JP3236751U - Optical system for detecting fluorescence polarization and degree of polarization measurement unit - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an optical system which can be applied to detect fluorescent polarization of a sample in the field and can easily and accurately measure fluorescence polarization. In an optical system for detecting fluorescent polarization, a polarized beam emitting unit 10 is composed of a semiconductor laser device 101, a collimating lens 102, an optical filter 103, and a polarizing plate 104. The semiconductor laser device 101 emits a polarized laser beam having a specified wavelength. The collimating lens 102 collimates the laser beam. After being collimated, the laser beam passes through the optical filter 103 and the polarizing plate 104. The sample is located in the square tank 2a. The polarization degree measuring unit consists of a pair of detector units. Each detector unit includes a photoelectric detector 31, a collimating lens 32, and a polarizing plate 33. [Selection diagram] FIG. 4

Description

The present invention relates to the technical field of optics, and particularly to an optical system for detecting fluorescent polarization and a degree of polarization measuring unit.

Measurements of the degree of fluorescence polarization (FP) are based on changes in the moment of inertia in the target molecule. In order to induce a change in the moment of inertia, the sample is mixed with a probe having a complementary sequence of target DNA. The probe also has a FAM-fluorescent label attached to the 5'position. During hybridization, the DNA carrying the FAM-probe becomes heavier and longer, resulting in a change in the moment of inertia. When the dipole moment of the FAM-probe and the incident laser beam are in the same polarization direction, the FAM-probe absorbs photons of a specific wavelength (483 nm). Also, due to its excitation lifetime, the FAM-probe emits fluorescent photons within an accurate time (2 nsec). Such fluorescence occurs in any FAM-probe excited by the incident of a laser beam, whether or not it is attached to the DNA. However, since the FAM-probe attached to DNA has a heavy total mass, the moment of inertia is larger than that of the FAM-probe not attached. That is, the FAM-probe attached to DNA rotates much slower than the FAM-probe not attached. Therefore, when fluorescence is generated within the emission time (2 nsec), the emitted fluorescent photons exhibit two different polarization characteristics, that is, they are attached to or not attached to DNA. The Stokes vector clearly defines a polarization property called the degree of polarization.

According to the measurement result of a typical degree of polarization, the molecular weight of the unattached FAM-probe is about 5000 g / mol, the medium-sized DNA is about 10,000 g / mol, and the large-sized DNA is about 20000 g / mol.

Therefore, FP measurement can be applied to the identification / selection of the target DNA sequence as long as the weight changes in the probe-DNA hybridization action.

FP measurement is a simple and accurate DNA testing method. Unlike other DNA testing methods, FP measurements have the advantage that in most cases pre-measurement filtration is not required. In recent years, FP has become one of the leading tools for analyzing hybridization phenomena in the field of biology, and users can observe such phenomena in real time by a simple and direct measurement process of FP measurement. be. In addition, FP measurements have the tremendous advantage that, in most cases, pre-measurement filtration is not required. Currently, in cases such as pandemics and food poisoning, the need for DNA testing in the "field" is extremely increasing. In such cases, on-site screening is a very important procedure. On-site screening requires easy sample preparation, portable equipment, battery operation, and quick and accurate measurement results. Therefore, FP measurement can be one of the most promising analytical methods for on-site measurement. However, all the conventional FP measurement systems are huge and expensive, and it is impossible to apply these devices to the measurement in the field. Therefore, if we can provide a battery-operated and portable FP measuring device, it will make a great contribution to society.

The present invention provides an optical system that detects fluorescent polarization easily and accurately. The optical system is applicable for detecting fluorescent polarization of a sample in the field. The optical system for detecting fluorescent polarization in the present invention uses a laser light source, a simple optical structure, a conventional photoelectric unit, a shortened optical path length, and a plurality of light sources capable of selecting an incident wavelength. Also, the optical system for detecting fluorescent polarization in one embodiment of the present invention further includes a battery operating system and a network connection. By these means, the optical system for detecting the fluorescent polarization of the present invention can achieve the purpose of the point of care.

