JP3898887B2 - Thermoblock rotator - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention rotates a reaction vessel containing a sample and a reaction solution fixed to a filter membrane mainly for molecular biology / biochemistry experiments such as DNA / DNA hybridization, DNA / RNA hybridization, antigen-antibody reaction, etc. in a thermoblock. The present invention relates to a rotating device of a reaction tank under a constant temperature, that is, a thermoblock rotator.
[0002]
[Prior art]
Conventionally, when an experimental sample fixed to a filter membrane is reacted in a solution, a plastic bag is used as a reaction vessel and a water bath incubator is used as a thermostatic bath. However, bubbles generated during the reaction easily adhere to the filter membrane. There was a drawback that non-uniformity occurred in the reaction.
As a means for solving this problem, a method is currently used in which a filter membrane and a reaction solution are placed in a cylindrical tube and reacted in a gas phase incubator.
However, the gas phase incubator has a drawback that there is a time lag until the solution in the reaction vessel reaches a constant temperature, and that the temperature in the incubator tends to be uneven. In addition, since the capacity of the experimental apparatus is increased, there is a problem that the proportion of the experimental apparatus is increased.
Therefore, the solution in the inserted reaction tank quickly reaches a constant temperature, can stably maintain the set temperature, and can achieve uniform reaction by effective stirring, and can be used as a small reaction tank that is inexpensive in terms of cost. Improvement of an experimental device that can use a tube has been desired.
[0003]
Problems to be Solved by the Invention
The present invention eliminates the above-mentioned difficulties, has a highly accurate temperature control ability compared to conventional gas phase incubators, and enables highly reproducible reactions by ensuring uniform reaction by rotary stirring. An object was to provide a thermo block rotator using a small thermo block.
[0004]
[Means for Solving the Problems]
The present invention is a temperature capable of controlling the aluminum block in the metallic assembly housing, primarily the length of the reaction tank containing a reaction vessel the sample and reaction solution was fixed to the filter membrane for molecular biology, biochemistry experiment It is a device that is inserted so that it can rotate about a horizontal axis as a substantially horizontal axis, and performs the reaction under constant temperature conditions. The reaction tank inserted in the aluminum block is rotated, and further, the rotation is vertically vertical. A thermoblock rotator that rotates the reaction vessel in a thermoblock for molecular biology and biochemistry experiments, which stirs the reaction solution by adding the seesaw motion of, so that a uniform reaction is performed in the reaction vessel. It is a summary.
[0005]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 1 is a front view showing an outline of an apparatus according to the present invention, and FIG. 2 is a rear view showing an outline of a drive unit and a rotation mechanism of the apparatus of FIG.
FIG. 3 is a perspective view of essential parts for rotating the reaction tank inserted in the constant temperature aluminum block of the apparatus, and FIG. 4 is an exploded perspective view of the reaction tank insertion part.
Note that the insertion direction of the aluminum block reaction tank and the cross-sectional shape of the orthogonal cross section may be a quadrilateral shape or a circular shape. If this is a square shape, the Peltier element 11 is mounted on the aluminum block as shown in FIG. radiating fin 12 at the top, suitable for aluminum block upper surface in relation to the mounting cooling fan 13 in a horizontal plane, if the constant temperature is 2, aluminum block as shown in FIG. 3 or FIG. 4 may be circular .
[0006]
The aluminum block 6 serving as the core of the thermoblock rotator 2 is a square or circular block, and an insertion hole 16 of the reaction tank 15 is provided in the block in the lateral direction (axial direction). The reaction tank 15 shown in FIG. 2 is pivotally inserted and rotated.
A plurality of the insertion holes 16 are formed through one aluminum block 6 as shown in each drawing. At this time, as shown in FIG. 2, the insertion holes 16 are arranged at equal intervals around the heater 4.
