JP3174082B2 - Blood component separation method and connected bag - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for separating blood components and a connected bag.

[0002]

2. Description of the Related Art At the time of blood transfusion, blood obtained from a donor is separated into various components by centrifugation or the like, for reasons such as effective use of blood and reduction of the burden on the translator. There is a system that only transfuses blood. With the introduction of this component blood transfusion, the blood is more effectively used as compared with the conventional whole blood transfusion.

[0003] In such a component transfusion, a connected blood bag (multiple bag) composed of a blood collection bag and one or more other bags is used.

[0004] As an example of this multiple bag,
A blood collection bag, a platelet storage bag (hereinafter referred to as a PC bag), a plasma storage bag (hereinafter referred to as a plasma bag), and an auxiliary bag for accommodating a buffy coat are connected by a tube, and blood is collected in the blood collection bag. The blood thus obtained is centrifuged to separate it into three components of red blood cells, platelet-rich platelets, and platelet-poor plasma, and these are stored and stored in a blood collection bag, a PC bag, and a plasma bag, respectively.

When storing red blood cells, useful components other than red blood cells are separated from the blood collection bag into child bags, and then a red blood cell storage solution is put into the blood collection bag in which the red blood cells are stored. The erythrocyte preservation solution is contained in a PC bag and is sent to the blood collection bag after the plasma and buffy coat have been separated. Thereafter, the concentrated platelets are transferred to the empty PC bag from the auxiliary bag that has been centrifuged again.

Here, the transfer of the red blood cell preservation solution is performed by positioning the PC bag at a higher position than the blood collection bag and providing a drop between the two bags. In the liquid transfer by such a method, a bag (blood collection bag) on the supply side is used.
Unless the head of the supply side (PC bag) is made large, a smooth and quick liquid transfer cannot be performed. Furthermore, since the bag is formed by stacking sheet materials, the empty PC bag becomes flat, but liquid remains between the contacted sheet materials due to the action of surface tension or the like. Also, the liquid remains in the tube.

On the other hand, in order to increase the drop, it is necessary to lengthen the tube connecting the bags, which is troublesome to handle.

[0008] The residual liquid has problems such as an improper mixing ratio of the red blood cell preservation liquid to the red blood cells, and when concentrated platelets are transferred to a PC bag, the red blood cell preservation liquid remaining in the tube or bag remains. There are drawbacks such as the liquid coming into contact with the platelets and adversely affecting the stored platelets.

[0009] Further, even in the case of a bag connected body used for other purposes, there is a problem that the liquid remaining in the tube is mixed when transferring another liquid and the liquid composition is changed. Have.

[0010]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a blood component separation method and a connected bag with a small amount of liquid remaining in a supply-side bag or a tube during liquid transfer.

[0011]

This and other objects are achieved by the present invention which is defined below as (1) to (6).

(1) A plurality of bags each having a storage portion formed by stacking flexible sheet materials, and a tube connected to the storage portion of each of the bags to connect the bags and feed liquid between the bags. A blood component separation method for separating and transferring blood components by using a bag connection body in which at least one of the bags is filled with a liquid and a sterile and dust-free gas is sealed. A step of transferring the liquid in the bag to another bag via the tube with the connection portion of the tube facing downward with the bag containing the liquid and the gas. Component separation method. (2) The plurality of bags include a blood collection bag, a plasma storage bag, and a bag containing the liquid and the gas, wherein the liquid is a red blood cell storage solution, and the other bag is a blood collection bag. The blood component separation method according to the above (1), which is a bag.

(3) a step of separating blood in the blood collection bag into red blood cells and plasma containing other components; and a step of discharging the plasma from the blood collection bag and leaving the red blood cells in the blood collection bag. After performing, the step of discharging the erythrocyte preservation solution in the bag containing the erythrocyte preservation solution and the gas with the connecting portion of the tube facing downward and transferring the erythrocyte preservation solution to the blood collection bag is performed (2). ). (4) The blood component separation method according to any one of (1) to (3), wherein after the liquid is discharged from the bag containing the liquid and the gas, blood components other than red blood cells are introduced into the bag. .

