JP3712007B2 - Chemical reaction visual mixing test method - Google Patents

Description

[0001]
[Industrial application fields]
The present invention relates to a chemical reaction visual mixing test method using an extremely small amount of a reagent and a sample. Implementation In addition, the results (mixing, agglomeration, coloring, sedimentation, precipitation, etc.) can be judged visually on the spot where they are mixed, and the test results can be easily carried in a pocket, pasted and stored in a record book, and saved. Therefore, it can be stored for a long time, and waste liquid treatment is unnecessary.
[0002]
[Prior art]
Conventionally, analysis, fractionation, and testing by chemical reactions such as mixing, color reaction, agglutination reaction, dissolution and precipitation (precipitation) reaction are generally performed on a test tube or a microplate, and a sample (analyte) and a chemical ( (Reagent) was put in and an analysis result was obtained with a vibration and stirring rod (Japanese Utility Model Publication No. 62-47967).
[0003]
[Problems to be solved by the invention]
However, the conventional method has recently become impossible to spill dangerous materials due to the waste liquid treatment facility or treatment method, and some incineration causes accidents that damage the incinerator due to high temperatures. Hazardous material has become a problem and infects the human body. In addition, when the amount of sample discarded is large, it can no longer be dealt with, and it has become unsuitable for the times, and ultra-trace analysis methods are desired, which are repeated in laboratories, test laboratories, laboratories, etc. Repeated tests are weighted year by year by their material costs and disposal methods.
[0004]
In addition, it can be easily put into a pocket anywhere in the room and can be used for ultra-trace testing and analysis, and the analysis results can be stored in a record as it is, without the need for a place, can be easily incinerated, and transportation costs are greatly reduced. Therefore, it is possible to eliminate the current situation of having to rely on a special analyzer for ultra-trace analysis, and to eliminate the dripping of dangerous samples and chemical samples and the facilities for that purpose at the same time.
[0005]
[Means for Solving the Problems]
Therefore, in order to eliminate the conventional problems, the present inventor is very convenient and easy to analyze in a very small amount, etc., regardless of the location, chemical reaction visual mixing test that can solve the processing problems together This method does not require a constant temperature bath or a low temperature bath as in the case of heating or low temperature processing, and a predetermined temperature can be easily set in a small cooler box using a palm-sized hot air chamber or dry ice. Various experiments and practical tests were repeated for the purpose of providing a chemical reaction visual mixing test method which can be obtained and can be performed very easily anywhere.
[0006]
As a result, it was found that if the capillary phenomenon and flexibility between the two plates were used, it was found that the same result as that in the test tube could be obtained with a very small amount, and the present invention was completed. Interest It has been confirmed that it can be used at the same time, and it has been found that this method saves considerable resources, labor costs and processing costs.
[0007]
Therefore, the chemical reaction visual mixing test method according to the present invention is provided with a mixing chamber for mixing a reagent and a sample that can be observed from the outside on a thin plate substrate having elasticity, and after the reagent and the sample are put in the mixing chamber, the substrate This is characterized in that the operation of bending is performed to mix the reagent and sample in the mixing chamber, and the result is determined by observing the mixing chamber from the outside.
[0008]
[0009]
[0010]
[Action]
The chemical reaction visual mixing test method according to the present invention is a capillary test method in which a reagent and a sample are placed in a mixing chamber (mainly a laminate of two thin plates) provided on an elastic flexible thin plate substrate so that it can be observed from the outside. Use the phenomenon or directly inject, then hold the substrate with both fingers sandwiched between fingers, then flex the substrate (restoration operation) to mix the reagent and sample in the mixing chamber, and observe the mixing chamber from the outside Then, the result (mixing, aggregation, color development, sedimentation, precipitation, etc.) is determined.
[0011]
In addition, the reagent is put into a mixing chamber of an elastic thin plate substrate provided with a mixing chamber into which a sample can enter from the outside into the chamber from outside, using a capillary phenomenon from a predetermined location, or any other arbitrary After that, the substrate is bent and restored to mix the reagent and sample in the mixing chamber, and the result is determined by observing the mixing chamber from the outside. Then, the opening is hermetically held and stored with a cover film.
