JP3217479U - Culture bag - Google Patents
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Abstract
【課題】細胞等を人工的な環境下で効率良く大量に培養するために、空気の交換率を向上することができる培養バッグを提供する。
【解決手段】細胞等を培養する培養バッグ１００であって、ガス透過性のある袋部材２１と、袋部材２１の表面であって、略円形の開口部２２とを有し、開口部２２は、袋部材２１の表面に所望の間隔で複数備える。袋部材２１の外枠を支持する外郭部材１１を更に有し、袋部材２１の外枠を支持して、袋部材２１の底面を平坦な状態で保持する。
【選択図】図１The present invention provides a culture bag capable of improving the air exchange rate in order to efficiently culture a large amount of cells and the like in an artificial environment.
A culture bag 100 for culturing cells and the like, which includes a gas permeable bag member 21, and a surface of the bag member 21, and a substantially circular opening 22. A plurality of surfaces are provided on the surface of the bag member 21 at desired intervals. The outer member 11 further supports the outer frame 11 of the bag member 21, supports the outer frame of the bag member 21, and holds the bottom surface of the bag member 21 in a flat state.
[Selection] Figure 1
Description
本考案は、細胞や組織の培養技術に関し、袋形状の部材を用いて細胞等を培養する培養バッグに関する。 The present invention relates to a cell and tissue culture technique, and relates to a culture bag for culturing cells and the like using a bag-shaped member.
医薬品の生産や遺伝子治療、再生医療、免疫療法等の分野において、細胞や組織、微生物、ウィルス等（以下、「細胞等」と言う）を人工的な環境下で効率良く大量に培養することが求められている。細胞等の培養では、ガス透過性のある袋体からなる培養バッグを用いることで、閉鎖系の培養システムを構築し易くすることができる。また、培養バッグでは、容量や培養面積を制御できると共に、細胞等及び培地を含む培養液の循環（送入排出）を自動化し易いなどのメリットがある。特許文献１には、培養液が充填された軟包材からなる培養バッグを載置して培養対象物の培養を行うための培養バッグ固定台であって、培養バッグを載置する架台を備え、前記架台の周縁部に二個以上の固定具が対向して立設され、前記固定具に凹部が形成されたことを特徴とする培養バッグ固定台に関する技術を開示している。特許文献２には、管軸方向が第１の方向に向けられるとともに前記第１の方向と交差する第２の方向に並置され且つ互いに隔壁で隔てられたガス透過性を有する複数の管状部と、前記複数の管状部のうち、互いに隣接する２つの管状部の各々を、前記管状部の一端と他端との間の中間領域において連通させるガス透過性を有する連通部と、を備える細胞培養バッグに関する技術を開示している。 In the fields of pharmaceutical production, gene therapy, regenerative medicine, immunotherapy, etc., cells, tissues, microorganisms, viruses, etc. (hereinafter referred to as “cells”) can be efficiently cultured in large quantities in an artificial environment. It has been demanded. In culturing cells or the like, it is possible to easily construct a closed culture system by using a culture bag made of a gas-permeable bag. In addition, the culture bag has advantages that the volume and the culture area can be controlled and that the circulation (feeding / discharging) of the culture solution containing cells and the culture medium can be easily automated. Patent Literature 1 is a culture bag fixing base for placing a culture bag made of a soft wrapping material filled with a culture medium and culturing a culture object, and includes a platform on which the culture bag is placed. A technique relating to a culture bag fixing table is disclosed, wherein two or more fixing tools are erected on the periphery of the gantry so as to face each other, and a recess is formed in the fixing tool. Patent Document 2 discloses a plurality of tubular portions having gas permeability, the tube axis direction being oriented in the first direction and juxtaposed in a second direction intersecting the first direction and separated from each other by a partition wall. A cell culture medium comprising: a gas-permeable communicating portion that communicates each of two adjacent tubular portions among the plurality of tubular portions in an intermediate region between one end and the other end of the tubular portion. Techniques relating to bags are disclosed.
しかしながら、特許文献１および特許文献２には、細胞等を人工的な環境下で効率良く大量に培養するために、空気の交換率を向上する方法の記載がない。 However, Patent Document 1 and Patent Document 2 do not describe a method for improving the air exchange rate in order to efficiently culture a large amount of cells and the like in an artificial environment.
