JP4151812B2 - Pest control method by fake egg transportation - Google Patents
Pest control method by fake egg transportation Download PDFInfo
- Publication number
- JP4151812B2 JP4151812B2 JP19487099A JP19487099A JP4151812B2 JP 4151812 B2 JP4151812 B2 JP 4151812B2 JP 19487099 A JP19487099 A JP 19487099A JP 19487099 A JP19487099 A JP 19487099A JP 4151812 B2 JP4151812 B2 JP 4151812B2
- Authority
- JP
- Japan
- Prior art keywords
- egg
- termite
- sclerotia
- nest
- termites
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Description
【0001】
【発明の属する技術分野】
本発明はシロアリの巣内に活性化合物を効果的に導入する方法に関するものである。
【0002】
【従来の技術及び発明が解決しようとする課題】
シロアリの駆除方法としては溶液型薬剤を侵入箇所に注入して殺虫する方法がある。しかし、この方法は一時的な効果しかなく、木材に十分浸透させるためには多量の薬剤を必要とした。さらに、被害部分だけ駆除を行うとシロアリは通路を変更して他の部分に被害を移すことになり、その駆除は一層困難を極める。頻繁に実施されているのは臭化メチルを用いた燻蒸法である。しかし臭化メチルはオゾン層破壊の原因物質であり、近年使用を規制しようとする動きが強まっている。シロアリと同様に社会生活を営むアリの駆除法としては毒物にアリの嗜好物を混入して餌として与え、巣に持ち帰らせて全集団を捕殺する方法がある。しかしシロアリは営巣している木材自体を摂食するため、毒餌剤を用いて巣の外部から巣の内部に薬剤を運搬させる方法は適用できない。
【0003】
【課題を解決するための手段】
本発明者はシロアリの巣内に効率的に活性化合物を導入する方法について検討した結果、シロアリの卵認識及び卵運搬行動に着目し、卵を模した基材に卵から抽出した卵認識物質を塗布することによって巣内に効率的に運搬させる方法を見出し本発見に至った。シロアリの職蟻は女王が産んだ卵を運搬して山積みにし、卵の表面を舐めるなどして世話をする性質をもつ。なお職蟻はシロアリの卵に似る大きさと形状をもち、表面に卵認識物質を含有する擬似卵を卵として認識し、巣内にある自らの卵塊中に運搬する。この擬似卵に殺虫活性化合物や孵化阻害物質などの活性化合物を含有せしめて卵塊中に運搬させることにより、効率的にコロニーの生殖中枢を破壊することが可能である。また本発明者はヤマトシロアリの卵に擬態した菌核(sclerotia)を形成し、卵塊中に共生する新種のアセリア属菌核菌(Atheria sp.nov.)を発見した。本糸状菌はPDA(potato−dextrose agar)培地上で発育及び菌核形成をさせることが可能であり、培養によって得た菌核に活性化合物及び卵認識物質を含有せしめてシロアリに運搬させることも可能である。
【0004】
【発明の実施の形態】
シロアリの卵以外の物体をシロアリに卵として認識させ、巣内に運搬させるためには、物体を物理的・化学的に卵に似せることが必要である。本発明においては直径0.3〜0.45mmの球形をした成形体又は菌核を基材とし、この表面にシロアリの卵認識物質を含有せしめたものを擬似卵として用いる。実際のシロアリの卵は長卵形であり、長卵形の擬似卵を用いてもよいが、成形するに当たっては球形の方が容易である。
【0005】
シロアリの卵認識物質を基材に含有せしめる方法としては、シロアリの巣から卵塊を採取し、エーテルを溶媒として卵の表面物質を抽出し、これを基材に含浸・塗布する。なおヤマトシロアリの卵から抽出した卵認識物質はイエシロアリに対しても同様の効果をもつ。この擬似卵をシロアリの蟻道内あるいは侵入箇所に注入し、職蟻に運搬させる。一度運搬が開始されると、職蟻が次々に運搬するため効率的に擬似卵を巣の中枢に導入できる。
【0006】
本発明に用いられる擬似卵を作製するに当たり、基材として好適な材料には様々なものがある。例えば、ポリエチレン、ポリプロピレン、ポリスチレン、ポリエステル、ポリ塩化ビニル、ポリカーボネート等の熱可塑性樹脂、あるいは尿素樹脂、エポキシ樹脂、フェノール樹脂、ポリウレタン等の熱硬化性樹脂のほかにも、シリカゲルなどの多孔質ゲル化剤、ガラス等が挙げられる。これらの素材を直径0.3〜0.45mmの球形に成形したものを擬似卵の基材とすることができる。またはシロアリ共生菌核菌を人工培養して得られる菌核(sclerotia)を基材として用いることもできる。好適な素材はこれらの例に限らないが、素材の比重が卵と著しく異なるものは運搬されない。
【0007】