The optical system for detecting fluorescent polarization provided in the embodiment of the present invention can achieve the size of a portable device, the accuracy and reproducibility of FP measurement by simplifying the laser optical system. In addition, it consumes less power, can be supplied with electricity by a battery, and can prepare a sample quickly and easily, which is advantageous for on-site measurement.

In the present invention, the above object is a system using a semiconductor laser diode or an LED as a light source, a system using only one kind of lens unit, that is, a collimating lens as a light source, and a horizontal and vertical polarized beam using a pair of splitters. A system that detects each, a system that detects horizontal and vertical polarized beams using a polarized beam splitter, a system that detects polarization using a pair of silicon photoelectric detectors, and a silicon photoelectric detector that is directly mounted on the polarized beam splitter. A system to be used, a system using multiple light sources, a system in which a collimated laser beam is directly incident on a sample, a system in which the detection angle by the fluorescent beam can be adjusted, a system in which the fluorescence intensity can be measured at the same time, and a system in which light absorption is measured at the same time. It is realized by a possible system.

To achieve the purpose of mobile device size, a laser diode is used as the light source instead of the usual halogen lamp / tungsten bulb / HID lamp. A good monochromatic light source is required for the measurement of fluorescence polarization. This is because fluorescent agents such as FITC, which are commonly used for measuring fluorescent polarization, have a very narrow absorption bandwidth (493 nm ± 10 nm). Therefore, a 493 nm monochromatic light source is required to excite the FITC material. In the conventional design, the system uses a halogen lamp, a tungsten bulb or a HID lamp as a light source. 1A and 1B show emission spectra from halogen lamps and HID lamps. As shown, halogen lamps and HID lamps have a wide spectrum. Therefore, when a specific wavelength is selected using a monochromator or a bandpass filter, the intensity of the selected wavelength becomes very weak (FIG. 1C). On the other hand, in the case of a semiconductor laser diode, the emitted light is substantially monochromatic light (FIG. 2). Therefore, the relative intensity of the selected wavelength is significantly stronger than the relative intensity of the halogen lamp or the HID lamp. Therefore, when a semiconductor laser diode is used as a light source of an optical system for detecting fluorescence polarization, the emitted fluorescence becomes stronger. Further, when a laser diode is used as a light source, a considerably complicated / expensive monochromator can be omitted from the system.

The optical system of the present invention shortens the optical path and omits the collimating lens by detecting the polarization by the pinhole and the polarization beam splitter. FIG. 3 shows an optical system for detecting typical fluorescent polarization. The incident light is monochromated by a monochromator, and then collimated and polarized by a polarizing plate. The polarized beam is split into two by a splitter. Half of the beam is transmitted to the photomultiplier tube via the collimating lens. The other half of the beam is sent to the sample and undergoes a fluorescence reaction. The emitted fluorescent photon passes through another polarizing plate that obtains the maximum output by rotation, and then reaches the photoelectric detector via another collimating lens. FIG. 7 shows the structure of the optical system according to the present invention including the fluorescence polarization detection unit of the present invention. The laser beam is collimated and sent directly to the sample. The fluorescence polarization detection unit consists of a pinhole, a polarization beam splitter and two silicon photoelectric detectors. In the system, a polarizing plate, a splitter having a collimating lens, and a rotating polarizing plate can be omitted. According to the optical system of the present invention, shorter optical paths and less glass surface are provided. The intensity of light decreases with the reciprocal of the square of the distance. Further, by omitting the collimating lens, the reflection loss caused by each glass surface can be reduced. Also, since the silicon photoelectric detector is mounted directly on the polarization beam splitter and provides a shorter optical path length, the collimating lens is omitted and the optical calibration of the photoelectric detector is not required. Further, in another design of the optical system of the present invention, a plurality of light sources are used to select the incident beam. As shown in FIG. 8, by designing without the monochrome meter and the optical unit, the optical system of the present invention can use a plurality of laser light sources or LED light sources including a polarizing plate. The system can also measure different fluorescent labels with different excitation wavelengths with the same detection unit.

