JP3234862U - Crystal structure of a combination of nutrients to promote the regulation of the intestinal flora and promote the body's antioxidant and anti-inflammatory effects - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a crystal structure of a nutritional combination that reduces the progress of skin aging, appropriately regulates the amount of bacteria in digestive system organs, and makes the body less likely to become inflamed for a long period of time. A nutritional combination comprising a spherical capsule 11, a film-coated body 12, and a nutritional combination 13 for promoting the regulation of the gut microbiota and promoting the body's antioxidant and anti-inflammatory effects. In the crystal structure 1, the film-coated body covers the combination of nutrients for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body, and the spherical capsule is the film-coated body. It covered the body with a combination of nutrients to promote the regulation of the gut microbiota and promote the antioxidative and anti-inflammatory effects of the body, and made the crystal structure spherical as a whole. [Selection diagram] Fig. 1

Description

The present invention relates to a drink containing a crystal structure, and more particularly to a crystal structure of a nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body.

As the times change, people meet high requirements in everything from their body appearance, skin quality, body shape and posture to their internal health, body fat percentage and metabolic rate. Pursuing the perfect body to get.
And because health is essential to maintain a beautiful appearance and body, people pay more attention to health care and their own physical health care, and are in the best condition from the inside of the body to the appearance and appearance. I want.

By the way, there are many kinds of modern dishes, and people eat all kinds of dishes including meat, starch, carbohydrates and other ingredients on a daily basis.

However, the intake of foodstuffs that aid digestion such as vegetables and fruits is much smaller than the intake of foodstuffs that are difficult to digest such as meat, and as a result, the burden of digestion of the intestines is often high.
In addition, modern people are overwhelmed by many jobs and suffer from excessive pressure for a long period of time, and there is a lot of stress in continuing their lives. In addition to these factors, eating out with fast food, which is often difficult to digest, also adversely affects the functions of the endocrine system and digestive system in the body, so toxins in the body accumulate in the intestine and the intestinal barrier. It may damage the mucous membranes that function as a function, interfere with the contractile movement of the intestines, and adversely affect the absorption of nutrients.

In addition, if the mucous membrane, which acts as an intestinal barrier, is damaged, the toxins pass through the intestinal barrier and mix with the blood. When a toxin is mixed in blood, the human body functions to eliminate this toxin, but at that time, inflammation occurs. Furthermore, not only inflammation occurs, but when harmful substances accumulate, fat tends to accumulate in the lower abdomen, which accelerates the progress of skin aging, causes dullness in the skin, and tends to cause acne and pimples. It is known that, in severe cases, it causes bad breath, causes malaise, and is prone to constipation. In other words, in order not to cause these problems, it is important to keep the intestinal environment as normal as possible in order to maintain good health.

In view of the above problems, the present invention is a combination of nutrients that reduces the progress of skin aging, appropriately regulates the amount of bacteria in the digestive system organs, and makes the body less likely to become inflamed for a long period of time. It is an object of the present invention to provide a crystal structure.

The present invention has the following configurations in view of these problems. That is,
Spherical capsules, film-coated bodies, and a combination of nutrients to promote the regulation of the gut microbiota and to promote the body's antioxidant and anti-inflammatory effects, as well as facilitating the regulation of the gut microbiota. A crystalline structure of a combination of nutrients to promote the body's antioxidant and anti-inflammatory effects.
The film-coated body covers the nutritional combination to promote the regulation of the intestinal flora and promote the body's antioxidant and anti-inflammatory effects.
The spherical capsule covers the film-coated body and a nutritional combination for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant and anti-inflammatory effects of the body, and makes the crystal structure spherical as a whole. ..

In addition, the diameter size of the crystal structure of the combination of nutrients for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant action and anti-inflammatory action of the body is 1 μm to 10 μm.
In addition, the combination of nutrients for promoting the regulation of the intestinal flora and the antioxidant and anti-inflammatory effects of the body is a combination of yam bean enzyme and acai enzyme fermented with light of a specific wavelength. ..

In addition, the yam bean enzyme and the acai enzyme fermented with light of a specific wavelength are in the form of powder.
In addition, the nutritional combination for promoting the regulation of the gut microbiota and promoting the body's antioxidant and anti-inflammatory effects further comprises probiotics, which are in the form of powder.

In addition, the spherical capsule is pectin or other edible colloid.

In addition, the crystal structure of the nutritional combination for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant action and anti-inflammatory action of the body is translucent.

In addition, the crystalline structure of the nutritional combination to promote the regulation of the gut microbiota and promote the body's antioxidant and anti-inflammatory effects is mixed with health products composed of drinks, colloids, or troches. Can be done.

