JP4237084B2 - Target detection substrate, target detection apparatus, and target detection method - Google Patents

Description

  The present invention relates to a target detection apparatus and a target detection method that can detect various targets such as pathogenic substances, biological substances, and toxic substances efficiently, reliably, simply, and with good sensitivity even under high temperature conditions.

Conventionally, various methods for detecting various targets such as pathogenic substances, biological substances, and toxic substances have been studied. For example, enzyme immunoassay methods such as ELISA are known. However, in these, there existed problems, such as having to use an expensive fluorescent label, a dangerous radiation marker, etc.
Recently, there have been proposed devices and methods for detecting various targets by detecting interference color change of interference light by a detection layer for detecting the various targets without using fluorescent labels or radiation markers. Yes. For example, there has been proposed an apparatus for measuring an unspecific protein layer thickness change as an interference color change using an ellipsometer or the like (see Patent Documents 1 and 2). In addition, it has been proposed to detect a change in thickness on a light reflecting surface due to a nucleic acid chain as an interference color change (see, for example, Patent Documents 3 to 5). In addition, an apparatus has been proposed in which light emitted from a light source is irradiated onto a sample surface via a polarizer, the reflected light is reflected by a polarization modulator, and detected via the polarizer (for example, Patent Documents 6 to 6). 9). There has also been proposed an apparatus for measuring a change in film thickness of a non-specific protein layer with an ellipsometer or the like as an interference color change of interference light and detecting the interference light via a polarizer (see, for example, Patent Document 10). .

  However, in these cases, as a result of interaction occurring only on the surface of the non-specific protein layer (target interaction layer), the reaction area with the target is reduced, and simple and rapid measurement cannot be performed, and measurement noise is reduced. There are problems that it is easy to pick up, measurement error is large, and measurement sensitivity is not sufficient. In addition, in the interaction between the target and the target interaction layer, the film thickness of the target interaction layer itself does not change, and therefore the increase in film thickness after the interaction depends on the size of the target. Therefore, there is a problem that by controlling the change in film thickness to an arbitrary film thickness, measurement errors are suppressed and it is difficult to improve measurement sensitivity. In addition, since the target interaction layer is inferior in heat resistance, there is a problem that it cannot be used again once it is placed under a high temperature. Furthermore, there is also a problem that it cannot be quantified. Therefore, by controlling the change in the film thickness so as to have an arbitrary film thickness, the various targets such as pathogenic substances, biological substances, and toxic substances can be obtained under high temperature conditions without using an expensive measuring device. However, the present situation is that a target detection apparatus and a target detection method that can be detected easily, quickly, and with extremely high sensitivity and efficiency with a small measurement error have not yet been provided. ing.

JP 2002-122603 A JP 2002-116208 A Japanese Patent Publication No. 7-32720 JP-A-10-288616 JP 2001-235473 A JP 61-34442 A JP-A-4-78122 Japanese Examined Patent Publication No. 62-57936 U.S. Pat. No. 4,332,476 JP 2002-122603 A

  An object of the present invention is to solve the conventional problems in response to the above-mentioned demands and achieve the following object. That is, the present invention can detect various targets such as pathogenic substances, biological substances, toxic substances, etc. easily and quickly with a small measurement error, without using an expensive measuring device, and with high sensitivity and efficiency. It is another object of the present invention to provide a target detection apparatus and a target detection method capable of quantitative determination, and a target detection base material suitably used for them.

Means for solving the problems are as follows. That is,
<1> irradiated emit light as interference light, to the wavelength of the interference light before interacting with the target, the target interacts with the interference light wavelength changeable target detecting group after the Material,
Target detection substrate, and at least have a can interact target interaction layer and target, and,
The thickness of the target interaction layer after the target detection substrate contacts with the liquid and interacts with the target is larger than the thickness of the target interaction layer before contact with the liquid Is a target detection substrate. The in target detection substrate according to <1> emits the irradiation light and the interference light, the relative wavelength of the interference light before that interacts with a target, after interacting with the target the wavelength of the interference light is a target detection substrate for possible changes, target detection substrate, and at least have a can interact target interaction layer and target, and, the target detection substrate The thickness of the target interaction layer after contacting with the liquid and interacting with the target is greater than the thickness of the target interaction layer before contacting with the liquid. For this reason, the target interaction layer comes into contact with the liquid material and interacts with the target, thereby increasing the thickness of the target interaction layer itself, and even after the liquid material is removed, the target interaction layer Shrinkage of the thickness in the working layer is suppressed. As a result, the target is suitable for various detections in which the target is detected easily and reliably with a small measurement error, and quickly and with extremely high sensitivity.

<2> The target interaction layer includes a filamentous body having a plurality of target interaction portions capable of interacting with the target, and the filamentous body is erected in contact with the liquid material. The target detection substrate according to <1>, wherein the target interaction part interacts with the target. In the target detection substrate according to <2>, the filamentous body is erected by contacting the liquid material. In this state, the target interacts with the target interaction part, and thus the target interaction part that is present inside the target interaction layer and does not appear on the surface before contacting with the liquid material. However, after contact with the liquid material, it becomes possible to interact with the target. Further, the target interacts with the target interaction portion, so that the shrinkage of the thickness in the target interaction layer is suppressed even after the liquid material is removed. As a result, it is suitable for various detections in which the target is detected simply and reliably with a small measurement error, and quickly and with extremely high sensitivity.
<3> The thickness (X) of the target interaction layer after the target interaction layer comes into contact with the liquid and interacts with the target, and the thickness (X) of the target interaction layer before contact with the liquid ( Y) is the target detection substrate according to any one of <1> to <2>, which satisfies a relationship of the following formula: [(XY) / Y] × 100> 50%. In the target detection substrate according to <3>, the thickness (X) and the thickness (Y) satisfy the relationship of the following formula [(XY) / Y] × 100> 50%. It is suitable for various detections in which the target is detected easily, reliably, and quickly with a very high sensitivity with a small measurement error.

  <4> The target detection substrate according to any one of <1> to <3>, wherein one end of the filament is bonded to a surface of a layer adjacent to the target interaction layer. In the target detection substrate according to <4>, one end of the filamentous material is bonded to the surface of a layer adjacent to the target interaction layer. Thereby, before contacting the liquid material, the filamentous material is laid down while supporting the one end of the filamentous material, and after contacting the liquid material, the one end of the filamentous material is supported. It is erected as it is. As a result, when the target interaction layer comes into contact with the liquid material, the thickness of the target interaction layer itself increases, and when the target interacts with the target interaction layer, the liquid material is Even after removal, the shrinkage of the thickness in the target interaction layer is suppressed. As a result, it is suitable for various detections in which the target is detected easily and reliably with a small measurement error, and quickly and with extremely high sensitivity.

<5> The target detection substrate according to <4>, wherein the adhesion is a chemical bond. In the target detection substrate according to <5>, after the adhesion is a chemical bond, one end of the filamentous body is firmly adhered, and the target interacts with the target interaction layer Also, the detachment of the filamentous body is suppressed. As a result, it is suitable for various detections in which the target is detected simply and reliably with a small measurement error, and quickly and with high sensitivity.
<6> The target detection substrate according to any one of <2> to <5>, wherein the filament is a filamentous organic molecule.
<7> The target detection substrate according to <6>, wherein the filamentous organic molecule is at least one of a linear polymer and a helical polymer.
<8> The above-described <6>, wherein the filamentous organic molecule has at least one monomer unit selected from an imine group-containing compound, an amine group-containing compound, a hydroxyl group-containing compound, an amide group-containing compound, and a carboxyl group-containing compound. To <7>.
<9> The target detection substrate according to any one of <6> to <8>, wherein the filamentous organic molecule is a helical polymer.

<10> The target detection substrate according to <9>, wherein the helical polymer is an α-helix polypeptide.
<11> The target detection base material according to any one of <6> to <10>, wherein the polymerization degree of the filamentous organic molecule is 20 to 10,000. In the target detection substrate according to <11>, since the degree of polymerization is in a predetermined range, the target interaction layer is in contact with the liquid material and after interacting with the target, The thickness of the target interaction layer is within a predetermined range. As a result, it is suitable for various detections in which the target is detected easily, reliably, quickly, and with extremely high sensitivity with a small target error.
<12> The target detection base material according to any one of <6> to <11>, wherein the number average molecular weight (Mn) of the filamentous organic molecule is 1,000 to 1,000,000. In the target detection base material according to <12>, since the number average molecular weight is in a predetermined range, the target interaction layer in the previous period comes into contact with the liquid and interacts with the target. The thickness of the target interaction is within a predetermined range. As a result, it is suitable for various detections in which the target is detected simply and reliably with a small measurement error, quickly and with extremely high sensitivity.
<13> The target detection substrate according to any one of <1> to <12>, wherein the interaction is at least one of physical adsorption and chemical adsorption.
<14> The target detection substrate according to any one of <2> to <13>, wherein the target interaction unit is a target capturing body capable of capturing a target.

  <15> Target capture body is enzyme, coenzyme, enzyme substrate, enzyme inhibitor, host compound, metal, antibody, antigen, microorganism, parasite, bacteria, virus, virus particle, cell, cell debris, metabolite, Nucleic acid, hormone, hormone receptor, lectin, sugar, bioactive substance, bioactive substance receptor, allergen, protein, blood protein, tissue protein, nuclear substance, neurotransmitter, hapten, drug, environmental substance, chemical species and these The target detection substrate according to <14>, which is at least one selected from derivatives. When the detection substrate according to <15> is an enzyme substrate or an enzyme inhibitor, and the target capturing body is the coenzyme, enzyme substrate or enzyme inhibitor, the target is, for example, the coenzyme. Is a coenzyme, an enzyme substrate or an enzyme inhibitor, and the target capturing body is the inclusion compound, the target is, for example, a guest compound of the inclusion compound, and the target capturing body is When it is a metal, the target is, for example, the inclusion compound, and when the target compound is the antibody, the target is, for example, the antigen, virus, cell debris, or metabolite of the antibody. When a protein is used as an antigen, the target is, for example, a nucleic acid complementary to the nucleic acid, and when the target capturing body is the hormone receptor, the target is, for example, a host that is received by the hormone receptor. When the target capturing body is the lectin, the target is, for example, a sugar that is received by the lectin, and when the target capturing body is the physiologically active substance receptor, the target Is, for example, a physiologically active substance that is received by the physiologically active substance receptor.

