JP3057676B2 - Inoculation method of mycorrhizal fungi - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a method of inoculating mycorrhizal fungi with a seed.

Mycorrhizal mushrooms grow mycelia while ingesting nutrients and the like necessary for mycelial growth from the roots of the tree, which is the host, and the present invention relates to edible mycorrhizal fungi of high economic value such as matsutake and hon-shimeji mushrooms. The present invention relates to a method for inoculating a root of a host tree during inoculation of mushrooms.

[0003]

2. Description of the Related Art In the prior art in the art, a method has been adopted in which a fine root portion or a site where a fine root is overgrown in soil is selected and a seed fungus is inoculated into a host tree at random. This method has been adopted because of the following three remarkable properties of mycorrhizal fungi.

[0004] First, putrefactive mushrooms such as cellulose, hemicellulose, and lignin, which are abundantly present in forest soil, are easily taken as macronutrients by mycorrhizal fungi as macronutrients. Second, the main nutrients for the growth of mycorrhizal mushrooms are limited to specific low-molecular carbohydrates, which are abundant especially in the fine roots of the host tree. The mycelium is characterized by the fact that the growth of mycelia is much slower than that of decay fungi, and the mycelia only grow at the terminal fine roots of the roots of the host tree.

[0005] However, in this method, fine roots or sites of overgrowth of fine roots disappear or move with the growth of the host tree and are newly formed.
After 2 years, the mycorrhizal roots are usually devolatilized or extinguished. It is difficult to form large white soil, that is, a mycelial mass of soil in the soil that is the basis of fruiting body development. At the time of inoculum inoculation, different types of fungi have already formed mycorrhizas in the inoculation site, fine roots or overgrown sites, and often coexist with the host tree, and such different types of fungi can form mycorrhizas of the fungi to be cultivated. There is also a problem that it may inhibit the formation of a white body in a sufficient amount and in a wide range for fruiting body formation.

[0006] To solve such a problem to a considerable extent,
This is a technique of Japanese Patent Application No. 4-93743 according to the invention of the present inventor. According to this technique, as shown in FIG. 6, the root of a host tree is cut, and the cut portion is inoculated with a seed fungus. The soil is low in the content of mycorrhizal fungi that is not to be cultivated. The roots of the mushrooms to be cultivated are coated on the fine roots of the cultivated bacterial species, and a new fungus which inhibits the implantation of the cultivated bacterial species and the formation of mycelium is not implanted. Are used to form hyphae.

[0007]

[Problems to be solved]
In the technique of No. 743, depending on the size of the host tree, the site of the root to be cut, and the root diameter, for example, a root diameter of 4 to 10 c
When the inoculum is inoculated by cutting the main or branch root of m
It can degrade and sometimes die the host tree.

[0008] If such root cutting work is continued for several years, the formation of the root that maintains the uprightness against the elongation of the tree height is not accompanied, and this may cause the tree to fall. In addition, as the trees grow, the fine roots grow farther away than the woods, and the routes of transport of nutrients and water increase accordingly, the metabolic burden on the trees increases, the tree vigor decreases, and the trees age. It will be easier.

[0009] In addition, if the roots that support the erecting of the host tree are inadvertently cut, it will be in a fragile erect state, increasing the risk of falling in strong winds. There has been a problem that a great deal of labor is required for the work of locating the cutting root to avoid it and the cutting work itself.

In view of the above problems, the present invention does not require a large amount of labor, incurs little decrease in the vigor of a host tree, inoculates mycelial fungi at the time of inoculation of mycorrhizal fungi to be cultivated. It is an object of the present invention to provide a method for inoculating mycorrhizal fungi with inoculation of mycorrhizal fungi such that mycorrhizal formation is sure and a white, that is, a soil hypha layer is formed smoothly.