[0012]
The mixing chamber is formed by superimposing a plate piece on a thin plate substrate having elasticity and fixing an arbitrary portion of the plate piece to the substrate, or forming a punched portion having a desired shape on a thin substrate having elasticity. The entire surface on the back side is closed with a cover material, and the front side is closed with an arbitrary area plate piece, or the entire surface on the front side is closed with a plate piece, and the plate piece or the cover material is provided with a through hole having a desired shape leading to the mixing chamber.
[0013]
The material of the elastic thin plate substrate is not particularly limited as long as it is elastic and can be deformed. For example, a synthetic resin thin plate is convenient, and a waterproof paper coated with a synthetic resin is used. Alternatively, a thin metal plate, a silicon coated plate, or the like can be used.
[0014]
The size, thickness, and shape of the thin plate substrate are not limited, but may be determined by the number of tests, tests for performing an analytical reaction, and the number of samples, and can be increased to 100 samples or 200 samples at a time. In a size that allows one to 300 samples to be bent (stretching) by pressing both ends of the thin plate substrate with one or both hands, naturally for a small sample, a base of about 0.05 to 0.2 mm, For 300 specimens, a base of about 0.2 to 0.5 mm, especially a business card size or telephone card size, is desirable for a small amount test (6 to 8 item inspection), and its thickness is about 0.05 to 0.5 mm. It is easy to use for many chemical reactions.
[0015]
In addition, the substrate should match the reaction color and use a color opposite to that of the reaction, and a black substrate or the like is used for white cohesive color development. Further, if a streak or a cut portion is put in the base in accordance with the plate piece, a bending effect can be obtained, and a reaction to a large amount of reaction sample is easy.
[0016]
On the other hand, the material of the plate piece does not necessarily require elasticity or flexibility, but in order to efficiently change the volume of the gap between the substrate and the plate piece, it is preferable that the flexibility is rather small or not. Since the substrate is large, it is easy to bend, but the plate piece is small and difficult to bend, so a synthetic resin thin plate is convenient. Further, the plate piece itself may be transparent so that the mixing and mixing state can be observed from the outside, or may be black or lightly colored so that aggregation, coloring (coloring), and sedimentation (precipitation) reaction can be easily seen.
[0017]
In addition, the size of this plate piece may be the same as the substrate, but usually multiple pieces smaller than the substrate are used, and each plate is stacked and fixed separately for each reaction. This is advantageous in that the test can be performed.
[0018]
In addition, the shape of the plate piece does not matter, but it is advantageous to obtain a square shape in order to easily select the fixing site and to obtain an efficient change in the gap volume accompanying the bending / restoring operation of the substrate. Even with inverted triangles and rectangles of considerable length, the mixing of the contents can be forced to stir, but rather better than mixing in a test tube. The thickness of the plate piece is about 0.05 mm to 0.5 mm as in the case of the substrate, and may be thick when there are many test specimens and thin when there are few test specimens.
[0019]
In order to fix the plate piece to the substrate, it is only necessary to fix any part. For example, when the plate piece is rectangular or rectangular, one edge, two edges, three edges, four edges, and The back cover edge portion (semicircle portion in the case of a circle) is appropriately fixed to the substrate via a temperature-deposited adhesive. In this case, a groove for the plate piece corresponding to the outer shape of the plate piece on the overlapping surface side of the substrate in advance. It is advantageous to overlap, and a groove projecting in a part of the non-fixed edge corresponding portion for the plate piece is continuously formed, and a cut, circular, semi-circular, rectangular hollow is formed. In addition to forming the sample receiving area, a groove may be formed so that the sample can easily enter the mixing chamber by capillary action. When this sample receiving area exists, excess samples and reagents accumulate in this portion and do not leak outside.
[0020]
Forming a frame for securing the necessary amount of reagents, etc. in the mixing chamber or placing reagents makes it easy to perform tests and reagent injection operations. Then, a convenient and troublesome operation on the way is unnecessary, and a quick reaction can be obtained.
