JP3109042B2 - Visualization judgment method of soil containing protein of plumbing products - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for visualizing and judging protein-containing dirt, and more particularly to a toilet, kitchen,
The present invention relates to a method for visualizing and judging dirt on equipment in a plumbing space such as a bathroom.

[0002]

2. Description of the Related Art Around a water space, for example, a wall of a toilet, a kitchen, a washroom, a bathroom, etc.
It is desirable that indoor sanitary facilities such as ceilings, floors, toilets, washbasins, kitchen sinks, etc., be kept clean at all times to maintain good sanitary conditions.

The walls, ceilings, floors and indoor sanitary facilities of these plumbing sanitary rooms, particularly indoor sanitary facilities, are usually cleaned at regular intervals to keep them clean. It is difficult to discriminate the dirt attached to the equipment, so that cleaning may not be performed sufficiently well and a part of the dirt may remain. In addition, since it is difficult to determine whether the dirt has been removed or not, there has been a problem that it is difficult to perform cleaning efficiently.

[0004] In this case, it is desirable that the locations where dirt is likely to occur are known in advance for each sanitary facility so that cleaning can be performed efficiently. For this purpose, a method is required that can clearly determine the presence or absence of dirt or the degree of dirt.

Conventionally, the determination of dirt on the surface of these sanitary facilities and the like has been made based on observation of the state of adhesion of water droplets and the state of wetness of water during cleaning. It is difficult to clearly determine the state of adhesion and its degree.

[0006] As a method for determining protein-containing stains, conventionally, in the food-related field, the stains on the surface of food containers and the like are scraped, and the stains are put into a reagent that reacts with the protein to form a color, and the degree of color formation is checked. A method for determining the presence or absence and degree of contamination is known in Japanese Patent Application Laid-Open No. Hei 8-21837.
issue).

Alternatively, a dye which reacts with a protein to form a color and is added with a surfactant is applied to the surface of the object to produce a color by the reaction between the dye and the protein, and stains are formed based on the color. Judgment was made so that this could be used as a target for washing with a surfactant.
No. 699.

However, since these methods all visualize protein-containing stains by coloring, the sharpness of the coloring is impaired by the color of the object, for example, in the case of a basin, the color of the basin. At the same time, there is a problem that the coloring is not sufficiently sensitive to the stain, and therefore, it is difficult or impossible to determine the stain particularly when the stain is minute.

[0009]

The invention of the present application has been made to solve such a problem. According to the first aspect of the present invention, there is provided a method for determining visualization of dirt, comprising: a wall, a ceiling, a floor, a floor , a toilet, a kitchen, a washroom, and a bathroom.
A fluorescent reagent is applied to a surface of a plumbing product containing any of the indoor facilities of the plumbing sanitary room on which dirt containing protein is adhered, and the protein is reacted with the fluorescent reagent, and then irradiated with ultraviolet light. The degree of contamination is determined by observing the fluorescence intensity from the surface of the product.

[0010] In a second aspect of the present invention, in the method for determining visualization of dirt, fluorescamine or vanillin is used as the fluorescent reagent.

According to a third aspect of the present invention, in the method for determining visualization of dirt according to any one of the first and second aspects, the fluorescent reagent is spray-sprayed on the surface of the plumbing product to react with the dirt containing the protein. And

[0012]

As described above, according to the method for determining visualization of dirt according to claim 1, the surface of a plumbing product to which dirt containing protein is adhered is irradiated with ultraviolet rays after applying a fluorescent reagent to the surface of the plumbing product. In the case of this method of visualization determination of dirt, unlike the conventional method of reacting a protein with a coloring reagent to form a dirt containing protein, the degree of dirt is determined by observing the dirt. The determination is not affected by the color of the plumbing product itself, and it is possible to detect minute dirt of about 10 ng, and it is possible to clearly determine even minute dirt.

Various fluorescent reagents can be used. In particular, fluorescamine or vanillin can be preferably used (claim 2). When vanillin is used as the fluorescent reagent, the reaction can be sufficiently performed by applying the vanillin to the surface of the object and then heating it to around 110 ° C. In the case of the former fluorescamine, the reaction can be performed at room temperature. Can react with proteins,
It is particularly suitable as a fluorescent reagent to be used.

When the fluorescent reagent is applied to the surface of the object, it is desirable to spray the fluorescent reagent on the surface of the object. In this manner, the fluorescent reagent can be easily and uniformly applied to the surface of the object over a wide range, and the surface of the object, that is, the surface of the plumbing product can be easily visualized and determined. .

In the present invention, the indoor equipment includes the following. That is, examples of indoor facilities of toilets include other toilets made of ceramics such as toilets and urinals, resin toilet lids and resin toilet seats attached to the toilets, and other hand washers made of ceramics and washing water tanks. The kitchen facilities include kitchen sink,
Water faucet fittings, ceramic tiles constituting kitchen walls, kitchen counters and the like can be mentioned.

