JP3961429B2

JP3961429B2 - Nutritional enhancement composition for feed or feed

Info

Publication number: JP3961429B2
Application number: JP2003012683A
Authority: JP
Inventors: 哲夫山根; 治男福原; 仁志長崎
Original assignee: 哲夫山根; 宗村秀夫
Priority date: 1998-04-24
Filing date: 2003-01-21
Publication date: 2007-08-22
Anticipated expiration: 2019-04-23
Also published as: JP2003199510A

Description

【０００１】
【発明の属する技術分野】
本発明は飼料又は餌料用栄養強化組成物及びこの飼料又は餌料用栄養強化組成物を添加した飼料又は餌料に関するものである。
一般に、家畜の飼育や魚介の養殖には大量の飼料や餌料が使用されている。
本発明の飼料又は餌料用栄養強化組成物を添加した飼料又は餌料は家畜や養殖魚介がよく食すようになり、これらの体重増加を促進するので、家畜の飼育界や魚介の養殖界に大きく貢献するものである。
【０００２】
【従来の技術】
従来、ニワトリの飼育やヒラメの養殖などは広く行なわれており、これに使用する飼料や餌料についても体重増加を促進するための各種添加物が研究され、種々試みられているが、決定的にすぐれた添加物は未だ見出されていないのが現状である。
【０００３】
【発明が解決しようとする課題】
本発明では、従来ニワトリやタイなどが比較的よく食す魚介類の処理物について、より濃縮された状態において添加物として、すぐれた飼料や餌料を提供することを課題とした。
【０００４】
【課題を解決するための手段】
本発明において、魚介類から選んだ１もしくは２以上の本体、その処理物もしくはこれらからの分別物を蛋白分解酵素で酵素処理し、その酵素処理物から遠心分離処理によって分離した沈殿区分に養殖魚の成長を促進する成分が多量含まれることが見出されたのである。
従来、魚体に蛋白分解酵素処理することは知られている（特開昭６４−３０５６０号公報）が、この公報に記載されていることは、魚体を蛋白分解酵素処理して得たスラリーを三相デカンターに供給し、ケーキ、重液、軽液に分離し、この重液を濃縮してケーキと混合し、乾燥して消化の良い魚粉を製造するに過ぎないものであった。
【０００５】
本発明では、魚介類から選んだ１もしくは２以上の本体、その処理物もしくはこれらからの分別物を蛋白分解酵素による酵素処理を行い、得られた酵素処理物を遠心分離して沈殿区分を得ることを特色としている。この方法では蛋白分解酵素により、従来は蛋白質と結合して分離、濃縮が困難だった複合脂質、高度不飽和脂肪酸やステリン類を容易に分離させ、複合脂質類を高濃度に沈殿区分に残留させることを特徴とするものである。これらの複合脂質類は魚類に特有のＤＨＡやＥＰＡを構成脂肪酸とするフォスファチヂルコリンを主成分とし、ニワトリや養殖魚が好んで食し、ニワトリや養殖魚の成長や産卵を促進するのである。
【０００６】
本発明は、魚介類から選んだ１もしくは２以上の本体、その処理物もしくはこれらからの分別物を蛋白分解酵素による酵素処理を行い、酵素処理物を遠心分離処理して沈殿区分を分離し、得られた沈殿区分を、そのまま又は吸着体に吸着させ、乾燥してなる飼料又は餌料用栄養強化組成物に関するものである。
本発明の飼料又は餌料用栄養強化組成物を飼料又は餌料に少量添加した場合、ニワトリや養殖魚介の飼料又は餌料の摂取量が増加し、骨の生長を促進し、体重の増加が促進されるものである。
魚介類から選んだ１もしくは２以上の本体、その処理物もしくはこれらからの分別物を蛋白分解酵素による酵素処理を行い、酵素処理物を遠心分離処理して得られた沈殿区分が飼料又は餌料用栄養強化組成物たり得ることを見出したのは本発明がはじめてで、従来、全く知られていないことなのである。
【０００７】
本発明で用いる材料は、魚介類の１もしくは２以上で、その本体とは魚介の本体そのもので、処理物は、切断、磨砕などしたもので、また、分別物は、取り出した内臓など廃棄するようなものも含むものであり、例えばオキアミ、イカの皮、魚介のハラワタ、シジミ、大魚の頭、尾やヒレなどから選んだものやこれらの混合物で、その本体全部でもよくまた切断したものや、イカの皮や内臓、エビの内臓など廃棄するような分別物などが好ましい。
これら材料は、ミキサーにかけて、全体を微細なものとし、酵素反応を容易なものとする。
【０００８】
これに蛋白分解酵素が０．０１〜３重量％、好ましくは０．０２〜２重量％添加され、３０〜６０℃で、１〜２時間程度攪拌しつつ反応させる。
蛋白分解酵素としては、エンド型ペプチダーゼ、エキソ型ペプチダーゼ、エンド・エキソ両方型ペプチダーゼなどいすれでもよく、混合物でもよく、市販の酵素が有効に使用される。