(5) A plurality of bags each having a storage portion formed by stacking flexible sheet materials, and a tube connected to the storage portion of each of the bags to connect the bags and feed liquid between the bags. The bag filled with the liquid was positioned higher than the other bags with the connecting portion of the tube facing downward, and the bag was filled with the liquid in the bag connected body for transferring the liquid to the other bag. A bag assembly comprising a bag provided with a ventilation portion for introducing gas into the bag, and the ventilation portion provided with a filter having air permeability and sealing means which can be opened when necessary. (6) The connected bag according to the above (5), wherein the filter has a dust removing and bacteria removing action.

[0015]

Since the bag filled with the liquid has a storage portion made of a flexible sheet material, deformation such as expansion and contraction easily occurs according to the amount of liquid in the bag.

The transfer of the liquid between the bags via the tube is performed by discharging the liquid in the bag by positioning the bag filled with the liquid at a high position or pressing the bag from outside. It is.

Here, if gas is sealed in the bag, a space is formed in the storage section. Therefore, by lowering the tube connection section, the liquid in the storage section can be easily transferred to the connection section of the tube. If it flows in, and if left untreated, a certain amount of swelling usually remains inside the bag, and the liquid corresponding to that amount remains, whereas the gas is sealed, so that the inside of the storage part Almost all of the liquid is discharged.
Since the gas is sterile and dust-free, it does not adversely affect the liquid in the container.

In filling the gas, first, the gas in the container is extracted, and the dust-free and sterile gas is quantitatively charged.

On the other hand, if a ventilation portion communicating with the inside of the storage portion is provided, a gas flows into the storage portion when the liquid is discharged, and the same operation is obtained. When discharging the liquid, the sealing means is first opened. And when the gas passes through the filter,
Bacteria and dust are removed and flow into the bag.

According to such a connected bag, the liquid can be transferred smoothly and quickly without providing a sufficient head.

[0021]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a blood component separation method and a connected bag of the present invention will be described in detail based on preferred embodiments shown in the accompanying drawings.

FIG. 1 shows an embodiment of a connected bag for performing the blood component separation method of the present invention. This connected bag is configured by connecting a blood collection bag, a PC bag, a plasma bag, and an auxiliary bag with a tube.

In FIG. 1, the blood collection bag 1 located on the right side
A flexible sheet material made of soft polyvinyl chloride (PVC) is stacked, and is fused (for example, heat-fused, ultrasonic-fused, or the like) at the peripheral seal portion 2 to form a bag. .

In the inner portion surrounded by the seal portion 2,
A blood component storage unit 3 is formed to store collected blood or concentrated red blood cells obtained by centrifugation from the blood.

At the upper part of the blood collection bag 1, there are formed two outlets 4, 4 provided with a peel tab, and between them, there is formed an outlet 5 for connecting to another bag described later. I have.

One end of a tube 14 is connected to the outlet 5 via a connecting member 15.

The other end of the tube 14 is connected to a first branch connector 20 which branches into two branches. A tube 21 is further connected to the branch connector 20, and a second branch connector 22 is connected to the other end of the tube 21.

The connecting member 15 is preferably a member whose flow passage is closed before the breakage, but which opens the flow passage when the breakage occurs. For example, a sealing member having a structure as described later with reference to FIG. A member can be used.

Further, one end of a flexible tube 6 is connected to the upper part of the blood collection bag 1 so as to communicate with the blood component storage part 3, and the other end of the tube 6 is connected to the blood collection via a hub 7. Needle 8 is mounted. Further, a cap 9 for enclosing the blood collection needle 8 is attached to the hub 7.

The blood component storage section 3 of the blood collection bag 1 contains an anticoagulant such as an ACD liquid, a CPD liquid, or a CPDA-1 liquid as required.

In FIG. 1, the plasma bag 10 located at the center
Stacks sheets made of soft polyvinyl chloride,
The peripheral seal portion 11 is formed into a bag by fusion (for example, heat fusion, ultrasonic fusion, or the like).

A plasma storage section 12 for storing plasma (particularly platelet poor plasma) separated from the blood in the blood collection bag 1 is formed in an inner portion surrounded by the seal section 11.