The present invention has the following effects. That is, modern humans can promote the regulation of the intestinal flora and promote the body's antioxidant and anti-inflammatory abilities by utilizing the crystal structure of the nutritional combination according to the present invention. In addition, the crystal structure of the nutritional combination according to the present invention makes it difficult for the anti-inflammatory nutritional composition to be destroyed by gastric acid, so that the nutritional combination according to the present invention reaches the intestine as it is and promotes intestinal health. In addition, the crystal structure according to the present invention exerts an anti-inflammatory effect and prevents autoimmune diseases such as leaky gut.

In addition, the present invention provides a crystal structure of a combination of nutrients for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body, and the harmful effects accumulated in the modern human body. Improves the adverse effects of substances on the skin and intestines.
Here, in general, the combination of nutrients for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant action and the anti-inflammatory action of the body is easily destroyed when it comes into contact with digestive juice such as gastric acid.

Therefore, the crystalline structure of the combination of nutrients for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body according to the present invention can be used by modern humans even if it comes into contact with gastric acid. The combination of nutrients is not easily damaged and can enter the intestine relatively easily and promotes intestinal health, resulting in less inflammation in the body and toxins destroying the intestinal mucosa. It is possible to suppress the phenomenon of stagnation and enhance one's own immunity.

Since the present invention has the above-mentioned characteristics, it is possible to suppress the adverse effects of harmful substances accumulated in the human body on the skin and intestines.

In addition, as mentioned above, the combination of nutrients to promote the regulation of the general gut microbiota as well as the body's antioxidant and anti-inflammatory effects is easily destroyed as it passes through the stomach. However, since the crystal structure of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body according to the present invention is covered with a spherical capsule and a film coating body. Since it is not easily destroyed by contact with gastric fluid, it easily invades the intestines (without destroying the nutritional combination), promotes intestinal health management, and suppresses the entry of harmful substances into the intestines, which in turn prevents harmful substances from entering the intestines. It has the effect of enhancing autoimmune function.

FIG. 5 is a partial cross-sectional view showing an embodiment of a crystal structure of a nutritional combination for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body. This is an experimental result that can delay the inflammation of enterocytes according to the embodiment of the present invention. This is an experimental result for enhancing the intestinal barrier function according to the embodiment of the present invention. This is an experimental result of forming a colony of the bacterium of the present invention. FIG. 5A shows the experimental results of physical bacteria, and FIG. 5B shows the experimental results of Lactobacillus lactobacillus. FIG. 6A shows the experimental results of Ruminococcus, and FIG. 6B shows the experimental results of Prevotella. This is the experimental result of the number of defecations. FIG. 8A is the experimental result of hiccups, FIG. 8B is the experimental result of bloating, and FIG. 8C is the experimental result of nausea. It is the result of the total score of overall gastrointestinal discomfort according to the present invention. This is the experimental result of total antioxidant capacity (TAC) according to the present invention. This is an experimental result showing how much inflammation can be reduced by the present invention. This is an experimental result showing how much wrinkles are reduced by the present invention. This is an experimental result showing how much the fineness of the skin is improved by the present invention.

Hereinafter, embodiments of the present invention will be further described with reference to the drawings. It should be noted that the following embodiments are used only for explaining the contents of the present invention, and the technical scope of the present invention is not limited to the contents of the following embodiments, unless they deviate from the spirit of the present invention. Even if a person skilled in the art makes a simple addition or change, the product using the modified device shall belong to the technical scope of the present invention, and the technical scope shall be determined based on the scope of the utility model registration claim. It shall be.

With reference to FIG. 1, the structure of the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant action and the anti-inflammatory action of the body will be described.

The crystal structure 1, which is a combination of nutrients to promote the regulation of the intestinal flora and the anti-inflammatory and anti-inflammatory effects of the body, regulates the spherical capsule 11, the film-coated body 12, and the intestinal flora. Includes a nutritional combination 13 to promote and promote the body's antioxidant and anti-inflammatory effects.

The film-coated body 12 is coated so as to surround the nutritional combination 13 for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body. The spherical capsule 11 covers the nutritional combination 13 and the film-coated body 12 to promote the regulation of the intestinal flora and the antioxidant and anti-inflammatory effects of the body from the surroundings.

The crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body promotes the regulation of the intestinal flora and the antioxidant of the body. Prevents the combination of nutrients 13 for promoting action and anti-inflammatory action from being destroyed by digestive juices such as gastric acid, and promotes the regulation of the intestinal flora by the human body, as well as the antioxidant and anti-inflammatory effects of the body. Helps absorb the nutritional combination 13 to promote action.

The crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body is dissolved in water to form a uniformly dispersed solution. The size of the crystal structure 1 of the combination of nutrients to promote the regulation of the intestinal flora and the antioxidant and anti-inflammatory effects of the body is such that the sensation of particles is not easily felt when drinking a beverage. Suitable for use or sale of beverages in containers.

In a preferred embodiment of the present invention, the diameter of the sphere of the nutritional combination 13 for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body is 1 μm to 1 mm.

