JP3183678U - Electrochemical detection sheet with blood filtration function - Google Patents

Abstract

Disclosed is an electrochemical detection sheet having a blood filtration function, which achieves blood filtration and exhaust path formation functions by laminating a multilayer porous filtration membrane and a hydrophilic layer.
An electrochemical detection sheet having a blood filtration function includes a first substrate 10, an electrode system 11, a reaction pad 12, a second substrate 13, at least one filtration membrane 14, a hydrophilic layer 15, a third substrate 16, and a first substrate. A fourth substrate 17 and a fifth substrate 18.
[Selection] Figure 1A

Description

  The present invention relates to an electrochemical detection sheet, and more particularly, to an electrochemical detection sheet having a disposable blood filtration and exhaust function.

With the advance of medical care, the average life span of human beings is gradually increasing, while various civilization diseases are also universally present in society.
According to a report promulgated by the World Health Organization (WHO), non-communicable diseases such as cancer and cardiovascular disease are the main threats to human health. Seven million people have died of cardiovascular disease. In 2030, the number of people who die from cardiovascular diseases is expected to reach 25 million worldwide. In addition, according to the statistics of the Japanese health office, there are approximately 1.45 million heart disease patients in the country.

Previous studies have shown a close relationship between cholesterol and cardiovascular disease. Any increase or decrease in the cholesterol index in the blood has considerable significance for the prevention or control of the disease.
So far, methods for testing blood cholesterol include “optical quantification method” and “chromatography quantification method (stratification)”, both of which require a long test time, and the former is interference of the shielding effect of hemoglobin. The latter was semi-quantitative, the operation method was complicated, and the determination of the results was difficult.

  With the spread of home inspection equipment, detection sheets corresponding to various types have been developed, and among them, cholesterol detection sheets are one of the main products. Up to now, the principle of electrochemical analysis and the method of filtering red blood cells with filter paper have been combined, but the structure is complicated. In addition, the process is complicated, and blood cannot be efficiently transported to the designated reaction area unless an exhaust pipe is provided for the process of red blood cell filtration using filter paper. There was a need to increase the cost. Therefore, a detection sheet having a blood filtration and exhaust function and a simplified structure has been a problem to be solved by those skilled in the art.

Therefore, the main object of the present invention is an electrochemical detection sheet having a blood filtration function, which achieves the function of blood filtration and exhaust passage formation by laminating a multilayer porous filtration membrane and a hydrophilic layer. Is to provide a sheet.
When blood passes through the filtration membrane and the hydrophilic layer, blood cells are filtered, and gas (gas) is exhausted from the holes formed on the left and right sides.

  Another object of the present invention is an electrochemical detection sheet having a blood filtration function, in which a sample having a specific function is applied to a filtration membrane, and the sample undergoes a biochemical reaction with a non-measurement object in the sample. Thus, an electrochemical detection sheet that achieves a screening function and detects low density lipoprotein cholesterol (LDL) and high density lipoprotein cholesterol (HDL) is provided.

  Another object of the present invention is an electrochemical detection sheet having a blood filtration function, which has a function of sandwiching the front end of the sheet with a jig and fixing the detection sheet with the jig to absorb a predetermined blood volume. It is to provide an electrochemical detection sheet.

  Another object of the present invention is to provide an electrochemical detection sheet having a blood filtration function, and to provide a sheet coated with a reaction sample having specificity to the reaction pad, such as a cholesterol reaction sample. Due to its structure, when a reaction sample with specificity is applied, the contact frequency between the reaction sample and the measurement object increases, and an electrochemical detection sheet having a blood filtration function capable of enhancing the reaction signal is provided. That is.

  Still another object of the present invention is an electrochemical detection sheet having a blood filtration function, which achieves an exhausting action by laminating a multilayer porous filtration membrane and a hydrophilic layer, and separately providing an exhaust passage. Since there is no need, it is providing the electrochemical detection sheet | seat with the blood filtration function which can reduce manufacturing cost.