In the upper part of the plasma bag 10, two outlets 13 with a peel tab are formed.

Further, one end of a flexible tube 23 communicating with the plasma storage unit 12 is connected to the side of the outlet 13 at the upper part of the bag, and the other end of the tube 23 is connected to the branch connector 22. It is connected to one branch end.
Thereby, the blood component storage part 3 of the blood collection bag 1 and the plasma storage part 12 of the plasma bag 10
23 and the branch connectors 20 and 22.

The PC bag 30 located on the left side in FIG.
Are laminated with a sheet material made of soft polyvinyl chloride or the like, and fused (for example,
(Heat fusion, ultrasonic fusion, etc.).

A platelet storage portion 32 for storing platelets separated from blood is formed in an inner portion surrounded by the seal portion 31.

In the upper part of the PC bag 30, two outlets 33, 33 having a peel tab are formed. Further, a discharge port 34 for connecting to another bag is formed on the side of the discharge port 33 on the upper part of the bag.

One end of the flexible tube 24 is connected to the outlet 34 via a connecting member 25.

The other end of the tube 24 is connected to the other branch end of the branch connector 21. In addition,
As the connecting member 25, the same member as the sealing member used for the above-described connecting member 15 can be used. Thus, the platelet storage section 32 of the PC bag 30 and the blood component storage section 3 and the plasma storage section 12 of the other bags 1 and 10 are connected to the tubes 14, 21, 24 and the branch connectors 20, 2, respectively.
2 communicates.

The auxiliary bag 40 located on the left side of the blood collection bag 1 in FIG. 1 is a bag for mainly storing a buffy coat. The auxiliary bag 40 is formed by laminating a resin sheet material and fusing (for example, at a sealing portion 41 at the periphery thereof).
(Heat fusion, ultrasonic fusion, etc.).

A storage portion 42 for storing a buffy coat separated from the blood in the blood collection bag 1 is formed in an inner portion surrounded by the seal portion 41.

One end of a flexible tube 26 communicating with the storage section 42 is connected to the upper part of the auxiliary bag 40.
The other end of the tube 26 is connected to the first branch connector 2.
0 is connected to the branch end. Thereby, the storage part 42 of the auxiliary bag 40 and the blood component storage part 3, the plasma storage part 12, and the platelet storage part 32 of the other bags 1, 10, 30 are connected to the tubes 14, 21, 24, 26 and the branch connector. It communicates via 20 and 22.

The branch connectors 20 and 22 are made of a molded article made of a resin such as polyvinyl chloride or polycarbonate.

Next, constituent materials, sizes, and the like of each bag and tube in the illustrated connected bag will be described.

The blood collection bag 1 is made of soft polyvinyl chloride. The use of soft polyvinyl chloride provides flexibility to withstand centrifugation and heat resistance to sterilization.

The plasticizer that can be used in the blood collection bag 1 is not particularly limited, but di (ethylhexyl) phthalate (DEHP) is preferably used as the plasticizer.

By using this DEHP, when storing red blood cells, DEHP elutes into the blood collection bag, and the red blood cell membrane protective action suppresses hemolysis, thereby enabling long-term storage of red blood cells.

The content of the plasticizer DEHP is preferably 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride.

The reason is that if the amount is less than 30 parts by weight, the flexibility of the bag becomes poor.
This is because the amount of the plasticizer eluted into the bag increases, and the strength also decreases.

Further, a stabilizer or the like can be added to the sheet material of the blood collection bag 1. Examples of the stabilizer include an epoxy compound and a Ca—Zn-based stabilizer.

The thickness of the sheet material in the blood component storage section 3 of the blood collection bag 1 is preferably 0.30 to 0.40 mm,
It is more preferably 0.35 to 0.40 mm.

If the thickness of the sheet material is less than 0.30 mm, sufficient strength cannot be obtained, and if it exceeds 0.40 mm, the flexibility is reduced, which affects the operability of blood collection and the drainage ability at the time of blood transfusion. is there.

The capacity of the blood collection bag 1 is not particularly limited, but is preferably about 50 to 600 ml, more preferably 2 to 600 ml.
It is about 00 to 500 ml.