本考案は、細胞等を人工的な環境下で効率良く大量に培養するために、空気の交換率を向上することができる培養バッグを提供することを目的とする。 An object of the present invention is to provide a culture bag capable of improving an air exchange rate in order to efficiently culture a large amount of cells and the like in an artificial environment.
上記課題を解決するために、本考案の一つの実施形態は、細胞等を培養する培養バッグであって、ガス透過性のある袋部材と、前記袋部材の表面であって、略円形の開口部とを有し、前記開口部は、前記袋部材の表面に所望の間隔で複数備える、ことを特徴とする培養バッグを提供する。 In order to solve the above problems, one embodiment of the present invention is a culture bag for culturing cells and the like, which is a gas-permeable bag member, a surface of the bag member, and a substantially circular opening. A culture bag is provided, wherein a plurality of the openings are provided at a desired interval on the surface of the bag member.
本考案の他の実施形態は、上記の培養バッグであって、前記開口部は、直径１０ｍｍから３０ｍｍである、ことを特徴とする培養バッグであってもよい。 Another embodiment of the present invention may be the culture bag described above, wherein the opening has a diameter of 10 mm to 30 mm.
本考案の他の実施形態は、上記の培養バッグであって、前記袋部材の外枠を支持する外郭部材を更に有し、前記外郭部材は、前記袋部材の外枠を支持して、該袋部材の底面を平坦な状態で保持する、ことを特徴とする培養バッグであってもよい。 Another embodiment of the present invention is the culture bag described above, further including an outer member that supports the outer frame of the bag member, the outer member supporting the outer frame of the bag member, The culture bag may be characterized in that the bottom surface of the bag member is held flat.
本考案に係る培養バッグによれば、細胞等を人工的な環境下で効率良く大量に培養するために、空気の交換率（供給機能）を向上することができる。 According to the culture bag according to the present invention, the air exchange rate (supply function) can be improved in order to efficiently culture a large amount of cells and the like in an artificial environment.
実施形態に係る培養バッグの例を用いて、本考案を説明する。なお、本考案は、以後に説明する培養バッグ以外でも、細胞等（細胞や組織、微生物、ウィルス等）を培養するために用いるガス透過性のある袋形状の部材（システム、装置、部品など）であれば、いずれのものにも用いることができる。なお、培養バッグの色、形状は、特に限定されない。培養バッグの色は、透明、半透明、色付きのものであってもよい。培養バッグの色は、例えば使用者（利用者）の使用方法に合わせた柄のものや広告、日付け、説明文などを記載したものであってもよい。また、培養バッグは、所望の模様や文字情報、メモ書きスペースなどが印刷されていてもよい。 The present invention will be described using an example of a culture bag according to an embodiment. In addition, the present invention is not limited to the culture bag described below, but a gas-permeable bag-shaped member (system, apparatus, component, etc.) used for culturing cells and the like (cells, tissues, microorganisms, viruses, etc.) If it is, it can be used for any. The color and shape of the culture bag are not particularly limited. The culture bag may be transparent, translucent or colored. The color of the culture bag may be, for example, a pattern according to the usage method of the user (user), an advertisement, a date, an explanatory note, or the like. The culture bag may be printed with a desired pattern, character information, a memo writing space, and the like.
以後に、本考案を説明する。 Hereinafter, the present invention will be described.
図１および図２を用いて、本考案の実施形態に係る培養バッグの構成を説明する。ここで、図１は、本考案の実施形態に係る培養バッグ１００の一例を説明する説明図である。図２は、本考案の実施形態に係る培養バッグ１００Ｕの一例を示す平面図である。なお、図１等に示す培養バッグの構成等は一例であり、本考案は図１等に示す培養バッグ等に限定されるものではない。 The configuration of the culture bag according to the embodiment of the present invention will be described with reference to FIGS. 1 and 2. Here, FIG. 1 is explanatory drawing explaining an example of the culture bag 100 which concerns on embodiment of this invention. FIG. 2 is a plan view showing an example of the culture bag 100U according to the embodiment of the present invention. In addition, the structure of the culture bag shown in FIG. 1 etc. is an example, and this invention is not limited to the culture bag etc. shown in FIG.
