JP4205019B2 - Sealed container and vacuum sample collection container - Google Patents

Description

The present invention relates to a sealed container comprising a container made of a thermoplastic resin and a stopper that does not easily loosen even when combined with the container, and a vacuum sample collection container comprising the sealed container.

Various vacuum specimen collection containers are known in which the inside of a sealed container is in a reduced pressure state. As such a vacuum specimen collection container, for example, a vacuum blood collection tube is most common. Examples of the vacuum blood collection tube include those disclosed in Patent Document 1.

An example of a vacuum blood collection system disclosed in Patent Document 1 is shown in FIGS. This vacuum blood collection system includes a vacuum blood collection container 80 shown in FIG. 21A, a vacuum blood collection holder 83 shown in FIG. 21B, and a vacuum blood collection needle 85 shown in FIG. . The vacuum blood collection container 80 includes a blood collection tube 82 having an opening at one end, and a plug 8 that closes the opening of the blood collection tube 82.
1 and. The plug body 81 is made of an elastic material having needle hole sealing properties and gas barrier properties. The vacuum blood collection holder 83 is configured such that the vacuum blood collection container 80 is inserted from one end opening. A blood collection needle holding hole 84 is provided at the other end of the vacuum blood collection holder 83, and a female screw is formed in the blood collection needle holding hole 84. On the other hand, the vacuum blood collection needle 85 has needle tips 87 and 88 at both ends. A hub 86 having a male thread portion is provided on the needle tip 87 side. The hub 86 is configured to be screwed into and fixed to the blood collection needle holding hole 84 of the vacuum blood collection holder 83.

FIG. 23 is a schematic perspective view for explaining a method of collecting blood using the vacuum blood collection system disclosed in Patent Document 1. The blood collection process will be described with reference to FIGS.
At the time of blood collection, the vacuum blood collection needle 85 is screwed into the blood collection needle holding hole 84 of the vacuum blood collection holder 83. Next, the vacuum blood collection container 80 is inserted into the holder 83, and the needle tip 87 of the blood collection needle 85 is pushed to the extent that it does not penetrate the stopper 81, and the needle tip 87 is once sealed. This is to prevent blood from leaking from the needle tip 87 when the needle tip 88 is inserted into the blood vessel. FIG.
3, the blood sampler holds the entire structure in which the blood collection needle 85, the holder 83, and the blood collection container 80 are connected by hand so as to lie in the direction along the blood vessel axis of the blood sample. The needle tip 88 on the blood vessel piercing side is pierced into the blood vessel. Next, by further pushing the blood collection container 80 into the holder 83, the needle tip 87 penetrates the stopper 81, and blood flows into the blood collection container 80 according to the pressure difference between the blood collection container side and the blood vessel side. When the pressure difference between the two sides disappears, the inflow of blood stops, and in this state, the entire blood collection system is moved to remove the needle tip 88 from the blood vessel.

The blood collection needle 85 is a so-called single blood collection needle, and is used when collecting blood only in one vacuum blood collection container. This single blood collection needle cannot be used when collecting blood in a plurality of blood collection containers. That is, when replacing the blood collection container, the needle tip 88 must remain inserted in the blood vessel.
Blood leaks out. On the other hand, in the multiple blood collection needle 89 having the structure shown in FIG. 22, the elastic sheath 90 is extrapolated to the needle tip 87 on the stopper piercing side, and the needle tip 87 is hermetically covered. In addition, blood leakage can be prevented.
When such a multiple blood collection needle 89 is used, the multiple blood collection needle 89 and the holder 8 are used.
3 is prepared, and then the needle tip 88 of the multiple blood collection needle 89 is inserted into the blood vessel. Thereafter, the blood collection container 80 is inserted into the holder 83, and the blood collection container 80 and the blood vessel communicate with each other.

Conventionally, glass has been used as the material used for the blood collection tube 82 as shown in FIG. 21 (a). In recent years, instead of glass blood collection tubes, thermoplastics having excellent gas barrier properties such as polyethylene terephthalate. A plastic blood collection tube made of resin is increasingly used.
On the other hand, as an elastic material used for the stopper 81 as shown in FIG. 21 (a), a gas barrier property is required to maintain the degree of decompression inside the blood collection container, and the needle hole after the needle tip is removed. Conventionally, cross-linked isobutylene / isoprene rubber (cross-linked IIR, cross-linked butyl rubber) has been used because of its sealing ability. However, the cross-linked rubber requires a long time for the vulcanization reaction step, and further requires post-processing such as washing and removing an eluent substance composed of a vulcanization reaction agent or a by-product of the vulcanization reaction, There was a problem of poor productivity. Therefore, in recent years, plugs using injection-moldable thermoplastic resins and thermoplastic elastomers have been disclosed in, for example, Patent Documents 2-7.
Has been proposed.

After blood collection using the vacuum blood collection system shown in FIG. 21, for example, if a biochemical test is performed, serum is obtained as a supernatant after centrifugation after waiting for the completion of blood coagulation. The body 81 is removed, a part of serum is collected using a pipette or the like, and concentration analysis of various components such as electrolytes, enzymes, and lipids is performed using an analytical instrument. The remaining specimen is plug 81 for retesting.
The blood collection container is sealed again and stored refrigerated or frozen. However, for testing, once the blood collection container is opened and the sample is collected and then resealed, the air inside the container is compressed and the internal pressure increases due to the excellent sealing performance of the stopper, and gradually increases. There was a problem that a so-called pop-up phenomenon that the plug body was pushed up and eventually dropped out often occurred.

