JP3149253B2 - Method for inhibiting the growth of Salmonella - Google Patents
Method for inhibiting the growth of SalmonellaInfo
- Publication number
- JP3149253B2 JP3149253B2 JP05446892A JP5446892A JP3149253B2 JP 3149253 B2 JP3149253 B2 JP 3149253B2 JP 05446892 A JP05446892 A JP 05446892A JP 5446892 A JP5446892 A JP 5446892A JP 3149253 B2 JP3149253 B2 JP 3149253B2
- Authority
- JP
- Japan
- Prior art keywords
- salmonella
- growth
- inhibiting
- present
- galactooligosaccharides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Fodder In General (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、サルモネラ菌の増殖阻
害方法に関し、より詳しくは、本発明は、ガラクトオリ
ゴ糖を動物に摂取させて動物体内のサルモネラ菌の増殖
を阻害する方法に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for inhibiting the growth of Salmonella, and more particularly to a method for inhibiting the growth of Salmonella in an animal by ingesting galactooligosaccharides to the animal.
【０００２】[0002]
【従来の技術】サルモネラ菌は自然界に生息する、爬虫
類、両棲類、鳥類、家畜及び人間を含むあらゆる哺乳類
から分離されており、かなり広範囲に分布し、汚染源と
しての危険性を持っている。現在、我が国では、年間約
１００件近い、サルモネラ菌を原因とする食中毒が発生
している。この多くは、食肉及びその加工調理を原因と
するものである。食用動物に存在するサルモネラ菌の増
殖を阻害する公知の方法としては、飼料中に抗生物質等
の薬剤を添加して飼育する方法があるが、人体への影響
を考えるとこの方法は好ましくはない。BACKGROUND OF THE INVENTION Salmonella has been isolated from any mammal, including reptiles, amphibians, birds, livestock and humans, which inhabits the natural world, is quite widely distributed, and poses a risk as a source of contamination. At present, nearly 100 cases of food poisoning due to Salmonella occur annually in Japan. This is mostly due to meat and its processing and cooking. As a known method for inhibiting the growth of Salmonella present in edible animals, there is a method of breeding by adding a drug such as an antibiotic to a feed, but this method is not preferable considering the effect on the human body.
【０００３】最近、フラクトオリゴ糖を用いて、サルモ
ネラ菌の増殖を阻害する例（特許出願公表平３−５０１
９７１）がある。 しかし、フラクトオリゴ糖は、加
熱、酸に対して不安定であり、飼料として加工する際に
一部が分解し、オリゴ糖含有量が減少するという欠点が
ある。これに対して、ガラクトオリゴ糖は、熱や酸に対
して安定であり、飼料としての加工にも十分耐えられ
る。しかしながら、ガラクトオリゴ糖をサルモネラ菌の
増殖の阻害に利用することについては、今だ報告がな
い。又、ガラクトオリゴ糖は、食品成分として安全であ
ることが確認されており、食用動物の体内に残存しても
抗生物質のように人体への危険性は無い。Recently, an example of inhibiting the growth of Salmonella using fructooligosaccharides (Patent Application Publication No. 3-501)
971). However, fructooligosaccharides are unstable to heat and acid, and have a drawback that a part of the fructooligosaccharides is decomposed when processed as a feed and the oligosaccharide content is reduced. On the other hand, galactooligosaccharides are stable to heat and acids and can withstand processing as feed. However, there is no report on the use of galactooligosaccharides for inhibiting the growth of Salmonella. Moreover, galactooligosaccharides have been confirmed to be safe as food ingredients, and there is no danger to the human body unlike antibiotics even if they remain in the body of edible animals.
【０００４】[0004]
【発明が解決しようとする課題】上記から、人体への悪
影響が無く、かつ食用動物のサルモネラ菌を有効に阻害
する方法の開発が望まれていた。From the above, it has been desired to develop a method which does not adversely affect the human body and effectively inhibits Salmonella bacteria in food animals.
【０００５】[0005]
【課題を解決するための手段】本発明者は、上記の問題
点を解決するために研究を重ねた結果、ガラクトオリゴ
糖を有効成分とする糖混合物を動物に摂取させることに
より、動物の腸内サルモネラ菌の増殖を阻害する効果が
あることを見出し、本発明に至った。本発明で活性成分
として用いるガラクトオリゴ糖とは、乳糖に、ガラクト
ースが、β−１，４結合した三糖類である４’−ガラク
トシルラクトース及び、乳糖にガラクトース残基が１な
いし２分子結合したオリゴ糖糖である、ガラクトシルラ
クトース、ジガラクトシルラクトースを含む。Means for Solving the Problems As a result of repeated studies to solve the above-mentioned problems, the present inventors have found that a saccharide mixture containing a galacto-oligosaccharide as an active ingredient is ingested into an animal's intestine. The present inventors have found that they have an effect of inhibiting the growth of Salmonella, and have reached the present invention. The galacto-oligosaccharide used as an active ingredient in the present invention includes 4′-galactosyl lactose, a trisaccharide in which galactose is β-1,4 linked to lactose, and oligosaccharide in which one or two galactose residues are linked to lactose. It contains the sugars galactosyl lactose and digalactosyl lactose.