本考案に係る培養バッグは、細胞や組織の培養技術に関し、細胞等を内包して培養する袋形状の部材である。培養バッグは、培養する細胞等および培地を含む培養液を袋形状の内部に保持し、袋形状の内部で細胞等を培養する。本考案に係る培養バッグは、ガス透過性（たとえば酸素透過性及び二酸化炭素透過性）を有する袋形状の部材であれば、色、形状は特に限定されない。また、本考案に係る培養バッグは、細胞等の高い増殖効率を実現するために、低細胞毒性、低溶出性及び放射線滅菌適性を有することが好ましい。 The culture bag according to the present invention relates to a cell and tissue culture technique, and is a bag-shaped member that encapsulates cells and cultures. The culture bag holds cells to be cultured and a culture solution containing a medium inside the bag shape, and cultures the cells and the like inside the bag shape. The culture bag according to the present invention is not particularly limited in color and shape as long as it is a bag-shaped member having gas permeability (for example, oxygen permeability and carbon dioxide permeability). In addition, the culture bag according to the present invention preferably has low cytotoxicity, low elution property and suitability for radiation sterilization in order to realize high proliferation efficiency of cells and the like.
培養バッグは、２枚のプラスチックフィルムを貼り合せたもの、１枚のプラスチックフィルムを折り返して貼り合せたものであってもよい。プラスチックフィルムとしては、例えば、ポリエチレンやポリプロピレン、エチレンとα−オレフィンの共重合体、エチレンと酢酸ビニルの共重合体、エチレンとアクリル酸やメタクリル酸共重合体と金属イオンを用いたアイオノマー、ポリオレフィン、スチレン系エラストマー、ポリエステル系熱可塑性エラストマー、シリコーン系熱可塑性エラストマー、シリコーン樹脂等を用いることができる。また、培養バッグは、袋形状の内部で培養される細胞等の目視による観察を可能とするために、可視光を透過することが好ましい。 The culture bag may be one in which two plastic films are laminated and one plastic film is folded and pasted. Examples of the plastic film include polyethylene, polypropylene, ethylene / α-olefin copolymer, ethylene / vinyl acetate copolymer, ethylene / acrylic acid / methacrylic acid copolymer and ionomer using metal ions, polyolefin, Styrene-based elastomers, polyester-based thermoplastic elastomers, silicone-based thermoplastic elastomers, silicone resins, and the like can be used. In addition, the culture bag preferably transmits visible light in order to allow visual observation of cells or the like cultured inside the bag shape.
本考案に係る培養バッグは、細胞等を培養するものである。図１に示すように、本考案に係る培養バッグ１００は、ガス透過性のある袋部材２１と、袋部材２１の表面であって略円形の開口部２２とを有する。開口部２２は、本実施形態では、袋部材２１の表面に所望の間隔で複数備える。なお、また、所望の間隔は、設計、実験、計算などで予め定められる間隔とすることができる。 The culture bag according to the present invention is for culturing cells and the like. As shown in FIG. 1, a culture bag 100 according to the present invention has a gas permeable bag member 21 and a substantially circular opening 22 on the surface of the bag member 21. In the present embodiment, a plurality of openings 22 are provided on the surface of the bag member 21 at a desired interval. It should be noted that the desired interval can be set in advance by design, experiment, calculation, or the like.
図２に示すように、本考案に係る培養バッグ１００Ｕは、本実施形態では、例えば横２００ｍｍ〜２５０ｍｍ、縦３００ｍｍ〜４００ｍｍのものであってもよい。本考案に係る培養バッグは、例えば円形、楕円形、長方形、正方形、菱形、その他任意の形状であってもよい。なお、本考案に係る培養バッグの形状は、設計、実験、計算などで予め定められる形状とすることができる。 As shown in FIG. 2, the culture bag 100U according to the present invention may be, for example, a bag having a width of 200 mm to 250 mm and a length of 300 mm to 400 mm in the present embodiment. The culture bag according to the present invention may be, for example, circular, oval, rectangular, square, diamond, or any other shape. In addition, the shape of the culture bag according to the present invention can be a shape predetermined by design, experiment, calculation, or the like.
図２に示すように、本考案に係る培養バッグ１００Ｕの開口部は、直径１０ｍｍから３０ｍｍとすることができる。本考案に係る培養バッグの開口部の大きさは、設計、実験、計算などで予め定められる大きさとすることができる。また、本考案に係る培養バッグの開口部の数と配置は、設計、実験、計算などで予め定められる数と位置とすることができる。これにより、本考案に係る培養バッグ１００は、開口部から空気を交換（供給）することができるので、空気の交換率を向上することができる。 As shown in FIG. 2, the opening of the culture bag 100U according to the present invention can have a diameter of 10 mm to 30 mm. The size of the opening of the culture bag according to the present invention can be a size determined in advance by design, experiment, calculation, or the like. Moreover, the number and arrangement of the openings of the culture bag according to the present invention can be set to a predetermined number and position by design, experiment, calculation, and the like. Thereby, since the culture bag 100 which concerns on this invention can replace | exchange (supply) air from an opening part, it can improve the exchange rate of air.