On the other hand, in Patent Document 8, an annular rib protruding on the inner wall surface or the outer wall surface in the vicinity of the opening end of a glass or plastic blood collection tube is provided, and an annular groove that engages with the annular rib is provided on the rubber stopper. A bottle stopper is disclosed which comprises a provided mating structure. Patent Document 10 discloses a cylindrical case opening and closing device in which a screw cap and a rubber plug are combined.
These fitting structures or screw cap structures are effective means for preventing the pop-up phenomenon. However, in the case of a plastic blood collection tube, the protruding dimension of the annular rib that can be provided is a mold release from the mold unless a complicated slide mechanism is incorporated in the mold. It can only be as small as it does not impair the properties, and it cannot exhibit a clear fit effect. In addition, the screw cap method is complicated to attach and detach, and it takes too much labor to repeatedly open and close a large number of blood collection tubes. In addition, since polyethylene terephthalate, which is often used in plastic blood collection tubes in recent years, has a relatively low heat deformation temperature of about 65 ° C, the inner diameter of the blood collection tube opening portion loses its fitting force with the stopper and increases over time. There was also a problem that the pop-up phenomenon was easy to occur.

On the other hand, when a thermoplastic resin or a thermoplastic elastomer is used for the plug, the thermoplastic resin or the thermoplastic elastomer has a much larger permanent compression set than the thermosetting elastomer. This means that as time passes after being attached to the blood collection tube, especially when left at high temperatures in summer, the fitting force with the blood collection tube is almost lost within a very short time. . Therefore, when a polyethylene resin terephthalate blood collection tube is combined with a stopper made of thermoplastic resin or thermoplastic elastomer, the deformation of the blood collection tube and the loosening due to compression set of the stopper are combined to cause further remarkable loosening. was there.

JP-A-62-227316 JP 57-59536 A Japanese Patent Laid-Open No. 3-97450 JP-A-4-279152 JP 7-51253 A Japanese Patent Laid-Open No. 10-201742 JP 11-318868 A JP 58-142256 A JP-A-52-112481 JP-A-3-505320

In view of the above situation, the present invention provides a sealed container composed of a thermoplastic resin container and a stopper that does not easily loosen even when combined with the container, and a vacuum sample collection container composed of the sealed container. With the goal.

The first aspect of the present invention is a container made of a thermoplastic resin having one end closed and the other end opened;
A sealed container comprising a plug body that can detachably seal one end of the container, the plug body having a grippable head and a bottom end of the container that hangs down from the head. A foot part A that can exert a fitting force on the inner wall surface along the inner wall surface, and a foot part B that can be exerted on the outer wall surface along the outer wall surface of one end of the container that hangs down from the head. A portion of at least the foot B of the plug contacting the container, and at least the foot A of the plug of the container
The part which touches is a sealed container whose load deflection temperature under the load of 0.45 Mpa or 0.46 Mpa is 60 degreeC or more.
The second aspect of the present invention is a container made of a thermoplastic resin having one end closed and the other end opened;
A sealed container comprising a plug body that can detachably seal one end of the container, the plug body having a grippable head and a bottom end of the container that hangs down from the head. A foot part A that can exert a fitting force on the inner wall surface along the inner wall surface, and a foot part B that can be exerted on the outer wall surface along the outer wall surface of one end of the container that hangs down from the head. And the deflection temperature under load of 0.45 Mpa or 0.46 Mpa of the portion of at least the foot B of the plug that contacts the container is the portion of the container at which the foot A of the plug contacts Of 0.4
It is a sealed container having a temperature higher than the deflection temperature under a load of 5 Mpa or 0.46 Mpa.
The present invention is described in detail below.

Hereinafter, the present invention will be described in detail based on an embodiment shown in the accompanying drawings, but the present invention is not limited to the embodiment.
FIG. 1 is a partially cutaway half sectional view showing an embodiment of a sealed container of the present invention. A sealed container 1 shown in FIG. 1 has a stopper 10 fitted to a container 2. FIG. 2 is a partially cut-out half sectional view of the stopper 10, and FIG. 3 is a partially cut-out half sectional view of the container 2.

The container 2 is closed at one end and opened at the other end (open end 3).
The plug body 10 is fitted to the inner wall surface 4 along the inner wall surface 4 of the opening end 3 of the container 2 that hangs down from the head 20 that can be gripped, the partition wall 50 that crosses the opening end 3 of the container 2, and the head 20. Foot A3 that can exert a resultant force
0 and drooping from the head 20 along the outer wall surface 5 of the opening end 3 of the container 2, and near the opening end side 41.
The leg B40 that can exert a fitting force with the outer wall surface 5 is provided.
In the present specification, being able to exert a fitting force means that sliding resistance is accompanied with the attachment / detachment of the plug.

When the stopper 10 is fitted to the container 2 in the sealed container 1 having such a structure, the stopper 10
The outer diameter of the foot portion A30 is subjected to a force that narrows, and the inner diameter of the container 2 is subjected to a force that expands.
On the other hand, when the inner diameter of the container 2 is to be increased, the container 2 is strongly subjected to the fitting force by the foot part B40 of the plug body 10.

