JP3336659B2 - Mass spectrometer - Google Patents
Mass spectrometerInfo
- Publication number
- JP3336659B2 JP3336659B2 JP03963993A JP3963993A JP3336659B2 JP 3336659 B2 JP3336659 B2 JP 3336659B2 JP 03963993 A JP03963993 A JP 03963993A JP 3963993 A JP3963993 A JP 3963993A JP 3336659 B2 JP3336659 B2 JP 3336659B2
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- capillary
- mass spectrometer
- potential
- spraying
- current
- Prior art date
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- Electron Tubes For Measurement (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、生体物質の分析に重要
なキャピラリ電気泳動と質量分析計とを結合した装置、
すなわち、キャピラリ電気泳動・質量分析計におけるイ
ンタフェースに関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an apparatus combining capillary electrophoresis and a mass spectrometer, which are important for the analysis of biological substances.
That is, it relates to an interface in a capillary electrophoresis / mass spectrometer.
【０００２】[0002]
【従来の技術】生体物質の分析の分野では、分離能力に
優れた方法と分析能力の優れた方法との結合が重要視さ
れている。分離に関しては、最近、極微量の試料を扱う
ことができ、かつ、分離能力に優れたキャピラリ電気泳
動法が注目されている。この手法は、内径０.１ mm以下
のキャピラリ（毛細管）に試料を導入し、キャピラリの
両端に高電圧を印加して電気泳動させ、各々の試料の易
動度の差により分離する方法である。2. Description of the Related Art In the field of analysis of biological materials, importance has been attached to the combination of a method having excellent separation ability and a method having excellent analysis ability. Regarding separation, recently, a capillary electrophoresis method capable of handling a very small amount of sample and having excellent separation ability has attracted attention. In this method, a sample is introduced into a capillary (capillary tube) having an inner diameter of 0.1 mm or less, a high voltage is applied to both ends of the capillary, electrophoresis is performed, and separation is performed based on a difference in mobility of each sample. .
【０００３】通常の電気泳動法ではジュール発熱が問題
となって分離性能を損ねるため、あまり高い電圧を印加
することはできない。一方、キャピラリ電気泳動法の場
合、内径が小さいためインピーダンスが大きく、高電圧
が印加可能で、高速で分離できる特徴を持つ。また、キ
ャピラリ電気泳動法では、一度の分析でキャピラリ中に
導入される試料溶液の体積は数ナノリットルにすぎず、
微量な生体試料を扱う場合に特に有利となる。キャピラ
リ電気泳動法の検出器として、試料の分子量という定性
情報が得られる質量分析計が注目されており、キャピラ
リ電気泳動と質量分析計とを結合したキャピラリ電気泳
動・質量分析計の発展が望まれている。[0003] In a usual electrophoresis method, Joule heat is a problem and the separation performance is impaired, so that a very high voltage cannot be applied. On the other hand, the capillary electrophoresis method has a feature that the impedance is large because the inside diameter is small, a high voltage can be applied, and separation can be performed at high speed. Also, in capillary electrophoresis, the volume of a sample solution introduced into a capillary in one analysis is only a few nanoliters,
This is particularly advantageous when handling a very small amount of biological sample. As a detector for capillary electrophoresis, a mass spectrometer that can obtain qualitative information on the molecular weight of a sample is attracting attention, and the development of a capillary electrophoresis / mass spectrometer that combines capillary electrophoresis and a mass spectrometer is desired. ing.
【０００４】図８は従来のキャピラリ電気泳動・質量分
析計の全体の構成図を示すブロック図である。バッファ
槽１にバッファ溶液の充填されたキャピラリ２の一端を
挿入し、キャピラリ２の他端との間に高圧電源３により
高電圧を印加し、キャピラリ２中に導入された試料を電
気泳動させる。キャピラリ２の末端に到達した試料はイ
オン源４に導入され、気体状のイオンへと変換される。
生成されたイオンは質量分析部５へと導入される。質量
分析部５は排気系６により真空に排気されている。質量
分析されたイオンは検出部７で検出され、信号は信号ラ
イン８ａを介してデータ処理装置９へと送られ処理され
る。FIG. 8 is a block diagram showing the overall configuration of a conventional capillary electrophoresis / mass spectrometer. One end of the capillary 2 filled with the buffer solution is inserted into the buffer tank 1, a high voltage is applied between the capillary 2 and the other end of the capillary 2 by the high-voltage power supply 3, and the sample introduced into the capillary 2 is electrophoresed. The sample that has reached the end of the capillary 2 is introduced into the ion source 4 and converted into gaseous ions.
