JP3467904B2 - Decomposition method of nitrile rubber - Google Patents
Decomposition method of nitrile rubberInfo
- Publication number
- JP3467904B2 JP3467904B2 JP11236895A JP11236895A JP3467904B2 JP 3467904 B2 JP3467904 B2 JP 3467904B2 JP 11236895 A JP11236895 A JP 11236895A JP 11236895 A JP11236895 A JP 11236895A JP 3467904 B2 JP3467904 B2 JP 3467904B2
- Authority
- JP
- Japan
- Prior art keywords
- nitrile rubber
- hydrophilic group
- sulfuric acid
- rubber
- concentrated sulfuric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Compositions Of Macromolecular Compounds (AREA)
- Biological Depolymerization Polymers (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
- Separation, Recovery Or Treatment Of Waste Materials Containing Plastics (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、ニトリルゴムの分解方
法に関する。更に詳しくは、微生物によるニトリルゴム
の分解方法に関する。FIELD OF THE INVENTION The present invention relates to a method for decomposing nitrile rubber. More specifically, it relates to a method for degrading nitrile rubber by a microorganism.
【０００２】[0002]
【従来の技術】従来、微生物によるゴムの分解法とし
て、天然ゴムを対象とするものが数多く提案されている
が（微工研ニュース１９８７年７月号第１〜４頁、日本
農芸化学会誌第６５巻第９８１〜６頁、１９９１年な
ど）、合成ゴムを対象としたものは未だ知られてはいな
い。もっとも、キサントモナス属に属する微生物を用
い、イソプレン系ゴムを分解することが特公平６―８３
６５号公報に記載されているが、合成イソプレンゴムは
本来天然ゴムと骨格を同じくするものであって、この公
報にも微生物分解の対象とされるイソプレン系ゴムには
天然ゴムおよび合成イソプレンゴムを含むと記載されて
おり、完全に人工的に合成された合成ゴムとはいえない
面を有している。2. Description of the Related Art Conventionally, as a method for decomposing rubber by microorganisms, many methods for natural rubber have been proposed (Mikken News, July 1987, pages 1 to 4, Journal of the Japanese Society of Agricultural Chemistry). 65, 981-6, 1991, etc.), synthetic rubber is not yet known. However, using a microorganism belonging to the genus Xanthomonas to decompose isoprene-based rubber is disclosed in JP-B-6-83.
As described in Japanese Patent Publication No. 65, the synthetic isoprene rubber originally has the same skeleton as that of natural rubber, and in this publication, natural rubber and synthetic isoprene rubber are used as isoprene-based rubbers that are targeted for microbial decomposition. It is described as containing, and has a surface that cannot be said to be a synthetic rubber that is completely artificially synthesized.
【０００３】[0003]
【発明が解決しようとする課題】本発明の目的は、合成
ゴムであるニトリルゴム（ＮＢＲ）を微生物により分解
する方法を提供することにある。SUMMARY OF THE INVENTION An object of the present invention is to provide a method for degrading synthetic rubber nitrile rubber (NBR) by microorganisms.
【０００４】[0004]
【課題を解決するための手段】かかる本発明の目的は、
バチルス属に属し、濃硫酸浸漬処理による親水性基導入
ニトリルゴム分解能を有する微生物を、濃硫酸浸漬処理
による親水性基導入ニトリルゴムを含有する培地に添加
し、培養することによりニトリルゴムを分解する方法に
よって達成される。また、このような微生物を培養し、
得られた培養菌体、培養ロ液またはこれらの処理物を、
濃硫酸浸漬処理による親水性基導入ニトリルゴムと接触
させることによっても、ニトリルゴムを分解させること
ができる。ニトリルゴムは、それの微生物分解性を高め
るため、そこに親水性基を導入した上で、微生物による
分解に付される。ニトリルゴムへの親水性基の導入は、
ニトリルゴムを濃硫酸中に、室温乃至約２５℃の温度で
約１５〜３０時間程度浸漬することにより行われる。そ
の後、浸漬液中から取り出され、水洗および乾燥（約４
０〜５０℃）される。このような親水性基含有ニトリル
ゴム分解能を有する微生物としては、バチルス属に属す
る微生物が用いられる。バチルス属に属する微生物とし
ては、バチルスｓｐ．Ｎｏ．Ｓ３Ａ（ＦＥＲＭＰ―１３
５７３）などが用いられる。 The object of the present invention is as follows.
Microorganisms belonging to the genus Bacillus and capable of degrading hydrophilic group-introduced nitrile rubber by immersion in concentrated sulfuric acid are treated with concentrated sulfuric acid.