In 1st this invention, 0.45 Mpa or 0.46 Mpa of the site | part which contact | connects at least the container 2 of the foot part B40 of the plug body 10, and the at least foot part A30 of the plug body 10 of the container 2
The deflection temperature under load is 60 ° C. or higher. When the deflection temperature under load of these parts is less than 60 ° C., the sealed body 1 is stored for a long time when the temperature in summer is high.
The creep speed of the zero material is remarkably increased, and the fitting force with the container 2 is lost within a short period of time, so that it becomes easy to loosen. More preferably, it is 70 degreeC or more.

In the second aspect of the present invention, at least 0.45M of the portion of the foot B40 of the plug body 10 that is in contact with the container 2
Deflection temperature under load of pa or 0.46 Mpa is at least plug 1 of container 2
It is higher than the deflection temperature under the load of 0.45 Mpa or 0.46 Mpa at the site where the 0 foot A30 is in contact. The plug body 10 is configured so that the load deformation degree of at least the portion of the foot B40 of the plug body 10 that contacts the outer wall surface 5 of the container 2 is equal to or less than the load deformation degree of the portion of the container 2 that contacts the foot portion A30. If the combination of the material and the material constituting the container 2 is selected, the inner diameter of the opening end of the container 2 is prevented from expanding due to the fitting force from the outside of the container 2 of the foot B40 of the plug, so the plug 10 can be prevented from coming off due to looseness.

The load deformation degree can be evaluated by any one of a deflection temperature under load, a Vicat softening point, and a tensile creep elongation.
The load deflection temperature (also referred to as heat distortion temperature) is, for example, ISO 75, ISO 1873.
, ASTM D648, JIS K 6921, JIS K 7191 and the like.
The Vicat softening point is, for example, ISO 306, ASTM D648, ASTM D1
525, measured according to the method defined in JIS K 7206 and the like.
The tensile creep elongation is measured according to a method defined in JIS K 7115.
When the weighted deflection temperature or the Vicat softening point is evaluated as an index, it is determined that the lower the temperature when a certain deformation amount or penetration amount is reached under a certain load, the lower the heat resistance. When the tensile creep elongation is evaluated as an index, it is determined that the heat resistance is lower when the change in the length of the test piece at a certain temperature, load, and loading time is larger.
Of these, the test method that best reflects the phenomenon of loose fitting of the plug body is tensile creep elongation, but since it is easy to measure, it is defined by the deflection temperature under load in the present invention.

In addition, when measuring the deflection temperature under load according to the above standards, it is supposed to use a test piece of a predetermined shape instead of evaluating with a product shape such as an actual plug. Since the crystallinity affects the measured value, it is necessary to measure in a state close to the crystallinity of the actual plug molded product.

In the sealed container 1 which is one of the embodiments of the second aspect of the present invention, the distance at which the foot part B40 of the plug 10 contacts the outer wall surface 5 of the container 2 is such that the foot part A30 contacts the inner wall surface 4 of the container 2. It is preferably shorter in the longitudinal direction of the container 2 than the distance. When the distance at which the foot part B40 of the plug 10 is in contact with the outer wall surface of the container 2 is shorter in the longitudinal direction of the container body 2 than the distance at which the foot part A30 is in contact with the inner wall surface of the container 2, The inner diameter of the container at the part not receiving the fitting force of the foot part B40 is caused to creep by the fitting force from the inside of the foot part A30 to increase.
As a result, since the container is deformed in the shape of a stenosis, it is possible to more effectively prevent the stopper 10 from coming off due to looseness. Therefore, when blood is leaked when the sealed container 1 is laid down or upside down after blood collection, or when the plug 10 is mounted again after opening and collecting a part of the sample, the plug 10 The problem that the function as a sealed container is impaired, such as gradually floating and eventually falling off, is unlikely to occur.

In the sealed container 1 which is one of the embodiments of the second aspect of the present invention, when the entire foot B40 of the stopper 10 is in contact with the outer wall surface 5 of the container 2, when the stopper 10 is repeatedly attached and detached after blood collection, The blood in the container soaks into the gap between the foot B40 and the outer wall surface 5 of the container 2 and easily contaminates the operator's fingers. Therefore, it is more preferable that the foot B40 has an end portion that is along the outer wall surface 5 of the container 2 but is not in contact with the outer wall surface 5 of the container 2.

In the sealed container 1, in the first aspect of the present invention, materials constituting the container 2 and the plug body 10 are selected so as to satisfy the above-described deflection temperature under load. In the second aspect of the present invention, a combination of materials constituting the container 2 and the plug body 10 is selected so that the deflection temperature under load between the container 2 and the plug body 10 satisfies the above relationship.
The container 2 is made of a thermoplastic resin. The thermoplastic resin constituting the container 2 is not particularly limited. For example, a chain or cyclic olefin resin, a styrene resin, an acrylic resin, an acrylonitrile resin, an acrylonitrile / styrene resin, an acrylonitrile / styrene /
Examples thereof include butadiene resins, ester resins, amide resins, and modified cellulose resins.
The material constituting the plug 10 is not particularly limited. For example, in addition to the thermoplastic resin exemplified as the material of the container, a phenol resin, an unsaturated ester resin, a urea resin,
Examples thereof include thermosetting resins such as melamine resins; metals such as aluminum and stainless steel; ceramics and the like.