[0021]
In addition, a punching part of a desired shape, for example, square, rectangle, triangle, or circle, is formed on the substrate, the back side is covered with a cover material such as black or black according to the reaction color, and a reagent is previously placed in the punching part that becomes the mixing chamber. The front side is closed with a plate piece (a plate piece slightly larger than the punched portion). At this time, in order to prevent the reagent from leaking outside, the top of the plate piece is further covered with a re-adhesive (wet) transparent or colored sample, and a cover film for preventing the reagent from being altered, and the opening is covered. Block and prevent sample evaporation and sample alteration. In this case, the position and amount of the reagent are determined by the size of the mixing chamber and the thickness of the substrate. In addition, this method makes it possible to easily and inexpensively send sample specimens and reagents to the outside and from the outside.
[0022]
When stacking the substrate and plate pieces, insert a spacer with a thickness of about 0.1 to 0.4 mm as appropriate. Ru This is desirable for more effective mixing with changes in the pore volume, especially for observing the agglomeration or centrifugation, the size and size of agglomerates during sedimentation, the amount of sediment, the time rate during sedimentation, etc. convenient.
In addition, if the substrate plate piece is rectangular and the tip is round or triangular, if the sediment deposited by centrifugation is bent with a dropper pipette, the mouth is opened and sucked, it becomes a substitute for a Spitz test tube.
[0023]
The spacer may be constituted by a member different from the substrate or the plate piece, but the plate piece may be folded and overlapped to form a spacer. In addition, it is convenient for long-term storage of reagents and the like by making a hole or a notch in the spacer and providing a frame for holding a reagent to be put in advance.
[0024]
In addition, when the spacer is made of a separate member, it is made of a water-absorbing material or made of a thread-like material. Even if the amount collected in the rectangular groove is mistakenly set, it will hardly spill out.
[0025]
Furthermore, if an appropriate number of grooves having a shape corresponding to the test is appropriately formed on the overlapping surface side of the substrate and the plate piece or the cover material, the effect of internal mixing and mixing is improved.
[0026]
Of the present invention Used in mixed test methods In order to prevent the intrusion (contamination) of foreign matter and other foreign matters (miscellaneous bacteria, etc.) both before and after use, and to prevent the storage and preservation alteration, the mixed test device is perfect for the substrate and plate pieces. It is preferable to close and seal the entire overlapping surface side with a re-adhesive cover film so that the opening of the mixing chamber can be sealed.
[0027]
Furthermore, the present invention has characteristics that each test can be carried out at a very low cost or in a very small amount for each material cost, each reagent cost, and each sample, and does not require a lot of cost for post-processing. It can be used in various fields by an epoch-making method for color analysis and mixed sedimentation test.
[0028]
【Example】
Next, an example of a mixing test device used in the mixing test method according to the present invention will be described with reference to the drawings.
[0029]
[Example 1]
First, a punched part is formed on a thin substrate, and both sides are closed with a cover material and a plate piece, To do. next, An example of a mixing test device that can be used in the mixing test method according to the present invention will be described with reference to FIGS.
[0030]
Reference numeral 1 denotes a rectangular thin plate substrate made of synthetic resin having a size of 5.5 cm × 9.0 cm and a thickness of 0.2 mm. The size and thickness can be arbitrarily selected according to the test object and the number of test samples. In addition, if it has elasticity, it can be flexibly restored (bending and stretching) regardless of its elasticity. Any material is acceptable. Further, although it may be either vertically long or horizontally long, the illustrated one is a horizontally long type. In addition, when it is carried out by a machine without holding it by hand and performing the bending restoring operation, the size and shape suitable for the machine can be obtained.
