JP3414704B2 - Support system for storage and use of autologous cells - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cell bank system and a method for freezing and thawing cells, and more particularly to personal cells and their cells for the purpose of prevention and diagnosis in order to support personal medical care, beauty, and life planning. The present invention relates to a cell bank system for storing genetic information and a method for freezing and thawing cells.

[0002]

2. Description of the Related Art Heretofore, there has been no place to store cells of a healthy person for the purpose of prevention and diagnosis in case of illness. This was because there was no benefit to storing human cells. However, recently, the usefulness of one's blood has been gradually recognized with the seriousness of the blood shortage, and it has become more common to collect one's blood in advance before the operation and use it during the operation.

Conventionally, the method of preserving cells has been different for each cell, fertilized egg, and tissue (skin sheet).
However, no treatment such as changing the preservation method was taken according to the cell type and its state. In this way, cells, fertilized eggs, etc. have been roughly classified and different preservation methods have been adopted. In this way, the success or failure of cryopreservation of cells can not be understood without actually trying, and the success or failure and the survival rate of the cells before cryopreservation were estimated and considered. The idea of storage method and processing method after defrosting has not been reached.

[0004]

However, in the conventional art, autologous cells (blood, skin, etc.) are not obtained until the patient becomes ill.
Was prepared for surgery and stored for only a short period before surgery. However, if the autologous cells are already affected by the disease at the time of diagnosis of the disease, or if there is such a fact in the past, the autologous cells cannot be used for treatment.
In addition, when a disease is discovered, it may be too late to collect cells in time. Therefore, in preparation for such an emergency situation, it has become necessary to store cells in a young and healthy state as much as possible in advance. In addition, rapid advances in medicine in recent years have enabled cell storage technology, cell proliferation method, and regenerative medicine based on cell differentiation (organization, organ formation). Regenerative medicine is a medicine that tries to create tissues and organs lost due to disease from its own cells without relying on artificial products or transplants from other people or animals. This is a technology that is in the limelight as the mainstream of medical care in the 21st century. However, at present, not all tissues and organs can be regenerated from autologous cells, but it is expected that this medical treatment will be completed in the near future and most cells in the human body will be reproducible. Therefore, it is very significant to cryopreserve the autologous cells in advance and construct a system to be used when necessary.

The object of the present invention has been made in view of the problems of the above-mentioned conventional technique, in which the cells of the client are collected in advance, and information on the cells is obtained in response to the request from the client. The cell bank system and the cell freezing and thawing method that allows the cells to be delivered to tissue culture companies and drug / cosmetic manufacturing companies, where they can be expanded, differentiated, or made-to-order drugs and cosmetics are provided. The purpose is to do.

[0006]

In order to achieve the above object, a human cell bank (hereinafter abbreviated as HCB) of the present invention receives a request for browsing data on cells stored therein, and receives by the receiving means. And a display unit for displaying data regarding the stored cells. By realizing the cell bank system including each of the above means, it becomes possible for the client to browse the information on cells at any time.

[0007] In another aspect, receiving means for receiving a request for viewing data on stored cells, personal identification means for requesting personal identification in response to the reception by the receiving means, and personal identification by the personal identification means And display means for displaying data on the stored cells,
It is a thing. By realizing the cell bank system including each of the above means, it is possible to prevent unauthorized access to the cell bank system, and thereby to prevent leakage of the personal information of the client and information regarding cells and their genetic information. In another aspect, the data regarding the cells and the genetic information thereof include the number of cells, effective storage period, or storage method.

By adopting such a data structure, the client can browse the number of cells, effective cell storage period, and storage method at any time. In another aspect, the cells are frozen, the frozen state is maintained for a first predetermined time, the cells are thawed, and the cells are cultured for a second predetermined time, and then the ratio of viable cells is calculated. The method determines the preservation method or preservation period of the cells based on the result. By adopting such a cell freezing method or thawing method, it becomes possible to determine in advance whether cells can be stored and the effective storage period, and as a result, waste can be eliminated.

