JP3227614B2 - Flow cell for liquid chromatography - Google Patents
Flow cell for liquid chromatographyInfo
- Publication number
- JP3227614B2 JP3227614B2 JP05423392A JP5423392A JP3227614B2 JP 3227614 B2 JP3227614 B2 JP 3227614B2 JP 05423392 A JP05423392 A JP 05423392A JP 5423392 A JP5423392 A JP 5423392A JP 3227614 B2 JP3227614 B2 JP 3227614B2
- Authority
- JP
- Japan
- Prior art keywords
- flow cell
- flow
- bypass
- pipe
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Optical Measuring Cells (AREA)
Description
【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION
【０００１】[0001]
【産業上の利用分野】本発明は、液体クロマトグラフ用
フローセルに関するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a flow cell for a liquid chromatograph.
【０００２】[0002]
【従来の技術】従来、液体クロマトグラフ用フローセル
は、検出器中に設置したフローセルにカラムと連結路に
て連結し、もう一方はドレインへの排出路を設けてあ
り、カラムを通った溶離液はフローセルを通りドレイン
に排出される如く構成してある。2. Description of the Related Art Conventionally, a flow cell for a liquid chromatograph is connected to a flow cell installed in a detector by a connecting line with a column, and the other is provided with a discharging line to a drain, and an eluent passed through the column Are configured to be discharged to the drain through the flow cell.
【０００３】この様な構成であるため、カラムを通過し
た溶離液は全てフローセルを通過することになる。[0003] With such a configuration, all eluents that have passed through the column pass through the flow cell.
【０００４】又、検出器のフローセルには、分析用、分
取用、大量分取用等使用する目的に応じ、使用するカラ
ムのサイズに対応して夫々を選択し、使用していた。Further, the flow cell of the detector is selected and used according to the size of the column to be used in accordance with the purpose to be used, such as for analysis, for preparative use, for large-scale preparative use.
【０００５】又、カラムサイズが大きくなるに従って、
流速が速くなるため、フローセルの配管径をカラムサイ
ズに応じて太いものに替える必要があった。Also, as the column size increases,
Since the flow rate becomes faster, it was necessary to change the pipe diameter of the flow cell to a larger one according to the column size.
【０００６】[0006]
【発明が解決しようとする課題】この結果、フローセル
の種類によって適応する流速が定まっているため、それ
以上の流速になると、配管径によって圧力が上昇して了
うので、フローセルにはその種類に応じて配管径を替え
なければならないが、その都度フローセルを交換するこ
とは極めて面倒な作業である。As a result, the flow velocity adapted to the type of the flow cell is determined. If the flow velocity becomes higher than that, the pressure is increased by the pipe diameter. The pipe diameter must be changed accordingly, but replacing the flow cell each time is extremely troublesome.
【０００７】[0007]
【課題を解決するための手段】 そこで本発明に於て
は、使用するカラムサイズが大きくなってもフローセル
の配管径を替える必要がなく、フローセルは一個であっ
ても、小流量から大流量まで対応することが出来るよう
にせんとするもので、検出器のフローセルの配管に、フ
ローセルに過剰の溶離液を流すことの出来るバイパスを
設け、且つフローセルとバイパスに流れる流量比を変え
ることにより、フローセルの適応流速範囲を広げること
の出来ることを特徴とする。Therefore, in the present invention, it is not necessary to change the pipe diameter of the flow cell even when the size of the column to be used becomes large. A bypass that allows excess eluent to flow through the flow cell is installed in the flow cell piping of the detector.
And change the flow ratio between the flow cell and bypass
The flow velocity range of the flow cell
And wherein the be possible.
【０００８】[0008]
【実施例】以下、図に示す一実施例により本発明を詳細
に説明する。１は溶離液容器で、該溶離液容器１に一端
を浸漬した連結管２は、ポンプ３を介してインジェクタ
ー４に連通し、更にカラム５を経て検出器６に連通して
ある。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail with reference to an embodiment shown in the drawings. Reference numeral 1 denotes an eluent container. A connecting pipe 2 having one end immersed in the eluent container 1 is connected to an injector 4 via a pump 3 and further to a detector 6 via a column 5.
【０００９】ポンプ３は無脈流ポンプ、シングルプラン
ジャーポンプ、ダブルプランジャーポンプ等から選択使
用し、連結管２にはダンパー付圧力計を使用するのがよ
い。As the pump 3, a non-pulsating pump, a single plunger pump, a double plunger pump or the like is selectively used, and a pressure gauge with a damper is preferably used for the connecting pipe 2.