[0031]
Reference numeral 2 denotes a square punching portion formed on the thin plate substrate 1 for providing the mixing chamber 3, and a semicircular protrusion 5 is formed on the upper side portion to form the sample receiver 4. The size of the punched part is 1.2 cm × 1.2 cm. In the illustrated example, two punched portions 2 are formed, but this number can be arbitrarily selected according to the number of specimens, but by providing a plurality, it is possible to perform various tests on a single sheet, There is no need to prepare a test tool. Further, the size of the punched portion can be appropriately selected depending on the test object, the type of the reagent, and the like, and the shape may be a rectangle, a rectangle, or any other shape.
[0032]
Reference numeral 6 denotes a cover material for closing the entire back side of the punched portion 2 formed on the thin plate substrate 1. The cover material may be a material having elasticity and flexibility, or may not have these properties at all. However, it is necessary not to completely prevent the bending restoration operation of the thin substrate 1. The cover material 6 is thin so that the punched portion 2 is completely closed. Board It adheres to the substrate 1. The cover material 6 may be a transparent synthetic resin material or a metal thin plate, and any other color and material can be selected.
[0033]
Reference numeral 7 denotes a plate piece for closing the front side of the punched portion 2 formed on the thin plate substrate 1. The plate pieces are each about 1 mm larger than the punched portion 2 in the horizontal and vertical directions, and are fixed to the thin plate substrate 1 so as to close the front side of the punched portion 2. The plate piece 7 may also be made of a material having elasticity and flexibility like the cover material 6, or may have no such property at all. However, it is necessary not to completely prevent the bending restoration operation of the thin substrate 1.
[0034]
Further, the plate piece 7 may be a transparent synthetic resin material or may be a metal thin plate, and any other color and material can be selected. However, in order to make the test result observable from the outside, at least one of the plate piece 7 and the cover material 6 needs to be made of a material that allows the inside to be seen from the outside.
[0035]
The punched portion 2 of the thin plate substrate 1 is closed on the back side by the cover material 6 and the front side is closed by the plate piece 7, so that the mixing chamber 3 is formed in the punched portion 2. The protrusion formed on the upper side of the punched portion 2 is not blocked by the plate piece 7 and becomes the sample receiver 4, and the sample can enter the mixing chamber 3 from this portion.
[0036]
Reference numeral 8 denotes a reagent previously placed in the mixing chamber 3 according to the test object as required. The reagent may be put alone, but it may be impregnated with, for example, latex. These are appropriately selected depending on the properties of the reagent and the required amount. In addition, the reagent may be completely fixed in the mixing chamber 3 or may be left unfixed. Depending on the test object, the reagent 8 may not be put in the mixing chamber 3 in advance, but may be put in the mixing chamber 3 together with the sample during the test.
[0037]
In addition, although not shown in figure, you may form an unevenness | corrugation, a groove | channel, etc. in the side which faces the mixing chamber 3 of the cover material 6 or the plate piece 7. FIG. In this way, depending on the test object, the mixing (mixing) may be promoted by the bending restoration operation of the thin plate substrate 1 in some cases.
[0038]
[Example 2]
A plate piece is superimposed on a thin plate substrate, an arbitrary portion of the plate piece is fixed to the substrate, and at least one of the openings where the plate piece is not fixed to the substrate is made larger than the edge of the plate piece. An example in which a cover film is provided as a receiving area, and can cover and seal the entire overlapping surface side of the substrate and the plate piece from above the plate piece will be described with reference to FIGS.
[0039]
Reference numeral 1 denotes a rectangular thin plate substrate made of synthetic resin having a size of 5.5 cm × 9.0 cm and a thickness of 0.1 mm. The size and thickness can be arbitrarily selected according to the test object and the number of test samples as in the first embodiment. As long as it has elasticity, any material can be used as long as it can be bent and restored (bending and stretching operation) and does not affect the chemical reaction. It is.
[0040]
9 is a shallow groove for promoting mixing formed on the thin substrate 1, and the groove 8 has various shapes other than the example shown in the figure as long as the sample can easily enter inside and has a mixing effect. it can. Further, it may not be provided depending on the test object.