【０００９】
酵素反応が終了したら、８０〜１００℃に加熱して酵素を失活させ、しばらく放置することによって生じた沈殿物を取得し、これを遠心分離して、沈殿区分を得る。この沈殿区分は、そのまま飼料又は餌料用栄養強化組成物となる。これを温風で水分１０％以下になるまで乾燥した後、得られた乾燥沈殿区分(栄養強化組成物)は飼料又は餌料に０．１〜１０％(沈殿区分の直接乾燥品換算で)混合してすぐれた飼料又は餌料を作成することができる。あるいは飼料に０．１〜１５％(沈殿区分の直接乾燥品換算で)混合してすぐれた抗コクシジュウム飼料とすることができる。温風による乾燥の条件は、乾燥機内の温度は８５℃前後で、約１２時間である。
【００１０】
また、遠心分離して得た沈殿区分はフスマ、ヌカなどの吸着体に吸着させて、あと乾燥させれば、粉末状の飼料又は餌料用栄養強化組成物となる。その乾燥は、上記と同様に行なわれる。得られた乾燥物は、これを飼料又は餌料に０．１〜１０％(沈殿区分の直接乾燥品換算で)混合して飼料又は餌料とすることができる。あるいは、飼料に０．１〜１５％(沈殿区分の直接乾燥品換算で)混合して鶏用のすぐれた抗コクシジュウム飼料とすることができる。温風による乾燥の条件は、乾燥機内の温度は８５℃前後で、約１２時間である。
【００１１】
また、魚介類から選んだ１もしくは２以上の本体、その処理物もしくはこれらからの分別物を蛋白分解酵素による酵素処理を行い、この酵素処理物もしくは酵素処理物から分離した沈殿区分に溶剤を添加し、溶剤抽出を行い、溶剤抽出物を得、これより溶剤を分離して油状物を得、必要に応じてこの油状物を吸着体に吸着させて固状物とし、得られた油状物又はその粉末固状物からなる飼料又は餌料用栄養強化組成物とすることもできる。
【００１２】
即ち、先に得られた蛋白分解酵素処理物又は酵素処理物から分離した沈殿区分に溶剤を添加し、溶剤抽出処理を行い、抽出物から溶剤を分離して、脂質混合物を油状物として分離することができる。
溶剤としては脂質がよく溶解するものであればいかなるものでもよいが、クロロホルム：メタノール＝１：１の混合溶剤が好ましい。
【００１３】
溶剤抽出処理し、抽出物から溶剤を分離して得た油状物は、フスマ、ヌカなどの吸着体に吸着させれば、そのまま粉末固状物となる。
ここに得られる油状物又は粉末固状物は飼料又は餌料に０．１〜１０％(沈殿区分の直接乾燥品換算であり、以下の参考例においても同じ)混合してすぐれた飼料又は餌料を作成することができる。
【００１４】
【発明の効果】
本発明の飼料又は餌料用栄養強化組成物は、蛋白分解酵素による処理によって魚介類に存在するきわめて多くの種類の脂質成分を分解、分離することなく、そのままそっくり組成物としたので、これを含む飼料又は餌料は動物や魚介の食いつきがよく、骨の成長を促進し、成長をうながし、体重増加を促進することができる。
以下に実施例及び参考例を示すが、そこに示す％は重量％を表す。
【００１５】
【実施例１】
生イカの皮１Ｋｇに水１Ｋｇを加え、ミキサーで磨砕し、全体を５００ｒｐｍで攪拌しながら。５０℃とし、これにエンド型のプロテアーゼＹＰ−ＳＳ（ヤクルト薬品製）０．５％及びエキソ型アロアーゼＰ−１０（ヤクルト薬品製）０．５％を水に溶かして添加し、１時間酵素反応させ、９０℃で１５分処理し、酵素失活させ、反応物を遠心分離処理して、沈殿区分を得、これをそのままあるいは乾燥し、飼料又は餌料用栄養強化組成物とした。
【００１６】
【実施例２】
凍結オキアミ１Ｋｇに水１Ｋｇを加え、ミキサーで磨砕し、全体を、５００ｒｐｍで攪拌しながら、５０℃とし、これにエンド型のプロテアーゼＹＰ−ＳＳ（ヤクルト薬品製）０．５％及びエキソ型アロアーゼＰ−１０（ヤクルト薬品製）０．５％を水に溶かして添加し、１時間酵素反応させ、９０℃で１５分処理し、酵素失活させ、反応物を遠心分離処理して、沈殿区分を得、これをそのまま１Ｋｇのフスマの微細粉砕物と混合し、風乾し、これを飼料又は餌料用栄養強化組成物とした。
【００１７】
【参考例１】
凍結イワシ内臓１Ｋｇに水１Ｋｇを加え、ミキサーで磨砕し、全体を、５００ｒｐｍで攪拌しながら、５０℃とし、これにエンド型のプロテアーゼＹＰ−ＳＳ（ヤクルト薬品製）０．５％及びエキソ型アロアーゼＰ−１０（ヤクルト薬品製）０．５％を水に溶かして添加し、１時間酵素反応させ、９０℃で１５分処理し、酵素失活させ、反応物を遠心分離処理して、沈殿区分を得、これにクロロホルム：メタノール＝１：１の混合溶剤を２Ｋｇ加え、よく攪拌し、溶剤部分をとり、これから溶剤を分離し、油状物を得、これをそのまま飼料又は餌料用栄養強化組成物とした。
【００１８】
【実施例３】
家禽（ブロイラー試験−１）
ふ化から３週間の試験、２４０羽の初生ヒナ(雌雄同羽数)を２区に分け、次ぎの飼料を給与した。
飼料と試験区トウモロコシ６０％、大豆粕２２％、ふすま７％、コーングルテンミール８％、にビタミン、ミネラル等を含む全植物性飼料を対照区とし、対照区のコーングルテンミール０．５％を実施例１の乾燥した栄養強化組成物０．５％で代替した飼料を試験区とした。

