JP3723629B2 - Brain localization device and its fine movement positioning device - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a brain localization device that facilitates the adjustment work of a brain localization device in an experiment of injecting a cell marker in a brain of an animal such as a rat or mouse, and a fine movement positioning device thereof .
[0002]
[Prior art]
A conventional rat / mouse stereotaxic apparatus has a structure in which the ear bar 1 is inserted into the external auditory canal of an animal and the upper jaw is fixed to an upper jaw fixing bracket 2 to determine the position of the skull as shown in FIG. At this time, the brain is aligned by moving the height of the upper jaw fixing bracket up and down along the support column with the axis of the ear bar as a fulcrum.
At that time, in order to accurately position the head of the animal, the electrode a is first attached to the manipulator, the position of the bregma of the skull is obtained with the tip as a measurement point, the manipulator is moved, and the position of the lambda suture is also obtained.
At this time, important in determining the position of precisely the brain that the height of the lambda suture coincides with bregma, the height of the upper jaw mounting brackets stereotaxic apparatus for this, staggered along the column, the bregma Change the height. At this time, the height of the lambda stitch also changes, so measure the height again. By repeating this, the heights of the Bregma stitch and the lambda stitch are matched.
[0003]
[Problems to be solved by the invention]
In such a conventional device, the height of the maxillary fixation bracket is adjusted by eye measurement or groping, and it has been hard work to repeatedly go back and forth and adjust it to several microns or tens of microns. There is a problem.
In view of the problems of this conventional apparatus, the present invention makes it possible to accurately and quickly localize the position of the skull of an animal, and to improve the work in time, accuracy and economy. An object of the present invention is to provide a brain localization apparatus and a fine movement positioning apparatus thereof.
[0004]
[Means for Solving the Problems]
Therefore, in the brain stereotaxic device of the present invention, the upper jaw fixing bracket and ear bar that are mounted on the support provided on the fixation base so as to be movable up and down support the skull of the subject and determine the height direction position of Bregma suture and lambda suture. A stereotaxic device for fixing in position, provided with a fine movement positioning adjustment mechanism at the upper part of the support column having an adjustment knob rotating in a horizontal direction and a height adjustment screw threaded in a vertical direction; As the adjustment knob is rotated in the horizontal direction, the height adjustment screw changes the vertical position of the upper jaw fixing bracket so that the skull of the subject can be fixed at a fixed position with the support point of the ear bar as a fulcrum. It is characterized by doing so.
[0005]
Further, in the fine movement positioning device of the brain localization apparatus of the present invention, the fine movement positioning device of the brain localization apparatus includes a fixed base and an upper jaw fixing bracket that is attached to a support provided in the fixed base so as to be movable up and down. A fine positioning adjustment mechanism having an adjustment knob that rotates in a horizontal direction and a height adjustment screw that is threaded in a vertical direction is provided at an upper portion of the column, and the height of the adjustment knob as the adjustment knob rotates in the horizontal direction. The position of the upper jaw fixing bracket is changed by the height adjusting screw so that the skull of the subject can be fixed at a fixed position.
[0006]
Furthermore, in the fine movement positioning device of the brain localization apparatus of the present invention, the expansion elastic spring that is attached to the column and presses the upper jaw fixing bracket upward, and the expansion elastic spring provided above the column is applied. It has a height adjusting screw that changes the vertical position of the upper jaw fixing bracket in accordance with the horizontal rotation of the adjustment knob against the upward force.
[0007]
Further, in the fine positioning device of the brain localization apparatus of the present invention, a screw hole provided in the support column, an expansion elastic spring attached to the support column and applying upward elastic force to the upper jaw fixing bracket, and a hollow A position adjusting cylinder that covers an upper portion of the support column and a lower end side of which is attached to the upper jaw fixing metal so as to be movable up and down with respect to the support column, and from the upper end side to the lower end side of the position adjustment cylinder body It is provided so as to pass through and screw into the screw hole in the column, and with the horizontal rotation of the adjustment knob, it advances and retreats to the screw hole of the column, and the upward elastic pressure applied by the pressure-enhanced elastic spring is applied. A height adjusting screw for changing the position in the vertical direction of the upper jaw fixing bracket is provided.
[0008]
DETAILED DESCRIPTION OF THE INVENTION
FIG. 2 shows a preferred embodiment of the present invention.
In FIG. 2, reference numeral 2 is a conventionally known upper jaw fixing bracket that is supported by a support column 4 standing on a fixing base 3 so as to be movable up and down. In the illustrated embodiment, the support column 4 is passed through the through hole formed in the upper jaw fixing bracket 2.
An expanded elastic spring 5 is wound at a position below the upper jaw fixing bracket 2 of the support column 4 so as to apply an upward elastic force to the upper jaw fixing bracket.
[0009]
Above the upper jaw fixing bracket 2 of the support column 4, a position adjusting cylinder 6 made of a metal cylinder, a metal polygonal cylinder, a synthetic resin polygonal cylinder or the like is covered, and its lower end is brought into contact with the upper jaw fixing bracket 2; The upper part is positioned above the upper end of the column 4.
Then, a height adjustment screw 7 having an adjustment knob 8 at the top is inserted into the position adjustment cylinder 6 from above and screwed into the support column 4, and a collar 9 provided on the upper part of the adjustment screw 7 is provided as a position adjustment cylinder. It is in contact with the upper end of the body 6.
Further, a set screw 10 is provided at a rear portion of the upper jaw fixing bracket 2, and the upper jaw fixing bracket 2 is fixed to the support column 4 by the set screw 10.
A fine movement positioning adjustment mechanism 11 is constituted by the above-described expansion / contraction spring 5, the position adjusting cylinder 6 and the height adjusting screw 7.
[0010]
The operation of the fine movement positioning device of the present invention having the above-described configuration is as follows. First, before the operation, the set screw 10 is loosened so that the upper jaw fixing bracket 2 can be moved up and down with respect to the support column 4, and the height adjusting screw 7 is appropriately rotated by holding the adjusting knob 8 and rotating it. The height adjusting screw 7 presses the position adjusting cylinder 6 and enters the column 4, and the position adjusting cylinder 6 pushes down the upper jaw fixing bracket 2 against the expansion pressure of the elastic spring 5. On the other hand, if the adjusting knob 8 is rotated in the reverse direction, the height adjusting screw 7 advances from the support column 4, so that the pressing force against the upper jaw fixing bracket 2 of the position adjusting cylinder 6 is released, and the upper jaw fixing bracket 2 is expanded and compressed. It is pushed up by the expansion pressure of the spring 4. Therefore, the vertical position adjustment of the upper jaw fixing bracket 2 can be performed very easily.
[0011]
【The invention's effect】
The device of the present invention has the following effects.
(1) Rather than deciding the position by eye amount or groping, highly accurate adjustment can be performed very easily.
(2) Since accurate alignment is possible, the success rate of the experiment is increased, resulting in a lot of time and economic savings.
(3) The device does not require significant modification of the brain stereotaxic device.
(4) This mechanism can be added to an existing apparatus only by exchanging parts.
(5) Since nothing special in structure or mechanism is used, it is possible to provide compatibility with other models or manufacturers' stereotaxic devices.
[Brief description of the drawings]
FIG. 1 is a conceptual diagram illustrating an operation for accurately aligning a brain using a suture of a skull as a mark.
FIG. 2 is a schematic view showing an embodiment of the positioning apparatus of the present invention, in which the fine movement positioning adjustment mechanism is enlarged, partially cut away, and shown in a separate frame.
FIG. 3 is a perspective view showing the device of the present invention attached to a brain stereotaxic device.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 Ear bar 2 Upper jaw fixing bracket 3 Fixing stand 4 Support column 5 Expansion pressure spring 6 Position adjustment cylinder 7 Height adjustment screw 8 Adjustment knob 9 Buttocks 10 Set screw 11 Fine movement positioning adjustment mechanism