図１に示すように、本考案に係る培養バッグ１００は、本実施形態では、袋部材２１の外枠を支持する外郭部材１１を更に有する。外郭部材１１は、袋部材２１の外枠を支持して、袋部材２１の底面を平坦な状態に維持することができる。これにより、本考案に係る培養バッグ１００は、外郭部材を用いて袋部材の底面を平坦な状態にして支持することができるので、平坦な培養面を得ることができ、細胞等および培養液の温度分布を容易に均一にすることができる。 As shown in FIG. 1, the culture bag 100 according to the present invention further includes an outer member 11 that supports the outer frame of the bag member 21 in the present embodiment. The outer member 11 can support the outer frame of the bag member 21 and maintain the bottom surface of the bag member 21 in a flat state. Thereby, since the culture bag 100 according to the present invention can be supported by using the outer shell member with the bottom surface of the bag member in a flat state, a flat culture surface can be obtained. The temperature distribution can be easily made uniform.
図１に示すように、本考案に係る培養バッグ１００は、本実施形態では、培養バッグ１００の位置を固定するときに用いる孔形状の固定部材１２と、培養液等を流入および流出（排出）する第１の流入流出部３１と第２の流入流出部３２を更に有する。本考案に係る培養バッグ１００は、たとえば固定部材１２の孔形状に固定器具を挿入して、培養バッグ１００の位置を固定することができる。 As shown in FIG. 1, a culture bag 100 according to the present invention, in this embodiment, has a hole-shaped fixing member 12 used when fixing the position of the culture bag 100, and inflows and outflows (discharge) of a culture solution and the like. The first inflow / outflow portion 31 and the second inflow / outflow portion 32 are further provided. The culture bag 100 according to the present invention can fix the position of the culture bag 100 by inserting a fixing device into the hole shape of the fixing member 12, for example.
これにより、本考案に係る培養バッグは、培養バッグを架台に載せて培養を行う場合と比較して（単に載せるだけでは培養バッグの底面にシワや弛みが生じて、平坦な培養面を得ることができない場合と比較して）、外郭部材を用いて袋部材の底面を平坦な状態にして支持することができ、固定部材を用いて培養バッグの位置を固定することができるので、細胞等が溜って過度に密集することを防止することができ、細胞等の移動や浮遊性を向上することができ、細胞等の増殖効率を向上することができる。また、本考案に係る培養バッグは、外郭部材を用いて袋部材の底面を平坦な状態にして支持することができるので、培養バッグ（袋部材）の底面を平坦な状態に維持することができ、培養面（袋部材）をシワや弛みのない状態にすることができ、培養面積を最大にすることができる。また、本考案に係る培養バッグは、外郭部材を用いて袋部材の底面を平坦な状態に維持することができるので、培養バッグ（袋部材）の内部で細胞等を均一に分布（分散）させることができ、細胞等の培養における培養条件の最適化を図ることができ、細胞等の増殖効率を一層向上させることができる。 As a result, the culture bag according to the present invention has a flat culture surface as compared with the case where the culture bag is placed on a gantry (if the culture bag is simply placed, the bottom surface of the culture bag is wrinkled or loosened). In comparison with the case where the cell cannot be used), the bottom of the bag member can be supported in a flat state using the outer member, and the position of the culture bag can be fixed using the fixing member. Accumulation and accumulation of cells can be prevented, the movement and floating of cells and the like can be improved, and the proliferation efficiency of cells and the like can be improved. In addition, since the culture bag according to the present invention can be supported by using the outer member with the bottom surface of the bag member in a flat state, the bottom surface of the culture bag (bag member) can be maintained in a flat state. The culture surface (bag member) can be free from wrinkles and slack, and the culture area can be maximized. Moreover, since the culture bag according to the present invention can maintain the bottom surface of the bag member in a flat state using the outer member, the cells and the like are uniformly distributed (dispersed) inside the culture bag (bag member). It is possible to optimize the culture conditions for culturing cells and the like, and the proliferation efficiency of cells and the like can be further improved.