The optical system of the present invention uses a low-cost silicon photoelectric detector without a photomultiplier tube. In a typical fluorescence polarization measurement system, a photomultiplier tube is used to detect fluorescence photons. This is because the intensity of the excitation beam is weak, so that the emitted fluorescent photons are also very weak. However, the optical system of the present invention uses a strong monochromatic light from a laser diode. As a result, relatively strong fluorescence is emitted, so that even a low-cost silicon photoelectric detector can measure fluorescence polarization. In the present invention, by using a laser light source and an extremely short optical path, it is possible to detect polarization by a pair of low-cost silicon photoelectric detectors, whereas in most fluorescent polarization measurement systems, expensive optoelectronic multiplication is performed. The use of vessels is mandatory.

In the optical system of the present invention, the monochromatic wave sorting unit is omitted. Monochromators are extremely sensitive to changes in temperature and vibrations in the environment. Therefore, the current optical system for detecting fluorescent polarization is not suitable for use in the "point of care". On the other hand, the optical system using the laser light source in the present invention does not require the use of a monochromator, so that the cost and size of the system can be reduced.

In the optical system of the present invention, since it is directly excited by a laser beam, a waveguide unit such as an optical fiber is omitted. Due to the size of the monochromator, it is difficult to make the beam directly incident on the sample surface in the case of a system equipped with a monochromator. Therefore, an optical fiber must be used to guide the incident beam to the surface of the sample. In addition, the optical fiber collects the emitted fluorescent photons and guides them to the photomultiplier. On the other hand, since the monochromator is not required in the present invention, the waveguide unit is also unnecessary, and the laser beam used directly excites the fluorescent label.

The optical system of the present invention does not require a reflector. In previous designs, a reflector was used to guide the fluorescent beam to the detection unit. On the other hand, in the present invention, the fluorescent beam is sent directly to the PD sensor unit.

In the optical system of the present invention, the laser beam directly collides with the sample. In the previous design, the wavelength of the incident beam was selected using a dichroic mirror placed in front of the beam colliding with the sample. On the other hand, in the proposed method, the laser beam directly collides with the sample, so that the dichroic mirror can be omitted.

The present invention is an optical system for detecting fluorescent polarization, which is provided in a light source including a semiconductor laser diode that emits a beam, a collimating lens that is provided in the optical path of the beam and collimates the beam, and an optical path in the vicinity of the collimating lens. A polarizing plate that polarizes the beam, a sample table that includes a sample chamber and contains a sample mixed with a probe to induce fluorescence, and a degree of polarization measurement unit. , The polarization degree measuring unit is provided with a pinhole for passing the induced fluorescence from the sample, a pinhole plate for reducing scattered fluorescence, and the induced fluorescence passing through the pinhole is subjected to horizontal polarization-induced fluorescence and vertical polarization. The pinhole plate is mounted on the surface of the splitter, including a splitter that divides into induced fluorescence and two photoelectric detectors that accept the horizontal polarization induced fluorescence and the vertical polarization induced fluorescence to perform on-site detection. And the two photoelectric detectors relate to optical systems mounted on different surfaces of the splitter, respectively.

In a specific embodiment, the optical system further includes an optical filter provided between the collimating lens and the polarizing plate to filter a beam collimated by the collimating lens.

In a specific embodiment, the sample chamber is a circular tube tank or a square tank.

In a specific embodiment, the sample chamber is a glass tube unit, and the degree of polarization measuring unit is provided around the glass tube unit and outside the polarizing plate so that the induced fluorescence can be received at any angle.

In a specific embodiment, the light source is a plurality of light sources.

In a specific embodiment, the wavelengths of the plurality of light sources are the same or different.

In a specific embodiment, the optical system is portable.

In a specific implementation, the sample mixed with the probe has a complementary sequence of target DNA.

