JP3239203B2 - Sample stirring device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a sample stirring device for stirring a liquid sample in a container, and more particularly, to homogenizing a liquid sample stored in a container in, for example, an automatic analyzer. The present invention relates to a sample stirring device for stirring for a purpose.

[0002]

2. Description of the Related Art Conventionally, as a sample stirring device for stirring a liquid sample such as urine or blood, an operation of sucking a fixed amount of a sample in a container and discharging the sample in the container again is repeated several times. There has been known a device configured to perform suction and discharge agitation to make the distribution state of the formed components in a sample uniform.

[0003] Such a sample agitating device is composed of a pipette vertically arranged and aspirating and discharging a sample, and a spiral tube connected to the upper end of the pipette and agitating the aspirated sample. A pump for sucking and discharging the sample connected to the spiral tube, and a washing line connected to the spiral tube in parallel with the pump. The pipette and the spiral tube have the same inner diameter.

In such a sample stirring device, a sample is sucked from a container by a pipette by a suction operation of a pump to flow into a helical tube to perform stirring, and then the inside of the spiral tube and the inside of the pipette are discharged by a discharge operation of the pump. The sample is discharged into the same container again.

The sample is stirred by repeating such suction and discharge several times. When the stirring is completed, the outer and inner surfaces of the pipette and the spiral tube are washed. At this time, the line connecting the helical tube and the pump is also used for cleaning because there is a possibility that the line is contaminated by the sample.

[0006]

In such a sample stirring apparatus, the pipette and the helical tube may have the same inner diameter, and the sample may not be sufficiently stirred. was there.

In addition, it is necessary to clean the line connecting the spiral tube and the pump, and since the total internal extension length of the spiral tube is relatively long with respect to the length of the pipette, the cleaning fluid (the cleaning liquid and / or Alternatively, a large amount of cleaning air is required, and the cleaning time is long.

The present invention has been made in view of such circumstances, and has as its object to provide a sample capable of sufficiently stirring the sample, reducing the amount of cleaning fluid and shortening the cleaning time. It is to provide a stirring device.

[0009]

According to the present invention, a pipette for sucking and discharging a liquid sample placed in a container and a pipette having an inner space with a larger diameter than the pipette and connected above the pipette are provided. The main chamber, which is provided in a shape, and into which the sample sucked by the pipette flows and is stirred, the sub-chamber provided near the main chamber, and the upper part of the main chamber and the upper part of the sub-chamber communicate with each other. A connected connection part, a suction / discharge pump connected to the upper part of the sub-chamber and providing a driving force to the pipette for suction and discharge, and a supply part for supplying a cleaning fluid to the upper part of the main chamber and supplying the cleaning fluid to the main chamber. A cleaning fluid supply line, a first waste liquid line for collecting the cleaning waste liquid from the pipette, and a second waste liquid line connected to the lower part of the sub-chamber for collecting the cleaning waste liquid from the sub-chamber. Sample stirring device is provided comprising a waste line.

As a container for accommodating the liquid sample to be agitated, a container having various sizes, shapes and materials is appropriately selected and used. As one example, there is a so-called tapered test tube in which urine, blood, or the like as a sample is stored in about 5 to 10 milliliters.

The pipette sucks a fixed amount of the sample put in the container and discharges the sample again into the container in a discharge state. Thereby, the sample in the container is once drawn from the pipette into the main chamber and then discharged to the bottom of the container and spreads along the bottom or side wall of the container. Such an operation is repeated several times, and the suction and discharge agitation by the pipette ends. For example, a diaphragm pump is used as a suction / discharge pump that applies a driving force for suction and discharge to the pipette.

The main chamber has an inner space with a larger diameter than the pipette and is provided above the pipette in a connected manner.
The sample sucked by the pipette flows into the main chamber. At this time, since the main chamber has a larger diameter than the pipette, the inflowing sample spreads in the main chamber, and the stirring is sufficiently performed.