以上のとおり、本考案に係る培養バッグによれば、外郭部材を用いて空気を交換（供給）することができるので、空気の交換率（供給機能）を向上することができ、細胞等を効率良く大量に培養することができる。また、本考案に係る培養バッグによれば、外郭部材を用いて袋部材の底面を平坦な状態にして支持することができるので、平坦な培養面を得ることができ、細胞等および培養液の温度分布を均一にすることができ、細胞等の増殖効率を向上させることができる。 As described above, according to the culture bag of the present invention, air can be exchanged (supplied) using the outer member, so that the air exchange rate (supply function) can be improved, and cells and the like can be efficiently used. It can be well cultured in large quantities. In addition, according to the culture bag according to the present invention, since the bottom surface of the bag member can be supported in a flat state using the outer member, a flat culture surface can be obtained, and the cells and the culture solution can be obtained. The temperature distribution can be made uniform, and the proliferation efficiency of cells and the like can be improved.
本考案に係る培養バッグで用いる培養液は、特に制限されず、あらゆる培養液を適用することができる。具体的には、哺乳動物細胞用の基本培地、幹細胞維持用培養液、昆虫細胞用の基本培地、酵母用培地、細菌用培地、等の液体培地である。また、培養液は、細胞を継続的に浮遊させる目的及び／又は細胞同士の過度の密着を防ぐ目的で、細胞毒性を有しない高分子化合物が添加されていてもよい。上記目的で培養液に添加される高分子化合物は、例えば、培養液の比重を調整する高分子化合物、培養液の粘度を調整する高分子化合物、培養液中で三次元ネットワーク構造を形成する高分子、等である。具体的には、高分子としては、セルロース、メチルセルロース、カルボキシメチルセルロース、ジェランガム、脱アシル化ジェランガム、ヒアルロン酸、アルギン酸、カラギーナン、キサンタンガム、ダイユータンガム、デンプン、ペクチン等の多糖類、コラーゲン、ゼラチン等のタンパク質、ポリエチレングリコール、ポリビニルピロリドン等の合成高分子などである。なお、培養液は、例えば、ペニシリン、ストレプトマイシン等の抗生物質；アスコルビン酸、レチノイン酸等のビタミン又はビタミン誘導体；グルコース等の糖源；アミノ酸；無機塩；血清、血清代替物；トランスフェリン等のタンパク質；インスリン等のホルモン；増殖因子；分化抑制因子；２−メルカプトエタノール、ジチオトレイトール等の抗酸化剤；カルシウムイオン、マグネシウムイオン、亜鉛イオン、鉄イオン、銅イオン等の金属イオン；などが添加されていてもよい。 The culture solution used in the culture bag according to the present invention is not particularly limited, and any culture solution can be applied. Specifically, a liquid medium such as a basic medium for mammalian cells, a culture solution for maintaining stem cells, a basic medium for insect cells, a medium for yeast, a medium for bacteria, and the like. In addition, a high molecular compound having no cytotoxicity may be added to the culture solution for the purpose of continuously floating cells and / or preventing excessive adhesion between cells. The polymer compound added to the culture solution for the above purpose includes, for example, a polymer compound that adjusts the specific gravity of the culture solution, a polymer compound that adjusts the viscosity of the culture solution, and a high molecular compound that forms a three-dimensional network structure in the culture solution. Molecules, etc. Specifically, examples of the polymer include cellulose, methylcellulose, carboxymethylcellulose, gellan gum, deacylated gellan gum, hyaluronic acid, alginic acid, carrageenan, xanthan gum, diutane gum, starch, pectin and other polysaccharides, collagen, gelatin and the like. And synthetic polymers such as protein, polyethylene glycol, and polyvinylpyrrolidone. The culture solution is, for example, an antibiotic such as penicillin or streptomycin; a vitamin or vitamin derivative such as ascorbic acid or retinoic acid; a sugar source such as glucose; an amino acid; an inorganic salt; a serum or a serum substitute; a protein such as transferrin; Hormones such as insulin; growth factors; differentiation inhibitors; antioxidants such as 2-mercaptoethanol and dithiothreitol; metal ions such as calcium ions, magnesium ions, zinc ions, iron ions and copper ions; May be.