The method for manufacturing the plug body 10 is not particularly limited. For example, the plug body 10 may be cut or integrally formed with the head 20 and the foot B40 by an extrusion compression molding method, an injection molding method, or the like. For example, a method of inserting the foot part A30 is used. Of course, the head 20, the foot B40, and the foot A30 may be integrally formed.
It does not specifically limit as a method of manufacturing the said container 2, A conventionally well-known injection molding method etc. can be used.
By fitting the plug 10 into the opening of the container 2 thus manufactured, the sealed container 1
Can be manufactured.

FIG. 4 is a partially cut-away half sectional view of a sealed container 1a derived from the embodiment shown in FIG. In the sealed container 1a, the stopper 10a is fitted to the container 2a. FIG. 5 shows the plug 10a.
FIG. 6 is a partially cutaway half sectional view of the container 2a.
In the sealed container 1a, the foot B40 hanging from the head 20 of the stopper 10a is along the outer wall surface 5 of the opening end of the container 2a, and the opening end side vicinity 41 can be fitted to the outer wall surface 5. Is container 2a
This is because an annular rib 5a protruding outward is provided at the open end 3 of the first. Other configurations of the container and the plug are the same as those in the embodiment shown in FIG.

FIG. 7 is a partially cut-out half sectional view of a sealed container 1b which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1b, the stopper 10b is fitted to the container 2. FIG.
These are the partial notch half sectional views of the plug 10b.
In the sealed container 1b, the position where the fitting force between the foot part A30 of the plug 10b and the inner wall surface 4 of the container 2 is maximized, and the position between the foot B40 of the plug body 10b and the outer wall surface 5 of the container 2 are used. The position at which the fitting force is maximum is at a position different in the longitudinal direction of the container 2. That is, the maximum fitting force between the foot part A30 hanging from the head 20 of the plug 10b and the inner wall surface 4 of the container 2 is larger than that of the fitting part 41 between the foot part B40 and the outer wall surface 5 of the container 2. 2, the inner diameter of the container 2 and the outer diameter of the foot A30 are determined so as to act on the closed end side, and the foot A30 projects toward the inner wall surface 4 side of the container 2. An annular rib 31 is provided. Such an annular rib 31 does not need to be provided in a limited manner on the foot A30 of the stopper 10b, and is provided to protrude toward the foot A30 at a position opposite to the inner wall surface 4 of the container 2. Also good. Further, the height and number of the annular ribs 31 are not particularly limited as long as there is no problem when the plug body is molded by the injection molding method.
By providing such an annular rib 31, the container 2 is easily deformed into a constricted shape, so that even if the sealed container 1b is stored at a high temperature for a long time, blood leaks after blood collection,
There is no possibility that the function as the sealed container is impaired, for example, when the plug 10b is lifted from the sealed container 1b.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

FIG. 9 is a partially cut-out half sectional view showing a sealed container 1c which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1c, the stopper 10c is fitted to the container 2.
FIG. 10 is a partially cut-out half sectional view of the plug 10c.
In the sealed container 1c, a surface layer 32 made of a thermoplastic elastomer or a thermosetting elastomer is formed at least on a part of the foot A30 that hangs down from the head part 20 of the plug 10c in contact with the inner wall surface 4 of the container 2. . By having such a surface layer 32, the sealing performance can be further enhanced.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

It does not specifically limit as a thermoplastic elastomer which comprises the said surface layer 32, For example, an olefin type, a styrene type, ester type, an amide type, a urethane type etc. are mentioned. These thermoplastic elastomers may be used alone or in combination of two or more.
The thermosetting elastomer constituting the surface layer 32 is not particularly limited, and examples thereof include natural rubber, isoprene rubber, isobutylene / isoprene rubber, styrene / butadiene rubber, neoprene rubber, and silicon rubber. It is done. These thermosetting elastomers may be used alone or in combination of two or more.
Moreover, in order to improve airtightness as a sealed container regardless of thermoplastic elastomer or thermosetting elastomer, it is preferably 10 times or less, more preferably 6 times or less, more preferably 3 times that of isobutylene / isoprene vulcanized rubber. A material having an oxygen permeability coefficient (25 ° C.) of not more than double and excellent gas barrier properties is suitable.
The surface layer 32 may be embedded by injection molding or compression vulcanization, or may be integrally formed by an insert molding method.

On the surface of the surface layer 32, various oils, waxes, fatty acid / fatty acid salts, fatty acid amides, surfactants, plasticizers, in order to reduce frictional resistance when fitting the plug 10 c to the container 2,
Lubricants such as lubricating inorganic fine powder or lubricating organic fine powder may be applied. Further, these lubricants may be blended in the thermoplastic elastomer or thermosetting elastomer that constitutes the surface layer 32 in advance.

The thermoplastic elastomer or the thermosetting elastomer constituting the surface layer 32 includes an ethylene-vinyl acetate copolymer, an ethylene-acrylic acid copolymer, an ethylene-methacrylic acid copolymer, an ethylene-acrylic acid ester copolymer in advance. Polymers, ethylene-methacrylic acid ester copolymers, and polyethylene, polypropylene, etc. having a high MFR value of preferably 30 or more, more preferably 50 or more, and even more preferably 70 or more are blended as fluidity improvers during molding. May be. In this case, the blending amount of the fluidity improver is preferably 20% by weight or less. If it exceeds 20% by weight, the elastic properties of the elastomer may be adversely affected. More preferably, it is 10 weight% or less, More preferably, it is 5 weight% or less.

