JP3063985B2 - Disposable inspection tool - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a disposable test device suitable for measuring the number of sperm in semen, the number of red blood cells and the number of white blood cells in blood, and the like.

[Prior Art] In the treatment of obstetrics and gynecology infertility, there is a step of measuring the number of sperm present in a unit volume of semen. In a conventional sperm count measuring instrument, after semen is dropped into a transparent container, a cover glass is attached to the container, and the depth of the container is fixed to, for example, 10 μm. Grid lines with 0.1mm intervals are written on the cover glass. A microscope (eyepiece 10x,
Observe the sperm with an objective lens (20x), count the number of spermatozoa present in the 10 grids, multiply this sperm count by 1 million, and
The number of sperm present in ml of semen is calculated.

[Problems to be Solved by the Invention] However, the above-mentioned conventional sperm count measuring instrument includes the following:
There is a problem.

After dropping the semen into the container, it is necessary to apply a cover glass, and the operability is poor.

The structure is complicated, it cannot be inexpensive enough to be disposable, and lacks hygiene due to repeated use.

An object of the present invention is to provide a sanitary disposable inspection tool with good operability.

[Means for Solving the Problems] The present invention according to claim 1 is a disposable inspection tool for microscopic observation for inspecting an object to be inspected in a test solution while the test solution is contained in a test solution storage space. The inspection tool comprises a plastic upper transparent plate, a lower transparent plate, and a lower transparent plate located between the upper transparent plate and the lower transparent plate, and a plurality of upper and lower transparent plates bonded together. An inspection tool comprising a double-sided adhesive tape for forming a divided test solution storage space, wherein the test solution storage space of the test tool has a space corresponding to the thickness of the double-sided adhesive tape, The inspection tool comprises a plurality of discontinuous cutouts located at an upper portion of the lower transparent plate and provided at one end of the upper transparent plate, and communicates with the plurality of test solution accommodating spaces individually. It has a plurality of areas under test droplets that are independently divided, and The plurality of areas under test droplets are divided by an upper transparent plate located between the notches and a double-sided adhesive tape located between the upper transparent plate and the lower transparent plate, and The dropped test solution enters the respective test solution storage spaces by capillary action.

According to a second aspect of the present invention, in the first aspect of the present invention, grid lines are formed at regular intervals in a portion corresponding to the test solution accommodation space of one of the upper and lower transparent plates. .

According to a third aspect of the present invention, in the first or second aspect of the present invention, the upper transparent plate further has a writable print coloring portion.

The invention according to claim 4 is the invention according to any one of claims 1 to 3, wherein the inspection tool is located above the lower transparent plate and the other end of the upper transparent plate. Comprises a plurality of non-continuous notches provided in the plurality, the plurality of test liquid drop areas are respectively communicated with the plurality of test solution storage space, and the plurality of test drop lower areas are located between the notches It is made to be divided by an upper transparent plate.

According to the first aspect of the present invention, when the test solution is dropped onto the area under the test liquid drop, the test solution penetrates into the test solution storage space between the upper and lower transparent plates by capillary action, and the test solution storage space. Expands into a space of a certain depth determined by. Then, the test object in the test solution is observed with a microscope, and a specific number of grids formed on one of the upper and lower transparent plates as described in claim 2, or a specific number of equally divided eyepiece micrometer of the microscope By counting the number of test objects existing in the grid of the above, it is possible to calculate the number of test objects existing in a test solution of a specific volume.

 Therefore, according to the present invention, the following effects are obtained.

Simply by dropping the test solution into the region below the test droplet, the test solution can be spread by capillary action to the test solution storage space having a certain depth formed between the upper and lower transparent plates. Therefore, operability is good.

It has a simple structure, is inexpensive enough to be disposable, and is hygienic.

After the test solution is stored in the test solution storage space, petrolatum or mineral oil (sulfurized paraffin) is dropped into the area under the test liquid droplets, and the test solution is dried if the opening of the test storage space is closed. It is possible to obtain a raw specimen which can be sealed without performing the measurement and can be measured for a long time.

Further, by setting the number of sections of the test solution accommodating space provided in one test tool to be plural, a large number of samples can be observed and measured by one test tool.

Example FIG. 1 is a plan view showing a disposable inspection tool according to a first embodiment, FIG. 2 is a cross-sectional view of FIG. 1, and FIG. 3 is a plan view showing a disposable inspection tool according to a second embodiment. It is.