本考案に係る培養バッグにおいて、培養の対象となる細胞は、特に制限されず、幹細胞、動物細胞、植物細胞、真菌細胞、細菌細胞、プロトプラスト、樹立された細胞株、人為的に遺伝子改変が施された細胞、等のあらゆる細胞が対象となり得る。幹細胞は、自己複製能と分化能とを有する細胞であれば特に制限されず、多能性幹細胞でもよく、体性幹細胞でもよい。多能性幹細胞は、自己複製能と、外胚葉、中胚葉および内胚葉のいずれにも分化し得る多分化能とを有する細胞である。多能性幹細胞としては、胚性幹細胞、人工多能性幹細胞、胚性生殖細胞、胚性癌細胞、多能性成体前駆細胞、成体多能性幹細胞、Ｍｕｓｅ細胞などが挙げられる。体性幹細胞としては、例えば、間葉系幹細胞、造血幹細胞、神経幹細胞などが挙げられる。 In the culture bag according to the present invention, cells to be cultured are not particularly limited, and stem cells, animal cells, plant cells, fungal cells, bacterial cells, protoplasts, established cell lines, artificially genetically modified. Any cell can be targeted, such as a treated cell. The stem cells are not particularly limited as long as they have self-replicating ability and differentiation ability, and may be pluripotent stem cells or somatic stem cells. A pluripotent stem cell is a cell having self-renewal ability and pluripotency capable of differentiating into any of ectoderm, mesoderm and endoderm. Examples of pluripotent stem cells include embryonic stem cells, induced pluripotent stem cells, embryonic germ cells, embryonic cancer cells, pluripotent adult progenitor cells, adult pluripotent stem cells, and Muse cells. Examples of somatic stem cells include mesenchymal stem cells, hematopoietic stem cells, neural stem cells and the like.
培養対象となる細胞の他の例は、生体を構成する体細胞である。具体的には、リンパ球、好中球、単球、巨核球、マクロファージ、線維芽細胞、基底細胞、ケラチノサイト、上皮前駆細胞、周皮細胞、内皮細胞、脂肪前駆細胞、筋芽細胞、骨芽細胞、軟骨細胞、肝実質細胞、膵β細胞、グリア細胞、等が挙げられる。 Another example of cells to be cultured is a somatic cell constituting a living body. Specifically, lymphocytes, neutrophils, monocytes, megakaryocytes, macrophages, fibroblasts, basal cells, keratinocytes, epithelial progenitor cells, pericytes, endothelial cells, adipose precursor cells, myoblasts, osteoblasts Cells, chondrocytes, hepatocytes, pancreatic β cells, glial cells, and the like.
本考案は、閉鎖系で細胞を大量に培養する遺伝子治療、免疫療法、再生医療や抗体医薬生産などにおいて好適に利用することが可能である。 The present invention can be suitably used in gene therapy, immunotherapy, regenerative medicine, antibody drug production, etc., in which cells are cultured in large quantities in a closed system.
以上のとおり、本考案に係る実施形態について説明したが、本考案は上記の実施形態に限定されるものではない。すなわち、本考案は、実用新案登録請求の範囲に記載の内容に基づいて、様々に変形、変更又はその他任意に改変され得る。 As mentioned above, although embodiment which concerns on this invention was described, this invention is not limited to said embodiment. That is, the present invention can be variously modified, changed, or arbitrarily modified based on the contents described in the claims for utility model registration.
１００，１００Ｕ ： 培養バッグ
１１ ： 外郭部材
１２ ： 固定部材
２１ ： 袋部材
２２ ： 開口部
３１ ： 第１の流入流出部
３２ ： 第２の流入流出部
100, 100U: Culture bag 11: Outer member 12: Fixing member 21: Bag member 22: Opening portion 31: First inflow / outflow portion 32: Second inflow / outflow portion
Claims (3)
ガス透過性のある袋部材と、
前記袋部材の表面であって、略円形の開口部と
を有し、
前記開口部は、前記袋部材の表面に所望の間隔で複数備える、
ことを特徴とする培養バッグ。 A culture bag for culturing cells and the like,
A gas permeable bag member;
A surface of the bag member having a substantially circular opening;
A plurality of the openings are provided at a desired interval on the surface of the bag member,
A culture bag characterized by that.
前記外郭部材は、前記袋部材の外枠を支持して、該袋部材の底面を平坦な状態で保持する、
ことを特徴とする、請求項１または請求項２に記載の培養バッグ。 An outer member for supporting the outer frame of the bag member;
The outer member supports the outer frame of the bag member and holds the bottom surface of the bag member in a flat state.
The culture bag according to claim 1 or 2, characterized in that.
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