In addition, as shown in FIG. 2, the reaction tank 15 inserted into each insertion hole 16 of each reaction tank 15 has an annular drive belt 7 that is rotated by the driving force of one motor m. Each reaction tank 15 is rotated in conjunction with the periphery of the end portion.
As the reaction vessel, a glass tube may be used, but a low-cost, small-capacity commercial plastic tube with high versatility is mainly used.
In addition, a heater is attached to the aluminum block, and the rotating action of the reaction vessel is operated under constant temperature conditions. In particular, DNA / DNA hybridization between the experimental sample on the filter membrane placed in the reaction vessel and the substance in the solution, DNA / Even in RNA hybridization and antigen-antibody reaction, it enables efficient experiments in small containers. The aluminum block is provided with a temperature sensor 18.
[0007]
Regarding the rotational movement of the reaction tank, since uniform reaction conditions are required in DNA / DNA hybridization, DNA / RNA hybridization, and antigen-antibody reaction, a method of rotating a cylindrical tube is effective. Has used a rotator using a gas phase incubator, but the thermo block rotator using a constant temperature aluminum block according to the present invention has a temperature difference of ± 0 in comparison with a conventional gas phase incubator. It was proved by the execution test that it has the ability to control with high accuracy of 5 ℃.
[0008]
The rotation of the rotator in the aluminum block, insert the reaction vessel to a temperature adjustable aluminum block, and may give out an homogeneous reaction acts reaction vessel by rotating the reaction vessel, relates view to claim 2 5 As shown in FIG. 6 , the reaction vessel is rotated and the entire aluminum block is vertically and vertically seesaw motioned with the rear part of the aluminum block as a fulcrum to stir to control a uniform reaction under constant temperature conditions. Other devices are also possible.
[0009]
【The invention's effect】
As described above, the present invention is a small tester with a weight of about 5 kg, a highly accurate temperature control capability, and an experimental device that performs uniform reaction by rotary stirring, enabling highly reproducible experiments in the fields of physics and chemistry, mainly molecular biology and biochemistry. It is possible to provide a thermo block rotator using a small thermo block.
[Brief description of the drawings]
FIG. 1 is a schematic front view of the apparatus.
FIG. 2 is a schematic rear view of a drive unit and a rotation mechanism of the apparatus.
FIG. 3 is a perspective view of essential parts for rotating a reaction tank inserted in a constant temperature aluminum block of the apparatus.
FIG. 4 is an exploded perspective view of a reaction tank insertion portion.
FIG. 5 is a schematic front view of a seesaw motion of an aluminum block in the vertical direction.
FIG. 6 is a schematic side view of seesaw motion of an aluminum block in the vertical direction.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Drive part 2 Thermo block rotator 4 Heater 5 Case 6 Aluminum block 7 Drive belt 9 Rotating horizontal axis 11 Peltier element 12 Discharge fin 13 Cooling fan 14 Thermal insulation cover 15 Reaction tank 16 Insertion hole 18 Temperature sensor m Motor

Claims (2)

A reaction tank containing a sample and a reaction solution mainly fixed to a filter membrane for molecular biology and biochemistry experiments in an aluminum block configured to be temperature-controllable in a metal assembly housing is the center in the length direction of the reaction tank. This is a device that inserts the wire so that it can rotate about a horizontal axis, and reacts between the experimental sample on the filter membrane and the substance in the solution under constant temperature conditions. The reaction vessel inserted in the aluminum block is rotated. A thermoblock rotator that rotates the reaction vessel in a thermoblock for molecular biology and biochemistry experiments, characterized in that the reaction solution is stirred and a uniform reaction is formed in the reaction vessel. .   The thermoblock rotator according to claim 1, wherein a seesaw motion in the vertical direction is added to the rotation of the reaction vessel inserted in the aluminum block to stir the reaction solution so that a uniform reaction is performed in the reaction vessel. A thermoblock rotator that rotates a reaction vessel in a thermoblock mainly for molecular biology and biochemistry experiments.

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