JP3277602B2 - Amino acid enriched soy protein composition - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a soybean protein composition enriched in a water-soluble amino acid. Specifically, such a composition can be used as a feed for marine animals.

[0002]

2. Description of the Related Art As a sustained-release composition of a water-soluble amino acid, a composition in which a water-soluble amino acid is coated with a water-insoluble polymer material such as zein or a cellulose membrane or an oil or fat is known. For example, Japanese Unexamined Patent Publication (Kokai) No. 4-173060 discloses a composition for marine feed in which a water-soluble amino acid is coated with a fat or oil so that the content component is not eluted and sustained release and feed efficiency are improved. .

[0003] Further, other compositions for feed for marine animals include those containing iron as a main component of a complex amino acid (see JP-A-59-175851) and liquid eggs of fish as active ingredients. And homogenized protein powders, vitamins, amino acids, soybean phospholipids, water, etc., and then dry-pulverized (see JP-A-1-304852). The latter is particularly useful for shrimp cultivation. Intended for initial formula feed.

[0004] However, although water-insoluble polymers such as cellulose membranes and extremely hardened oils used as coating materials have excellent sustained-release effects, their digestibility is not always clear. In some cases, the active ingredient may pass through the body while being coated, and may not be fully utilized. On the other hand, soy protein itself has high nutritional value and can be used as a protein source by itself, so that an effective composition can be expected to be provided.

[0005]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to carry a water-soluble amino acid which is particularly effective for aquaculture of marine animals in such a state that it is difficult to elute easily in water (providing sustained release). It is to provide an amino acid enriched composition.

[0006]

Means for Solving the Problems The inventors of the present invention have been conducting studies to impart sustained-release properties to water-soluble amino acids.
By treating a certain quality of soybean protein, which has been conventionally used as a binder for various feed compositions, with a certain amount of a water-soluble amino acid under certain conditions, a sustained-release amino acid-enriched composition can be obtained. And arrived at the present invention.

Accordingly, according to the present invention, there is provided an amino acid-enriched composition in which the solubility of a water-soluble amino acid is suppressed,
(A) the water-soluble amino acid is 50% by weight or less based on the total composition weight, (b) the soy protein contains at least 60% by weight of the protein, and (c) the water-soluble amino acid and the soy protein Of the kneaded material in an aqueous medium of 5.0 to 5.0
A composition is provided, which is subjected to gelation by heating under a pH condition of 6.5 followed by drying treatment.

[0008] Such a composition is advantageously used in the application of the feed for aquatic animals provided as another present invention, but its use in other feeds and processed foods is also considered.

[0009]

DETAILED DESCRIPTION OF THE INVENTION The water-soluble amino acids used in the present invention, when included in certain compositions, are readily eluted into water and include, but are not limited to, arginine, lysine, methionine. And the like are typical examples. Of these, arginine is particularly preferred when used as feed for marine animals, especially shrimps.

[0010] As a soybean protein in which these water-soluble amino acids are kneaded in an aqueous medium, it is necessary to use one containing at least 60% by weight of protein. Such a soybean protein is not limited by the production method, but for example, a protein contained in a high-proteinized soybean flour and a non-defatted defatted soybean flour having a protein content of 60% or more as a raw material. A so-called isolated soybean protein prepared by collecting only soybeans can be mentioned.

The water-soluble amino acid and the soybean protein are first kneaded in an aqueous medium. The amount of water used is not particularly limited as long as both components are kneaded sufficiently homogeneously. The mixing ratio of these components is such that the water-soluble amino acid is 50% by weight based on the total amount of the water-soluble amino acid and soybean protein.
It must be: If the amount exceeds 50% by weight, the sustained release of the water-soluble amino acid cannot be expected much.

Next, the kneaded material is adjusted to a pH of 5.0 to 6.5 and then gelled by heating. This pH range is important because the sustained release effect of the water-soluble amino acid is considered to be largely due to gelation of soy protein. Heating is not particularly limited as long as the soybean protein is not significantly denatured, but is generally 70 ° C to 120 ° C, preferably 85 ° C.
C. to 100.degree.