In the crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body, the regulation of the intestinal flora and the antioxidant of the body are promoted. The core diameter of the sphere of the nutritional combination 13 for promoting action and anti-inflammatory action is 0.1 μm to 0.2 mm.

The core of the sphere of the nutritional combination 13 for promoting the regulation of the intestinal flora and promoting the body's antioxidant and anti-inflammatory effects comprises at least one of the following, i.e., specific. Includes yam bean enzyme, acai enzyme, probiotics (Chinese notation eubacteria), and prebiotics (Chinese notation merits) that are emitted by light of wavelength.

Here, probiotics improve the intestinal environment by the action of the bacteria themselves, whereas prebiotics improve the intestinal environment by ingesting food components that feed useful intestinal bacteria. Although the two are strictly different, they may be referred to as probiotics, especially in claims.

In a preferred embodiment of the present invention, the film-coated body 12 is a carbohydrate composition, which is a nutrient for promoting the regulation of the intestinal flora and promoting the body's antioxidant and anti-inflammatory effects. Combination 13 is first used to coat (essentially, in direct contact with reference numeral 13 inside the spherical capsule 11).

In another preferred embodiment of the present invention, the film-coated body 12 is an oligosaccharide composition, and the oligosaccharide composition further contains fructooligosaccharide (FOS), xylooligosaccharide, and inulin (chicory fiber). The chicory fibers of the film-coated body 12 are extracted from the roots of chicory.

In embodiments of the present invention, chicory fibers can be used as prebiotics (Chinese notation benefits) to promote the growth of probiotics (Chinese notation benefits) in the body.

The dietary fiber of the carbohydrate composition of the present invention promotes the regulation of the intestinal flora and the regulation of nutritional combinations 13 to promote the body's antioxidant and anti-inflammatory effects. The spherical core is destroyed by gastric acid. Prevents it from being squeezed and reaches the intestines smoothly.

In addition, dietary fiber with a carbohydrate composition stimulates the intestines to produce protective mucus, which improves the movement of the intestines and slows down the residence time of food.

In a preferred embodiment of the present invention, the yam beans fermented by light having a specific wavelength are yam beans fermented by light having a wavelength of 600 mm to 680 mm, whereby the rate of metabolism of microorganisms is increased and the growth of microorganisms is increased. The speed will also increase. Yam beans fermented with light of a particular wavelength can reduce the content of free radicals in skin cells and promote intestinal probiotic colonization.

In a preferred embodiment of the present invention, the acai enzyme includes blueberries, cranberries, white radishes, carrots, bayberries, mulberry, grapes, apples, sugar cane, passion fruit, pineapple, lemon, broccoli, celery, asparagus and the like. It contains 15 kinds of abundant acai.

In addition, the assy enzyme of the present invention contains vitamins, polysaccharides, amino acids, various nutritional metabolites, digestive enzymes and the like, and is rich in phytochemicals. Superoxide dismutase (SOD) is also included in the crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal bacterial flora containing the acai enzyme of the present invention and promoting the antioxidant and anti-inflammatory effects of the body. include.

Also, in a preferred embodiment of the present invention, the spherical capsule 11 is an edible colloid. Colloids are natural macromolecular compounds found in various plants, are supporters of plant cells, and are translucent.

In another embodiment of the present invention, apple pectin (colloid) is selected from apples as the spherical capsule 11. The diameter of the spherical capsule 11 is 1 μm to 1 mm. Spherical capsule 11 contains indigestible dextrin, concentrated apple juice, citric acid, and concentrated berry juice.

Example 1: Detection of Nitric Oxide with Anti-Inflammatory Ability In order to investigate whether the particle structure of the present invention has an effect of reducing inflammation, in this example, intestinal cells C2BBe1 and macrophages RAW264.7 were used for intestinal inflammation. Co-cultured as a platform for. Lipopolysaccharide (LPS) induces RAW264.7 to release nitric oxide (NO), an pro-inflammatory mediator. The situation of intestinal inflammation is simulated, the production of the inflammatory responsive factor nitric oxide (NO) is detected, and the extent to which the sample can reduce the inflammatory response by passing through the intestinal mucosa is evaluated.

Materials and measuring equipment 1. Cell line: Human colon cell C2BBe1, obtained from the American Type Culture Collection (registered trademark ATCC, Cat.CRL-2102). Obtained from mouse macrophages (RAW264.7, registered trademark ATCC, Cat.TIB-71).

2. Medium: DMEM modified medium (purchased from Dulbecco's modified Eagle's medium, DMEM, Gibco, Cat.11965-092) is added to the additional components, and the additional components are from 10vol% FBS (fetal bovine Serum, Gibco, Cat.10437-028). Includes (purchased) and 1% penicillin-streptomycin (purchased from Gibco, Cat. 15140122).

3. 3. Phosphate buffered saline (PBS solution): Purchased from Gibco, product number Cat.14200-075.