It was added to the medium containing the hydrophilic group introduced nitrile rubber due to be achieved by the method of decomposing the nitrile rubber by culturing. Also, culturing such microorganisms,
The obtained cultured bacterial cells, culture solution or a processed product of these,
The nitrile rubber can also be decomposed by bringing it into contact with a hydrophilic group-introduced nitrile rubber by a concentrated sulfuric acid immersion treatment . Nitrile rubber is subjected to microbial degradation after introducing hydrophilic groups into it in order to enhance its microbial degradability. Introduction of hydrophilic groups to nitrile rubber
It is carried out by immersing the nitrile rubber in concentrated sulfuric acid at a temperature of room temperature to about 25 ° C. for about 15 to 30 hours. Then, it is taken out from the immersion liquid, washed with water and dried (about 4
0 to 50 ° C.). A microorganism belonging to the genus Bacillus is used as the microorganism having such a hydrophilic group-containing nitrile rubber decomposing ability. Microorganisms belonging to the genus Bacillus include Bacillus sp. No. S3A (FERMP-13
573) and the like are used.
【０００５】バチルスｓｐ．Ｎｏ．Ｓ３Ａは静岡県相良
町の土壌から分離されたものであり、それの培養は、任
意の培地を用い、３７℃の振とう条件下で１〜１０日間
程度行われる。具体的には、Ｌ―ブイヨン（１％トリプ
トン、０．５％酵母エキス、０．５％ＮａＣｌ、殺菌前
のｐＨ７．０）３ｍｌを試験管に入れ、これに採取した
土壌を入れ、３７℃で２４時間振とう培養した。 Bacillus sp. No. S3A is isolated from the soil of Sagara-cho, Shizuoka Prefecture, and the culture thereof is carried out using any medium under shaking conditions at 37 ° C. for about 1 to 10 days. Specifically, 3 ml of L-broth (1% tryptone, 0.5% yeast extract, 0.5% NaCl, pH 7.0 before sterilization) was placed in a test tube, and the soil collected was placed in it at 37 ° C. The cells were shake-cultured for 24 hours.
【０００６】これは、下記の如き菌学的性質を有する。
Ａ．形態
（１）細胞の形桿菌
大きさ０．５×１μｍ
（２）運動性あり
（３）胞子形成あり
（４）グラム染色性陽性
Ｂ．培地における成育状態
肉汁寒天平板培養褐色、隆起あり
Ｃ．生理学的性質
（１）硝酸塩の還元あり
（２）ＶＰテストなし
（３）インドールの生成なし
（４）硫化水素の生成なし
（５）クエン酸の利用あり
（６）色素の生成なし
（７）ウレアーゼの生産なし
（８）オキシダーゼ活性あり
（９）リジンの分解なし
（１０）オルニチンの分解なし
（１１）アルギニンの分解あり
（１２）生育の範囲ｐＨ４〜９
温度３０〜４０℃
（１３）酸素に対する態度通性嫌気性
（１４）糖類からの酸の生成
グルコース＋
マンニット−
アドニット−
アラビノース−
イノシット−
ラムノース−
ソルビット−
麦芽糖−
白糖−
培地：ペプトン２ｇ、ＮａＣｌ５ｇ、Ｋ２ＨＰＯ４０．
３ｇ、炭水化物１０ｇ、
ブロムチモールブルー０．０８ｇ、寒天１５ｇ、蒸留水
１００ｍｌ
（ｐＨ７．１）
添加濃度：１％[0006] It has the following mycological properties. A. Morphology (1) Bacillus size of cells 0.5 × 1 μm (2) Motility (3) Spore formation (4) Gram stain positive B. Growth state in medium Broth agar plate culture brown, with ridge C. Physiological properties (1) Nitrate reduction (2) No VP test (3) No indole formation (4) No hydrogen sulfide formation (5) Citric acid utilization (6) No pigment formation (7) Urease No production of (8) Oxidase activity (9) No decomposition of lysine (10) No decomposition of ornithine (11) Decomposition of arginine (12) Growth range pH 4-9 Temperature 30-40 ° C (13) Attitude toward oxygen Acid formation from facultative anaerobic (14) sugars Glucose + mannitol-adnitol-arabinose-inosit-rhamnose-sorbit-maltose-sucrose-medium: peptone 2 g, NaCl 5 g, K 2 HPO 4 0.
3 g, carbohydrate 10 g, bromthymol blue 0.08 g, agar 15 g, distilled water 100 ml (pH 7.1) Addition concentration: 1%
【０００７】以上の菌学的性質に基づいて、この菌をＭ
ａｎｕａｌｏｆＤｅｔｅｒｍｉｎａｔｉｖｅＢａｃｔｅ
ｒｉｏ―ｌｏｇｙ第９版より検索した結果、バチルス属
に属する菌であることを確認した。Based on the above mycological properties,
anualofDeterminativeBacte
As a result of searching from the 9th edition of rio-logy, it was confirmed to be a bacterium belonging to the genus Bacillus.