In a specific embodiment, the optical system is provided in the vicinity of one side of the sample table so as to enhance the ability to measure the fluorescence intensity, and another photoelectric detection perpendicular to the direction of the degree of polarization measurement unit. Including more vessels.

The present invention is a polarization degree measuring unit that detects fluorescence polarization, and is provided with a pinhole, a pinhole plate that allows fluorescence induced from a sample mixed with a probe to pass through, and induced fluorescence that passes through a pinhole. The pinhole plate comprises a splitter that divides the horizontal polarization-induced fluorescence into a vertical polarization-induced fluorescence, and two photoelectric detectors that accept the horizontal polarization-induced fluorescence and the vertical polarization-induced fluorescence, and the pinhole plate is one surface of the splitter. And the two photoelectric detectors relate to a degree of polarization measuring unit mounted on different surfaces of the splitter.

The present invention further comprises an optical system for detecting fluorescent polarization, which includes a light source including a semiconductor laser diode that emits a laser beam, and a first collimating lens provided in the optical path of the laser beam and collimating the laser beam. , A sample table provided in the optical path near the first collimating lens and provided with a first polarizing plate for polarizing the laser beam, a sample chamber for accommodating a sample mixed with a probe so as to induce fluorescence, and a degree of polarization detection. A unit and two second polarizing plates, each of which is provided on one side of the sample table so that the induced fluorescence is polarized into horizontal polarization-induced fluorescence and vertical polarization-induced fluorescence. Two second collimating lenses, each of which is provided in the vicinity corresponding to one of the two second polarizing plates and collimates the polarization-induced fluorescence, and each of the two collimating lenses. It receives the collimated horizontal polarization-induced fluorescence and the collimated vertical polarization-induced fluorescence obtained by collimating with two second collimating lenses, which are provided in the vicinity corresponding to one of the collimating lenses, and performs on-site detection. It relates to an optical system including two photoelectric detectors.

In a specific embodiment, the optical system further includes an optical filter provided between the first collimating lens and the first polarizing plate to filter a laser beam collimated by the first collimating lens.

In a specific embodiment, the two photoelectric detectors, the two second collimating lenses, and the two second polarizing plates are provided on both sides of the sample table, respectively, and are 180 ° or 90 ° with respect to the sample table. Positioned at °.

In a specific embodiment, the sample table is a glass tube or a square glass tube.

In the specific implementation plan, the number of the light sources is one or more, and the light sources are provided around the sample table.

In a specific embodiment, the wavelength of the semiconductor laser diode is the same or different.

In a specific embodiment, the optical system is portable.

The present invention further comprises an optical system for detecting fluorescent polarization of a sample, which includes a light source including a semiconductor laser diode that emits a laser beam, and a collimating lens provided in the optical path of the laser beam and collimating the laser beam. A first optical filter provided in the vicinity of the collimating lens to filter the collimated light, a polarizing plate provided in the optical path in the vicinity of the first optical filter to polarize the laser beam, and a polarizing plate for receiving the polarized beam are received. A sample chamber containing a sample chamber in which a sample mixed with a probe to induce fluorescence is housed in the sample chamber, and a degree of polarization detection unit, wherein the degree of polarization detection unit filters the induced fluorescence. Two optical filters, a polarization beam splitter that divides the induced fluorescence into horizontal polarization-induced fluorescence and vertical polarization-induced fluorescence, and a polarization beam splitter provided on both sides of the polarization beam splitter to accept the horizontal polarization-induced fluorescence and the vertical polarization-induced fluorescence. It relates to an optical system including two photoelectric detectors, which perform on-site detection.

In a specific embodiment, the sample table is a glass tube or a square glass tube.

In a specific embodiment, the optical system is portable.