本発明に用いられる擬似卵にはその運搬率を損ねない範囲で活性化合物を含有せしめることができる。例としてはピレスロイド化合物、有機リン化合物、カーバメイト化合物、N−アリールジアゾール化合物、ヒドラゾン化合物、スルホンアミド化合物、天然殺虫成分化合物などの殺虫活性成分のみならず、孵化阻害物質、幼若ホルモン様物質等の昆虫発育制御物質などが挙げられる。擬似卵に活性化合物を含有せしめる方法としては樹脂等に練り込む、あるいは多孔質体に含浸・塗布する等の方法が挙げられる。
【0008】
【実施例】
次に、本発明の擬似卵の製造例及び試験例を示すが、本発明は以下の例のみに限定されるものではない。
【0009】
製造例1(菌核を基材にした擬似卵):ヤマトシロアリの卵6.0重量%にジェチルエーテル94.0重量%を加え、一分間超音波洗浄した。これを濾過して卵を取り除き、卵表面物質の抽出溶液(1)を得た。PDA培地で培養して得たシロアリ共生菌核菌の菌核3.5重量%に卵抽出溶液(1)96.5重量%を加えた後、エーテル溶媒を揮発させて菌核の表面に卵認識物質を付着させる。
【0010】
製造例2(ガラスビーズを基材にした擬似卵):直径0.4mmのガラスビーズ(相互理化学硝子製作所(株)製)12.5重量%に製造例1に記載の卵抽出溶液(1)87.5重量%を加えた後、エーテル溶媒を揮発させてガラスビーズの表面に卵認識物質を付着させる。
【0011】
比較例(海砂を基材にした場合):30meshの海砂(和光純薬(株)製)7.0重量%に製造例1に記載の卵抽出溶液(1)93.0重量%を加えた後、エーテル溶媒を揮発させて海砂の表面に卵認識物質を付着させる。
【0012】
試験例:直径9cmのシャーレ上にシロアリの卵20個と製造例1によって得た擬似卵20個をランダムに撒き、これにシロアリの職蟻30個体を入れて15時間25℃の恒温室内で静置した。職蟻はシャーレ上に散在した卵を集めて卵塊を形成した。15時間後に卵塊を調べたところ、90%以上の擬似卵が卵塊中に運搬されていた。同様の試験法で製造例2及び比較例によって得た擬似
卵の運搬率を調べたところ、製造例2の擬似卵は40%以上運搬されたが、比較例の擬似卵は全く運搬されなかった。また、室内飼育するシロアリのコロニーが形成した蟻道中に製造例1によって得た擬似卵50個を注入したところ、24時間後には擬似卵の80%以上が巣内の卵塊中に運搬された。
【0013】
【発明の効果】
本発明により、害虫駆除の目的で人工物を効率的に害虫の巣内に運搬させることが可能になった。
【0014】
【配列表】
[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for effectively introducing active compounds into termite nests.
[0002]
[Prior art and problems to be solved by the invention]
There is a method for killing termites by injecting a solution-type drug into an intrusion site. However, this method has only a temporary effect and required a large amount of chemicals to penetrate the wood sufficiently. Furthermore, if only the damaged part is removed, the termites will change the passage and move the damage to other parts, which makes the removal more difficult. Frequently practiced is fumigation using methyl bromide. However, methyl bromide is a causative substance of the ozone layer depletion, and in recent years there has been an increasing trend to restrict its use. There is a method of exterminating ants that live in the same way as termites, such as mixing the ant's favorite food with poisons as food, and bringing it back to the nest to kill the entire population. However, since termites feed on the nesting wood itself, it is not possible to apply a method of transporting drugs from the outside of the nest to the inside of the nest using poisonous bait.