The surface layer 32 preferably has a JIS hardness A or ASTM Shore hardness A of 80 or less. If it exceeds 80, the resistance at the time of fitting the plug 10c to the container 2 increases, or the container 2
Adhesiveness to the inner surface may be reduced, and workability and airtightness may be inferior.
More preferably, it is 60 or less.

FIG. 11 is a partially cut-out half sectional view showing a sealed container 1d which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1d, a stopper 10d is fitted to the container 2. FIG. 12 is a partially cut-out half sectional view of the plug 10d.
In the sealed container 1d, a needle tube insertable portion 60 made of a thermoplastic elastomer or a thermosetting elastomer is provided at the center of the partition wall portion 50 of the stopper 10d. By having such a needle tube insertion-possible part 60, without removing the plug 10d from the container 2, the needle tube is inserted into the sealed container to inject blood or to collect blood. Can be.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

As the thermoplastic elastomer or thermosetting elastomer constituting the needle tube insertable portion 60, the same ones as described above can be used. Moreover, in order to incorporate these thermoplastic elastomers or thermosetting elastomers into the partition wall portion 50, a conventionally known method such as fitting a pre-formed one or integrally forming by an insert molding method can be used.

The needle tube insertable portion 60 preferably has a JIS hardness A or an ASTM Shore hardness A of 80 or less. If it exceeds 80, the needle tube piercing resistance may increase or the needle hole sealability may decrease. More preferably, it is 60 or less.

FIG. 13 is a partially cutaway half sectional view showing a sealed container 1e which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1e, the stopper 10e is fitted to the container 2. FIG. 14 is a partially cut-out half sectional view of the plug 10e.
In the sealed container 1e, an opening 35 for introducing a needle tube is provided at the center of the partition wall 51 of the stopper 10e, and a foot A30 that hangs down from the head 20 and fits to the inner wall surface of the container 2 is provided. Is made of a thermoplastic elastomer or a thermosetting elastomer that can be inserted into a needle tube. The opening 35 for introducing the needle tube is not necessarily provided at the center, and the number of the opening 35 is not limited to one, and a plurality of openings 35 may be provided.
As the thermoplastic elastomer or thermosetting elastomer, the same ones as described above can be used. Moreover, it does not specifically limit as a manufacturing method of foot part A30, You may insert-mold foot part A30 in the plug body head 20 shape | molded previously, or foot part A shape | molded separately.
30 may be adhered.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

FIG. 15 is a partially cut-out half sectional view showing a sealed container 1f which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1f, the stopper 10f is fitted to the container 2. FIG. 16 is a partially cut-out half sectional view of the plug 10f.
In the sealed container 1f, an opening 35 for introducing a needle tube is provided at the center of the partition wall 51 of the stopper 10f, and a foot A30 that hangs down from the head 20 and fits to the inner wall surface of the container 2 is provided. Is made of a thermoplastic elastomer or thermosetting elastomer that can be inserted into a needle tube, and further includes a head 20.
The auxiliary foot portion 70 for reinforcing the fitting force of the foot portion A30 and reinforcing the adhesive strength or fusion strength with the head portion 20 hangs down.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

FIG. 17 is a partially cut-out half sectional view showing a sealed container 1g which is another preferred embodiment of the sealed container of the present invention. In the sealed container 1g, the stopper 10g is fitted to the container 2. FIG. 18 is a partially cut-out half sectional view of the plug body 10g.
In the sealed container 1g, an opening 35 for introducing a needle tube is provided at the center of the partition wall 51 of the stopper 10g, and a foot A30 that hangs down from the head 20 and fits to the inner wall surface of the container 2 is provided. A foot B40 made of a thermoplastic elastomer or a thermosetting elastomer that can be inserted into a needle tube, and further hangs down from the head 20 toward the bottom side of the container 2 beyond the fitting length of the foot A30.
The foot portion B40 has a portion that is in contact with the outer wall surface 5 of the container 2 in the vicinity of the opening end 41 of the container and exerts a fitting force, and a skirt portion 42 that does not contact the outer wall surface 5 and does not exert the fitting force. Have.
The skirt portion 42 has a function of blocking blood splashes that may scatter when the plug is removed after blood collection to ensure the safety of the operator.
Other configurations of the container and the plug are the same as those of the embodiment shown in FIG.

Since the sealed container of the present invention has the above-described configuration, it is difficult for the stopper to loosen even when combined with a thermoplastic resin container. The sealed container of the present invention has a reduced pressure inside, for example,
It can be suitably used for a vacuum specimen collection container such as a vacuum blood collection tube.
A vacuum sample collection container comprising the sealed container of the present invention and having a reduced pressure inside is also one aspect of the present invention.

ADVANTAGE OF THE INVENTION According to this invention, the sealed container which consists of a container made from a thermoplastic resin, and the stopper which does not produce looseness even if it combines with this container, and the vacuum sample collection container which consists of this sealed container can be provided.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples.

(Examples 1 and 2)
A sealed container having the same configuration as that of the sealed container 1g shown in FIG. 17 was produced.
(1) Production of plug body A plug body was produced using the materials shown in Table 1. The structure and dimensions of the plug were as shown in FIG.
First, high impact polystyrene ("PSJ polystyrene", deflection temperature under load (0.4
5 MPa): about 75 ° C., manufactured by PS Japan) and polypropylene (“Sun Aroma”, deflection temperature under load (0.45 MPa): about 95 ° C., manufactured by Sun Aroma)) Was integrally formed by injection molding.
Next, in accordance with the combinations shown in Table 1, a styrene thermoplastic elastomer (“Lavalon”, JISA hardness: about 50, manufactured by Mitsubishi Chemical Corporation) and an EPDM thermoplastic elastomer (“
"Santoprene", JISA hardness: about 55, manufactured by AES Japan Co., Ltd.) was inserted into a predetermined position of the obtained head and foot B, and the foot A was molded to produce the plug of FIG.
Each thermoplastic elastomer was previously blended with 0.5% by weight of an oleic acid amide-based lubricant.

