JP2993253B2 - Method for producing animal fats and oils with low cholesterol content - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing animal fats and oils in which cholesterol is removed or reduced by removing cholesterol contained therein from animal fats and oils.

[0002]

2. Description of the Related Art In recent years, when a large amount of animal fat food such as meat and butter is consumed, cholesterol contained in animal fats and oils causes arteriosclerosis, myocardial infarction, stroke, etc. There is a demand for animal fats and oils foods in which the amount is reduced.

Conventionally, attempts to remove cholesterol contained in animal fats and oils have been made mainly in the food industry. For example, regarding the removal of cholesterol from milk fat, a supercritical gas extraction method (JP-A-62-13404)
2), a decomposition method using a microorganism or an enzyme (JP-A-57-86257) and the like have been proposed. However, these methods have complicated processing operations and require advanced technology and equipment, and have not been put into practical use in terms of cost.

[0004] A method of adsorbing and removing cholesterol using an adsorbent such as activated carbon has also been proposed (JP-A-61-136598), but it has not been put to practical use because it produces off-flavors and off-flavors.

[0005]

DISCLOSURE OF THE INVENTION The present invention provides a high-yield purified animal fat / oil having a reduced cholesterol content by industrially efficiently removing cholesterol from an animal fat / oil which could not be achieved by the conventional method. It is an object to provide a method for obtaining the information.

[0006]

Means for Solving the Problems The present invention has been made as a result of intensive studies to solve the above problems, and as a result, a simulated moving bed type chromatograph (hereinafter referred to as SMB) capable of continuously performing chromatographic separation. Using a mixed solvent system, especially a mixed solvent system consisting of hexane, ethanol, and water or a mixed solvent system consisting of hexane, acetone, ethanol, and water, and optimizing the operating conditions of SMB This is based on the finding that cholesterol can be removed from fats and oils.

In the conventional column chromatography, a mixed solvent system such as hexane and diethyl ether is used to fractionate cholesterol, and the mixing ratio is changed stepwise to convert triglyceride and cholesterol. It was sorted in stages. In addition, since this operation is performed batchwise, processing a large amount of samples in one operation requires a large-diameter column and a great deal of labor. However, by selecting an optimal solvent system using SMB, the cholesterol has been successfully removed from animal fats and oils continuously and efficiently.

[0008] The principle and apparatus of a simulated moving bed type chromatographic separation apparatus are known, and the operating conditions can be appropriately set according to any purity, processing amount, processing speed, concentration of the recovered liquid and the like. Generally, by increasing the ratio of the eluent to the raw material, the purity and the recovery rate of each are improved, but from the viewpoint of cost such as the amount of solvent used, the ratio is 4 or less, preferably 1-2. use.

The animal fats and oils used as raw materials in the present invention include lard, beef tallow, fish oil, milk fat and the like, as well as processed oils and fats such as butter, butter oil and hardened oil. These are dissolved in the above-mentioned organic solvent. The concentration is desirably about 10 to 70 W / V% from the viewpoint of processing efficiency. If this concentration is too high, separation will be poor,
If it is too low, the amount of the solvent used increases, which is not preferable. The mixing ratio of hexane, ethanol and water in the mixed solvent of the present invention is 100: 0 to 10: 0 to 0.1 and the like, and the mixing ratio of hexane, acetone, ethanol and water is 100: 0.
-20: 0-10: 0-0.1 is preferred.

[0010] The animal fats and oils with reduced cholesterol content obtained by the present invention can be obtained as foods and beverages with reduced cholesterol content by incorporating them into foods and beverages. In particular, butter or butter oil is used as an animal fat, and the cholesterol content in the oil is reduced.These dairy products are used as raw materials for the production of butter, margarine, reduced milk, cheese, ice cream, etc. When manufactured, dairy products with reduced cholesterol content can be obtained, making them useful as health foods.

Next, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.

[0012]

Example 1 500 g of butter oil was dissolved in 1000 ml of a hexane: ethanol: water mixed solution (100: 2: 0.02) to obtain an SMB treatment solution. Similarly, the eluent used was 4160 ml of a hexane: ethanol: water mixture (100: 2: 0.02). SMB is a 6-column type with a column diameter of 25 mm and a length of 460 mm.
120-S50 (YMC) was charged. The operating conditions were as follows: 1.91 ml / min of processing liquid supply, 7.93 ml / min of eluent supply, 2.72 ml / min of extract extraction, 7.11 ml / min of raffinate extraction, column temperature 40 ° C., step time 33.76 minutes. . As a result, 1430 ml of a cholesterol-containing fraction solution was obtained as an extract, and 3730 ml of a cholesterol-removed fraction solution was obtained as a raffinate. The raffinate is concentrated to dryness to reduce cholesterol-reducing fats and oils.
481 g was obtained.

