JP3153418B2 - Sample holder for electron spin resonance - Google Patents
Sample holder for electron spin resonanceInfo
- Publication number
- JP3153418B2 JP3153418B2 JP17231694A JP17231694A JP3153418B2 JP 3153418 B2 JP3153418 B2 JP 3153418B2 JP 17231694 A JP17231694 A JP 17231694A JP 17231694 A JP17231694 A JP 17231694A JP 3153418 B2 JP3153418 B2 JP 3153418B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- concave portion
- electron spin
- spin resonance
- sample holder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、電子スピン共鳴（ＥＳ
Ｒ）装置用試料ホルダに関し、特に生体組織や細胞を生
きたまま測定するのに用いて好適な試料ホルダに関す
る。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to electron spin resonance (ES).
R) The present invention relates to a sample holder for an apparatus, and more particularly to a sample holder suitable for use in measuring living tissue and cells alive.
【０００２】[0002]
【従来の技術】生体組織や細胞を生きたままＥＳＲ測定
する際、従来は、例えば図１に示すような試料ホルダ
（ティッシュセル）が用いられている。図１において、
ホルダの挿入棒Ｏの先端にセル部１が設けられており、
このセル部に凹部２が掘られている。培養液（生理食塩
水）に浸された試料の一部を取って凹部２の底に置き、
凹部２の上にカバー３を被せ、カバー３をクリップ４で
止めた後、セル部１を空胴共振器内に挿入してＥＳＲ測
定が行われる。2. Description of the Related Art Conventionally, when a living tissue or a cell is subjected to ESR measurement while being alive, a sample holder (tissue cell) as shown in FIG. 1 is conventionally used. In FIG.
The cell part 1 is provided at the tip of the insertion rod O of the holder,
A concave portion 2 is dug in this cell portion. Take a part of the sample immersed in the culture solution (physiological saline) and place it on the bottom of the concave portion 2,
After covering the concave portion 2 with the cover 3 and fixing the cover 3 with the clip 4, the ESR measurement is performed by inserting the cell portion 1 into the cavity resonator.
【０００３】[0003]
【発明が解決しようとする課題】この様な従来の構造で
は、生体組織や細胞と反応する薬物などとの反応を調べ
るのに必要な反応液の迅速な交換ができない。また、測
定が済んで試料を除去する際、ホルダ全体を洗浄する必
要があり時間がかかる。さらに、凹部の形状によっては
洗浄自体が難しいため、試料が凹部の隅に残留しやす
く、正確な測定が期待できない場合もある。With such a conventional structure, it is not possible to quickly exchange a reaction solution necessary for examining a reaction between a living tissue or a cell and a drug which reacts. Further, when the sample is removed after the measurement, it is necessary to clean the entire holder, which takes time. Furthermore, since the cleaning itself is difficult depending on the shape of the concave portion, the sample tends to remain at the corner of the concave portion, and accurate measurement may not be expected in some cases.
【０００４】本発明は、上述した点に鑑みてなされたも
のであり、ホルダ全体を洗浄する必要がなく、試料セル
の開閉を行うことなく簡単に試料交換を行うことができ
る電子スピン共鳴装置用試料ホルダを提供することを目
的としている。[0004] The present invention has been made in view of the above points, and does not require cleaning of the entire holder, and is intended for an electron spin resonance apparatus capable of easily exchanging a sample without opening and closing the sample cell. It is intended to provide a sample holder.
【０００５】さらに、本発明は、セル内に培養液などを
連続的に供給することの出来る電子スピン共鳴装置用試
料ホルダを提供することを目的としている。Another object of the present invention is to provide a sample holder for an electron spin resonance apparatus capable of continuously supplying a culture solution or the like into a cell.
【０００６】[0006]
【課題を解決するための手段】この目的を達成するた
め、本発明の電子スピン共鳴装置用試料ホルダは、凹部
を有する保持部材と、試料をその表面に保持し該凹部内
に収容される板体と、該板体を内部に収容したまま凹部
に被せられるカバー体と、前記凹部に培養液や化学的薬
剤などを流すための導入流路及び排出流路とから構成さ
れることを特徴としている。In order to achieve this object, a sample holder for an electron spin resonance apparatus according to the present invention comprises a holding member having a concave portion, and a plate holding the sample on its surface and housed in the concave portion. Body, a cover body that is placed over the recess while the plate body is housed therein, and an introduction flow path and a discharge flow path for flowing a culture solution, a chemical agent, and the like into the depression. I have.
【０００７】本発明の電子スピン共鳴装置用試料ホルダ
は、さらに前記板体の試料を保持する面に細かな凹凸が
設けられていることを特徴としている。[0007] The sample holder for an electron spin resonance apparatus according to the present invention is characterized in that fine irregularities are provided on the surface of the plate body for holding the sample.