  In view of the above object, the present invention is an electrochemical detection sheet having a blood filtration function, the first substrate having a first surface and a second surface facing the first surface, and the first substrate An electrode system having at least a working electrode and a reference electrode, and a sample formed by applying at least one reaction sample and filtering and passing a measured fluid through the electrode system; A reaction pad that produces a biochemical reaction upon contact, a second substrate that covers the first substrate, the electrode system, and the reaction pad surface and has a first opening that exposes the reaction pad; and a first of the second substrate A filtration membrane that is above the opening and covers the reaction pad and filters the measurement fluid to make the measurement fluid a sample, a hydrophilic layer that covers the filtration membrane and filters the measurement fluid, and covers the hydrophilic layer , Position of the first opening A corresponding third substrate having a second opening that exposes the hydrophilic layer and fixes the hydrophilic layer, the filtration membrane, and the reaction pad in the same position, covers the upper side of the third substrate, and covers the second opening on the side surface of the third substrate. And a fourth substrate having an inner slit through which a measurement fluid can enter, and a fifth substrate covering the fourth substrate.

  Next, the present invention is an electrochemical detection sheet having a blood filtration function, the first substrate having a first surface and a second surface facing the first surface, and the first surface of the first substrate An electrode system having at least a working electrode and a reference electrode, and covering the electrode system and applying at least one reaction sample, and contacting with the sample formed by passing the measured fluid and being filtered A reaction pad that generates a chemical reaction; a second substrate that covers the surface of the first substrate, the electrode system, and the reaction pad; and has a first opening that exposes the reaction pad; and at least one upper filtration membrane and lower filtration membrane. The lower filtration membrane covers the reaction pad and the measurement fluid is filtered to use the measurement fluid as a sample; the upper filtration membrane covers the lower filtration membrane and the measurement fluid is filtered; and the hydrophilic layer is covered. The second opening at the position corresponding to the first opening A third substrate that exposes the hydrophilic layer and fixes the hydrophilic layer, the filtration membrane, and the reaction pad on the same position, covers the upper side of the third substrate, and corresponds to the second opening on the side surface of the third substrate. There is provided an electrochemical detection sheet having a blood filtration function, comprising a fourth substrate having an inner slit so that a measurement fluid can enter a position, and a fifth substrate covering the fourth substrate.

The present invention has the following features.
1. The detection sheet has a blood filtration function.
2. The detection sheet has a screening function and can detect low density lipoprotein cholesterol or high density lipoprotein cholesterol.
3. The detection sheet detects blood total cholesterol.
4). The detection sheet has an exhaust function.
5. The exhaust action of the detection sheet is naturally formed, and it is not necessary to provide a separate exhaust passage, thereby reducing the cost.

  In order to clarify the objects, features and embodiments of the present invention, the drawings will be described as follows.

The perspective view which shows each member of the electrochemical detection sheet | seat which has the blood filtration function which is the 1st Example of this invention. BRIEF DESCRIPTION OF THE DRAWINGS The top view which shows the whole electrochemical detection sheet | seat with a blood filtration function which is a 1st Example of this invention. Sectional drawing which shows filtration and screening of the measurement fluid of the electrochemical detection sheet | seat with a blood filtration function of this invention. The perspective view which shows each member of the electrochemical detection sheet | seat which has the blood filtration function which is the 2nd Example of this invention. The top view which shows the whole electrochemical detection sheet | seat with a blood filtration function which is a 2nd Example of this invention. The perspective view which shows each member of the electrochemical detection sheet | seat with a blood filtration function which is the 3rd Example of this invention. The top view which shows the whole electrochemical detection sheet | seat with a blood filtration function which is the 3rd Example of this invention. Sectional drawing which shows filtration and screening of the measurement fluid of the electrochemical detection sheet | seat which has the blood filtration function which is the 3rd Example of this invention.

  The embodiments of the present invention disclosed herein are examples of the present invention, and may be implemented in other equivalent modifications. Further, the drawings referred to below of the present invention show schematic diagrams related to the features of the present invention, and provide supplementary explanations of better embodiments of the present invention, and the scope of rights to which the present invention intends to protect. Is based on the scope of the subsequent utility model registration request.

The present invention is an electrochemical detection sheet having a blood filtration function, and the detection sheet naturally forms a blood filtration function and an exhaust function by laminating a multilayer porous filtration membrane and a hydrophilic hydrophilic layer. It is characterized by doing.
In addition, by applying the sample or reaction sample on a filtration membrane or reaction pad, the detection sheet has a screening function, and further detects low density lipoprotein cholesterol, high density lipoprotein cholesterol, or total cholesterol. In addition, there is no need to provide a separate exhaust path, and the cost can be reduced.

  With respect to the above technical contents, features, and effects, a more preferred embodiment of the present invention will be clearly described below with reference to the drawings.

  The meanings of the terms used in the present specification are clarified by the following definitions.