【００１９】
【実施例４】
家禽（産卵鶏雄ヒナ試験−２）
ふ化から６週間の試験、４００羽の初生ヒナを４区に分け、次ぎの飼料を給与した。
飼料と試験区トウモロコシ６０％、大豆粕２２％、ふすま７％、コーングルテンミール５％、にビタミン、ミネラル等を含む全植物性飼料を対照１区とし、対照１区のコーングルテンミールの３％を魚粉に代替したものを対照２区とした。また対照１区のコーングルテンミール０．４５％を実施例２の栄養強化組成物０．４５％に代替したものを試験１区とし、０．９％代替したものを試験２区とした。

考察：全植物性飼料に対し、魚粉を３％代替添加した対照２区は約４％成長が促進され、骨の成長が７％促進された。対照１区に本栄養強化組成物を０．４５〜０．９％添加すると対照２区と同じ成長と骨の成長が認められた。従来から魚粉は雛に対する成長促進効果（ＵＧＦ）があるとされてきたが、本栄養強化組成物は魚粉のＵＧＦと同じものを含有すると考えられる。また本栄養強化組成物の添加で骨の成長が促進されたことはこれまで報告されたことの無い全く新しい知見である。
【００２０】
【実施例５】
タイ（養殖試験−３）
体重６０ｇの稚魚、８週間の試験、１６０尾の稚魚を４区に分け、次ぎの飼料を給与した。
飼料と試験区魚粉６０％、コーンフラワー１３％、魚油４％に粘結剤として小麦粉２１％にビタミン、ミネラル等を含む飼料を対照１区とし、対照１区の組成を基本として、魚粉を４０％に減らし大豆粕を２０％添加し、蛋白質含量を等しくするためにコーンフラワーをコーングルテンミールに代替したものを対照２区とした。対照１区の魚粉１％を実施例２の栄養強化組成物１％で代替したものを試験１区とし、対照２区の魚粉１％を実施例２の栄養強化組成物１％で代替したものを試験２区とした。