[0011]

DISCLOSURE OF THE INVENTION The present invention provides a method for digging a ground on which a tree hosting mycorrhizal mushrooms is vegetated, exposing the root of the host tree, and inoculating the root of the host tree with mycorrhizal fungi. After inoculation of the inoculum, mycorrhizal mushrooms that are covered with soil with a low mycorrhizal fungi content, for example, mountain soil that does not contain a humus layer, are excavated for inoculation and exposing the roots In the inoculum inoculation method, a notch portion having a constant width or an arbitrary shape is pierced from the root surface to the root portion of the host tree, and the notch portion is a notch from the epidermis portion to the woody portion. Along with maintaining a certain part of the woody part without notching it, as a means for solving the main problem, inoculating mycorrhizal mushroom inoculum on or above the notch,
The mycorrhizal fungi to be cultivated by this means for solving the main problem can be applied to many types, and among them, Tricholoma genus and Lyophyllum which are important for forest resources are
For the genus Tricholoma, especially Tricho
loma matsutake (Japanese matsutake), Tricholoma bakam
The atsutake species (Japanese name Bakamatsutake) is, in the latter genus Lyophyllum, particularly the Lyophyllum shimeji species (Japanese name Shimeji or Honshimeji) and the Lyophyllum fumosum species (Japanese name Shakashimeji).

[0012] In the means for solving the above problems, among mycorrhizal fungi to be cultivated, Tricholoma using a conifer as a host is used.
The mycorrhizal fungi to be cultivated, excluding the matsutake species, are especially used as host trees in the family Fagaceae, Lyophyllum shimeji, Lyophyllum fum
osum also uses ilex trees as host trees. However, Lyophy
Hyphal implantation of llum shimeji on ilex trees is Ly
Inferior to ophyllum fumosum. Pine conifers are also available from Tricho
In addition to loma matsutake species, Lyophyllum shimeji species,
It is a host tree of mycorrhizal fungi to be cultivated by the genus Tricholoma and Lyophyllum.

In the present invention, as an auxiliary means for solving the above problems, cultivated mycorrhizal mushrooms such as Ly
At the time of cultivation of ophyllum fumosum species, ilex tree is arranged at an appropriate position between the trees of the host tree as a means for expanding the hyphae bed.

[0014]

According to the above-mentioned structure, first, a notch of a certain width all around, or an arbitrary shape, from the epidermis to the woody part is cut from the root surface to the root of a tree serving as a host of mycorrhizal fungi. Cutting, inoculating mycorrhizal fungi in the above-mentioned notch or its vicinity while preserving it without cutting out a certain part of the woody part, especially a part that may seriously affect the vital activity of the root. . In other words, a certain part of the root is cut from the cortex to the cambium without cutting, and the inoculum is inoculated on or near the wound.

In the cut-out portion, the roots of the fine roots become active.
Abundant supply of specific low-molecular weight carbohydrates, which are nutrients for the growth of mycorrhizal mushrooms, and the fact that fungi that inhibit the growth of the mycelium of the mushrooms to be cultivated can cause the unimplanted state to appear at any time. As a result, implantation and growth of mycelia of mycorrhizal mushrooms to be cultivated can be ensured, and formation of white soil, that is, a mycelial soil mass in the soil, can be facilitated.

Since the cut-out portion of the root can be arbitrarily selected, the overgrowth of the fine root can be formed at an arbitrary position in the region where the root is buried. Therefore, it is possible to form the end of the root, which is apt to be more distant from the xylem, especially the fine root or the fine root vegetation, at an arbitrary position within a range close to the xylem without being greatly affected by the growth of the host tree. .

In this way, a large amount of fine roots can be generated at an arbitrary position in a range close to the tree part, so that the difficulty of implantation and growth of hyphae due to the disappearance of the movement of the fine root or the fine root growing position is dramatically reduced. However, a large white of mycorrhizal fungi to be cultivated can be formed in the vicinity of the tree part of the host tree. As a result, the incidence of cultivated fungal seed bodies is also improved, and the fruit body yield is also increased.

By taking the above measures to solve the above-mentioned problems, cutting of the host tree becomes unnecessary, and fine roots or thick root growing parts are scattered in the far area of the tree part, which are observed after considerable growth of the tree. In comparison with water, the upstream distance of nutrients is shorter, efficient metabolism is performed in the host tree, and as a result, the progression of aging of the host tree is slowed, so that the vigor is not reduced, and Since the erecting of the xylem is not weakened, it is possible not only to prevent the tree from withering and falling, but also to use the same host tree repeatedly for a long period of time.

In addition, the inoculation of the mycorrhizal fungi to be cultivated is limited to the vicinity of the tree part, and it is not necessary to cut the roots. Labor saving can be achieved.