4. Lipopolysaccharide (LPS): Purchased from Sigma, product number is SI-L2880-25MG.

5. Grease reagent kit: Purchased from Life technologies. The product number is 1445263.

6. Crystal structure of a combination of nutrients for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body: produced by the equipment method of Example 1 of the present application.

Experimental step:
1. 1. The experiment was divided into three groups: a control group, a control group (without adding the crystal structure according to the present invention and adding LPS medium), and an experimental group (without adding the crystal structure according to the present invention and adding LPS medium). Separately, each group was repeatedly tested three times.

2. C2BBe1 cells were seeded in a 24-well plate permeable cell culture chamber (registered trademark transwell) at ^{a density of 5 × 10 4} cells / well and the cells were cultured in an upper culture chamber (insert). The volume of the medium solution was 400 μl / well, and the cells were cultured in a constant temperature incubator of _{37 ° C. and 5% CO 2 for 24 hours.}

3. 3. RAW264.7 cells were planted in a 24-well plate permeable cell culture chamber (registered trademark transwell) for cell culture at ^{a density of 2 × 10 4} cells / well. The culture solution volume was 1000 μl / well, and the cells were cultured in a constant temperature incubator at 37 ° C. and 5% CO ₂ for 24 hours.

4. After removing the medium in the upper culture chamber (insert), it is hung in 24 wells planted with RAW264.7. LPS (concentration 1 μg / mL) is added to the experimental and blank control groups. At the same time, 5 μL of this crystal structure obtained in Example 1 of the present application is added to the experimental medium of the experimental group and allowed to act for 16 to 18 hours. (Use a medium sequence that does not contain FBS)
4. Take a 96-well plate and add 130 μl of secondary water to each well. After the reaction, 150 μl of culture is removed from each well of step 3 and added to a 96-well plate already containing 130 μl of secondary water.

5. Place a Griess reagent (reagent A: B = 1: 1), add 20 μl of medium to each well of a 96-well plate, and react in the dark for 30 minutes.

6. An ELISA reader (purchased from BioTek) is used to measure the absorbance at the OD548 nm wavelength (the higher the OD reading, the higher the concentration of NO).

7. Perform statistical analysis using student student t-test (* Table p <0.05, ** Table p <0.01, *** Table p <0.001).

-Experimental results Relative to nitric oxide produced after the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body by this device is processed. See FIG. 2, which is the change in quantity. Here, nitric oxide is an important inflammatory reaction factor. Therefore, in this embodiment, the amount of nitric oxide produced is used as an indicator of inflammatory response capacity.

As shown in FIG. 2, assuming that the amount of nitric oxide (NO) produced in the control group as compared with the control group is 100%, nitric oxide produced in the control group (that is, LPS is not added). The amount of is 45.8%.
In addition, the amount of nitric oxide produced in the experimental group was 76.9% as compared with the control group. From the results of this experiment, it can be seen that the experimental group reduced the production of nitric oxide by about 23.1% as compared with the control group.

It is inflamed after RAW264.7 macrophages have been treated by the crystal structure 1 of a nutritional combination to promote the regulation of the gut microbiota according to the present invention and to promote the body's antioxidant and anti-inflammatory effects. It means that the production of reaction factor (NO) can be reduced. That is, it is shown that the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant action and the anti-inflammatory action of the body according to the present invention has an effect of resisting inflammation.

Example 2: Intestinal permeability test (TEER)
The crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body has an effect of reducing intestinal permeability induced by inflammation. To determine if, in this example, enterocytes C2BBe1 are used as a platform for intestinal inflammation, which is simulated by lipopolysaccharide (LPS) to induce C2BBe1 and increase intestinal permeability. , Detect transepithelial electrical resistance (TEER) of enterocyte C2BBe1 to assess whether the sample can enhance the effect of the intestinal barrier.

Human colon cell C2BBe1 is ^{placed in a 24-well plate at a density of 1 × 10 4} cells / well and cultured for 7-14 days to measure the cell transmembrane resistance (TEER).
In this experiment, the experimental group (added the crystal structure of the present invention and added LPS (concentration: 500 ng / mL) medium), the control group (without adding the crystal structure of the present invention, LPS (concentration: 500 ng / mL)). (Medium) and control group (without adding the crystal structure according to the present invention and without adding LPS medium).

Each group performed three iterative tests, measured using a transmembrane resistance meter (Millicell-ERS Voltohmmeter), inserted the sensor of the transmembrane resistance meter into the upper culture chamber, and the other end of the detection. Stretch into the lower culture chamber for. Then, after the value stabilizes, the resistance measurement value is recorded. Then, the TEER value is obtained by subtracting the measured value of the upper culture chamber containing no cells. As a result, the more complete the cell-binding protein, the higher the resistance and TEER values.

This will be described with reference to FIG. FIG. 3 is a TEER value change chart after processing the crystal structure of the present invention. When the TEER value of the control group is 100%, the control group after adding LPS is compared with the control group. The TEER value decreased to 86.6%.

