JP3674742B2 - Chick salmonella inhibitor for chicks and method for inhibiting chick salmonella using the same - Google Patents

Description

【００１４】
【発明が解決しようとする課題】
上記問題点がヌルミ法が野外で普及するための障害となっており、また使用されても十分な量のＣＥ培養物を雛に投与できないため、ＣＥ培養物が本来有する良好なサルモネラ抑制効果を十分に得られない理由の一つとなっている。
【００１５】
本発明は、特別な装置を必要とせずに、雛の孵化後できるだけ早期に必要量のＣＥ培養物を投与するのに適したタイプの雛用サルモネラ抑制剤及びそれを用いる雛のサルモネラ抑制方法を提供することを目的としている。
【００１６】
【課題を解決するための手段】
本発明者は上記課題を解決すべく鋭意検討した結果、ＣＥ培養物をゲル状に固形して雛に自然摂取させたときは、飲水では１時間で１ｍｌ程度しか飲まない雛でも、１時間で４ｍｌのゲルを摂取させることが可能であり、液体と異なり容器も簡単なものでよく、若齢期の鶏に省力的に投与するのに最適な方法である上に、従来の散霧、そ嚢内直接投与又は飲水希釈投与方法に比して顕著なサルモネラ抑制効果が得られることを見出し、この知見に基づいて本発明を完成するに至った。
【００１７】
本発明はＣＥ培養物つまり成鶏の盲腸内容物、腸管粘膜又は糞便を嫌気培養した未同定菌製剤もしくは同定菌製剤を水媒体中でゲル化能を有する多糖類を用いてゲル状に固形したことを特徴とする雛用ゲル状サルモネラ抑制剤並びに該雛用ゲル状サルモネラ抑制剤を孵化後０〜７日令の雛に投与することを特徴とする雛のサルモネラ抑制方法である。
【００１８】
【発明の実施の形態】
本発明において雛のサルモネラ抑制剤として用いられるＣＥ培養物は、成鶏の盲腸内容物、腸管粘膜又は糞便を嫌気培養した未同定菌製剤もしくは同定菌製剤のいずれであってもよく、通常、鶏の盲腸内容物培養飼料として市販されている製品をそのまま使用する。代表的な製品を商品名で示せば、次のとおりである。
【００１９】
(１) 未同定菌製剤：
「インテクリーン」（商品名、販売元 伊藤忠飼料(株)）
「アヴィガード」（商品名、販売元 バイエル(株)）
「ブロイラクト」（商品名、販売元 藤沢薬品工業(株)）
(２) 同定菌製剤：
「デローチ２９」（商品名、販売元 共立商事(株)）
これらのＣＥ培養物は孵化後０〜７日令の雛に投与するのに適した濃度になるように水で希釈される。希釈倍率は従来の飲水希釈投与法に準じて適用すればよく、例えば、「インテクリーン」を用いる場合は５〜１０倍程度の希釈液が好ましく使用される。
【００２０】
ＣＥ培養物を水媒体中でゲル化させるのに使用される多糖類しては、例えば寒天、カラギーナン、カルボキシメチルセルロース、澱粉、マンナン、ゼラチン、アルギン酸ナトリウム、アラビヤガム、ロウストビーンガム、キサンタンガム、キトサン、グアーガム、ペクチン、アルギン酸プロピルグリコールエステル、アラビノガラクタン、ガティガム、タマリンドシードガム、プルラン、モルホリン脂肪酸塩、カードラン、トラガントガム等が挙げられる。これらの多糖類の中、安価且つ容易に入手し得る点から、特に寒天、澱粉、マンナン、ゼラチンを用いることが望ましい。
【００２１】
ＣＥ培養物を所定濃度になるように水で希釈し、これに攪拌下水溶性多糖類を添加混合し、均一な溶液とし、常温で放置するか、もしくは冷所（例えば冷蔵庫等）に保管することによりゲル状固形物が得られる。または高温で溶解し低温で凝固するゲル化剤（寒天、ゼラチン等）を用いる場合には、ＣＥ培養物を作成するための培地にあらかじめゲル化剤を加えておき、培地を高圧蒸気殺菌後冷却してゲル化直前に種菌を接種し３７℃で培養後、これを常温で放置するか、もしくは冷所（例えば冷蔵庫等）に保管することによってもゲル状固形物が得られる。