The disposable inspection tool 10 is, as shown in FIGS.
The upper and lower transparent plates 11 and 12 are stuck together via a double-sided adhesive tape 13 having a constant thickness (20 μm) to form a test solution accommodating space 14 with a constant depth (20 μm) between the upper and lower transparent plates 11 and 12. On the upper part of the lower transparent plate 12, there is provided a lower region for a test droplet formed by partially cutting the upper transparent plate 11 in a semicircular shape.

In addition, the inspection tool 10 includes three independent test solution accommodating spaces 14, and a test liquid dropping region 15 for the test solution accommodating space 14 at two locations on both sides of each test solution accommodating space 14. I have to do that. Specifically, as shown in FIG. 1, the test tool 10 includes a plurality of test solution accommodation spaces, is located above the lower transparent plate 12, and is provided at one end of the upper transparent plate 11. A plurality of (three) notched portions that are not continuous. Then, as shown in FIG.
A plurality of test droplet sub-regions which are separately communicated with each other and which are individually communicated with the plurality of test solution storage spaces are formed. In addition, the plurality of lower areas of the test droplets are located on the upper transparent plate 11 located between the notches 14.
And a double-sided adhesive tape 13 located between the upper transparent plate 11 and the lower transparent plate 12. In addition, in the inspection tool 10,
As shown in FIG. 1, it is located above the lower transparent plate 12 and
The other end of the upper transparent plate 11 is also formed with a plurality of (three) notches that are not continuous. As shown in FIG. 1, the notch portion also forms a plurality of test liquid drop regions separately communicated with the plurality of test solution accommodating spaces. The area under the droplet is separated by an upper transparent plate located between the notches.

In addition, by changing the size and division of the test tool 10 and the test accommodating space 14, one to many samples can be observed and measured with one test tool 10.

At this time, in the inspection tool 10, only the area corresponding to the test solution accommodating space 14 of the upper transparent plate 11 is a transparent portion, and the remaining portion is a writable print coloring portion. In addition, inspection tool 10
Indicates that the lower transparent plate 12 is entirely transparent.

As a material of the lower transparent plate 12, in addition to glass, polycarbonate, polystyrene (PS), polyvinylidene (PVD
C), polyimide, polyamide, nylon, silicone,
Plastics such as polyvinyl chloride (PVC), polyurethane (PU), polyethylene (PE), polypropylene (PP), and polyester are suitable.

Further, as a material of the upper transparent plate 11, polycarbonate,
Polystyrene, polyvinylidene (PVDC), polyimide,
Polyamide, nylon, silicone, PVC,
Plastics such as polyurethane, polyethylene, polypropylene and polyester are preferred.

Further, the smaller the thickness of the upper transparent plate 11, the higher the magnification of the microscope. The thickness of the upper transparent plate 11 this time is 0.8m
m, using an eyepiece 10x and an objective 40x, 400
Double observation was made possible.

Further, the inspection unit 10 includes one of the upper and lower transparent plates 11,
Grid lines 16 having a constant interval (0.1 mm) are formed in a portion corresponding to the twelve test solution accommodation spaces 14. The grid lines 16 can be formed by writing, microfilm technology, or the like.

When the grid lines 16 are formed by the microfilm technique, a gelatinous photosensitive agent is applied on the inner surface of the lower transparent plate 12, and then the photosensitive agent is printed with the grid lines. At this time, if the grid lines 16 are to be formed on the inner surface of the upper transparent plate 11, the extremely rough surface of gelatin remaining on the inner surface of the upper transparent plate 11 is not appropriate because it hinders optical observation.

 Next, the operation of the first embodiment will be described.

According to the test tool 10, for example, when semen is dropped into the test liquid drop area 15, the semen penetrates into the test liquid storage space 14 between the upper and lower transparent plates 11, 12 by capillary action, and this test liquid storage space is determined. Spread to a certain depth space.

Then, by observing the sperm in the semen with a microscope, by counting the number of spermatozoa present in a specific number of grids formed on one of the upper and lower transparent plates 11, 12, in the semen of a specific volume The number of sperm present can be calculated. When the depth of the test solution accommodating space 14 is 20 μm and the interval between the grid lines 16 is 0.1 mm, the number of spermatozoa in the five grids is counted.
Multiply by 10,000 and observe the number of sperm present in 1 ml of semen.

Therefore, according to the above embodiment, the following effects are obtained.

By simply dropping the test solution into the test liquid drop area 15, the test solution can be spread by capillary action to the test solution storage space 14 having a certain depth formed in the upper and lower transparent plates 11 and 12. Therefore, operability is good.