Further, examples of the indoor facilities of the washroom include other basins and counters made of ceramics, and examples of the indoor facilities of the bathroom include a bathtub, a bathroom counter, and a faucet fitting. In addition, a ceramic tile or the like constituting a wall or the like of each plumbing sanitary room is regarded as one plumbing product, and the stain on the surface can be determined by causing the fluorescent reagent to emit fluorescent light according to the present invention.

[0017]

Next, embodiments of the present invention will be described in detail. <Example 1> and protein (including -NH ₂₎ corresponds substance nutrient broth (Eiken Chemical Co., Ltd.) 18 g was dissolved in distilled water 1000ml as in stock as soil components, the stock solution and this 1 / 0.5, 1/500 and 1/1000 dilutions were inoculated in 0.5 ml portions on the surface of a test piece made of sanitary ware and dried.

The composition of the stock solution is as follows per 1000 ml. Stock comprising extract 3g Peptone 10g sodium chloride 5g this meat extract, peptone, sodium chloride is a substance that contains -NH _2.

Next, the above stock solution, 1/50 diluent, 1/50
Fluorescamine and ninhydrin were sprayed on the surface of the test piece inoculated with the 0 dilution and the 1/1000 dilution. In addition, what sprayed ninhydrin was heat-processed at 110 degreeC after that for 10 minutes.

Here, fluorescamin
e) is a fluorescent reagent represented by the chemical formula of C ₁₇ H ₁₀ O ₄ (it does not emit fluorescence by itself), and as shown in the following formula (1), a protein (specifically, an amino group of a protein, Reacts with the amino group of the primary amine) to produce a fluorescent substance.

[0021]

Embedded image

In this example, 0.025 g of fluorescamine was used as the solvent in dimethyl sulfoxide 10
The solution dissolved in 0 ml was used. The spray amount on the surface of the sample was 0.01 to 0.20 g per 1 cm ² . A desirable spray amount is 0.03 to 0.15 g, and a more desirable spray amount is 0.03 to 0.12 g.

After the fluorescamine and ninhydrin are sprayed as described above, the ultraviolet rays (wavelength 36
5 nm), and the state of the sample surface was observed.
As a result, the fluorescamine sprayed showed fluorescence and the fluorescence intensity was strong in the order of 1/1000 diluted solution → 1/500 diluted solution → 1/50 diluted solution → stock solution. It should be noted that ninhydrin reacts with proteins to develop color and does not generate fluorescent substances.

With respect to the ninhydrin-treated solution, only the solution inoculated with the undiluted solution and the 1/50 dilution without irradiation with ultraviolet light produces a color that can be discerned with the naked eye, and the degree of the color development is naturally inoculated with the undiluted solution. Is strong,
The degree of color development was weak for the 1/50 dilution. No color development was observed with the naked eye for the inoculated 1/500 diluted solution and 1/1000 diluted solution.

Tables 1 and 2 show the results. Here, Table 1 shows the results of visual confirmation without irradiation with ultraviolet rays, and Table 2 shows the results of visual confirmation with irradiation of ultraviolet rays.

[0026]

[Table 1]

[0027]

[Table 2]

In this test, the surface state of the fluorescamine and ninhydrin which were not spray-treated (non-spray-treated product) was similarly observed. / 50 diluent, 1/500
Regarding the diluent and the 1/1000 diluent, it was not possible to sufficiently confirm that the surface state changed depending on the degree of dirt (the amount of inoculated broth).

As is clear from the results shown in Tables 1 and 2, in the method of judging dirt by spraying ninhydrin to form a color, when the degree of dirt is reduced, this is clarified. Although it cannot be visually confirmed, in the case of the method of the present embodiment in which fluorescamine is sprayed and irradiated with ultraviolet rays to cause the sample surface to emit fluorescent light to determine the degree of dirt, even if the degree of dirt is slight, it can be clearly determined. Can be visually confirmed and determined.

<Example 2> A basin bowl of an existing men's toilet was treated according to the following conditions, and stains adhering thereto were investigated using fluorescamine as a fluorescent reagent. Specifically, half of the existing basin bowl (left half) is washed under the following conditions, and the other half (right half) is left as it is, spraying fluorescamine and irradiating with ultraviolet light. The photograph was taken and the state of dirt was observed. The basin bowl has been estimated for about 10 hours since the last cleaning.