The sub-chamber is provided in the vicinity of the main chamber, that is, above or beside the main chamber, and the upper part is connected to the upper part of the main chamber via a connection part. Further, a suction / discharge pump for applying a driving force to the pipette for suction / discharge is connected to the upper portion of the sub-chamber. In other words, the sub-chamber is provided between the main chamber and the pump. The sample may flow into the sub-chamber from the main chamber due to the suction and discharge operation of the suction and discharge pump.

As the connecting portion, for example, there is a connecting tube (including a rigid body and a flexible tube) or a connecting tube portion provided separately or integrally with the main chamber and the sub-chamber. The inner diameter of the connection is not particularly limited,
It is preferably smaller than the upper inner diameter of the main chamber or the upper inner diameter of the sub-chamber, and more preferably smaller than both the upper inner diameter of the main chamber and the upper inner diameter of the sub-chamber.

The cleaning fluid supply line is connected to the upper part of the main chamber, and supplies the cleaning fluid to the main chamber after the completion of the sample stirring. Here, the cleaning fluid is a cleaning liquid and / or a cleaning fluid.
Or cleaning air. Most of the cleaning fluid supplied to the main chamber from this line flows down from the main chamber into the pipette, but partly flows into the sub-chamber.

The first waste liquid line is for introducing and collecting the cleaning waste liquid after cleaning the inner and outer surfaces of the pipette to a waste liquid chamber or the like. This first waste liquid line is usually provided between the cleaning spits for cleaning the pipette and the waste liquid chamber.

The second waste liquid line is for introducing and collecting the sample and / or the cleaning fluid flowing from the main chamber to the sub-chamber to the waste liquid chamber or the like.

The shapes of the main chamber and the sub-chamber, particularly the shape of the internal space, are not particularly limited. However, it is preferable that each of the upper inner wall has a substantially circular or substantially elliptical horizontal sectional shape.

When the main chamber has a substantially circular or substantially elliptical horizontal cross-sectional shape on its upper inner wall, the cleaning fluid supply line is connected to the upper inner wall of the main chamber in a substantially horizontal tangential manner. Is preferred. When such a main chamber and the cleaning fluid supply line are used, the cleaning fluid supplied from the cleaning fluid supply line is introduced along the upper inner wall of the main chamber, and flows down while spirally cleaning the inner wall. After that, the pipette enters the inside of the pipette and flows down while washing its inner surface. Therefore, the main chamber and the inner surface of the pipette can be efficiently and reliably cleaned without supplying extra cleaning fluid into the main chamber.

[0020]

Embodiments of the present invention will be described below with reference to the drawings. Note that the present invention is not limited by this.

FIG. 1 is an explanatory view of the configuration of a sample stirring / suction device D according to one embodiment of the present invention. In FIG. 1, a sample stirring / suction device D includes a first pipette 1, a second pipette 2, a stirring chamber 3, a diaphragm pump 4 as a suction / discharge pump, a sampling valve 5, a syringe pump 6, a cleaning fluid supply line. 7, a cleaning spits 9, a waste liquid chamber 10, a first waste liquid line 11, a second waste liquid line 12, and a control unit (not shown).

Reference numeral 13 denotes a tapered test tube as a sample container. This test tube 13 contains about 4.0 ml to a maximum of 10.0 ml of raw urine as a liquid sample.

The first pipette 1 is made of stainless steel and is used for sucking and discharging the raw urine placed in the test tube 13. The second pipette 2 is made of stainless steel, and is for sucking and collecting raw urine from the test tube 13 after the end of the suction and discharge agitation by the first pipette 1. FIG.
3, the first pipette 1 has a large diameter (2.0 mm in inner diameter), and the second pipette 2 has a small diameter (0.7 mm in inner diameter). Further, as shown in an enlarged manner in FIG. 4A, the two pipettes 1 and 2 are integrally joined to each other at the outer walls by bonding with a thermoplastic resin. Reference numeral 14 in FIG. 4A indicates the joint. Note that, instead of the pipettes 1 and 2 in FIG.
Pipettes 1 and 2 by resin molding as shown in (b)
(Cylindrical outer shape) can also be used.