[0009] In still another aspect, the method or period for preserving the cells is different depending on the cell type and cell state. By taking such storage or storage period of cells, the optimal storage method and storage period can be selected according to the type and state of individual cells.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT A preferred embodiment of the present invention will be described in detail below with reference to the accompanying drawings. The present invention collects cells of each client in advance,
The cells are stored according to the purpose, and when the client himself needs it, he / she confirms his / her cell storage status on the Internet and gives the necessary instructions to the HCB storage manager. The storage manager performs the treatment according to the purpose according to the request of the client and delivers the client's cells to the designated time and place. Further, according to the requester's instructions, the stored cells are subjected to genetic information and drug sensitivity test using the cells, and the result is notified to the requester.
The analysis and test status will be disclosed to the client himself / herself on the Internet at any time, and in-home consent and information disclosure will be thoroughly implemented according to the question and answer with the client. In addition, the purpose is to have a counselor specializing in cell medicine and regenerative medicine resident and provide counseling while explaining the medical technology and science considered at this stage.

FIG. 1 shows an H according to an embodiment of the present invention.
It is a block diagram which shows the whole structure of CB. As shown in FIG. 1, the cell bank system according to this embodiment, HC
B1 and a tissue culture company 2 that is connected to the Internet 6 and outsourced from the HCB1 to grow and differentiate the cells of the client 5, and a drug / cosmetic manufacturing company that manufactures drugs and cosmetics based on cell extracts. 3 and a hospital 4 for surgery and treatment using cells that have been grown and differentiated in a tissue culture company 2.
And a client 5 who wishes to freeze-store the cells in HCB1
It consists of and. In the present embodiment, the tissue culture company 2, the drug / cosmetics manufacturing company 3, and the hospital 4 are conveniently operated.
It is assumed that all computer terminals are connected to the Internet 6.

In FIG. 1, the HCB 1 is stored in the cell storage facility 13 which is a cell storage means for cryopreserving the cells collected from the client 5, and the personal information of the client 5 and the cell storage facility 13. A memory 11 that is a storage unit that stores information about cells, a host computer 12 that controls the entire HCB 1, and a server 14 that connects to the Internet 6 and enables the client 5 to browse the HCB 1 home page. ,,.

In the above, information on cells includes HIV-1 (human immunodeficiency virus-1: AIDS virus), HBV (hepatitis B virus: hepatitis B virus), HCV (hepatitis C virus: hepatitis C virus). ), Cell viability, cell morphology, cell proliferation, microbial contamination (mycoplasma, bacteria, yeast, mold, etc.) and other test results. Further, in this example, a cryopreservation method is selected according to the state of the cell, and a method of preparing a countermeasure after thawing in advance is adopted, but the state of the cell includes the following items. .

1) Total number of cells (when the number of cells is small, the number of cells after freeze-thawing also decreases accordingly, and it becomes difficult to maintain the survival of the cells). 2) Resistance to cryopreservation (resistance differs depending on cell type). 3) Elapsed time after separation from the human body (if the time after separation from the human body is too long, the vitality of cells decreases). 4) Preservation method until cryopreservation (preservation method taken after separating cells such as whether cells were placed under culture conditions, immersed in medium, or chilled, etc. The less the damage to the cells, the better the recovery rate after cryopreservation).

The purpose of storing cells in the cell storage facility 13 in the present embodiment is roughly classified into the following seven. (1) From cells, for example, paternity testing and SNP (Single N
ucleotide Polymorphism: A storage for the purpose of obtaining genetic information (primary information) including genetic diagnosis such as single nucleotide polymorphism. (2) Storage for the purpose of obtaining data of drug sensitization test for anticancer drug, cosmetics, etc. and gene of tested cells by culturing and proliferating cells. (3) Storage for the purpose of culturing and proliferating cells, and extracting, separating, and purifying components such as a target protein from the cells.

