JP3143360B2 - Ventilation equipment for the culture room - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a ventilation device in a culture room of a culture storage.

[0002]

2. Description of the Related Art In a conventional culture chamber, a filter is provided in a passage communicating between an upper part and a lower part of a culture chamber, and gas in the culture chamber is cleaned by passing gas contaminated in the culture chamber through the filter. (For example, see Japanese Unexamined Patent Publication No.
Reference).

[0003]

However, the conventional gas cleaning in the culture chamber is performed by providing a filter in a circulating gas passage and passing through the filter.
Air outside the culture chamber was not introduced, and gas contaminated by gas generated by cell metabolism remained accumulated in the room.

The present invention solves the above problem,
An air flow path provided with an air filter is provided in a seal member between the bearing portion of the fan motor and the inner box, and the negative pressure of the motor bearing portion due to the rotation of a fan provided in the passage is used to outside the culture chamber. Is introduced into the culture chamber, and the gas in the culture chamber is exhausted from the concentration detection passage in the culture chamber to ventilate the culture chamber.

[0005]

A culturing chamber for accommodating a sample such as cells, a passage communicating between the upper and lower parts of the culturing chamber, a fan disposed in the passage and an outer wall of the passage. A culturing system comprising: a blower comprising a motor for circulating gas in the culture chamber; a body having a passage for discharging gas in the culture chamber; and a supply device for supplying gas such as carbon dioxide gas to the culture chamber. In a storage, a flow path for air circulation is provided in a sealing material for sealing between a bearing portion of the motor and the outer wall. Preferably, an air flow tube made of stainless steel, copper, or the like is fitted into the sealing material as the flow path, and more preferably, an air filter is provided in the flow path for taking in outside air.

[0006]

According to the present invention having the above-described structure,
Without accumulating in the culture chamber gas contaminated by gas generated by metabolism of cells in the culture chamber, culture is performed from an air flow channel provided in a seal member that seals between a bearing portion of the motor and the outer wall. Ventilation is performed by allowing air outside the chamber to flow in and exhausting the gas inside the culture chamber from the passage for exhausting the culture chamber.

[0007]

BRIEF DESCRIPTION OF THE DRAWINGS FIG.

FIG. 1 is a perspective view of an incubator showing one embodiment of the present invention. FIG. 2 is a sectional view of the culture chamber of the present invention. FIG. 3 is an enlarged sectional view of a main part of the present invention. FIG. 4 is an enlarged cross-sectional view of a main part showing a modified example of a mounting portion of an air filter. FIG. 5 shows a gas discharge passage in the culture chamber,
This is an explanation when used for analyzing the concentration of gas in a culture chamber.

Reference numeral 1 denotes a culture chamber formed by a main body 5 filled with a heat insulating material 4 between an outer box 2 and an inner box 3 made of stainless steel, and a door 6 for opening and closing the opening of the main body. . A culture chamber 7 for culturing a sample such as cells while maintaining a predetermined temperature is formed inside the inner box 3 of the main body 5. An air jacket (not shown) for adjusting the temperature of the culture chamber 7 to an appropriate temperature is provided outside the inner box. Reference numeral 8 denotes a supply device for supplying carbon dioxide, nitrogen or the like for maintaining the inside of the culture chamber 7 at a predetermined gas concentration.

The door 6 for closing the opening of the main body 5 is a heat-insulating outer door 9.
And the inner door 10 thereof.

Reference numeral 11 denotes a duct mounted inside the inner box 3, and this duct 11 has a passage 12 formed therein. In the passage 12, a suction hole 13 for sucking gas in the culture chamber 7 is provided above the culture chamber, and a discharge hole 14 for discharging gas into the culture chamber 7 is provided below the culture chamber.

A motor 16 of the blower 15 is mounted on an outer wall 17 of the passage 12 and rotates a fan 18. The bearing portion 19 of the motor 16 and the outer wall of the passage 12
It is sealed by a sealing member 20 made of silicone foam rubber, which also has a function of absorbing vibration transmitted from the outside to the outer wall. An air flow tube 21a made of stainless steel, copper, or the like is fitted into the seal material 20 as an air flow path 21. An air filter 22 is provided on the air inlet side of the tube 21a.

The gas discharge passage 23 in the culture chamber 7 is a passage 1
2 and has an opening 23 outside the main body 5.

In the thus configured ventilator for a culture room, the temperature in the culture room 7 of the culture room 1 is maintained at an appropriate temperature by an air jacket, and the concentration of gas in the culture room 7 is controlled by the concentration of carbon dioxide, nitrogen or the like. The gas is supplied by the supply device 8 and is maintained at a predetermined concentration, and the inside of the culture chamber 7 is set to the optimal condition for culturing cells and the like.