The generated ions are introduced into the mass spectrometer 5. The mass analyzer 5 is evacuated to a vacuum by an exhaust system 6. The mass-analyzed ions are detected by the detection unit 7, and the signal is sent to the data processing device 9 via the signal line 8a for processing.
【０００５】このように、キャピラリ電気泳動・質量分
析計の構成は簡単であるが、キャピラリ電気泳動が一般
に溶液中の試料を扱うのに対し、質量分析計が真空中の
イオンを扱うという相性の悪さから、キャピラリ電気泳
動・質量分析計の開発では、キャピラリと質量分析計と
を結ぶイオン源の部分が最も重要である。As described above, the configuration of a capillary electrophoresis / mass spectrometer is simple. However, the capillary electrophoresis generally handles samples in a solution, whereas the mass spectrometer handles ions in a vacuum. Due to the badness, the part of the ion source connecting the capillary and the mass spectrometer is the most important in the development of the capillary electrophoresis / mass spectrometer.
【０００６】ところで、イオン源として重要視されてい
るのは、試料を含む溶液を噴霧し、溶液中に含まれる試
料をイオン化して質量分析部へと取り込む噴霧イオン化
法を用いたイオン源である。噴霧イオン化法の例とし
て、アナリティカル ケミストリ １９８８年，６０
巻，１９４８頁(Analytical Chemistry, 60 (1988) 194
8）に記載されている静電噴霧法について説明する。[0006] By the way, what is regarded as important as an ion source is an ion source using a spray ionization method in which a solution containing a sample is sprayed, and the sample contained in the solution is ionized and taken into a mass spectrometer. . As an example of the spray ionization method, Analytical Chemistry 1988, 60
Volume, 1948 (Analytical Chemistry, 60 (1988) 194
The electrostatic spraying method described in 8) will be described.
【０００７】図９は静電噴霧イオン源を備えたキャピラ
リ電気泳動・質量分析計の構造を示す断面図である。フ
ューズドシリカ製キャピラリ２はステンレス管１０の中
に挿入されている。キャピラリ２とステンレス管１０と
の間には一定流量のシースフローが流される。電気浸透
流により送り出される試料溶液はキャピラリ２の末端で
シースフローと混合される。ステンレス管１０と対向電
極１１との間に数キロボルトの電圧を印加すると、溶液
が噴霧される、いわゆる静電噴霧現象が起きる。静電噴
霧により生成した液滴に対し、気化用ガス噴出口１２か
ら窒素などのガスを吹きかけ、液滴の気化を促進させ
る。このようにして生成されたイオンはイオン導入細孔
１３ａ，１３ｂから真空中に取り込まれ、質量分析され
る。FIG. 9 is a sectional view showing the structure of a capillary electrophoresis / mass spectrometer provided with an electrostatic spray ion source. The fused silica capillary 2 is inserted into a stainless steel tube 10. A sheath flow having a constant flow rate flows between the capillary 2 and the stainless steel tube 10. The sample solution delivered by the electroosmotic flow is mixed with the sheath flow at the end of the capillary 2. When a voltage of several kilovolts is applied between the stainless steel tube 10 and the counter electrode 11, a so-called electrostatic spray phenomenon occurs in which the solution is sprayed. A gas such as nitrogen is blown from the vaporizing gas outlet 12 to the droplet generated by the electrostatic spraying to promote the vaporization of the droplet. The ions thus generated are taken into the vacuum from the ion introduction pores 13a and 13b and subjected to mass analysis.