From this, it can be understood that the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant action and the anti-inflammatory action of the body according to the present invention has the following effects.

That is, regarding the disruption of the enterocyte barrier, the TEER value in the experimental group increased by 108.7% after the addition of LPS and 22.1% in the control group as compared with the control group.

This means that it can increase the integrity of the enterocyte barrier, effectively maintain the intestinal barrier, prevent harmful substances from passing through the intestinal barrier and mixing with the blood, preventing self-immune diseases. It means that it can be done and the symptoms can be alleviated.

Example 3: Intestinal colonization experiment In Example 3, human colon cells C2BBe (registered trademark ATCC CRL-2102 ^TM ) and Lactobacillus plantarum TCI227 (April 15, 2019, biological resources of the Institute for Food Industry Development) The colony-forming state was co-cultured with BCRC910884, which was provided by the Conservation Research Center and is described in Taiwanese proprietary application No. 108123471. The colony formation state of Lactobacillus plantarum TCI227 was confirmed by observing and analyzing the colony formation rate by the plate counting method.

First, to obtain the culture 6-well plates were placed in colonies wells human colon cells in a ratio of 7.5 × 10 ⁵ cells per well, the culture of 2 ml (mL) was added to each well, 5% Put in carbon dioxide. Then, it was concentrated under the temperature condition of 37 ° C. and incubated in the incubator for 24 hours.

Next, the secondary activated bacterial solution prepared in Experiment 1 and the MRS medium were used to generate a bacterial solution containing Lactobacillus plantarum TCI227 (hereinafter referred to as 1OD recent solution) with OD600 = 1OD. Here, OD600 is an absorbance value (OD value) measured at a wavelength of 600 nm by an enzyme-linked immunosorbent assay reader (ELISA Reader).

Centrifuge the 1OD bacterial solution to collect the bacteria of Lactobacillus plantarum TCI227. Then use the C2BBe1 medium without antibiotics, it adjusts the collected bacteria in experimental bacterial solution of 10 8 ^CFU / ml.

After removing the culture medium in the culture plate, 1 ml (mL) of experimental bacterial solution was added to each well of the experimental group, and 1 mL of C2BBe1 medium (excluding antibiotics and experimental bacterial solution) was added to the control group. Added to each well.

All groups were then simultaneously incubated for 1 hour in a hypoxic environment (oxygen content less than 1%).

Next, after Gram stain (staining reagent was purchased from BaCO Biotech) on human colon cells and Lactobacillus plantarum TCI227 in the experimental and control groups, 1 ml (mL) of Triton X-100 (nonionic surfactant) The agent) was added to each well of the experimental group and the control group, and the reaction was carried out at room temperature. Here, 10 minutes are required to remove human colon cells and Lactobacillus plantarum TCI227 from the culture plate. It was then spread on an agar gel plate using the diluted smearing method and the colony formation rate was analyzed by the plate counting method.

This will be described with reference to FIG. When the colony formation rate of Lactobacillus plantarum TCI227 was 100% obtained by the pan coating machine as in the control group of FIG. 4, the colonies of Lactobacillus plantarum TCI227 in the experimental group were compared with those in the control group. The formation rate was 149.7%, an increase of 49.7%. From this, it can be seen that stable colonies can be formed on Lactobacillus plantarum TCI227 human colon cells.

In order to verify the effect of the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body on the human body according to the present invention, also for humans. The following experiment was performed.

Example 4: Human subjects-regulating the gut microbiota In this example, 9 subjects (generally men and women aged 23-45 years) were asked for a total of 4 weeks per day. Dew manufactured so that one drink (50 mL) containing crystal structure 1 of a nutritional combination for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant and anti-inflammatory effects of the body according to the present invention can be ingested. I had them drink berry extract.

In the above subjects, the crystal structure of the 0th week (in short, a combination of nutrients for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body). Crystal structure of a combination of nutrients to promote the regulation of the gut microbiota according to the present invention and to promote the body's antioxidant and anti-inflammatory effects (before drinking the above drink containing 1) and the 4th week (1) We asked them to experiment with what the gut microbiota would look like after drinking the above drinks containing.

The detection method is to collect a sample of the target stool and extract DNA from the stool to detect the proportion of common gut microbiota from the primer sequence of known bacteria to detect the 16S ribosomal gene. It is used to perform a quantitative real-time polymerase chain reaction (qRT-PCR).

In this detection method, DNA extraction is performed from feces and 16S Amplicon Sequencing of microorganisms on a sample is performed using the Next Generation Sequencing (NGS). Here, especially the V3-V4 region in 16S DNA is amplified and subsequent sequencing analysis is performed.

This embodiment is carried out by Tursi Biotechnology Co. Ltd. in a next-generation sequencing method, and the sequencing of 16S Amplicon Sequencing of microorganisms is performed at both ends. Performed using Paired-End. Use lead splicing and filtering and OTU (Operational Taxonomic Units) clustering to perform race (species) annotations "Chinese notation species annotations" and abundance analysis. In addition, the composition of the seeds of the sample will be disclosed, and the types and proportions of microorganisms in the sample will be analyzed.

