JP3314148B2 - Trunk injection - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a trunk injection and a method for protecting trees using the stem injection. More specifically, a trunk injection comprising an insecticidal or bactericidal active ingredient and a surfactant, particularly containing essential oils to enhance and sustain the activity of the active ingredient, and a trunk injection comprising the essential oil. The present invention relates to a method for protecting trees by injecting an agent into a trunk.

[0002]

2. Description of the Related Art Trunk injectants have conventionally been used to effectively prevent tree (especially pine) mortality due to pests in trees. The stem-injected drug is absorbed from the roots, dissolves in water in the tree body that moves to the leaves through the tentative conduit, and migrates to branches, etc. Is required for its solubility in water. In this regard, Matsuura reports that only agents with a solubility in water of more than 1000 ppm showed efficacy against pine blight by stem injection. (Plant Protection, 38, 27-31, 1984). For this reason,
If the solubility in water is low, a considerable amount of the drug precipitates around the injection site, which inhibits the conduction of water,
As a result, it is said that the effect on pests in the tree is not stably maintained. As a method for solving this point, a solubilized preparation has been proposed. (Japanese Patent Laid-Open No. 8-175
However, many of these active ingredients that are active against pests have poor stability. Especially in water, the active ingredient is hydrolyzed and disappears. It is liable to occur, resulting in a drawback that sufficient sustainability of the effect cannot be obtained. Further, depending on the compound, there are also active ingredients that cause phytotoxicity to trees when the concentration of the active ingredient in the tree is too high. For this reason, it is, of course, necessary to spread the active ingredient at an early time after injection so that the active ingredient has a concentration necessary and sufficient to exert biological activity, but all the active ingredient is diffused throughout the tree at a time. Doing so often comes at a disadvantage. Rather,
The remaining active ingredient does not precipitate crystals around the injection point,
It is advantageous from the viewpoints of sustained efficacy and phytotoxicity to store in a state free from phytotoxicity, gradually dissolve in the stem flow as needed, and release it into the stem flow. Also, for example, pine wood nematodes are not only found in the stem water of pine trees but also survive in the woody part, so even if the active ingredient is simply dissolved in the stem flow and the active ingredient is present only in the water, it survives in the woody part In some cases, the effect cannot be exerted on a nematode that does not have sufficient effects.

[0003]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a stem injecting agent which solves the problems of the conventional stem injecting agent, has a long duration of efficacy, and has little phytotoxicity.

[0004]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies in order to achieve such an object. As a result, immediately after the injection, the concentration of the active ingredient in the tree was increased to the concentration required for biological activity. After that, do not raise more than necessary,
By gradually releasing the active ingredient into the tree, the concentration in the tree was maintained at a biologically necessary concentration without causing phytotoxicity over a long period of time, and the present invention was completed.

[0005]

DETAILED DESCRIPTION OF THE INVENTION That is, the present invention provides a trunk injection comprising (1) an insecticidal or fungicidal active ingredient, (2) an essential oil, and (3) a surfactant. By injecting the trunk injection agent into the trunks of pine and the like and translocating them into the tree, the pine wood nematode can be maintained at a biologically required concentration for a long time without causing phytotoxicity. And other various pests.

The species of the tree is not particularly limited. For example, pine, cedar,
Not only forest trees such as cypress, but also citrus fruits, apples, pears, figs, persimmons, peaches, grapes, chestnuts, cherry peaches, plums, prunes, loquats, gummy, plum-like fruit trees, sarsberg, sasanqua, camellia, mukuge, cherry blossoms It is a pest that occurs in garden trees such as flowering trees, oaks, oaks, oaks and maples.

[0007] Pests, for example, breed in trees such as pine wood nematodes, piercing pests that penetrate and harm trees such as longhorn beetles, bark beetles, scuttles and scabbards, and block the flow of sap. Insect pests such as nematodes, aphids, scale insects, rotifers, stink bugs, snails, snails, bugs, bugs, insects such as wasps, pests such as spider mites, and udon. Disease, sunspot,
Diseases such as scab, scab, rot, anthracnose, gray scab, canker and downy mildew can be mentioned.