【０００８】[0008]
【発明が解決しようとする課題】図１０に、図９に示し
た構造の電気回路の構成図を示す。電気泳動用には直流
数十キロボルトの電源１４，静電噴霧用には直流数キロ
ボルトの電源１５が必要となる。キャピラリ２の両端に
数十キロボルトの電位が印加されると、キャピラリ２中
に電流Ｉ0 が流れる。電流Ｉ0 の一部は噴霧された液滴
によって電流Ｉ1として対向電極１１へと流れる。一般
に、キャピラリ２中を流れる電流Ｉ0 は約１０マイクロ
アンペアであるのに対し、静電噴霧により対向電極１１
側へ流れる電流Ｉ1は１マイクロアンペア以下にすぎな
い。従って、過剰な電流はＩ2として静電噴霧用電源１
５へと流れる。この陽極側から流れ込む電流Ｉ2 によっ
て、静電噴霧用電源１５が安定に動作しないという問題
があった。FIG. 10 shows a configuration diagram of an electric circuit having the structure shown in FIG. For the electrophoresis, a power supply 14 of several tens of volts DC is required, and for the electrostatic spraying, a power supply 15 of several volts DC is required. When a potential of several tens of kilovolts is applied to both ends of the capillary 2, a current I 0 flows through the capillary 2. Some of the current I 0 flows to the opposite electrode 11 as a current I 1 by spray droplets. Generally, the current I 0 flowing through the capillary 2 is about 10 μA, while the counter electrode 11
Current I 1 that flows to the side is only less than 1 microampere. Therefore, the excess current is regarded as I 2 as the power source 1 for electrostatic spraying.
Flows to 5. The current I 2 flows from the anode side, electrostatic spraying power source 15 has a problem that does not operate stably.
【０００９】例えば、電流Ｉ2 が流れ込むと静電噴霧用
電源１５によりステンレス管１０の電位を制御すること
ができなくなり、ステンレス管１０の電位は電流Ｉ2 と
静電噴霧用電源１５の内部抵抗で決まる電位になった。
ステンレス管１０に印加される電圧が制御できなくなる
と、静電噴霧が不安定になり、従ってイオンも安定して
観測できなくなった。For example, when the current I 2 flows, the potential of the stainless steel tube 10 cannot be controlled by the electrostatic spray power source 15, and the potential of the stainless steel tube 10 is controlled by the current I 2 and the internal resistance of the electrostatic spray power source 15. The potential was determined by
When the voltage applied to the stainless steel tube 10 could not be controlled, the electrostatic spray became unstable, so that ions could not be observed stably.
【００１０】この様な場合、図１１に示すように、電気
泳動用高圧電源１４含む回路を電源１５により浮かせる
構成が用いられるが、この構成では絶縁トランス等が必
要になる上、十分な安全上の対策が必要となり、簡便で
はない。キャピラリ電気泳動法では、キャピラリの温度
制御を行えば、より高い電圧を使用することにより、よ
り高速で分離が可能となる。また、高い分離能力を実現
するには、バッファ溶液の組成を幅広く変え、最適な組
成を見つけ出すことが重要になる。このため、バッファ
溶液の組成によっては、バッファ溶液の電気伝導度が高
くなり、キャピラリ中に大きな電流が流れることがあ
る。従って、キャピラリ中を流れる電流値によらず安定
に噴霧できる静電噴霧イオン源の開発が望まれていた。In such a case, as shown in FIG. 11, a configuration is used in which a circuit including a high-voltage power supply 14 for electrophoresis is floated by a power supply 15, but this configuration requires an insulating transformer and the like, and provides sufficient safety. It is not easy to use. In the capillary electrophoresis method, if the temperature of the capillary is controlled, the separation can be performed at a higher speed by using a higher voltage. Further, in order to realize a high separation ability, it is important to change the composition of the buffer solution widely and find an optimum composition. For this reason, depending on the composition of the buffer solution, the electrical conductivity of the buffer solution increases, and a large current may flow through the capillary. Therefore, there has been a demand for the development of an electrostatic spray ion source capable of spraying stably irrespective of the current value flowing through the capillary.
【００１１】本発明の目的は、キャピラリ中を流れる電
流値によらず、安定に噴霧できる静電噴霧イオン源を提
供することにあり、キャピラリ電気泳動と質量分析計と
を直結したキャピラリ電気泳動・質量分析計において、
安定に噴霧可能な静電噴霧イオン源を備え、イオンを安
定して観測できるキャピラリ電気泳動・質量分析計を提
供する。An object of the present invention is to provide an electrostatic atomization ion source capable of stably spraying irrespective of the value of current flowing in a capillary, and to provide a capillary electrophoresis system in which capillary electrophoresis and a mass spectrometer are directly connected. In a mass spectrometer,
Provided is a capillary electrophoresis / mass spectrometer including an electrostatic spray ion source capable of stably spraying and capable of stably observing ions.