【００１０】検出器６には目的に応じ、分光光度計、蛍
光光度計、ＲＩ検出器等を使用する。カラム５はその目
的に応じ、分析用、分取用を選択し、ガラスカラム、ス
テンレスカラム等用途により選択使用する。As the detector 6, a spectrophotometer, a fluorometer, an RI detector or the like is used according to the purpose. The column 5 is selected for analysis or preparative use according to the purpose, and is selectively used according to the purpose such as a glass column or a stainless steel column.
【００１１】７はフローセルで光路長や配管径を適宜選
択して、小流量から大流量まで対応できるようにしてお
くのがよい。該フローセル７の配管８には流入８１側と
流出側８２に夫々三方ジョイント９，１０を設け、両三
方ジョイント９，１０間にバイパス１１を設けてある。
このバイパス１１にはパイプを用い、そのパイプ径を所
望のものに取替え自在としておくのが便である。Reference numeral 7 denotes a flow cell which is suitably selected from a small flow rate to a large flow rate by appropriately selecting an optical path length and a pipe diameter. The pipe 8 of the flow cell 7 is provided with three-way joints 9 and 10 on the inflow 81 side and the outflow side 82, respectively, and a bypass 11 is provided between the three-way joints 9 and 10.
It is convenient to use a pipe for the bypass 11 and make the pipe diameter freely replaceable with a desired one.
【００１２】更に、このバイパス１１にはニードルバル
ブ等の流量調節器１２を設けることもできる（図２）。
又三方ジョイント９は配管８の流入側８１にのみ設け、
該三方ジョイント９に接続したバイパス１１は、流出側
８２に接続せずそのまゝドレインに流すごとくしておく
ことも出来る（図３）。Further, the bypass 11 may be provided with a flow controller 12 such as a needle valve (FIG. 2).
Also, the three-way joint 9 is provided only on the inflow side 81 of the pipe 8 ,
The bypass 11 connected to the three-way joint 9 can be left connected to the drain without being connected to the outflow side 82 (FIG. 3).
【００１３】次いで、その作動状態について説明する
と、ポンプ３によって溶離液は連結管２により送られ、
インジェクター４より注入される試料を伴ってカラム５
に送入される。こゝで試料の適正な解離を行ない、これ
を検出器６のフローセル７に導くのであるが、その際カ
ラム５を出た溶離液は三方ジョシント９に至ると一部は
フローセル７に、他はバイパス１１にながれる。この流
量比率は、フローセル７の配管８の流入側８１とバイパ
ス１１の管径によって定まる。又、バイパス１１にニー
ドルバルブ等の流量調節器１２を設置した場合、この調
節によって流量比率を所望のものを選択して設定するこ
とが出来る。Next, the operation state will be described. The eluent is sent by the connecting pipe 2 by the pump 3.
Column 5 with sample injected from injector 4
Will be sent to Here, the sample is appropriately dissociated and guided to the flow cell 7 of the detector 6. When the eluate exiting the column 5 reaches the three-way joint 9, a part of the eluate flows into the flow cell 7, and the other elutes. The flow goes to the bypass 11. This flow rate ratio is determined by the diameter of the inflow side 81 of the pipe 8 of the flow cell 7 and the diameter of the bypass 11. When a flow controller 12 such as a needle valve is installed in the bypass 11, a desired flow ratio can be selected and set by this adjustment.
【００１４】従来のカラムからの全溶離液をフローセル
に流す方式の場合、例えば光路長が２ｍｍ、配管径が
０．５ｍｍの分取用フローセルの場合、適応流速は１〜
３０ｍＬ／ｍｉｎ程度であり、これ以上の流速では配管
径によって圧力が上昇して了うが、同じ条件でも、バイ
パス１１に９、フローセル側に１流れるようにすると、
適応最大流速は３０＋２７０ｍＬ／ｍｉｎにすることが
出来る。即ち、バイパス１１の径を１．５ｍｍのものを
使用すればよいことになる。In the case of the conventional system in which all the eluent from the column flows through the flow cell, for example, in the case of a preparative flow cell having an optical path length of 2 mm and a pipe diameter of 0.5 mm, the adaptive flow rate is 1 to 5.