In general, a blood collection bag having a capacity of 200 ml or 400 ml is often used due to restrictions on the amount of blood collected.

The constituent material of the sheet material of the plasma bag 10 is not particularly limited, and may be the same as that of the blood collection bag 1 or the PC bag 30 described later.
It is preferable to use a polyolefin material such as plasticized PVC or ethylene-vinyl acetate copolymer (EVA).

The thickness of the sheet material in the plasma storage section 12 of the plasma bag 10 is preferably 0.20 to 0.4.
Approximately 5 mm. The gas permeability of the plasma bag 10 is not particularly limited, and may be lower than the gas permeability of the PC bag 30 described later.

The size of the plasma bag 10 is not particularly limited, but preferably has a capacity of about 100 to 400 ml.

The PC bag 30 is made of soft polyvinyl chloride. By using soft polyvinyl chloride, flexibility and heat resistance against sterilization that can withstand the centrifugation operation and the storage under shaking can be obtained, and wrinkles during centrifugation that affect the dispersibility of platelets are small. Furthermore, since it has flexibility, when shaking and storing platelets, uniform shaking becomes possible.

The thickness of the sheet material in the plasma storage section 12 of the PC bag 30 is 40 to 70%, preferably 40 to 60%, of the thickness of the sheet material in the blood component storage section 3 of the blood collection bag 1. More preferably, it is set to 40 to 50%.

In the bag connected body of the present embodiment, the PC bag 30 cannot be subjected to the centrifugal separation, so that the blood collection bag 1
The PC bag 30 does not need to be as strong (durable) as possible, and thus the PC bag 30 can be made thinner than the blood collection bag 1. However,
If the sheet thickness of the PC bag 30 is less than 40% of the sheet thickness of the blood collection bag 1, the strength of the PC bag also becomes insufficient and the PC bag 30 is easily broken. On the other hand, if it exceeds 70%, in order to obtain a desired gas permeability, it is necessary to increase the inner surface area of a portion which can be involved in gas permeability of the platelet storage section described later, and it is necessary to sufficiently reduce the size of the PC bag. I can't do it.

The plasticizer usable in the PC bag 30 is not particularly limited, but di (ethylhexyl) phthalate (DEHP) or di- (n-decyl) phthalate (DnDP) is preferably used as the plasticizer.

When DEHP is used as the plasticizer of the PC bag 30 as in the blood collection bag 1, the PC bag 3
By setting the thickness of the sheet material of No. 0 to 40 to 56% of the thickness of the sheet material of the blood collection bag 1, the capacity of the PC bag 30 can be reduced to about 20 to 75% of the capacity of the blood collection bag 1.

As a plasticizer for the PC bag 30, Dn
When DP is used, the gas permeability of the bag can be further improved.

The content of such a plasticizer is preferably 30 to 70 parts by weight based on 100 parts by weight of polyvinyl chloride.

The reason is that if the amount is less than 30 parts by weight, the flexibility of the bag becomes poor, the liquid discharging ability is reduced, and the liquid cannot be discharged quickly and reliably. The amount of plasticizer that elutes into
Further, the strength also decreases.

In the sheet material of the PC bag 30,
The same stabilizers and the like as described above can be added.

In the present invention, the constituent material of the PC bag 30 is plasticized P by the above-mentioned DEHP, DnDP or the like.
Not limited to VC, polyvinyl chloride containing no plasticizer (for example, non-plastic PVC: NP-resin, manufactured by Toagosei Co., Ltd.
Non-plastic PVC (Esmedica) manufactured by Sekisui Corporation may be used.

The platelet storage section 32 of the PC bag 30
CO ₂ gas permeation amount of preferably 3.1 ml (C
O ₂ ) / day · atm · ml (PC) (30 ° C.) or more, more preferably 3.6 ml (CO ₂ ) / day · atm · ml (PC) (30 ° C.) or more, and even more preferably 4.0 ml (PC). CO ₂ ) / day • atm • ml (PC) (30 ° C) or more is preferable.