It has a simple structure, is inexpensive enough to be disposable, and is hygienic.

After the test solution is stored in the test solution storage space 14, the area under the test droplet is
Vaseline or mineral oil (sulfurized paraffin) for 15
Is dropped to close the opening of the test solution accommodating space 14, the test solution can be sealed without drying, and a live specimen that can be observed for a long time can be obtained.

Double-sided adhesive tape for bonding upper and lower transparent plates 11 and 12
13 also functions as a space setting member between the transparent plates 11 and 12. Therefore, the presence of the double-sided adhesive tape 13 is
Is simplified, the assemblability is improved, and the disposable use of the inspection tool 10 can be promoted.

3 differs from the above-described test tool 10 in that the grid lines 16 are not formed in the test solution accommodating spaces 14 of the upper and lower transparent plates 11 and 12. When this inspection tool 20 is used, a grid line of an equally divided eyepiece micrometer of a microscope can be used instead of the grid line 16.

The test tool of the present invention is useful not only for observing sperm in semen but also for various tests such as the above (1) to (3).

(1) Blood test method Blood is used as a test solution, and red blood cells or white blood cells are used as test subjects.
Estimating the number of red blood cells or white blood cells in blood (2) Urine sediment test method Using urine sediment as a test solution, using red blood cells or white blood cells as the test object, and calculating the number of red blood cells or white blood cells in urine sediment (3) Staining method of blood or urine sediment The blood or urine sediment in blood or urine sediment can be observed by coating the inner surface of the upper and lower transparent plates forming the test solution accommodating space with a staining agent in advance.

For example, in order to test for reticulocytes, which are juvenile types of red blood cells, if a new methylene blue stain is coated on the inner surface of the test solution storage space, the test solution dissolves the stain and stains the reticulocytes. You. Then, the reticulocytes can be calculated.

In addition, in order to examine squamous epithelial cells, columnar epithelial cells, macrophages, malignant cells, etc. in urine sediment, if the stain of Sternheimer staining is coated on the inner surface of the test solution storage space, the test can be performed. The liquid dissolves the stain and the cells are stained.
Then, the classification and the number of these cells can be calculated.

[Effects of the Invention] As described above, according to the present invention, it is possible to obtain a sanitary disposable inspection tool with good operability.

[Brief description of the drawings]

FIG. 1 is a plan view showing a disposable inspection tool according to the first embodiment, FIG. 2 is a cross-sectional view of FIG. 1, and FIG. 3 is a plan view showing a disposable inspection tool according to the second embodiment. 10, 20 …… Disposable inspection tool, 11, 12 …… Transparent plate, 13…
... double-sided adhesive tape, 14 ... test solution storage space, 15 ... area under test droplet, 16 ... grid lines.

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int.Cl. ⁷ , DB name) G01N 1/28 G01N 33/49-33/50

Claims (4)

(57) [Claims] 1. A disposable inspection tool for microscopic observation for inspecting an object to be inspected in a test solution in a state where the test solution is accommodated in a test solution storage space, wherein the inspection tool comprises a plastic upper transparent plate. A lower transparent plate and a double-sided adhesive tape for forming a plurality of divided test solution accommodation spaces between the upper and lower transparent plates while laminating the upper and lower transparent plates between the upper transparent plates. An inspection tool, wherein the test solution accommodating space of the inspection tool includes a space corresponding to the thickness of the double-sided adhesive tape, and the inspection tool is located above the lower transparent plate and the upper transparent plate. A plurality of non-consecutive cutouts provided at one end of the plate, comprising a plurality of test liquid drop areas separated independently from each other and individually communicating with the plurality of test solution storage spaces, and The lower area of the test droplet is an upper transparent plate located between the notches. It is separated by a double-sided adhesive tape located between the upper transparent plate and the lower transparent plate, and the test solution dropped in the lower area of each test liquid drops enters each of the test solution accommodating spaces by capillary action. A disposable inspection tool for microscopic observation, which is characterized in that: 2. The disposable test tool according to claim 1, wherein grid lines are formed at regular intervals in a portion corresponding to the test solution storage space of one of the upper and lower transparent plates. 3. The disposable inspection tool according to claim 1, wherein the upper transparent plate has a writable print coloring portion. 4. The test tool comprises a plurality of discontinuous cutouts located above the lower transparent plate and provided at the other end of the upper transparent plate. A plurality of test droplet sub-areas each communicating with each other, and
The disposable inspection tool according to any one of claims 1 to 3, wherein the plurality of lower areas of the test droplet are separated by an upper transparent plate located between the notches.