  “Gas escape”, “exhaust action” or similar terms mean that the measurement fluid enters the inlet of the electrochemical detection sheet and is introduced into the porous filtration membrane or hydrophilic layer of each layer by capillary action to reach the reaction region. Then, since the filtration membrane and the hydrophilic membrane are porous materials, the layers do not stick to each other, and excess gas in the measurement fluid escapes from the gaps between the layers and the gaps between the layers. It refers to quickly absorbing the sample and not affecting the accuracy of detection.

  The term “measuring fluid” or similar term refers herein to the detection of whole blood or blood that has not been removed.

  The term “sample” or similar term here refers to blood that has undergone a biochemical reaction with a reaction sample with blood from which non-measurement has been removed. Sample components include low density lipoprotein cholesterol, high density lipoprotein cholesterol, or total cholesterol.

  "Reference electrode" or similar term here refers to an electrode having an electrode potential that is stable and reproducible. Using this as a reference, the potentials of the other electrodes are measured. An electrode that provides a standard potential and is independent of changes in composition or concentration of the measurement system. Commonly used reference electrodes are hydrogen electrode, calomel electrode (SHE), Ag / AgCl, and Hg / Hg2Cl2 electrode, and calomel electrode is most often used.

  The term “working electrode” or similar term here refers to an electrode that shows a clear change in the concentration of the main body of the sample when a larger current passes through the detection process of the electrochemical detection sheet, and this electrode is the cathode or anode. It is not limited. Looking at the reaction caused by this electrode, the anode is when the electrode undergoes an oxidation reaction, and the cathode when the electrode undergoes a reduction reaction.

  1A to 1C show an electrochemical detection sheet 2 having a blood filtration function according to a first embodiment of the present invention. FIG. 1A shows that an electrochemical detection sheet 2 having a blood filtration function includes a first substrate 10, an electrode system 11, a reaction pad 12, a second substrate 13, at least one filtration membrane 14, a hydrophilic layer 15, a third substrate 16, It shows that the fourth substrate 17 and the fifth substrate 18 are included.

The first substrate 10 includes a first surface 101, a second surface 102 facing the first surface 101, and an electrode system 11 located on the first surface 101. The electrode system 11 includes a working electrode 111 and a reference electrode. 112 is included.
The upper layer of the electrode system 11 is made of a material such as glass fiber or cotton fiber, and is covered with a reaction pad 12 having a thickness in the range of 0.1 to 0.3 mm and a pore diameter of 0.2 to 1 μm. Since the reaction pad 12 has a three-dimensional structure, when a specific reaction sample is applied, the contact probability between the reaction sample and the measured sample is increased, and the reaction signal is enhanced.
The position where the reaction pad 12 is placed is called a reaction area. The reaction pad 12 contains at least one reaction sample and causes a biochemical reaction in contact with the sample. The reaction sample used in this example is used for cholesterol action.

The second substrate 13 covers the surfaces of the first substrate 10, the electrode system 11, and the reaction pad 12, and a first opening 131 is provided on the second substrate 13 to expose the reaction pad 12 in the reaction region. That is, the size of the first opening 131 is a size that can expose most of the region of the reaction pad 12.
The reaction pad 12 is covered with at least one filtration membrane 14. The filter membrane 14 filters the measurement fluid and uses it as a sample. The material of the filtration membrane 14 is glass fiber or cotton fiber, the thickness is in the range of 0.3 to 0.5 mm, and the pore diameter is in the range of 0.5 to 2 μm. The hydrophilic layer 15 covers the filtration membrane 14 and filters the measurement fluid.

The third substrate 16 covers the hydrophilic layer 15 to fix the positions of the hydrophilic layer 15, the filtration membrane 14, and the reaction pad 12 above the reaction region, and the third substrate 16 is positioned at a position corresponding to the first opening 131. The hydrophilic layer 15 is exposed with two openings 161.
The fourth substrate 17 is covered above the third substrate 16 and has one inner slit 171 at the edge. The position of the inner slit 171 corresponds to the second opening 161 and serves as an inlet for the measurement fluid, and has a function of quantifying the volume of the measurement fluid.
The fifth substrate 18 is covered above the fourth substrate 17, and the whole becomes the electrochemical detection sheet 2 having a blood filtration function as shown in FIG. 1B.