【０００６】本発明で用いるガラクトオリゴ糖は、業界
公知の方法、例えば、特開昭６０−２５１８９６、特開
昭６１−２３６７９０、特開昭６１−２７１９９９、特
開昭６１−２８９８５６、特開昭６２−１３０６９５な
どに示される方法にしたがって製造されたものを用いる
ことができる。[0006] The galacto-oligosaccharides used in the present invention can be prepared by methods known in the art, for example, JP-A-60-251896, JP-A-61-236790, JP-A-61-271999, JP-A-61-289856, JP-A-62 And those manufactured according to the method shown in US Pat.
【０００７】本発明に用いるガラクトオリゴ糖は、単独
でも動物に投与できるが、ガラクトオリゴ糖を有効成分
とし、他の糖質や蛋白質、ミラネル等を含有する混合物
であっても良いのは言うまでもない。The galacto-oligosaccharide used in the present invention can be administered alone to an animal, but it goes without saying that a mixture containing galacto-oligosaccharide as an active ingredient and other carbohydrates, proteins, Milanel and the like may be used.
【０００８】本発明にしたがって、ガラクトオリゴ糖を
摂取させる動物としては、食用の家畜、鶏等の鳥類だけ
でなく、サルモネラ菌が増殖する動物であれば特に制限
されない。[0008] In accordance with the present invention, the animals to be ingested with galactooligosaccharides are not particularly limited, as long as they are not only edible livestock, birds such as chickens, but also animals in which Salmonella bacteria multiply.
【０００９】本発明のガラクトオリゴ糖が阻害するサル
モネラ菌には、例えば、Ｓａｌｍｏｎｅｌｌａ ｔｙｐ
ｈｉ， Ｓ．ｅｎｔｅｒｉｔｉｄｉｓ，Ｓ．ｔｙｐｈｉ
ｍｕｒｉｕｍｅなどがある。サルモネラ菌は、大腸菌、
赤痢菌などとともにグラム陰性の通性嫌気性菌であっ
て、腸内細菌の一種である。The Salmonella bacteria inhibited by the galactooligosaccharides of the present invention include, for example, Salmonella type
hi, S.M. enteritidis, S.M. typhi
murium and the like. Salmonella is E. coli,
It is a gram-negative facultative anaerobic bacterium along with Shigella, and is a kind of intestinal bacteria.
【００１０】[0010]
【実施例】次に、実施例により本発明を更に詳細に説明
するが、本発明を限定するものではない。Next, the present invention will be described in more detail by way of examples, but the present invention is not limited thereto.
【００１１】実施例１ サルモネラ・チフィムリウム（Ｓａｌｍｏｎｅｌｌａ
ｔｙｐｈｉｍｕｒｉｕｍＩＦＯ−１４１９３）をガラク
トオリゴ糖を含むフェノールレッド肉汁ベース（Ｐｈｅ
ｎｏｌ Ｒｅｄ Ｂｒｏｔｈ Ｂａｓｅ，以下、ＰＲＢ
と略す）により次のとおり培養した。Example 1 Salmonella typhimurium
typhimurium IFO-14193) is a phenol red broth base containing galactooligosaccharides (Phe
nol Red Broth Base, PRB
).
【００１２】ＰＢＲをディフコ社（ＤＩＦＣＯ）の指示
に従って調整し、炭素源として４’−ガラクトシルラク
トースを１．０％となるように加えた。１０本の試験管
に各５ｍｌの培地を入れ、このうち５本には鉱物油を重
層し嫌気条件とした。又、対照として同組成の培地に炭
素源として１．０％グルコースを加えたものを２本用意
し、一方を嫌気条件とした。以上の各試験管は３７℃で
培養を行ない、２４、４８及び７２時間目に酸産生と菌
の増殖を測定した。これらの試験の結果を以下の表１に
示す。PBR was adjusted according to the instructions of DIFCO, and 4'-galactosyl lactose was added as a carbon source to 1.0%. 5 ml of each medium was placed in 10 test tubes, and 5 of them were overlaid with mineral oil and anaerobic conditions were set. As controls, two culture media of the same composition to which 1.0% glucose was added as a carbon source were prepared, and one of them was subjected to anaerobic conditions. Each of the above test tubes was cultured at 37 ° C., and acid production and bacterial growth were measured at 24, 48 and 72 hours. The results of these tests are shown in Table 1 below.