【００２２】
ゲル化した場合のゲル強度は概ね２００〜２０００ｇ／ｃｍ²が適当であり、寒天を用いた場合は寒天の種類により異なるが、概ね０．５〜３．０％の濃度に相当する。
【００２３】
このようにゲル化したＣＥ培養物を、飲水量および飼料摂取量の少ない概ね０〜７日令の若令期の雛に投与すると、床面の固形物を嘴で突っついて摂取しようとする雛の遺伝的プログラム（習性）によって短時間にＣＥ培養物の必要量を省力的に摂取させることができる。この際、若令期の雛に投与することが困難であった生菌剤、ワクチン、薬剤、栄養等も必要に応じてＣＥ培養物と共に混合して水溶性多糖類でゲル化し自然摂取させることにより、これらをＣＥ培養物と同時に効率よく雛に投与することもできる。
【００２４】
雛の時期における水分および栄養の補給はその後の生産性にとって極めて重要であり、栄養を投与する場合はグルコース、マンノース、フラクトースなどの単糖類およびこれらのオリゴ体、シュークロースなどの二糖類の糖類等の炭水化物、スキムミルク等の蛋白質、脂質に加えてビタミン、ミネラル等が挙げられる。この方法でグルコースを投与した場合、無処置対照に比してはるかな良好な体重増加が認められる（後記実施例参照）。
【００２５】
本発明によりＣＥ培養物あるいはこれと一緒に若令期の雛に投与しようとするものを混合、ゲル化し自由摂取させる方法は、孵化場および農場のいずれでも実施することができる他、孵化場から農場への雛の輸送中にも実施することができる。
【００２６】
また、ＣＥ培養物が凍結乾燥物である場合は、これに所定量の水溶性多糖類の粉末を配合したゲル化用調製物を用意しておき、孵化場および農場で使用する際に水で希釈しゲル状固形物とし、対象とする雛に投与する方法が採用されるなど、ＣＥ培養物の形状に応じてサルモネラ抑制剤を調製することが好ましい。
【００２７】
【実施例】
本発明をさらに具体的に示すために、以下に実施例を挙げて説明するが、本発明はこれらの実施例に限定されるものではない。
【００２８】
(１) ゲル状ＣＥ培養物の製造
製造例１
１）純水に培地用寒天粉末を６（ｗ／ｖ）％に加え１２１℃、１５分高圧蒸気殺菌後、恒温水槽で４２℃に保持した。
２）ＣＥ培養物「インテクリーン」を３７℃に加温し、これに上記の寒天溶液を等量加え、よく混合した後に直ちにプラスチックシャーレに流し込んで４℃の冷蔵庫に搬入し固化させた。このときの最終寒天濃度は３(ｗ／ｖ)％であった。
【００２９】
製造例２
１）純水に培地用寒天粉末を２(ｗ／ｖ)％、および３(ｗ／ｖ)％に加え１２１℃、１５分高圧蒸気殺菌後、恒温水槽で４２℃に保持した。
２）ＣＥ培養物「インテクリーン」を３７℃に加温し、これに上記の寒天溶液を等量加え、よく混合した後に直ちにプラスチックシャーレに流し込んで４℃の冷蔵庫に搬入し固化させた。このときの最終寒天濃度はそれぞれ１(ｗ／ｖ)％、１．５（ｗ／ｖ）％であり、このときの１（ｗ／ｖ）％製剤のゲル強度は２５０ｇ／ｃｍ²、底部において２３０ｇ／ｃｍ²であった。
３）また対照として純水に培地用寒天粉末を６（ｗ／ｖ）％に加え１２１℃、１５分高圧蒸気殺菌後、恒温水槽で４２℃に保持した溶液と「インテクリーン」の代わりに純水と等量混合し固化させたものも作成した。この対照の最終寒天濃度は３（ｗ／ｖ）％であった。
【００３０】
製造例３
１）純水に培地用寒天粉末を４（ｗ／ｖ）％に加え１２１℃、１５分高圧蒸気殺菌後、恒温水槽で４２℃に保持した。
２）別にグルコース含有液体飼料「アクアエース」(商品名、伊藤忠飼料(株)製）の１２（ｗ／ｖ）％溶液を純水にて作成し３７℃に加温した。
３）「インテクリーン」、寒天溶液および「アクアエース」溶液を表２に示した割合で混合し、直ちにプラスチックシャーレに流し込んで４℃の冷蔵庫に搬入し固化させた。このときの最終寒天濃度は１（ｗ／ｖ）％であった。
【表２】

