JP3314189B2 - Method for diagnosing the activity of wastewater treatment organisms - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method for diagnosing the activity of wastewater treatment organisms.

[0002]

2. Description of the Related Art Conventionally, a biological treatment method has been widely used as a method for decomposing and removing human waste, sewage, various industrial wastewater, and the like. However, the biological treatment method has advantages in running cost and the like as compared with the physical treatment method and the chemical treatment method, but has a problem in the instability of treated water quality. This causes the activity of wastewater treatment organisms (hereinafter called organisms) to be lost,
This is due to fluctuations in environmental factors (organic load, pH, water temperature, inflow of growth-disturbing substances such as heavy metal salts, acids, and alkalis) that cause runoff or death outside the treatment system. For this reason, in order to obtain stable treated water quality, it is necessary to constantly monitor for changes in environmental factors, and it is necessary to perform maintenance based on the results.

[0003] One of the methods is a feedforward method. This measures the physical temperature, pH, alkalinity, electrical conductivity, turbidity, etc. of the wastewater, controls the coagulant injection based on this, and monitors the floc of the activated sludge method. is there. However, in this method, control cannot be performed by directly reflecting the state of floc formation, and it is said that this method lacks certainty.

[0004] In recent years, a monitoring system based on Flock's online image recognition has been devised. However, there are many problems because accuracy problems and application range are limited to large-scale processing facilities, and practical use still requires time.

[0005] Therefore, in the current treatment facility, maintenance is still carried out by visual observation, water quality analysis, observation of micro-animals serving as indices, and the like, and judgment of a technical expert based on the results. .

[0006]

In general, there is a difference between the activity of the organism itself and the treatment function of decomposing and removing wastewater.
There is a close relationship. That is, the organism whose activity has been enhanced by appropriate maintenance can efficiently perform the decomposition and removal of the wastewater. Therefore, if biological activity can be grasped in advance, future processing performance can be predicted,
Optimal maintenance is possible.

However, in the prior art, since the biological activity is predicted on the basis of the results of water quality analysis and the like, grasping the actual state of living organisms must be indirect. For example,
As a result of water quality analysis, even if no abnormality is found, there are often cases where the water quality changes suddenly the next day, the biological activity is lost, and good water quality cannot be obtained. That is, it is difficult for the conventional technology to grasp the actual state of the organism under a chronic stress environment. In this sense, it is desired to establish a technique for measuring biological activity more directly.

In the prior art, since it takes time to obtain an analysis result from sampling, there are cases where maintenance is delayed.

Furthermore, if the analysis items become widespread, a great deal of labor and various specialized knowledge are required, so that it is difficult for anyone to easily judge the processing status.

[0010] The present invention has been made in view of the above problems, and has as its object to provide a method for directly and quickly grasping the biological activity for the purpose of performing appropriate maintenance.

[0011]

Means for Solving the Problems The present invention provides a method for rapidly and simply using an activity diagnostic agent produced by an organism growing in a biological treatment facility based on a stress protein (hereinafter referred to as HSP) synthesized in a stress environment. It is concerned with a method of grasping the activity and performing appropriate maintenance.

First, the stress response which is the principle of the present invention will be described. All living cells, from microorganisms to higher plants and animals, instantly synthesize HSP when exposed to environmental stresses (high temperature, low temperature, hydrostatic pressure, drying, ultraviolet rays, etc.), and establish a system that can withstand these environments. This is called a stress response. For example, when a yeast cell is placed in a temperature environment which is ten and several degrees higher than the optimum growth temperature, it rapidly synthesizes a kind of HSP called heat shock protein and acquires resistance to lethal high temperature. Further, it has been revealed that not only high temperature resistance but also pressure resistance, drying resistance, frost resistance and high concentration oxygen resistance can be obtained by the same heat shock treatment. Since the stress response is a mechanism common to all organisms, a wide variety of organisms involved in biological treatment: bacteria, fungi,
Algae, protozoa, metazoans and the like also sense environmental changes as stress, and synthesize HSP at that time. The present invention utilizes the stress response of these organisms.

Next, an activity diagnosis method using this stress response will be described. (1) Acquisition of HSP of organisms collected from existing biological treatment facilities. (2) Acquisition of an activity diagnostic agent based on the above. (3) Application to biological treatment facilities. Is divided into three stages. Hereinafter, description will be made in accordance with this.