The gelled composition is then dried. Further, depending on the end use, the powder may be pulverized to prepare a powder having a certain particle size. The degree of drying may be such that drying is performed to a water content convenient for pulverization, and is usually about 10%. When used as a feed for marine animals, it is preferable that the feed has a powder particle size in the range of 50 to 1,000 microns. The marine animals referred to in the present invention include, but are not limited to, shrimp, black tiger, hamachi, eel and the like. The composition of the present invention thus obtained can be used as various feeds per se, but may be mixed with other components if necessary.

[0014]

EXAMPLES Hereinafter, the present invention will be described more specifically with reference to examples. Examples 1-7 Isolated soy protein (Adipron-SU, protein content 90%)
80 g, arginine hydrochloride 20 g and water 280 g were mixed, and the pH was adjusted as shown in Table 1 by adding hydrochloric acid. After kneading for 10 minutes, the mixture was packed in a casing and heated at 90 ° C. for 30 minutes. 100-150 ° C after shredding the heated gel into thin chips
Each composition was dried by drying in a dry air current of 10% or less. Each dried composition was pulverized and classified to obtain a sample having an average particle diameter of 200 μm and a 500 μm portion.
And 100 mL of water were placed in a 300 mL Erlenmeyer flask and shaken. After 1 hour, the concentration of arginine in the supernatant was measured by a colorimetric method (8-oxyquinoline bromination method). This result also indicates that the product of the present invention has a lower solubility of water-soluble amino acids than the comparative product.

The elution rate is determined by the following equation, and the results are summarized in Table 1 below. Elution rate = arginine content of supernatant / added arginine content × 100

[0016]

[Table 1]

Example 8 180 g of water was added to 30 g of lysine and 70 g of concentrated soybean protein (Adipron S1: protein content 65%), and the pH was adjusted to 5.5 with sulfuric acid.
After adjusting to 8, a mixed paste was obtained. It was packed in a casing tube, heated at 110 ° C. for 10 minutes, dried in the same manner as in the above example, and pulverized to an average particle size of 300 μm.
The elution rate of this product in an aqueous solution after 1 hour was 72% of that of untreated lysine. Lysine was quantified using an amino acid analyzer. Arginine strengthening soy protein (pH5.6 the prototype in Application Example Example 3,
Average particle size 300 microns) with an arginine content of 0.5%
A breeding test of black tiger was carried out with the diet added to increase the weight, and the change in weight gain was examined. For comparison, the case where crystalline arginine was added as it was and the case where arginine coated with cellulose acetate phthalate (CAP) were evaluated at the same time. The results are shown in Table 2 below. The composition of the control diet is as follows. Food composition (control) Fish meal 530 Ika meal 80 Shrimp meal 100 Soybean meal 20 Flour 220 Fish oil 10 Minerals 35 Premix 5 Production method Add a small amount of water, mix well the whole amount, make pasta through meat grinder, steam sterilize and dry. I do.

[0018]

[Table 2]

Arginine is contained in the samples in the respective test plots in the following amounts. Test group 1: The control contains 2.0% by weight of arginine as arginine in the composition protein. Test group 2: control + crystalline arginine (arginine corresponding to 0.5% by weight) was added. Test group 3: Control + sample of Example 3 (arginine corresponding to 0.5% by weight) added. Test group 4: control + CAP-coated arginine (0.
(Arginine equivalent to 5% by weight).

[0020]

According to the present invention, the solubility of the water-soluble amino acid is suppressed (50 to 70 per hour as compared with the untreated amino acid).
%) An amino acid-enriched soy protein composition is provided. Such compositions are particularly useful as feed ingredients for aquatic animals used in water.

Claims (2)

(57) [Claims] 1. An amino acid-enriched soybean protein composition in which the solubility of a water-soluble amino acid is suppressed, wherein (a) the water-soluble amino acid is 50% by weight or less based on the total weight of the composition, and (b)
(B) heating the kneaded mixture of the water-soluble amino acid and the soy protein in an aqueous medium under a pH condition of 5.0 to 6.5; A composition characterized by being subjected to a drying treatment after gelation. 2. A feed for a marine animal comprising the composition according to claim 1.