The mycorrhizal fungi to be cultivated are Tricholo, which has many marketable fungal species as edible mushrooms.
Ma and Lyophyllum, but the former Tricholo
In the genus ma, Tricholoma matsutake (Japanese matsutake) mainly uses Pinus densiflora as a host tree,
loma bakamatsutake (Japanese name bakamatsutake) is Mizunara,
The host tree is a beech family tree such as oak, oak and citrus. In the latter genus Lyophyllum, Lyophyllum s
The himeji species (Japanese name Shimeji or Hon-shimeji) use trees of both Pinaceae and Fagaceae, while the Lyophyllum fumosum species (Japanese name Shakashimeji) prefers Fagaceae trees, but do not use Pinaceae trees. However, these two species of the genus Lyophyllum also use ilex trees such as soyogo as host trees.
However, the mycelial implantation growth of Lyophyllum shimeji on ilex trees is inferior to that of Lyophyllum fumosum. As described above, the host tree of mycorrhizal mushrooms has specificity, and the means for solving the above-mentioned problems is also applied to such a host tree.

For example, in the case of Japanese red pine, which is a host tree of Tricholoma matsutake species (Japanese matsutake), cutting a thick root of 30 years or older causes the possibility of falling off, so that all around the root is damaged. Instead, it is preferable to leave a part that is not damaged, and the effect of preventing tree vigor reduction by the means for solving the above problems is great.

In the case of large trees such as oaks, oaks, and citrus which are beech family trees, the main root of the root becomes very thick. If the roots are cut, there is a risk of falling if there is a tree in the sandy layer, and if it takes a lot of effort to cut the thick roots, the above-mentioned means of solving the problem will make the top and side surfaces of the roots It is easy to injure and inoculate the inoculum, and it has a large fall prevention effect as compared with the prior art.

Even in the same beech family tree, in the case of oaks and soybeans, which are ilexaceae trees, the roots are not much thicker than those of the above tree species such as red pine, oaks, oaks and shii. There is a property that the root does not go deep inside. Therefore, as in the conventional example, when these thick roots are cut, it becomes very easy to fall down, so if the entire wood around the roots is damaged by the above-mentioned means for solving the problems of the present invention and the internal woody portion is left, the fall prevention effect, Since the unimplanted state of fungi other than the fungal mycelium to be cultivated can be revealed at any time near the ground surface, white formation near the ground surface is promoted, and the yield of fruiting bodies generated on the ground surface is promoted. Is also expected to increase.

However, in the case of ilex trees, it seems that the white formation is slightly inferior to that of the beech trees. Accordingly, it is needless to say that oaks have utility value as host trees, but the iridaceous trees such as soyogo are located at the appropriate positions between the trees of the beech family host trees such as red pine, oaks, oaks and cypresses. In addition, if it is arranged as a means for expanding the hyphae bed, it has a great effect on the formation of whites.

[0025]

BRIEF DESCRIPTION OF THE DRAWINGS FIG.

(First Example) A 36-year-old Japanese red pine root was inoculated with Tricholoma matsutake inventor's laboratory stock M-03 in the spring of April. The inoculum used in this example was obtained by culturing the mycorrhizal fungi according to the invention of the present inventors. Japanese red pine, which is a host tree as a group in which the present example was performed (hereinafter referred to as an “exemplary group”), has a diameter of 3 to 5 cm corresponding to a main root among those vegetated on similar terrain in terms of sunlight, drainage, inclination, and the like. Five trees having at least two thick roots are selected, two roots suitable for inoculation are selected at a point 1 m from the host tree body, a vegetation surface E is excavated, and these are inoculated with inoculation. went.

In the inoculum inoculation method of the present embodiment, first, a wedge-shaped notch, ie, a wound, adapted to the size of the inoculum using a saw or a sword on the upper surface of the root 1 to be inoculated and the three sides on both sides. Is provided. As shown in FIG. 1 which is a schematic disassembled end view of the root portion of the inoculum, the cutouts 1a to 1c are cuts having a depth of about 1/3 of the root diameter at the deepest part of the wound. The wedge-shaped portions 1d to 1f are cut off or deleted, and as shown in FIG. 2 which is a schematic plan view of the inoculum-inoculated root portion, 30 to 50 wedges are formed in the wedge-shaped wounds 1a to 1c.
Seed mass 2 of cm ³ was placed and inoculated, and the inoculated site was covered with soil having a low mycorrhizal fungi content, ie, mountain soil S containing no leaf layer at all, as shown in FIG. .

