JP2944662B1 - Rosacea treatment - Google Patents

Abstract

Abstract: [Object] To provide a remedy for rosacea which has no side effects and improves symptoms of rosacea. [Structure] A rosacea treatment agent with no side effects containing lactobacillus intracellular substance as an active ingredient, and improves rosacea-specific symptoms such as hot flush and hot sensation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a remedy for rosacea using the cell components of lactic acid bacteria as a raw material.

[0002]

BACKGROUND OF THE INVENTION Rosacea is a common disease in women, especially in middle-aged and older people. This disease can be a primary disease depending on the degree of clinical symptoms.
Degree (erythematous rosacea, forehead, telangiectatic erythema on cheek and dorsum of nose), second degree (mixed with acne rosacea, pore-matched papules and pustules) and third degree (nose nose, apex) Dark red mass and enlarged pores). Erythema on the face (red face)
Rosacea suffers from physical and mental distress, with significant external disturbances such as papules, pustules or rhinophyma, and apical masses, and increased warmth on the face due to changes in emotions and environmental temperature. Has brought.

[0003] The cause of rosacea is currently unknown. Allergies, digestive disorders, hair follicles, stress,
It has been suggested that the cause may be due to genetics, but no clear results have been obtained, and no fundamental treatment method for rosacea has been established to date. Therefore, at present, tetracycline antibiotics such as minocycline are used as symptomatic treatments, face washing is performed to maintain cleanliness, stress is eliminated, and vitamin B group supplementary administration is performed only. . However, minocycline cannot be used in many cases due to side effects such as vomiting, dermatitis, pigmentation, etc., and it is difficult for these treatments to improve the appearance disorder.

[0004] It is known that rosacea tends to worsen and become chronic without stopping the progress of its symptoms, because there is no fundamental treatment.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a drug which improves the appearance disorder of rosacea and has no side effects.

[0006]

It is known that lactic acid bacteria have various actions such as an immunostimulating action and a blood pressure lowering action. It is known that cells belonging to the genus Enterococcus, which is a kind of lactic acid bacteria, have an antiallergic effect (Japanese Patent Application No. H9-366257). Dermatitis (urticaria, erythema, etc.) caused by Candida bacteria that inhabit the intestinal tract
Is also known to have an improving effect (Japanese Unexamined Patent Publication No. H9-263539).

[0007] Further, with respect to the antiallergic action, it has been proved that the intracellular substance obtained by extracting the cells from the lactic acid bacteria cells themselves is more effective (Japanese Patent Application No. H9-36).
7257).

The present inventors have conducted a drinking test on rosacea patients who have become chronic and difficult to treat the cell components extracted from lactic acid bacteria. As a result, rosacea erythema, red face, papules, The pustules improved in a short time and revealed no side effects.

The lactic acid bacterium used in the present invention may be any lactic acid bacterium such as Lactobacillus, Bifidobacterium, Enterococcus or the like, and any lactic acid bacterium such as ATCC, IFO, JCM, etc. It can be obtained from domestic and international sales agencies. There is no risk of side effects because these cells are cells that are generally present in food, or cells that are used in food production, or cells that are isolated from the stool of healthy subjects. .

The lytic enzyme used in the present invention may be of any type, provided that it is commonly used to lyse bacteria such as actinase, zymolyase, chitalase, lysozyme, mutanolysin, and achromopeptidase. It is also possible to use a mixture of two or more enzymes. After the enzyme treatment, heat treatment is performed to completely extract the cell contents. The heat treatment may be performed at a temperature of 100 ° C. or higher. However, considering the extraction efficiency, a temperature (110 ° C. to 125 ° C.) at which the autoclave treatment can be performed is desirable. Further, the cell wall can be broken by using a physical method such as ultrasonic treatment or French press.

In order to prepare a lactic acid bacterium cell having its cell wall disrupted, a known carrier such as a carrier such as starch, lactose, and soybean protein, an excipient, a disintegrant, a lubricant, a stabilizer and a flavor corrector is used. It is formulated into tablets or granules by a well-known method using additives.

[0012] The amount used depends on symptoms, age, etc.
As an active ingredient, 0.002 to 0.1 g / kg body weight per day can be administered to an adult once or several times a day.

[0013]

[Embodiment 1] Method for preparing a bacteriolysis- and heat-treated cell sample from Enterococcus faecali
s) Inoculation of NF-1011 (Microtechnical Laboratories No. 12564) into Rogosa liquid medium having the following composition,
The cells were cultured for 16 hours. The obtained culture is centrifuged (12,
(000 × g, 20 minutes), and the cells were collected and washed twice with distilled water to obtain cells. The cells were suspended in distilled water, actinase was added at a final concentration of 0.1 mg / ml, treated at 37 ° C for 4 hours, and then heat-treated at 110 ° C for 10 minutes. The suspension was dried to obtain a dried cell sample (hereinafter referred to as a cell sample).