<16> The host compound is selected from a monomolecular host compound, a multimolecular host compound, a polymer host compound, and an inorganic host compound,
The monomolecular host compound is selected from cyclodextrin, crown compound, cyclophane, azacyclophane, calixarene, cyclotriperatrilene, spherand, cavitand, and cyclic oligopeptide;
The multimolecular host compound is selected from urea, thiourea, deoxycholic acid, perhydrotriphenylene, tri-o-thymotide,
The polymeric host compound is selected from cellulose, starch, chitin, chitosan and polyvinyl alcohol;
The substrate for target detection according to <15>, wherein the inorganic host compound is selected from an intercalation compound, zeolite, and Hofmann type complex.

<17> The target detection substrate according to any one of <14> to <16>, wherein the target is avidin, and at least one of the target receptor and the target capture body is biotin.
<18> The target detection substrate according to any one of <1> to <17>, wherein the liquid material contains a polar solvent. In the base material for target detection as described in <18>, the said liquid substance has an affinity with the said target interaction layer because it is the said polar solvent. Therefore, when the polar solvent and the target interaction layer come into contact with each other, the thickness of the target interaction layer itself is increased. As a result, it is suitable for various detections in which the target is detected easily, reliably, rapidly and with extremely high sensitivity with a small measurement error.
<19> The polar solvent is at least one selected from water, alcohol, ester group-containing solvent, amide group-containing solvent, carboxylic acid group-containing solvent, sulfonic acid group-containing solvent, amine-containing solvent, and nitrile group-containing solvent. It is the base material for target detection as described in said <18>.
<20> The target detection substrate according to any one of <4> to <19>, wherein the layer adjacent to the target interaction layer is a light interference layer.
<21> The target detection base material according to <20>, which includes a base material, a light interference layer, and a target interaction layer in this order.
<22> The optical interference layer according to any one of <20> to <21>, wherein the optical interference layer includes at least one different refractive index film having a different refractive index different from that of at least one of the base material and the target interaction layer. This is a target detection substrate. In the target detection substrate according to <22>, the optical interference layer includes a different refractive index film having a refractive index different from at least one of the refractive index of the substrate and the target interaction layer. Therefore, it is possible to radiate interference light with a sharp spectral curve, and to detect easily, reliably, quickly and with extremely high sensitivity even when there is only a slight wavelength change of the interference light. Can do.

<23> The base material for target detection according to <22>, which has two or more different refractive index films, and the refractive indexes of the two or more different refractive index films are different. The base material for target detection according to <23> has two or more different refractive index films, and the refractive indexes of the two or more different refractive index films are different from each other. Even when there is only a slight wavelength change (wavelength shift) of the interference light, it can be detected easily, reliably, quickly and with extremely high sensitivity.
<24> The target detecting substrate according to <22> to <23>, wherein the different refractive index film is a dielectric film.
<25> The target detection substrate according to any one of <22> to <24>, wherein the different refractive index film contains an oxygen compound.
<26> The target detection substrate according to <25>, wherein the oxygen compound is at least one selected from metal oxides and nonmetal oxides.
<27> The target detection base material according to <26>, wherein the metal oxide is at least one selected from Ta ₂ O ₅ , TiO _2, and SiO ₂ .
<28> The target detection substrate according to any one of <22> to <27>, wherein the thickness of the different refractive index film is 0.01 to 100 μm.
<29> The target detection substrate according to any one of <22> to <28>, wherein the density of the different refractive index film is 1.0 to 3.0 g / cm ³ .

<30> Any of the above <20> to <21>, wherein the optical interference layer has at least one layer of the same refractive index film having a refractive index substantially equal to the refractive index of at least one of the base material and the target interaction layer The substrate for target detection described in 1.
<31> The target detection substrate according to any one of <20> to <30>, wherein the optical interference layer is an interference filter. In the target detection substrate according to <31>, since the optical interference layer is an interference filter, interference light having a sharp spectrum curve can be emitted, and a slight wavelength change ( Even if there is only a wavelength shift), it can be detected easily and reliably, quickly and with extremely high sensitivity, and the thickness of the target interaction layer on the surface can be reduced, so that target detection It is easy to manufacture a base material for use.
<32> The base material for target detection according to any one of <21> to <31>, wherein the base material is formed of at least one selected from semiconductors, ceramics, metals, glass, quartz glass, and plastics. It is.

<33> Light irradiation means for irradiating light, light interference means capable of changing the wavelength of interference light of light irradiated by the light irradiation means by interacting with a target, and provided in the path of the interference light Wavelength change detecting means for detecting a wavelength change of the interference light emitted by the optical interference means, and the optical interference means is for detecting a target according to any one of <1> to <32>. a substrate, a target detection device, characterized in that target interaction layer in target detection substrate is capable of interacting with the target. In the target detection apparatus according to <33>, the light irradiation unit irradiates light. When the target interaction layer in the optical interference means comes into contact with the liquid material, the thickness of the target interaction layer is increased. When the target is present in the liquid, the target interacts with the target interaction layer in the optical interference means, thereby increasing the thickness of the target interaction layer, and Even after the liquid material is removed, the shrinkage of the film thickness is suppressed, and the wavelength of the interference light of the light irradiated by the light irradiation means changes (wavelength shift). The wavelength change detection means detects the interference light whose wavelength has changed (wavelength shift), so that the target can be detected simply, reliably, quickly and with extremely high sensitivity with a small measurement error.

<34> The target detection apparatus according to <33>, wherein the light irradiation unit is capable of irradiating a linear light flux. In the target detection apparatus according to <34>, it is easy to control an incident angle of the light irradiated by the light irradiation unit to the light interference unit, and the light irradiation unit in the light interference unit area of the light receiving surface of the light irradiated can be designed small by, also be designed to have a small area of the light-receiving surface in the wavelength change detecting means for detecting a wavelength change of the interference light by the optical interference unit Measurement noise can be suppressed, and measurement errors are small.

  <36> The target detection according to any one of <33> to <35>, wherein the wavelength change detection unit can transmit only light having a specific wavelength and can detect that light having the specific wavelength has been transmitted. Device. In the target detection apparatus according to <36>, since the wavelength change detection unit can transmit only light of a specific wavelength, the wavelength change detection unit can cause the target to interact with the optical interference unit. And before the thickness of the target interaction layer is increased, it is impossible to transmit the interference light in the previous period, and after the thickness of the target interaction layer is increased, the interference light can be transmitted, or Before the thickness of the target interaction layer is increased, the interference light can be transmitted, and after the thickness of the target interaction layer is increased, the interference light cannot be transmitted. Even if it is extremely difficult to detect a slight change by only measuring a spectrum curve, that is, even if there is only a very slight wavelength change (wavelength shift), it can be detected easily and reliably. When the wavelength change detecting means detects the transmission of the interference light, the wavelength change of the interference light is detected, the optical interference means interacts with the target, and the target is easily and reliably detected with a small measurement error. Moreover, it is detected quickly and with extremely high sensitivity.

  <37> The target detection device according to any one of <33> to <36>, wherein the wavelength change detection unit is a light detection sensor capable of detecting an interference filter and transmitted light transmitted through the interference filter. In the target detection apparatus according to <37>, the interference filter may be configured such that the interference light is not allowed to pass through before the target interacts with the optical interference means and the thickness of the target interaction layer is increased. And, after the thickness of the target interaction layer is increased, by allowing the interference light to be transmitted, or before the thickness of the target interaction layer is increased, the interference light can be transmitted, After the thickness of the target interaction layer increases, by making the interference light impermeable, it is extremely difficult to detect a slight change only by measuring a normal spectral curve, that is, very little. Even if there is only a simple wavelength change (wavelength shift), it can be detected easily and reliably. When the wavelength change detection means detects the interference light, the wavelength change of the interference light is detected, the optical interference means interacts with the target, the target can be easily and reliably measured with a small measurement error, Moreover, it is detected quickly and with extremely high sensitivity. As a result, even if a very slight wavelength change occurs, the light detection sensor can detect the change as the transmitted light of the interference light, and thus the sensitivity is extremely high. In addition, after the target interacts with the optical interference means, the interference filter can transmit the interference light of a specific wavelength whose wavelength has changed, or the target interacts with the optical interference means. If the interference light having a specific wavelength can be transmitted before the measurement, the light detection sensor can measure the amount of the interference light to be quantified.

  <38> Any one of <33> to <37>, wherein the wavelength change detection unit is capable of measuring the spectrum of the interference light before the wavelength change and the spectrum of the interference light after the wavelength change and measuring the difference spectrum. A target detection apparatus according to claim 1. In the target detection apparatus according to <38>, when it is extremely difficult to detect a slight change by only measuring a normal spectral curve, that is, when there is only a very small wavelength change (wavelength shift). Even so, it can be detected easily and reliably, and the wavelength change detection means measures the spectral difference before and after the wavelength change of the interference light, that is, the difference spectrum, so that each is not a broad spectrum curve, An extremely sharp spectral curve is obtained and can be amplified. Then, the wavelength change of the interference light can be converted into the spectral intensity, and can be arbitrarily amplified. As a result, even if there is a slight wavelength change, it can be amplified and detected as spectral intensity, so it is highly sensitive, and the target is also quantified by the intensity of the spectral intensity. Can do.