(4) Storage of thawed cells as they are, after proliferation and differentiation, or cells, tissues, or organs that have been frozen after tissue formation or organ formation before use for transplantation medicine. (5) Storage for proliferating cells after thawing, or tissue and organ formation after proliferation and differentiation for use in transplantation medicine. (6) Storage of ES (Embryonic Stem Cell) cells having totipotency (having the ability to differentiate into various tissues and organs). (7) Outsourced storage from the hospital.

For cell storage for the purposes of (1) and (2), cells of 5 individual clients are collected and stored in HCB1.
When storing cells for the purposes of (3) and (4), the cells should be stored by the client 5.
It is taken from and is transported to HCB1 for storage. According to instructions from the client, the cells are made to sleep, proliferate and differentiate, or the cells are transported to the hospital 4 via the tissue culture company 2,
Returned to the requester 5. For the purpose of storing cells for the purposes of (5) and (6), the cells should be proliferated and differentiated before storage, or those that have been proliferated or differentiated (organization, organ formation) by the tissue culture company 2. Return to cell bank and store. Information obtained by analysis of genes and sensitization tests can be sent only to the five requesters and via the Internet 6 to the requester 5, HCB1.
May be shared among participants of the cell bank system such as Hospital 4. In this case, obtaining the consent of the client is a prerequisite. At this time, the doctor and the medical / beauty coordinator are the only third parties who can collate the requester name with the data.

As a method for collecting cells, (1) a method using a scalpel, a laser (surgical operation), etc. according to the intention of the client, (2) umbilical cord blood at birth, wisdom tooth (third molar) A method of collecting at a time when it can be extracted, such as tooth extraction,
(3) Also, there are three conceivable methods, such as accidents such as illness and accident.

Freezing / thawing of cells in the present embodiment is performed as follows. First, pre-cryopreservation is performed to determine whether or not cells can withstand cryopreservation and to predict a treatment method for thawing. There are two methods for this pre-experiment.
(1) A cell preservation tube is packed with a heat insulating material such as a paper towel, placed in a freezer at −80 ° C. for 1 hour, immersed in liquid nitrogen for 10 minutes or more to be frozen, and then thawed by a usual method. Alternatively, (2) flash freezing (freezing in liquid nitrogen for 10 minutes or more and then thawing by a normal method. Next, the number of thawed cells is measured.
The street method is adopted. In the case of floating cells, the cells are cultured for 1 hour, stained with a trypan blue solution or the like, and the number of living cells and dead cells are counted with a hemocytometer to calculate the cell survival rate.
In the case of adherent cells, after thawing, the cells are cultured for 1 hour and the survival rate is calculated from the number of adhered cells. In addition to the Trypan Blu-solution, an MTT reagent for measuring cell viability may be used.

This MTT reagent is available from Gay, et al, The Livi
ng Skin Equivalent as a Model InVitro for Ranking
the Toxic Potential of Dermal Irritants, Toxic. In
Vitro, 6, 303-315 (1992). The metabolic reduction of soluble tetrazolium salts to blue formazan precipitates depends on the presence of viable cells with intact mitochondrial function and is used to calculate cytotoxicity. The MTT reagent is, for example, from Toyobo
It is marketed under the trade name E.

The above description is illustrated below. Collected cells ↓ Partially freeze or flash a portion of cells in a short time ↓ Freeze for 10 minutes ↓ Thaw ↓ Culture for 1 hour ↓ Calculate the ratio of live / dead cells (or survival rate) ↓ Preservation of cells Judge the possibility ↓ Take action according to the result

FIG. 2 is a table showing a comparison of the cell numbers of the primary osteoblasts and the primary human epidermal keratinocytes by the pre-cryopreservation method and the general cryopreservation method. Hereinafter, in order to show the characteristics of the pre-cryopreservation method, a comparison will be made between the pre-cryopreservation method and the general (simple) cryopreservation method using primary human osteoblasts and primary human epidermal keratinized tissue.