考察：栄養強化組成物を飼料に添加することで、稚魚の成長と骨の成長が促進された。また肥満度と増肉係数が対照区と同じか或いは低かったことは、本物質が肥満より骨の成長が望ましい稚魚の時期に好ましい効果を有することが明らかになった。
海産養殖魚の飼料は通常６０％以上の魚粉を含み、魚粉を低価格の大豆粕に代替する限度は２０％程度と考えられている。本試験では魚粉６０％を含む対照１区に栄養強化組成物を添加したところ、９％の成長促進効果を認め、尾又長と飼料効率も改善された。また魚粉４０％を含む対照２区に栄養強化組成物を添加した場合にもほとんど同じ効果が認められた。つまり本物質により大豆粕を魚粉に代替する魚粉代替効果があることが明らかになった。
【００２１】
【参考例２】
産卵鶏試験−４
３６週齢の産卵鶏各々１００羽を５６週齢まで２０週間試験した。トーモロコシ５８％、大豆粕２５％、ふすま１０％、炭酸カルシュウム５％、燐酸カルシュウム０．７％にビタミン、ミネラル等を含む飼料を対照区とし、これに参考例１の栄養強化組成物を０．２％及び０．５％添加した。

【００２２】
【参考例３】
４週齢の子豚各２０頭を１０週齢まで試験に供した。
飼料は粗蛋白質１８％−ＴＤＮ８０の市販飼料を対照区とし、これに参考例１の栄養強化組成物０．５％添加した。

栄養強化組成物を０．５％添加することで子豚の発育が促進され、生存率も改善された。
【００２３】
【参考例４】
鶏コクシジュウム病に対する効果
鶏コクシジュウム病は胞子虫類であるコクシジュウムオーチストを鶏が経口的に摂取することで感染する。コクシジュウムは慢性型と急性型に分けられるが、急性型コクシジュウムオーチストであるアイメリア・テネラやアイメリア・ネカトトリックスに感染した鶏は感染後６日ごろに出血性腸炎を起こし斃死する。斃死しない場合でも経済的に大きな損失をもたらす。
ブロイラーの初生ヒナ雄６０羽をコクシジュウム予防剤を含まない飼料で１４日間飼育した。１４日目に５羽づつ６区に分け、次の飼料を給与した。１５日目に１羽あたり160,000個の成熟したアイメリア・テネラ野外株を経口的に投与した。２１日目にヒナの体重を測定し、その後屠殺して盲腸の病変値を測定した。病変値は変化無し（０）から最大（４）までの４段階とした。