(2) Preparation of containers and sealed containers Polyethylene terephthalate ("Dianite", deflection temperature under load (0.45 MPa):
Using an inner diameter of 10.7 mm and an outer diameter of 13.2 m.
m, a container with a total length of 100 mm and a capacity of 7 mL was injection molded.
The obtained container was stoppered with a stopper in a reduced pressure state to obtain a sealed container (vacuum blood collection tube) for 6 mL.

(Comparative Examples 1-3)
Using the materials shown in Table 1, a plug was produced in the same manner as in Example 1. The structure and dimensions of the plug were as shown in FIG. The stopper shown in FIG. 20 has a structure in which a portion 41 that exerts a fitting force on the outer wall surface of the container opening end is removed from the stopper shown in FIG.
A sealed container (vacuum blood collection tube) was obtained in the same manner as in Example 1 except that the obtained plug was used.

(Comparative Example 4)
A plug is produced by the same method as in Example 1 except that the materials shown in Table 1 are used, and a sealed container (vacuum blood collection tube) is obtained by the same method as in Example 1 except that the plug is used. It was.

ハイインパクトポリスチレン（「ＰＳＪポリスチレン」、荷重たわみ温度（０．４５ＭＰ
ａ）：約７５℃、ＰＳジャパン社製）
ポリプロピレン（「サンアロマー」、荷重たわみ温度（０．４５ＭＰａ）：約９５℃、サ
ンアロマー社製）
スチレン系熱可塑性エラストマー（「ラバロン」、ＪＩＳＡ硬度：約５０、三菱化学社製
）ＥＰＤＭ系熱可塑性エラストマー（「サントプレン」、ＪＩＳＡ硬度：約５５、ＡＥＳ
ジャパン社製）
ポリエチレンテレフタレート（「ダイヤナイト」、荷重たわみ温度（０．４５ＭＰａ）：
約６９℃、三菱レイヨン社製）

High impact polystyrene ("PSJ polystyrene", deflection temperature under load (0.45MP
a): about 75 ° C., manufactured by PS Japan Ltd.)
Polypropylene (“Sun Aroma”, deflection temperature under load (0.45 MPa): about 95 ° C., manufactured by Sun Aroma)
Styrenic thermoplastic elastomer ("Lavalon", JISA hardness: about 50, manufactured by Mitsubishi Chemical) EPDM thermoplastic elastomer ("Santoprene", JISA hardness: about 55, AES
(Made by Japan)
Polyethylene terephthalate ("Dianite", deflection temperature under load (0.45 MPa):
About 69 ° C, manufactured by Mitsubishi Rayon Co., Ltd.)

(Evaluation)
For the sealed containers produced in Examples 1 and 2 and Comparative Examples 1 to 4, water was vacuum collected immediately after production, and the pulling resistance was measured as an index of the fitting force of the stopper. Moreover, after opening once and collecting 1 mL of contents water, it sealed again and immediately measured the re-drawing resistance. Then, it was sealed again and left at room temperature of about 25 ° C. for 10 minutes to observe whether or not the plug gradually floated.
Further, the same evaluation was performed after the heat aging at 60 ° C. for 1 week after the production.
In addition, using an Instron type material testing machine, the force required to pull out the plug straight at a speed of 200 mm / min was measured, and this was taken as the pulling resistance.
The results are shown in Table 2.

引抜抵抗は、実施例で作製した密封容器、比較例で作製した密封容器を問わず、加熱経変
後もほぼ同水準の嵌合力を示しているように見える。しかし、これは足部の熱可塑性エラ
ストマーがポリエチレンテレフタレート製採血管の内壁面に経時的に粘着したためであっ
た。一方、再引抜抵抗については、実施例で作製した密封容器は比較例で作製した密封容
器に比べて明らかに大きな値を示し、両者の違いは顕著であった。この結果、実施例で作
製した密封容器では栓体浮きも観察されず、優れた密封性を維持していた。

The pull-out resistance seems to show almost the same level of fitting force even after heating, regardless of the sealed container produced in the example and the sealed container produced in the comparative example. However, this was because the thermoplastic elastomer at the foot adhered to the inner wall surface of the polyethylene terephthalate blood collection tube over time. On the other hand, regarding the redraw resistance, the sealed container produced in the example showed a clearly larger value than the sealed container produced in the comparative example, and the difference between the two was remarkable. As a result, in the sealed container produced in the example, no stopper floating was observed, and excellent sealing performance was maintained.

(Examples 3 to 7)
A sealed container having the same configuration as that of the sealed container 1g shown in FIG. 17 was produced.
(1) Production of plug body A plug body was produced by the two-material injection molding method using the materials shown in Table 3. The structure and dimensions of the plug were as shown in FIG.
Each thermoplastic elastomer was previously blended with 0.5% by weight of an oleic acid amide-based lubricant.
(2) Preparation of container and sealed container Using the materials shown in Table 3, the inner diameter of the open end is 10.7 mm, the outer diameter is 13.2 mm, the total length is 10
A container of 0 mm and 7 mL capacity was injection molded.
The obtained container was stoppered with a stopper in a reduced pressure state to obtain a sealed container (vacuum blood collection tube) for 6 mL.