<39> The target detection device according to <38>, wherein the wavelength change detection unit is capable of converting the difference spectrum into a spectrum intensity and amplifying the spectrum intensity. In the target detection apparatus according to <39>, when it is extremely difficult to detect a slight change only by measuring a normal spectrum curve, that is, when there is only a very slight wavelength change (wavelength shift). However, it can be detected easily and reliably, and the wavelength change detecting means can obtain a very sharp spectrum curve instead of a broad spectrum curve, and further, the wavelength change of the interference light can be obtained. Can be converted to spectral intensities, which can optionally be amplified. As a result, even if there is a slight wavelength change, it can be amplified and detected as spectral intensity, so it is highly sensitive, and the target can be easily quantified by the intensity of the spectral intensity. It can be carried out.
<40> The target detection device according to any one of <38> to <39>, wherein the wavelength change detection unit is a spectrophotometer.

<41> the light is irradiated to the target detection substrate according to any one of <32> to <1>, the target interaction layer definitive in target detection substrate is contacted with a liquid product, the target And a light irradiation step for causing the target interaction layer to interact and emitting the irradiated light as interference light, and a wavelength change detection step for detecting a wavelength change of the interference light. It is a detection method. In the target detection method according to <41>, in the light irradiation step, when the target is not present, the thickness of the target interaction layer increased by contact with the liquid material is such that the liquid material It is shrunk by removing. When the target is present, the target and the target interaction layer interact to increase the thickness of the target interaction layer, and even if the liquid is removed, the shrinkage of the film thickness Is suppressed. As a result, when the target exists, as a result of the change in the film thickness, the interference light of the irradiated light undergoes a wavelength change (wavelength shift) before and after the change in the film thickness, and the wavelength change detection step The target is detected by detecting the interference light having the wavelength change (wavelength shift).

  According to the present invention, conventional problems can be solved, and the present invention can easily and quickly measure various targets such as pathogenic substances, biological substances, and toxic substances without using an expensive measuring device or the like. In addition, it is possible to provide a target detection apparatus and a target detection method that can be detected with extremely high sensitivity and efficiency, and that can be quantified, and a target detection substrate that can be suitably used for them.

(Target detection device)
The target detection apparatus of the present invention includes light irradiation means, light interference means, and wavelength change detection means, and further includes other means as necessary.

<Light irradiation means>
The light irradiation means is not particularly limited as long as it has a function of irradiating light, and can be appropriately selected from known ones according to the purpose. Examples thereof include a light source such as a halogen lamp (for example, a xenon lamp). It is done.
Among these, those capable of irradiating a linear light beam are preferable. In this case, it is easy to control the incident angle of the light irradiated from the light irradiation means to the light interference means, and the area of the light receiving surface in the light interference means of the light irradiated from the light irradiation means is reduced. It can be designed to be small, the area of the light receiving surface in the wavelength change detecting means for detecting the wavelength change of the interference light by the optical interference means can be designed to be small, measurement noise can be suppressed, measurement error This is advantageous in that it can be reduced.
In the present invention, when a spectrophotometer is used as the wavelength change detecting means, a light source built in the spectrophotometer can be used as the light irradiating means.

<Optical interference means>
The light interference unit is a unit that can change the wavelength of the interference light of the light irradiated by the light irradiation unit by interacting with the target. The optical interference means includes the target detection base material of the present invention, and further includes other members as necessary.

―Base material for target detection―
In the target detection substrate of the present invention, the interference of the irradiated light is emitted as interference light, and the wavelength of the interference light can be changed after interacting with the target. It has a working layer and other layers appropriately selected.

―Target interaction layer―
As the target interaction layer, the thickness of the target interaction layer after the target detection substrate (the target interaction layer) is in contact with the liquid material and interacts with the target is determined as follows. Greater than the thickness of the target interaction layer prior to contact.

  The target interaction layer is not particularly limited as long as the above conditions are satisfied. For example, the thickness of the target interaction layer after contact with the liquid and the interaction between the target and the target interaction layer ( X) and the thickness (Y) of the target interaction layer before coming into contact with the liquid material may be referred to as the following formula: [(XY) / Y] × 100 (hereinafter referred to as “increase rate”) )> 50% is preferably satisfied. If the increase rate is less than 50%, the detection sensitivity may be lowered, and the measurement error becomes large, which is not preferable.

  The target interaction layer preferably includes a filament having a plurality of target interaction portions capable of interacting with a target. The filamentous body stands up in contact with the liquid material, and in this state, the thickness for the interaction between the target interaction portion and the target in the filamentous material is increased. Thus, the target interaction part that is present inside the target interaction layer and does not appear on the surface before contacting with the liquid is also contacted with the target after contacting with the liquid. It becomes possible to interact. As a result, it is advantageous that the target can be detected easily and reliably with a small measurement error, and quickly and with extremely high sensitivity.

-Filament-
The filamentous body is not particularly limited as long as it has a filamentous shape, and can be appropriately selected according to the purpose. Examples thereof include filamentous organic molecules and filamentous inorganic molecules.
These may be used alone or in combination of two or more. Among these, the filamentous organic molecule is preferable from the viewpoint of easy interaction with the target and easy molecular processing.

There is no restriction | limiting in particular as said filamentous organic molecule, According to the objective, it can select suitably, For example, a linear polymer, a helical polymer, etc. are mentioned.
The filamentous organic molecule is not particularly limited and may be appropriately selected depending on the intended purpose. Examples thereof include imine group-containing compounds, amine group-containing compounds, hydroxyl group-containing compounds, amide group-containing compounds, and carboxyl group-containing compounds. What has at least 1 type selected as a monomer unit is preferable.
Examples of the imine group-containing compound include methylimine, ethyleneimine, mitrilimine, sulfilimine, and the like.
Examples of the amine group-containing compound include vinylamine, allylamine, triethylenediamine, hexamethylenediamine, and the like.
Examples of the hydroxyl group-containing compound include vinyl alcohol.
Examples of the amide group-containing compound include acrylamide and methacrylamide.
Examples of the carboxyl group-containing compound include acrylate and methacrylate.

There is no restriction | limiting in particular as a number average molecular weight (Mn) of the said filamentous organic molecule, Although it can select suitably according to the objective, For example, 1,000-1,000,000 are preferable, 5,000-500 1,000 is more preferable.
There is no restriction | limiting in particular as a polymerization degree of the said filamentous organic molecule, Although it can select suitably according to the objective, For example, 20-10,000 are preferable and 100-1,000 are more preferable.

  The method for forming the filament is not particularly limited and may be appropriately selected depending on the intended purpose. However, the filament may be formed by adhering one end of the filament on a layer adjacent to the target interaction layer. preferable. In this case, since one end of the filamentous body is bonded, before contacting with the liquid material, the filamentous body is laid down while supporting the one end of the filamentous body, and after contacting the liquid material, It is erected while supporting the one end of the filamentous body. As a result, the target interaction layer comes into contact with the liquid material, thereby increasing the thickness of the target interaction layer itself, and the target and the target interaction layer interact to remove the liquid material. Even after this, shrinkage of the thickness in the target interaction layer is suppressed. For this reason, it is suitable for various detections in which the target is detected easily and reliably with a small measurement error, and quickly and with extremely high sensitivity.

-Linear polymer-
There is no restriction | limiting in particular as said polymer | macromolecule, According to the objective, it can select suitably, For example, what is represented by following Structural formula (1) to (7) is mentioned suitably. In the following structural formulas (1) to (7), X represents a divalent linking group, R represents the target interaction moiety, and m, n, and l represent integers. Me represents a methyl group.

The number average molecular weight (Mn) of the linear organic molecule is not particularly limited and may be appropriately selected according to purpose. For example, 1,000 to 1,000,000 is preferable, and 5,000 ~ 500,000 is more preferred.
There is no restriction | limiting in particular as a polymerization degree of the said filamentous organic molecule, Although it can select suitably according to the objective, For example, 20-10,000 are preferable and 100-1,000 are more preferable.

  The wavelength of the interference light by the light interference means may or may not be in the visible light range, but in the former case, it is preferable in that the interference light can be detected visually, and the wavelength change More preferably, the wavelength of the interference light later is in the visible light range. In this case, the interference light can be visually recognized as an interference color. The principle of the interference light being visually recognized is based on a so-called structural color development.

By appropriately selecting the number average molecular weight (Mn) and the degree of polymerization in the linear polymer, the target interaction layer has a previous film thickness that can efficiently interact with the target. Even after the interaction, the sufficient film thickness can be obtained, and further, the interference color of the interference light can be made visible by the principle of structural color development.

-Spiral polymer-
There is no restriction | limiting in particular as said helical polymer, According to the objective, it can select suitably, For example, (alpha) -helix polypeptide, DNA, amylose etc. are mentioned suitably.

The α-helix polypeptide is one of the secondary structures of the polypeptide, and rotates once for every 3.6 amino acid residues (forms 1 helix formation), and the imide group (—NH—) of the fourth amino acid. A hydrogen bond substantially parallel to the helical axis is formed between the carbonyl group (—CO—) and a energetically stable structure by repeating 7 amino acids as one unit.
The helical direction of the α-helix polypeptide is not particularly limited, and may be right-handed or left-handed. Naturally, there is only a right hand from the viewpoint of stability.

  The amino acid forming the α-helix polypeptide is not particularly limited as long as it has an α-helix structure, and can be appropriately selected according to the purpose, but it is easy to form the α-helix structure. Examples of such amino acids include aspartic acid (Asp), glutamic acid (Glu), arginine (Arg), lysine (Lys), histidine (His), glutamine (Gln), serine (Ser), threonine ( Preferred examples include Thr), alanine (Ala), cysteine (Cys), methionine (Met), tyrosine (Tyr), phenylalanine (Phe), tryptophan (Trp), and the like. These may be used individually by 1 type and 2 or more types may be used together.