This will be described with reference to FIGS. 5A to 5B. These FIGS. 5A and 5B show the crystal structure of a combination of nutrients for promoting the regulation of the intestinal flora according to the present invention for increasing intestinal probiotics and promoting the antioxidant and anti-inflammatory effects of the body 1. It shows the effect of. Faecalibacterium is an intestinal probiotic that can stimulate the production of short-chain fatty acids (butyric acid). By producing butyric acid, intestinal health can be promoted.

Lactobacillus is a type of probiotic that can ferment carbohydrates with lactic acid to aid digestion and help human gut health.

Assuming that the proportion (relative abundance) of bacterial species before taking (in short, the 0th week) is 100%, as shown in FIG. 5A, the regulation of the intestinal flora according to the present invention is promoted and the body is antioxidant. Drinking a drink containing crystal structure 1 of a nutritional combination to promote action and anti-inflammatory action for 4 weeks can increase the proportion (relative abundance) of Faecalibacterium strains to 123%. did it. Further, as shown in FIG. 5B, the proportion (relative abundance) of the Lactobacillus strain increased to 385%.

With reference to FIGS. 6A and 6B, the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body has the effect of reducing bad bacteria. Is shown. Ruminococcus is a pathogen that breaks down intestinal mucus, causing endogenous intestinal infections and increasing the likelihood of intestinal pain.

Prevotella helps cells absorb carbohydrates and gain weight. When the proportion (relative abundance) of bacterial species before taking (week 0) is 100%, it promotes the regulation of the intestinal flora according to the present invention and promotes the antioxidant and anti-inflammatory effects of the body. After drinking a drink containing Crystal Structure 1 of the nutritional combination for 4 weeks, the proportion (relative abundance) of Ruminococcus could be reduced to 56%. Therefore, in FIG. 6A, the notation of 44% down is made. In addition, the proportion (relative abundance) of Prevotella decreased to 78%. Therefore, in FIG. 6B, the notation of 22% down is made.

The above combination is the subject's gastrointestinal tract after drinking a drink containing crystal structure 1 of a nutritional combination to promote the regulation of the gut microbiota according to the present invention and to promote the antioxidant and anti-inflammatory effects of the body. Probiotic bacteria have increased significantly, indicating a significant decrease in bad bacteria.

In addition, the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant and anti-inflammatory effects of the body according to the present invention regulates the microbial flora and probiotics in the intestine. It has the effect of increasing the proportion of bacteria and promoting gastrointestinal health.

Example 5: Experiment conducted on a human subject-Inspection of severity of gastrointestinal condition In Example 4, the gastrointestinal condition of the subject was evaluated. Prior to the start of the experiment, each subject completed a questionnaire on gastrointestinal status at week 0 to collect the number of bowel movements, hiccups, abdominal distension, and nausea severity of each subject at week 0.

The subject was then given a drink containing Crystal Structure 1 of a nutritional combination to promote the regulation of the gut microbiota according to the present invention and to promote the body's antioxidant and anti-inflammatory effects daily for 4 weeks. I drank one by one. After that, when I asked about the gastrointestinal condition after the 4th week had passed, the contents were the same as the 0th week.

Hiccups, bloating, and nausea were scored 0-6, from the least severe to the most severe, depending on their severity. Finally, a total score for gastrointestinal discomfort was calculated based on all scores for hiccups, bloating, and nausea.

The questionnaire on gastrointestinal condition at week 0 and the questionnaire on gastrointestinal condition at week 4 were analyzed. The results are shown in FIGS. 7 to 9. At week 4, the scores for bowel movements, hiccups, bloating, and nausea were significantly reduced.

FIG. 7 shows that the number of defecations increased from 4.4 times a week in the 0th week to 9.1 times a week after the 4th week, so that it increased by 4.7 times.

8A-8C show a 100% severity score for hiccups, abdominal distension, and nausea in week 0 subjects. Based on this, the severity of hiccups decreased to 42.6% after the 4th week, the severity of abdominal distension decreased to 58% after the 4th week, and the severity of nausea was 4. It decreased to 44.1% in the week.

FIG. 9 shows that the total score of overall gastrointestinal discomfort dropped to 53.5% at week 4. Therefore, the notation that the decrease is 46.5% is shown in FIG.

The above results show that the crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body increases the number of defecations, as well as hiccups and abdomen. It has been shown to be effective in reducing bloating, nausea, and other gastrointestinal discomfort.

Example: Experiment conducted on a human subject-Determining total antioxidant capacity In this experiment, nutrition for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body. Blood is collected from the subjects of Example 4 after the 0th and 4th weeks in order to detect changes in the total antioxidant capacity (value) in the blood before and after ingesting the beverage containing the combination crystal structure 1. The data was acquired by this.