【００３１】
(２) サルモネラ抑制試験例
試験例１
（１） 方法
１）市販ブロイラーヒナ５０羽を購入し、下記の試験区分、羽数において表３に示した処置を行った。
【表３】

２）３日令で１羽当たり２．６×１０⁴のＳａｌｍｏｎｅｌｌａ Ｔｙｐｈｉｍｕｒｉｕｍを経口投与した。
３）１０日令で盲腸内容物とクロアカスワズを採材し、クロアカスワズについては個体別に定性培養を行った。
４）盲腸内容物については下記文献に従って半定量培養を行い、感染指数と防御指数を算出した。
文献：Ｍｅａｄ，Ｇ．Ｃ．，ｅｔａｌ．（１９９８），Ｊ．Ｐｒｏｔ．，５２：５００〜５０２
【００３２】
▲２▼ 結果
結果は表４に示したように、寒天でゲル状に固化して自由摂取させる方法が最も効果が高く、次いで散霧、そ嚢内直接投与、飲水投与の順であった。
【表４】

【００３３】
▲３▼ 考察
同一量の「インテクリーン」を様々な方法で経口投与したときに、ゲル状に固化して自由摂取させる方法が最も効果が高いことが判った。
【００３４】
試験例２
▲１▼ 方法
１）市販ブロイラーヒナ４０羽を購入し、下記の試験区分、羽数において表５に示した処置を行った。
【表５】

２）３日令で１羽当たり２．６×１０⁴のＳａｌｍｏｎｅｌｌａ Ｔｙｐｈｉｍｕｒｉｕｍを経口投与した。
３）採材、培養、およびデータ処理方法は試験例１と同様である。
【００３５】
▲２▼ 結果
結果は表６に示したように、寒天３％対照ではサルモネラ抑制効果は全く認められなかった。また寒天濃度が低く（１％）ても良好なサルモネラ抑制効果が認められた。
【表６】

【００３６】
▲３▼ 考察
寒天固化製剤の良好な効果は寒天自体の効果ではなく、「インテクリーン」の効果であることが証明された。また効果は寒天濃度に左右されないことが判った。
【００３７】
試験例３
試験例３−１
試験期間：１９９８年１月１３日（０日令）〜１月２０日（７日令）
試験場所：黒磯市青木９１３番地 伊藤忠飼料(株)総合技術研究部内代謝室
供 試 鶏：コッブブロイラー（♂） 各区 ７羽×３反復＝４２羽
処 理：（１） 対照区
入雛と同時に水付けを行い、その後エツケを行った。通常管理。
（２） インテクリーン区［試験区(１)］
水付け前の雛に「インテクリーン」入り寒天ゼリーを投与。投与後は通常管理。
（３） グルコース区
水付け前の雛にグルコース入り寒天ゼリーを投与。投与後は通常管理。
（４） インテ＋グル区［試験区(２)］
水付け前の雛に（「インテクリーン」＋グルコース）入り寒天ゼリー）を投与。投与後は通常管理。
結 果：結果は表７［試験１］に示した。
【００３８】
参考例
試験期間：１９９８年１月１３日（０日令）〜１月２０日（７日令）
試験場所および供試鶏は試験例３−１と同じ
処 理：（１） 対照区
入雛と同時に水付けを行い、その後エツケを行った。通常管理。
（２） 寒天区
水付け前の雛に寒天ゼリーを投与。投与後は通常管理。
結 果：結果は表７［試験２］に示した。
【表７】