FIG. 1A is an emission spectrum of a halogen lamp that diffuses over a wide range. FIG. 1B is an emission spectrum of a HID lamp that diffuses over a wide area. FIG. 1C is a diagram illustrating that selection of white light having a specific wavelength with a monochromator causes a decrease in intensity. FIG. 2 is a spectrum of a semiconductor laser diode with substantially monochromatic light. FIG. 3 is a typical polarization degree measuring system. FIG. 4 is a diagram showing an optical system for detecting fluorescent polarization in a specific embodiment of the present invention. FIG. 5 is a diagram showing an optical system for detecting fluorescent polarization whose detection angle can be adjusted by using the circular tube tank in one embodiment. FIG. 6 is a diagram showing an optical system for detecting fluorescent polarization in a specific embodiment of the present invention. FIG. 7 is a diagram showing an optical system for detecting fluorescent polarization provided with a circular tube tank according to an embodiment of the present invention. FIG. 8 is a diagram showing a plurality of polarized beam emitting units used for each fluorescent label in the embodiment of the present invention. FIG. 9 is a diagram showing a plurality of photoelectric detectors in one embodiment of the present invention. FIG. 10 is a diagram showing an example of specificity detection carried out on E. coli genomic DNA using the optical system for detecting fluorescent polarization of the present invention in one embodiment of the present invention. ..

In order to describe in more detail the technical means and effects that the present invention uses to achieve the intended purpose of the present invention, the following is a combination of drawings and preferred embodiments, the specific embodiments, structures, according to the present invention. The features and effects will be described in detail.

FIG. 4 shows an optical system for detecting fluorescent polarization in a specific embodiment of the present invention. As shown in FIG. 4, the polarized beam emitting unit 10 includes a semiconductor laser device (semi-conductor laser) 101, a collimator lens 102, an optical filter 103, and a polarizing plate (polarizer). Polarizer plate) 104. The semiconductor laser device 101 emits a polarized laser beam having a specified wavelength. The collimating lens 102 collimates the laser beam. After being collimated, the laser beam passes through the optical filter 103 and the polarizing plate 104. The sample is located in a square tank (square cell) 2a. Although the tank is square in FIG. 4, a circular tube tank may be used. The polarization degree measuring unit of FIG. 4 consists of a pair of detector units. Each detector unit includes a photodetector 31, a collimator lens 32, and a polarizing plate 33. Depending on the position of each detector unit, the polarizing plate 33 is positioned on the polarization plane on which the polarization axis is on the laser beam, while the other polarizing plate 33 is polarized so that the polarization axis is perpendicular to the laser beam. It can be positioned on a flat surface. Further, the output of the voltage from each photoelectric detector 31 is measured and recorded.

FIG. 5 shows a specific embodiment in which the detection angle can be adjusted by using the round-tube cell 2b. The polarized beam emitting unit 10 includes a semiconductor laser device 101, a collimating lens 102, an optical filter 103, and a polarizing plate 104. The sample is located in the circular tube tank 2b. The polarization degree measuring unit consists of a pair of detector units. Each detector unit includes a photoelectric detector 31, a collimating lens 32, and a polarizing plate 33, respectively. The photoelectric detector 31 is provided so as to form a right angle of 90 degrees.

FIG. 6 shows an optical system for detecting fluorescent polarization in a specific embodiment of the present invention. As shown in FIG. 6, the polarized beam emitting unit 10 is composed of a semiconductor laser device 101, a collimating lens 102, an optical filter 103, and a polarizing plate 104. The semiconductor laser device 101 emits a polarized laser beam having a specified wavelength. The collimating lens 102 collimates the laser beam. After that, the collimated laser beam passes through the optical filter 103 and the polarizing plate 104. The sample is located in the square tank 2a. Although the tank is square in this figure, a circular pipe tank may be used. The detector unit of FIG. 6 is composed of two photoelectric detectors 31, one polarization beam splitter 34 and one optical filter 35. The two photoelectric detectors 31 are provided so as to form 90 degrees.

