JP3278928B2 - How to extract body fluid of silkworm - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for extracting body fluids of silkworms for extracting useful substances such as interferon (IFN) produced in the body fluids of the silkworms.

[0002]

2. Description of the Related Art In recent years, studies have been conducted on infecting silkworms with a gene-incorporated virus, growing the virus in the body fluid of the silkworm, and producing useful substances such as interferon used for treating cat viral diseases. ing.

[0003] In order to extract useful substances produced in the body fluid of the silkworm, the body fluid of the silkworm is extracted. As a method of extracting the body fluid, conventionally, the skin of the silkworm is wounded with a knife or the like, and the wound is cut off. A method of squeezing out bodily fluids, a method of incising silkworms with a laser to squeeze out bodily fluids, and a method of piercing small holes in the epidermis of silkworms with a needle to squeeze out bodily fluids are employed.

[0004]

However, the use of such a method has the following problems. In other words, in the method of scratching the epidermis of the silkworm with a knife or the like, it is difficult to make the shape such as the length or depth of the cut constant, and as such,
If the shape of the cut varies depending on the silkworm, there is a problem that the amount of body fluid extracted differs depending on the silkworm, and wasteful extraction of the body fluid is likely to occur. In addition, there is also a problem that when the silkworm is damaged, bodily fluid of the silkworm adheres to the knife and the sharpness of the knife becomes poor, thereby shortening the life of the knife. Furthermore, if a knife is repeatedly used for a plurality of silkworms, there is a risk that the disease may be transmitted between the silkworms, and there is a problem that it is not suitable for extracting a body fluid of a large number of silkworms.

In the method of incising silkworms with a laser,
There is a problem that the useful substance in the body fluid may be denatured by the influence of the heat of the laser.

Further, by any of the above methods,
The body fluid must be squeezed out from the wound of the silkworm, etc., and there is a problem that the working efficiency is poor.

[0007] The present invention has been made in view of such circumstances, and it is possible to continuously extract the body fluid of a large number of silkworms without causing the denaturation of useful substances in the body fluid, and efficiently. It is an object of the present invention to provide a method for extracting bodily fluids of silkworms that can perform bodily fluid extraction.

[0008]

The present invention has the following configuration in order to achieve the above object. That is, the present invention is to inject a high-pressure liquid from a nozzle toward the silkworm, and to make a through-hole in the silkworm by the injected high-pressure liquid to extract the body fluid of the silkworm.

[0009]

The operation of the present invention is as follows. That is,
A jet jet of high-pressure liquid from the nozzle penetrates the body of the silkworm to make a through hole for extracting body fluid. At this time, the jet jet that has been jetted passes through the body of the silkworm at a high speed, and the body fluid of the silkworm is extracted outside the body of the silkworm along with the jet jet due to the accompanying effect at that time.

[0010]

An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a diagram showing a schematic configuration of an apparatus for carrying out the silkworm body fluid extraction method of the present invention. In the drawing, reference numeral 1 denotes a high-pressure water pump. The high-pressure water pump 1 pressurizes water supplied from a water tank (not shown) to a high pressure and supplies the water to a jet nozzle 3 via a valve 2. ,
It switches the supply and cutoff of high-pressure water from the high-pressure water pump 1 to the nozzle 3. Further, the nozzle 3 is configured to be able to spray the supplied high-pressure water in a different direction. A jet jet sprayed from the injection port 3a of the nozzle 3 supplied with high-pressure water toward the silkworm 4, which has grown into a mature silkworm from the age of 4 containing a useful substance in the body fluid, makes a through hole in the silkworm 4, Silkworm 4
The body fluid is extracted. The extracted body fluid is received in the container 5 below the silkworm 4, but in order to prevent the container 5 from being perforated by the jet jet, the power of the jet jet is provided between the silkworm 4 and the container 5. The cushioning material 6 which softens is provided.