[Test conditions] Target wash basin bowl Wash basin bowl with office men's toilet (approximately 1 after cleaning)
0 hours) ・ Pretreatment of spraying fluorescamine The left half of the bowl is washed with a neutral detergent and tap water using a nylon scrubber → rinse with distilled water → dry with a dryer → use tap water with a faucet equipped with a basin. Rinse → Rinse with distilled water → Dry with dryer ・ Spraying fluorescent reagent Fluorescamine in dimethyl sulfoxide (0.2
(5 g / liter) was sprayed uniformly over the entire inner surface of the basin bowl with a sprayer, and allowed to stand for 30 minutes to perform fluorescence imaging. -Fluorescence photography Illumination: 365nm ultraviolet light source side filter: ultraviolet cut filter Camera side filter: ultraviolet cut filter Camera used: Olympus OM-2 Exposure time: 1 minute Aperture: 3.5

As a result, the right half of the basin bowl that had not been subjected to the cleaning treatment was entirely stained, had poor surface gloss, and repelled water. After applying the fluorescamine solution, the surface condition was photographed under ultraviolet irradiation. As a result, the right half of the basin bowl was strongly fluoresced, and the photograph was whitish.
That is, after applying fluorescamine, taking a photograph under ultraviolet irradiation, the state of dirt could be clearly seen.

In Examples 1 and 2, fluorescamine can of course be used in combination with other solvents other than the above examples or at different concentrations. For example, it may be used in a combination of fluorescamine 0.025 g / dimethylformamide 100 ml, or fluorescamine 0.050 g / dimethylsulfoxide 100
fluorescamine 0.050g
/ Dimethylformamide 100 ml combination or other combinations.

Here, 0.025 g of fluorescamine was used.
Dimethyl sulfoxide in 0.050 g, twice the amount of
When used by dissolving in 100 ml of a solvent such as dimethylformamide, a sufficient amount of fluorescamine that reacts with dirt is supplied even when the spray amount on the surface of the object is small, so that a sufficient reaction can be obtained. .

Here, no problem arises even if fluorescamine is supplied in excess to the surface of the object. This excess of fluorescamine that has not been used in the reaction can be easily hydrolyzed to form a water-soluble non-fluorescent substance, which can be discharged together with water.

When a solvent that has been subjected to a non-fluorescent treatment (does not emit fluorescence even when irradiated with ultraviolet rays or the like) is used as the solvent, the fluorescence due to the reaction with fluorescamine is more remarkably advantageous.

In the above embodiment, the ultraviolet wavelength is 365 n
m, but 360, 3
The wavelength may be changed near 360 nm, such as 65,366 nm, or a wavelength of 254 nm or near may be used.

Although the above is an example of the case of fluorescamine, the present invention can also use vanillin as a fluorescent reagent. That is, the present inventor sprayed and sprayed this using vanillin as a fluorescent reagent, and then performed a test in which the reaction was heated to about 110 ° C. and then irradiated with ultraviolet light to examine the fluorescent intensity. It could be confirmed visually.

The embodiment of the present invention has been described in detail above, but this is merely an example. For example, in photography, it is also possible to attach a filter that transmits only the fluorescence wavelength to the camera and take a picture. In this case, the resulting photo shows the fluorescent part but most of the sample is reflected by adjusting the exposure time. Since it is possible to create a situation in which the fluorescent portion is not present, it is possible to further enhance the fluorescent portion. In addition, the present invention digitizes the fluorescence emission from the surface of the object by image processing, and it is also possible to judge dirt based on the numerical value. It is feasible.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Shungo Tokushima 5-1-1 Koiehonmachi, Tokoname-shi, Aichi Prefecture Inax Corporation (72) Inventor Hiroyuki Miyamoto 5-1-1 Koiehonmachi, Tokoname-shi, Aichi Stock Company Inax Corporation (72) Inventor Meiko 5-1-1 Koiehonmachi, Tokoname-shi, Aichi Pref. Inax Corporation (56) References JP-A-61-164158 (JP, A) Japanese Utility Model Application Hei 5-24043 (JP) , U) Biochemical Dictionary (Second Edition), p. 1150 "Fluoresamine"

Claims (3)

(57) [Claims] [Claim 1] toilet, kitchen, washroom, one of the bathroom
The wall, ceiling, floor of the plumbing room and the plumbing room
A fluorescent reagent is applied to a surface of a plumbing product including any of indoor facilities to which dirt containing protein is attached, and the protein is reacted with the fluorescent reagent, and then irradiated with ultraviolet light, and the fluorescence intensity from the plumbing product surface is irradiated. A visualization determination method for dirt containing protein of a plumbing product, wherein the degree of dirt is determined by observing water. 2. The method according to claim 1, wherein fluorescamine or vanillin is used as the fluorescent reagent. 3. The plumbing product according to claim 1, wherein the fluorescent reagent is spray-sprayed on the surface of the plumbing product to react with the dirt containing the protein. Soil visualization determination method.