheet base material was impregnated so as to be about 2 mg / cm ^2, and then dried at room temperature to obtain a turpentine oil-adhered sheet.

  (3) Comparative example 1 [occult blood test apparatus (two-layer type)], comparative example 2 [occult blood test apparatus (three-layer type)] using the above turpentine oil-adhered sheet according to the method for preparing the laminated sheet of the example, And each occult blood test apparatus of comparative example 3 [occult blood test apparatus (one body type)] was produced.

[Evaluation of Examples 1-27 and Comparative Examples 1-3]
About the occult blood test apparatuses of Examples 1 to 27 and Comparative Examples 1 to 3 prepared as described above, 1) color development intensity, 2) skin irritation, 3) stability, 4) usability, And 5) The color development time was evaluated.

1) Evaluation of color intensity 20 μL of heparin-added rabbit blood diluted to 1/1000 by volume with physiological saline was dropped on the edible oil or turpentine oil-attached sheet surface of each occult blood test apparatus of Examples and Comparative Examples. The color intensity was evaluated.

2) Evaluation of skin irritation The occult blood test apparatus of the example or comparative example punched to 1 × 1 cm on the back of a shaved rabbit was applied with the edible oil or turpentine oil adhesion sheet as the skin side, covered with gauze from above, Fixed with adhesive bandage. The sheet was removed after 24 hours, and the redness of the skin surface on the back of the rabbit was observed.

3) Evaluation of stability Each occult blood test apparatus of Examples and Comparative Examples was sealed with an aluminum laminate film and stored at a temperature of 60 ° C. for 2 weeks, and then the color intensity of 1) was evaluated. Compared.

4) Evaluation of usefulness Four volunteers used each of the occult blood test apparatuses of the examples and comparisons, and calculated the average time of this time by calculating the time when micro-bleeding could be detected before full-scale bleeding. . In addition, about the comparative example, since the inferiority with respect to the Example was clear by other evaluation, and irritation was recognized, the usefulness was not evaluated.

5) Color development time The color development time indicates the time until the color change is stabilized.

  The results are shown in Tables 1 to 3.

表１〜表３の比較から明らかなように、吸水性フィルムをグアヤク脂付着シートと食用油付着シートの間に配置した３層型の潜血検査装置及び上記一体型の潜血検査装置では、２層型の潜血検査装置に比べて、経時による安定性が高められることがわかる。

As is clear from the comparison of Tables 1 to 3, the three-layer occult blood test apparatus in which the water-absorbing film is arranged between the guaiac fat-attached sheet and the edible oil-attached sheet and the integrated occult blood test apparatus have two layers. It can be seen that the stability over time is improved compared to the type of occult blood test apparatus.

[Examples 28 to 35]
Each occult blood test apparatus of Examples 28-35 was produced and evaluated in the same manner as in Example 23 except that the amounts of guaiac fat and olive oil were changed as shown in the following table in Example 23. . The results are shown in Table 4.

表４から明らかなように、グアヤク脂の量を１〜５００μｇ／ｃｍ²の範囲とし、その
場合にオリーブ油の量を０．２〜８０ｍｇ／ｃｍ²の範囲とすることにより、発色強度を
効果的に高め得ることがわかる。また、グアヤク脂の量を１〜２００μｇ／ｃｍ²とし、
かつオリーブ油の量を０．１〜６０ｍｇ／ｃｍ²とした場合には、発色時間を十分短くし
得ることがわかる。より好ましくは、グアヤク脂の量を１〜２００μｇ／ｃｍ²とし、オ
リーブ油の量を０．２〜６０ｍｇ／ｃｍ²とした場合には、発色強度が十分であり、かつ
発色時間を効果的に短くし得ることがわかる。

As is clear from Table 4, the amount of guaiac fat is in the range of 1 to 500 μg / cm ² , and in this case, the amount of olive oil is in the range of 0.2 to 80 mg / cm ² , so that the coloring intensity is effective. It can be seen that it can be increased. The amount of guaiac fat is 1 to 200 μg / cm ² ,
In addition, when the amount of olive oil is 0.1 to 60 mg / cm ² , it can be seen that the color development time can be sufficiently shortened. More preferably, when the amount of guaiac fat is 1 to 200 μg / cm ² and the amount of olive oil is 0.2 to 60 mg / cm ² , the coloring intensity is sufficient and the coloring time is effectively shortened. You can see that

[Examples 36 to 38]
Further, each of the guaiac oil adhesion sheet and the edible oil adhesion sheet used in Examples 23, 26, and 27 was further added with an ethyl acetate solution (concentration: 10% W / V%) of polyoxyethylene sorbitan monooleate ( 20E.O.). ) Was impregnated to a dry weight of about 1 mg / cm ^2, and then each occult blood test apparatus of Examples 36 to 38 was constructed using those dried at room temperature, and evaluated in the same manner as in Example 23. did. The results are shown in Table 5.

表５から明らかなように、グアヤク脂付着シート及び食用油付着シートのそれぞれに、さらにモノオレイン酸ポリオキシエチレンソルビタン（２０Ｅ．Ｏ．）を付着させた場合には、発色時間をより一層短縮し得ることがわかる。そして、その場合においても、発色強度が十分であり、かつ皮膚刺激性も認められなかった。

As is clear from Table 5, when polyoxyethylene sorbitan monooleate ( 20E.O.) was further adhered to each of the guaiac oil-adhered sheet and the edible oil-adhered sheet, the color development time was further shortened. You can see that Even in this case, the color intensity was sufficient and skin irritation was not observed.

  In addition, the meaning of the symbol of the evaluation result of Table 1-Table 5 is as follows.

Color intensity: A: Clear blue, B: Blue, B: Light blue, X: No change Skin irritation: As shown in Table 6 below.

安定性…◎：製造直後と差がないこと、○：製造直後よりも弱いが判定可能であること、×：判定不能であることをそれぞれ示す。

Stability: A: Indicates that there is no difference from immediately after manufacture, B: Indicates that it is weaker than that immediately after manufacture but can be determined, and X: Cannot be determined.

  Usefulness: Time from detection time to full-scale bleeding (for example, 20 means detection was made 20 hours before full-scale bleeding.-: Not implemented)

Claims (4)

A first reagent composed of guaiac fat and a second reagent composed of edible oil are provided, and a occult blood detection agent that causes color development due to occult blood by contacting the first and second reagents and the sample during use is provided. ,And,
And guaiac attached sheet first reagent consisting of guaiac is attached, you characterized in that the second reagent is deposited edible oil adhering sheet made of edible oil have been stacked, occult blood test apparatus. The guaiac and fat deposition sheet, wherein the water-absorbing film is laminated between the edible oil adhered sheet, occult blood test apparatus according to claim 1. The occult blood test apparatus according to claim 1 , wherein the guaiac fat adhesion sheet and the edible oil adhesion sheet are bonded together using an adhesive. The occult blood test apparatus according to any one of claims 1 to 3 , wherein the occult blood test apparatus is a panty liner.