Claims (4)

In order to support the skull of the subject and fix the position in the height direction of Bregma stitching and lambda stitching at a fixed position by the upper jaw fixing bracket 2 and the ear bar 1 which are mounted on the column 4 provided on the fixing base 3 so as to be movable up and down. Brain localization equipment,
  A fine movement positioning adjustment mechanism 11 having an adjustment knob 8 that rotates in the horizontal direction and a height adjustment screw 7 that is threaded in the vertical direction is provided in the upper part of the column, and the horizontal adjustment of the adjustment knob is accompanied by the rotation of the adjustment knob. The position of the upper jaw fixing bracket is changed by the height adjusting screw in the vertical direction so that the skull of the subject can be fixed at a fixed position with a support point of the ear bar as a fulcrum. apparatus. A fine movement positioning device for a brain stereotaxic device comprising a fixed base 3 and an upper jaw fixing bracket 2 mounted on a support 4 provided on the fixed base so as to be vertically movable,
A fine movement positioning adjustment mechanism 11 having an adjustment knob 8 that rotates in the horizontal direction and a height adjustment screw 7 that is threaded in the vertical direction is provided in the upper part of the column, and the horizontal adjustment of the adjustment knob is accompanied by the rotation of the adjustment knob. The fine positioner of the brain stereotaxic apparatus is characterized in that the height adjustment screw changes the vertical position of the upper jaw fixing bracket so that the skull of the subject can be fixed at a fixed position. The pressure adjusting spring 5 attached to the column and pressing the upper jaw fixing bracket upward, and the adjusting force resisting the upward force provided by the expansion spring provided above the column. The fine positioning device for a brain stereotaxic device according to claim 2, further comprising a height adjusting screw (7) in which a vertical position of the upper jaw fixing bracket changes with a horizontal rotation of the eyelid. A screw hole provided in the support column, an expansion elastic spring 5 attached to the support column to apply an upward elastic force to the upper jaw fixing bracket, and is hollow and covers the upper part of the support column and the lower end side is the A position adjusting cylinder 6 that is in contact with the upper jaw fixing bracket and is mounted to be movable up and down with respect to the support column, and passes through from the upper end side to the lower end side of the position adjustment cylinder body and is screwed into a screw hole in the support column. The upper and lower fixtures are mounted in the vertical direction against the upward elastic force applied by the pressure-enhanced elastic spring as the adjusting knob is rotated horizontally in the horizontal direction of the adjusting knob. The fine movement positioning device for a brain localization device according to claim 2 or 3, further comprising a height adjusting screw (7) for changing the position.

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