FIG. 1C is a cross-sectional view of the electrochemical detection sheet 2 having a blood filtration function drawn along the cross-sectional line AA of FIG. 1B, and in order from the bottom to the top, the first substrate 10, the reaction pad 12, and the second substrate. 13 shows a filtration membrane 14, a hydrophilic layer 15, a third substrate 16, a fourth substrate 17, and a fifth substrate 18.
A measurement fluid (not shown) enters the sheet from the indented slit 171 of the fourth substrate 17, guides the measurement fluid by capillary action, moves it to the inside of the sheet along the direction of W 1, and reaches the second opening 161. When it reaches, it moves down along the direction of W2. The measurement fluid is filtered through each layer, reaches the reaction pad 12 as a sample, contacts the reaction sample, and causes a biochemical reaction.
In the filtration process, since the filtration membrane 14, the hydrophilic layer 15 and the reaction pad 12 are porous materials, if the measurement fluid moves along the W2 direction without sealing each layer, excess gas in the measurement fluid Escapes from the gaps between the layers and the gaps between the layers, and naturally produces an exhaust action.

  2A and 2B show an electrochemical detection sheet 3 having a blood filtration function, which is a second embodiment of the present invention, and includes at least one of a first substrate 30, an electrode system 31, a reaction pad 32, and a second substrate 33. Filter membrane 34, hydrophilic layer 35, third substrate 36, fourth substrate 37, fifth substrate 38 and fixed scissors 39.

Referring to FIG. 2A, the arrangement method of each member of the electrochemical detection sheet 3 having the blood filtration function of the present embodiment is the same as that of the first embodiment, and the first substrate 30 includes a first surface 301, A second surface 302 facing the first surface 301 and an electrode system 31 located on the first surface 301 are included, and the electrode system 31 includes a working electrode 311 and a reference electrode 312.
The upper layer of the electrode system 31 is made of a material such as glass fiber or cotton fiber, and is covered with a reaction pad 32 having a thickness in the range of 0.1 to 0.3 mm and a pore diameter of 0.2 to 1 μm. Since the reaction pad 32 has a three-dimensional structure, when a specific reaction sample is applied, the probability of contact between the reaction sample and the sample to be measured is increased, and the reaction signal is enhanced. The position where the reaction pad 32 is placed is referred to as a reaction area.
The reaction pad 32 contains at least one reaction sample, and comes into contact with one sample to cause a biochemical reaction. The reaction sample used in this example is used for cholesterol action.

The second substrate 33 covers the surfaces of the first substrate 30, the electrode system 31, and the reaction pad 32, and a first opening 331 is provided on the second substrate 33 to expose the reaction pad 32 in the reaction region. That is, the size of the first opening 331 is a size that can expose most of the region of the reaction pad 32.
The reaction pad 32 is covered with at least one filtration membrane 34. The filter membrane 34 filters the measurement fluid and uses it as a sample. The material of the filtration membrane 34 is glass fiber or cotton fiber, the thickness is in the range of 0.3 to 0.5 mm, and the pore diameter is in the range of 0.5 to 2 μm. The hydrophilic layer 35 covers the filter membrane 34 and filters the measurement fluid.

The third substrate 36 covers the hydrophilic layer 35 and fixes the positions of the hydrophilic layer 35, the filtration membrane 34, and the reaction pad 32 above the reaction region. The third substrate 36 has a second opening 361 at a position corresponding to the first opening 331 to expose the hydrophilic layer 35.
The fourth substrate 37 is covered above the third substrate 36, and an inner slit 371 is provided at a position corresponding to the second opening 361 on the edge thereof, serving as an inlet for the measurement fluid, and having a function of quantifying the volume of the measurement fluid. A fifth substrate 38 covers the fourth substrate 37.

In addition, the fixing scissors 39 are fitted on the electrochemical detection sheet 3 having a blood filtration function. The fixing scissors 39 include a flat surface 391 and a plurality of fixing claws 392 protruding upward from the four corners of the flat surface 391.
At the same time that the flat surface 391 and the second surface 302 of the first substrate 30 are in contact, the plurality of fixing claws 392 are locked to the fifth substrate 38 and the fixing scissors 39 have a blood filtration function. Cover. The fixed scissors 39 can set the volume of the inhaled measurement fluid to a predetermined fixed value. Therefore, the entire electrochemical detection sheet 3 having a blood filtration function is as shown in FIG. 2B.

  3A to 3C, an electrochemical detection sheet 5 having a blood filtration function according to a third embodiment of the present invention includes a first substrate 40, an electrode system 41, a reaction pad 42, a second substrate 43, A filtration membrane system 44, a hydrophilic layer 45, a third substrate 46, a fourth substrate 47 and a fifth substrate 48 are provided.