【００３９】
試験例３−１について
１）全処理とも寒天の食い付きは良好（３０〜４０分間で完食）。
２）試験終了体重はインテ＋グル区＞グルコース区＞インテクリーン区＞対照区の順となり、全試験区で対照区を上回った。
３）グルコースおよびインテクリーンを組み合わせて投与することにより、単体で投与するとりも効果が大きくなることが確認された。
４）飼料摂取量については、インテ＋グル区＞グルコース区＞対照区＞インテクリーン区の順となり、体重の大きかったインテ＋グル区、グルコース区で多かった。
【００４０】
参考例について
１）寒天区では、寒天の食い付きは良好（３０〜４０分間で完食）。
２）試験終了体重は対照区に比べ、寒天区で２．４ｇ大きく、それに伴い飼料摂取量も寒天区で２．１ｇ多くなった。
３）餌付け前の雛に寒天を投与するとその後の発育が優れることが確認された。
４）寒天ゼリーの水分含量が高いことから、ゼリー給与により餌付け時の水付け作業の省略、または簡略化が可能である。
【００４１】
【発明の効果】
以上説明したように、本発明は飲水量および飼料摂取量の少ない概ね０〜７日令の若令期の雛に、短時間にＣＥ培養物の必要量を省力的に投与するに適した雛用ゲル状サルモネラ抑制剤およびそれを用いる雛のサルモネラ抑制方法に関するもので、従来行われていた散霧、そ嚢内直接投与又は飲水希釈投与方法に比して顕著なサルモネラ抑制効果を奏する。[0001]
BACKGROUND OF THE INVENTION
The present invention is a gel-like salmonella inhibitor for chicks that is suitable for administering chick cecal contents, intestinal mucosa or stool anaerobically cultured unidentified bacteria preparation or identified bacteria preparation obtained by culturing the same. And a method for suppressing chick Salmonella using the same.
[0002]
[Prior art]
Recently, the occurrence of mass food poisoning due to pathogenic E. coli O-157, H7 has become a social problem. According to the food poisoning statistics for each etiological agent in 1996, both the number of Salmonella food poisoning and the number of patients were found to be pathogenic E. coli O-157, H7. It is far above those by and occupies the first place. One of the main causes is frequent food poisoning due to Salmonella Enteritidis (hereinafter abbreviated as “SE”) in chicken eggs.
[0003]
When a laying hen is infected with SE, it is distributed in the ovaries and fallopian tubes and enters the hen's eggs. Therefore, at the stage where the chicken lays eggs, the inside of the egg is already contaminated with SE (contamination with In-Egg). Eggs that have been contaminated with In-Egg cannot be distinguished from appearance by appearance. Contamination of eggs inside the eggs is not possible even during the washing process at the GP Center (Grading and Packaging Center, a factory that cleans eggs and sorts them according to size). Cannot be removed.
[0004]
Even if the flock is contaminated with SE, the rate at which SE-contaminated eggs are produced is as low as about 0.01 to 1%. However, SE-contaminated eggs happen to be used during cooking, and due to insufficient heating, SE However, if the storage temperature of the cooked product is not appropriate, SE may proliferate again and increase the number of bacteria to cause food poisoning, which may cause food poisoning. In particular, food poisoning occurs in low-resistant infants, elderly people, or sick people even with a small number of bacteria compared to healthy adults. Therefore, food poisoning due to SE may occur at home, but collective food poisoning that occurs in food service facilities such as restaurant industry facilities, schools, hospitals, and military forces causes extremely serious social problems.
[0005]
In order to ensure the hygienic quality of eggs and to reduce Salmonella food poisoning, it is necessary to take measures at all stages of production distribution from the farm to the table. However, when considering SE, the main point is to maintain the flock in a Salmonella-free situation on the farm, or to reduce the contamination rate.
[0006]
It is known that chickens are extremely sensitive to Salmonella, especially at a young age, and in fact, if a small number of Salmonella are infected in chicks, they are eradicated in large quantities. In modern poultry farming, chicks are produced in the hatchery, the chicks do not have the opportunity to come into contact with their parent chickens, and are thoroughly sterilized at each stage to prevent the transmission of disease from the seed chickens. The main reason for the above phenomenon is that the chicks cannot inherit the useful intestinal flora necessary for the defense against intestinal pathogens from the parent chicken. Therefore, it is said that a long time of 6 weeks or more is required to establish the intestinal microflora necessary for the defense against infection of Salmonella normally bred outdoors.
[0007]
In this way, only poultry including chickens are produced and raised without contact with their parents, and if the intestinal flora is immature, infection with Salmonella in younger ages, A high degree of contamination occurs, which is believed to affect the rate of Salmonella contamination in later flocks.
[0008]
Regarding effective methods for protecting Salmonella infection, E. Nurmi and M. Rantala forcibly establish the intestinal flora of adult chickens in chicks, and if Salmonella is orally infected later, And was found in the scientific journal “Nature” in 1973 [E. Nurmi and M. Rantala; New aspects of Salmonella. inspection in broiler industry. Nature (London) 241: 210-211 (1973)]. After that, an enormous amount of additional testing was conducted, and the effects and reproducibility of this phenomenon were confirmed. The phenomenon and the technology using this phenomenon took the name of the reporter as the Nurmi method or the Competitive Exclusion method. being called.
[0009]
Intestinal flora to be administered to chicks is widely administered by anaerobic culture of cecal contents, intestinal mucosa or feces of adult chickens. It is called undefined culture because it cannot be clearly identified. In addition, the bacterial species and the number of bacteria contained in the unidentified bacterial preparations are continuously produced for a long period of time, and a certain number of bacteria are produced. Things are also used. These are referred to as identified bacterial preparations because they contain known bacterial species and numbers, and unidentified bacterial preparations and identified bacterial preparations are collectively referred to as “CE cultures”.
[0010]
In order to obtain a good Salmonella inhibitory effect of the CE culture, it is necessary to administer the required amount of the CE culture as soon as possible after hatching of the chicks. Therefore, it is best to orally administer one bird at a time, as is done in the laboratory, but this method is not practical because it is laborious and time consuming, that is, costly. In the field, mainly spraying eggs for hatching and fogging for chicks at the hatchery, and drinking water on the farm are administered.
[0011]
However, for implementation in the hatchery, isolation from non-administered flocks is difficult, spraying equipment is required, odors peculiar to CE cultures become a problem, chicks get wet, waste that is not consumed by chicks There are many problems. Also, regarding farm implementation, especially in large-scale poultry houses, the amount of water entering the drinking line is large, whereas the amount of water consumed by chicks is very small, so a certain amount of CE culture is ingested for a certain period of time. In order to achieve this, there are many cases in which a large number of CE cultures of several minutes or more must be prepared. In addition, the operation of entering chicks becomes more complicated and labor-intensive. There are problems such as being greatly influenced by conditions. Furthermore, it should be noted that the quality of chicks has the most influence on the amount of water consumed, and the amount of water consumed within a certain period of time after entering the chick varies greatly depending on the elapsed time since hatching.
[0012]
Table 1 shows the results of a comparative study of the amount of water consumed by chicks on the day of hatching and chicks that have passed one day. The amount of water consumed up to 6 hours after entering the chicks is more than doubled. It is very difficult for all chicks to drink water uniformly with the drinking water administration method, and even after 5 hours from entering the chicks, about 90% of the chicks were drinking. Chicken egg meat information ”Salmonella countermeasures by CE method (Nurumi method) (February 10, 1995 issue)].
[0013]
[Table 1]