The CO ₂ gas permeation amount is 3.1 ml (CO ₂ ) / day · at
If it is less than m · ml (PC) (30 ° C.), it will be difficult to store platelets for a long time.

The platelet storage section 32 of the PC bag 30
O ₂ gas permeation amount of preferably 0.6 ml (O ₂₎ / d
ay · atm · ml (PC) (30 ° C) or more, more preferably 0.7
It is preferably at least ml (O ₂ ) / day · atm · ml (PC) (30 ° C.), more preferably at least 0.8 ml (O ₂ ) / day · atm · ml (PC) (30 ° C.).

The O ₂ gas permeation amount is 0.6 ml (O ₂ ) / day · atm · m
If the temperature is less than l (PC) (30 ° C.), it will be difficult to store platelets for a long time.

With the configuration described above, a PC bag capable of storing at least one unit of platelets, particularly platelets having a platelet count of more than 4.5 × 10 ¹⁰ for a valid period of 72 hours or more is provided. .

The constituent material of the sheet material of the auxiliary bag 40 is not particularly limited, and may be the same as that of the blood collection bag 1 or the PC bag 30.

The thickness of the sheet material in the storage section 42 of the auxiliary bag 40 is preferably 0.30 to 0.40 mm.
Degree. The gas permeability of the auxiliary bag 40 is not particularly limited, and may be lower than the gas permeability of the PC bag 30. Although the size of the auxiliary bag 40 is not particularly limited,
For example, when the blood collection volume is 400 ml, a volume of about 80 to 150 ml is preferable.

The constituent material of the tubes 6, 14, 21, 23, 24 and 26 is preferably polyvinyl chloride. If the tubes 6, 14, 21, 23, 24, and 26 are made of polyvinyl chloride, sufficient flexibility and flexibility can be obtained, so that they are easy to handle, and are suitable for closing with a clamp or the like. This is because the bonding property with the connectors 20 and 22 is improved.

The tubes 6, 14, 21, 23, 2
The types and contents of the plasticizers 4 and 26 are not particularly limited. The inner diameter of the tubes 6, 14, 23, 24 and 26 is preferably about 2.0 to 4.5 mm.

In the connected bag constructed as described above, the erythrocyte preservation liquid 35 is filled in the platelet storage section 32 of the PC bag 30 before use, and
2 is filled with a sterile and dust-free gas. This erythrocyte preservation solution includes, for example, S. A. G. FIG. M. Liquid (0.877% sodium chloride, 0.0169% adenine, 0.818% grape, etc., an aqueous solution containing 0.525% D-mannitol) and the like. Further, examples of the gas to be sealed include an inert gas such as argon, neon, and helium, nitrogen, and air. By using such a gas and being dust-free and sterile, the gas has no adverse effect on the liquid in the bag.

Further, by filling such a gas,
When the erythrocyte preservation solution 35 is transferred to the blood collection bag 1, the entire amount of the erythrocyte preservation solution 35 can be discharged without leaving the erythrocyte preservation solution 35 in the platelet storage unit 32, and the tubes 24, 21, and 14 can be discharged. The amount of residue inside is also reduced. The amount of gas to be charged depends on the bag 30 regardless of the bag capacity.
Of the blood suspension bag 1 and the bottom suspension of the blood collection bag 1
If it is about 0 cm, it is about 2 to 30 m1, more preferably 3
It is good to be about 20 m1. If the value is less than the above value, the effect of gas filling decreases, and if the value is larger than the value,
This is because a sufficient storage capacity for the red blood cell preservation solution cannot be secured.

The method of filling the aseptic and dust-free gas will be described. The gas is sealed by a sealing device as shown in FIG. The illustrated device is configured by a gas suction system 51, a sealing system 52, and a chemical solution sending system 53,
The respective systems are switched by the switching valves 54 and 55, respectively.

Each bag was sterilized by, for example, autoclaving,
After sterilization by OG sterilization or the like, or before sterilization, as described above, the P
The C bag 30 is connected to the present apparatus, and first, the gas in the PC bag 30 is extracted by the pressure reducing device 511 by the suction system 51. The suction system 51 is provided with a pressure sensor 512, and the pressure reducing device 511 is controlled based on the sensor value. When the pressure reaches a predetermined pressure, the switching valve 54 switches to the sealing system 52. In addition, the suction system 51 is provided with a buffer valve 513 so that the pressure in the suction system 51 does not drop below a certain pressure, thereby preventing damage to the entire system. Reference numeral 514 in the figure is a pressure gauge.