[0041]
Reference numeral 10 denotes a spacer fixed between the thin plate substrate 1 and the plate piece 7, and the mixing chamber 3 is formed by these spacers. The spacer 10 has a shape with an open upper side so that a sample or the like can enter the mixing chamber 3 from here. The spacer 10 may be elastic or flexible like the plate side 7, or may not have these properties. In addition to synthetic resin and metal, a material having water absorption may be used. If a water-absorbing material is used, even if there are too many samples, these can be prevented from overflowing from the mixing chamber 3.
[0042]
In the illustrated example, two plate pieces 7 are fixed to the thin plate substrate 1 so that two mixing chambers 3 are provided on the thin plate substrate 1. Any number of the mixing chambers 3 can be selected depending on the test object and the number of specimens, and a plurality of one plate piece 7 can be selected by fixing a plurality of plate pieces 7 to the thin plate substrate 1. Alternatively, the mixing chamber 3 may be formed. Note that at least one of the thin plate substrate 1 and the plate piece 7 is at least a transparent body so that the mixing chamber 3 can be observed from the outside.
[0043]
Since the thin plate substrate 1 is larger than the plate piece 7, the opening of the mixing chamber 3 of the thin plate substrate 1 becomes the sample receiver 4. A sample is placed in this portion, and the sample is allowed to enter the mixing chamber 3 by capillary action. In the mixing chamber 3, a reagent 8 is placed in advance as necessary in the same manner as in the first embodiment. When the reagent 8 is put into the mixing chamber 3, the reagent 8 may be fixed to a part of the spacer 10, or the spacer 10 may be impregnated with a reagent component.
[0044]
Reference numeral 11 denotes a re-adhesive cover film, the upper end of which is fixed to the thin plate substrate 1. The cover film 11 is thin from the top of the plate piece 7 Board The substrate 1 can be sealed, and during storage before the test, the reagent 8 is prevented from evaporating and the like, and the sample is prevented from coming into contact with the outside air during storage after the test, thereby improving the storage stability. The cover film 11 may be provided as necessary, and all the mixing chambers 3 may be closed by one cover film 11, or the cover film 11 may be provided for each mixing chamber 3. Good.
[0045]
In the illustrated example, the thin plate substrate 1 and the plate piece 7 are fixed with the spacer 10 interposed therebetween, but the thin plate substrate 1 and the plate piece 7 may be directly fixed without providing the spacer 10. In addition, the thin plate piece 7 Board The location to be fixed to the substrate 1 can also be selected arbitrarily depending on the test object, for example, it may be fixed to two opposite sides in addition to the three sides or the U shape, or may be fixed to the L shape, or only one side. Or it may be fixed in the form of dots.
[0046]
[Other Examples]
5 to 9 show another embodiment of a mixing test device that can be used in the mixing test method according to the present invention. Each one is suitable for the following tests.
[0047]
FIG. 5 shows a single thin plate substrate 1 with four mixing chambers 3 and a colorimetric standard portion 12 corresponding to the test. In addition, as shown in the figure, the spacer 10 forming the mixing chamber 3 may be partially uneven to promote mixing. Then, different reagents 8 may be previously placed in the mixing chambers 3 in the mixing chambers 3 according to the test contents.
[0048]
Fig. 6 shows an erythrocyte type test. Board Three mixing chambers 3 for blood recipients are arranged on the upper side of the substrate 1, and three colorimetric contrast mixing chambers 13 for blood donors are provided on the lower side so as to correspond to the three mixing chambers 3. Each of the mixing chambers 3 and 13 is covered with a cover member 6 on the back side, and the front side is also closed with a plate piece 7 via a spacer 10. The plate piece 7 has a through hole 14 for sample injection. In the illustrated example, the through-hole 14 is a round hole, but may be any other shape such as a triangle or a quadrangle. The plate piece 7 may be used as the sample receiver 4 without blocking the entire surface and leaving an opening. In addition, if a plurality of through holes 14 are provided in one mixing chamber 3 and are used as air vent holes, injection can be performed quickly depending on the sample.