(Comparative Examples 5-9)
(1) Production of plug body A plug body was produced by the two-material injection molding method using the materials shown in Table 3. The structure and dimensions of the plug were as shown in FIG.
Each thermoplastic elastomer was previously mixed with 0.5% by weight of an oleic acid amide-based lubricant.
(2) Preparation of container and sealed container Using the materials shown in Table 3, the inner diameter of the open end is 10.7 mm, the outer diameter is 13.2 mm, the total length is 10
A container of 0 mm and 7 mL capacity was injection molded.
The obtained container was stoppered with a stopper in a reduced pressure state to obtain a sealed container (vacuum blood collection tube) for 6 mL.

エチレンプロピレンランダム共重合体（「サンアロマー」、荷重たわみ温度（０．４５Ｍ
Ｐａ）：約７０℃、サンアロマー社製）
ハイインパクトポリスチレン（「ＰＳＪポリスチレン」、荷重たわみ温度（０．４５ＭＰ
ａ）：約７５℃、ＰＳジャパン社製）
ポリプロピレン（「サンアロマー」、荷重たわみ温度（０．４５ＭＰａ）：約９５℃、サ
ンアロマー社製）
スチレン系熱可塑性エラストマー（「ラバロン」、ＪＩＳＡ硬度：約５０、三菱化学社製
）
低密度ポリエチレン（「ＮＵＣ高圧法ポリエチレン」、荷重たわみ温度（０．４５ＭＰａ
）：約４５℃、日本ユニカー社製）
アクリロニトリル（「バレックス」、荷重たわみ温度（０．４５ＭＰａ）：約７４℃、三
井化学製）
ポリメチルペンテン（「TPX」、荷重たわみ温度（０．４５ＭＰａ）：約８０℃、三井化
学製）

Ethylene propylene random copolymer ("Sun Aroma", deflection temperature under load (0.45M
Pa): about 70 ° C., manufactured by Sun Allomer Co.)
High impact polystyrene ("PSJ polystyrene", deflection temperature under load (0.45MP
a): about 75 ° C., manufactured by PS Japan Ltd.)
Polypropylene (“Sun Aroma”, deflection temperature under load (0.45 MPa): about 95 ° C., manufactured by Sun Aroma)
Styrenic thermoplastic elastomer ("Lavalon", JISA hardness: approx. 50, manufactured by Mitsubishi Chemical Corporation)
Low density polyethylene ("NUC high pressure polyethylene", deflection temperature under load (0.45 MPa
): Approximately 45 ° C, manufactured by Nippon Unicar Co., Ltd.)
Acrylonitrile (“Barex”, deflection temperature under load (0.45 MPa): about 74 ° C., manufactured by Mitsui Chemicals)
Polymethylpentene (“TPX”, deflection temperature under load (0.45 MPa): about 80 ° C., manufactured by Mitsui Chemicals)

(Evaluation)
For the sealed containers produced in Examples 3-7 and Comparative Examples 5-9, immediately after production and at 60 ° C.
The drawing resistance was measured by the same method as in Example 1 after heating for 1 week.
The results are shown in Table 4.

（実施例８、９、比較例１０）
図７に示した密封容器１ｂと同様の構成を有する密封容器を作製した。
（１）栓体の作製
表５に示した材料を用いて二材射出成型法により栓体を作製した。なお、栓体の構造及び
寸法は図２４に示す通りとした。
なお、それぞれの熱可塑性エラストマーには、予めオレイン酸アミド系の滑剤０．５重量
％を配合した。
（２）容器及び密封容器の作製
表５に示した材料を用いて、開口端部の内径１０．７ｍｍ、外径１３．２ｍｍ、全長１０
０ｍｍ、容量７ｍＬの容器を射出成型した。
得られた容器に減圧状態で栓体を打栓して、６ｍＬ用の密封容器（真空採血管）を得た。

(Examples 8 and 9, Comparative Example 10)
A sealed container having the same configuration as the sealed container 1b shown in FIG. 7 was produced.
(1) Production of plug body A plug body was produced by the two-material injection molding method using the materials shown in Table 5. The structure and dimensions of the plug were as shown in FIG.
Each thermoplastic elastomer was previously blended with 0.5% by weight of an oleic acid amide-based lubricant.
(2) Production of container and sealed container Using the materials shown in Table 5, the inner diameter of the open end is 10.7 mm, the outer diameter is 13.2 mm, the total length is 10
A container of 0 mm and 7 mL capacity was injection molded.
The obtained container was stoppered with a stopper in a reduced pressure state to obtain a sealed container (vacuum blood collection tube) for 6 mL.