The stirring chamber 3 includes a main chamber 15 connected above the first pipette 1 and a main chamber 1.
5 and a sub-chamber 16 provided beside.

The inner space of the main chamber 15 has a circular horizontal cross-sectional shape on the upper inner wall, the lower inner wall is tapered, and the lower end is the first pipette 1.
Is connected to the upper end. Since the main chamber 15 has such an internal space, the raw urine sucked by the first pipette 1 flows into the main chamber 15 and spreads, and is sufficiently stirred.

The internal space of the sub chamber 16 is also the main chamber 1
5 is configured in the same manner. The inner space of the sub-chamber 16 is configured such that its upper end is flush with that of the main chamber 15. The volume of the sub-chamber 16 is about one fifth of that of the main chamber 15.

The upper end of the main chamber 15 and the sub-chamber 16
Are connected to each other in a communication manner by a U-shaped connection pipe 17 as a connection part. In addition, one connection port 15 a is provided in the upper end side wall of the main chamber 15. The connection port 15 a is configured such that its axis is horizontally tangent to the inner wall at the upper end of the main chamber 15.

The pumping operation of the pump 4 causes the first pipette 1 to suck and discharge raw urine.

The sampling valve 5 is connected to the second pipette 2
Quantitatively collect the raw urine aspirated by. The pump 6 is driven by a motor 18, and the suction operation of the pump 6 causes suction by the second pipette 2.

The cleaning fluid supply line 7 is connected to the main chamber 1
5 connection port 15a. The fluid supply line 7 is provided with a T-shaped branch portion 8, and a predetermined ratio of a pressurized cleaning liquid (0.5 kg / cm ² ) and pressurized air (0.5 kg / cm ² ) as a cleaning fluid. The mixture or one of the fluids can be supplied to the main chamber 15. The pressurized cleaning liquid is introduced from the first supply line 19, and the pressurized air is introduced from the second supply line 20, respectively.
The fluid supply line 7 between the branch portion 8 and the connection port 15 a of the main chamber 15 has a cleaning liquid line opening / closing valve 3.
0a and a pressurized air line opening / closing valve 30b are provided.

The cleaning spits 9 are used when cleaning the inner and outer surfaces of the first pipette 1 and the second pipette 2. The spits 9 include a waste liquid receiving unit 21 that receives the waste liquid generated by the cleaning, a cleaning unit 22 provided continuously above the waste liquid receiving unit 21, and a waste liquid receiving unit 2 on the side of the waste liquid receiving unit 21.
1 and an air discharge portion 23 provided in communication with the air discharge device 1. One cleaning liquid inlet 22 a is provided horizontally above the cleaning unit 22. The introduction port 22 a is configured so that its axis is oriented in a tangential direction of the cleaning unit 22. From the inlet 22a, pressurized cleaning liquid (0.5 kg
/ Cm ² ) is introduced into the cleaning unit 22. At the lower portion of the inner wall of the cleaning section 22, an air suction port 22b is branched into eight and provided in a horizontal radial manner. Air suction port 22b is 400m
It is connected to a vacuum pump (not shown) of mHg.

The waste liquid chamber 10 has a valve 2 for opening to the atmosphere.
4 are provided. The waste liquid chamber 10 is connected to a waste liquid receiving part 21 of the spitz 9 by a first waste liquid line 11.

One connection port 25 is provided horizontally on the upper side wall of the sub-chamber 16. The connection port 25 is connected to the pump 4 via a suction / discharge stirring line 26.
The suction / discharge stirring line 26 is provided with a line opening / closing valve 27.

