JP3150453U - Nucleic acid amplification analysis auxiliary equipment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a nucleic acid amplification analysis auxiliary device for discriminating the presence or absence of a nucleic acid in an aqueous solution inexpensively, simply and safely. In the present invention, a belt-like heater 2 is attached and heated around a metal support 1 that is in close contact with a transparent container that supports an aqueous solution of nucleic acid, covered with a heat insulating material 3, and passed through a temperature sensor 4. Thus, there is provided an amplification analysis auxiliary device for determining the presence or absence of nucleic acid, characterized in that the temperature of the nucleic acid aqueous solution in the transparent container is maintained at an appropriate temperature by adjusting the temperature with the temperature controller 5. When a specific part of a living organism's DNA is amplified and its genotype is determined by the presence or absence of the amplification, a substance that binds to the amplified DNA and emits fluorescence is added, and then the reaction is carried out in a light-transmitting support. By carrying it out and placing it on the transilluminator 6 as it is, the genotype can be determined by observing the light emission state, and inexpensive, simple and safe nucleic acid amplification analysis is achieved. [Selection] Figure 1

Description

  The present invention relates to the presence or absence of nucleic acid, characterized in that the aqueous nucleic acid solution in the transparent container is heated via the support by heating the support closely contacting the transparent container supporting the aqueous nucleic acid solution. The present invention relates to a nucleic acid amplification analysis auxiliary device for discrimination.

  A person skilled in the art knows how to amplify a specific portion of DNA (deoxyribonucleic acid) of an organism using nucleic acid polymerase and determine the sequence (genotype) of the specific portion of DNA using the amplified DNA fragment as an indicator. It has been. Usually, in this method, it is indispensable to confirm the presence of the amplified fragment and the length of the fragment by electrophoresis using agarose or the like, which requires labor, time and cost.

Electrophoresis requires an expensive agarose gel and has a large economic burden, and it is dangerous because it uses a stain containing a chemical substance such as ethidium bromide that is carcinogenic. Furthermore, time, labor and space are required. An apparatus for discriminating the presence or absence of nucleic acids in a solution without an operation step that requires contact with the solution, such as electrophoresis, is desired.
An object of the present invention is to provide a nucleic acid amplification analysis auxiliary device for discriminating the presence or absence of a nucleic acid in an aqueous solution inexpensively, simply and safely.

The present invention is characterized in that a belt-like heater is mounted around a metal support that is in close contact with a transparent container that supports the nucleic acid aqueous solution, and the nucleic acid aqueous solution in the transparent container is heated via the metal support. The above problem is solved by providing an amplification analysis assisting device for determining the presence or absence of nucleic acid.
According to the above configuration, when a specific part of the DNA of an organism is amplified using a nucleic acid polymerase and the genotype is determined based on the presence or absence of the amplification, a substance that binds to the amplified DNA and emits fluorescence is added. In addition, the reaction is carried out in a light-transmitting support, and the support is placed on a transilluminator, and the state of light emission is photographed, so that the genotype can be determined, and it is inexpensive, simple and safe. Nucleic acid amplification analysis is achieved. Furthermore, by heating the support at about 60 ° C. to 75 ° C., fluorescence noise due to non-specific amplification products such as primer dimers can be reduced, and a clearer determination can be made.

  As described above, the apparatus of the present invention can clearly determine the presence or absence of nucleic acid amplification inexpensively, simply and safely without performing gel electrophoresis that is usually necessary after the PCR reaction.

1 is an overall view showing an embodiment of an auxiliary device for determining the presence or absence of a nucleic acid according to the present invention. It is a partially cutaway sectional view of the auxiliary device of the present invention.

  Aluminum or the like that is in close contact with a transparent container (such as a 96-well or 384-well polypropylene plate for PCR) supporting a nucleic acid aqueous solution and has a small hole for light passing through the bottom of the hole corresponding to each well. A ribbon-shaped heater is wound around the outer periphery of the metal support. A temperature sensor is sandwiched between the heater and the metal support, and the temperature of the metal support is maintained at 65 ° C to 75 ° C by a temperature controller. Insulate the outside of the heater to prevent burns. By placing a transparent container with an aqueous nucleic acid solution on a heat-insulated metal support and placing it on a transilluminator as it is, the state of light emission is photographed. Can be distinguished.

    An embodiment will be described below with reference to the accompanying drawings. Reference numeral 1 denotes a support having 96 (8 × 12) holes formed of an appropriate material having excellent thermal conductivity such as aluminum. 2 is a ribbon heater for heating 1 to an appropriate temperature, and 2a is an electric cord for energizing the ribbon heater. Reference numeral 3 denotes a heat insulating material for keeping the heated 1 warm and preventing burns on the fingers of the operator. 4 is a temperature sensor for measuring the temperature of 1, and 5 is a temperature controller for adjusting the temperature based on the measured temperature.

  6 is a transilluminator. That is, the temperature controller 5 is set between 65 ° C. and 75 ° C., the power is turned on, and after the metal support 1 is sufficiently warmed, the plate after PCR reaction (not shown) is inserted and placed on the transilluminator 6 for observation. By doing so, the presence or absence of nucleic acid amplification can be converted into the presence or absence of light emission and determined with the naked eye.

    Note that the number of holes in the support 1 may not be 96, and the material is not limited to metal, but may be another material such as resin. Alternatively, the support may be prepared by sealing a liquid substance in a sheet molded in the form of a support. As the ribbon heater 2, any shape heater can be equally used as long as it can heat the support 1 to an appropriate temperature. In addition, without using a heater, the support 1 can be warmed by placing it in a hot water bath or a heat retaining device, and a transparent container that supports the nucleic acid aqueous solution can be attached so that the temperature can be quickly observed before the temperature drops. It is.

DESCRIPTION OF SYMBOLS 1 Support body 2 Ribbon heater 3 Thermal insulation material 4 Temperature sensor 5 Temperature controller 6 Transilluminator

Claims (3)

  Nucleic acid amplification for determining the presence or absence of nucleic acid, comprising heating a support closely contacting a transparent container supporting an aqueous nucleic acid solution, and heating the aqueous nucleic acid solution in the transparent container via the support Analysis aid   The nucleic acid amplification analysis auxiliary device according to claim 1, wherein the support that is in close contact with the transparent container that supports the nucleic acid aqueous solution is made of metal.   The nucleic acid amplification analysis auxiliary device according to claim 1, wherein the heating method of the support closely contacting the transparent container supporting the nucleic acid aqueous solution is a belt-shaped heater.

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