The α-helix polypeptide can be designed to be hydrophilic, hydrophobic, or amphiphilic by appropriately selecting the amino acid. Preferred examples include serine (Ser), threonine (Thr), aspartic acid (Asp), glutamic acid (Glu), arginine (Arg), lysine (Lys), asparagine (Asn), and glutamine (Gln). In this case, the amino acid includes phenylalanine (Phe), tryptophan (Trp), isoleucine (Ile), tyrosine (Tyr), methionine (Met), leucine (Leu), valine (Val) and the like.
The amino acid constituting the α-helix polypeptide is not particularly limited, and may be any of L-amino acids, D-amino acids, derivatives in which side chain portions thereof are modified, and the like.

  In the α-helix polypeptide, a carboxyl group that does not constitute a peptide bond in the amino acid forming the α-helix can be made hydrophobic by esterification, Hydrolysis can be achieved by hydrolyzing the esterified carboxyl group.

The number average molecular weight (Mn) of the α-helix polypeptide is not particularly limited and may be appropriately selected depending on the intended purpose. For example, it is preferably 1,000 to 1,000,000, 000 to 500,000 is more preferable.
The degree of polymerization of the α-helix polypeptide is not particularly limited and may be appropriately selected depending on the intended purpose. For example, it is preferably 20 to 10,000, and more preferably 20 to 1,000.

By appropriately selecting the number average molecular weight (Mn) and the degree of polymerization in the α-helix polypeptide, the target interaction layer allows the target interaction layer to efficiently interact with the target, Even after the interaction, the sufficient film thickness can be obtained, and further, the interference color of the interference light can be made visible light by the principle of structural color development. It is advantageous in that the detection can be confirmed with.

  Specific examples of the α-helix polypeptide include poly (γ-methyl-L-glutamate), poly (γ-ethyl-L-glutamate), poly (L-glutamic acid-γ-benzyl), poly (n- Polyglutamic acid derivatives such as hexyl-L-glutamate), polyaspartic acid derivatives such as poly (β-benzyl-L-aspartate), poly (L-leucine), poly (L-alanine), poly (L-methionine) , Poly (L-phenylalanine), poly (L-lysine), poly (γ-methyl-L-glutamate), and the like.

  As said alpha-helix polypeptide, what was synthesize | combined thru | or suitably according to the method as described in well-known literature etc. may be used, and a commercial item may be used.

As one synthesis example of the α-helix polypeptide, a synthesis example of a block copolypeptide [poly (L-lysine) ₂₅ -poly (γ-methyl-L-glutamate) ₆₀ ] PLLZ ₂₅ -PMLG ₆₀ is shown as follows. It is as follows. That is, the block copolypeptide [poly (L-lysine) ₂₅ -poly (γ-methyl-L-glutamate) ₆₀ ] PLZZ ₂₅ -PMLG ₆₀ uses n-hexylamine as an initiator, as shown by the following formula: Polymerization of N ^ε carbobenzosi-L-lysine-N ^α -carboxic anhydride (LLZ-NCA), followed by polymerization of γ-methyl-L-glutamate-N-carboxic anhydride (MLG-NCA) Can be synthesized.

The synthesis of the α-helix polypeptide is not limited to the above method, and a genetic engineering method can also be performed. For example, a host cell is transformed with an expression vector incorporating a DNA encoding the target polypeptide. The transformant can be cultured for example.
Examples of the expression vector include a plasmid vector, a phage vector, a plasmid-phage chimeric vector, and the like.
Examples of the host cell include prokaryotic microorganisms such as Escherichia coli and Bacillus subtilis, eukaryotic microorganisms such as yeast, and animal cells.

  The α-helix polypeptide may be prepared by cutting out the α-helix structure portion from a natural fibrous protein such as α-keratin, myosin, epidermin, fibrinogen, tropomycin, silk fibroin and the like.

-DNA-
The DNA may be single-stranded DNA, but is preferably double-stranded DNA in that it can maintain a rod shape stably.
The double-stranded DNA has a double helical structure formed by positioning two right-handed helical strands extending in opposite directions around one central axis.
The polynucleotide chain is composed of four types of nucleobases: adenine (A), thymine (T), guanine (G), and cytosine (C). In the polynucleotide chain, the nucleobase is centered on the central axis. They exist in a form that protrudes inward in a plane perpendicular to each other, forming so-called Watson-Crick base pairs, specifically thymine for adenine and cytosine for guanine. Are connected. As a result, in the double-stranded DNA, the two polypeptide chains are complementarily bound to each other.

  The DNA can be controlled by a known PCR (Polymerase Chain Reaction) method, LCR (Ligase chain Reaction) method, 3SR (Self Sustained Sequence Replication) method, SDA (Strand Displacement Amplification) method, etc. Of these, the PCR method is preferred.

The DNA may be prepared by directly excising enzymatically from a natural gene with a control enzyme, may be prepared by a gene cloning method, or may be prepared by a chemical synthesis method.

In the case of the gene cloning method, for example, a normal nucleic acid amplified product is incorporated into a vector selected from a plasmid vector, a phage vector, a plasmid-phage chimeric vector, etc., and prokaryotic microorganisms such as Escherichia coli and Bacillus subtilis, yeast and the like Large quantities can be prepared by introducing any amplifiable host selected from eukaryotic microorganisms, animal cells and the like.
Examples of the chemical synthesis method include a liquid phase method such as triester method and phosphorous acid method, or a solid phase synthesis method using an insoluble carrier. In the case of the chemical synthesis method, a double-stranded DNA can be prepared by preparing a large amount of single-stranded DNA using a known automatic synthesizer and then performing annealing.

The amylose is a polysaccharide having a helical structure in which D-glucose constituting starch, which is a homopolysaccharide for storage of higher plants, is connected in a straight chain with α-1,4 bonds.
The number average molecular weight (Mn) of the amylose is not particularly limited and may be appropriately selected depending on the intended purpose. For example, 1,000 to 1,000,000 is preferable, and 1,000 to 500,000. Is more preferable.
There is no restriction | limiting in particular as a polymerization degree of the said amylose, Although it can select suitably according to the objective, For example, 10-5,000 are preferable and 10-1,000 are more preferable.

  By appropriately selecting the number average molecular weight (Mn) and the degree of polymerization in the amylose, the target interaction layer can have a previous film thickness that can efficiently interact with the target, Even after the interaction, the sufficient film thickness can be obtained, and furthermore, the interference color of the interference light can be made visible by the principle of structural color development, and the detection can be confirmed visually. This is advantageous.

The amylose may be commercially available or appropriately prepared according to a known method.
The amylose may contain amylopectin in a part thereof.

  The diameter of the rod-like organic molecule is not particularly limited, but is about 0.8 to 2.0 nm in the case of the α-helix polypeptide.

  The rod-like organic molecule may be entirely lipophilic (hydrophobic) or hydrophilic, part of which is lipophilic or hydrophilic, and the other part has an affinity opposite to that of the part. Although it may be amphiphilic, it is preferably hydrophilic.

  The target interaction unit is not particularly limited as long as it can interact with the target, but is preferably a target capturing body capable of capturing the target in the first stage.

  The target capturing body is not particularly limited as long as the target can be captured, and can be appropriately selected according to the purpose. For example, an enzyme, a coenzyme, an enzyme substrate, an enzyme inhibitor, a host compound, Metal, antibody, antigen, microorganism, parasite, bacteria, virus, virus particle, cell, cell debris, metabolite, nucleic acid, hormone, hormone receptor, lectin, sugar, bioactive substance, bioactive substance receptor, avidin, Examples include biotin, allergen, protein, blood protein, tissue protein, nuclear substance, neurotransmitter, hapten, drug, environmental substance, chemical species, and derivatives thereof. The target capturing bodies may be the same as or different from each other.

    There is no restriction | limiting in particular as an aspect of the said capture | acquisition, According to the objective, it can select suitably, For example, physical adsorption, chemical adsorption, etc. are mentioned. These can be formed by, for example, hydrogen bonds, intermolecular forces (van der Waals forces), coordination bonds, ionic bonds, covalent bonds, and the like.

  As the target, when the target capturing body is the enzyme, for example, it is a coenzyme of the enzyme, and when it is the coenzyme, for example, an enzyme having the coenzyme as a coenzyme. In the case of the host compound, for example, it is a guest compound (component to be included) of the host compound, and in the case of the antibody, for example, it is a protein as an antigen of the antibody, In the case of a protein, for example, an antibody having the protein as an antigen, and in the case of the nucleic acid, for example, a nucleic acid complementary to the nucleic acid, tubulin, chitin, etc. In some cases, for example, a hormone that is received by the hormone receptor, and in the case of the lectin, for example, a sugar that is received by the lectin, and the physiologically active substance When a receptor is, for example, a physiologically active substance that is received in the physiologically active substance receptors.

  The sample containing the target is not particularly limited and can be appropriately selected according to the purpose. Examples thereof include pathogens such as bacteria and viruses, blood isolated from living organisms, saliva, tissue lesions, etc. And excreta. Furthermore, when performing a prenatal diagnosis, fetal cells existing in amniotic fluid or a part of dividing egg cells in a test tube can be used as a sample. In addition, these samples are concentrated as a sediment directly or if necessary by centrifugation or the like, and then subjected to cell destruction treatment by, for example, enzyme treatment, heat treatment, surfactant treatment, ultrasonic treatment, a combination thereof or the like. You may use what gave beforehand.

  The host compound is not particularly limited as long as it has molecular recognition ability (host-guest binding ability), and can be appropriately selected according to the purpose. For example, a compound having a cylindrical (one-dimensional) cavity, Preferred examples include those having a layered (two-dimensional) cavity and those having a cage (three-dimensional) cavity.