結果：コクシジュウムオーチストの投与により、対照区（1-2）と大豆油区（2-2）の盲腸は出血びん爛し、病変値は有意に増加したが、栄養強化組成物を添加した3-2区は出血はほとんど無く、病変値も０に近かった。以上から栄養強化組成物は鶏コクシジュウム病を防止するか、又は軽減する効果があることが明らかになった。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a nutritional enhancement composition for feed or feed and a feed or feed to which the nutritional enhancement composition for feed or feed is added.
In general, a large amount of feed and feed are used for raising livestock and aquaculture.
The feed or feed supplemented with the feed or feed fortification composition of the present invention is eaten well by livestock and cultured seafood, and promotes weight gain of these, so it greatly contributes to the livestock breeding world and the seafood culture world. To do.
[0002]
[Prior art]
Conventionally, chicken breeding and flounder farming have been widely performed, and various additives for promoting weight gain have been studied and various attempts have been made for feed and feed used therefor. At present, excellent additives have not yet been found.
[0003]
[Problems to be solved by the invention]
An object of the present invention is to provide excellent feed and feed as an additive in a more concentrated state with respect to processed fish and shellfish that have been eaten relatively well by conventional chickens and chickens.
[0004]
[Means for Solving the Problems]
In the present invention, one or two or more main bodies selected from seafood, processed products thereof, or fractions thereof are enzymatically treated with a proteolytic enzyme, and the cultured fish is separated into precipitate sections separated from the enzyme-treated products by centrifugation. It has been found that a large amount of components that promote growth are contained.
Conventionally, it is known that a fish body is treated with a proteolytic enzyme (Japanese Patent Laid-Open No. 64-30560). However, this publication describes that a slurry obtained by treating a fish body with a proteolytic enzyme is classified into three. It was supplied to a phase decanter and separated into cake, heavy liquid, and light liquid. The heavy liquid was concentrated and mixed with the cake, and dried to produce digestible fish meal.
[0005]
In the present invention, one or two or more main bodies selected from seafood, processed products thereof, or fractions thereof are subjected to enzyme treatment with a proteolytic enzyme, and the obtained enzyme-treated product is centrifuged to obtain a precipitation section. It is featured. In this method, proteolytic enzymes are used to easily separate complex lipids, polyunsaturated fatty acids, and sterins that were conventionally difficult to separate and concentrate by binding to proteins, leaving complex lipids in the precipitate section at high concentrations. It is characterized by this. These complex lipids are mainly composed of phosphatidylcholine having DHA and EPA, which are peculiar to fish, as constituent fatty acids, and are favored by chickens and farmed fish to promote growth and egg laying of chickens and farmed fish.
[0006]
In the present invention, one or two or more main bodies selected from seafood, processed products thereof, or fractions thereof are subjected to an enzyme treatment with a proteolytic enzyme, and the precipitates are separated by centrifuging the enzyme-treated products, The present invention relates to a fortified nutritional composition for feed or feed that is obtained as it is or adsorbed on an adsorbent and dried.
When a small amount of the feed or feed fortification composition of the present invention is added to the feed or feed, the intake of feed or feed for chickens or cultured seafood increases, promotes bone growth, and promotes weight gain. Is.
One or more main bodies selected from seafood, processed products thereof, or fractions thereof are subjected to enzyme treatment with proteolytic enzymes, and the precipitates obtained by centrifuging the enzyme-treated products are for feed or feed It is the first time that the present invention has been found that it can be a fortified composition, and it has never been known before.
[0007]
The material used in the present invention is one or more of seafood, the main body is the main body of the seafood itself, the processed product is cut and ground, and the separated product is discarded, such as the internal organs taken out. For example, krill, squid skin, sea bream, rainbow trout, large fish head, tail, fins, etc. In addition, a fraction such as squid skin and internal organs and shrimp internal organs that are discarded are preferable.
These materials are passed through a mixer to make the whole fine and facilitate the enzyme reaction.
[0008]
Proteolytic enzyme is added to this by 0.01 to 3% by weight, preferably 0.02 to 2% by weight, and reacted at 30 to 60 ° C. with stirring for about 1 to 2 hours.
As the proteolytic enzyme, any of an endo-type peptidase, an exo-type peptidase, an endo-exo-peptidase, a mixture thereof, and a commercially available enzyme can be used effectively.
[0009]
When the enzyme reaction is completed, the enzyme is deactivated by heating to 80 to 100 ° C., and a precipitate generated by leaving it for a while is obtained, and this is centrifuged to obtain a precipitation section. This precipitation classification becomes the nutrition fortification composition for feed or feed as it is. After drying this with warm air until the water content becomes 10% or less, the obtained dry precipitation category (nutrient enriched composition) is mixed with feed or feed in an amount of 0.1 to 10% (in terms of direct dry product in the precipitation category). A good feed or feed can then be made. Alternatively, it can be mixed with 0.1 to 15% of the feed (in terms of a direct dry product in the precipitation section) to obtain an excellent anti-coccidium feed. The drying conditions with warm air are about 85 ° C. and about 12 hours in the dryer.