[0014]
[Problems to be solved by the invention]
The above-mentioned problem is an obstacle for the spread of the Nurumi method in the field, and even if it is used, a sufficient amount of CE culture cannot be administered to chicks. This is one of the reasons why it cannot be obtained sufficiently.
[0015]
The present invention provides a chick salmonella inhibitor of a type suitable for administering a necessary amount of CE culture as soon as possible after hatching of chicks without requiring a special device, and a method for suppressing chick salmonella using the same. It is intended to provide.
[0016]
[Means for Solving the Problems]
As a result of intensive studies to solve the above-mentioned problems, the present inventor found that when CE culture was solidified in a gel and allowed to be naturally ingested by chicks, even chicks that drink only about 1 ml in 1 hour can be consumed in 1 hour. It is possible to ingest 4 ml of gel, and unlike a liquid, it can be a simple container and is the best method for labor-saving administration to young chickens. It has been found that a remarkable salmonella inhibitory effect can be obtained as compared with the direct administration in the sac or the drinking water dilution administration method, and the present invention has been completed based on this finding.
[0017]
In the present invention, a CE culture, that is, an unidentified bacterial preparation obtained by anaerobically culturing caecal contents, intestinal mucosa or feces of an adult chicken, or an identified bacterial preparation was solidified in a gel using a polysaccharide having gelling ability in an aqueous medium. A chick salmonella inhibitor characterized by the above, and a chick salmonella inhibitor comprising administering the chick gel salmonella inhibitor to chicks aged 0 to 7 days after hatching.
[0018]
DETAILED DESCRIPTION OF THE INVENTION
The CE culture used as a chick Salmonella inhibitor in the present invention may be any of an unidentified bacterial preparation or an identified bacterial preparation obtained by anaerobically culturing the cecal contents, intestinal mucosa or feces of an adult chicken. The product marketed as cecal content culture feed is used as it is. Representative products are indicated by trade names as follows.
[0019]
(1) Unidentified bacteria preparation:
"INTECLEAN" (trade name, distributor ITOCHU FEDERATION CO., LTD.)
“Avigard” (trade name, distributor: Bayer Co., Ltd.)
"Braulacto" (trade name, distributor: Fujisawa Pharmaceutical Co., Ltd.)
(2) Identified bacteria preparation:
“Deloch 29” (trade name, distributor Kyoritsu Shoji Co., Ltd.)
These CE cultures are diluted with water to a concentration suitable for administration to 0-7 day old chicks after hatching. The dilution ratio may be applied in accordance with the conventional drinking water dilution administration method. For example, when “INTECLEAN” is used, a diluted solution of about 5 to 10 times is preferably used.
[0020]
The polysaccharides used to gel the CE culture in an aqueous medium include, for example, agar, carrageenan, carboxymethylcellulose, starch, mannan, gelatin, sodium alginate, arabic gum, roast bean gum, xanthan gum, chitosan, Examples include guar gum, pectin, propyl glycolic alginate, arabinogalactan, gati gum, tamarind seed gum, pullulan, morpholine fatty acid salt, curdlan, and tragacanth gum. Of these polysaccharides, agar, starch, mannan, and gelatin are particularly preferable because they are inexpensive and easily available.
[0021]
Dilute the CE culture with water to a predetermined concentration, add and mix water-soluble polysaccharides with stirring to make a uniform solution, and leave it at room temperature or store it in a cool place (for example, a refrigerator). To obtain a gel-like solid. Alternatively, when using a gelling agent (agar, gelatin, etc.) that dissolves at high temperature and solidifies at low temperature, the gelling agent is added in advance to the medium for preparing the CE culture, and the medium is cooled after high-pressure steam sterilization. Then, after inoculating the inoculum immediately before gelation and culturing at 37 ° C., it is allowed to stand at room temperature, or stored in a cold place (for example, a refrigerator) to obtain a gel-like solid.
[0022]
The gel strength when gelled is approximately 200 to 2000 g / cm ² , and when agar is used, it varies depending on the type of agar, but corresponds to a concentration of approximately 0.5 to 3.0%.