First, primary human osteoblasts and primary human epidermal keratinocytes were prepared at 1 × 10 ⁶ cells / ml, respectively.
Medium containing% DMSO, 20% FBS (fetal bovine serum) and commercially available cell banker (BLC-1: Juzen Kagaku Kogyo)
, Put it in a cryotube, then
(A) In the pre-cryopreservation method, the cryotube containing the cells is wrapped with a paper towel 5 to 6 times, and then it is -8 as it is.
Place in freezer at 0 ° C for 1 hour and then soak in liquid nitrogen for 10 minutes. (B) In a general cryopreservation method, a cryotube is put in a Bicell (Japan Freezer) which has been cooled to 4 ° C. in advance, and put in a freezer at −80 ° C. as it is. After 2 hours, the tube is taken out from the bicell and immersed in liquid nitrogen for 10 minutes. Then (A), (B)
With both methods, remove the tube from liquid nitrogen and immediately
The cells are thawed in a 7 ° C. water bath, and the cells are placed in a culture medium containing 20% FBS that has been cooled to 4 ° C. in advance, and the cryopreservation solution is removed by centrifugation. Finally, the cells are suspended in an optimal medium stored at 37 ° C., cultured for 1 hour, and then the adhered cells are counted.

The survival rate and viability of cells, which serve as a preservation criterion, differ depending on the cells. For example, if the survival rate and viability are 10% or less, the standard preservation period is 5 years. And the cell culture after thawing has a small area to increase the cell density,
For example, it is carried out in a Petri dish of 3.5 cm, and as soon as the cells adhere, the medium is exchanged to quickly remove a large amount of dead cells. It is to be noted that FIG. 2 shows a comparison of cell viability depending on the type of cells and the difference in freezing conditions between the pre-cryopreservation method and the general cryopreservation method which is performed as a standard method.

As a result, 10% DMSO as a cell cryopreservation solution was added to the primary human osteoblasts and the primary human epidermal keratinocytes.
From a study using a medium containing 0% FBS and a commercially available cell banker, there was no difference between the general cryopreservation method without using the program freezer and the pre-cryopreservation method.
Therefore, it is considered that the evaluation by the pre-freezing preservation method is the same as the evaluation by the general cryopreservation method. As a guide, if the cell viability and viability are 10% or less, the freezing method using a program freezer is performed, and the preservation rate is assumed to be about 5 years. If there are no viable cells or if the survival rate is 0.1% or less, cryopreservation is not performed and a cell cryopreservation solution suitable for this cell is examined.

FIG. 3 is a diagram showing a functional block diagram of software executed in the host computer of the HCB. As shown in FIG. 3, the software of the HCB 1 includes a window / reception 16 unit that accepts a requester's membership, an identity confirmation unit 17 that is an identity verification unit that confirms whether the client is actually the client, and a client Questionnaire unit 18 to confirm the request contents and intention to store cells
A determination unit 19 for determining whether or not to accept the membership to the HCB 1, an information management unit 20 for storing the information of the client who has joined, and a cell storage unit for monitoring the cells stored in the cell storage facility 13. 21 and 21.

In FIG. 3, each block shows each software unit from the acceptance of the client to the storage of the cells. Ask the client to answer the questionnaire after the requester confirms the person's identity according to the contents of the request and selection from the home computer on the Internet or at the business office or reception terminal. This is a procedure to determine the meaning of confirming the request of the person himself and the strength of his will. The determination unit 19 analyzes the result of the questionnaire and determines whether to refuse the membership to the cell bank or accept the membership. If you decline the membership, inform the client accordingly. When accepting the membership, the information management unit 20 saves it as the information of the requester, and also notifies the cell storage facility 13 by the cell storage unit 21 if necessary. If you decide to accept the membership, the information management unit 20 will give you a permission ID.
The number will be issued to the client. The client uses this ID number for the next instruction and request.

FIG. 4 is a diagram showing the contents of the counter and the reception by work. It consists of membership 30 to HCB, cell storage registration 40, cell collection reservation 50, cell collection 60, and cell inspection 70. The case of each business will be described below.