One connection port 28 is provided horizontally on the lower side wall of the sub-chamber 16. The connection port 28 is connected to the waste liquid chamber 10 via the second waste liquid line 12. The second waste liquid line 12 has a line opening / closing valve 2.
9 are provided.

Next, various operations of the sample stirring / suction device D will be described with reference to a timing chart of FIG. Various operations of the sample stirring / suction device D are all controlled by the control unit. The numbers on the horizontal axis in the timing chart of FIG.
Represents the number of seconds since the start of the operation of. For example, 165
Means 16.5 seconds after the start of the operation.

With the start of the operation of the sample stirring / suction device D, the first pipette 1 and the second pipette 2 located at the initial positions above and outside the test tube 13 descend at a constant speed, and enter the inside of the test tube 13. Can be put in. After 3.0 seconds, the descent stops, the pump 4 performs the suction and discharge operation, and the first pipette 1 performs suction and discharge of the urine five times every 1.0 second.

That is, the control unit opens the valve 27, closes the valve 29, closes the valve 30, and causes the pump 4 to perform the "suction" operation. This allows
The first pipette 1 performs a suction operation, and 2.0 ml of raw urine is sucked up from the test tube 13 and flows into the main chamber 15 to spread. At this time, the liquid level of the raw urine is
It comes at about one-half the depth of 5. The raw urine that has flowed into the main chamber 15 is sufficiently stirred by spreading in the main chamber 15. Due to the suction operation of the first pipette 1, the raw urine (usually in the form of a water droplet) that has flowed into the sub-chamber 16 via the connection pipe 17 with excess force accumulates at the bottom of the sub-chamber 16. The original urine collected in the sub-chamber 16 in a certain amount is guided to the waste liquid chamber 10 by opening the valve 29 and the valve 24.

After the suction operation by the first pipette 1, the control unit causes the pump 4 to perform the "discharge" operation. As a result, the first pipette 1 performs an ejection operation, and the main chamber 15
The raw urine inside is discharged to the bottom of the test tube 13, spreads along the bottom wall or side wall of the test tube 13, and is sufficiently stirred inside the test tube 13.

The above suction and discharge operations are repeated five times, and the suction and discharge agitation by the first pipette 1 ends (when 13.0 seconds have elapsed from the start of operation).

When the suction and discharge agitation by the first pipette 1 is completed, after 1.0 second, the suction operation of the pump 6 is performed and the second operation is performed.
The test tube 1 takes 2.0 seconds due to the suction operation of the pipette 2.
0.8 ml of raw urine is sucked up from near the bottom of 3 (first stage suction). 0.5 seconds later, the first pipette 1
And the second pipette 2 starts rising at a constant speed. 1.0 second after the start of the ascent, the suction operation of the second pipette 2 is performed again. That is, only the air is reduced to about 0.
Eight milliliters are sucked (second-stage suction), and the raw urine obtained by the first-stage suction is guided further into the sample stirring / suction device D with this air. During the second-stage suction, the first pipette 1 and the second pipette 2 continue to rise.

The raw urine introduced into the sampling valve 5 by such two-stage suction is subjected to quantitative determination.

The first pipette 1 and the second pipette 2 which have continued to ascend stop 3.0 seconds after the start of the ascent, and
Return to the initial position (when 19.5 seconds have passed since the start of operation).
Next, the first pipette 1 and the second pipette 2 are moved backward from the initial position, and are positioned above the washing spits 9 provided in the analyzer.

Thereafter, the first pipette 1 and the second pipette 2 start descending into the spitz 9 after a lapse of 22.0 seconds from the start of operation. Then, over 2.0 seconds, the outer surfaces of the lower halves are cleaned. That is, the lower half of the first and second pipettes 1 and 2 is
The pressure cleaning liquid is introduced into the cleaning unit 22 from the introduction port 22a of the cleaning unit 22 in a discharge manner while descending from the cleaning liquid to the waste liquid receiving unit 21. Thereby, the outer surfaces of the lower halves of the first pipette 1 and the second pipette 2 are cleaned.