  Examples of the host compound having a cylindrical (one-dimensional) cavity include urea, thiourea, deoxycholic acid, dinitrodiphenyl, dioxytriphenylmethane, triphenylmethane, methylnaphthalene, spirochroman, PHTP (perhydrotriphenylene). ), Cellulose, amylose, cyclodextrin (however, in the solution, the cavity is cage-shaped).

Examples of the target that can be captured by urea include n-paraffin derivatives.
Examples of the target that can be captured by thiourea include branched or cyclic hydrocarbons.
Examples of the target that can be captured by deoxycholic acid include paraffins, fatty acids, aromatic compounds, and the like.
Examples of the target that can be captured by dinitrodiphenyl include diphenyl derivatives.

Examples of the target that can be captured by dioxytriphenylmethane include paraffins, n-alkenes, squalene, and the like.
Examples of targets that can be captured by triphenylmethane include paraffins.
The methylnaphthalene is as the capturable targets, for example, n- paraffins to C _16, branched paraffins, and the like.
Examples of the target that can be captured by the spirochroman include paraffins.
Examples of targets that can be captured by PHTP (perhydrotriphenylene) include chloroform, benzene, various polymer substances, and the like.
Examples of targets that can be captured by cellulose include H ₂ O, paraffins, CCl ₄ , dyes, iodine, and the like.
Examples of targets that can be captured by amylose include fatty acids and iodine.

The cyclodextrin is a cyclic dextrin produced by the degradation of starch with amylase, and three types of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin are known. In the present invention, the cyclodextrin includes cyclodextrin derivatives in which a part of these hydroxyl groups are changed to other functional groups such as alkyl groups, allyl groups, alkoxy groups, amide groups, sulfonic acid groups, and the like. .

  Examples of targets that can be captured by the cyclodextrin include thymol, eugenol, resorcin, ethylene glycol monophenyl ether, phenol derivatives such as 2-hydroxy-4-methoxy-benzophenone, salicylic acid, methyl paraoxybenzoate, ethyl paraoxybenzoate, and the like. Benzoic acid derivatives and esters thereof, steroids such as cholesterol, vitamins such as ascorbic acid, retinol and tocopherol, hydrocarbons such as limonene, allyl isothiocyanate, sorbic acid, iodine molecule, methyl orange, Congo red, 2-p -Toluidinylnaphthalene-6-sulfonic acid potassium salt (TNS), and the like.

  Examples of the layered (two-dimensional) host compound include clay mineral, graphite, smectite, montmorillonite, zeolite, and the like.

Examples of targets that can be captured by the clay mineral include hydrophilic substances and polar compounds.
Examples of the target that can be captured by graphite include O, HSO ₄ ⁻ , halogen, halide, and alkali metal.
Examples of targets that can be captured by montmorillonite include brucine, codeine, o-phenylenediamine, benzidine, piperidine, adenine, guanine, and their liposides.
Examples of targets that can be captured by the zeolite include H ₂ O.

  Examples of the cage (three-dimensional) host compound include hydroquinone, gaseous hydrate, tri-o-thymotide, oxyflavan, dicyanoammine nickel, cryptand, calixarene, and crown compound.

Examples of targets that can be captured by hydroquinone include HCl, SO ₂ , acetylene, and rare gas elements.
Examples of targets that can be captured by the gaseous hydrate include halogens, rare gas elements, and lower hydrocarbons.
Examples of targets that can be captured by tri-o-thymotide include cyclohexane, benzene, chloroform, and the like.
Examples of the target that can be captured by the oxyflavan include an organic base.
Examples of targets that can be captured by the dicyanoammine nickel include benzene and phenol.
Examples of targets that can be captured by the cryptand include NH ⁴⁺ and various metal ions.

  The calixarene is a cyclic oligomer in which a phenol unit synthesized from phenol and formaldehyde under appropriate conditions is bonded with a methylene group, and 4 to 8 nuclei are known. Among these, examples of targets that can be captured by pt-butylcalixarene (n = 4) include chloroform, benzene, and toluene. Examples of targets that can be captured by pt-butylcalixarene (n = 5) include isopropyl alcohol and acetone. Examples of targets that can be captured by pt-butylcalixarene (n = 6) include chloroform, methanol, and the like. Examples of the target that can be captured by pt-butylcalixarene (n = 7) include chloroform.

  The crown compound includes not only a crown ether having oxygen as an electron-donating donor atom, but also a macrocycle compound having a donor atom such as nitrogen or sulfur as a ring structure constituent atom as an analog thereof, and also represents a cryptand. Are also included, for example, cyclohexyl-12-crown-4, dibenzo-14-crown-4, t-butylbenzo-15-crown-5, dibenzo-18-crown. -6, dicyclohexyl-18-crown-6, 18-crown-6, tribenzo-18-crown-6, tetrabenzo-24-crown-8, dibenzo-24-crown-6, and the like.

Examples of targets that can be captured by the crown compound include various metal ions such as alkali metals such as Li, Na and K, alkaline earth metals such as Mg and Ca, NH ⁴⁺ , alkylammonium ions, guanidinium ions, Aromatic diazonium ions and the like, and the crown compound forms a complex with them. In addition to these, targets that can be captured by the crown compound include C—H (acetonitrile, malonnitrile, adiponitrile, etc.), NH (aniline, aminobenzoic acid, amide, sulfamide) having relatively high acidity. Derivatives), polar organic compounds having O—H (phenol, acetic acid derivatives, etc.) units, etc., and the crown compound forms a complex with these.

  The size (diameter) of the cavity of the host compound is not particularly limited and can be appropriately selected according to the purpose. From the viewpoint of exhibiting stable molecular recognition ability (host-guest binding ability), The thickness is preferably 0.1 nm to 2.0 nm.

The host compound can also be classified into, for example, a monomolecular host compound, a multimolecular host compound, a polymer host compound, an inorganic host compound, and the like.
Examples of the monomolecular host compound include cyclodextrin, crown compound, cyclophane, azacyclophane, calixarene, cyclotriperatrilene, spherand, cavitand, cyclic oligopeptide, and the like.
Examples of the multimolecular host compound include urea, thiourea, deoxycholic acid, perhydrotriphenylene, tri-o-thymotide, and the like.
Examples of the polymer host compound include cellulose, starch, chitin, chitosan, polyvinyl alcohol, and the like.
Examples of the inorganic host compound include intercalation compounds, zeolites, Hofmann type complexes, and the like.

The antibody is not particularly limited as long as it causes an antigen-antibody reaction specifically with the antigen, and may be, for example, a polyclonal antibody or a monoclonal antibody, and further, IgG, IgM, IgE, IgG Fab ′, Fab, F (ab ′) _{2 and the} like are also included.

  The antigen is not particularly limited and may be appropriately selected depending on the type of the antibody. For example, plasma protein, tumor marker, apoprotein, viral antigen, autoantibody, coagulation / fibrinolytic factor, hormone, blood Middle drugs, HLA antigens, and the like.

Examples of the plasma protein include immunoglobulins (IgG, IgA, IgM, IgD, IgE), complement components (C3, C4, C5, C1q), CRP, α ₁ -antitrypsin, α ₁ -microglobulin, β _2- microglobulin, haptoglobin, transferrin, ceruloplasmin, ferritin and the like.

  Examples of the tumor marker include α-fetoprotein (AFP), carcinoembryonic antigen (CEA), CA19-9, CA125, CA15-3, SCC antigen, prostatic acid phosphatase (PAP), PIVKA-II, γ-semi Noprotein, TPA, elastase I, nerve specific enolase (NSE), immunosuppressive acidic protein (IAP), and the like.

  Examples of the apoprotein include apo AI, apo A-II, apo B, apo C-II, apo C-III, and apo E.

Examples of the viral antigen include hepatitis B virus (HBV) -related antigen, hepatitis C virus (HVC) -related antigen, HTLV-I, HIV, rabies virus, influenza virus, rubella virus, and the like.
Examples of the HCV-related antigen include HCVc100-3 recombinant antigen, pHCV-31 recombinant antigen, pHCV-34 recombinant antigen, and a mixture thereof can be preferably used. Examples of the HIV-related antigen include virus surface antigens, and the like, for example, HIV-I env. gp41 recombinant antigen, HIV-I env. gp120 recombinant antigen, HIV-I gag. p24 recombinant antigen, HIV-II env. p36 recombinant antigen, and the like.
Examples of infectious diseases other than viruses include MRSA, ASO, toxoplasma, mycoplasma, STD, and the like.

  Examples of the autoantibodies include anti-microsomal antibodies, anti-thyroglobulin antibodies, antinuclear antibodies, rheumatoid factors, anti-mitochondrial antibodies, and myelin antibodies.

Examples of the coagulation / fibrinolytic factor include fibrinogen, fibrin degradation product (FDP), plasminogen, α ₂ -plasmin inhibitor, antithrombin III, β-thromboglobulin, factor VIII, protein C, protein S, and the like. It is done.

Examples of the hormone include pituitary hormone (LH, FSH, GH, ACTH, TSH, prolactin), thyroid hormone (T ₃ , T ₄ , thyroglobulin), calcitonin, parathyroid hormone (PTH), adrenal cortex hormone (aldosterone). , Cortisol), gonadal hormones (hCG, estrogen, testosterone, hPL), pancreatic / gastrointestinal hormones (insulin, C-peptide, glucagon, gastrin), others (renin, angiotensin I, II, enkephalin, erythropoietin), etc. It is done.

  Examples of the blood drug include antiepileptic drugs such as carbamazepine, primidone and valproic acid, cardiovascular disease drugs such as digoxin, quinidine, digitoxin and theophylline, and antibiotics such as gentamicin, kanamycin and streptomycin.