FIG. 5 is a flow chart showing the procedure of membership work. The client performs the enrollment procedure from the HCB homepage on the Internet with the personal computer at home or from the terminal installed at the HCB business office (step S3
1, S32). Answer the questionnaire (step S3
3) The result is judged (step S34), and if it is judged that the membership is valid from the result of the judgment, it is confirmed whether pre-registration is required (step S36). Make a reservation and issue a temporary ID number to the requester. If the membership is refused in step S34,
The reason is explained to the client (step S35).

FIG. 6 is a flow chart showing a procedure when a client to whom a temporary ID number is issued performs cell storage registration. This registration is done by having the client come to the business site and the client and the person in charge of the window agree upon it. The person in charge of the window asks the requester to input the temporary ID and confirms whether or not the person is the original person (step S41). In addition to passwords, voiceprints, fingerprints, irises, etc. can be assumed for the subsequent identification method. Next, it is confirmed whether or not to register (step S42), and if registered, a questionnaire is answered (step S43). Next, the result of the questionnaire is judged (step S44), and if the cell storage registration is permitted based on the judgment result, the contract is made with the client, the contents of the questionnaire are saved, the cell collection reservation system is contacted, and the request is made at the same time. Issue ID ₁ to the person (step S46). If the cell storage registration is not permitted in step S44, the reason is explained to the client (step S45).

FIG. 7 is a flow chart showing the procedure for cell collection reservation. The client enters the cell collection reservation system on the home page by entering ID ₁ using his or her personal computer or a terminal installed in the business office, confirms the person himself (step S51), and then answers the questionnaire (step S5).
3) The result is judged (step S54), and if the reservation is possible, the reservation item is input (step S56) and the confirmation is confirmed (step S52). And the ID ₂ is issued to the requester. When the result of the questionnaire is determined to be improper in step S54, the reason is explained to the requester (step S55).

FIG. 8 is a flow chart showing the procedure of cell collection. The client uses the terminal installed at the business site to ID
Enter ₂ and enter the cell collection facility reception system on the home page to confirm your identity (step S61),
It is confirmed whether cells should be collected (step S62). If cells should be collected, the questionnaire is answered (step S6).
3) The result is determined (step S64), the result is managed as personal information, and permission for cell collection is issued. With the permission of cell collection, the cell collection certificate and ID ₃ are issued to the client (step S66). And with permission to collect cells,
Have the client submit a cell consent form and collect the cells. Transport the collected cells to the cell storage facility. In step S64, if the determination result is not good, the reason is explained to the client (step S65).

FIG. 9 is a flow chart showing the procedure for requesting a cell test. The client enters the cell test request screen by entering ID ₃ or ID ₄ on the HCB home page from his or her personal computer, and proceeds as before until now (step S
71). ID ₄ is an ID number issued to a client who has already performed a cell test. Confirm whether to inspect (step S72), answer the questionnaire (step S73), determine whether or not the cell inspection is possible from the result of combining the questionnaire with the inspection item and purpose (step S74), and if necessary before the inspection Counseling, confirming inspection items (steps S76 and S77), and then proceeding to inspection and analysis. Also, these results and data are stored as personal information. If it is determined in step S74 that the result of the inspection is impossible, the reason is explained to the client (step S75).

FIG. 10 is a flow chart showing the procedure in the case of an item that does not require counseling. The client can use the ID ₃ or ID from his or her personal computer to the HCB website.
After inputting ₄ , the person can be confirmed (step S8
0), the inspection data about the person and the explanation thereof are displayed on the screen which is the display means (step S81). The inspection data and its description can be viewed according to the screen display (steps S82 and S83). When the questionnaire is answered (step S84), the judgment unit 19 judges from the result whether or not counseling is necessary (step S85). If it is judged that counseling is necessary, the counselor is reserved and introduced (step S86). If it is determined in step S85 that counseling is unnecessary, it is confirmed whether advice is desired (step S87),
If an advisor is required, the advisor or specialist is introduced and an interview is conducted with them (step S88). When a member makes a cell storage registration, he / she will come to the business office and have an interview with an advisor. Therefore, in order to save waiting time, we ask that you make a preliminary registration in the sense of making a reservation. As a result, registration is reserved and the screen is closed.