During this time, the first pipette 1 and the second pipette 2 continue to descend in the spits 9. And the first
At the same time as the cleaning of the outer surfaces of the lower halves of the pipette 1 and the second pipette 2 is completed (after a lapse of 24.0 seconds from the start of operation), the cleaning of the main chamber 15, the sub-chamber 16 and the inside of the first pipette 1 is performed. Will be

That is, the control unit closes the valve 27, closes the valve 29, and opens the valve 30. The control unit supplies the mixture of the pressurized cleaning liquid and the pressurized air to the fluid supply line 7. Feed for 2.0 seconds. Then, the mixture is introduced into the main chamber 15 from the connection port 15a along the inner wall at the upper end of the main chamber 15 (until 26.0 seconds have elapsed from the start of operation). Then, after flowing down while spirally cleaning the inner wall, it enters the inside of the first pipette 1 and flows down while cleaning its inner surface. The lowering of the first pipette 1 and the second pipette 2 in the Spitz 9 stops after a lapse of 26.0 seconds from the start of operation.

Next, the control unit opens the valve 29 and supplies the mixture to the fluid supply line 7 for 2.0 seconds. Then, the mixture is mostly connected to the connection port 15a.
Is introduced into the main chamber 15 along the inner wall at the upper end of the main chamber 15, and a part thereof is introduced from the connecting pipe 17 into the sub-chamber 16. Main chamber 15
The mixture introduced into the main chamber 15
And the inner surface of the first pipette 1 are cleaned. The mixture introduced into the sub-chamber 16 collects in the sub-chamber 16 after cleaning its inner wall,
Led to.

The waste liquid generated by washing the inside of the main chamber 15 and the first pipette 1 is discharged to the waste liquid receiving section 2 of the Spitz 9.
Stored in 1.

The main chamber 1 made of the mixture as described above
5. When the cleaning of the inside of the sub-chamber 16 and the first pipette 1 is completed, 8.8.0 seconds have elapsed from the start of the operation and 8.
During 0 seconds, removal of the liquid droplets left inside these is performed.

That is, the valve 27 is closed and the valve 2
When the valve 9 is open and the valve 30 is open, the pressurized air is supplied to the fluid supply line 7 for 2.0 seconds. Next, with the valve 27 closed, the valve 29 closed, and the valve 30 open, pressurized air is supplied to the fluid supply line 7 for 6.0 seconds. With the above operation, the liquid remaining on the inner walls of the main chamber 15 and the sub-chamber 16 and the inner surface of the first pipette 1 is blown off by the pressurized air (air blow), and the waste liquid chamber 10 and the waste liquid in the spits 9 are received. To the section 21.

Next, the cleaning of the inner surface of the second pipette 2 will be described. That is, the control unit operates the cleaning mechanism (not shown) to introduce the pressurized cleaning liquid into the second pipette 2. As a result, 26.
Cleaning of the inner surface of the second pipette 2 is performed for 3.5 seconds from the lapse of 0 seconds.

At the elapse of 30.0 seconds from the start of the operation, the cleaning of the outer surfaces of the first pipette 1 and the second pipette 2 is started. At this point, the first pipette 1 and the second pipette 2
Is stopped at the lowest position in Spitz 9. That is,
The lower half of the first pipette 1 and the second pipette 2 is located in the waste liquid receiving part 21 of the spits 9, and the lower part of the upper half is the washing part 22.
It is in.

In this state, the pressurized cleaning liquid is introduced into the cleaning unit 22 from the inlet 22a of the cleaning unit 22 in a discharge state for 2.0 seconds. As a result, the lower outer surfaces of the upper half of the first pipette 1 and the second pipette 2 are cleaned (32.0 seconds have elapsed since the start of operation). Then, immediately, pipette 1
And the second pipette 2 rises, and at a certain position in the spitz 9, slows down and rises while maintaining the reduced speed. Next, the speed is increased at a certain position in the spitz 9 and the ascent is increased while maintaining the increased speed. When the elapse of 37.0 seconds from the start of the operation, the ascent is stopped. at this point,
The first pipette 1 and the second pipette 2 are located above the outside of the spitz 9.