Examples of the pesticidal active ingredient effective for controlling these pests include organophosphorus pesticides such as isoxathion, ethylthiomethone, fenitrothion, prothiophos, propaphos, phosthiazate, and pyraclophos, and benzoic acids such as RH-7988. Carbamate insecticides such as imidazole anthelmintics, oxamil, mesomil, benflacarb, synthetic pyrethroid insecticides such as permethrin, cypermethrin, etofenprox, silafluofen, insect growth regulators such as chlorfluazuron, flufenoxuron Acaricide, milbemycin A, such as
₃ , A ₄ , α ₁₁ , α ₁₄ , milbemycin 13-position derivative,
Avermectin B ₁ a, B ₁ b, nemadectin, macrolide insecticidal and acaricidal agents, such as moxidectin, imidacloprid, nitenpyram, and a neonicotinoid insecticides such as acetamiprid.

The common names of "milbemycins", "avermectins" and "nemadectins" and their structures described in the claims of the present invention are listed below.

[0010]

Embedded image

[0011] milbemycin A _3, A ₄ 56-45890 JP-B for, JP-B-56-46791, for milbemycin D is in Kokoku No. 2-17555, milbemycin alpha _11, for alpha ₁₄ is KOKOKU 1 −
No. 193,270, in Kokoku No. 7-8871, avermectin B ₁ a, the B ₁ b is JP 52-151197
And JP-A-53-130695.
No. 293, for nemadectin, see JP-A-61-10.
No. 589, Moxidectin is disclosed in JP-A-63-11 / 1988.
No. 9486 and JP-A-62-265288.

The 13-position derivative of milbemycin refers to the derivative disclosed in JP-A-5-255343, and particularly, 13- [2-phenyl-2- (2-pyrimidinylthio) acetoxymilbemycin A ₄ and 13- [ shows a 2-phenyl-2- (2-pyrimidinylthio) acetoxy milbemycin a _3. Also, European Patent Publication No. 67
5133 shows the derivatives disclosed in US Pat.
(Α-methoxyiminophenylacetoxy) milbemycin A ₄ and 13- (α-methoxyiminophenylacetoxy) milbemycin A ₃ are shown.

Examples of the fungicidal active ingredient include triazole fungicides such as triadimenol and propiconazole, polyhaloalkylthio fungicides such as captan, benzimidazole fungicides such as benomyl and thiophanate methyl. Metalaxyl, acylalanine fungicides such as oxadixyl, pyrifenox,
Examples include ergosterol biosynthesis inhibitors such as prochloraz and antibiotics such as kasugamycin, polyoxin and streptomycin. These pesticidal or bactericidal active ingredients can be used alone or in combination of two or more.

The amount of these active ingredients in the trunk injection varies greatly depending on the type and physical properties of the active ingredients.
Usually about 0.01 to 50%, preferably 0.1 to 3%.
It is about 0%.

The essential oils used in the present invention are volatile oils obtained from various plants and each having a unique aroma.
It is insoluble in water and generally soluble in organic solvents. It can be obtained by steam distillation from various plants, and its components are usually composed of several compounds, the main ones being terpene or aromatic hydrocarbons, alcohols, aldehydes and ketones, phenols, various esters, etc. is there. Typical essential oils include turpentine oil, camphor oil, lemon oil, bergamot oil, coconut oil, peppermint oil, citronella oil, melissa oil, eucalyptus oil, lemongrass oil, orange peel oil, wintergreen oil, sweet birch oil , And these distillation purification sections.

These essential oils are originally present in plants, have good affinity with plants, are unlikely to cause phytotoxicity to plants, and have the property of dissolving many organic substances. In addition, these essential oils are often composed of relatively low-polarity compounds, and in many cases, even if the active ingredient is susceptible to hydrolysis, it can be stably maintained. It is not clear by what mechanism the trunk injection agent formulated using these essential oils exerts the effects of the present invention, but in view of the above-mentioned properties of essential oils, the following mechanism is considered. Conceivable.

In other words, when the trunk injection agent of the present invention is injected into the stem, some of the active ingredients dissolve in the stem flow and spread throughout the tree along the flow of water, but dissolve in the stem flow. The remaining active ingredient that did not remain either dissolved in these essential oils stops near the injection point or moves in the tree by riding on the resin flow, and thereafter repeatedly distributes with the stem flow, gradually removing the active ingredient from the stem flow. It seems to play a role in releasing into.

It is desirable to use essential oils as far as they can dissolve the active ingredient in a sufficient amount. Therefore, the amount of these essential oils is generally 1 to 90%, preferably 1 to 90%.
About 70%, more preferably about 2 to 60%. By increasing or decreasing the blending amount of the essential oil, the amount of the active ingredient that moves into the tree by riding on the stem flow immediately after the injection can be adjusted to some extent.