FIG. 11 is a flow chart showing the procedure in the case of an item requiring counseling. The client sends ID ₃ or ID ₄ to the HCB homepage from the terminal of the office.
Is input (step S90), a registration screen is displayed (step S91). After the person is confirmed, it is determined whether or not the counseling is received (step S9).
2). If you have not received the counseling of the test result you want to know, that is, if it is the first time to know, the result (data) will be outlined (step S96), and if you want to know more in detail (step S97), the questionnaire If the result also supports it (step S98), the counselor makes an appointment and introduces the result (step S99). On the other hand, when the counseling has already been received from the counselor and the result has been heard, the result (data) can be viewed (step S93). If there is a consultation about the result (step S94), an expert is introduced (step S95).

FIG. 12 is a display example from the HCB home page screen to the personal page screen. When the member whose ID number has been issued from the HCB homepage selects "member's page", the next screen is displayed, and "registration reservation" or "cell collection reservation" is selected from this screen. here,
When "Personal page" is selected, the next screen is displayed. From this screen, the user's data is confirmed and the inspection request is made. On the first screen, only the requester whose ID has been issued can proceed to the screen of the next member.

FIG. 13 is a selection screen for option data. When "Option data" is selected on the screen of the personal page shown in FIG. 11, this screen is displayed.
Select "Analysis data", "Data dispatch", etc. from this screen. FIG. 14 is a display screen of personal data. Figure 11
When "Personal data" is selected on the screen of the personal page shown in, this screen is displayed. The requester's ID number, basic data, etc. are displayed on this screen. FIG. 15 shows an inspection request selection screen. This screen is displayed when "inspection request" is selected on the screen of the personal page shown in FIG. On this screen, selecting "item" displays a screen for selecting the contents of the item, and selecting "purpose" displays the target selection screen.

FIG. 16 shows a counseling selection screen. When "Counseling" is selected on the screen of the personal page shown in FIG. 11, this screen is displayed. Select "Consultation" or "Questionnaire" from this screen. FIG. 17 shows a work status selection screen. When "work status" is selected on the screen of the personal page shown in FIG. 11, this screen is displayed. From this screen, select "Storage location of cells" or "Progress".

The recording medium storing a program for executing the operation of the cell bank system may be a ROM, or a program reading device (not shown) such as a CD-ROM driver is provided as an external storage device. It may be a CD-ROM or the like that can be read by being inserted therein. Further, the recording medium may be a magnetic tape, a cassette tape, a floppy (registered trademark) disk, a hard disk, MO / MD / DVD, etc., or a semiconductor memory.

[0040] It should be noted that the present invention, the Internet in tissue culture companies, chemicals / cosmetics manufacturing company, has been described taking the case of a hospital or the like is connected to the example, without necessarily Ru limited to this form, for example, the gym It may be connected to a restaurant or the like so that it can be used for planning a weight loss schedule for dieting and for creating a menu.
Further, since the cell bank system 1 uses the Internet, it goes without saying that it is possible to provide services not only in Japan but also when a client travels or makes a business trip abroad.

[0041]

As described above, according to the present invention, the following remarkable effects are obtained. (1) By analyzing cells stored in the past, accurate disease diagnosis and paternity test can be performed. (2) By preparing autologous cells in advance for disease, autologous organs can be created from autologous cells, it is not necessary to find a new donor for transplantation, and the survival rate is significantly improved. (3) You will be able to independently consider your own health management and treatment. (4) If an organ made of autologous cells is transplanted, it is not necessary to use an immunosuppressive drug, so that the pain can be reduced as the side effects are reduced. (5) The spread of organ trafficking can be prevented. (6) Since medical agents can be prepared according to one's constitution and type of illness, medical costs can be kept low. (7) Since cosmetics suitable for one's own skin can be selected and manufactured, troubles such as rough skin and inflammation can be prevented.

