JP3550806B2 - Centrifuge rotor - Google Patents

Description

[0001]
[Industrial applications]
The present invention relates to a rotating body of a centrifuge that separates a sample using centrifugal force.
[0002]
[Prior art]
Conventionally, there is a method of culturing cells and the like and collecting the cells by directly centrifuging a microplate 4 having many holes used for culturing. In order to set the microplate 4 in the centrifugation, a swing type rotor is used as shown in FIG. 3, and a centrifugal force is applied to the bottom of each hole. In FIG. 3, the left half shows the stopped state and the right half shows the rotated state.
[0003]
[Problems to be solved by the invention]
Some microplates have different hole bottom shapes depending on the purpose. U- and V-bottoms tend to collect sediment when centrifuged, but flat bottoms have a disadvantage that they tend to precipitate uniformly at the bottom, making it difficult to collect. In addition, since the sample was precipitated on the entire bottom, it was difficult to prevent the precipitated portion from being sucked when aspirating the supernatant with a pipette or the like. When performing automation or the like, it is difficult to determine the height of the pipette, which is a particularly obstructive step.
[0004]
An object of the present invention is to solve the above-mentioned problems, to increase the concentration of the precipitate even in a flat-bottomed microplate, and to further simplify the work of sucking the supernatant.
[0005]
[Means for Solving the Problems]
In order to achieve the above object, the swing of the microplate bucket can be stopped halfway. In addition, this is achieved by shifting the position of the fulcrum swinging from the position of the center of gravity of the bucket so that the position at the time of swinging becomes a certain angle with respect to the microplate. Further, since the line connecting the fulcrum and the center of gravity is the direction of the centrifugal force, the centrifugal force is applied at a predetermined angle to the microplate as a result.
[0006]
[Action]
With the rotor and the bucket configured as described above, the direction of the centrifugal force applied to the microplate and the direction of the hole have an angle, so that the precipitate can be efficiently collected at a part of the corner of the hole bottom. In addition, since a surface without a precipitate comes out at the bottom of the hole, the suction of the supernatant works so that the suction can be easily performed by aiming at the place without the precipitate.
[0007]
【Example】
One embodiment of a rotor for a centrifuge according to the present invention will be described with reference to FIG. In FIG. 1, the left side of the rotating shaft 8 shows a state where the rotor is stopped, and the right side shows a state of rotating. A bucket 2 on which a microplate 4 is placed is mounted on a body 1 which rotates by receiving a rotational driving force of a motor 7, and the bucket 2 swings by a centrifugal force with a pin 3 applied to the body 1 as a fulcrum. The bucket 2 is supported by a stopper 6 so that the microplate 4 is horizontal while the rotor is stopped. As shown in the left half of FIG. 1, the center of gravity 5 of the bucket 2 is shifted from below the pins 3 vertically when the microplate 4 is in a horizontal state. That is, the line connecting the center of gravity 5 and the pin 3 has an angle of θ with the direction of gravity 10. As the rotor rotates, the bucket 2 swings until the line connecting the center of gravity 5 and the pin 3 is in the horizontal direction, that is, in the centrifugal direction 9. Therefore, the hole direction of the microplate 4 is inclined by the angle θ with respect to the centrifugal force 9. The sample contained in the microplate 4 sediments in the centrifugal direction 9 and sediments at the corner of the bottom of the hole. Unlike a conventional rotor, which precipitates over the entire hole bottom unlike a conventional rotor, the precipitate 12 is located only on a part of the hole bottom. It can be done easily without sucking.
[0008]
【The invention's effect】
According to the present invention, in centrifugation using a microplate, the precipitate can be easily collected at a part of the bottom, so that the work of sucking the supernatant can be easily performed, and automation can be easily performed. . Further, when applied to a centrifuge tube other than a microplate, a function as an angle rotor can be achieved by using a swing rotor. That is, the sedimentation distance is shorter than that of a normal swing rotor, so that the centrifugation time can be shortened.
[Brief description of the drawings]
FIG. 1 is a sectional view showing an embodiment of a centrifuge rotor according to the present invention.
FIG. 2 is an enlarged view of a portion A in FIG.
FIG. 3 is a sectional view showing a conventional rotor for a centrifuge.
[Explanation of symbols]
1 is a rotor body, 2 is a bucket, 3 is a pin, 4 is a microplate, 5 is a center of gravity of the bucket, 6 is a stopper, 7 is a drive motor, 8 is a rotating shaft, 9 is a centrifugal force direction, 10 is a gravity direction, 11 Is a sample supernatant, 12 is a precipitate, 13 is a rotor body, and 14 is a bucket.

Claims (1)

A motor, a rotating shaft for transmitting the rotational driving force of the motor to the body, a microplate having a number of holes for accommodating a sample , and further having a flat bottom shape with the bottom of the hole, In a centrifuge rotor comprising a bucket to be stored and a pin for swingably supporting the bucket with respect to the body, the rotor is provided on the body, and the microplate is horizontal while the motor is stopped. A rotor for a centrifugal separator , comprising a stopper for supporting a bucket, wherein the bucket has a center of gravity located outside the pin when the microplate is in a horizontal state .

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