However, since many of these essential oils are hardly soluble in water, merely dissolving the active ingredient in the essential oil hardly dissolves the injected active ingredient in the stem flow. For this reason, the active ingredient ends up not reaching the effective concentration in the tree. In order to raise the initial active ingredient concentration in the tree to a biologically effective concentration, it is essential to use a surfactant. The surfactant may be any of nonionic, anionic, cationic and amphoteric surfactants, and is not particularly limited.

Suitable nonionic surfactants include polyoxyethylene alkyl allyl ether, polyoxyethylene allyl ether, polyoxyethylene alkyl ether, polyoxyethylene alkyl ester,
Sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, copolymer of ethylene oxide and propylene oxide, polyoxyethylene castor oil ether, polyoxyethylene hydrogenated castor oil ether, sucrose fatty acid ester, and nonionic surfactant alkylene oxide having the above alkylene oxide Phosphate- or sulfate-terminated ones or salts thereof can be mentioned. Examples of the anionic surfactant include alkyl benzene sulfonates, alkyl sulfates, dialkyl sulfosuccinates, higher fatty acid salts, and the like. Examples of the cationic surfactants include alkyl amines, quaternary ammonium salts, and alkyl pyridium. Salts and the like and amphoteric surfactants include amino acid type and betaine type.

Of these, usually, a suitable nonionic surfactant is used alone, or a mixture of these with an anionic surfactant is used. Among the nonionic surfactants, polyoxyalkylene (poly) styrenated phenol, polyoxyalkylene (poly) styrenated cresol and their sulfates or phosphates or salts thereof are the best surfactants. (Here, alkylene indicates ethylene or propylene, and (poly) indicates that it is substituted with one or more styrene groups.) These surfactants may be used alone or as a mixture. Depending on the type and amount of these surfactants, the amount of the active ingredient that moves into the tree by riding on the stem flow immediately after injection can be adjusted to some extent. The amount of the surfactant is generally about 0.1 to 50%, preferably 1 to 30%, and more preferably about 5 to 25%.

The stem injecting agent of the present invention may contain, as necessary, other components such as water and other organic solvents, various stabilizers, potency enhancers, fertilizer components, and trace elements having an activating effect on plants. And the like can also be blended.

The organic solvent used in the present invention is used as needed to raise the initial effective ingredient concentration in the tree to an effective concentration. Therefore, these are preferably solvents that are easily miscible with water, for example, methyl alcohol, ethyl alcohol, propyl alcohol, butyl alcohol, lower alcohols such as hexyl alcohol, ethylene glycol, propylene glycol, diethylene glycol and esters and ethers thereof. Glycols and derivatives thereof, cyclohexanone, acetone, ketones such as methyl ethyl ketone,
Esters such as ethyl acetate and butyl acetate; sulfoxides such as dimethyl sulfoxide (hereinafter abbreviated as DMSO); glycerin and its esters and glycerin derivatives such as ether; N-methylpyrrolidone and N-ethylpyrrolidone Pyrrolidones, nitriles such as acetonitrile, tetrahydrofuran,
Examples include ethers such as dioxane, and preferably, glycols, ketones, esters,
Sulfoxides, more preferably ketones, particularly preferably cyclohexanone. Depending on the type and the amount of the organic solvent, the amount of the active ingredient that moves into the tree by riding on the stem flow immediately after the injection can be adjusted to some extent. The amount of the organic solvent varies depending on the type and the amount of the active ingredient, but generally ranges from 0 to 9.
It is about 0%, preferably about 10 to 80%, and more preferably about 30 to 80%. The stabilizer is added as necessary to maintain the stability of the active ingredient in the preparation or after injection of the trunk. For example, acids, pH adjusters such as alkali, BHT, butylhydroxyanisole, antioxidants such as ascorbic acid, isopropyl citrate, tocopherol, propyl gallate, EDTA, dimethylglyoxime, dithizone, oxine, acetylacetone, glycine, Chelating agents such as nitrilotriacetic acid, and ultraviolet absorbers such as salicylic acid derivatives, hydroxybenzophenone derivatives, benzoic acid derivatives, cinnamic acid derivatives, and coumarin derivatives can be used. The amount of these stabilizers is usually 0 to 5%, preferably 0 to 1%.