【０００８】[0008]
【実施例】この菌を用いてのニトリルゴムの分解は、例
えば１０ｍｇのニトリルゴム（日本ゼオン製品ゼットポ
ール１０１０）を１０ｍｌの３６Ｎ濃硫酸中に一晩放置
した後、取り出して純水で３回洗浄し、乾燥したゴム片
０．５ｇを、３ｍｌのＬ―ｂｒｏｔｈ培地（１％トリプ
トン、０．５％酵母エキスおよび０．５％ＮａＣｌを含
み、殺菌前のｐＨは７．０）に加え、更にＬ―ｂｒｏｔ
ｈ培地で前培養しておいたバチルスｓｐ．Ｎｏ．Ｓ３Ａ
（ＦＥＲＭＰ―１３５７３）を含む培養液０．５ｍｌを
Ｌ―ｂｒｏｔｈ培地１０ｍｌに添加し、振とう条件下に
３０℃で２週間培養することにより行われる。[Examples] Decomposition of nitrile rubber using this bacterium was carried out, for example, by leaving 10 mg of nitrile rubber (Zeon Pall 1010 manufactured by Nippon Zeon Co., Ltd.) in 10 ml of 36N concentrated sulfuric acid overnight, taking it out, and then using pure water three times. 0.5 g of washed and dried rubber pieces was added to 3 ml of L-broth medium (containing 1% tryptone, 0.5% yeast extract and 0.5% NaCl, pH before sterilization was 7.0), Furthermore, L-brot
Bacillus sp. No. S3A
It is carried out by adding 0.5 ml of a culture solution containing (FERMP-13573) to 10 ml of L-broth medium and culturing at 30 ° C. for 2 weeks under shaking conditions.
【０００９】培養後、微生物およびニトリルゴムを遠心
分離した後、洗浄して微生物を取り除いたニトリルゴム
の重量変化について測定を行ない、次のような結果を得
た。対比のために記載された親水性基を導入しないニト
リルゴムの場合と比較して、その分解率を１％から５％
に迄上昇させることができた。ニトリルゴム親水性基導入ニトリルゴム
仕込量（ｇ）０．５００．５０
培養後のゴム重量（ｇ）０．４９０．４５
減少率（％）１．００５．００After culturing, the microorganisms and the nitrile rubber were centrifuged, and the weight change of the nitrile rubber from which the microorganisms were removed by washing was measured, and the following results were obtained. The decomposition rate is 1% to 5% compared with the case of the nitrile rubber in which the hydrophilic group is not introduced, which is described for comparison.
I was able to raise it to. Nitrile rubber Hydrophilic group-introduced nitrile rubber Charge amount (g) 0.500.50 Rubber weight after culture (g) 0.490.45 Reduction rate (%) 1.005.00
【００１０】[0010]
【発明の効果】ニトリルゴムを微生物で分解するに際
し、ニトリルゴムに濃硫酸浸漬処理による親水性基を導
入した上で分解処理すると、その分解処理能力が著しく
高められる。When the nitrile rubber is decomposed by microorganisms, if the nitrile rubber is introduced with a hydrophilic group by a dipping treatment with concentrated sulfuric acid and then decomposed, the decomposition treatment ability is remarkably enhanced.
───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.7 識別記号 ＦＩ Ｃ１２Ｒ 1:07） (56)参考文献 特開 平７−258458（ＪＰ，Ａ) 特開 平２−76575（ＪＰ，Ａ) 特開 平２−177883（ＪＰ，Ａ) 米国特許4473641（ＵＳ，Ａ) Ｐｌａｓｔｉｃｓ，Ｒｕｂｂｅｒ ａ ｎｄ Ｃｏｍｐｏｓｉｔｅｓ Ｐｒｏｃ ｅｓｓｉｎｇ ａｎｄ Ａｐｐｌｉｃａ ｔｉｏｎｓ，1992， Ｖｏｌ．17， Ｎ ｏ．１， ｐ．19−21 (58)調査した分野(Int.Cl.7，ＤＢ名) C12N 1/20 C12P 1/04 B09B 3/00 ＢＩＯＳＩＳ／ＷＰＩ（ＤＩＡＬＯＧ) ＪＳＴＰｌｕｓ（ＪＯＩＳ)─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. 7 Identification code FI C12R 1:07) (56) Reference JP-A-7-258458 (JP, A) JP-A-2-76575 (JP, A) JP-A 2-177883 (JP, A) U.S. Pat. No. 4,473,641 (US, A) Plastics, Rubber and Composites Procedures and Applications, 1992, Vol. 17, No. 1, p. 19-21 (58) Fields investigated (Int.Cl. 7 , DB name) C12N 1/20 C12P 1/04 B09B 3/00 BIOSIS / WPI (DIALOG) JSTPlus (JOIS)
Claims (2)
親水性基導入ニトリルゴム分解能を有する微生物を、濃
硫酸浸漬処理による親水性基導入ニトリルゴムを含有す
る培地に添加し、培養することを特徴とするニトリルゴ
ムの分解方法。1. A microorganism belonging to the genus Bacillus and having a ability to decompose a hydrophilic group-introduced nitrile rubber by immersion treatment with concentrated sulfuric acid is concentrated.