In parallel with such an operation, the first after cleaning is performed.
The water-drop-like liquid left on the outer surfaces of the pipette 1 and the second pipette 2 is removed. That is, the control unit operates the vacuum pump to suck air from the air suction port 22b of the washing unit 22 so that the liquid remaining on the outer surfaces of the first pipette 1 and the second pipette 2 is discharged to the waste liquid receiving unit. Fall to 21.

In the sample agitating / suctioning device D, the raw urine put in the test tube 13 is moved by both the main chamber 15 and the test tube 13 by the suction and discharge operation of the first pipette 1 as described above. Stir well.

In the sample agitating / suctioning device D, the main chamber 15 and the fluid supply line 7 are provided instead of the spiral tube in the conventional device. Is supplied along the upper inner wall of the main chamber 15 and flows down while spirally cleaning the inner wall.
And flows down while cleaning the inner surface. Therefore, the main chamber 15 and the inner surface of the first pipette 1 can be efficiently and reliably cleaned without supplying an extra cleaning fluid into the main chamber 1, and the amount of the cleaning fluid can be reduced and the cleaning time can be reduced. Can be achieved.

Further, in the sample agitating / suctioning apparatus D, the sub chamber 1 as described above is provided beside the main chamber 15.
6 is provided, even if the raw urine in the main chamber 15 flows into the sub-chamber 16 via the connecting pipe 17 during the suction operation of the first pipette 1, the raw urine is
And does not flow into the pump 4 through the suction / discharge stirring line 26. Therefore, it is not necessary to clean the suction / discharge agitation line 26 and the pump 4, and from this point, the amount of the cleaning fluid can be reduced and the cleaning time can be shortened.

[0057]

The sample stirring device according to the present invention is configured as described above, and has the following remarkable effects.

That is, in the sample stirring apparatus according to the first aspect, the pipette for sucking and discharging the liquid sample and the internal space having a larger diameter than the pipette are provided in a connected state above the pipette. A main chamber for sample agitation, a subchamber provided near the main chamber, a connection part connected so that an upper part of the main chamber and an upper part of the subchamber communicate with each other, and a connection part connected to an upper part of the subchamber. A sample suction / discharge pump, a cleaning fluid supply line connected to the upper part of the main chamber, a first waste liquid line for collecting cleaning waste liquid from the pipette, and a cleaning connected to the lower part of the sub chamber and from the sub chamber. A second waste liquid line for collecting the waste liquid. Therefore, the sample placed in the container is sufficiently stirred in both the main chamber and the container by the suction and discharge operation of the pipette.

The cleaning fluid supplied from the fluid supply line is introduced into the upper portion of the main chamber, flows down while cleaning the inside, and then flows into the pipette while cleaning the inner surface thereof. Therefore, the chamber and the inner surface of the pipette can be efficiently and reliably cleaned without supplying an extra cleaning fluid into the main chamber, and the amount of the cleaning fluid can be reduced and the cleaning time can be reduced. .

Further, a sub-chamber is provided in the vicinity of the main chamber, and both chambers are connected to each other by a connecting portion between the upper portions, so that the sample in the main chamber passes through the connecting pipe during the suction operation of the pipette 1. Even when the sample flows into the sub-chamber, the sample only accumulates in the sub-chamber, and does not flow into the sample suction / discharge pump via the suction / discharge stirring line. Therefore, it is not necessary to clean the suction / discharge stirring line and the pump, and from this point of view, it is possible to reduce the amount of the cleaning fluid and shorten the cleaning time.