The Total Antioxidant Capacity (TAC) test measures the overall antioxidant capacity of non-enzymatic antioxidants in plasma and is more systemic than measuring only specific antioxidants. It reflects the oxidizing ability in an easy-to-understand manner.

And the higher the measured TAC value, the higher the antioxidant capacity in the body. The TAC value in the blood of the subjects of this example was measured by the Commissioner's Laboratory (Taiwan).
In the 0th week (in short, before drinking) of drinking a drink containing crystal structure 1 of a nutritional combination for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body. The result of analyzing the change in the total antioxidant capacity of the subject after the lapse of the 4th week is shown in FIG. Here, assuming that the total antioxidant capacity (TAC) value of the subject at week 0 was 100% for comparison, the total antioxidant capacity of the subject who continued to drink for 4 weeks was 103.5%. It can be seen that there was an increase of 5%.

Example 7: Experiment conducted on a human subject-Measurement of the degree of inflammation In this experiment, nutrition for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body. Data were obtained by collecting blood from the subjects of Example 4 after the 0th and 4th weeks in order to detect changes in the degree of inflammation before and after drinking the beverage containing the combination crystal structure 1.

C-reactive protein (CRP) is a special protein produced in the liver, which is an acute and non-specific reactive protein (acute phase oxidizing protein), which is a tissue destruction in the process of acute inflammation. It becomes an index.

Using enzyme-linked immunosorbent assay (ELISA) for the measurement, it was found that the higher the level of C-reactive protein in the measured blood, the more severe the anti-inflammatory response in the body. The content of C-reactive protein in the blood of the subject in this example was measured by the Commissioner's Laboratory (Taiwan).

Weeks 0 and 4 of subjects who drank a drink containing crystal structure 1 of a nutritional combination to promote the regulation of the intestinal flora according to the present invention and to promote the antioxidant and anti-inflammatory effects of the body. Analyze later changes in the degree of inflammation.

The results are shown in FIG. Assuming that the C-reactive protein content at week 0 was 100%, the subject's C-reactive protein content decreased to 62.3% after the 4th week, which was 37.7% of that before drinking. Diminished.

Example 8: Experiment conducted on a human subject-Measurement of skin wrinkles Using a VISIA high-end digital skin quality tester sold by Canfield in the United States, the regulation of the intestinal flora according to the present invention is promoted. The same subject as in Example 4 after the 0th week before drinking and the 4th week after drinking the beverage containing the crystal structure 1 of the combination of nutrients for promoting the antioxidant and anti-inflammatory effects of the body. The skin of the face was inspected.

The test method was to take high-resolution skin images with visible light and use built-in software to analyze skin roughness according to sunken (recessed) and raised (protruded) skin. According to this analysis method, the higher the measured value, the more rough the skin and the more wrinkles.

This will be described with reference to FIG. This graph shows the 0th week before drinking and the drink containing the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant and anti-inflammatory effects of the body. It shows the change in the skin wrinkles of the subject at the lapse of the 4th week after that, and the skin wrinkles of the subject at the 0th week before drinking were set to 100%.

Then, when a drink containing a crystal structure 1 of a nutritional combination for promoting the regulation of the intestinal flora according to the present invention and promoting the antioxidant action and the anti-inflammatory action of the body is taken, the subject is taken 2 weeks after use. The wrinkles or roughness of the skin of the subject decreased to 94.2%, and the wrinkles of the subject's skin decreased to 91.5% 4 weeks after use. That is, it means that if you continue to drink for 4 weeks, it will decrease by 8.5%. From this, the crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal bacterial flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body reduces the number of wrinkles on the skin and wrinkles. It has the effect of significantly reducing the area where wrinkles are formed, making wrinkles less noticeable, making the skin smoother, and suppressing the aging phenomenon of the skin.

Example 9: Experiment conducted on a human subject-Measurement of skin wrinkles Preparation of the intestinal flora of the present invention using a VISIA high-end digital skin quality tester sold by Canfield in the United States. I finished drinking the beverage containing the crystal structure 1 of the combination of nutrients to promote the antioxidative effect and anti-inflammatory effect of the body in the 0th week before drinking and the 2nd week after starting to drink. At the end of the 4th week, facial skin was tested on the same subjects as in Example 4. The test method was performed by taking a high-resolution skin image with visible light and using the built-in software to analyze the roughness of the skin according to the concave and convex surfaces of the skin. Here, the higher the measured value, the worse the rough skin and wrinkles.

An embodiment of the present invention will be described with reference to FIG. The graph of FIG. 13 shows the subjects before and after ingesting the beverage containing the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body. It shows the roughness of the skin, and when the roughness of the subject in the 0th week before starting to drink is 100%, the roughness of the subject's skin 2 weeks after the start of use is 93.7. The skin roughness of the subjects was 92% after 4 weeks of continuous use.