On the other hand, the control group is based on the technique according to Japanese Patent Application No. 4-93743, which is one of the conventional methods of inoculating mycorrhizal fungi.
That is, in short, a root cutting method is adopted, and five host trees, Pinus densiflora and vegetative roots having almost the same vegetation state as those of the working group are selected, and the roots of the two roots are shown in FIG. And then inoculated with the seed fungus mass 12 having the same size of 30 to 50 cm ^{3 on} the cut surface 11 at the same time as the working group, and inoculated with the inoculated site in soil having a low mycorrhizal fungi content, that is, no mulch. It was covered with mountain soil S containing no layer.

As described above, after the inoculum inoculation procedure was completed for both the experimental group and the control group, the number of wilts of Japanese red pine, which is the host tree of both the experimental group and the control group, was observed in March of the following year. Three of the five plants were found to die, whereas the number of withers in the experimental group was zero.

At the time of the above-mentioned number of withering observations,
As a result of investigating the root inoculation sites and the mycorrhizal formation of the new roots in the control group and the roots of the inoculum of both groups, as shown in FIG. 2-5
The roots of the new roots 3 of the book were observed, the elongation of these new roots was good, and remarkable mycorrhizalization was recognized. On the other hand, in the control group, as shown in FIG. New root 13 from department
Rooting was as low as zero or 1-2, and only poor mycorrhizal findings were obtained.

Next, the results of observation of the mycorrhizal formation of Matsutake fungus, which is the bacterium to be cultivated, show that in the working group, the fine roots 4 at 1-2 cm from the tip of the new roots 3 in any of the host trees. A large number of branches are remarkably observed, and the fine roots 4 are observed to be discolored to black-brown, which is peculiar to the implantation of matsutake fungi and mycorrhiza formation. Was remarkably entangled in tufts. On the other hand, in the control group, when the withered host tree was similarly observed, although the rooting of one or two new roots 13 was observed as described above, as shown in FIG. The rooting status of No. 14 was poor. There was evidence of mycelial implantation and mycorrhiza formation temporarily, but no white tufted hypha mass was visible.

Regarding the method of drilling a notch in the root of the host tree, a large-diameter automatic or manual drill is used instead of a saw or a sword, and the root diameter at the deepest portion is about 1 /
A method of providing a perforation hole up to the portion 3 may be used. In this way, the working efficiency is further increased because the saw or the blade of the sword is not damaged, and it is not necessary to dig a large amount of soil around the host tree vegetation.

(Second Example) In this example, the cultivated species of mycorrhizal mushrooms, Lyophyta (Lyophyta), was grown on the oak, which is a host tree of the family Fagaceae, by the method of inoculating the mycorrhizal fungi of the present invention.
llum shimeji), Shaka shimeji (Lyophyllum fumosu)
m) This is an example in which a fungus, Tricholoma bakamatsutake, was inoculated.

The oak, which is the host tree of the group in which the present example was performed (hereinafter referred to as the "exemplary group"),
Of those vegetated on similar terrain in terms of sunshine, drainage, slope, etc., 30 to 35 years old, 50 on the ground
Select one with a stem diameter of 30 cm inside or outside, and 1
At a distance of m, a root corresponding to the main root having a root diameter of about 6 cm at this point is selected, and 10 roots of such a root are selected.
Inoculum was inoculated in several places. Note that the Quercus serrata used as the host tree of the present example is a tree that has the property that roots penetrate deep into the ground, and is therefore less likely to fall.

In the inoculum inoculation method of this embodiment, as shown in FIGS. 1, 2 and 3, first, the upper surface of the root 1 to be inoculated and the three sides on both sides are sawed or Using a sword or the like, provide wedge-shaped notches 1a to 1c that match the size of the inoculum, and this wedge-shaped notch is the deepest part up to about 1/3 of the root diameter. The inoculated site is inoculated with a seed fungus mass 2 of 30 to 50 cm ³ , and the inoculated site is soil with low mycorrhizal fungi content, that is, mountain soil S containing no mulch layer.
Covered.

On the other hand, the control group of the present embodiment is similar to the first embodiment in that the technology according to the prior art Japanese Patent Application No. Hei 4-93743,
That is, a root cutting method is adopted, and a host tree Quercus serrata and its inoculated root part which are almost in the same vegetation state as that of the working group are selected, and ten such roots 10 per host tree are shown in FIG. And then inoculated with the seed fungus mass 12 having the same size of 30 to 50 cm ^{3 on} the cut surface 11 at the same time as the working group, and inoculated with the inoculated site in soil having a low mycorrhizal fungi content, that is, no mulch. It was covered with mountain soil S containing no layer.