At a flow rate higher than about 30 mL / min, the pressure ends up rising depending on the pipe diameter. However, even under the same conditions, when 9 flows to the bypass 11 and 1 flows to the flow cell side,
The adaptive maximum flow rate can be 30 + 270 mL / min. That is, a bypass 11 having a diameter of 1.5 mm may be used.
【００１５】又、図３に示すフローセルの配管８の流入
側８１と流出側８２とを別々にして連結しない場合に
は、流出側８２よりの溶離液は排出して捨てて了うよう
にする。When the inflow side 81 and the outflow side 82 of the flow cell pipe 8 shown in FIG. 3 are not connected separately, the eluent from the outflow side 82 is discharged and discarded. .
【００１６】[0016]
【発明の効果】上記の如き本発明によれば、検出器のフ
ローセルの配管に、フローセルに過剰の溶離液を流すこ
との出来るバイパスを設け、且つフローセルとバイパス
に流れる流量比を変えることにより、フローセルの適応
流速範囲を広げることの出来るようにしたので、その、
バイパス比を替えてフローセルとバイパスの流量比を変
えることにより、適応流速範囲を広げることが出来、そ
の結果、フローセルを替えずに一つのフローセルによっ
て小流量から大流量に対応することが出来、実用上至便
である。According to the present invention as described above, a bypass through which excess eluent can flow into the flow cell is provided in the pipe of the flow cell of the detector , and the flow cell and the bypass are provided.
Flow cell adaptation by changing the flow ratio
Since the flow velocity range can be expanded ,
By changing the flow ratio between the flow cell and the bypass by changing the bypass ratio, the applicable flow velocity range can be expanded, and as a result, a single flow cell can handle small to large flow rates without changing the flow cell. It is very convenient.
【図１】 本発明一実施例概略説明図。FIG. 1 is a schematic explanatory view of one embodiment of the present invention.
【図２】 同上他実施例用部説明図。FIG. 2 is an explanatory view of a portion for another embodiment of the present invention.
【図３】 同上他実施例用部説明図。FIG. 3 is an explanatory view of a portion for another embodiment of the present invention.
１ 溶離液容器 ２ 連結管 ３ ポンプ ４ インジェクター ５ カラム ６ 検出器 ７ フローセル８ 配管 ９ 三方ジョイント １０ 三方ジョイント １１ バイパスDESCRIPTION OF SYMBOLS 1 Eluent container 2 Connecting pipe 3 Pump 4 Injector 5 Column 6 Detector 7 Flow cell 8 piping 9 Three-way joint 10 Three-way joint 11 Bypass
───────────────────────────────────────────────────── フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 30/64 G01N 27/28 321 ──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. 7 , DB name) G01N 30/64 G01N 27/28 321
Claims (3)
ルに過剰の溶離液を流すことの出来るバイパスを設け、
且つフローセルとバイパスに流れる流量比を変えること
により、フローセルの適応流速範囲を広げることの出来
る液体クロマトグラフ用フローセル。1. A bypass that allows excess eluent to flow through a flow cell is provided in a pipe of a flow cell of a detector ,
And changing the flow ratio between the flow cell and the bypass
Can extend the flow velocity range of the flow cell.
Liquid chromatographic flow cell that.
トを設け、該三方ジョイントに所望径のバイパスを取付
取外自在に設けたことを特徴とする液体クロマトグラフ
用フローセル。2. A flow cell for a liquid chromatograph, wherein a three-way joint is provided on the pipe inflow side of the flow cell, and a bypass having a desired diameter is provided on the three-way joint so as to be detachable.
パスにて連結させると共に、該バイパスに流量調節器を
設けたことを特徴とする液体クロマトグラフ用フローセ
ル。3. A flow cell for a liquid chromatograph, wherein a flow inflow side and an outflow side of a flow cell are connected by a bypass, and a flow controller is provided in the bypass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05423392A JP3227614B2 (en) | 1992-02-05 | 1992-02-05 | Flow cell for liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05423392A JP3227614B2 (en) | 1992-02-05 | 1992-02-05 | Flow cell for liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05215737A JPH05215737A (en) | 1993-08-24 |
| JP3227614B2 true JP3227614B2 (en) | 2001-11-12 |
Family
ID=12964826
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP05423392A Expired - Fee Related JP3227614B2 (en) | 1992-02-05 | 1992-02-05 | Flow cell for liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3227614B2 (en) |
-
1992
- 1992-02-05 JP JP05423392A patent/JP3227614B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH05215737A (en) | 1993-08-24 |
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