【０００８】[0008]
【作用】本発明においては、試料をその表面に保持し該
凹部内に収容される板体を設けたため、試料交換にあた
って板体を交換すれば良い。In the present invention, since the plate is held on the surface of the sample and accommodated in the recess, the plate may be replaced when the sample is replaced.
【０００９】[0009]
【実施例】以下、図面に基づいて本発明の一実施例を詳
説する。図２は本発明の一実施例を示しており、図１の
従来例と同一の構成要素には同一の番号が付されてい
る。図２において、サンプルシート５は厚さが数１０〜
数１００μｍ程度の薄いガラス板で、片面がすりガラス
状に加工されており、試料を取ってその面に試料を付着
させた時に試料がより定着しやすくなっている。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below in detail with reference to the drawings. FIG. 2 shows an embodiment of the present invention, and the same components as those in the conventional example of FIG. 1 are denoted by the same reference numerals. 2, the sample sheet 5 has a thickness of several tens to ten.
A thin glass plate having a thickness of about several hundred μm, one surface of which is processed into a frosted glass shape, makes it easier to fix the sample when the sample is taken and the sample is attached to the surface.
【００１０】そして、試料を片面に支持したサンプルシ
ート５は、ホルダ先端の凹部２に試料載置面を上にして
セットされ、その上にカバー３が被せられる。[0010] The sample sheet 5 supporting the sample on one side is set in the concave portion 2 at the tip of the holder with the sample mounting surface facing up, and the cover 3 is placed on the sample sheet 5.
【００１１】サンプルシートは基本的に使い捨てとさ
れ、測定前の準備作業で、複数の試料は別々の新しいサ
ンプルシートに乗せられる。試料交換は、この様にして
準備したサンプルシートを次々に取換えて凹部にセット
すれば良く、ホルダの洗浄など面倒な作業が不要で、短
時間に行うことができる。The sample sheet is basically disposable, and a plurality of samples are placed on separate new sample sheets in a preparation operation before measurement. The sample exchange can be performed in a short time by replacing the sample sheets prepared in this way one after another and setting the sample sheet in the concave portion.
【００１２】なお、サンプルシートは、上述した本来の
使用形態の他に、たとえば、細長くてカールするような
固体試料を押さえて伸ばす役目を与えることも出来る。[0012] In addition to the above-mentioned original usage, the sample sheet can also serve to press and stretch a solid sample that is elongated and curls, for example.
【００１３】図３は本発明の他の実施例を示す図であ
る。本実施例では、凹部２内に溶液を供給するための送
液通路６が挿入棒Ｏの中に設けられ、さらに、セル部１
の先端には凹部２内から溶液を排出するための排液管７
及び排液通路８が設けられている。そして、液漏れを防
ぐため、セル部１の凹部周囲の縁の部分には、シール剤
が塗られ、その上に押し付けるようにしてカバー３がセ
ットされる。FIG. 3 shows another embodiment of the present invention. In this embodiment, a liquid feed passage 6 for supplying a solution into the concave portion 2 is provided in the insertion rod O, and further, the cell portion 1
A drain pipe 7 for discharging the solution from the inside of the concave portion 2
And a drain passage 8. Then, in order to prevent liquid leakage, a sealing agent is applied to an edge portion around the concave portion of the cell portion 1, and the cover 3 is set so as to be pressed thereon.
【００１４】本実施例では、図２の実施例と同様にセッ
トされたサンプルシート５の表面の生体組織や細胞に、
送液通路６及び排液通路８を介して酸素を含む培養液を
供給し、排出することができるため、試料を長時間にわ
たって生きたままの状態で測定することができる。ま
た、培養液ばかりでなく、化学的薬剤などを供給しなが
らＥＳＲ測定を行うことも可能で、これにより、細胞の
発生する活性酸素の検出や特定物質の消去能の評価など
を行うことができる。In the present embodiment, living tissues and cells on the surface of the sample sheet 5 set in the same manner as in the embodiment of FIG.
Since the culture solution containing oxygen can be supplied and discharged through the liquid supply passage 6 and the drainage passage 8, the sample can be measured in a living state for a long time. It is also possible to perform ESR measurement while supplying not only a culture solution but also a chemical agent and the like, whereby it is possible to detect active oxygen generated by cells and to evaluate the ability to erase a specific substance. .
【００１５】シール性をより良くするためには、シール
材を介在させるカバー３とセル部との接触部分の面積を
大きくすることが好ましい。図４はカバーとセルのシー
ルについて説明するための断面図である。図４（ａ）は
図３の実施例の場合を示しており、単にセルの上にカバ
ーをのせるだけの構造である。図４（ｂ）は縁の部分を
折り曲げた形のカバーを用いた例を示し、図４（ｃ）は
セル部１にカバー３を受ける段差を設けた例を示してい
る。図４（ｂ）の例が最も接触面積が大きくシール性が
高い。In order to further improve the sealing property, it is preferable to increase the area of the contact portion between the cover 3 and the cell portion where the sealing material is interposed. FIG. 4 is a cross-sectional view for explaining the seal between the cover and the cell. FIG. 4A shows the case of the embodiment of FIG. 3, which has a structure in which a cover is simply placed on a cell. FIG. 4B shows an example in which a cover having a bent edge portion is used, and FIG. 4C shows an example in which a step for receiving the cover 3 is provided in the cell portion 1. The example of FIG. 4B has the largest contact area and the highest sealing performance.