【００１２】[0012]
【課題を解決するための手段】上記目的、すなわち静電
噴霧イオン源の安定動作を可能とするために、混合物を
分離するためのキャピラリ、このキャピラリから送られ
てくる試料溶液を静電噴霧させイオンを生成する静電噴
霧イオン源、この生成したイオンを真空部に導入するた
めのイオン導入細孔、及びこの導入されたイオンを質量
分析するための質量分析部とを備えた質量分析計におい
て、噴霧細管にインピーダンスを有する負荷を接続し、
負荷に流れる電流による電位降下により、静電噴霧を行
うための噴霧細管の電位を数キロボルトに保持する。よ
り詳細には、負荷を流れる電流値に応じて負荷のインピ
ーダンスを変えることにより、噴霧細管の電位を一定に
保つ。In order to achieve the above object, that is, to enable stable operation of an electrostatic spray ion source, a capillary for separating a mixture, and a sample solution sent from the capillary are electrostatically sprayed. An electrostatic spray ion source for generating ions, an ion introduction pore for introducing the generated ions into a vacuum section, and a mass spectrometer including a mass analysis section for performing mass analysis of the introduced ions. Connecting a load with impedance to the spray tubing,
The potential of the spray capillary for performing the electrostatic spraying is maintained at several kilovolts by the potential drop due to the current flowing through the load. More specifically, the potential of the spray capillary is kept constant by changing the impedance of the load according to the value of the current flowing through the load.
【００１３】[0013]
【作用】負荷部を流れる電流による電位降下により噴霧
細管を高い電位に保持するので、静電噴霧用高圧電源が
不要となり、電気回路構成が簡単となる。また、負荷を
流れる電流値に応じてインピーダンスを変えるので、噴
霧細管の電位を一定に保つことができ、従って安定な静
電噴霧が可能となる。Since the spray capillary is kept at a high potential by the potential drop due to the current flowing through the load, a high-voltage power supply for electrostatic spray is not required, and the electric circuit configuration is simplified. In addition, since the impedance is changed according to the value of the current flowing through the load, the potential of the spray tubule can be kept constant, so that stable electrostatic spray can be achieved.
【００１４】[0014]
【実施例】本発明の実施例を図１から図７を用いて説明
する。図１は本発明の実施例を示す電気回路の構成図で
ある。図１０に示した静電噴霧用電源に替わり、負荷16
ａを設ける。この負荷１６ａのインピーダンスと負荷１
６ａを流れる電流Ｉ2 による電位降下により、ステンレ
ス管１０を数キロボルトの電位に保持する。キャピラリ
２の一端に電気泳動用電源１４により高電圧を印加す
る。キャピラリ２中に導入された試料は電気泳動され、
キャピラリ２の他端へと移動する。キャピラリ２の他端
ではシースフローと混合され、噴霧細管１０より、対向
電極１１方向へ静電噴霧される。このように静電噴霧用
電源に替わり負荷１６ａを設ける構成により、静電噴霧
用電源が不要となり、回路構成が簡単になる。また、電
位降下により噴霧細管の電位を一定に保つことができ、
安定に静電噴霧が可能となる。バッファ溶液の組成を変
化させたい場合、すなわち、キャピラリ２部分のインピ
ーダンスが変化する場合や、電気泳動用電源１４の出力
電圧を変化させたい場合には、負荷１６ａとしてインピ
ーダンスが可変な負荷を用い、負荷１６ａのインピーダ
ンスを変えることによりステンレス管１０の電位を調節
しても良い。インピーダンスを調節することによりステ
ンレス管の電位が一定に保たれるので、安定に静電噴霧
を行うことができる。したがって、質量分析計において
イオンを安定に観測することができる。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIGS. FIG. 1 is a configuration diagram of an electric circuit showing an embodiment of the present invention. Instead of the power supply for electrostatic spraying shown in FIG.