[0049]
Then, anti-A serum, anti-B serum, and anti-D serum are sequentially placed as reagents 8 at the bottoms of the spacers 10 of the mixing chambers 3 and 13 in a form corresponding to the top and bottom. It is known that blood types of A type, B type, O type, AB type and RH (+) (-) can be identified by examining the reactions of anti-A serum, anti-B serum and anti-D serum. In the illustrated example, the same reagent 8 is put in two places on the left and right sides of the bottom of the spacer 10 for one mixing chamber 3 and 13 for safety. In the above-described example, the mixing chamber 3 is for the blood recipient and the colorimetric object mixing chamber 13 is for the blood donor, but this may be reversed. In any case, as will be described later, it is sufficient that the reactions in the upper and lower stages coincide.
[0050]
In FIG. 7, three reagent chambers 16 (b, b, c) are provided on one thin plate substrate 1, and these reagent chambers a, b, c are respectively communicated with the mixing chamber 4. In addition, a reagent stopper 17 is provided between the reagent chamber A and the reagent chamber C and the mixing chamber. When the reagent stopper 17 is pulled out, the reagent in the reagent chamber A or the reagent chamber C enters the mixing chamber. is there.
[0051]
FIG. 8 shows an example of a vertically long type, in which two opposite sides of the plate piece 7 are directly fixed to the thin plate substrate 1 with an adhesive 15 so as to sandwich the spacer 10 between the thin plate substrates 1. is there. The sample or reagent dropped on the sample receiver 4 enters along the spacer 10 and enters the mixing chamber 3. In the illustrated example, two mixing chambers 3 are shown. However, the number of the mixing chambers 3 can be appropriately selected depending on the test object.
[0052]
FIG. 9 also shows an example of a vertically long type, in which the bottom of the center is rounded from one sample receptacle 4 to promote mixing, and to collect sediment and sediment at an angle. In the reagent chamber 16 (b, b, c, d, e), the reagent chamber a and the reagent 8 can be mixed and sent to the mixing chamber 3, and the reagent chamber c and the reagent 8 can be mixed. The reagent 8 in the reagent chamber can be sent to the mixing chamber 3 alone. In this single mixing chamber 3, six or more types of reagents 8 are pinched from both sides with the thumb and index finger, and various types of reagents 8 are put in advance one after another as necessary to continuously feed them into the mixing chamber 3. Is.
[0053]
FIG. 10 shows a special inspection. One thin plate substrate 1 has three mixing chambers 3. Each mixing chamber 3 is fixedly closed by a dotted line on the back side with a cover material 6, and the front side is also respectively shown. The plate piece 7 is closed. A through hole 14 for sample injection is formed in the upper part of the plate piece 7. The through-hole 14 can have an arbitrary shape. In the illustrated example, a triangular shape, a * shape, and a circular shape are shown from the left. In addition, if a plurality of through holes 14 are provided in one mixing chamber 3 and are used as air vent holes, injection can be performed quickly depending on the sample.
[0054]
Next, the mixing test of the present invention using the above-described mixing test device. Direction A method for implementing the law will be described.
[0055]
[Test Method 1]
A color development test method will be described using the mixed test device shown in FIG.
A sample is dropped into the sample receiver 4 of each mixing chamber 3 and enters the mixing chamber 3 by utilizing capillary action. At this time, the substrate 1 may be bent so that the gap between the mixing chambers 3 is increased. In addition, the reagent is put in each mixing chamber 3 in the same manner.
[0056]
When the sample and the reagent enter each mixing chamber 3, hold the both ends of the thin plate substrate 1 by hand (for example, one end with the thumb and the other end with the middle finger), and bend and stretch the thin plate substrate 1. Operate and mix the reagent and sample. When the bending recovery operation is performed on the thin plate substrate 1, the shape of the mixing chamber 3 is deformed, the gap is increased or decreased, and the mixing (mixing) action is performed. Mixing) is promoted. In addition, the periphery of the spacer 10 serves as an air vent at this time.
[0057]
When the mixing operation is completed and reacts with the reagent to develop a color, the color of each mixing chamber 3 is compared with the color of the colorimetric contrast unit 12 and the result is judged.