  Preferred examples of the protein include low molecular weight (about 6000 to 13000) having a high affinity for many heavy metals, particularly zinc, cadmium, copper, mercury and the like. These proteins are present in animal liver, kidney, and other tissues, and have recently been found to be present in microorganisms. In addition, these proteins have a high cysteine content and an amino acid distribution that contains almost no aromatic residues, and are substances having a detoxifying function such as cadmium and mercury in vivo, as well as zinc and copper. It is an important substance that is also involved in the storage of trace metals essential for living bodies and the distribution in living bodies.

Examples of the heavy metal include alkylmercury compound (R-Hg), mercury or its compound (Hg), cadmium or its compound (Cd), lead or its compound (Pb), hexavalent chromium (Cr ⁶⁺ ), copper or The compound (Cu), zinc or the compound (Zn), cyan, hexavalent chromium, arsenic, selenium, manganese, nickel, iron, zinc, selenium, tin, and the like can be given.

There is no restriction | limiting in particular as said adhesion | attachment, Although it can select suitably according to the objective, For example, a chemical bond etc. are mentioned suitably.
There is no restriction | limiting in particular as said chemical bond, It can select suitably from well-known chemical bonds, For example, a covalent bond, an ionic bond, a coordination bond, a hydrogen bond, etc. are mentioned.

The method for adhering the layer adjacent to the target interaction layer and the filamentous material is not particularly limited and may be appropriately selected depending on the intended purpose. For example, it can be adhered by the following method. .
That is, when the linear organic molecule represented by the structural formula (1) is used as the filamentous organic molecule, carboxylic acid amidation, sulfonic acid amidation with an acidic residue, or ring-opening polymerization of aziridine It can be adhered by use as a starting terminal at times, covalent bonds such as alkylation with halides, arylation, and ionic bonds with compounds having acidic functional groups.

There is no restriction | limiting in particular as said liquid substance, Although it can select suitably according to the objective, For example, a polar solvent, a nonpolar solvent, etc. are mentioned.
The polar solvent is not particularly limited as long as it is polar, and can be appropriately selected according to the purpose. Examples thereof include water, alcohol, ester group-containing solvent, amide group-containing solvent, carboxylic acid group-containing solvent, sulfone. Examples include acid group-containing solvents, amine group-containing solvents, and nitrile group-containing solvents.
The nonpolar solvent is not particularly limited as long as it is nonpolar, and can be appropriately selected according to the purpose. Examples thereof include benzene and carbon tetrachloride.

  From the viewpoint of affinity with the target interaction layer, when the filament has a hydrophilic group, the liquid material is preferably the polar solvent, and when the filament has a hydrophobic group. The liquid material is preferably a nonpolar solvent. In the case where the target interaction layer has a hydrophilic group and the liquid is a polar solvent, the target interaction layer comes into contact with the polar solvent, so that the target interaction layer is derived from their affinity. When the target interaction layer has a hydrophobic group and the liquid is a nonpolar solvent, the target interaction layer is in contact with the nonpolar solvent, From these affinities, the film thickness of the target interaction layer is increased.

-Other layers-
There is no restriction | limiting in particular as said other layer, According to the objective, it can select suitably, For example, a light interference layer, a base material, etc. are mention | raise | lifted.

  The optical interference layer is not particularly limited as long as, for example, the light irradiated by the light irradiation means in the previous period can be emitted as interference light. For example, the optical interference layer has at least one different refractive index film or copper refractive index film. Etc. are preferable.

  There is no restriction | limiting in particular as said base material, According to the objective, it can select suitably, For example, what was formed with a semiconductor, ceramics, a metal, glass, quartz glass, plastics etc. is mentioned suitably.

  There is no restriction | limiting in particular as a shape of the said base material, Although it can select suitably according to the objective, For example, it is preferable that it is plate shape.

  The position of the base material and the light interference layer is not particularly limited and can be appropriately selected depending on the purpose. For example, the base material (lowermost layer), the interference layer (intermediate layer), and the target interaction Examples include a layer (uppermost layer).

The different refractive index film is not particularly limited as long as it has at least any refractive index of the base material and the target interaction layer, and can be appropriately selected according to the purpose. The number may be two or more, and in the case of two or more, the refractive indexes of the different refractive index films may be different from each other. Specific examples of the different refractive index film include a dielectric film and a film containing an oxygen compound.
There is no restriction | limiting in particular as said oxygen compound, According to the objective, it can select suitably, For example, a metal oxide, a nonmetal oxide, etc. are mentioned.
The metal oxide is not particularly limited and may be appropriately selected depending on the intended purpose, _{e.g., Ta 2 O 5, TiO 2} , SiO 2, etc. are preferably exemplified. These may be used alone or in combination of two or more.
There is no restriction | limiting in particular as said nonmetallic oxide, According to the objective, it can select suitably.

  There is no restriction | limiting in particular as thickness (physical film thickness) of the said different refractive index film | membrane, Although it can select suitably according to the objective, For example, 0.01-100 micrometers is preferable and 0.01-10 micrometers is more preferable. 0.01 to 5.0 μm is particularly preferable. When the thickness is less than 0.01 μm, when measuring the interference light of the target detection substrate, ripples may not be generated in the graph of transmittance against wavelength change of the interference light, and exceeds 100 μm. In some cases, too much ripple is generated.

There is no restriction | limiting in particular as a density of the said different refractive index film | membrane, Although it can select suitably according to the objective, For example, 1.0-3.0 g / cm < ³ > is preferable, 1.2-2.6 g / cm ³ is more preferable, and 1.4 to 2.6 g / cm ³ is particularly preferable. When the density is less than 1.0 g / cm ³ , as a result of the birefringence film becoming brittle, durability of the optical interference base material itself may be inferior, and when exceeding 3.0 g / cm ³ , The number of ripples may be inappropriate and is not preferable.

  The method for forming the different refractive index film (the optical interference layer) is not particularly limited and may be appropriately selected depending on the purpose. For example, an EB (electron beam) vapor deposition method, an ion is applied to the surface of the substrate. Examples thereof include a film forming method by an assist method, an ion plating method, and the like.

  There is no restriction | limiting in particular as the number of layers of the said different refractive index film | membrane in the said optical interference layer, Although it can select suitably according to the objective, For example, the case where this different refractive index film | membrane is 1 layer is preferable. Even if the different refractive index film is a single layer, it interferes with the irradiated light and emits it as interference light. In the graph of transmittance against wavelength change of the interference light, a ripple is generated and the ripple is used. The detection based on the wavelength change can be detected easily and quickly with a small measurement error, without using an expensive measuring device, etc., and with extremely high sensitivity and efficiency. This is advantageous in that it can be mass-produced. Further, the wavelength of the interference light can be easily adjusted in the visible light range, and depending on the thickness and material of the oxide film, the interference light by the light interference layer may show an interference color. It can also be used as a “precolored substrate”.

  The same refractive index film is not particularly limited as long as it has a refractive index substantially equal to at least one of the refractive index of the base material and the target interaction layer, and is appropriately selected according to the purpose. Can do.

  The optical interference layer is preferably a layer formed on the substrate and adjacent to the target interaction layer.

―Structural coloring―
The structural color development is based on the multilayer thin film interference theory, which is the color development principle of morpho butterfly scales, and the structural color body (film, layer) has an electric field, magnetic field, temperature, light (eg natural light, infrared light, ultraviolet light), etc. When the external stimulus is applied, the color of light generated on the surface of the structural color body is the result of reflection of light of a specific wavelength according to the thickness of the structural color body (film, layer) and its refractive index. In the structural color development, no dye or pigment is required.

Here, the principle of the structural color development will be described below.
As shown in FIGS. 1 and 2, when the target interaction layer (or the refractive index film) is irradiated with light by the light irradiation means, the target interaction layer (or the different refractive index film) is used. The wavelength (λ) of the interference light is strengthened by the condition shown by the following formula (1) and is weakened by the condition shown by the following formula (2).

  In the formula (1) and the formula (2), λ means the wavelength (nm) of interference light, and α is the incident angle of light to the target interaction layer (or the different refractive index film). (Degree), t means the target interaction layer (or the thickness (nm) of the different refractive index film), and l denotes the number of the target interaction layer (or the different refractive index film). N means the refractive index of the target interaction layer (or the different refractive index film), and m means an integer of 1 or more.

  There is no restriction | limiting in particular as thickness of the said target interaction layer (or the said different refractive index film | membrane), Although it can select suitably according to the objective, 810 nm or less is preferable and 10-810 nm is more preferable. By appropriately changing the thickness, the color (wavelength) of the structural color can be changed.

  In the target detection substrate of the present invention, the film thickness of the target interaction layer is increased by contact with the liquid material, and the target interaction with the target interacts with the target interaction layer. The film thickness of the working layer increases, and even after the liquid material is removed, shrinkage of the film thickness is suppressed. For this reason, it can be used in various fields, and can detect various targets such as pathogenic substances, ecological substances, toxic substances, etc. easily and quickly with a small measurement error, and with extremely high sensitivity. In particular, it can be suitably used for the target detection apparatus of the present invention.

<Wavelength change detection means>
The wavelength change detection means is provided in a path of the interference light and has a function of detecting a wavelength change of the interference light.
The wavelength change detection means is not particularly limited as long as it has the above function, and can be appropriately selected according to the purpose. For example, (1) it can transmit only light of a specific wavelength, (2) the spectrum of the interference light before the wavelength change and the spectrum of the interference light after the wavelength change can be measured, and the difference spectrum can be measured; 3) In the graph of the transmittance with respect to the wavelength change of the interference light, the one measuring the wavelength change by using the ripple or the dominant wavelength in the graph, (4) the one measuring the change in the interference color visually. Preferably mentioned.