a is provided. The impedance of the load 16a and the load 1
The potential drop due to the current I 2 flowing through 6a, holds the stainless steel tube 10 to a potential of several kilovolts. A high voltage is applied to one end of the capillary 2 by the power supply 14 for electrophoresis. The sample introduced into the capillary 2 is electrophoresed,
It moves to the other end of the capillary 2. The other end of the capillary 2 is mixed with the sheath flow and electrostatically sprayed from the spray capillary 10 toward the counter electrode 11. By providing the load 16a instead of the power supply for electrostatic spraying in this way, the power supply for electrostatic spraying becomes unnecessary, and the circuit configuration is simplified. In addition, the potential of the spray capillary can be kept constant by the potential drop,
Electrostatic spray can be stably performed. When it is desired to change the composition of the buffer solution, that is, when the impedance of the capillary 2 portion changes, or when it is desired to change the output voltage of the electrophoresis power supply 14, a load having a variable impedance is used as the load 16a. The potential of the stainless steel tube 10 may be adjusted by changing the impedance of the load 16a. By adjusting the impedance, the potential of the stainless steel tube is kept constant, so that the electrostatic spray can be stably performed. Therefore, the ions can be stably observed in the mass spectrometer.
【００１５】また、図２や図３に示したように、電流計
１７を用いて電流Ｉ0や電流Ｉ2を測定し、あるいは電圧
計１８を設けて噴霧細管１０の電位を測定し、この信号
を信号ライン８ｂを介して負荷１６ａ部に送り、信号に
応じて負荷１６ａのインピーダンスを制御する機構を設
けてもよい。As shown in FIGS. 2 and 3, the current I 0 and the current I 2 are measured by using an ammeter 17 or the potential of the spraying capillary 10 is measured by providing a voltmeter 18. A mechanism that sends a signal to the load 16a via the signal line 8b and controls the impedance of the load 16a according to the signal may be provided.
【００１６】また、図４に示すように、静電噴霧用電源
１５を用いる構成でも、流入する電流Ｉ2 に応じて電源
１５のインピーダンスを調節する機構を設け、ステンレ
ス管１０の電位を保ってもよい。Further, as shown in FIG. 4, even in the configuration using the power supply 15 for electrostatic spraying, a mechanism for adjusting the impedance of the power supply 15 in accordance with the inflowing current I 2 is provided to maintain the potential of the stainless steel tube 10. Is also good.
【００１７】さらに、図５に示すように、負荷１６ａと
静電噴霧用電源１５とを並列に設け、電流計１７あるい
は電圧計１８からの信号に応じてスイッチ１９を切り替
えてもよい。Further, as shown in FIG. 5, a load 16a and a power supply 15 for electrostatic spraying may be provided in parallel, and a switch 19 may be switched according to a signal from an ammeter 17 or a voltmeter 18.
【００１８】キャピラリ２中を流れる電流Ｉ0 の変動に
対して、噴霧細管１０の電位の変動を少なくするため
に、図６に示すように、電源１５により電流Ｉ3 を流
し、電流Ｉ0に比べて大きい電流Ｉ2を負荷１６ａに流し
てもよい。この構成にすることにより、電流Ｉ0が変動
した場合にも安定な静電噴霧が可能となる。The relative variation of the current I 0 flowing in capillary 2, in order to reduce the variation in the potential of the spray capillary 10, as shown in FIG. 6, electric current I 3 by the power source 15, the current I 0 a large current I 2 than may be flowed to the load 16a. This configuration enables stable electrostatic spraying even when the current I 0 fluctuates.
【００１９】また、図７に示すように、負荷１６ｂをキ
ャピラリ２と並列に設けて電流Ｉ3を流してもよい。Further, as shown in FIG. 7, it may be by applying a current I 3 is provided the load 16b in parallel with the capillary 2.