Next, gas is sealed in the PC bag 30 from the sealing system 52. The gas is supplied to a gas compressor 52
1 and the bacteria are removed and the dust is removed by the gas filter 522.
It is sent into the bag 30. The amount of gas to be sealed is set in advance, and the amount of gas to be sealed is defined by sealing at a predetermined pressure for a predetermined time. Specifically, when the content of the PC bag 30 is about 150 to 300 ml, 0.5 to 5 g / cm ²
The pressure may be about 0.5 to 2 seconds. The pressure in the system is determined based on the detection value of the pressure sensor 523.

After the gas is filled, the switching valve 55 located downstream of the switching valve 54 is switched, and the red blood cell storage solution is sent from the liquid sending system 53. The erythrocyte preservation solution is supplied by a predetermined amount from the liquid supply pump 531. At this time, bacteria removal and dust removal are performed by the filter 532. As described above, PC
The gas and the erythrocyte preservation solution are sealed in the bag 30. The order of injection of the gas and the erythrocyte preservation solution may be reversed.

Next, a blood component separation method of the present invention using the illustrated connected bag will be described.

1) The blood collection bag 1 is placed in a low place or placed in an automatic blood collection device, and the blood collection needle 8 is punctured into the blood vessel of the blood collector with the tube 14 closed (before the connection member 15 is broken). Blood collection. Thereby, the collected blood is introduced into the blood storage section 3 of the blood collection bag 1 via the tube 6.

2) Close the tube 6 with a clamp or the like,
The blood collection bag 1 is centrifuged to separate red blood cells and plasma containing other components.

3) The tubes 24 and 26 are closed with a clamp or the like, and the tube 14 is released (the sealing member of the connecting member 15 is broken), and the blood collection bag 1 is pressed to separate the separated supernatant plasma. Is introduced into the plasma storage section 12 of the plasma bag 10 via the tubes 14, 21 and 23.

4) When the buffy coat located at the interface between the plasma and the red blood cells reaches the upper end of the blood storage section 3, the pressing of the blood collection bag 1 is stopped, and the tube 23 is closed with a clamp or the like.

5) The blockage of the tube 26 is released, and the blood collection bag 1 is further pressed to introduce the buffy coat in the blood collection bag 1 into the storage section 42 of the auxiliary bag 40 via the tube 26.

6) After the buffy coat in the blood collection bag 1 and the introduction of a small amount of red blood cells are completed, the tube 14 is closed with a clamp or the like, and the blockage of the tube 23 is released, and a small amount of plasma is removed from the plasma bag 10 to the auxiliary bag. Introduce to 40.

7) When the introduction is completed, the tube 2
3 and 26 are closed with a clamp or the like, and the blockage of the tube 24 is released (breakage of the sealing member of the connecting member 25).

8) The PC bag 30 is placed above the blood collection bag 1 with the discharge port 34 downward, and the red blood cell storage solution 35 is introduced into the blood collection bag 1 by the head of both bags. At this time, the gas 36 in the PC bag 30
On the other side (upper side) of 4, the red blood cell storage solution 35 flows out from below. The gas 35 forms a space in the PC bag 30 and does not remain between the sheet materials closely contacted by the surface tension, and substantially the entire amount is discharged from the PC bag 30. Further, the gas in the PC bag 30 is supplied to the tube 2
4, 21, and 14, the amount of the red blood cell storage solution 35 remaining in the tube is also reduced. Further, since the liquid can be discharged without providing a sufficient head, the length of the tube may be short.

9) The blood collection bag 1 and the plasma bag 10 are cut off as shown in FIG. 3 by sealing and cutting the tubes 14 and 23 with a tube sealer.

10) Auxiliary bag 40 and PC bag 30
Is centrifuged again to separate the blood in the auxiliary bag 40 into concentrated platelets, red blood cells, and white blood cells.