The potency enhancer is blended so as to sufficiently exert the activity of the active ingredient. For example, piperonyl butoxide can be used. These blending amounts are usually 0
-30%, preferably about 0-20%.

The incorporation of a fertilizer component or a trace element having an activating effect on plants into a stem injection is effective for activating trees weakened by disease and insect damage. Such components include the three major elements of fertilizers such as nitrogen, phosphoric acid and potassium, as well as trace elements such as calcium, sulfur, zinc, copper, molybdenum, boron, iron, manganese, magnesium and various vitamins. . The amount of these compounds is usually 0 to 30%, preferably 0 to 20%, more preferably 0 to 30%.
About 10%.

The stem injection thus obtained contains 0.1 to 50% of an insecticidal or fungicidal active ingredient and 1 to 90% of essential oils.
%, And a surfactant containing 0.1 to 50% of a surfactant as an essential component.

The trunk injection is prepared by uniformly dissolving each of these components. The preparation may be performed by mixing and dissolving the whole amount in a tank of an appropriate size using a mixer, but in an unstable system in which crystals are likely to precipitate at low or high temperatures, the injection point is set as described above. Since the active ingredient may be crystallized in the vicinity, it is necessary to have sufficient stability against temperature.

For application to a tree, the trunk injection agent of the present invention can be achieved by placing it in a suitable container and injecting it through an injection hole formed in the tree. The injection method may be either natural injection or pressure injection.

The container may be made of an appropriate material such as synthetic resin, glass or metal, but may be made of synthetic resin in consideration of weight, crack resistance, ease of empty bottle processing, pressure resistance, and the like. Are advantageous. It has the same outer diameter as the diameter of the injection hole and has a length of 5
Those having an inlet on the order of cm are advantageous.

The application rate of the trunk injection can be appropriately changed depending on the size, timing, age, damage status, etc. of the tree.

Hereinafter, embodiments of the present invention will be described in more detail with reference to Examples and Test Examples, but it goes without saying that the present invention is not limited to these.

[0032]

Examples 1 to 5 Milbemycin (a mixture of milbemycin A ₃ : A ₄ = 3: 7) was prepared according to the formulation shown in Table 1.
And a stem injection containing 5%.

[0033]

Example 6 Compound 1 was used in an amount of 2.0 according to the formulation shown in Table 2.
% Was obtained.

[0034]

Example 7 10% of compound 2 was prepared according to the formulation shown in Table 2.
A stem infusion containing was obtained.

[0035]

Example 8 A trunk injection containing 10% of ethylthiomethone was obtained according to the formulation shown in Table 2.

[0036]

Example 9 A trunk injection containing 10% isoxathion was obtained according to the formulation shown in Table 2.

[0037]

Example 10 According to the formulation shown in Table 2, a trunk injection containing 2% of pyrimidifen was obtained.

[0038]

Examples 11 to 13 Milbemectin, milbemycin α ₁₄ and compound 1 were added in the proportions shown in Table 3 by 2% each.
A stem infusion containing was obtained.

[0039]

Comparative Examples 1 to 3 According to the formulations shown in Table 1, trunk injections containing 2 and 5% of milbemycin were obtained.

[0040]

[Table 1] Note ¹⁾ Calcium dodecylbenzenesulfonate ²⁾ Sodium dodecylbenzenesulfonate ³⁾ Polyoxyethylene (8 mol) nonyl phenyl ether ⁴⁾ Polyoxyethylene (65%) tristyryl phenyl ether ⁵⁾ Polyoxyethylene (10 mol) oleyl Ether ⁶⁾ Polyoxyethylene (33 mol) castor oil ether ⁷⁾ Polyoxyethylene (40 mol) hydrogenated castor oil ether

[0041]