According to the above method, the time from inoculation, that is, the selection of the root to be inoculated with the inoculum to the completion of the inoculation procedure was measured.
The average time required for inoculation work at each of the inoculation sites is 18 minutes per site for the control group, whereas it is 7 minutes for the execution group, which is much higher in the execution group than in the control group. It took less than half the time.

In general, the inoculation work has almost the same contents, and is often performed on a slope or a place away from a general road. Therefore, shortening the time required for the inoculation work directly means a reduction in labor. It is. Comparing the work content with the control group, the control group is cumbersome because a large amount of pebbled soil around the root must be removed because the saw is used before inoculation. At the same time, it takes a lot of hard work.

In the present example, the rooting status of the new root of the host tree Quercus serrata and the verification of mycorrhiza formation were performed one year after inoculation of the inoculum. As a result of the observation, it was observed that a large amount of new roots were found at the cutout perforated portion and the cut portion in both the execution group and the control group, and the new roots of 14 cm to 18 cm were elongated from these portions. . Regarding the mycorrhizalization of hon-shimeji mushrooms, white tufted hon-shimeji mushroom hyphae were found entangled in various places near the inoculum inoculation spot, but mycorrhization decreased as the tip of the new roots approached. , It was difficult to see.

In the present embodiment, the fungal species to be cultivated, Hon-shimeji mushroom and Shaka-shimeji mushroom, differ from mycorrhizal fungi of Matsutake mushrooms, and generally have less branching of new roots that are mycorrhiza. There is no black-brown discoloration, which is a finding that the stones have been implanted. However, for hon-shimeji mushrooms, if there is a stone that is exposed within 12 cm from the cutout drilling point and the root extension direction from the cutting point, the root is located beneath it. At the same time, the formation of white to gray-white mycelial soil masses, ie, whites, was visually recognized.
Within a range of 2 cm, the formation of white to off-white white was observed by integrating the soil, root and hypha.

In the third cultivation target fungus, Bakamatsutake, the root of the new root emerging from the cut-out portion and the cut portion, the branching of the new root was activated 1 to 2 cm behind the tip. And in this part, Tricholo
Black-brown discoloration, which is a finding indicating that the mycorrhiza of the genus ma was implanted, was confirmed. In addition, the clusters of tufted hyphae entangled with the root were remarkably visually recognized as being closer to the inoculation position.

(Third Example) In this example, Kochii, a beech host tree, was inoculated with the mycorrhizal fungi of the present invention by the inoculum method of the mycorrhizal fungi of the present invention.
holoma bakamatsutake). As a tree species, Kozii can find many very thick main roots near the ground, and it is relatively easy to search for inoculation. In the same manner as in the first and second embodiments, Koji, which is a host tree of the group in which the present embodiment is performed (hereinafter referred to as the "exemplary group"), is vegetated on a similar topography in terms of sunlight, drainage, inclination, and the like. Of those, 30-35 years old,
At the point 50 cm above the ground, three trunk diameters of 30 cm to 35 cm inside and outside are selected, and compared with the above two examples, at a point slightly farther from the tree body, that is, at a point 2 m away from the tree body, Root diameter is 5-10c among roots at the point
m roots were selected, and six inoculation sites were selected per host tree, and these were inoculated in April in spring.

As shown in FIGS. 1, 2 and 3, the inoculum inoculation method of this embodiment is similar to the first and second embodiments.
First, the upper surface of the root 1 to be inoculated and the three sides of both sides are provided with wedge-shaped notches 1a to 1c, which are adapted to the size of the inoculum using a saw or a sword. and up to about one-third portion of the root diameter deepest, 30 to 50 cm ³ in a wedge-shaped cutout portion or flaw 1a~c
Was inoculated, and the inoculated site was covered with soil having a low mycorrhizal fungi content, ie, mountain soil S containing no mulch layer at all.