The first substrate 40 includes a first surface 401, a second surface 402 facing the first surface 401, an electrode system 41 including a working electrode 411 and a reference electrode 412 positioned on the first surface 401, and an upper layer of the electrode system 41. The reaction pad 42 is made of glass fiber or cotton fiber covering the surface, and has a thickness in the range of 0.1 to 0.3 mm and a pore diameter in the range of 20 to 100 μm.
Since the reaction pad 42 has a three-dimensional structure, when a reaction sample having specificity is applied, the probability of collision between the reaction sample and the sample increases, thereby enhancing the reaction signal. The position where the reaction pad 42 is placed is referred to as a reaction area.
At least one reaction sample is contained in the reaction pad 42 and contacts the sample to cause a biochemical reaction. The reaction sample in this example is used for cholesterol action.

The second substrate 43 covers the surfaces of the first substrate 40, the electrode system 41, and the reaction pad 42, and the second substrate 43 has a first opening 431 to expose the reaction pad 42 in the reaction region. That is, the size of the first opening 431 is a size that allows most of the reaction pad 42 to be exposed. A filtration membrane system 44 covers the reaction pad 42, and the measurement fluid is filtered to obtain the sample.
The filtration membrane system 44 includes at least an upper filtration membrane 442 and a lower filtration membrane 441, which covers the reaction pad 42 and optionally applies a first sample. The material of the lower filtration membrane 441 is glass fiber or cotton fiber, the thickness is in the range of 0.3 to 0.5 mm, and the pore diameter is in the range of 50 to 200 μm.
The upper filtration membrane 442 covers the lower filtration membrane 441 and applies the second sample. The material of the upper filtration membrane 442 is glass fiber or cotton fiber, the thickness is in the range of 0.1 to 0.5 mm, and the pore diameter is in the range of 10 to 30 μm. The hydrophilic layer 45 covers the upper filtration membrane 442 and filters the measurement fluid.

The third substrate 46 covers the hydrophilic layer 45 and fixes the positions of the hydrophilic layer 45, the filtration membrane system 44, and the reaction pad 42 above the reaction region.
A second opening 461 is provided at a position corresponding to the first opening 431 of the third substrate 46 to expose the hydrophilic layer 45. The fourth substrate 47 is covered above the third substrate 46, and an inner slit 471 is provided at a position corresponding to the second opening 461 on the edge thereof, which serves as an inlet for the measurement fluid and has a function of quantifying the volume of the measurement fluid.
The fifth substrate 48 covers the upper side of the fourth substrate 47, and the whole becomes the electrochemical detection sheet 5 having a blood filtration function as shown in FIG. 3B.

FIG. 3C is a cross-sectional view of the electrochemical detection sheet 5 having a blood filtration function drawn along a cross-sectional line BB in FIG. 3B, and sequentially from the bottom to the top, the first substrate 40, the reaction pad 42, and the second substrate. 43, a filtration membrane system 44 (including a lower filtration membrane 441 and an upper filtration membrane 442), a hydrophilic layer 45, a third substrate 46, a fourth substrate 47, and a fifth substrate 48.
The measurement fluid (not shown) enters the sheet from the inner slit 471 of the fourth substrate 47, guides the measurement fluid by capillary action, moves to the inside of the sheet along the direction of W3, and reaches the second opening 461. Then, it moves downward along the W4 direction. The measurement fluid is filtered through each layer to become a sample, reaches the reaction pad 42, and comes into contact with the reaction to cause a biochemical reaction.
In the process of filtration, the filtration membrane system 44, the hydrophilic layer 45 and the reaction pad 42 are porous materials, and without sealing between the layers, excess gas in the measurement fluid is generated between the pores between the layers and the layers. Escape from the gap between the two, and naturally exhaust.

  In addition, the electrochemical detection sheet 5 having the blood filtration function of the present embodiment may be provided with a fixing scissors 39 as shown in FIGS. 2A and 2B as necessary. The fixing scissors 39 are fitted on the electrochemical detection sheet 5 having a blood filtration function, and fix the volume of the absorbed measurement fluid.

  When the electrochemical detection sheet 5 having a blood filtration function according to the third embodiment of the present invention is used as a detection sheet for high density lipoprotein cholesterol (HDL), it is not necessary to apply the first sample to the lower filtration membrane 441. Then, it is applied to the upper filtration membrane 442 to obtain a surface active sample when non-high density lipoprotein cholesterol (non-HDL) is precipitated.