(1) Acquisition of HSP An organism collected from an existing biological treatment facility is exposed to a stress environment such as low temperature, low pH, and no load in a laboratory experiment, and the specific HSP synthesized at this time is subjected to electrophoresis. After conducting an experiment to confirm the reproducibility of HSP synthesis by a technique such as separation, HSP is purified and obtained by a technique such as dialysis. In addition, these living organisms include activated sludge and biofilm, but the present invention is not limited thereto. (2) Preparation of an activity diagnostic agent To prepare an activity diagnostic agent, purified HSP is injected into the blood of a laboratory animal such as a rat or rabbit, and several weeks later, the collected blood is purified to obtain an antibody. A label such as a fluorescent substance is added to this, so that when the HSP of the biological treatment facility comes into contact with the antibody, the presence thereof is instantly recognized. In the present invention, this is treated as an activity diagnostic agent. (3) Application to biological treatment facilities Organisms from a biological treatment facility for activity diagnosis are collected and contacted with an activity diagnostic agent created in advance. When these organisms are exposed to a stress environment, the activity diagnostic agent responds sharply, so that the state of decreased activity can be quickly identified. Based on this result, quick and appropriate maintenance can be performed. As another method, an active diagnostic agent can be incorporated into a processing facility as a molecular recognition element, and a maintenance system by automatic control can be performed. That is, an active diagnostic agent is immobilized in a treatment facility as a molecular recognition element, and an antigen-antibody reaction with HSP is constantly measured by a device. The measurement result is converted into an electric signal, based on which the water temperature, pH, coagulant injection and the like are automatically controlled, and an optimal state can be maintained.

[0015]

Embodiments of the present invention will be described below. There are various stresses to which the organisms of the biological treatment facility are exposed. In the examples of the present invention, starvation was taken out, and at this time, HSP to be synthesized was confirmed.

Activated sludge acclimated to artificial sewage media having the composition shown in Table 1 was collected, centrifuged, adjusted to a sludge concentration of about 2000 mmg / l, and batched in the same media. The culture experiment was performed for 144 hours. The culture temperature was 23 ° C. Activated sludge was collected over time from the start of culturing, and this was separated into a supernatant portion and a sludge portion by centrifugation. The supernatant is used to measure the concentration of organic carbon (hereinafter referred to as TOC), thereby confirming the fate of the substrate,
It was used as an indicator of whether or not a person was starving.

[0017]

[Table 1]

After adding a trisodium decyl sulfate buffer to the sludge portion, the molecular weight cutoff of the protein was determined by polyacrylamide electrophoresis, and the HSP
Was confirmed.

FIG. 1 shows the results of the change over time in TOC. Thereby, the TOC in the substrate was quickly consumed by the activated sludge, and the TOC after 24 hours of culture was all derived from inorganic carbon. Therefore, it is considered that this activated sludge was starved after 24 hours of culture. . For this reason, we will focus on the proteins during this culture period.

FIG. 2 (schematic diagram of the photograph) shows the change of protein in activated sludge up to 144 hours of culture. As a result, culture 9
At a molecular weight of about 29 kilodaltons at 6 hours and 144 hours, a protein having a molecular weight not observed in other culture periods was observed. As a result of these two-dimensional isoelectric focusing, the distribution of the molecular weight cut-off of the protein at 96 hours and 144 hours was different from those in other culture periods.

From the above examples, it was confirmed that all substrates were consumed by batch culture, and activated sludge under starvation synthesized a specific protein.

[0022]

The activity diagnostic agent according to the present invention enables quick and simple diagnosis of the activity of an organism and enables maintenance and management of an appropriate biological treatment facility. In addition, by combining the present invention with a conventional water quality analysis or the like, maintenance by automatic control can be constructed.

[Brief description of the drawings]

FIG. 1 is a diagram showing a time-dependent change in TOC.

FIG. 2 is an explanatory diagram showing a change in protein of activated sludge.

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Claims (1)

(57) [Claims] The present invention provides a wastewater treatment organism that grows in a biological treatment facility such as night soil, sewage, and various industrial wastewaters by applying a stress environment to the organism to synthesize a stress protein, and to separate and purify the protein. After injecting the protein into the blood of the experimental animal, the collected blood is purified,
A method for diagnosing the activity of a wastewater treatment organism, comprising obtaining an antibody against the stress protein, labeling the antibody to prepare an activity diagnostic agent, and reacting the activity diagnostic agent with the wastewater treatment organism.