[0058]
[Test Method 2]
A mixed test method for examining the blood type of a subject using the mixed test device shown in FIG. 6 will be described.
First, blood collected from a blood recipient is dropped or attached to the sample receiver 4 into the upper three mixing chambers 3 and is allowed to enter the mixing chamber 3 by utilizing capillary action. Similarly, the blood of the blood donor is dropped or applied to the sample receiver 4 in the three colorimetric contrast mixing chambers 13 at the lower stage, and is allowed to enter the mixing chamber 3 by utilizing capillary action.
[0059]
Alternatively, prepare a blood collection tool in the form of a fan made of a set of three strips of paper or resin pieces, put a wound on the earlobe of the recipient and the donor with a needle needle, and receive the blood to be pumped with this blood collection tool Thereafter, it may be opened and inserted into the respective mixing chambers 3 and 13.
[0060]
If the blood in each of the mixing chambers 3 and 13 reacts with each antiserum reagent placed in the substrate 1 or the spacer 10, the result of the mixing chamber 3 serving as the upper blood recipient is used as a colorimetric control serving as the lower blood donor. In contrast to the results in the mixing chamber 13, if the blood type of the recipient and the donor is specified and the aggregation in the upper mixing chamber 3 and the lower colorimetric control mixing chamber 13 match, the blood type is matched. Will be. In the case where there is a cover film, the test result can be easily stored in a medical record or the like for a certain period of time by sealing the whole so as to close the sample receiver 4 and the through hole 14 after the test.
[0061]
【The invention's effect】
As mentioned above, chemical reaction visual mixing test according to the present invention Direction According to the method, an elastic thin plate substrate is provided with a mixing chamber for mixing a reagent and a sample that can be observed from the outside, and after the reagent and the sample are put into the mixing chamber, the substrate is bent and a restoring operation is performed. Since the reagent and the sample were mixed and the result was judged by observing the mixing chamber from the outside, the test can be easily performed anywhere.
[Brief description of the drawings]
FIG. 1 is an exploded perspective view of FIG.
FIG. 2 is a plan view showing a mixing test tool that can be used in the chemical reaction visual mixing test method according to the present invention, which is composed of a substrate, a cover material, and a plate piece.
FIG. 3 is an exploded perspective view of FIG. 4;
FIG. 4 Use of a chemical reaction according to the present invention Can be used in mixed test methods Mixed test equipment so It is a top view which shows what was comprised by two sheets of a board | substrate and a cover material.
FIG. 5 is for visualizing a chemical reaction according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixing test device, and shows the thing for a colorimetric contrast test.
FIG. 6 is for visualizing a chemical reaction according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixing test device, and shows the thing for erythrocyte type | mold test | inspection.
FIG. 7 is for visualizing a chemical reaction according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixing test device, and shows what made it possible to send three reagents independently to one mixing chamber.
FIG. 8 is for visualizing a chemical reaction according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixing test device, and shows what pinched | interposed the water absorbing spacer.
FIG. 9 is a chemical reaction visual inspection according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixing test device, and shows what was made to mix a some reagent in one mixing chamber, or to send it independently.
FIG. 10 is for visualizing a chemical reaction according to the present invention. Can be used in mixed test methods It is a top view which shows the other example of a mixed test device, and shows the thing for special tests.
[Explanation of symbols]
1 Substrate
2 Punching part
3 mixing chamber
4 Sample holder
5 Projections
6 Cover material
7 Plate pieces
8 Reagents
9 groove
10 Spacer
11 Cover film
12 Comparison part
13 Mixing chamber for comparison
14 Through hole
15 Adhesive
16 Reagent room
17 Reagent stopper

Claims (1)

An elastic thin plate substrate is provided with an externally observable mixing chamber for mixing the reagent and sample, and after the reagent and sample are put into the mixing chamber, the thin plate substrate is bent and restored. A chemical reaction visual mixing test method characterized in that a reagent and a sample in a mixing chamber are mixed, and the result is determined by observing the mixing chamber from the outside.

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