【００１６】[0016]
【発明の効果】以上詳述したごとく、本発明では試料を
その表面に保持し該凹部内に収容される板体とともに、
該凹部内に酸素を含む培養液や化学的薬剤などを流すた
めの導入流路及び排出流路を設けたため、ホルダ全体を
洗浄する必要がなく、簡単に試料交換を行え、かつ、測
定中にセル内に酸素を含む培養液や化学的薬剤などを供
給することができる電子スピン共鳴装置用ホルダが提供
される。As described above in detail, according to the present invention, the sample is held together with the plate held on the surface thereof and accommodated in the recess.
Since the introduction flow path and the discharge flow path for flowing a culture solution or a chemical agent containing oxygen in the concave portion are provided, it is not necessary to clean the entire holder, the sample can be easily exchanged, and during the measurement, An electron spin resonance apparatus holder capable of supplying a culture solution containing oxygen, a chemical agent, or the like into a cell is provided.
【００１７】[0017]
【図１】 生体組織や細胞を生きたままＥＳＲ測定する
際に用いられる試料ホルダ（ティッシュセル）の従来例
を示す図である。FIG. 1 is a diagram showing a conventional example of a sample holder (tissue cell) used when performing ESR measurement while living tissues and cells are alive.
【図２】 本発明の一実施例を示す図である。FIG. 2 is a diagram showing one embodiment of the present invention.
【図３】 本発明の一実施例を示す図である。FIG. 3 is a diagram showing one embodiment of the present invention.
【図４】 カバーとセルのシールについて説明するため
の断面図である。FIG. 4 is a cross-sectional view for explaining a seal between a cover and a cell.
１ セル部 ２ 凹部 ３ カバー ４ クリップ ５ サンプルシート ６ 送液通路 ７ 排液管 ８ 排液通路 DESCRIPTION OF SYMBOLS 1 Cell part 2 Depression 3 Cover 4 Clip 5 Sample sheet 6 Liquid feed passage 7 Drain pipe 8 Drain passage
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 実開 昭63−118552（ＪＰ，Ｕ) 実開 昭57−75558（ＪＰ，Ｕ) (58)調査した分野(Int.Cl.7，ＤＢ名) G01N 1/28 G01N 24/10 510 ──────────────────────────────────────────────────続 き Continuation of the front page (56) References Japanese Utility Model Sho 63-118552 (JP, U) Japanese Utility Model Sho 57-75558 (JP, U) (58) Fields investigated (Int. Cl. 7 , DB name) G01N 1/28 G01N 24/10 510
Claims (2)
に保持し該凹部内に収容される板体と、該板体を内部に
収容したまま凹部に被せられるカバー体と、前記凹部に
培養液や化学的薬剤などを流すための導入流路及び排出
流路とから構成される電子スピン共鳴装置用試料ホル
ダ。1. A holding member having a concave portion, a plate body holding a sample on its surface and housed in the concave portion, a cover body covered with the plate body housed inside the concave portion, A sample holder for an electron spin resonance apparatus comprising an introduction channel and a discharge channel for flowing a culture solution, a chemical agent, and the like.
が設けられていることを特徴とする請求項１記載の電子
スピン共鳴装置用試料ホルダ。2. The sample holder for an electron spin resonance apparatus according to claim 1, wherein fine irregularities are provided on a surface of the plate body for holding the sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17231694A JP3153418B2 (en) | 1994-07-25 | 1994-07-25 | Sample holder for electron spin resonance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17231694A JP3153418B2 (en) | 1994-07-25 | 1994-07-25 | Sample holder for electron spin resonance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0835919A JPH0835919A (en) | 1996-02-06 |
| JP3153418B2 true JP3153418B2 (en) | 2001-04-09 |
Family
ID=15939661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17231694A Expired - Fee Related JP3153418B2 (en) | 1994-07-25 | 1994-07-25 | Sample holder for electron spin resonance |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3153418B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2551370A (en) * | 2016-06-16 | 2017-12-20 | Margaret Evans Pamela | Travel bag with attached pillow |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9620205D0 (en) * | 1996-09-27 | 1996-11-13 | Walsh Valentine | A mould |
| JP5260463B2 (en) | 2009-10-08 | 2013-08-14 | 株式会社日立製作所 | Sample holder for electric detection electron spin resonance apparatus |
-
1994
- 1994-07-25 JP JP17231694A patent/JP3153418B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2551370A (en) * | 2016-06-16 | 2017-12-20 | Margaret Evans Pamela | Travel bag with attached pillow |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0835919A (en) | 1996-02-06 |
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