The cholesterol-reducing fat and oil obtained in Example 1 and the raw material butter oil were dissolved in hexane, and the cholesterol content was measured by HPLC. The results are shown below.

[0014]

Example 2 200 g of lard was dissolved in 1000 ml of a mixture of hexane: ethanol: water (100: 2: 0.02) to obtain an SMB treatment solution. Similarly, 3650 ml of a mixture of hexane: ethanol: water (100: 2: 0.02) was used as the eluent. SMB is a 6-column type with a column diameter of 25 mm and a length of 460 mm.
0-S50 (YMC) was charged. The operating conditions were as follows: processing liquid supply amount 2.85 ml / min, eluent supply amount 10.37 ml / min, extract extraction amount 3.67 ml / min, raffinate extraction amount 9.56 ml / min, column temperature 40 ° C., step time 25.11 minutes. . As a result, 1290 ml of a cholesterol-containing fraction solution was obtained as an extract, and 3360 ml of a cholesterol-removed fraction solution was obtained as a raffinate. The raffinate is concentrated to dryness to reduce cholesterol-reducing fats and oils.
188 g were obtained.

[0015] The cholesterol-reducing oil and fat obtained in Example 2 and lard as a raw material were dissolved in hexane, and the cholesterol content was measured by HPLC. The results are shown below.

[0016]

Example 3 100 g of butter oil was dissolved in 1000 ml of a hexane: ethanol: water mixed solution (100: 2: 0.02) to obtain an SMB treatment liquid. Similarly, 3700 ml of a mixture of hexane: ethanol: water (100: 2: 0.02) was used as the eluent. SMB is a 6-column type with a column diameter of 25 mm and a length of 460 mm.
120-S50 (YMC) was charged. The operating conditions were as follows: processing solution supply rate 2.74 ml / min, eluent supply rate 10.14 ml / min, extract extraction rate 4.38 ml / min, raffinate extraction rate 8.50 ml / min, column temperature 40 ° C., step time 25.77.
Minutes. As a result, 1600 ml of a cholesterol-containing fraction solution was obtained as an extract and 3100 ml of a cholesterol-removed fraction solution was obtained as a raffinate. The raffinate was concentrated to dryness to obtain 481 g of a cholesterol-reducing oil and fat.

The cholesterol-reducing oil and butter oil obtained in Example 3 were dissolved in hexane, and the cholesterol content was measured by HPLC. The results are shown below.

[0018]

Reference Example 1 40% by weight of the cholesterol-reduced butter oil obtained in Example 1 and 60% by weight of skim milk were mixed and emulsified to prepare a reduced cream. Further, this reduced cream was cooled to 8 ° C. and aged for 8 hours, and then a cholesterol-reduced butter was produced using a conventional butter producing machine. The cholesterol-reducing butter produced here had a good flavor.

[0019]

Reference Example 2 81% by weight of the cholesterol-reduced butter oil obtained in Example 1, 17.6% by weight of skim milk and 1.4% by weight of salt were mixed, sterilized at 85 ° C, and further cooled to 60 ° C. This mixture was cooled with a closed-type surface scraping heat exchanger usually used for the production of margarine to obtain a cholesterol-reducing butter having a good taste.

[0020]

According to the method of the present invention, cholesterol in animal fats and oils can be removed in a short time by a simple operation, and animal fats and oils with reduced cholesterol content can be produced in high yield. Can be. Further, according to the method of the present invention, a large amount of animal fats and oils can be continuously treated, which is an industrially advantageous method.

Continuation of front page (72) Inventor Masanobu Jinbu 3-11-13 Tsurumai, Sakado-shi, Saitama (56) References JP-A-63-39991 (JP, A) JP-A-57-122099 (JP, A) Hei 3-100459 (JP, A) (58) Fields investigated (Int. Cl. ⁶ , DB name) C11B 3/10 A23D 9/007 C11B 3/00 G01N 30/44 G01N 30/46

Claims (2)

(57) [Claims] 1. An animal oil or fat comprising hexane, ethanol and
Mixed solvent of water or hexane, acetone, eta
Cholesterol-free animal oil / fat fraction obtained by dissolving in a mixed solvent consisting of phenol and water , supplying the mixture to a simulated moving bed type chromatographic separator, and separating it into a cholesterol-free animal oil / fat fraction and a cholesterol-containing fraction. A method for producing an animal fat or oil having a low cholesterol content, characterized by removing an organic solvent. 2. Animal fats and oils such as lard, beef tallow, fish oil and milk fat
The method according to claim 1, which is a processed oil or fat thereof .