Table 2 Numerical values indicate% by weight. ──────────────────────────────────── Example No. 6 7 8 9 10 Compound 1 2.0 Compound 2 10.0 Ethyl thiomethone drug substance 10.0 Isoxathion Active ingredient 10.0 Perimidisifen Active ingredient 2.0 Terepine oil 55.0 26.0 58.0 Camphor white oil 20.0 Limonene 55.0 Cyclohexanone 13.0 10.0 Phthyl acetate 40.0 Polyethylene glycol 30.0 DMSO 20.0 NE2607 ⁸⁾ 15.0 NE707 ⁹⁾ 16.0 NE610 ¹⁰⁾ 25.0 15.0 TP6618 ¹¹⁾ 5.0 5.0 DB 4.0 NE707SF ¹²⁾ 15.0 ────────────────────────────────── Total 100.0 100.0 100.0 100.0 100.0 ───── ─────────────────────────────── Note) Compound 1: 13- (α- Toki Castanopsis Mino phenylacetoxy) Miruhe Bu mycin A4 Compound 2: (RS) -2 (4- fluorophenyl) -1-(1H chromatography 1,2,4 Toriaso Bu Ru 1-yl) -3 chromatography trimethylsilyl-safe ° Loja ° Hmm 2-ol ⁸⁾ polyoxyethylene (59% of intramolecular ethylene oxide) tristyryl phenyl ether ⁹⁾ polyoxyethylene (61.5% of intramolecular ethylene oxide) distyryl cresyl ether ¹⁰⁾ polyoxyethylene (69% of intramolecular ethylene oxide) distyryl phenyl ether ^{11 )} Polyoxyethylene (9 mol) oleate ¹²⁾ Sulfate ammonium salt of NE707

[0042]

[Test Example 1] The trunk injections shown in Examples 1 to 5 and Examples 11 to 13 and Comparative Examples 1 to 3 were applied to 5th-year-old red pine.
0.05 g was injected as an active ingredient per bottle. One month after 1 month, 1 year and 2 years, highly toxic pine wood nematode suspension (100,000 heads / ml)
0.3 ml was inoculated, and three months later, the effect of preventing death was examined. Some trees are 1 month, 3 months, 1 year, 2
One year later, the most distal node extending from the main trunk was cut, and the concentration of milbemycin contained in the section was measured. The results are shown in Table 4. The number of dead trees is the number of dead trees in 10 trees per section, and the concentration in the main trunk is the concentration of milbemectin (pp.
m).

As shown in Table 4, each of the trunk injections of the present invention exhibited a more stable mortality prevention effect than the preparation of Comparative Example. The active ingredient concentration at the tip of the main trunk was determined in Comparative Examples 1 and 2.
In the formulation of the present invention, the value was relatively high in the early stage, but then rapidly decreased. In addition, in the preparation of Comparative Example 3, the initial concentration was low, and it seems that the effective concentration did not reach the effective concentration throughout the period. On the other hand, in the preparation of the present invention, the initial concentration of the active ingredient was equal to or slightly lower than that of Comparative Examples 1 and 2, but no sharp decrease was observed after that, and the effective concentration was 2 years later. Maintained. There were no signs of phytotoxicity in any of the plots.

[0044]

[Table 3] { Example 11 12 13} ─────────────────────────────── Miruhe Bu Mekuchin conformal 2.0 Miruhe Bu mycin alpha ₁₄ conformal 2.0 compound 1 2.0 turpentine oil 3.0 3.0 3.0 methyl Alcohol 67.0 67.0 67.0 Ethylene glycol 15.7 15.7 15.7 NE2609 12.0 12.0 12.0 BHT 0.1 0.1 0.1 Lactic acid 0.2 0.2 0.2 ───────────────────────────── ───────

[0045]

[Table 4] Example Sample No. 1 2 3 4 5 11 12 13 数 Number of dead trees (after 1 month) 0 0 0 0 0 0 0 0 (1 year later) 0 0 0 0 0 0 0 0 (2 years later) 0 0 0 0 1 0 0 0 ────────────────────濃度 Main trunk concentration (after 1 month) 0.43 0.62 0.39 0.65 0.29 0.78 0.69 0.70 (after March) 0.68 0.73 0.55 0.63 0.49 0.85 0.75 0.82 (after 1 year) 0.71 0.69 0.70 0.77 0.82 0.89 0.75 0.79 (2 years later) 0.41 0.38 0.46 0.55 0.40 0.53 0.60 0.66 ───────────────────────────── ───────

[0046]

[Test Example 2] Examples 1 and 2 each corresponding to 1.2 g as an active ingredient were applied to Japanese red pine having a breast height of 10 to 15 cm.
The stem injections of 5, 6, and 10 to 13 and Comparative Examples 1 and 3 were injected (March 15). After confirming the yield of the sap on June 10, 0.5 ml of the highly toxic pine wood nematode suspension (100,000 heads / ml) was inoculated at a position of about 2.5 m in height, and 3 months later the effect of preventing mortality Was examined. On June 20 of the following year, the surviving trees were placed at a height of about 2.5 m at a height of a highly virulent pine wood nematode suspension (100,000
ml) was inoculated, and three months later, the effect of preventing death was examined. Table 5 shows the results.