[0023]
When the gelled CE culture is administered to young chicks of approximately 0 to 7 days of age with a small amount of drinking water and feed intake, the chicks trying to ingest the solid matter on the floor with a broom. Thus, the necessary amount of CE culture can be saved labor-saving in a short time. At this time, live bacteria, vaccines, drugs, nutrients, etc. that were difficult to administer to young chicks should be mixed with CE culture as needed, gelled with water-soluble polysaccharides and naturally ingested. Thus, these can be efficiently administered to chicks simultaneously with the CE culture.
[0024]
Hydration and nutritional supplementation during the chick season is extremely important for subsequent productivity. When nutrition is administered, monosaccharides such as glucose, mannose, and fructose, and oligosaccharides such as these, saccharides of disaccharides such as sucrose, etc. In addition to carbohydrates, proteins such as skim milk, lipids, vitamins, minerals and the like. When glucose is administered in this manner, a much better weight gain is observed compared to untreated controls (see Examples below).
[0025]
According to the present invention, the method for mixing, gelling, and freely ingesting CE cultures or those to be administered to young chicks together with this can be carried out either in the hatchery or on the farm. This can also be done while transporting chicks to the farm.
[0026]
If the CE culture is a lyophilized product, prepare a gelling preparation containing a predetermined amount of water-soluble polysaccharide powder and use it with water when used in hatcheries and farms. It is preferable to prepare a Salmonella inhibitor according to the shape of the CE culture, such as a method of diluting it into a gel-like solid and administering it to the target chick.
[0027]
【Example】
In order to show the present invention more specifically, the present invention will be described below with reference to examples. However, the present invention is not limited to these examples.
[0028]
(1) Production and production example 1 of gel CE culture
1) Agar powder for medium was added to 6 (w / v)% in pure water, and after autoclaving at 121 ° C. for 15 minutes, it was kept at 42 ° C. in a constant temperature water bath.
2) The CE culture “INTECLEAN” was warmed to 37 ° C., and an equal amount of the above-mentioned agar solution was added thereto, mixed well, then immediately poured into a plastic petri dish, carried into a 4 ° C. refrigerator, and solidified. The final agar concentration at this time was 3 (w / v)%.
[0029]
Production Example 2
1) Agar powder for medium was added to pure water at 2 (w / v)% and 3 (w / v)%, and after autoclaving at 121 ° C. for 15 minutes, it was kept at 42 ° C. in a constant temperature water bath.
2) The CE culture “INTECLEAN” was warmed to 37 ° C., and an equal amount of the above-mentioned agar solution was added thereto, mixed well, then immediately poured into a plastic petri dish, carried into a 4 ° C. refrigerator, and solidified. The final agar concentrations at this time are 1 (w / v)% and 1.5 (w / v)%, respectively, and the gel strength of the 1 (w / v)% formulation at this time is 250 g / cm ^{2 at} the bottom. It was 230 g / cm ² .
3) In addition, as a control, agar powder for medium was added to pure water in 6 (w / v)%, and after pasteurization at 121 ° C for 15 minutes under high-pressure steam sterilization, the solution was kept at 42 ° C in a constant temperature bath and pure instead of "integrin" An equal volume of water and solidified were also prepared. The final agar concentration of this control was 3 (w / v)%.
[0030]
Production Example 3
1) Agar powder for culture medium was added to pure water in 4 (w / v)%, 121 ° C., 15 minutes high-pressure steam sterilization, and then maintained at 42 ° C. in a constant temperature water bath.
2) Separately, a 12 (w / v)% solution of glucose-containing liquid feed “Aqua Ace” (trade name, manufactured by ITOCHU FEED Co., Ltd.) was prepared with pure water and heated to 37 ° C.
3) “Integre Clean”, agar solution and “Aqua Ace” solution were mixed in the proportions shown in Table 2, immediately poured into a plastic petri dish, carried into a 4 ° C. refrigerator and solidified. The final agar concentration at this time was 1 (w / v)%.
[Table 2]