【００２０】[0020]
【発明の効果】本発明によれば、キャピラリ電気泳動と
静電噴霧イオン源とを直結した場合、キャピラリ中を流
れる電流値によらず、噴霧細管の電位を任意の値に保つ
ことができ、従って静電噴霧を安定に行うことができ
る。これにより、イオンを安定に観測できるキャピラリ
電気泳動・質量分析計が提供できる。According to the present invention, when capillary electrophoresis is directly connected to an electrostatic spray ion source, the potential of the spray capillary can be maintained at an arbitrary value irrespective of the current value flowing through the capillary. Therefore, the electrostatic spray can be stably performed. This can provide a capillary electrophoresis / mass spectrometer capable of stably observing ions.
【図１】本発明の実施例である負荷を流れる電流による
電位降下によりステンレス管を一定の電位に保つ静電噴
霧イオン源の電気回路の説明図。FIG. 1 is an explanatory diagram of an electric circuit of an electrostatic spray ion source that maintains a stainless steel tube at a constant potential by a potential drop due to a current flowing through a load according to an embodiment of the present invention.
【図２】本発明の実施例である電流を測定する機構を有
し、電流値に応じてインピーダンスを調節する静電噴霧
イオン源の電気回路の説明図。FIG. 2 is an explanatory diagram of an electric circuit of an electrostatic spray ion source that has a mechanism for measuring current and adjusts impedance according to a current value according to an embodiment of the present invention.
【図３】本発明の実施例である電流あるいは電圧を測定
する機構を有し、電流値あるいは電圧値に応じてインピ
ーダンスを調節する静電噴霧イオン源の電気回路の説明
図。FIG. 3 is an explanatory diagram of an electric circuit of an electrostatic spray ion source which has a mechanism for measuring current or voltage and adjusts impedance according to a current value or a voltage value according to an embodiment of the present invention.
【図４】本発明の実施例である電流に応じて内部インピ
ーダンスを調節する機構を有する静電噴霧用高圧電源を
用いた静電噴霧イオン源の電気回路の説明図。FIG. 4 is an explanatory diagram of an electric circuit of an electrostatic spray ion source using a high voltage power supply for electrostatic spray having a mechanism for adjusting an internal impedance according to a current according to an embodiment of the present invention.
【図５】本発明の実施例である負荷と静電噴霧用高圧電
源とを並列に設け、電流値あるいは電圧値に応じて負荷
と電源とを切り替える機構を有する静電噴霧イオン源の
電気回路の説明図。FIG. 5 is an electric circuit of an electrostatic spray ion source having a mechanism according to an embodiment of the present invention in which a load and a high-voltage power supply for electrostatic spray are provided in parallel and a mechanism for switching between the load and the power according to a current value or a voltage value is provided. FIG.
【図６】本発明の実施例である負荷を流れる電流による
電位降下によりステンレス管を一定の電位に保つ静電噴
霧イオン源の電気回路の説明図。FIG. 6 is an explanatory diagram of an electric circuit of an electrostatic spray ion source that maintains a stainless steel tube at a constant potential by a potential drop due to a current flowing through a load according to an embodiment of the present invention.
【図７】本発明の実施例である負荷を流れる電流による
電位降下によりステンレス管を一定の電位に保つ静電噴
霧イオン源の電気回路の説明図。FIG. 7 is an explanatory diagram of an electric circuit of an electrostatic spray ion source that maintains a stainless steel tube at a constant potential by a potential drop due to a current flowing through a load according to an embodiment of the present invention.
【図８】従来のキャピラリ電気泳動・質量分析計のブロ
ック図。FIG. 8 is a block diagram of a conventional capillary electrophoresis / mass spectrometer.
【図９】従来の静電噴霧イオン源を備えたキャピラリ電
気泳動・質量分析計の断面図。FIG. 9 is a cross-sectional view of a conventional capillary electrophoresis / mass spectrometer equipped with an electrostatic spray ion source.
【図１０】従来のキャピラリ電気泳動・質量分析計の電
気回路の説明図。FIG. 10 is an explanatory diagram of an electric circuit of a conventional capillary electrophoresis / mass spectrometer.
【図１１】従来のキャピラリ電気泳動・質量分析計の電
気回路の説明図。FIG. 11 is an explanatory diagram of an electric circuit of a conventional capillary electrophoresis / mass spectrometer.