[0003]
[Means for Solving the Problems]
As a result of studying a method for efficiently introducing an active compound into a termite nest, the present inventor focused on termite egg recognition and egg transport behavior, and used an egg recognition substance extracted from an egg as a base material imitating an egg. I found a method to efficiently transport it into the nest by applying it, and found this book. Termite ants have the property of taking care of the eggs born by transporting them in piles and licking the surface of the eggs. A craft ant has a size and shape similar to a termite egg, recognizes a pseudo egg containing an egg recognizing substance on the surface as an egg, and carries it into its own egg mass in the nest. It is possible to efficiently destroy the reproductive center of the colony by containing an active compound such as an insecticidal active compound or a hatching inhibitor in the simulated egg and transporting it into the egg mass. The present inventor has also discovered a new species of Aceria sp. Nov., Which forms a sclerotia mimicking the eggs of Yamato termites and co-exists in the egg mass. The filamentous fungus can grow and nucleate on a PDA (potato-dextrose agar) medium, and the sclerotium obtained by culturing contains an active compound and an egg recognizing substance and can be transported to termites. Is possible.
[0004]
DETAILED DESCRIPTION OF THE INVENTION
In order for a termite to recognize an object other than a termite egg as an egg and transport it into the nest, it is necessary to physically and chemically resemble the object. In the present invention, a molded body having a spherical shape having a diameter of 0.3 to 0.45 mm or a mycelium is used as a base material, and the surface containing a termite egg recognition substance is used as a pseudo egg. An actual termite egg has a long egg shape, and a long egg shaped pseudo egg may be used, but a spherical shape is easier to form.
[0005]
As a method for incorporating a termite egg recognizing substance into a base material, an egg mass is collected from a termite nest, an egg surface material is extracted using ether as a solvent, and this is impregnated and applied to the base material. The egg recognition substance extracted from the termite egg has the same effect on the termite. This pseudo egg is injected into a termite ant canal or into an intrusion site and transported to a craft ant. Once the transport is started, the artificial ants are transported one after another so that the mimetic egg can be efficiently introduced into the center of the nest.
[0006]
There are various materials suitable as the base material for producing the simulated egg used in the present invention. For example, in addition to thermoplastic resins such as polyethylene, polypropylene, polystyrene, polyester, polyvinyl chloride, and polycarbonate, or thermosetting resins such as urea resin, epoxy resin, phenol resin, and polyurethane, porous gelation such as silica gel Agents, glass and the like. A material obtained by molding these materials into a spherical shape having a diameter of 0.3 to 0.45 mm can be used as a base material for a pseudo egg. Alternatively, sclerotia obtained by artificial culture of termite symbiotic sclerotia can be used as a substrate. Suitable materials are not limited to these examples, but materials whose material specific gravity is significantly different from eggs are not transported.
[0007]
The mimetic egg used in the present invention can contain an active compound as long as the transport rate is not impaired. Examples include pyrethroid compounds, organophosphorus compounds, carbamate compounds, N-aryldiazole compounds, hydrazone compounds, sulfonamide compounds, natural insecticidal component compounds, as well as hatching inhibitory substances, juvenile hormone-like substances, etc. And other insect growth control substances. Examples of the method of incorporating the active compound in the pseudo egg include a method of kneading into a resin or the like, or impregnating and applying to a porous body.
[0008]
【Example】
Next, although the manufacture example and test example of the pseudo egg of this invention are shown, this invention is not limited only to the following examples.
[0009]
Production Example 1 (pseudo egg based on mycorrhiza): 94.0% by weight of jetyl ether was added to 6.0% by weight of a termite egg, and ultrasonically washed for 1 minute. This was filtered to remove the egg to obtain an egg surface substance extraction solution (1). After adding 96.5% by weight of the egg extract solution (1) to 3.5% by weight of the termite symbiotic sclerotia obtained by culturing in the PDA medium, the ether solvent is volatilized to remove the egg on the surface of the sclerotia. A recognition substance is attached.