The gas circulating in the culture chamber 7 is forcibly sucked into the passage 12 through the suction hole 13 by the fan 18, and the sucked gas is cultured through the discharge hole 14 provided below the passage 12. It is discharged into the chamber 7. At this time, when the fan 18 rotates, the shaft side of the fan 18 becomes a negative pressure, so that the air filter 22 passes through the shaft hole 24 of the motor 16 through the air passage of the tube 21 so that the passage 12
It is taken in. As a result, the air whose flow rate is restricted by the tube 21 and the air filter 22 flows into the culture chamber 7. Further, the gas in the culture chamber 7 which is contaminated with gas generated by the metabolism of the cells in the culture chamber 7 is discharged out of the culture chamber through the discharge passage 23 fitted in the passage 12. As a result, the outside air filtered by the air filter 23 is introduced into the culture chamber 7, and the gas in the culture chamber 7 is discharged from the culture chamber 1 through the discharge passage 23 through the discharge hole 25 to remove the cells. Gas generated by metabolism accumulates in the culture chamber 7 and does not inhibit the growth of the cells themselves.

The air filter 22 does not necessarily need to be provided in the flow path of the tube 21 when carrying out the present invention, and may be provided at a location remote from the tube 21a. FIG. 4 shows a modified embodiment in which a flat air filter 27 is fitted into an opening 26 of the outer wall 2 surrounding the blower 15.

As described above, the present invention not only does not require a driving part such as a dedicated fan for ventilation, but also connects the analyzer 28 to the discharge hole 24 as shown in FIG. Since the passage in which the concentration in the culture chamber 7 has been analyzed by sucking the gas in the culture chamber 7 can be used as it is, there is no need to newly provide a discharge passage 23 for discharge, which is economical.

[0018]

As described above, according to the present invention, a culture room for accommodating a sample such as a cell, a passage communicating between the upper and lower parts of the culture room, a fan disposed in the passage, and the passage A main body including a motor mounted on the outer wall of the blower for circulating gas in the culture chamber, a passage for discharging gas in the culture chamber, and a supply device for supplying gas such as carbon dioxide gas to the culture chamber. In the cultivation chamber provided with, a flow path for air circulation is provided in a sealing material that seals between the bearing portion of the motor and the outer wall, and preferably the air flow path is made of stainless steel or copper as the flow path. By fitting an air filter in the flow path for taking in outside air, more preferably, the gas in the culture chamber contains gas generated by metabolism by fitting the tube into the sealing material. It can be discharged outside the culture chamber without accumulating in the room, and the outside air filtered by the air filter can be flowed into the culture room, and a ventilation fan and a discharge passage for discharging gas for that purpose can be provided. There is no need to provide a new one, and the culture chamber can be ventilated.

[Brief description of the drawings]

FIG. 1 is a perspective view of an incubator showing one embodiment of the present invention.

FIG. 2 is a sectional view of the culture chamber of the present invention.

FIG. 3 is a sectional view of a main part of the present invention.

FIG. 4 is an enlarged sectional view of a main part showing a modified embodiment of a mounting portion of the air filter of the present invention.

FIG. 5 is a diagram when the gas discharge passage in the culture chamber of the present invention is used for analyzing the concentration of gas in the culture chamber.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Incubator 7 Culture room 15 Blower 16 Motor 17 Outer wall 18 Fan 19 Bearing part 20 Sealing material 21 Air flow path 21a tube 22 Air filter 23 Discharge path

────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Sadami Hagiguchi 2-5-1-5 Keihanhondori, Moriguchi-shi, Osaka Sanyo Electric Co., Ltd. (58) Field surveyed (Int.Cl. ⁷ , DB name) C12M 1/36-3/06

Claims (3)

(57) [Claims] 1. A culture chamber for accommodating a sample such as a cell, a passage communicating between the upper and lower parts of the culture chamber, a fan disposed in the passage, and a motor mounted on an outer wall of the passage. A blower for circulating gas in the culture chamber,
A body having a passage for discharging gas from the culture chamber,
In a culture chamber provided with a supply device for supplying gas such as carbon dioxide gas to the culture chamber, a flow path for air circulation is provided in a sealing material for sealing between a bearing portion of the motor and the outer wall. A ventilating device for a culture room. 2. A culture chamber for accommodating a sample such as a cell, a passage communicating between the upper and lower parts of the culture chamber, a fan disposed in the passage, and a motor mounted on an outer wall of the passage. A blower for circulating gas in the culture chamber,
A body having a passage for discharging gas from the culture chamber,
In a culture chamber equipped with a supply device for supplying gas such as carbon dioxide to the culture chamber, a tube for air circulation such as stainless steel or copper is used as a sealing material for sealing between a bearing portion of the motor and the outer wall. A ventilation device for a culture room, wherein the ventilation device is fitted. 3. A culturing chamber for accommodating a sample such as a cell, a passage communicating between the upper and lower parts of the culturing chamber, a fan disposed in the passage, and a motor mounted on an outer wall of the passage. A blower for circulating gas in the culture chamber,
A body having a passage for discharging gas from the culture chamber,
In a culture chamber equipped with a supply device for supplying gas such as carbon dioxide gas to the culture chamber, a flow path for air circulation is provided in a sealing material for sealing between a bearing portion of the motor and the outer wall, A ventilator for a culture room, wherein an air filter is provided in a flow path.