11) The concentrated platelets separated upward in the auxiliary bag 40 are introduced into the PC bag 30 to obtain a concentrated platelet preparation.

12) The auxiliary bag 40 is a tube 26
Seal and cut with a tube sealer and discard.

As described above, when transferring the concentrated platelets, the amount of the erythrocyte preservation solution remaining in the PC bag 30 or the tube is very small, so that the adverse effects of the erythrocyte preservation solution on the concentrated platelets are extremely small.

As a means for transferring the red blood cell storage solution from the PC bag 30 to the blood collection bag 1, the PC bag 30 may be pressed from the outside with the outlet 34 facing downward.
In this case, the inner diameter of the tubes 24, 21, 14 is
About 2.0 to 4.0 mm, more preferably 2.5 to 3.5 mm
It is good to be about mm.

Next, an embodiment of the bag connecting body of the present invention shown in FIG. 4 will be described. In this embodiment, the PC
A ventilation part 60 is provided in the bag 30. Ventilation section 60
Is provided at the end opposite to the discharge port 34, and includes a tube 62, a sealing means 61, and a filter 64. The tube 62 communicates with the inside of the platelet storage section 32, and a sealing means 61 is connected to a distal end thereof as shown in FIG. The sealing means 61 includes a cylindrical body 611 made of a flexible material similar to the tube,
And a sealing member 612 tightly fixed therein. The sealing member 612 is a cylindrical body whose one end is closed, and has a small-diameter broken portion 613 formed on the outer periphery. The closed portion (solid column-shaped portion) 614 is refracted and broken by the broken portion 613 to allow communication. It is configured so that The material of the sealing member 612 may be, for example, polyvinyl chloride, polycarbonate, thermoplastic polyester elastomer, or the like.

A filter 64 is connected to the other end of the sealing means 61 via a tube 62. Filter 6
4 is provided with a tubular filter holding member 63 and connected to a tube 62.

[0100] If necessary, a flat protruding portion 615 having a long side corresponding to the outer diameter of the solid columnar portion 614 (in FIG. The short side when viewed from the direction perpendicular to the side is shown.)
Is formed, and after the solid columnar portion 614 is cut, the solid columnar portion 6 is formed.
It is desirable that the fourteen sides do not block the tubular body 62.

On the other hand, a filter 64 that allows gas to pass through but does not pass blood is attached to the end of the tubular body 62.

The filter 64 has a dust-removing and bacteria-removing action. For example, the filter 64 may be made of a sintered body formed by powdering polyethylene, polypropylene, nylon, or the like into a mold, and then heating and compressing the molded body. .

Instead of the above sintered body, a membrane filter in which a polyvinyl chloride is coated on a polyester resin aggregate may be used.

As a material of the membrane filter, a material obtained by laminating polytetrafluoroethylene on a polyethylene nonwoven fabric, a material obtained by coating an acetate film with a silicone resin, and the like can be used.

The airflow resistance of the filter 64 is preferably about 1 to 5 m1 / sec when the pressure difference is 0.2 kg / cm ² , and more preferably about 3 to 5 m1. .

Here, the order of connection between the sealing means 61 and the filter 64 may be reversed. Sealing means 61
When discharging the red blood cell preservation solution from the PC bag 30,
By breaking the solid columnar portion 614 of the sealing member 612, the ventilation portion 6
Release 0.

With the above configuration, P
When the erythrocyte preservation solution is transferred from the C bag 30 to the blood collection bag 1, gas is introduced from the ventilation section 60 as needed with the discharge of the erythrocyte preservation solution, so that the discharge of the erythrocyte preservation solution is substantially the same as in the first embodiment. Completely done.

It is to be noted that a configuration may be employed in which pressurized gas is forcibly fed into the bag from the ventilation section 60 by using, for example, a compressor or an air pump. In this case, the gas to be fed is previously made dust-free and sterile. For example, the filter may be a simple air-permeable filter such as polypropylene, polyvinylidene dichloride, and polytetrachloroethylene.