  When the electrochemical detection sheet 5 having a blood filtration function according to the third embodiment of the present invention is used as a low density lipoprotein cholesterol (LDL) detection sheet, the lower filtration membrane 441 has specificity for low density lipoprotein cholesterol. Apply a surface active sample to develop the protein structure of low density lipoprotein cholesterol. The upper filtration membrane 442 is coated with a surface active sample that surrounds non-low density lipoprotein cholesterol (non-LDL).

The characteristics of the electrochemical detection sheet having the blood filtration function of the present invention are as follows.
1. The detection sheet has a blood filtration function.
2. The detection sheet has a screening function and can detect low density lipoprotein cholesterol or high density lipoprotein cholesterol.
3. The detection sheet detects blood total cholesterol.
4). The detection sheet has an exhaust function.
5. The exhaust action of the detection sheet is naturally formed, and it is not necessary to provide a separate exhaust passage, thereby reducing the cost.

  As mentioned above, although the Example of this invention was explained in full detail with reference to drawings, the electrochemical detection sheet | seat with the blood filtration function of this invention is not restricted to this Example, and does not deviate from the summary of this invention. Range design changes and the like are also included in the present invention.

Electrochemical detection sheet with blood filtration function 2, 3, 5
First substrate 10, 30, 40
First surface 101, 301, 401
Second surface 102, 302, 402
Electrode system 11, 31, 41
Working electrode 111, 311, 411
Reference electrode 112, 312, 412
Reaction pad 12, 32, 42
Second substrate 13, 33, 43
First opening 131, 331, 431
Filter membrane 14, 34
Filtration membrane system 44
Upper filtration membrane 442
Lower filtration membrane 441
Hydrophilic layer 15, 35, 45
Third substrate 16, 36, 46
Second opening 161, 361, 461
Fourth substrate 17, 37, 47
Inner slit 171, 371, 471
Fifth substrate 18, 38, 48
Fixed scissors 39
Plane 391
Fixed claw 392
Measuring fluid direction W1, W2, W3, W4

Claims (2)

An electrochemical detection sheet having a blood filtration function,
A first substrate having a first surface and a second surface facing the first surface;
An electrode system located on the first surface of the first substrate and having at least a working electrode and a reference electrode;
A reaction pad that covers the electrode system and applies at least one reaction sample and causes a biochemical reaction upon contact with the sample through which the measured fluid passes and is filtered;
A second substrate covering the first substrate, the electrode system and the reaction pad surface and having a first opening exposing the reaction pad;
A filtration membrane above the first opening of the second substrate and covering the reaction pad, filtering the measurement fluid and using the measurement fluid as a sample;
A hydrophilic layer that covers the top of the filtration membrane and filters the measurement fluid;
A third substrate covering the hydrophilic layer and having a second opening corresponding to the position of the first opening, exposing the hydrophilic layer and fixing the hydrophilic layer, the filtration membrane and the reaction pad in the same position;
A fourth substrate that covers an upper side of the third substrate and has an inner slit corresponding to the position of the second opening on one side surface of the third substrate, and a fifth substrate that covers the fourth substrate; Characterized by comprising
Electrochemical detection sheet with blood filtration function. An electrochemical detection sheet having a blood filtration function,
A first substrate having a first surface and a second surface facing the first surface;
An electrode system located on the first surface of the first substrate and having at least a working electrode and a reference electrode;
A reaction pad that covers the electrode system and applies at least one reaction sample and causes a biochemical reaction upon contact with the sample through which the measured fluid passes and is filtered;
A second substrate covering the first substrate, the electrode system and the reaction pad surface and having a first opening exposing the reaction pad;
It has at least an upper filtration membrane and a lower filtration membrane, the lower filtration membrane covers a reaction pad and filters the measurement fluid to make a sample, and the upper filtration membrane covers the lower filtration membrane and filters the measurement fluid A filtration membrane system;
A hydrophilic layer that covers the top of the filtration membrane and filters the measurement fluid;
A third covering the hydrophilic layer, having a second opening at a position corresponding to the first opening, exposing the hydrophilic layer, and fixing the hydrophilic layer, the filtration membrane, and the reaction pad on the same position A substrate,
A fourth substrate having an inner slit that covers the upper side of the third substrate and allows the measurement fluid located corresponding to the second opening to enter the side surface thereof;
A fifth substrate covering the fourth substrate,
Electrochemical detection sheet with blood filtration function.

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