In the high-pressure pump 1, a through hole is made in the silkworm 4,
It is preferable to pressurize the supplied water at about 100 kg / cm ² to 4000 kg / cm ² so as to sufficiently obtain the accompanying effect described later. Also, when drilling through holes in silkworm 4,
In order to prevent extraction of unnecessary substances such as the internal organs of the silkworm 4 and to reduce the amount of water penetrating through the body of the silkworm 4, the nozzle of the jet jet should have a diameter of about 0.05 mm to 1.0 mm. It is preferable to adjust the three injection ports 3a. Further, a jet jet is jetted onto the silkworm 4 and the through hole is opened at a position slightly from the tail angle 4a of the silkworm 4 to the head 4b.
Closer positions are preferred. This is because the body fluid can be extracted more by making a through hole in the abdomen 4c of the silkworm 4, and extracting the body fluid more. It will be extracted together. Therefore, in order to extract more body fluid without extracting internal organs and the like, a through hole is formed at a position slightly closer to the head 4b from the above-mentioned tail angle 4a.

Next, a method of extracting bodily fluid from silkworms using the apparatus of the present embodiment will be described. First, the injection port 3a of the nozzle 3 is formed so that a through hole by the jet jet is formed at the above-described predetermined position.
Set silkworm 4 under. Next, the valve 2 is switched to supply high-pressure water from the high-pressure water pump 1 to the nozzle 3,
The bodily fluid of the silkworm 4 is extracted by making a through hole at a predetermined position of the silkworm 4 with a jet jet ejected from the injection port 3 a of the nozzle 3. At this time, the jet jet that has penetrated the silkworm 4 passes through the body of the silkworm 4 at a high speed, and the body fluid of the silkworm 4 is extracted outside the body of the silkworm 4 with the jet jet due to the accompanying effect at that time.
Further, when a through hole is formed by a jet stream, heat generation is small, and there is no danger of the useful substance in the body fluid being denatured by heat. During the extraction of the body fluid, the injection port 3 of the nozzle 3
a may be slightly shifted so that a long through hole is made in the silkworm 4. In this way, more body fluid can be extracted by the accompanying effect. However, in this case, it is necessary to pay attention to the direction and amount of displacement of the injection port 3a of the nozzle 3 so that the internal organs and the like are not extracted together. And
The valve 2 is switched so that the supply of high-pressure water from the high-pressure water pump 1 to the nozzle 3 is shut off. The time for supplying high-pressure water from the high-pressure water pump 1 to the nozzle 3 by switching the valve 2, that is, the time for extracting the bodily fluid of the silkworm 4, is approximately 0.
It is preferably in the range of 05 seconds to 1 second. This is because if it is too short, the amount of body fluid extracted will be small, and if it is too long, internal organs and the like may be extracted together due to the accompanying effect. In addition, if the valve 2 can be automatically switched, it is convenient when the bodily fluid extraction operation of a large number of silkworms 4 is performed continuously, and therefore, for example, an electromagnetic valve or the like is preferably used as the valve 2. Finally, from the body fluid of the silkworm 4 containing water received in the container 5,
A useful substance such as interferon is separated and taken out.

In the above-described embodiment, the through hole is formed in the silkworm 4 by a jet jet pressurized with water.
The present invention is not limited to this. For example, the body fluid may be extracted by pressurizing a chemical for separating a useful substance from the extracted body fluid, injecting it into the silkworm 4, and making a through hole.

[0014]

As is apparent from the above description, according to the present invention, a through hole is made in a silkworm by a jet jet, and a bodily fluid is extracted by an accompanying effect at that time.
Through holes can be formed in the silkworm in a short time, and the work of squeezing out the bodily fluid as in the conventional method is not required, so that the working efficiency of bodily fluid extraction can be improved. In addition, since little heat is generated when the through hole is opened by the jet jet, the useful substance in the body fluid can be prevented from being denatured by heat. In addition, by repeatedly injecting and stopping the injection of high-pressure liquid from the nozzle, the bodily fluids of many silkworms can be extracted continuously, so the risk of disease transmission between silkworms as in the conventional method should be considered. It is possible to mass-produce without mass production.

[Brief description of the drawings]

FIG. 1 is a diagram showing a schematic configuration of an apparatus for performing a bodily fluid extraction method of a silkworm of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... High-pressure water nozzle 2 ... Valve 3 ... Jet nozzle 4 ... Silkworm 5 ... Container 6 ... Buffer material

Claims (1)

(57) [Claims] 1. A method for extracting a bodily fluid of a silkworm, comprising injecting a high-pressure liquid from a nozzle toward the silkworm and extracting a bodily fluid of the silkworm by making a through hole in the silkworm with the injected high-pressure liquid.