The present invention is not limited to the connected bag of the above embodiment, but may be any connected bag that introduces liquid between the bags, and is not limited to the above-mentioned applications.

[0110]

As described above, according to the present invention,
Discharge of the liquid from the bag can be performed quickly and almost completely, and the residual liquid in the tube is reduced, eliminating the disadvantages that the liquid in the bag is adversely affected by the mixing of the residual liquid and the liquid composition is modulated. Is done. In addition, since it is not necessary to provide a high drop to transfer the liquid, the length of the tube can be shortened, and a compact connected bag is provided.

[Brief description of the drawings]

FIG. 1 is an overall side view showing a connected bag used in the present invention.

FIG. 2 is a schematic diagram illustrating a configuration of a sealing device for sealing a gas into a bag.

FIG. 3 is an overall side view showing a state in which a plasma bag and a blood collection bag are removed from a connected bag.

FIG. 4 is an overall side view showing the connected bag of the embodiment of the present invention.

FIG. 5 is a sectional view of a ventilation section provided in the bag.

[Explanation of symbols]

 REFERENCE SIGNS LIST 1 blood collection bag 2 seal portion 3 blood component storage portion 4, 5 outlet 6 tube 7 hub 8 blood collection needle 9 cap 10 plasma bag 11 seal portion 12 platelet storage portion 13 outlet 14 tube 15 connecting member 20, 22 branch connector 21 tube 23, 24 Tube 26 Tube 30 PC bag 31 Seal part 32 Platelet storage part 33 Outlet 34 Outlet 35 Red blood cell storage liquid 36 Gas 40 Auxiliary bag 41 Seal part 42 Storage part 50 Enclosure device 51 Suction system 511 Pressure reducing device 512 Pressure sensor 513 Buffer valve 514 Pressure gauge 52 Sealing system 521 Gas compressor 522 Gas filter 523 Pressure sensor 53 Liquid sending system 531 Liquid sending pump 532 Filter 54, 55 Switching valve 60 Ventilation part 61 Sealing means 611 Cylindrical body 612 Sealing member 13 breaks 614 closed end 62 tubes 63 filter holding member 64 filter

──────────────────────────────────────────────────続 き Continued on the front page (58) Field surveyed (Int. Cl. ⁷ , DB name) A61J 1/00-1/05 A61M 1/00-1/02

Claims (6)

(57) [Claims] 1. A plurality of bags each having a storage portion formed by laminating flexible sheet materials, and a tube connected to the storage portion of each of the bags to connect the bags and perform liquid transfer between the bags. A blood component separation method for separating and transferring blood components using a bag connector in which at least one of the bags is filled with a liquid, and a sterile and dust-free gas is sealed, A blood component comprising: placing a bag containing the liquid and the gas with the connection portion of the tube facing downward, and transferring the liquid in the bag to another bag via the tube. Separation method. 2. The plurality of bags include a blood collection bag, a plasma storage bag, and a bag containing the liquid and the gas, wherein the liquid is a red blood cell storage solution, and the other bag is The blood component separation method according to claim 1, which is a blood collection bag. 3. A step of separating blood in the blood collection bag into red blood cells and plasma containing other components, and a step of discharging the plasma from the blood collection bag to leave the red blood cells in the blood collection bag. The step of discharging the erythrocyte preservation solution in the bag containing the erythrocyte preservation solution and the gas with the connection portion of the tube facing downward, and transferring the erythrocyte preservation solution to the blood collection bag. The method for separating blood components according to the above. 4. The blood component separation method according to claim 1, wherein after the liquid is discharged from the bag containing the liquid and the gas, blood components other than red blood cells are introduced into the bag. 5. A plurality of bags each having a storage portion formed by stacking flexible sheet materials, and a tube connected to the storage portion of each bag to connect each bag and feed liquid between the bags, The bag-filled body, wherein the bag filled with the liquid is positioned higher than the other bag with the connection portion of the tube facing downward, and the liquid is transferred to the other bag. , A vented portion for introducing a gas into the bag, and the vented portion is provided with a filter having air permeability and sealing means that can be opened when necessary. 6. The bag assembly according to claim 5, wherein the filter has a dust removing and bacteria removing action.