【００１３】表中、酸産生における「±」は弱陽性 「＋」は陽性 「＋＋」は強陽性 「＋＋＋」は最も強い陽性 「−」は陽性反応が認められない を示し、又、菌の増殖における 「±」は弱い増殖 「＋」は増殖 「＋＋」は強い増殖 「＋＋＋」は最も強い増殖 を示す。試験管６−１０及び対照２は、嫌気条件下のも
のである。 In the table, "±" in acid production is weakly positive, "+" is positive, "++" is strong positive, "++++" is strongest positive, "-" indicates no positive reaction. In the proliferation, "±" indicates weak proliferation, "+" indicates proliferation, "++" indicates strong proliferation, and "++++" indicates the strongest proliferation. Test tube 6-10 and Control 2 are under anaerobic conditions.
【００１４】その結果、本発明のガラクトオリゴ糖を与
えた場合は、対照のグルコースを加えたものに比較し
て、予期せざる優れたサルモネラ菌の増殖の阻害活性が
得られた。As a result, when the galactooligosaccharide of the present invention was given, unexpectedly excellent growth inhibitory activity of Salmonella was obtained as compared with the case where glucose was added as a control.
【００１５】[0015]
【発明の効果】本発明の方法に従えば、動物にガラクト
オリゴ糖を摂取させることにより、サルモネラ菌の増殖
が阻害され、動物を食品とした時の人間へのサルネモラ
菌の感染確率も減少されるという効果がある。According to the method of the present invention, the ingestion of galactooligosaccharides into an animal inhibits the growth of Salmonella and reduces the probability of infection of humans with Salmonella when the animal is used as food. effective.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特表 平３−501971（ＪＰ，Ａ) 「医学大辞典 第17版」（1990年２月 １日）株式会社南山堂、ｐ．743−744 「サルモネラ」の欄 (58)調査した分野(Int.Cl.7，ＤＢ名) A61K 31/7016 - 31/702 C07H 3/04 - 3/06 ＣＡ（ＳＴＮ) ＣＡＯＬＤ（ＳＴＮ) ＣＡＰＬＵＳ（ＳＴＮ) ＭＥＤＬＩＮＥ（ＳＴＮ) ＷＰＩ（ＤＩＡＬＯＧ)──────────────────────────────────────────────────続 き Continuation of the front page (56) Bibliography Reference table Hei 3-501971 (JP, A) “The 17th Edition of the Medical Dictionary” (February 1, 1990) Nanzando Co., p. 743-744 "Salmonella" column (58) Fields investigated (Int. Cl. 7 , DB name) A61K 31/7016-31/702 C07H 3/04-3/06 CA (STN) CAOLD (STN) CAPLUS ( STN) MEDLINE (STN) WPI (DIALOG)
Claims (1)
ス、ガラクトシルラクトース及びジガラクトシルラクト
ースから選ばれた１種以上のガラクトオリゴ糖を摂取さ
せることを特徴とするサルモネラ菌の増殖阻害方法。1. A method for inhibiting the growth of Salmonella, which comprises ingesting a food animal with at least one galacto-oligosaccharide selected from 4'-galactosyl lactose, galactosyl lactose and digalactosyl lactose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05446892A JP3149253B2 (en) | 1992-01-30 | 1992-01-30 | Method for inhibiting the growth of Salmonella |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP05446892A JP3149253B2 (en) | 1992-01-30 | 1992-01-30 | Method for inhibiting the growth of Salmonella |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05208912A JPH05208912A (en) | 1993-08-20 |
| JP3149253B2 true JP3149253B2 (en) | 2001-03-26 |
Family
ID=12971509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP05446892A Expired - Lifetime JP3149253B2 (en) | 1992-01-30 | 1992-01-30 | Method for inhibiting the growth of Salmonella |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3149253B2 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH056471Y2 (en) * | 1988-09-09 | 1993-02-18 | ||
| JPH03117188U (en) * | 1990-03-15 | 1991-12-04 |
-
1992
- 1992-01-30 JP JP05446892A patent/JP3149253B2/en not_active Expired - Lifetime
Non-Patent Citations (1)
| Title |
|---|
| 「医学大辞典 第１７版」（１９９０年２月１日）株式会社南山堂、ｐ．７４３−７４４「サルモネラ」の欄 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH05208912A (en) | 1993-08-20 |
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