FIG. 7 shows an optical system for detecting fluorescent polarization provided with a circular tube tank 2b in a specific embodiment of the present invention. As shown in FIG. 7, the polarized beam emitting unit 10 is composed of a semiconductor laser device 101, a collimating lens 102, an optical filter 103, and a polarizing plate 104. The semiconductor laser device 101 emits a polarized laser beam having a specified wavelength. The collimating lens 102 collimates the laser beam. After that, the collimated laser beam passes through the optical filter 103 and the polarizing plate 104. Since the sample is located in the circular tube tank 2b, the user can make the sample tube at any angle. The detector unit of FIG. 7 comprises two 90 degree positioned photoelectric detectors 31, a polarization beam splitter 34 and a pinhole plate 36. The pinhole plate 36 is mounted on the surface of the polarization beam splitter 34. Further, the photoelectric detector 31 is mounted on the surface of the polarization beam splitter 34. By mounting the pinhole plate 36 and the photoelectric detector 31 on the surface of the polarization beam splitter 34, optical calibration becomes easier and easier.

FIG. 8 shows a plurality of polarized beam emitting units 10 used for each fluorescent label in a specific embodiment of the present invention. The plurality of polarized beam emitting units have different wavelengths for selecting the incident beam.

FIG. 9 shows a plurality of photoelectric detectors 31 in a specific embodiment of the present invention. In the specific embodiment, the system can not only detect fluorescent polarization using a pair of separately added photoelectric detectors 31, but also simultaneously detect the intensity of the fluorescent beam to provide a photon absorption status. Is.

To prepare the sample, DNA is extracted from the target sample and then the DNA number is amplified by a standard PCR program. After amplifying the DNA, a fluorescent probe is added to the amplified DNA to prepare the sample for detection. Next, in order to operate the present system, the optical system of the present invention is connected to a computer device such as a notebook personal computer or a desktop personal computer via a connector, and then the measurement software of the optical system of the present invention is operated. Optimally, the software is in the form of a graphical user interface (GUI). Subsequently, the optical system of the present invention is activated, the unit containing the sample is placed on the sample table in the optical system of the present invention, and the fluorescence measurement is started. The fluorescence polarization value is displayed on the screen, and the Y-axis shows the mP value and the X-axis shows the time scale in seconds. Normally, the mP value stabilizes after 10 to 20 seconds. When the mP value is higher than the reference mP value provided for various DNAs as the reference value, it means that the target DNA is contained in the sample, but when the mP value is less than the reference value, the sample concerned. Means that the target DNA is not contained in.

FIG. 10 shows an example of specificity detection performed on Escherichia coli genomic DNA using the optical system of the present invention, which is a specific example described in FIG. 6 of Example 3. After non-target PCR amplification of the target DNA fragment, the degree of polarization was measured using the optical system of the present invention, and 102 to ¹⁰⁷ Escherichia coli genomic DNAs ^were detected. Based on the specificity of this E. coli detection test, when the genomic DNA of the introduced Salmonella or the DNA of the salmon sperm used (non-specific, negative control group DNA of random sequence) was detected, it is not necessary to consider the signal. It became clear.

The present specification describes the present invention in sufficient detail and provides examples to facilitate the manufacture and use of the present invention by those skilled in the art. Various replacements, modifications and improvements are self-evident, provided that they do not deviate from the spirit and scope of the present invention.

As can be understood by those skilled in the art, the present invention is very suitable for achieving the above-mentioned goals, and achieves the above-mentioned purposes and advantages, as well as specific purposes and advantages. It should be noted that the cells, animals, the manufacturing process and the method for preparing the cells are typical preferable concrete implementation methods, and are examples only, and do not limit the scope of the present invention. Those skilled in the art are familiar with the modification method and its application to other uses.