[0010]
Moreover, if the sediment classification obtained by centrifugation is adsorbed on an adsorbent such as bran and nutka and then dried, it becomes a powdery feed or feed fortified composition. The drying is performed as described above. The obtained dried product can be mixed with a feed or a feed in an amount of 0.1 to 10% (in terms of a direct dry product in a precipitation section) to obtain a feed or a feed. Alternatively, it can be mixed with the feed in an amount of 0.1 to 15% (in terms of a direct dry product in the precipitation section) to provide an excellent anti-coccidium feed for chickens. The drying conditions with warm air are about 85 ° C. and about 12 hours in the dryer.
[0011]
In addition, one or two or more main bodies selected from seafood, processed products thereof, or fractions thereof are subjected to enzymatic treatment with proteolytic enzymes, and a solvent is added to the enzyme-treated products or precipitation sections separated from the enzyme-treated products. Solvent extraction is performed to obtain a solvent extract, from which the solvent is separated to obtain an oily substance. If necessary, this oily substance is adsorbed on an adsorbent to form a solid substance, and the obtained oily substance or It can also be set as the feed or feed fortification enhancement composition which consists of the powder solid.
[0012]
That is, a solvent is added to the previously obtained proteolytic enzyme treatment product or the precipitate section separated from the enzyme treatment product, solvent extraction treatment is performed, the solvent is separated from the extract, and the lipid mixture is separated as an oily substance. be able to.
Any solvent can be used as long as it dissolves lipids well, but a mixed solvent of chloroform: methanol = 1: 1 is preferable.
[0013]
The oily substance obtained by the solvent extraction treatment and separating the solvent from the extract becomes a powdery solid as it is when adsorbed on an adsorbent such as bran and nuka.
The oily or powdery solids obtained here are mixed with 0.1% to 10% of feed or feed (in terms of direct dry product in the precipitation category, and the same in the following reference examples). Can be created.
[0014]
【The invention's effect】
The nutrition enhancing composition for feed or feed according to the present invention includes a composition as it is as it is without decomposing and separating a very large number of lipid components present in fish and shellfish by treatment with proteolytic enzymes. The feed or feed is good for animals and seafood, can promote bone growth, promote growth, and promote weight gain.
Examples and Reference Examples are shown below, and the percentages shown here represent% by weight.
[0015]
[Example 1]
Add 1 kg of water to 1 kg of raw squid skin, grind with a mixer, and stir the whole at 500 rpm. At 50 ° C., 0.5% of endo-type protease YP-SS (manufactured by Yakult Yakuhin) and 0.5% of exo-type alloase P-10 (manufactured by Yakult Yakuhin) were dissolved in water and added to the enzyme reaction for 1 hour. The mixture was treated at 90 ° C. for 15 minutes to inactivate the enzyme, and the reaction product was centrifuged to obtain a precipitate section, which was left as it was or dried to obtain a nutrient enriched composition for feed or feed.
[0016]
[Example 2]
1 Kg of water is added to 1 Kg of frozen krill, ground with a mixer, and the whole is stirred at 500 rpm to 50 ° C., with 0.5% endo-type protease YP-SS (manufactured by Yakult Yakuhin) and exo-type aroase P-10 (manufactured by Yakult Yakuhin) 0.5% dissolved in water, added, allowed to react with enzyme for 1 hour, treated at 90 ° C for 15 minutes, deactivated, centrifuged the reaction product, and separated into precipitates This was directly mixed with 1 kg of finely pulverized bran and air-dried to obtain a nutritional enhancement composition for feed or feed.
[0017]
[Reference Example 1]
1 kg of water was added to 1 kg of frozen sardine viscera, ground with a mixer, and the whole was stirred at 500 rpm to 50 ° C., and endo type protease YP-SS (manufactured by Yakult Yakuhin) 0.5% and exo type Aloase P-10 (manufactured by Yakult Yakuhin Co., Ltd.) 0.5% dissolved in water and added, allowed to react with the enzyme for 1 hour, treated at 90 ° C. for 15 minutes, deactivated, centrifuged the reaction product, and precipitated. Add 2kg of mixed solvent of chloroform: methanol = 1: 1 to this, stir well, take the solvent part, separate the solvent from this, obtain oil, and use it as it is for nutritional enhancement composition for feed or feed It was a thing.
[0018]
[Example 3]
Poultry (broiler test-1)
In the test for 3 weeks after hatching, 240 newborn chicks (number of males and females) were divided into 2 wards and fed the next feed.
Feed and test plots Corn 60%, soybean meal 22%, bran 7%, corn gluten meal 8%, whole plant feed containing vitamins, minerals, etc. as the control plot, and control corn gluten meal 0.5% The feed replaced with 0.5% of the dry fortified composition of Example 1 was used as a test plot.