On the other hand, the control group of this embodiment employs the technique according to Japanese Patent Application No. 4-93743, that is, the method of cutting the root, similar to the first and second embodiments. host trees cuspidata three, with the same vegetation state and select that inoculated roots, also 30 to 50 cm ³ the root 10 after cut as shown the six positions per host trees one in Figure 6, the cut surface 11 The inoculum 12 was placed and inoculated at the same time as the experimental group, and the inoculation site was covered with soil having a low mycorrhizal fungi content outside the cultivation target, that is, mountain soil S containing no mulch layer.

According to the above-mentioned method, when one year after the inoculation, the change in the vigor of the host tree was observed. As a result, two of the three trees of the control group showed a slope of the trunk. One-way roots were seen rising. In contrast, in the experimental group, there was no noticeable change in the vigor of all three host trees, and no visible abnormality was observed. At the same time and at the same time as the above-mentioned tree vigor observation, the rooting status of the new roots of each of the working group and the control group was investigated and the mycorrhization was verified. In the control group, where the stem is tilted and the roots are raised, the new roots that have emerged from the cut or inoculated sites are also
Many of these roots were cut, and no mycorrhizalization of the cultivated fungal species was observed in such new roots. In addition, among the roots in the opposite direction where the roots emerged, some of the new roots that should have rooted were cut off when the trunk was tilted and died.

Among the three control groups, one of which had no trunk inclination, and all the host trees of the experimental group,
A large amount of roots of new roots were observed at the notch drilling site, and the lengths of these new roots also showed considerable extension of 13 to 19 cm. In these new roots, branching of the new roots was actively observed 1-2 cm behind the tip, and a black-brown color indicating that the mycorrhiza of Tricholoma sp. Discoloration was confirmed.

[0060] Further, the closer to the inoculation position, the more noticeably the cluster of hyphal hypha entangled with the root. 6-1 from the inoculation site
At two points of 2 cm, it was confirmed that dura spores peculiar to Bakamatsu mushroom were blowing out so as to cover the surface of the exposed stone and the upper part of the soil. A lamellar metamorphosis was confirmed, which indicates the presence of a dural spore layer, the surface being white and the inside being light brown. When a part of the light brown dural spore layer was observed under a microscope, a finding very similar to the cultured dura spores of Bacillus matsutake was obtained.

(Fourth Embodiment) In this embodiment, the inoculation method of the mycorrhizal fungi of the present invention was used to inoculate Alaska, a host tree of the family Fagaceae, to the cultivated fungal species of mycorrhizal mushrooms (Ly).
ophyllum fumosum IFO 30662).

As in the case of the first to third embodiments, the arakashi, which is the host tree of the group in which the present embodiment was carried out (hereinafter referred to as the "working group"), was vegetated on a similar topography in terms of sunshine, drainage, inclination and the like. Of the 40-year-old trees, and select 5 host trees that have at least 4 roots at the point 1 m away from the tree body and have a root diameter of about 3-4 cm at this point. The seeds were inoculated to the roots of these four locations per host tree.

In the inoculum inoculation method of this embodiment, first, as shown in FIG. 5, which is a schematic diagram showing the shape of the inoculated portion at the time of inoculation of the inoculum, first, the portion corresponding to the skin layer and cambium of the root 1 is sawed and fleaed. Use and peel off. 30 to 5
A seed fungus mass 2 of 0 cm ³ was placed and inoculated, and this inoculated site was covered with soil having a low mycorrhizal fungi content, ie, mountain soil S containing no leaf layer at all.

The control group of this embodiment is the same as the control group of the first to third embodiments, and is similar to the conventional example of Japanese Patent Application No. 4-9374.
Taking the technique according to No. 3, that is, the method of cutting the root,
Arkashi host tree, which has almost the same vegetation status as the experimental group 5
Select four inoculated roots per book and one of them
6 was cut as shown in FIG. 6, and then a seed fungus mass 12 of 30 to 50 cm ³ was placed and inoculated on the cut surface 11 at the same time as the working group. The soil was covered with low soil, that is, mountain soil S containing no mulch layer.

According to the above method, the change in the vigor of the host tree was observed in March of the following year after the inoculation. As a result, three of the five Alaska trees of the control group showed a trunk inclination. One of them was tilted so that the branches and leaves came in contact with the ground, and the branch in one direction was completely raised. On the other hand, in the experimental group, all five host trees maintained a healthy vigor, and no visible abnormality was observed.