Among these, in the case of the above (1), the wavelength change detecting means can transmit only light of a specific wavelength, so that the wavelength change detecting means can cause the target to interact with the optical interference means. Before the thickness of the target interaction layer is increased by acting to make the interference light impermeable, and after the thickness of the target interaction layer is increased, the interference light can be transmitted, or The interference spectrum can be transmitted before the thickness of the target interaction layer is increased, and the interference spectrum cannot be transmitted after the thickness of the target interaction layer is increased. Even if it is extremely difficult to detect a slight change by measuring a curve, that is, even if there is only a very slight wavelength change (wavelength shift), it can be detected easily and reliably. When the wavelength change detecting means detects the transmission of the interference light, the wavelength change of the interference light is detected, and the optical interference means interacts with the target, that is, the presence of the target in a sample or the like. It is easily and conveniently detected with extremely high sensitivity.
Furthermore, the target can be quantified by the amount of transmitted light (intensity of transmitted light). That is, a calibration curve indicating the relationship between the amount of transmitted light of the interference light (transmitted light intensity) and the amount of capture of the target by the light interference means is prepared in advance, and the target containing the target is sampled. If the amount of transmitted interference light (intensity of transmitted light) is measured when the content is measured, the amount of the target captured by the optical interference means can be quantified from the calibration curve.

In the case of (2), the wavelength change detecting means measures the spectral difference before and after the change of the wavelength of the interference light, that is, the difference spectrum. Even when it is extremely difficult to detect the change, that is, when there is only a slight wavelength change (wavelength shift) and when the peak as the main wavelength is not formed in the transmittance graph of the interference light It can be detected easily and reliably, the wavelength change can be converted into spectral intensity, and the amplification can be arbitrarily performed. As a result, it is a very slight change in wavelength, and even when the peak as the main wavelength in the graph is not formed, it can be detected as an amplified spectral intensity, is highly sensitive, simple and Rapid and highly sensitive detection can be performed.
Furthermore, the target can be quantified by measuring the spectral intensity. That is, a calibration curve indicating the relationship between the transmitted light amount of the interference light (transmitted light intensity) and the wavelength intensity in the difference spectrum of the wavelength change (peak shift) of the interference light by the optical interference unit is prepared in advance. In addition, a calibration curve showing the relationship between the wavelength intensity of the difference spectrum and the amount of the target captured by the optical interference means (the target detection substrate) is prepared in advance, and contains the target. The amount of the target captured by the optical interference means from the calibration curve can be determined by measuring the wavelength intensity of the difference spectrum of the wavelength change (peak shift) of the interference light when measuring the target content of the sample to be measured. Can be quantified.

  As a specific example of the above (1), a combination of an interference filter and a light detection sensor capable of detecting transmitted light that has passed through the interference filter is preferably used. In this case, the interference filter does not transmit the interference light before the thickness of the target interaction layer is increased by interacting with the optical interference means, and the thickness of the target interaction layer is increased. Later, by allowing the interference light to pass through, or after allowing the interference light to pass before the thickness of the target interaction layer is increased, and after increasing the thickness of the target interaction layer By making the interference light incapable of transmitting, it is extremely difficult to detect a slight change only by measuring a normal spectrum curve, that is, when there is only a very slight wavelength change (wavelength shift). However, it can be detected easily and reliably, and when the wavelength change detecting means detects the transmission of the interference light, the wavelength change of the interference light is detected, and the optical interference means detects the target. Having interacted, that is, the presence of the target in a sample or the like can be easily and easily detected with extremely high sensitivity, and the light detection sensor detects the interference light transmitted through the interference filter, The change in the wavelength of the interference light is detected, and the fact that the optical interference means interacts with the target, that is, the presence of the target in a sample or the like is easily and easily detected with extremely high sensitivity. As a result, the light detection sensor detects the transmitted light of the interference light even when a very slight wavelength change occurs and the peak as the main wavelength is not formed in the graph. Can be very sensitive. In addition, the interference filter may not transmit the interference light before the thickness of the target interaction layer in the optical interference means is increased, or the interference filter after the thickness of the target interaction layer is increased. When light can be transmitted, the light detection sensor measures the amount of transmitted interference light, whereby the target can be quantified.

There is no restriction | limiting in particular as said interference filter, According to the objective, it can select suitably, You may use a commercial item.
The interference filter interferes only with incident light having a specific wavelength. Incident light having a wavelength other than the specific wavelength can be transmitted.
There is no restriction | limiting in particular as said light detection sensor, According to the objective, it can select suitably, For example, a CdS cell, a photodiode, a photoelectric tube, a pyroelectric sensor, a CCD sensor, a PSD sensor etc. are mentioned.
As a specific example of the above (2), a known spectrophotometer or the like can be preferably cited.

  In the target detection apparatus of the present invention, the light irradiation means irradiates light. The target interaction layer in the light interference means is brought into contact with the liquid material, thereby increasing the thickness of the target interaction layer. When the target is present in the liquid, the thickness of the target interaction layer is increased by interacting with the target interaction layer and the target in the optical interference means, and the liquid Even after the film is removed, the shrinkage of the film thickness is suppressed, and the wavelength of the interference light of the light irradiated by the previous light irradiation means changes (wavelength shift). When the wavelength changing means detects the interference light having the wavelength changed (wavelength shifted), the thickness of the target interaction layer itself increases, so that it can be used in various fields without using an expensive measuring instrument. It is possible to detect various targets such as pathogenic substances, biological substances, toxic substances, etc. efficiently and reliably and simply, and also can perform quantification of these, diagnosis apparatus, analysis apparatus, quantification apparatus Etc. can be suitably used.

<How to use the target detection device>
Below, the usage method of the target detection apparatus of this invention is demonstrated. In addition, since the target detection method of this invention can be performed suitably using this target detection apparatus, the target detection method of this invention is demonstrated through the usage method of this target detection apparatus.
The target detection method of the present invention includes a light irradiation step and a wavelength change detection step.

Hereinafter, an example of a method of using the target detection apparatus of the present invention will be described with reference to FIG. When the light interference means (the target detection substrate) is stored (dried), one end of the filamentous organic molecule 10 is adhered on the surface of the light interference layer 40, and the filamentous organic molecule 10 is laid sideways. Being done. In this state, a part of the target interaction part 10a provided in the side chain of the filamentous organic molecule 10 appears on the surface, and the other part exists inside. Next, when the optical interference means (the target detection substrate) is brought into contact with water as the liquid material, one end of the filamentous organic molecule 10 is attached to the optical interference layer 40 due to the affinity between the filamentous organic molecule 10 and water. As the thickness of the target interaction layer increases, the target interaction portion 10 a existing inside can also interact with the target 20. Furthermore, when the target 20 exists in the water, the target 20 and the target interaction unit 10a interact with each other, and the filamentous organic molecule 10 remains substantially upright even after the water is dried. The state is maintained, and as a result, the increased film thickness shrinkage of the target interaction layer is suppressed.
Therefore, when the target 20 is not present, the increased film thickness of the target interaction layer is shrunk with the evaporation of the water and returns to the original state. The increased film thickness of the working layer is suppressed from shrinkage. As a result, the wavelength of the interference light of the light irradiated from the light irradiation means is changed (wavelength shift) as the film thickness increases.
The interference light is emitted by irradiating the light interference means (the target detection substrate) with light so that the light interference means reflects or transmits the light.
The above is the light irradiation step in the target detection method of the present invention. In the light irradiation step, the target detection base material is irradiated with light, the target interaction layer (target interaction part) in the target detection base material is allowed to interact with the target, and the irradiated light Is emitted as interference light.

The detection of the wavelength change of the interference light is as described above with respect to the wavelength change detection means in the target detection device, and the wavelength change detection means can transmit only light of a specific wavelength. The wavelength change detecting means prevents the interference light from being transmitted before the target interacts with the light interference means and the thickness of the target interaction layer is increased, and the thickness of the target interaction layer increases. After the interference light can be transmitted, or before the thickness of the target interaction layer is increased, the interference light can be transmitted and the thickness of the target interaction layer is increased. In the case where the interference light cannot be transmitted, it is extremely difficult to detect a slight change by only measuring a normal spectrum curve, that is, a very slight wavelength change (wave Even if there is only a shift), it can be detected easily and reliably, and when the wavelength change detecting means detects the transmission of the interference light, the wavelength change of the interference light is detected, and the light That the interference means interacts with the target, that is, the presence of the target in a sample or the like is easily and easily detected with extremely high sensitivity.
Furthermore, the target can be quantified by the amount of transmitted light (intensity of transmitted light). That is, a calibration curve indicating the relationship between the amount of transmitted light (the intensity of transmitted light) of the interference light and the amount of the target captured by the light interference means (the target detection substrate) is prepared in advance, and the target When the content of the target is measured for a sample containing, the amount of transmitted interference light (intensity of transmitted light) is measured and captured by the optical interference means (the target detection substrate) from the calibration curve The amount of the target made can be quantified.

Further, since the wavelength change detecting means measures the spectral difference before and after the change of the wavelength of the interference light, that is, the difference spectrum, it is extremely difficult to detect the slight change only by measuring the normal spectral curve. In other words, even when there is only a slight wavelength change (wavelength shift) and when the peak as the main wavelength is not formed in the transmittance graph of the interference light, it can be detected easily and reliably. Yes, the wavelength change can be converted into spectral intensity, and can be amplified arbitrarily. As a result, it is a very slight change in wavelength, and even when the peak as the main wavelength in the graph is not formed, it can be detected as an amplified spectral intensity, is highly sensitive, simple and Rapid and highly sensitive detection can be performed.
Furthermore, the target can be quantified by measuring the spectral intensity. That is, it shows the relationship between the amount of transmitted light (the intensity of transmitted light) of the interference light and the wavelength intensity in the difference spectrum of the wavelength change (peak shift) of the interference light by the optical interference means (the target detection substrate). A calibration curve is created in advance, and a calibration curve indicating the relationship between the wavelength intensity of the difference spectrum and the amount of the target captured by the optical interference means (the target detection substrate) is created in advance. If the wavelength intensity of the difference spectrum of the wavelength change (peak shift) of the interference light is measured when the target content of the sample containing the target is measured, the optical interference means can be calculated from the calibration curve. The amount of the target captured by (the target detection substrate) can be quantified.
The above is the wavelength change detection step in the target detection method of the present invention. The wavelength change detection step is a step of detecting a wavelength change of the interference light.