[0019]
[Example 4]
Poultry (spawning chicken chick test-2)
Six weeks after hatching, 400 newborn chicks were divided into 4 wards and fed the next feed.
Feed and test plots 60% corn, 22% soybean meal, 7% bran, 5% corn gluten meal, 5% corn gluten meal, and the whole plant feed containing vitamins, minerals, etc. The control was replaced with fish meal. Moreover, what substituted 0.45% of corn gluten meal of the control 1 group for the nutrient enhancement composition 0.45% of Example 2 was made into test 1 group, and what substituted 0.9% was made into test 2 group.

Discussion: Compared to the whole plant diet, the control 2 group supplemented with 3% fish meal promoted about 4% growth and promoted bone growth by 7%. When 0.45 to 0.9% of this nutritional enhancement composition was added to the control 1 group, the same growth and bone growth as in the control 2 group were observed. Conventionally, fish meal has been considered to have a growth promoting effect (UGF) on chicks, but it is considered that the present nutrition-enriched composition contains the same UGF as fish meal. In addition, the fact that the growth of bone was promoted by the addition of the present nutrition-enriched composition is a completely new finding that has never been reported.
[0020]
[Example 5]
Thailand (Aquaculture Test-3)
The fry weighing 60 g, the 8-week test, and 160 fry were divided into 4 wards and fed the next feed.
Feed and test plots Fish meal 60%, corn flour 13%, fish oil 4% as a binder and wheat flour 21% feed containing vitamins, minerals, etc. The control 2 was obtained by adding 20% soybean meal and reducing corn flour to corn gluten meal in order to equalize the protein content. 1% of the fish meal in the control 1 ward was replaced with 1% of the fortified composition of Example 2 as test 1 ward, and 1% of fish meal in the 2nd ward of control was replaced with 1% of the fortified composition of Example 2 Was designated as test 2 section.

Discussion: The addition of a fortified composition to the feed promoted fry growth and bone growth. In addition, the fact that the obesity level and the coefficient of increase in meat were the same as or lower than those in the control group revealed that the substance has a favorable effect at the time of fry where bone growth is more desirable than obesity.
Marine aquaculture fish feed usually contains more than 60% fish meal, and the limit of replacing fish meal with low-cost soybean meal is considered to be around 20%. In this test, when the nutrient fortifying composition was added to the control 1 group containing 60% fish meal, a growth promoting effect of 9% was observed, and the tail length and feed efficiency were also improved. In addition, almost the same effect was observed when the fortified composition was added to the control 2 section containing 40% fish meal. In other words, it was clarified that this substance has a fish meal substitute effect that substitutes soybean meal for fish meal.
[0021]
[Reference Example 2]
Laying hen test-4
Each 36-week-old laying hen was tested for 20 weeks until 56-week-old. A diet containing vitamins, minerals, etc. in 58% corn, 25% soybean meal, 10% bran, 5% calcium carbonate, 0.7% calcium phosphate, was used as a control group. 2% and 0.5% were added.

[0022]
[Reference Example 3]
Twenty 4-week-old piglets were used for the test until the age of 10 weeks.
For the feed, a commercial feed of crude protein 18% -TDN80 was used as a control, and 0.5% of the fortified composition of Reference Example 1 was added thereto.

By adding 0.5% of the fortified composition, the growth of the piglet was promoted and the survival rate was also improved.
[0023]
[Reference Example 4]
Effect on chicken coccidiosis Chicken coccidiosis is transmitted when chickens orally ingest sporeworm coccidiosis. Coccidium can be divided into chronic and acute types, but chickens infected with acute coccidium oocysts, Eimeria tenella and Eimeria necatholics, develop hemorrhagic enteritis around 6 days after infection and die. Even if it does not drown, it causes a large economic loss.
Sixty new broiler chicks were bred for 14 days on a feed containing no coccidium preventive agent. On the 14th day, 5 chicks were divided into 6 wards, and the following feed was fed. On day 15, 160,000 mature Eimeria tenella field strains per bird were orally administered. On day 21, chicks were weighed and then sacrificed to determine cecal lesion values. The lesion values were classified into 4 levels from no change (0) to the maximum (4).

Results: The administration of coccium oocysts caused hemorrhagic vaginal discharge in the cecum in the control group (1-2) and soybean oil group (2-2), and the lesion value increased significantly, but the nutritional enhancement composition was added. In District 3-2, there was almost no bleeding and the lesion value was close to zero. From the above, it has been clarified that the fortified composition has an effect of preventing or reducing chicken coccidiosis.

Claims

Using at least one material selected from krill, rainbow trout, large fish head, tail and fin, seafood shellfish, squid skin and internal organs, and shrimp internal organs, this is subjected to enzymatic treatment with proteolytic enzymes to convert complex lipids into proteins Was separated from the enzyme-treated product by heating to inactivate the proteolytic enzyme and centrifuging it to separate the precipitate fraction that left the complex lipids in high concentration. A nutrition enhancing composition for feed or feed containing a high concentration of complex lipids, characterized in that the precipitate section is adsorbed as it is or adsorbed on an adsorbent selected from bran or Nuka and dried.

A chicken or aquaculture comprising the feed or feed fortified composition according to claim 1 mixed with the feed or feed in an amount of 0.45 to 0.9% (in terms of a direct dry product in the precipitation section). Feed or feed for fish.