[0010]
Production Example 2 (Pseudo Egg Based on Glass Beads): Egg Extract Solution (1) described in Production Example 1 to 12.5% by weight of glass beads having a diameter of 0.4 mm (manufactured by Reciprocal Chemical Glass Co., Ltd.) After adding 87.5% by weight, the ether solvent is volatilized to attach the egg recognizing substance to the surface of the glass beads.
[0011]
Comparative example (when sea sand is used as a base material): 30 mesh sea sand (manufactured by Wako Pure Chemical Industries, Ltd.) 7.0% by weight, 93.0% by weight of egg extraction solution (1) described in Production Example 1 After the addition, the ether solvent is volatilized to attach the egg recognition substance to the surface of the sea sand.
[0012]
Test example: 20 termite eggs and 20 simulated eggs obtained in Production Example 1 were randomly sprinkled on a petri dish with a diameter of 9 cm, and 30 termite ants were placed in this, and allowed to stand in a constant temperature room at 25 ° C. for 15 hours. I put it. Professional ants gathered eggs scattered on the petri dish to form egg masses. When the egg mass was examined after 15 hours, 90% or more of the mimetic eggs were transported into the egg mass. When the carrying rate of the simulated eggs obtained in Production Example 2 and Comparative Example was examined by the same test method, the simulated eggs of Production Example 2 were transported by 40% or more, but the simulated eggs of Comparative Example were not transported at all. . Further, when 50 pseudo eggs obtained in Production Example 1 were injected into the ant canal formed by termite colonies bred indoors, after 24 hours, over 80% of the pseudo eggs were carried into the egg mass in the nest.
[0013]
【The invention's effect】
According to the present invention, an artificial object can be efficiently transported into a pest nest for the purpose of pest control.
[0014]
[Sequence Listing]
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19487099A JP4151812B2 (en) | 1999-06-04 | 1999-06-04 | Pest control method by fake egg transportation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP19487099A JP4151812B2 (en) | 1999-06-04 | 1999-06-04 | Pest control method by fake egg transportation |
Publications (2)
Publication Number | Publication Date |
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JP2000342149A JP2000342149A (en) | 2000-12-12 |
JP4151812B2 true JP4151812B2 (en) | 2008-09-17 |
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Application Number | Title | Priority Date | Filing Date |
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JP19487099A Expired - Lifetime JP4151812B2 (en) | 1999-06-04 | 1999-06-04 | Pest control method by fake egg transportation |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011059054A1 (en) | 2009-11-13 | 2011-05-19 | 国立大学法人 岡山大学 | Termite egg disinfestation technique utilizing volatile calling pheromone and queen pheromone |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4126379B1 (en) * | 2007-02-15 | 2008-07-30 | 国立大学法人 岡山大学 | Pest control using lysozyme, its salts, biological fragments or related peptides as egg recognition pheromones |
JP5508685B2 (en) * | 2008-03-18 | 2014-06-04 | 国立大学法人 岡山大学 | Extinguishing technology using ant's brood protection behavior |
JP4258785B1 (en) | 2008-06-02 | 2009-04-30 | 国立大学法人 岡山大学 | Simulated eggs using β-glucosidase as a constituent of termite egg recognition pheromone and termite control using it |
WO2013073676A1 (en) | 2011-11-16 | 2013-05-23 | 森下仁丹株式会社 | Pest control agent |
ES2749213T3 (en) | 2013-10-04 | 2020-03-19 | Univ Kyoto | Seamless capsule for termite extermination |
JP6166638B2 (en) * | 2013-10-16 | 2017-07-19 | 住化エンバイロメンタルサイエンス株式会社 | Termite control agent |
JP6189207B2 (en) * | 2013-12-25 | 2017-08-30 | 住化エンバイロメンタルサイエンス株式会社 | Termite control agent |
-
1999
- 1999-06-04 JP JP19487099A patent/JP4151812B2/en not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011059054A1 (en) | 2009-11-13 | 2011-05-19 | 国立大学法人 岡山大学 | Termite egg disinfestation technique utilizing volatile calling pheromone and queen pheromone |
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