[0047]

[Table 5] ────────────────────────────────────Sample name test for the first year and the third year for the next year Number of trees Number of dead trees Number of test trees Number of dead trees Number of surviving trees ────────────────────────────────── Example 1 1 0 10 0 10/10 Example 2 10 0 10 0 10/10 Example 5 10 0 10 19/10 Example 6 10 00 10/10 Example 10 10 0 10 1 9/10 Example 11 10 0 10 0 10/10 Example 12 10 0 10 0 10/10 Example 13 10 0 10 0 10/10 {Comparative Example 1 10 19 9 5 4/10 Comparative Example 3 10 9 11 10/10} ── ─────────────── As shown in Table 5, trunk injection agent of the present invention, both compared with the preparation of Comparative Example showed a stable mortality prevention effect. There were no signs of phytotoxicity in any of the plots.
Two years later, each tree was inoculated with the spider mite, and one month later, the damage of the spider mite was examined. In Examples 1, 2, 5, 6, 10 to 13, spider mite was observed in all the trees. However, in Comparative Examples 1 and 3, many spider mite infestations were observed.

[0048]

[Test Example 3] 10 ml of the trunk injection agent of Example 7 was injected into a crabgrass tree having a diameter of 8 cm and a height of 3 m (April 20)
Day). Powdery mildew occurred in nearby trees in July, but none of the injected trees. There was no harm to the injected wood.

[0049]

Test Example 4 0.5 ml of the trunk injection agent of Example 8 was injected into a 4 cm diameter plum tree (February 20). Trees that did not inject the stem injectant developed aphids in May,
Although soot developed thereafter, the injected trees did not develop aphids until the fall of the year. There was no harm to the injected wood.

[0050]

Test Example 5 20 ml of the trunk injection of Example 9 was injected into a 7 cm-diameter Unshu mandarin tree (February 20). Trees that had not been injected with the stem injectant had a large amount of wild scale insects, but did not show up throughout the year. There was no harm to the injected wood.

[0051]

According to the present invention, a relatively insoluble active ingredient, which has been unstable in the past, can exhibit a stabilized effect by blending essential oils in the composition. It is a trunk injection. ADVANTAGE OF THE INVENTION By this invention, it became possible to obtain the trunk injection | pouring agent which has an effect lasting over a long period of time and has no fear of a chemical injury. ADVANTAGE OF THE INVENTION According to this invention, since a worker can process a chemical | medical agent easily, without touching a chemical | medical agent directly, there is no concern about a user's health, and the contribution in agriculture / forestry and natural environment protection is large. .

────────────────────────────────────────────────── ─── Continuation of the front page (56) References JP-A-7-149607 (JP, A) JP-A-7-165514 (JP, A) JP-A-7-173017 (JP, A) JP-A 8- 175914 (JP, A) JP-A-61-64403 (JP, A) JP-A-63-68501 (JP, A) WO 95/20877 (WO, A1) (58) Fields investigated (Int. Cl. ⁷⁾ A01N 43/90 A01N 25/00

Claims (7)

(57) [Claims] (1) Milbemycin A ₃ , A4, D, α11,
selected from α14 and the 13-position derivative of milbemycin
One or more pesticidal active ingredients, (2) turpentine oil, camphor oil
A trunk injection containing at least one selected essential oil and (3) a surfactant. 2. The surfactant is at least one selected from polyoxyalkylene (poly) styrenated phenol, polyoxyalkylene (poly) styrenated cresol, and their sulfates or phosphates or salts thereof. Item 6. The trunk injection according to Item 1 . 3. A trunk injection agent according to claim 1 or 2 containing an organic solvent. 4. An organic solvent comprising lower alcohols, glycols and derivatives thereof, ethers, ketones, esters, sulfoxides, nitriles, pyrrolidones,
The trunk injection according to claim 3 , which is at least one member selected from glycerins. 5. The trunk injection according to claim 3 , wherein the organic solvent is cyclohexanone. 6. A method for protecting trees, wherein the tree trunk injection agent according to any one of claims 1 to 5 is injected into the trunk or root of a tree and translocated into the tree to control pests. 7. The method for protecting a tree according to claim 6 , wherein the standing tree is a pine tree and a target to be exterminated is a pine wood nematode.