A method for decomposing nitrile rubber, which comprises adding to a medium containing a hydrophilic group-introduced nitrile rubber by a sulfuric acid immersion treatment and culturing.
親水性基導入ニトリルゴム分解能を有する微生物を培養
し、得られた培養菌体、培養ロ液またはこれらの処理物
を、濃硫酸浸漬処理による親水性基導入ニトリルゴムと
接触させることを特徴とするニトリルゴムの分解方法。2. A microorganism belonging to the genus Bacillus and having a ability to decompose a hydrophilic group-introduced nitrile rubber by immersion treatment in concentrated sulfuric acid is cultivated, and the obtained cultured microbial cell, culture solution or treated product thereof is A method for decomposing a nitrile rubber, which comprises contacting the hydrophilic group-introduced nitrile rubber with a concentrated sulfuric acid immersion treatment .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11236895A JP3467904B2 (en) | 1995-04-13 | 1995-04-13 | Decomposition method of nitrile rubber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11236895A JP3467904B2 (en) | 1995-04-13 | 1995-04-13 | Decomposition method of nitrile rubber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH08280392A JPH08280392A (en) | 1996-10-29 |
| JP3467904B2 true JP3467904B2 (en) | 2003-11-17 |
Family
ID=14584948
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11236895A Expired - Fee Related JP3467904B2 (en) | 1995-04-13 | 1995-04-13 | Decomposition method of nitrile rubber |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3467904B2 (en) |
-
1995
- 1995-04-13 JP JP11236895A patent/JP3467904B2/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| Ｐｌａｓｔｉｃｓ，Ｒｕｂｂｅｒ ａｎｄ Ｃｏｍｐｏｓｉｔｅｓ Ｐｒｏｃｅｓｓｉｎｇ ａｎｄ Ａｐｐｌｉｃａｔｉｏｎｓ，１９９２， Ｖｏｌ．１７， Ｎｏ．１， ｐ．１９−２１ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08280392A (en) | 1996-10-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5362645A (en) | Heparinase-producing microorganism belonging to the genus bacillus | |
| JP3467904B2 (en) | Decomposition method of nitrile rubber | |
| US4569912A (en) | Production of bilirubin oxidase | |
| JP3178159B2 (en) | Decomposition method of high molecular weight polyethylene glycol | |
| JP2926249B2 (en) | Method for producing alginate lyase | |
| US4275162A (en) | Process for the production of sphingomyelinase | |
| JPS5925596B2 (en) | Oxidative decomposition method of dimethyl phosphate using microorganisms | |
| JP3277680B2 (en) | Microbial degradation of nitrile rubber | |
| JP3055041B2 (en) | α-1,2-mannosidase, method for producing the same, and bacteria producing the same | |
| JPH05211868A (en) | Production of alkali protease | |
| KR900007643B1 (en) | Novel microorganism streptomyces sp.y-125 | |
| JPH0440988B2 (en) | ||
| JP2665533B2 (en) | Agar-degrading enzyme-producing bacterium and method for softening agar medium of plant tissue culture seedling using enzyme produced by the strain | |
| DE2659878B2 (en) | Process for the production of creatinamidinohydrolase | |
| JPS62201571A (en) | Novel chitosanase-producing strain | |
| JPS589674B2 (en) | caprolactam | |
| JPH05236978A (en) | Production of o-methyl-l-tyrosine and l-3-(1-naphthyl) alanine | |
| JPH0574353B2 (en) | ||
| JPS6030B2 (en) | Degradation of acrylamide monomer by bacteria | |
| JPH0685713B2 (en) | Klebsiella oxytoca No. 19-1 and production of alpha cyclodextrin using the same | |
| JPH0616704B2 (en) | Bacterial catalase resistant to fluoride ions and Micrococcus sp. KWI-5 strain | |
| JPS61108393A (en) | Production of pyrogallol | |
| JPH02234672A (en) | Polygalactosamine degradation enzyme e-1 and production thereof | |
| JPS58116681A (en) | Preparation of l-allothreonine aldolase | |
| JPS58146297A (en) | Determination method of polyamine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |