JP2901057B2 - Non-insulin dependent diabetes animal feed - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a feed for an animal model of non-insulin-dependent diabetes mellitus.

[0002]

2. Description of the Related Art In recent years, experimental animals have been effectively utilized for elucidating various diseases. For example, mice, rats, guinea pigs, hamsters and the like are used for hypertension, immunodeficiency, muscular dystrophy, epilepsy and the like. Among them, diabetes is regarded as a very common disease in Japan,
Various studies are being conducted using experimental animals. Diabetes is a disease in which blood sugar is chronically increased due to metabolic abnormalities caused by insufficient action of insulin, and it is clear that genetic factors and physiological environment are involved in the onset, and analysis of genetic factors and effects of physiological environment is Many diabetes model animals are used. Diabetes is roughly classified into insulin-dependent diabetes as type I diabetes and non-insulin-dependent diabetes as type II diabetes, and can be divided into these two types in disease model animals.

[0003] In diabetes research, animals that develop the symptoms early are needed. Insulin-dependent diabetes model animals are affected by genetic factors.
Symptoms develop relatively quickly, but non-insulin-dependent diabetes model animals have genetic, immune, hormonal, etc.
In general, the onset of symptoms is slow because it is significantly different from insulin-dependent diabetes model animals. One of the causes is that the feed fed to the diabetic model animal is the same as that of a normal experimental animal. Therefore, development of a feed that hasten the onset of an insulin-independent diabetes model animal is awaited.

[0004]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a feed for an insulin-independent diabetes model animal, which promotes the early onset of diabetes.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above object, and found that the ratio of crude protein (CP) to digestible energy (DE) in feed was below a certain value. Or furthermore, it can be solved by adding a certain amount of saccharides.

That is, the present invention relates to (1) Crude protein in feed (C) against digestible energy (DE) in feed mainly composed of conventional raw materials such as cereals, rice bran, vegetable oil cakes and fats and oils.
P) ratio CP (%) / DE (kcal / g) of not more than 5.0, feed for animal model of insulin-independent diabetes, (2) cereals, rice bran, vegetable oil cake,
Ratio of crude protein (CP) in feed to digestible energy (DE) in feed mainly composed of conventional raw materials such as oils and fats CP
(%) / DE (kcal / g) is not more than 5.0 and saccharides are blended in not less than 10%, animal feed for an insulin-independent diabetes model animal, (3) sucrose and glucose are saccharides , Fructose, lactose, trihalose, galactose, and mannose. 2. The non-insulin-dependent diabetes animal feed according to (2).

Here, digestible energy (DE)
Is the crude energy of the feed minus the energy in the feces and indicates how much of the energy of the feed is used by the animal. The present inventors have studied cereals, rice bran,
Crude protein in feed (C) against digestible energy (DE) in feed mainly composed of conventional raw materials such as vegetable oil cakes and fats and oils
It has been found that early onset of diabetes can be promoted by setting the ratio CP (%) / DE (kcal / g) of P) to 5.0 or less, or by feeding a feed containing 10% or more of saccharides. Was.

Incidentally, CP (%) / DE (kcal / g)
Is preferably greater than 2.0. It is known that non-insulin-dependent diabetes develops due to changes in the physiological environment. Among physiological environments, the effect of the nutritional environment is significant, and the effect of feed is extremely large in experimental animals. When the feed of the present invention was fed to a non-insulin-dependent diabetic model animal, urine sugar was detected at an early age compared to a normal feed, and the blood sugar level also increased. Therefore, the feed of the present invention
It raises blood sugar and accelerates the appearance of urine sugar, and has a function to promote the onset of diabetes.

[0009] Next, since blood sugar is increased by ingestion of saccharides, the onset of diabetes can be promoted. Addition of at least 10% of saccharides such as sucrose, glucose, fructose, lactose, trihalose, galactose, mannose, etc. to the feed can increase the blood sugar level of the ingested animal and promote the onset of diabetes. It is possible. When the saccharide content is less than 10%, the effect is not apparent. There is a lot of research on diabetes, and there is a great demand for animals that have diabetes,
Since the onset of the disease takes a considerable amount of time, feeding the feed according to the present invention to accelerate the onset will play a part in furthering the research and development of diabetes.

[0010]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail with reference to Examples, but it should not be construed that the invention is limited thereto.

[0011]

EXAMPLES Feeds were manufactured mainly from conventional raw materials such as cereals, rice bran, vegetable oil cakes, oils and fats, etc. so as to have the components shown in Table 1, and tests were conducted. Nine male WBN / kob rats as non-insulin-dependent diabetes mellitus model animals were used in each group, and the feeds of Table 1 were fed from the age of 7 weeks to the age of 52 weeks. 13 weeks old, 26 weeks old, 39 weeks old, 52
At the age of three weeks, three animals in each group were tested for glucose tolerance test (GTT) and blood collection, and blood sugar level and blood biochemical properties were measured. Also, urine sugar of all animals was checked weekly from the start of the test,
The onset of diabetes was examined.

[0012]

[Table 1] Test feed ingredients

In the test plot, the ratio of the crude protein (CP) in the feed to the digestible energy (DE) in the feed according to the present invention is set to be not more than 5.0, ie, CP (%) / DE (kcal / g),
Sugar is added at 10%. Comparison areas 1 and 2
10% of sugar was not added. Further, the comparison groups 3, 4, and 5 have a CP (%) / DE (kcal / g) of 5.0 or more. The results are shown in Table 2 and Table 3.
It was shown to. As can be seen from [Table 2], in the test plot, the blood glucose level was higher than in the control plots 3.4.5 (excluding the age of 13 weeks). In [Table 3], the test plot was higher than the control plot. Urine sugar was detected from a low age, indicating that the onset of diabetes was promoted. In the test plot containing 10% sugar, the ratio of crude protein (CP)
(%) / DE (kcal / g) is 5.0 or less,
The onset was further promoted as compared with Comparative Groups 1 and 2 to which no sugar was added.

[0014]

[Table 2] Blood glucose measurement results (mg / 100ml)

[0015]

[Table 3] Diabetes incidence from urinary glucose measurement results

[0016]

The feed for experimental animals according to the present invention can be applied to non-insulin-dependent diabetic model animals to promote the onset of diabetes and contribute to the research and development of diabetes. It is effective.

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Claims (3)

(57) [Claims] 1. Digestible energy (DE) in feed mainly composed of conventional raw materials such as cereals, rice bran, vegetable oil cakes, oils and fats, etc.
Of crude protein (CP) in feed to the ratio CP (%) / DE
(Kcal / g) is 5.0 or less, a feed for an insulin-independent diabetes model animal. 2. Digestible energy in feed (DE) mainly composed of conventional raw materials such as cereals, rice bran, vegetable oil cakes and fats and oils.
Of crude protein (CP) in feed to the ratio CP (%) / DE
(Kcal / g) is 5.0 or less, and a saccharide is blended at 10% or more. 3. The feed for an insulin-independent diabetes model animal according to claim 2, wherein the saccharide is any one or more of sucrose, glucose, fructose, lactose, trihalose, galactose, and mannose.