At the same time and at the same time as the above-mentioned tree vigor observation, the rooting status of the new roots of each of the working group and the control group was investigated and the mycorrhizalization was verified. In the host tree whose trunk is inclined in the control group, as shown in the control group in Example 3, the new root is cut in many cases, and such a new root contains bacteria of the cultivated species. No root formation was observed.

Of the five control groups, two of which did not show any inclination of the stem, and all the host trees of the working group,
A large amount of roots of new roots are observed at the notch drilling point, and the length of these new roots also shows a considerable extension of 14 to 17 cm, and the inoculation position, that is, the cutting point, 7 cm from the notch drilling point The soil, fine roots and mycelium are united in the range of, forming a cluster of hyphal hyphae, dense mycorrhizalization is visually recognized, and further, in the range of 12 cm from the inoculation position, branching of new roots is actively seen, and this A black-brown discoloration, which is a finding indicating that the mycorrhiza of Tricholoma spp. Was implanted, was confirmed in the portion, and the tip of the portion was yellowed and was found to be curled.

It is to be noted that, although iridescent trees such as soyogo have a slightly lower degree of mycelium bed formation than the beech family host trees,
Since the roots are wide and shallow, and the roots of fine roots have a good rooting characteristic, in the above-described two examples of the second and fourth examples, the cultivation target bacterial species is Honjimeji, In the case of Shaka shimeji, this property was used as an auxiliary means for forming a wide range of mycelial beds, and if it was arranged between host trees, the formation of dense mycelial beds or whites could be expanded in a wide range.

Among the mycorrhizal fungi, Steinpilz (Germany), c ■ pe (France) (European edible mushroom), Pfefferling (German apricot mushroom), C
hanterelle (French chanterelle), Funghi porcini
(From the Tuscany region of Italy) and other Japanese domestic botanicals such as boletus mushrooms, rushes, and broom mushrooms, as well as Tricholoma
The genus Nisematsutake and the like can also be the cultivation target bacterial species of the present invention.

[0057]

As described in detail above, according to the method for inoculating mycorrhizal fungi according to the present invention, a large amount of labor is not required when inoculating mycorrhizal fungi to be cultivated. The present invention provides a method for inoculating mycorrhizal mushrooms in which the growth of the mycelium is less likely to occur, mycelium implantation and mycorrhizal formation are ensured, and whites, that is, soil mycelium layers are formed smoothly.

In accordance with the method of inoculating the mycorrhizal fungi with the inoculum of the present invention, the characteristics of ilex trees such as Soyogo, which can be sub-primary host trees, are used as auxiliary means for forming a wide range of mycelial beds. The formation of a dense mycelial bed or white can be expanded in a wide range.

[Brief description of the drawings]

FIG. 1 is a schematic exploded end view of an inoculum inoculation root during an inoculation inoculation procedure according to the first to third embodiments of the present invention.

FIG. 2 is a schematic cross-sectional view of an inoculum root in the inoculum inoculation procedure according to the first to third embodiments of the present invention.

FIG. 3 is a schematic side perspective view of the root portion of the inoculum inoculation at the end of the inoculum inoculation procedure according to the first to third embodiments of the present invention.

FIG. 4 is a schematic view showing findings showing remarkable mycorrhization in the vicinity of the inoculum inoculation site at the root of the host tree of the first embodiment of the present invention.

FIG. 5 is a schematic view showing the shape of a site to be inoculated during a seed inoculation procedure according to a fourth embodiment of the present invention.

FIG. 6 is a schematic diagram showing a seed inoculation status of a conventional example.

FIG. 7 is a schematic view showing poor mycorrhiza formation near the inoculum inoculation site at the root of a host tree in a conventional example as a control group of the first example.

Claims (1)

(57) [Claims] 1. Excavating the ground on which a tree hosting mycorrhizal mushrooms is vegetated, exposing the roots of the host tree, inoculating the roots with mycorrhizal fungi to be cultivated, cultivation target excavation partially exposing the roots for seed inoculation
In the inoculum inoculation method of mycorrhizal fungi covered with soil having a low ectomycorrhizal fungi content, a notch of a predetermined width is formed around the root of the host tree, or a notch of an arbitrary shape is formed from the root surface. The part is a notch from the epidermis to the woody part, and it is preserved without giving a notch to a certain part of the woody part, and the notch or its vicinity is inoculated with mycorrhizal fungi inoculum. Inoculation method of mycorrhizal mushrooms.