  In the target detection method of the present invention, by using the target detection substrate as the light interference means, the thickness of the surface target interaction is increased by the interaction between the target and the target interaction layer. It is advantageous in that it can emit interference light having a very sharp spectral curve, and can be detected easily and reliably, quickly and with extremely high sensitivity. Further, the target detection method of the present invention can be used in various fields, and can detect various targets such as pathogenic substances, biological substances, toxic substances, etc. efficiently, reliably and simply, and further quantification thereof. And can be suitably used as a diagnostic method, analytical method, quantitative method, and the like.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

Example 1
-Synthesis of filamentous organic matter-
As the filamentous organic molecule, a linear polymer represented by the following structural formula (1) was synthesized by the following method. That is, 0.15 g of acid chlorinated bithione dissolved in DMAc was added dropwise to 1.00 g of polyethyleneimine (number average molecular weight 10,000), reacted, precipitated with 1-octanol, and further washed with diethyl ether. can do.
When the molecular weight and degree of polymerization of the synthesized linear polymer were measured, the number average molecular weight (Mn) was 19,000 and the degree of polymerization was 230. In the following structural formula (1), m: n is 30: 200, R is bitione as the labeled capturing body, and X is
-CO-.

-Manufacture of target detection substrate (light interference means)-
A 50 mm × 50 mm glass substrate (manufactured by SCHOT DESAGAG, B270-SUPERWITE (white plate glass)) as a base material and Ta ₂ O ₅ as a target, and Ta on the glass substrate by an ion assist method. _A substrate was prepared by forming one ₂ O ₅ optical interference layer. On the formed light interference layer, one end of the synthesized linear polymer was bonded (adhered) as the target interaction layer by the following method to produce a target detection substrate A. The surface area of the target detection substrate A is 1.5 × 10 ¹⁴ nm ² (10 mm × 15 mm), and the thickness (Y) of the target interaction layer in the target detection substrate A is a film thickness measurement. The thickness (Y) was 0.26 μm as measured with an apparatus (ULVAC, DEKTAK6M).

-Interaction between optical interference means and target-
The optical interference means is immersed in an aqueous solution of avidin (1.5 μM) as the target for 0.5 hour, and the avidin interacts with biotin as the target capturing body in the optical interference means (adsorption reaction). ) Thereafter, the optical interference means was washed with pure water and dried. After drying, when the thickness (X) of the target interaction layer in the target detection substrate A was measured in the same manner as when the thickness (Y) was measured, the thickness (X) was 0.55 μm. there were. As a result, the increased thickness calculated from the thicknesses (X) and (Y) was 0.29 μm, and the increase rate [(X−Y / Y) × 100 was 112%.
The cross-sectional area of the avidin is about 30 nm ² slightly (3 nm × 3 nm × 3.14).

<Detection of wavelength change and measurement of target by difference spectrum measurement>
The spectrum of interference light (thick line in FIG. 4) by the light interference means (the target detection substrate A) and the spectrum of interference light when the target (avidin) was captured (thin line in FIG. 4) were measured. . The results are shown in FIG. As is apparent from the spectral data of FIG. 4, the two spectral curves almost coincide with each other, and it is difficult to detect the wavelength shift of the interference light from the data of the spectral curves. However, when the difference spectrum of the two spectrum data is taken by the spectrophotometer, it becomes as shown in FIG. 5, and the wavelength difference between the two spectra, which was extremely difficult to detect in FIG. appear. For this reason, by detecting the difference spectrum, the interference light can be detected easily, quickly and with high sensitivity without any measurement error. Since this difference spectrum is obtained as spectral intensity, it can be arbitrarily amplified by the spectrophotometer. That is, even an extremely small spectral intensity can be detected with high sensitivity by amplifying it.

(Example 2)
-Synthesis of helical polymers-
An α-helix polypeptide synthesized by the following method was synthesized as the helical polymer. That is, γ-benzyl-L-glutamic acid was polymerized with cyclic anhydride using triphosgene, and further polymerized using n-hexylamine as an initiator, followed by hydrolysis with potassium hydroxide. This was reacted with acid chlorinated bithione, then precipitated with 1-octanol, and synthesized by washing with diethyl ether.

Next, α-felix polypeptide having biotin in the side chain as the label interaction layer was bound (adhered) to the substrate prepared in Example 1 by the following method to produce a label detection substrate B. .
When the molecular weight and the degree of polymerization of the synthesized α-felix polypeptide were measured, the number average molecular weight was 12,000 and the degree of polymerization was 30.

  When the thickness (Y) of the target interaction layer in the target detection substrate B was measured with a film thickness measuring device (DEKTAK6M manufactured by ULVAC, Inc.), the thickness (Y) was 0.11 μm, and the target The thickness (X) of the interaction layer was 0.19 μm. Therefore, the increased thickness calculated from the thicknesses (X) and (Y) was 0.08 μm, and the increase rate [(XY / Y) × 100 was 73%.

(Comparative Example 1)
In Example 1, a solution obtained by dissolving 80 mg of the synthesized linear polymer in 2.0 g of methanol as a solvent was applied onto the substrate by a spin coating method and dried to form the target interaction layer. A substrate D for target detection was produced.

(Comparative Example 2)
In Comparative Example 1, the substrate E for target detection was produced in place of the α-helix polypeptide synthesized in Example 2 in the linear polymer.
In addition, when the thickness (Y) of the target interaction in the base material for target detection E was measured with a film thickness measuring device (manufactured by ULVAC, DEKTAK6M), the thickness (Y) was 0.11 μm, The target interaction layer had a thickness (X) of 0.13 μm. Therefore, the increased thickness calculated from the thicknesses (X) and (Y) was 0.02 μm, and the rate of increase [(XY) / Y] was 18%.

The target detection apparatus of the present invention can be used in various fields, and can detect various targets such as pathogenic substances, biological substances, toxic substances, etc. efficiently and reliably and simply. Moreover, since the optical interference means has heat resistance, it can be suitably used as a diagnostic device, an analytical device, a quantitative device, etc. used under high temperature conditions.
The target detection method of the present invention can be used in various fields, and can detect various targets such as pathogenic substances, biological substances, and toxic substances efficiently and reliably and simply. In addition, since the optical interference means has heat resistance, it can be suitably used as a diagnostic method, analysis method, quantitative method, etc. under high temperature conditions.

FIG. 1 is a schematic diagram for explaining structural color development (generation of interference color) by a monomolecular film (film-like material) by rod-like organic molecules (rod-like material) provided on a substrate. FIG. 2 is a schematic diagram for explaining the principle of structural color development. FIG. 3 is a schematic view showing an example of the label detection substrate of the present invention. FIG. 4 is a graph showing the spectrum of interference light by the light interference means (target detection substrate) of the present invention and the spectrum of interference light when the target (avidin) is captured. FIG. 5 is a graph showing the difference spectrum when the difference spectrum of the two spectrum data in FIG. 4 is taken.

Explanation of symbols

10 Filamentous organic molecules (filamentous bodies)
10a Target capturing body 20 Target 40 Optical interference layer 50 Base material

Claims (9)

Emits illumination light as interference light with respect to wavelength before of the interference light that interacts with a target, the wavelength of the interference light after interaction with the target was a target detection substrate possible variations And
Target detection substrate, and at least have a can interact target interaction layer and target, and,
The thickness of the target interaction layer after the target detection substrate contacts with the liquid and interacts with the target is larger than the thickness of the target interaction layer before contact with the liquid A substrate for target detection characterized by the above.   The target interaction layer includes a filament having a plurality of target interaction parts capable of interacting with the target, and the filament is erected in contact with a liquid material, and in this state, the target interaction in the filament The target detection substrate according to claim 1, wherein a part interacts with the target.   The thickness (X) of the target interaction layer after the target interaction layer contacts the liquid and interacts with the target, and the thickness (Y) of the target interaction layer before the contact with the liquid The base material for target detection in any one of Claim 1 to 2 which satisfy | fills the relationship of following Formula and [(XY) / Y] * 100> 50%.   The substrate for target detection according to any one of claims 1 to 3, wherein one end of the filamentous body is adhered to a surface of a layer adjacent to the target interaction layer.   The substrate for target detection according to any one of claims 2 to 4, wherein the filamentous body is a filamentous organic molecule.   The substrate for target detection according to claim 5, wherein the filamentous organic molecule is at least one of a linear polymer and a helical polymer.   The filamentous organic molecule comprises at least one selected from an imine group-containing compound, an amine group-containing compound, a hydroxyl group-containing compound, an amide group-containing compound, and a carboxyl group-containing compound as a monomer unit. A substrate for target detection according to claim 1. A light irradiating means for irradiating light, a light interfering means capable of changing a wavelength of interference light of light irradiated by the light irradiating means by interacting with a target, and the light interference means provided in a path of the interference light A wavelength change detecting means for detecting a wavelength change of the interference light emitted by
The optical interference means is a target detection substrate according to any one of claims 1 to 7, characterized in that target interaction layer in target detection substrate is capable of interacting with the target Target detection device. Light is irradiated to the target detection substrate according to any one of claims 1 to 7, the target detection target interaction layer definitive on the substrate, to interact with the target, the light the irradiated A target detection method comprising: a light irradiation step of emitting as interference light; and a wavelength change detection step of detecting a wavelength change of the interference light.

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