[Brief description of drawings]

FIG. 1 is a block diagram showing an overall configuration of an HCB according to an embodiment of the present invention.

FIG. 2 is a table showing a comparison of cell numbers of primary osteoblasts and primary human epidermal keratinocytes by a pre-freezing preservation method and a general cryopreservation method.

FIG. 3 is a diagram showing a functional block diagram of software executed in an HCB host computer.

FIG. 4 is a diagram showing the contents of a counter and a reception by work.

FIG. 5 is a flowchart showing a procedure of membership work.

FIG. 6 is a flowchart showing a procedure in the case where a client who has been issued a temporary ID number performs cell storage registration.

FIG. 7 is a flow chart showing the procedure of cell collection reservation.

FIG. 8 is a flowchart showing a procedure of cell collection.

FIG. 9 is a flowchart showing a procedure for requesting a cell test.

FIG. 10 is a flowchart showing a procedure in the case of an item that does not require counseling.

FIG. 11 is a flowchart showing a procedure for an item that requires counseling.

FIG. 12 is a display example from the HCB home page screen to the personal page screen.

FIG. 13 is a selection screen for option data.

FIG. 14 is a display screen of personal data.

FIG. 15 is a screen for selecting an inspection request.

FIG. 16 is a counseling selection screen.

FIG. 17 is a work status selection screen.

[Explanation of symbols]

1 HCB (Human Cell Bank) 2 tissue culture companies 3 Pharmaceutical / cosmetic manufacturing companies 4 hospitals 5 users 6 Internet 11 memory 12 Host computer 13 Cell storage 14 servers 16 counter / reception unit 17 Identity verification unit 18 Questionnaire Unit 19 Judgment unit 20 Information management unit 21 Cell storage unit 30 enrollment 40 Registration 50 cell collection reservation 60 cell collection 70 Cell test

Claims (3)

(57) [Claims] [Claim 1] Autologous cells collected from a client are used according to the purpose.
Stored in a cell storage facility for freezing storage
A memory for storing data on the autologous cells
And a host computer that controls the memory,
A server connected to the host computer and the server
And computer terminal via the Internet
A system for utilizing network, the host computer, automatic judgment stearate based on the questionnaire input data of a predetermined item
To determine whether or not the client can join the membership
And the automatic judgment step based on the questionnaire input data of the predetermined items.
To determine whether the client's cell storage registration permission is granted.
Means and automatic judgment step based on questionnaire input data of predetermined items.
To determine whether to permit cell collection from the client.
And automatic judgment steps based on questionnaire input of predetermined items.
A means for executing and determining whether or not to carry out a cell test.
At least, the server is the server stored by the client via the Internet.
Receiving means for receiving the browsing request information of the own cell data, and a book for requesting personal identification in response to the receiving means.
Based on the person confirmation means and the result of the person confirmation performed by the person confirmation means.
And the requester of the data via the Internet.
From the client regarding the means of sending to the computer terminal of
Receiving means for receiving the request information and sending the inspection result information to the computer terminal of the client.
And the treatment of the stored cells according to their purpose.
Receive the instruction information from the requester and entrust the instruction information
Means for transmitting to a computer terminal of a cell culture company, and multiplication by the treatment at the contracted cell culture company,
Differentiated cells or extracts of the cells for a specified time
The instructions information from the requester about to be transported to the location and
Means for receiving, at least the above-mentioned expanded, differentiated cells or extracts of the cells,
Be used only for medical treatment or beauty of the client
Assistance for storage and utilization of autologous cells characterized by
Tem. 2. The data includes genetic information and cells
The number, effective storage period and storage method are included.
A support for storage and use of autologous cells according to claim 1.
Assistance system. 3. Proliferated and differentiated cells or extraction of the cells
The place where goods are transported is a pre-registered hospital, makeup
Characterized by being either a product manufacturer or a drug manufacturer
Storage and use of the autologous cell according to claim 1 or 2.
Support system for.