That is, if the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal bacterial flora of the present invention and promoting the antioxidant action and the anti-inflammatory action of the body is continuously taken for 4 weeks, the roughness of the skin is 8%. It can be seen that it decreases. Drinking a drink containing crystal structure 1 of a nutritional combination to promote the regulation of the intestinal flora of the present invention and promote the antioxidant and anti-inflammatory effects of the body enhances the fineness and smoothness of the skin. It can be seen that the skin tends to be supple.

As described above, the crystal structure 1 of the combination of nutrients for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant action and anti-inflammatory action of the body suppresses the aging phenomenon of the skin and the intestine. It can strengthen the barrier function of the skin, regulate the intestinal flora, reduce gastrointestinal discomfort, increase the body's antioxidant capacity, and enhance its anti-inflammatory effect.

In addition, the spherical capsule 11 of the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant action and the anti-inflammatory action of the body promotes the regulation of the intestinal flora. Together with it, it is translucent, wrapping it over a nutritional combination 13 to promote the body's antioxidant and anti-inflammatory effects.

In addition, the combination of nutrients 13 for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body is powdery and fermented with light of a specific wavelength. It is a combination of enzymes. Further, the film coating 12 is an oligosaccharide composition, and the oligosaccharide composition contains fructooligosaccharides, xylooligosaccharides and chicory fibers.

The spherical capsule 11 is an edible colloid. The size of the diameter of the crystal structure 1 of the nutritional combination for promoting the regulation of the intestinal flora of the present invention and promoting the antioxidant and anti-inflammatory effects of the body is 1 μm to 10 mm, and it is mixed with a drink and drunk. It is a product that can promote health such as colloids and troches (tablets).

1 Crystal structure of a combination of nutrients to promote the regulation of the intestinal flora and the antioxidant and anti-inflammatory effects of the body 11 Spherical capsule 12 Film-coated body 13 Promote the regulation of the intestinal flora and the body A combination of nutrients to promote antioxidant and anti-inflammatory effects

Claims (8)

Spherical capsules, film-coated bodies, and a combination of nutrients to promote the regulation of the gut microbiota and to promote the body's antioxidant and anti-inflammatory effects, as well as facilitating the regulation of the gut microbiota. A crystalline structure of a combination of nutrients to promote the body's antioxidant and anti-inflammatory effects.
The film-coated body covers the nutritional combination to promote the regulation of the intestinal flora and promote the body's antioxidant and anti-inflammatory effects.
The spherical capsule covers the film-coated body and a nutritional combination for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body, and makes the crystal structure spherical as a whole. A crystal structure of a combination of nutrients to promote the regulation of the gut microbiota and promote the body's antioxidant and anti-inflammatory effects. The first aspect of the present invention is characterized in that the diameter size of the crystal structure of the combination of nutrients for promoting the regulation of the intestinal bacterial flora and promoting the antioxidant and anti-inflammatory effects of the body is 1 μm to 10 μm. The crystal structure of a combination of nutrients to promote the regulation of the intestinal flora and promote the body's antioxidant and anti-inflammatory effects. The combination of nutrients for promoting the regulation of the intestinal flora and the antioxidant and anti-inflammatory effects of the body is a combination of yam bean enzyme and acai enzyme fermented with light of a specific wavelength. A characteristic crystalline structure of a combination of nutrients for promoting the regulation of the intestinal flora according to claim 1 and promoting the antioxidant and anti-inflammatory effects of the body. The yam bean enzyme and the acai enzyme fermented with light of a specific wavelength are characterized in that they are in the form of powder, which promotes the regulation of the intestinal flora according to claim 3 and also has an antioxidant effect and anti-antioxidant effect on the body. Crystal structure of a combination of nutrients to promote inflammatory effects. The combination of nutrients for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body further comprises probiotics, which are characterized by being powdery. The crystal structure of a combination of nutrients for promoting the regulation of the intestinal bacterial flora according to claim 1 and promoting the antioxidant and anti-inflammatory effects of the body. The spherical capsule is characterized by being a pectin or other edible colloid, in order to promote the regulation of the intestinal flora according to claim 1 and to promote the antioxidant action and anti-inflammatory action of the body. Crystal structure of a combination of nutrients. The intestinal tract according to claim 1, wherein the crystal structure of the combination of nutrients for promoting the regulation of the intestinal flora and promoting the antioxidant and anti-inflammatory effects of the body is translucent. Crystal structure of a combination of nutrients to promote the regulation of bacterial flora and promote the body's antioxidant and anti-inflammatory effects. The crystal structure of the nutritional combination to promote the regulation of the gut microbiota and promote the body's antioxidant and anti-inflammatory effects can be mixed with health products composed of drinks, colloids, or troches. The crystal structure of a nutritional combination for promoting the regulation of the intestinal flora according to claim 1 and promoting the antioxidant and anti-inflammatory effects of the body.

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