[0031]
(2) Salmonella suppression test example Test example 1
(1) Method 1) 50 commercially available broiler chicks were purchased, and the treatments shown in Table 3 were performed in the following test categories and number of wings.
[Table 3]

2) 2.6 × 10 ⁴ Salmonella Typhimurium per bird was orally administered at the age of 3 days.
3) The cecum contents and cloacaswaz were sampled at 10 days of age, and qualitative culture of croacaswaz was performed on an individual basis.
4) About the cecum contents, semi-quantitative culture was performed according to the following literature, and the infection index and the protection index were calculated.
Literature: Mead, G .; C. Et al. (1998), J. et al. Prot. 52: 500-502
[0032]
(2) As a result, as shown in Table 4, the method of solidifying in gel form with agar and taking it freely is the most effective, followed by spraying, direct administration in the sac, and drinking water.
[Table 4]

[0033]
(3) Discussion It was found that when the same amount of “INTECLEAN” was orally administered by various methods, the method of solidifying in a gel state and allowing free intake was the most effective.
[0034]
Test example 2
(1) Method 1) 40 commercially available broiler chicks were purchased, and the treatments shown in Table 5 were carried out in the following test categories and numbers.
[Table 5]

2) 2.6 × 10 ⁴ Salmonella Typhimurium per bird was orally administered at the age of 3 days.
3) The sampling, culture and data processing methods are the same as in Test Example 1.
[0035]
(2) As a result, as shown in Table 6, the Salmonella inhibitory effect was not recognized at all in the agar 3% control. Moreover, even if the agar concentration was low (1%), a good salmonella inhibitory effect was observed.
[Table 6]