Claims (17)

An optical system that detects fluorescent polarization,
A light source, including a semiconductor laser diode that emits a beam,
A collimating lens provided in the optical path of the beam and collimating the beam,
A polarizing plate provided in an optical path near the collimating lens to polarize the beam,
A sample table that accepts a polarized beam and contains a sample chamber, in which a sample mixed with a probe to induce fluorescence is housed in the sample chamber, and
The polarization degree measuring unit includes the polarization degree measuring unit.
A pinhole plate provided with pinholes that allow fluorescence induced from the sample to pass through and reduce scattered fluorescence.
A splitter that divides the induced fluorescence that has passed through the pinhole into horizontal polarization-induced fluorescence and vertical polarization-induced fluorescence, and
Includes two photoelectric detectors that accept the horizontal polarization-induced fluorescence and the vertical polarization-induced fluorescence and perform on-site detection.
An optical system in which the pinhole plate is mounted on the surface of the splitter, and the two photoelectric detectors are mounted on different surfaces of the splitter, respectively. The optical system according to claim 1, further comprising an optical filter provided between the collimating lens and the polarizing plate and filtering a beam collimated by the collimating lens. The optical system according to claim 1, wherein the sample chamber is a circular tube tank or a square tank. The optical system according to claim 1, wherein the sample chamber is a glass tube unit, and the degree of polarization measuring unit is provided around the glass tube unit and outside the polarizing plate. The optical system according to claim 1, wherein the light source is a plurality of light sources. The optical system according to claim 5, wherein the wavelengths of the plurality of light sources are the same or different. The optical system according to claim 1, wherein the optical system is portable. The optical system according to claim 1, wherein the sample mixed with the probe has a complementary sequence of the target DNA. The optical system according to claim 1, further comprising another photoelectric detector provided in the vicinity of one side of the sample table and perpendicular to the direction of the degree of polarization measuring unit. A polarization degree measuring unit for an optical system that detects fluorescent polarization using a light source including a semiconductor laser diode.
A pinhole plate provided with pinholes to allow fluorescence induced from the sample mixed with the probe to pass through.
A splitter that divides the induced fluorescence that has passed through the pinhole into horizontal polarization-induced fluorescence and vertical polarization-induced fluorescence.
Includes two photoelectric detectors, the horizontal polarization-induced fluorescence and the vertical polarization-induced fluorescence.
A polarization degree measuring unit, wherein the pinhole plate is mounted on one surface of the splitter, and the two photoelectric detectors are mounted on different surfaces of the splitter, respectively. An optical system that detects fluorescent polarization,
A light source containing a semiconductor laser diode that emits a laser beam,
A first collimating lens provided in the optical path of the laser beam and collimating the laser beam,
A first polarizing plate provided in an optical path near the first collimating lens to polarize the laser beam,
A sample table that receives a laser beam polarized by the first polarizing plate, includes a sample chamber, and contains a sample mixed with a probe so as to induce fluorescence, and a sample chamber.
Including the degree of polarization detection unit,
The degree of polarization detection unit is
Two second polarizing plates, each provided on one side of the sample table, so as to polarize the induced fluorescence into horizontal polarization-induced fluorescence and vertical polarization-induced fluorescence.
Two second collimating lenses, each of which is provided at an adjacent position corresponding to one of the two second polarizing plates and collimates the horizontal polarization-induced fluorescence and the vertical polarization-induced fluorescence, and
The collimated horizontal polarization-induced fluorescence and the collimated vertical polarization-induced fluorescence obtained by collimating with the two second collimating lenses, each of which is provided at an adjacent position corresponding to one of the two second collimating lenses. An optical system comprising two photoelectric detectors, which accept fluorescence and perform on-site detection. The optical system according to claim 11, further comprising an optical filter provided between the first collimating lens and the first polarizing plate and filtering a laser beam collimated by the first collimating lens. The two photoelectric detectors, the two second collimating lenses, and the two second polarizing plates are provided on both sides of the sample table, respectively, and are provided at a position of 180 ° or 90 ° with respect to the sample table. The optical system according to claim 11. The optical system according to claim 12, wherein the sample table is a glass tube or a square glass tube. The optical system according to claim 14, wherein the number of the light sources is one or more, and the light sources are provided around the sample table. The optical system according to claim 15, wherein the wavelengths of the semiconductor laser diodes are the same or different wavelengths. The optical system according to claim 11, wherein the optical system is portable.

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