The composition of Rogosa liquid medium is shown. Trypticase 10 g Yeast extract 5 g Tryptose 5 g Monopotassium phosphate 3 g Dipotassium phosphate 3 g Triammonium citrate 2 g Tween 80 1 g Glucose 20 g Cysteine hydrochloride 0.2 g Salt solution (as per 1) 5 ml Distilled water 1000 ml (pH 7) After adjusting to 2.0, high-pressure steam sterilization at 121 ° C. for 15 minutes) (1) Salt solution: MgSO ₄ .7H ₂ O 11.5 g FeSO ₄ .7H ₂ O 0.7 g MnSO ₄ .2H ₂ O 2.4 g distilled water 100ml

Embodiment 2 FIG. Preparation of Ultrasonic Treated Cell Sample Enterococcus faecali
s) ATCC 19433T, Enterococcus faecium (Enteroc
occus faecium) ATCC19434T, Lactobacillus casei (Lacto
bacillus casei) ATCC393, Lactobacillus brevis (Lactobac
illus brevis) JCM 1059T, Bifidobacterium longum (Bifidob)
acterium longum) Each cell of JCM1217T was cultured in MRS medium (Difco) for 24 hours.
Cultured for hours. After completion of the culture, centrifugation (12,000 ×
g, 20 minutes), and the cells were collected and washed twice with distilled water to obtain the respective cells. These cells were placed in physiological saline (0.85
% Saline). This bacterial solution is treated with an ultrasonic crusher (Kubota 201M type) at a frequency of 9 KHz and a low temperature (5 to 7 ° C.) for 50 minutes to crush the cell wall, and then dried to obtain a dried bacterial cell sample. Was.

Embodiment 3 FIG. Clinical example 1 33-71 who visited a dermatological hospital, was diagnosed with rosacea, and had no effect even when treated with minocycline
2 to 4 g of a preparation containing the cell preparation prepared by the method of Example 1 in 14 year-old patients (cell preparation; 0.5 to 1.0 g)
Was consumed every day. 28 days later, the attending physician was effective,
The effect was determined in five stages: effective, somewhat effective, unchanged and worse.

Among the 14 patients, 4 (29% of the total) were judged to be highly effective, 2 (14% of the total) were judged to be effective, and 4 were judged to be slightly effective (29% of the total), the improvement rate was 72%. On the other hand, no patients were judged to have worsened their symptoms.

Embodiment 4 FIG. Clinical Example 2 25 volunteers who had been diagnosed as rosacea in the dermatology and had hot flushes were divided into 5 groups, and a capsule containing 0.5 g of each of the bacterial cells prepared by the method of Example 2 was prepared. 2
The tablets were taken daily. Twenty-eight days later, a questionnaire was made as to whether the flushing of the forehead, cheeks, and nasal muscles had improved compared to that before drinking as subjective symptoms.

The results of the questionnaire are shown below. Although there is a difference depending on the type of lactic acid bacteria, any of the lactic acid bacteria improved the hot flush, which is a symptom of rosacea.

From the above, the rosacea treatment agent of the present invention effectively works on rosacea patients who have not been completely cured even after treatment with minocycline, and reduces the rosacea symptoms such as hot flush and hot sensation on the face. It has been shown to improve or to improve the subjective symptoms of hot flush.

[0021]

EFFECTS OF THE INVENTION The cells of the lactic acid bacterium used in the agent of the present invention, in which the cell wall has been destroyed, have a strong effect of improving appearance symptoms and hot sensation against rosacea which is currently poorly treated. It can be used as a therapeutic agent. In addition, since there are no side effects, it can be taken with confidence and can be used in combination with the current treatment.

Continuation of the front page (58) Field surveyed (Int.Cl. ⁶ , DB name) A61K 35/74 CA (STN) JICST file (JOIS)

Claims (3)

(57) [Claims] 1. A remedy for rosacea comprising a cell component of a lactic acid bacterium as a main component. 2. The rosacea treatment agent according to claim 1, wherein the method of extracting cell components comprises subjecting the cells to a lysis treatment with one or more lytic enzymes, followed by heat treatment. 3. The agent for treating rosacea according to claim 1, wherein the method for extracting the cell components is a physical destruction method such as ultrasonic treatment.