高密度ポリエチレン（「NUCポリエチレン」、荷重たわみ温度（０．４５ＭＰａ）：約７
０℃、日本ユニカー社製）
低密度ポリエチレン（「ＮＵＣ高圧法ポリエチレン」、荷重たわみ温度（０．４５ＭＰａ
）：約４５℃、日本ユニカー社製）
ポリプロピレン（「サンアロマー」、荷重たわみ温度（０．４５ＭＰａ）：約９５℃、サ
ンアロマー社製）

High density polyethylene (“NUC polyethylene”, deflection temperature under load (0.45 MPa): about 7
0 ℃, made by Nihon Unicar)
Low density polyethylene ("NUC high pressure polyethylene", deflection temperature under load (0.45 MPa
): Approximately 45 ° C, manufactured by Nippon Unicar Co., Ltd.)
Polypropylene (“Sun Aroma”, deflection temperature under load (0.45 MPa): about 95 ° C., manufactured by Sun Aroma)

(Evaluation)
For the sealed containers produced in Examples 8 and 9 and Comparative Example 10, immediately after production and at 60 ° C., 1
The drawing resistance was measured by the same method as in Example 1 after weekly heating.
The results are shown in Table 6.

引抜抵抗については、実施例で作製した密封容器は比較例で作製した密封容器に比べて明
らかに高い値を示し、優れた嵌合力を維持していた。

Regarding the pull-out resistance, the sealed container produced in the example clearly showed a higher value than the sealed container produced in the comparative example and maintained an excellent fitting force.

ADVANTAGE OF THE INVENTION According to this invention, the sealed container which consists of a container made from a thermoplastic resin, and the stopper which does not produce looseness even if it combines with this container, and the vacuum sample collection container which consists of this sealed container can be provided.

It is a partial notch half sectional view which shows one Embodiment of the sealed container of this invention. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. It is a partial notch half sectional view of the container of the sealed container shown in FIG. It is a partial notch half sectional view of the sealed container 1a derived | led-out from embodiment shown in FIG. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. FIG. 5 is a partially cut-out half sectional view of the container of the sealed container shown in FIG. 4. It is a partial notch half sectional view which shows suitable one Embodiment of the sealed container of this invention. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. It is a partial notch half sectional view which shows other suitable one Embodiment of the sealed container of this invention. FIG. 10 is a partially cut-out half cross-sectional view of the stopper of the sealed container shown in FIG. 9. It is a partial notch half sectional view which shows other suitable one Embodiment of the sealed container of this invention. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. It is a partial notch half sectional view which shows other suitable one Embodiment of the sealed container of this invention. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. It is a partial notch half sectional view which shows other suitable one Embodiment of the sealed container of this invention. It is a partial notch half cross-sectional view of the stopper of the sealed container shown in FIG. It is a partial notch half sectional view which shows other suitable one Embodiment of the sealed container of this invention. It is a partial notch half sectional view of the stopper of the sealed container shown in FIG. It is a figure which shows the structure and dimension of the plug body produced in Example 1,2. It is a figure which shows the structure and dimension of the plug body produced in Comparative Examples 1, 2, and 3. FIG. It is a figure which shows the basic composition of the conventional vacuum blood collection system. It is a side view which shows a multiple blood collection needle. It is a perspective view which shows the state at the time of blood-collecting using a vacuum blood-collection system. It is a figure which shows the structure and dimension of the plug produced in Examples 8 and 9 and Comparative Example 10.

Explanation of symbols

1, 1a, 1b, 1c, 1d, 1e, 1f, 1g Sealed container 2, 2a Container 3 Open end 4 Inner wall surface 5 Outer wall surface 5a Annular rib 10, 10a, 10b, 10c, 10d, 10e, 10f, 10g 20 head 30 foot A
32 Surface layer 35 Vacuum blood collection needle 40 Foot B
41 Open end side vicinity 42 Skirt part 50, 51 Partition part 60 Needle tube insertion possible part 70 Auxiliary foot part 80 Vacuum blood collection container 81 Plug body 82 Blood collection tube 83 Holder 84 Blood collection needle holding hole 85 Vacuum blood collection needle 86 Hub 87, 88 Needle Point 89 Multiple blood collection needle 90 Elastic sheath body

Claims (6)

A sealed container comprising a container made of a thermoplastic resin having one end closed and the other end opened, and a stopper capable of detachably sealing the opened one end of the container,
The plug body includes a grippable head, a foot A that hangs down from the head and extends along an inner wall surface of one end of the container, and that can exert a fitting force on the inner wall surface. And along the outer wall surface of the open end of the container, and has a foot B that can exert a fitting force on the outer wall surface,
The deflection temperature under load of at least 0.45 Mpa or 0.46 Mpa of the part of the plug B in contact with the container is 0.45 Mpa of at least the part of the plug in contact with the foot A of the plug. A sealed container having a temperature higher than a deflection temperature under a load of 0.46 Mpa. 2. The sealed container according to claim 1 , wherein a distance at which the foot B of the plug contacts the outer wall surface of the container is shorter in a longitudinal direction of the container than a distance at which the foot A of the plug contacts the inner wall of the container. . The position where the fitting force between the foot part A of the stopper and the inner wall surface of the container is maximized and the position where the fitting force between the foot part B of the stopper and the outer wall surface of the container are maximized are the container. The sealed container according to claim 1, wherein the container is in a different position in the longitudinal direction. The sealed container according to claim 1, 2 or 3 , wherein the foot part A of the plug has a surface layer made of a thermoplastic elastomer or a thermosetting elastomer at least at a portion in contact with the inner wall surface of the container. The sealed container according to claim 1, 2, 3, or 4 , wherein the stopper has a needle tube insertable portion made of a thermoplastic elastomer or a thermosetting elastomer. 6. A vacuum specimen collection container comprising the sealed container according to claim 1 , wherein the inside thereof is in a reduced pressure state.

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