１…バッファ槽、２…キャピラリ、１０…ステンレス
管、１１…対向電極、１４…電気泳動用高圧電源、１６
ａ…負荷。DESCRIPTION OF SYMBOLS 1 ... Buffer tank, 2 ... Capillary, 10 ... Stainless steel tube, 11 ... Counter electrode, 14 ... High voltage power supply for electrophoresis, 16
a ... Load.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 平林 集 東京都国分寺市東恋ケ窪１丁目280番地 株式会社 日立製作所 中央研究所内 (56)参考文献 特開 平１−315940（ＪＰ，Ａ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 27/62 - 27/70 G01N 27/447 H01J 49/04 G01N 30/72 ────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Shu Hirabayashi 1-280 Higashi Koikekubo, Kokubunji-shi, Tokyo Central Research Laboratory, Hitachi, Ltd. (56) References JP-A-1-315940 (JP, A) (58) Survey Field (Int.Cl. 7 , DB name) G01N 27/62-27/70 G01N 27/447 H01J 49/04 G01N 30/72
Claims (4)
れ送出されてくる試料溶液を静電噴霧させイオンを生成
する静電噴霧イオン源と、前記静電噴霧イオン源で生成
したイオンを真空部へ導入するためのイオン導入細孔
と、前記導入されたイオンを質量分析するための質量分
析部と、前記試料溶液を噴霧するために前記キャピラリ
の一端に設けられた噴霧細管と、一方の極がアースさ
れ、他方の極が前記キャピラリの前記噴霧細管と反対側
の端部に接続された電圧源と、前記噴霧細管とアースと
の間に接続されたインピーダンスを有する負荷とを有す
ることを特徴とする質量分析計。1. A capillary, an electrostatic spray ion source for electrostatically spraying a sample solution separated and sent out by the capillary to generate ions, and introducing ions generated by the electrostatic spray ion source into a vacuum section. An ion-introducing pore for mass spectrometry, a mass analyzer for mass-analyzing the introduced ions, and the capillary for spraying the sample solution.
And spraying capillary provided at one end of one pole earth
And the other pole is on the opposite side of the capillary to the spray capillary.
A voltage source connected to an end of, and the spraying capillary and the ground
And a load having an impedance connected between the mass spectrometer.
可変である質量分析計。2. The mass spectrometer according to claim 1, wherein said impedance is variable.
て、前記キャピラリ中を流れる電流値、前記負荷を流れ
る電流値、あるいは前記噴霧細管の電位のうち少なくと
も一つを測定し、前記電流値、あるいは前記電位に応じ
て前記インピーダンスを変える質量分析計Te wherein any one smell of claim 1 or 2 <br/>, current flowing in the capillary, measuring at least one of the potential of the current value or the spraying capillary flow through said load And a mass spectrometer that changes the impedance according to the current value or the potential
備えたことを特徴とする請求項１から３のいずれか一項
に記載の質量分析計。Wherein said mass spectrometer as claimed in any one <br/> of claims 1 to 3, characterized in that it comprises a measuring means for measuring the potential of the spray capillary.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03963993A JP3336659B2 (en) | 1993-03-01 | 1993-03-01 | Mass spectrometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP03963993A JP3336659B2 (en) | 1993-03-01 | 1993-03-01 | Mass spectrometer |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002000978A Division JP3654246B2 (en) | 2002-01-08 | 2002-01-08 | Mass spectrometer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH06249833A JPH06249833A (en) | 1994-09-09 |
| JP3336659B2 true JP3336659B2 (en) | 2002-10-21 |
Family
ID=12558669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP03963993A Expired - Lifetime JP3336659B2 (en) | 1993-03-01 | 1993-03-01 | Mass spectrometer |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3336659B2 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2746928A1 (en) | 2009-02-17 | 2010-08-26 | Medimate Holding B.V. | An apparatus for the measurement of a concentration of a charged species in a sample |
| WO2014099672A1 (en) | 2012-12-21 | 2014-06-26 | Andrew Hancock | System and method for multipath processing of image signals |
| JP2019536985A (en) * | 2016-09-26 | 2019-12-19 | ユニヴァーシティー オブ ノートル ダム デュ ラック | Method and apparatus for mitigating current reversal in a capillary zone electrophoresis / electrospray device |
-
1993
- 1993-03-01 JP JP03963993A patent/JP3336659B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH06249833A (en) | 1994-09-09 |
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