In the sample stirring device according to the second aspect,
The main chamber and the sub-chamber are arranged in a horizontal position, and the connection part comprises a connecting pipe having an inner diameter smaller than any of the upper inner diameter of the main chamber and the upper inner diameter of the sub-chamber. Therefore, in addition to the above-described effects achieved by the sample stirring device according to claim 1, it is possible to reduce the amount of cleaning fluid and shorten the cleaning time by eliminating the need to clean the suction / discharge stirring line and the pump. The above effects can be more reliably achieved.

In the sample stirring device according to the third aspect,
The main chamber has a substantially circular or substantially elliptical horizontal cross-sectional shape on the upper inner wall thereof, and the cleaning fluid supply line is connected to the upper inner wall of the main chamber in a substantially horizontal tangential manner. The cleaning fluid supplied from the line is introduced along the upper inner wall of the main chamber, flows down while spirally cleaning the inner wall, and then flows inside the pipette while cleaning the inner surface thereof. Therefore,
In addition to the above-mentioned effects achieved by the sample stirring device according to claim 1 or 2, the main chamber and the inner surface of the pipette can be efficiently and reliably cleaned without supplying an extra cleaning fluid into the main chamber. The above effects can be more reliably achieved.

[Brief description of the drawings]

FIG. 1 is an explanatory view of the overall configuration of a sample stirring / suctioning apparatus according to one embodiment of the present invention.

FIG. 2 is an enlarged configuration explanatory view of a main part of the sample stirring / suction device of FIG. 1;

FIG. 3 is an enlarged vertical sectional view of lower end portions of a first pipette and a second pipette in the sample stirring / suction device of FIG. 1;

FIG. 4 is an enlarged bottom view of lower end portions of a first pipette and a second pipette in FIG. 3;

FIG. 5 is a timing chart illustrating various operations in the sample stirring / suction device of FIG. 1;

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 1st pipette (pipette) 2 2nd pipette 3 Stirring chamber 4 Diaphragm type pump (pump for suction and discharge) 5 Sampling valve 6 Syringe type pump 7 Fluid supply line for cleaning 8 T-shaped branch part 9 Spitz for cleaning 10 Waste liquid chamber 11th Reference Signs List 1 waste liquid line 12 second waste liquid line 13 test tube (container) 14 joint 15 main chamber 16 sub-chamber 17 connection pipe (connection) 18 motor 19 first supply line 20 second supply line 21 waste liquid receiver 22 washing unit 23 Air discharge unit 24 Valve 25 Connection port 26 Suction / discharge stirring line 27 Valve 28 Connection port 29 Valve 30 Valve

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Claims (3)

(57) [Claims] 1. A pipette for aspirating and discharging a liquid sample placed in a container, and having a larger internal space than the pipette, provided in a connected state above the pipette, and aspirated by the pipette. A main chamber into which the sample flows and is agitated; a sub-chamber provided near the main chamber; a connection portion connected so that an upper portion of the main chamber and an upper portion of the sub-chamber communicate with each other; A suction / discharge pump connected to the upper portion and providing a driving force to the pipette for suction / discharge; a cleaning fluid supply line connected to the upper portion of the main chamber and supplying a cleaning fluid to the main chamber; And a second waste liquid line connected to the lower part of the sub-chamber and for collecting the cleaning waste liquid from the sub-chamber. Fee stirring device. 2. The method according to claim 1, wherein the main chamber and the sub-chamber are arranged in a lateral position, and the connecting portion comprises a connecting pipe having an inner diameter smaller than both the upper inner diameter of the main chamber and the upper inner diameter of the sub-chamber. The sample agitator according to the above. 3. The main chamber has a substantially circular or substantially elliptical horizontal sectional shape on an upper inner wall thereof, and a cleaning fluid supply line is connected to the upper inner wall of the main chamber in a substantially horizontal tangential manner. Item 3. The sample stirring device according to Item 1 or 2.