[0036]
(3) Discussion It was proved that the good effect of the agar solidified preparation was not the effect of agar itself, but the effect of “integrin”. It was also found that the effect was not affected by the agar concentration.
[0037]
Test example 3
Test Example 3-1
Test period: January 13, 1998 (day 0) to January 20 (day 7)
Test location: 913, Aoki, Kuroiso City Itochu Feed Co., Ltd. Metabolism Laboratory, Research Division, Chickens: Cobb broiler (♂) Each ward 7 birds x 3 repeats = 42 treatments: (1) Control ward
Watering was performed at the same time as Irihina, and then Etsuke was performed. Normal management.
(2) Inteclean District [Test Zone (1)]
Agar jelly with IntelliClean is administered to chicks before watering. Normal administration after administration.
(3) Glucose section
Glucose-containing agar jelly was administered to chicks before watering. Normal administration after administration.
(4) Inte + Guru [Test Zone (2)]
Agar jelly ("integrin" + glucose) in chicks before watering. Normal administration after administration.
Results: The results are shown in Table 7 [Test 1].
[0038]
Reference Example Test Period: January 13, 1998 (0-day decree) to January 20 (7-day decree)
Test location and test chicken are the same as Test Example 3-1. Treatment: (1) Control group
Watering was performed at the same time as Irihina, and then Etsuke was performed. Normal management.
(2) Agar
Agar jelly was administered to chicks before watering. Normal administration after administration.
Results: The results are shown in Table 7 [Test 2] .
[Table 7]

[0039]
Regarding Test Example 3-1, 1) Agar biting is good in all treatments (completed in 30 to 40 minutes).
2) Test body weight was in the order of inte + guru group> glucose group> inteclean group> control group, and exceeded the control group in all test groups.
3) It has been confirmed that administration of glucose and Integrin in combination improves the effect of administration alone.
4) The feed intake was in the order of inte + guru group> glucose group> control group> integrin group, and was large in the inte + glu group and glucose group, which had a large body weight.
[0040]
About Reference Examples 1) In the agar ward, the agar bite is good (completed in 30 to 40 minutes).
2) The weight at the end of the test was 2.4 g larger in the agar area than in the control area, and the feed intake increased 2.1 g in the agar area accordingly .
3) It was confirmed that the subsequent growth was excellent when agar was administered to chicks before feeding.
4) Since the water content of agar jelly is high, the watering operation during feeding can be omitted or simplified by feeding jelly.
[0041]
【The invention's effect】
As described above, the present invention is a chick suitable for labor-saving administration of the required amount of CE culture in a short time to young chicks of approximately 0 to 7 days old with a small amount of drinking water and feed intake. The present invention relates to a gel-like salmonella inhibitor and a method for suppressing chick salmonella using the same, and has a remarkable salmonella inhibitory effect as compared to conventional spraying, intracapsular direct administration or drinking water dilution administration methods.

Claims (5)

  For chicks characterized in that an unidentified bacterial preparation or an identified bacterial preparation obtained by anaerobically culturing adult caecal contents, intestinal mucosa or stool was solidified into a gel using a polysaccharide having gelling ability in an aqueous medium Gel salmonella inhibitor.   The polysaccharide having gelling ability is agar, carrageenan, carboxymethyl cellulose, starch, mannan, gelatin, sodium alginate, arabic gum, roast bean gum, xanthan gum, chitosan, guar gum, pectin, propyl glycolate alginate, arabinogalactan, gati gum, The gel-like salmonella inhibitor for chicks according to claim 1, which is at least one of tamarind seed gum, pullulan, morpholine fatty acid salt, curdlan and tragacanth gum.   Furthermore, the gelatinous salmonella inhibitor for chicks of Claim 1 which contains at least 1 sort (s) of a carbohydrate, a protein, a vitamin, and a mineral. Chicks for Salmonella inhibitor according to any one of claims 1 to 3, the gel strength of the gelled solid is in the range of 200 to 2000 g / cm ^2.   A method for suppressing chick salmonella, comprising administering the chick Salmonella inhibitor according to any one of claims 1 to 4 to chicks aged 0 to 7 days after hatching.